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https://www.readbyqxmd.com/read/28804942/metagenomic-alkaline-protease-from-mangrove-sediment
#1
Tharcilla B A Pessoa, Rachel P Rezende, Eric de Lima Silva Marques, Carlos P Pirovani, Thalis F Dos Santos, Ana C Dos Santos Gonçalves, Carla C Romano, Natielle C Dotivo, Ana C O Freitas, Luiz C Salay, João C T Dias
Functional screening of metagenomic libraries is an important tool for the discovery of new molecules. The metabolic diversity of microorganisms enables survival in harsh environments and is related to the production of enzymes. In this study, we identified a protease-producing clone from a metagenomic library derived from mangrove sediment. The protease was purified by ammonium sulphate precipitation and gel filtration chromatography, with a yield of 77.27% and a specific activity of 8.57 U μg(-1) . It had a molecular weight of approximately 70 kDa...
August 14, 2017: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/28804675/transition-in-dental-treatment-utilization-in-jammu-and-kashmir-india-a-10-year-retrospective-study
#2
Aasim Farooq Shah, Manu Batra, A Ishrat
BACKGROUND: Utilization of health services is the actual attendance by the members of the public at health care facilities to receive care. Utilization, serves as an important tool for oral health policy decision-making. The aim of the present study was to retrospectively gauge and assess the utilization pattern of the dental treatments which was given in hospital for last 10 years. MATERIALS AND METHODS: This retrospective infirmary based study was carried out at Sheri Kashmir Institute of Medical Sciences (SKIMS), Srinagar, Jammu and Kashmir, India...
December 2016: Nepal journal of epidemiology
https://www.readbyqxmd.com/read/28800577/case-report-use-of-tumor-and-germline-y-chromosomal-analysis-to-guide-surgical-management-in-a-46-xx-female-presenting-with-gonadoblastoma-with-dysgerminoma
#3
Jeanna M McCuaig, Abdul Noor, Barry Rosen, Robert F Casper, Frederic Mitri, Terence Colgan, Raymond H Kim
Gonadoblastomas are rare mixed gonadal tumors that are almost always found in individuals with 46, XY karyotype or some other form of Y chromosome mosaicism. It is extremely rare to diagnose gonadoblastoma in phenotypically normal 46, XX females. Herein, we present a 20-year-old 46, XX female diagnosed with gonadoblastoma and dysgerminoma. Use of cytogenetic and molecular analyses to identify the presence of Y chromosome material in peripheral blood, gonadal, and tumor tissue can exclude mosaicism to provide reassurance to undertake conservative surgical management and preserve fertility...
September 2017: International Journal of Gynecological Pathology
https://www.readbyqxmd.com/read/28798089/effect-of-continuous-positive-airway-pressure-on-blood-pressure-and-metabolic-profile-in-women-with-sleep-apnoea
#4
Francisco Campos-Rodriguez, Monica Gonzalez-Martinez, Angeles Sanchez-Armengol, Bernabe Jurado-Gamez, Jose Cordero-Guevara, Nuria Reyes-Nuñez, Maria F Troncoso, Araceli Abad-Fernandez, Joaquin Teran-Santos, Julian Caballero-Rodriguez, Mercedes Martin-Romero, Ana Encabo-Motiño, Lirios Sacristan-Bou, Javier Navarro-Esteva, Maria Somoza-Gonzalez, Juan F Masa, Maria A Sanchez-Quiroga, Beatriz Jara-Chinarro, Belen Orosa-Bertol, Miguel A Martinez-Garcia
Continuous positive airway pressure (CPAP) reduces blood pressure levels in hypertensive patients with obstructive sleep apnoea (OSA). However, the role of CPAP in blood pressure and the metabolic profile in women has not yet been assessed. In this study we investigated the effect of CPAP on blood pressure levels and the glucose and lipid profile in women with moderate-to-severe OSA.A multicentre, open-label, randomised controlled trial was conducted in 307 women diagnosed with moderate-to-severe OSA (apnoea-hypopnoea index ≥15 events·h(-1)) in 19 Spanish Sleep Units...
August 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28796443/relative-cerebral-blood-volume-is-a-potential-biomarker-in-late-delayed-radiation-induced-brain-injury
#5
Ying Xie, Haiwei Huang, Junjie Guo, Dongxiao Zhou
PURPOSE: To assess whether relative cerebral blood volume (rCBV) can provide information to reliably evaluate the stages of late delayed radiation-induced brain injury. MATERIALS AND METHODS: Forty patients diagnosed with late delayed radiation-induced brain injury were enrolled. The patients were examined using a 1.5T magnetic resonance imaging (MRI) system equipped with an 8-channel head coil. An echo planar imaging (EPI) sequence was used in perfusion-weighted imaging (PWI)...
