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Nádia A Campos, Rony Swennen, Sebastien C Carpentier
Proteomics has been applied with great potential to elucidate molecular mechanisms in plants. This is especially valid in the case of non-model crops of which their genome has not been sequenced yet, or is not well annotated. Plantains are a kind of cooking bananas that are economically very important in Africa, India and Latin America. The aim of this work was to characterize the fruit proteome of common dessert bananas and plantains and to identify proteins that are only encoded by the plantain genome. We present the first plantain fruit proteome...
January 15, 2018: Proteomics
Ramith Ramu, Prithvi S Shirahatti, K R Anilakumar, Shivasharanappa Nayakavadi, Farhan Zameer, B L Dhananjaya, M N Nagendra Prasad
Background: The assessment of the nutritional composition and phytochemical screening of banana pseudostem (PB) and flower (FB) advocate this nonconventional food source for routine consumption, considering its various health benefits. Objectives: The aim is to assess the proximate nutrient composition, fatty acids, minerals, amino acid profile, and global antioxidant response (GAR) of PB and FB. Methods: Standard analytical procedures were used to determine the nutritional quality and GAR of PB and FB...
December 2017: Pharmacognosy Research
Jasna Nikolić, Andrijana Nešić, Skadi Kull, Frauke Schocker, Uta Jappe, Marija Gavrović-Jankulović
Diagnostic reagents based on food allergen extracts often lack sufficient sensitivity. The introduction of well characterized food allergens in molecular allergy diagnosis has been recognized as valid approach to circumvent unstandardized allergen extracts. Banana fruit (Musa acuminata) is a well-established allergen source which besides six characterized allergens, contains unidentified IgE reactive proteins whose clinical relevance remains undefined. By employment of a combinatorial peptide ligand library (CPLL) methodology with 2-D PAGE, mass spectrometric and 2-D immunoblot analysis, a novel allergen from banana fruit was detected in banana as catalase...
January 10, 2018: Journal of Proteomics
Yun Ge, Kang-Di Hu, Sha-Sha Wang, Lan-Ying Hu, Xiao-Yan Chen, Yan-Hong Li, Ying Yang, Feng Yang, Hua Zhang
[This corrects the article DOI: 10.1371/journal.pone.0180113.].
2018: PloS One
Michael D Kendig, Robert A Boakes, Laura H Corbit
Cue-potentiated feeding (CPF) describes the stimulation of food consumption by cues that have become associated with food. Determining under what Conditions CPF occurs is important to better understand how exposure to food cues contributes to overeating. CPF is typically found to be specific: cues enhance consumption only of the food they have signaled. Further, previous research has focused largely on discrete cues rather than multimodal cues such as a feeding environment. The present experiments paired a "Plus" context with highly palatable food and a "Minus" context with no food or chow in adult female rats...
January 2018: Journal of Experimental Psychology. Animal Learning and Cognition
Kwaku Aduse-Poku, Freerk Molleman, William Oduro, Samuel K Oppong, David J Lohman, Rampal S Etienne
The unified neutral theory of biodiversity and biogeography has gained the status of a quantitative null model for explaining patterns in ecological (meta)communities. The theory assumes that individuals of trophically similar species are functionally equivalent. We empirically evaluate the relative contribution of neutral and deterministic processes in shaping fruit-feeding butterfly assemblages in three tropical forests in Africa, using both direct (confronting the neutral model with species abundance data) and indirect approaches (testing the predictions of neutral theory using data other than species abundance distributions)...
January 2018: Ecology and Evolution
Ayşe Sağmak Tartar, Ayhan Akbulut, Ömür Gökmen Sevindik, Hatice Handan Akbulut, Kutbeddin Demirdağ
In our country, within years, despite a dramatic drop in the number of patients with malaria, a dramatic increase in the number of patients with import malaria is noteworthy. A 32-year-old male patient presented with fever, shivering, malaise, and loss of appetite. He had travelled to West Africa. Laboratory findings were as follows: hemoglobin: 8.8 g/dL and anti-HIV: positive. Microscopic examinations of thin blood smears and thick blood preparations revealed widespread trophozoites. The presence of double-dotted ring forms and banana-shaped gametocytes resulted in Plasmodium falciparum malaria being diagnosed...