August 10, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28792915/fast-rbc-loading-by-fluorescent-antibodies-and-nuclei-staining-dye-and-their-potential-bioanalytical-applications
#6
Mohamed K Al-Essa, Susanne Melzer, Attila Tarnok, Kamal A Hadidi, Mohammed El-Khateeb
This study was designed to load different antibodies (Abs) and a fluorescent dye onto the red blood cell (RBC) surface. We have used fluorescein isothiocyanate (FITC)-conjugate anti-human Ab, CD22-PE (B-cell marker-phycoerythrin Ab), and 4',6-diamidino-2-phenylindole (DAPI) for insertion over the RBC surface. In a first step, conjugation experiments were performed: in dimethyl sulfoxide (DMSO), RBCs were conserved and modified by succinic anhydride to create an additional -COOH group, and then activated with 3-(3-dimethylaminopropyl)carbodiimide-N-hydroxysuccinimide (EDC-NHS) in 2-(N-morpholino) ethanesulfonic acid hydrate buffer for insertion of labeled Abs or DAPI...
August 9, 2017: Zeitschrift Für Naturforschung. C, A Journal of Biosciences
https://www.readbyqxmd.com/read/28792100/understanding-sources-of-methylmercury-to-songbirds-with-stable-mercury-isotopes-challenges-and-future-directions
#7
Martin Tsz-Ki Tsui, Evan M Adams, Allyson K Jackson, David C Evers, Joel D Blum, Steven J Balogh
Mercury (Hg) stable isotope analysis is an emerging technique that has contributed to a better understanding of many aspects of the biogeochemical cycling of Hg in the environment. However, no study has yet evaluated its usefulness in elucidating the sources of methylmercury (MeHg) to songbird species, a common organism for biomonitoring of Hg in forested ecosystems. In this pilot study, we examined stable mercury isotope ratios in blood of four species of songbirds and the invertebrates they are likely foraging upon in multiple habitats in a small watershed of mixed forest and wetlands in Acadia National Park in Maine, U...
August 9, 2017: Environmental Toxicology and Chemistry
https://www.readbyqxmd.com/read/28790006/the-relationship-between-the-direction-of-putaminal-intracerebral-hemorrhage-and-anterior-cerebral-artery-predominance
#8
Jae-Ho Lee, Chul-Hoon Chang, Young-Jin Jung, Jong-Hoon Kim
INTRODUCTION: It is expected that anatomical variations in the circle of Willis have a direct effect on blood flow in the internal carotid artery. Rupture of the lenticulostriate artery of the middle cerebral artery 1st segment (M1) is the most common cause of putaminal intracerebral hemorrhage(ICH), and we hypothesized that this could be related to the anatomical variations of the circle of Willis and the predominance of the anterior cerebral artery 1st segment (A1). METHODS: We retrospectively reviewed the records of 544 patients who were treated for spontaneous ICH between 2013 and 2016 at Yeungnam University Hospital...
August 5, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28782747/laparoscopic-surgery-for-solitary-insulinoma-in-the-absence-of-ious
#9
Abhay Narendra Dalvi, Mahadeo Namdeo Garale, Yogesh Prabhakar Takalkar, Sameer Ashok Rege, Pinky Manoharlal Thapar, Lila Anurag, Nalini Samir Shah
BACKGROUND: Insulinomas are the most common pancreatic neuroendocrine neoplasms. In spite of adequate pre-operative localisation, conventional surgical methods rely on intraoperative palpation. Intraoperative ultrasonography (IOUS) is said to aid in accurate localisation, decreases morbidity. Laparoscopic removal of pancreatic endocrine neoplasms is beneficial due to magnification and minimal invasion; however, in the absence of IOUS, error of judgement may lead to conversion to open surgery, thereby relying on 'palpation method' to localise the tumour...
August 1, 2017: Journal of Minimal Access Surgery
https://www.readbyqxmd.com/read/28782399/use-of-bakri-balloon-tamponade-bbt-for-conservative-management-of-postpartum-haemorrhage-a-tertiary-referral-centre-case-series
#10
Manisha Mathur, Qiu Ju Ng, Shephali Tagore
The aim of this study was to investigate the efficacy of the Bakri balloon in the management of PPH. This was a retrospective review of 49 patients, who had Bakri balloon inserted for PPH in KK Hospital between April 2013 and December 2015. The main outcome measure was achievement of haemostasis by Bakri balloon tamponade (BBT). Our success rate was 81.6%. Out of the nine failures (18.0%), five (55.6%) had subtotal hysterectomies and four (44.4%) had total hysterectomies. The causes of PPH in these nine women were unsuspected or foci of placenta accreta (55...
August 6, 2017: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/28777390/thrombosis-in-left-ventricular-assistance-device-with-centrifugal-technology-is-early-thrombolysis-a-better-solution
#11
Paolo Centofanti, Andrea Baronetto, Matteo Attisani, Davide Ricci, Erika Simonato, Michele W La Torre, Massimo Boffini, Mauro Rinaldi
BACKGROUND: Continuous flow left ventricular assistance devices (CF-LVADs) have revolutionized the treatment of advanced heart failure. Pump replacement for thrombosis is a high-risk procedure with a high perioperative mortality rate with possible recurrence. We aim to summarize our experience using a conservative approach with medical therapy. METHODS: We retrospectively reviewed records of patients who experienced pump thrombosis after LVAD implantation with HeartWare HVAD at our institution, from November 2010 to March 2016...
July 27, 2017: International Journal of Artificial Organs
https://www.readbyqxmd.com/read/28777043/diagnostic-potential-of-monoclonal-antibodies-developed-against-c-terminal-polypeptide-of-p-falciparum-histidine-rich-protein2-pfhrp2-in-malaria-infected-patients-from-india
#12
Reena Verma, Sara Chandy, N S Jayaprakash, Anand Manoharan, M A Vijayalakshmi, Krishnan Venkataraman
Malaria, caused by Plasmodium falciparum has become a major health burden in most tropical and developing countries. P. falciparum Histidine Rich Protein2 (PfHRP2), which exhibits polymorphism, is being widely used as a diagnostic marker. Recently, we reported the development of monoclonal antibodies against conserved C-terminal 105 amino acids of PfHRP2 for malaria diagnosis. Now, in this study, the diagnostic performance of two anti-C-terminal PfHRP2 mAbs (b10c1 and Aa3c10) were evaluated with 100 blood samples from clinically identified malaria patients from seven different geographical centers in India...
August 4, 2017: Pathogens and Global Health
https://www.readbyqxmd.com/read/28776215/-cavernomas-of-the-central-nervous-system-observational-study-of-111-patients
#13
C Mayer, U M Mauer, G Bluhm, R Mathieu, C Hackenbroch, S Mayer
PURPOSE: The prevalence of cavernomas (cavernous hemangioma) is 0.1-0.7%. Cavernomas are often found as an incidental finding within the framework of magnetic resonance imaging (MRI) examinations in mainly young and healthy patients. In the literature, the reported risk of hemorrhage varies greatly and is sometimes higher than that of aneurysms, which is surprising given that cavernomas are part of the low blood pressure system. After the diagnosis the medical practitioner and the patient have to decide on the further therapy, either surgical removal or the strategy of watchful waiting (conservative treatment)...
August 3, 2017: Der Nervenarzt
https://www.readbyqxmd.com/read/28774217/planned-cesarean-hysterectomy-versus-modified-form-of-segmental-resection-in-patients-with-placenta-percreta
#14
Cetin Kilicci, Enis Ozkaya, Ahmet Eser, Evrim Ergen Bostanci, Ilhan Sanverdi, Cigdem Abide Yayla, Elif Tozkir, Semra Kayatas Eser
OBJECTIVES: The aim of this study was to compare some clinical characteristics of two different management alternatives in pregnants with placental invasion anomalies. METHODS: We conducted a single-center retrospective study of all patients who delivered with invasive placentation between January 2016 and May 2017. We included only the patients with placental invasion anomaly and planned cesarean section. RESULTS: Fifty-one pregnants met the inclusion criteria...
August 3, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28771210/coral-derived-natural-marine-compound-gb9-impairs-vascular-development-in-zebrafish
#15
Yi-Chun Song, Bao-Jueng Wu, Chien-Chih Chiu, Chun-Lin Chen, Jun-Qing Zhou, Shuo-Rong Liang, Chang-Yih Duh, Ping-Jyun Sung, Zhi-Hong Wen, Chang-Yi Wu
Blood vessels in vertebrates are established and genetically controlled in an evolutionarily-conserved manner during embryogenesis. Disruption of vascular growth by chemical compounds or environmental hormones may cause developmental defects. This study analyzed the vascular impacts of marine compound GB9 in zebrafish. GB9 was isolated from the marine soft coral Capnella imbricata and had shown anti-neuroinflammatory and anti-nociceptive activities. However, the role of GB9 on vascular development has not been reported...