December 2017: Türkiye Parazitolojii Dergisi
Aditya Jielella Reddy, Ashok Kumar Dubey, Shailendra Handu, Manocha Sachin, Pramod Kumari Mediratta, Qazi Ahmed Mushtaq
Objective: The Musa sapientum (banana) plant extract has shown antioxidant activity in previous studies. Oxidative stress is one of the important factors implicated in the pathogenesis of anxiety disorders. The present study aimed to evaluate the anxiolytic activity of aqueous extract of M. sapientum stem (MSSE) in experimental models in mice. Material and Methods: Elevated Plus Maze method and locomotor monitoring by photoactometer were used. Animals were divided into five different groups (n=6/group)...
November 2017: Avicenna Journal of Phytomedicine
A Bernal, O Simón, T Williams, D Muñoz, P Caballero
BACKGROUND: A Chrysodeixis chalcites nucleopolyhedrovirus from the Canary Islands (ChchNPV-TF1) has proved to be effective for control of this pest on banana crops. Commercialization of this virus as a bioinsecticide requires an efficient production system. RESULTS: The most suitable larval instar for virus production was the sixth instar (L6 ), which produced 1.80x1011 occlusion bodies (OBs)/larva and showed a lower prevalence of cannibalism (5.4%) than fourth (L4 ) or fifth (L5 ) instars...
January 3, 2018: Pest Management Science
Fuhong Cai, Dan Wang, Min Zhu, Sailing He
Spectrally-resolved imaging techniques are becoming central to the investigation of bio-samples. In this paper, we demonstrate the use of a WIFI-camera as a detection module to assemble a pencil-like imaging spectrometer, which weighs only 140 g and has a size of 3.1 cm in diameter and 15.5 cm in length. The spectrometer is standalone, and works wirelessly. A smartphone or network computer can serve as the data receiver and processor. The wavelength resolution of the spectrometer is about 17 nm, providing repeatable measurements of spatial two-dimensional images at various wavelengths for various bio-samples, including bananas, meat, and human hands...
December 1, 2017: Biomedical Optics Express
Christopher A Bell, Howard J Atkinson, Alan C Andrade, Hoa X Nguyen, I Gede Swibawa, Catherine Lilley, James McCarthy, Peter Urwin
Coffee yields are adversely affected by plant-parasitic nematodes and the pathogens are largely under-reported because a simple and reliable identification method is not available. We describe a PCR-based approach to rapidly detect and quantify the major Pratylenchus and Meloidogyne nematode species that are capable of parasitising coffee. The procedure was applied to soil samples obtained from a number of coffee farms in Brazil, Vietnam and Indonesia to assess the prevalence of these species associated both with coffee (Coffea arabica and C...
January 1, 2018: Phytopathology
Pilar Escalante-Minakata, Vrani Ibarra-Junquera, José de Jesús Ornelas-Paz, Victoria García-Ibáñez, José J Virgen-Ortíz, Apolinar González-Potes, Jaime D Pérez-Martínez, Mario Orozco-Santos
This work presents a novel method to associate the polyphenol oxidase (PPO) and the peroxidase (POD) activities with the ripening-mediated color changes in banana peel and pulp by computational image analysis. The method was used to follow up the de-greening of peel and browning of homogenized pulp from 'Giant Dwarf' (GD: Musa AAA, subgroup Cavendish) and FHIA-23 (tetraploid hybrid, AAAA) banana cultivars. In both cultivars, the color changes of peel during the ripening process clearly showed four stages, which were used to group the fruit into ripening stages...
January 2018: 3 Biotech
Chuanqi Xie, Bingquan Chu, Yong He
This study investigated the feasibility of using hyperspectral imaging for determining banana color (L∗, a∗ and b∗) and firmness as well as classifying ripe and unripe samples. The hyperspectral images at wavelengths 380-1023nm were acquired. Partial least squares (PLS) models were built to predict color and firmness. Two-wavelength combination method λi-λjλi+λj,λi2-λj2λi2+λj2,λiλjandλi-λj was used to identify the effective wavelengths. Based on the selected wavelengths, PLS models obtained good results with the coefficient of determination in prediction (Rp2) of 0...
April 15, 2018: Food Chemistry
Jaindra Tripathi, Jonathan Matheka, Ibsa Merga, Endale Gebre, Leena Tripathi
Enset (Ensete ventricosum (Welw.) Cheesman) is an economically important staple food crop in Ethiopia, especially in the southern and southwestern regions. It is called "false banana" due to its resemblance to banana, but inability to produce any edible fruit. The crop is clonally propagated using field-grown suckers. This study reports the development of a robust regeneration technique to propagate large numbers of plantlets using corm discs containing intercalary meristematic tissues. Hundreds of shoot buds were induced from corm discs of enset cultivar 'Bedadeti' cultured on Murashige and Skoog (MS) medium supplemented with 1...