August 3, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28766818/the-malaria-parasite-plasmodium-relictum-in-the-endemic-avifauna-of-eastern-cuba
#16
Letícia Soares, Peter Marra, Lindsey Gray, Robert E Ricklefs
Island populations are vulnerable to introduced pathogens, as evidenced by extinction or population decline of several endemic Hawaiian birds caused by the malaria parasite, Plasmodium relictum (order Haemosporida). We analyzed blood samples from 363 birds caught near Guantánamo Bay, Cuba, for the presence of haemosporidian infections. We characterized parasite lineages by determining nucleotide variation of the parasite's mitochondrial cyt b gene. Fifty-nine individuals were infected, and we identified 7 lineages of haemosporidian parasites...
August 2, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/28766274/pathogen-or-contaminant-distinguishing-true-infection-from-synovial-fluid-culture-contamination-in-patients-with-suspected-septic-arthritis
#17
Mary Louise Fowler, Clara Zhu, Kevin Byrne, Sarah B Lieber, Andrew Moore, Robert H Shmerling, Ziv Paz
BACKGROUND: Isolation of bacteria from synovial fluid (SF) is the gold standard for diagnosis of septic arthritis (SA). Contamination results in misdiagnosis and mismanagement. This study identifies clinical characteristics, microbiology, and outcomes of patients with contaminated SF and compares them with patients with true SA. METHODS: We conducted a retrospective study including all patients aged 18 and older admitted to a single, tertiary-care hospital between 1998 and 2015 with suspected SA and positive SF cultures...
August 1, 2017: Infection
https://www.readbyqxmd.com/read/28765838/sex-specific-molecular-responses-of-quick-to-court-protein-in-indian-malarial-vector-anopheles-culicifacies-conflict-of-mating-versus-blood-feeding-behaviour
#18
Tanwee Das De, Punita Sharma, Charu Rawal, Seena Kumari, Sanjay Tavetiya, Jyoti Yadav, Yasha Hasija, Rajnikant Dixit
Understanding the molecular basis of mosquito behavioural complexity plays a central role in designing novel molecular tools to fight against their vector-borne diseases. Although the olfactory system plays an important role in guiding and managing many behavioural responses including feeding and mating, but the sex-specific regulation of olfactory responses remain poorly investigated. From our ongoing transcriptomic data annotation of olfactory tissue of blood fed adult female An. culicifacies mosquitoes; we have identified a 383 bp long unique transcript encoding a Drosophila homolog of the quick-to-court protein...
July 2017: Heliyon
https://www.readbyqxmd.com/read/28761594/case-series-bladder-clot-evacuation-using-a-prostate-morcellation-device
#19
Karen M Doersch, Andrew F Navetta, Erin T Bird, Marawan M El Tayeb
INTRODUCTION: We sought to provide a technical update on the use of a prostate morcellator device (PMD) to manage organized blood clots of the bladder following laser prostatectomy. METHODS: Herein, we describe our experience in using the Wolf Piranha morcellator in managing organized bladder blood clots supplemented with a retrospective chart review of the patients in whom this procedure was performed. RESULTS: Six patients, all male with a mean age of 75 ± 8...
July 2017: Canadian Urological Association Journal, Journal de L'Association des Urologues du Canada
https://www.readbyqxmd.com/read/28759964/extracorporeal-perfusion-reduced-to-a-one-way-infusion
#20
Christian D Taeger, Oliver Friedrich, Raymund E Horch, Caroline Drechsler, Annika Weigand, Fabio Hopf, Carol I Geppert, Frank Münch, Torsten Birkholz, Carina Wenzel, Sebastian Geis, Lukas Prantl, Rainer Buchholz, Konstantin Präbst
BACKGROUND: Extracorporeal perfusion (EP) is moving into focus of research in reconstructive and transplantation medicine for the preservation of amputates and free tissue transplants. The idea behind EP is the reduction of ischemia-related cell damage between separation from blood circulation and reanastomosis of the transplant. Most experimental approaches are based on a complex system that moves the perfusate in a circular course. OBJECTIVE AND METHODS: In this study, we aimed to evaluate if a simple perfusion by an infusion bag filled with an electrolyte solution can provide acceptable results in terms of flow stability, oxygen supply and viability conservation for EP of a muscle transplant...
July 25, 2017: Clinical Hemorheology and Microcirculation
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