2017: In Vitro Cellular & Developmental Biology. Plant: Journal of the Tissue Culture Association
Yohan Liber, Clarisse Létondor, Sophie Pascal-Lorber, François Laurent
Because of its high persistence in soils, t1/2=30years, chlordecone (CLD) was classified as a persistent organic pollutant (POP) by the Stockholm Convention in 2009.The distribution of CLD over time has been heterogeneous, ranging from banana plantations to watersheds, and contaminating all environmental compartments. The aims of this study were to (i) evaluate the potential of Miscanthus species to extract chlordecone from contaminated soils, (ii) identify the growth parameters that influence the transfer of CLD from the soil to aboveground plant parts...
December 20, 2017: Science of the Total Environment
Nobutake Nakatani, Alexander Mosqueda, Joan Marc Cabot, Estrella Sanz Rodriguez, Kunio Yoshikawa, Brett Paull
A simple and rapid capillary electrophoresis method with capacitively coupled contactless conductivity detection (CE-C4 D) for the simultaneous determination of inorganic and organic anions in liquid product obtained from the hydrothermal treatment of biomass residues is presented. Under the optimal analytical conditions, limits of detection ranged from 1.8 to 9.4 μM for most target solutes and 53 μM for citrate. Relative standard deviations were below 0.5% for migration times and within 0.6-6.5% for peak areas for all solutes...
December 23, 2017: Electrophoresis
Idan Niv, Efi Efrati
Bent core (or banana shaped) liquid-crystal-forming-molecules locally favor an ordered state of zero splay and constant bend. Such a state, however, cannot be realized in the plane and the resulting liquid-crystalline phase is frustrated and must exhibit some compromise of these two mutually contradicting local intrinsic tendencies. This constitutes one of the most well-studied examples in which the intrinsic geometry of the constituents of a material gives rise to a geometrically frustrated assembly. Such geometric frustration is not only natural and ubiquitous but also leads to a striking variety of morphologies of ground states and exotic response properties...
December 20, 2017: Soft Matter
Taotao Li, Qixian Wu, Yong Wang, Afiya John, Hongxia Qu, Liang Gong, Xuewu Duan, Hong Zhu, Ze Yun, Yueming Jiang
Fusarium proliferatum is an important pathogen and causes a great economic loss to fruit industry. Environmental pH-value plays a regulatory role in fungi pathogenicity, however, the mechanism needs further exploration. In this study, F. proliferatum was cultured under two initial pH conditions of 5 and 10. No obvious difference was observed in the growth rate of F. proliferatum between two pH-values. F. proliferatum cultured under both pH conditions infected banana fruit successfully, and smaller lesion diameter was presented on banana fruit inoculated with pH 10-cultured fungi...
2017: Frontiers in Microbiology
Hayley Ash, Tessa E Smith, Simon Knight, Hannah M Buchanan-Smith
Cortisol levels are often used as a physiological measure of the stress response in captive primates, with non-invasive measures of this being an important step in welfare assessment. We report a method of collecting saliva samples voluntarily from unrestrained captive common marmosets (Callithrix jacchus), and validate an enzyme-linked immunosorbent assay (ELISA) technique previously unused in this species. Saliva samples were collected from marmosets housed in pairs in a UK laboratory. The assay showed parallelism, precision, accuracy and sensitivity, meeting the criteria typically used to investigate the effectiveness of new analytical techniques...
December 14, 2017: Physiology & Behavior
Huafang Liang, Yin Lau, Ting Ting Zhou, Xiaoyuan Li, Bin Li, Siuming F Chan
The Molt Inhibiting Hormone gene and cDNA of the banana shrimp Fenneropenaeus merguiensis (FmMIH1) has been cloned and characterized. FmMIH1 possesses most of the characteristics of the eyestalk CHH/MIH/GIH family subtype-II neuropeptides. FmMIH1 open reading frame consists of 315 bp encoding for 105 amino acid residues. The mature peptide of FmMIH1 consists of 76 amino acid residues, a glycine residue at position 11 of the mature peptide and 6 cysteine residues located in the conserved position. In addition to eyestalk, high levels of FmMIH1 transcript could also be detected in the intestine...
December 13, 2017: Peptides
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