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https://www.readbyqxmd.com/read/29169176/ablation-of-atg4b-suppressed-autophagy-and-activated-ampk-for-cell-cycle-arrest-in-cancer-cells
#1
Pei-Feng Liu, Chien-Jen Hsu, Wei-Lun Tsai, Jin-Shiung Cheng, Jih-Jung Chen, I-Fei Huang, Ho-Hsing Tseng, Hsueh-Wei Chang, Chih-Wen Shu
BACKGROUND/AIMS: ATG4B is a cysteine protease required for autophagy, which is a cellular catabolic pathway involved in energy balance. ATG4B expression is elevated during tumor growth in certain types of cancer, suggesting that ATG4B is an attractive target for cancer therapy. However, little is known about the mechanisms through which ATG4B deprivation suppresses the growth of cancer cells. METHODS: Cancer cells were transfected with either siRNA against ATG4B or an expression vector encoding wild-type ATG4BWT or encoding catalytic mutant ATG4BC74A to determine cell cycle progression by propidium iodide staining or by BrdU incorporation assay using flow cytometry...
November 23, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29169149/prokaryotic-expression-and-anti-ibdv-activity-of-chicken-interleukin-18-and-interferon-%C3%AE
#2
Baifen Song, Xiaoting Li, Jinzhu Ma, Liquan Yu, Zhenyue Feng, Zhenhua Liu, Yudong Cui
Interferon-γ (IFN-γ), a cytokine produced by activated natural killer cells and T lymphocytes, is an important regulator of innate and adaptive immunity. Interleukin (IL)-18, also known as IFN-γ-inducing factor, is a cytokine that induces T and natural killer cells to produce IFN-γ. In this study, the chicken IL-18 (ChIL-18) and chicken IFN-γ (ChIFN-γ) genes were inserted into the pET28a prokaryotic expression vector, resulting in pET28a-IL-18 and pET28a-IFN-γ, respectively. These plasmids were transformed into Escherichia coli strain BL21, and the ChIL-18 and ChIFN-γ proteins were expressed and purified...
November 24, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/29168773/verification-of-non-invasive-blood-glucose-measurement-method-based-on-pulse-wave-signal-detected-by-fbg-sensor-system
#3
Shintaro Kurasawa, Shouhei Koyama, Hiroaki Ishizawa, Keisaku Fujimoto, Shun Chino
This paper describes and verifies a non-invasive blood glucose measurement method using a fiber Bragg grating (FBG) sensor system. The FBG sensor is installed on the radial artery, and the strain (pulse wave) that is propagated from the heartbeat is measured. The measured pulse wave signal was used as a collection of feature vectors for multivariate analysis aiming to determine the blood glucose level. The time axis of the pulse wave signal was normalized by two signal processing methods: the shortest-time-cut process and 1-s-normalization process...
November 23, 2017: Sensors
https://www.readbyqxmd.com/read/29168760/mesenchymal-stromal-cells-for-antineoplastic-drug-loading-and-delivery
#4
REVIEW
Francesco Petrella, Isabella Rimoldi, Stefania Rizzo, Lorenzo Spaggiari
Mesenchymal stromal cells are a population of undifferentiated multipotent adult cells possessing extensive self-renewal properties and the potential to differentiate into a variety of mesenchymal lineage cells. They express broad anti-inflammatory and immunomodulatory activity on the immune system and after transplantation can interact with the surrounding microenvironment, promoting tissue healing and regeneration. For this reason, mesenchymal stromal cells have been widely used in regenerative medicine, both in preclinical and clinical settings...
November 23, 2017: Medicines (Basel, Switzerland)
https://www.readbyqxmd.com/read/29168144/a-review-of-chronic-granulomatous-disease
#5
REVIEW
Danielle E Arnold, Jennifer R Heimall
Chronic granulomatous disease (CGD) is a primary immunodeficiency caused by defects in any of the five subunits of the NADPH oxidase complex responsible for the respiratory burst in phagocytic leukocytes. Patients with CGD are at increased risk of life-threatening infections with catalase-positive bacteria and fungi and inflammatory complications such as CGD colitis. The implementation of routine antimicrobial prophylaxis and the advent of azole antifungals has considerably improved overall survival. Nevertheless, life expectancy remains decreased compared to the general population...
November 22, 2017: Advances in Therapy
https://www.readbyqxmd.com/read/29168112/treatment-patterns-and-outcomes-in-patients-with-kras-wild-type-metastatic-colorectal-cancer-treated-in-first-line-with-bevacizumab-or-cetuximab-containing-regimens
#6
Arthur C Houts, Sarika Ogale, Nicolas Sommer, Sacha Satram-Hoang, Mark S Walker
PURPOSE: Patients with Kirsten rat sarcoma viral oncogene wild-type (KRAS WT) metastatic colorectal cancer (mCRC) treated in first line with bevacizumab (B) or cetuximab (C) plus standard chemo backbones had comparable outcomes in phase III Cancer and Leukemia Group B (CALGB) 80405. We examined comparative effectiveness of B and C regimens in real-world community settings. METHODS: This retrospective study examined progression-free survival (PFS) and OS in a US community sample of KRAS WT mCRC patients treated with first-line B (n = 254) or C (n = 146) regimens...
November 22, 2017: Journal of Gastrointestinal Cancer
https://www.readbyqxmd.com/read/29168086/increased-white-matter-metabolic-rates-in-autism-spectrum-disorder-and-schizophrenia
#7
Serge A Mitelman, Monte S Buchsbaum, Derek S Young, M Mehmet Haznedar, Eric Hollander, Lina Shihabuddin, Erin A Hazlett, Marie-Cecile Bralet
Both autism spectrum disorder (ASD) and schizophrenia are often characterized as disorders of white matter integrity. Multimodal investigations have reported elevated metabolic rates, cerebral perfusion and basal activity in various white matter regions in schizophrenia, but none of these functions has previously been studied in ASD. We used (18)fluorodeoxyglucose positron emission tomography to compare white matter metabolic rates in subjects with ASD (n = 25) to those with schizophrenia (n = 41) and healthy controls (n = 55) across a wide range of stereotaxically placed regions-of-interest...
November 22, 2017: Brain Imaging and Behavior
https://www.readbyqxmd.com/read/29168031/neural-cells-generated-from-human-induced-pluripotent-stem-cells-as-a-model-of-cns-involvement-in-mucopolysaccharidosis-type-ii
#8
Jitka Rybová, Jana Ledvinová, Jakub Sikora, Ladislav Kuchař, Robert Dobrovolný
Mucopolysaccharidosis type II (MPSII) is a rare X-linked lysosomal storage disorder caused by mutations in the iduronate-2-sulfatase (IDS) gene (IDS, Xq28). MPSII is characterized by skeletal deformities, hearing loss, airway obstruction, hepatosplenomegaly, cardiac valvular disease, and progressive neurological impairment. At the cellular level, IDS deficiency leads to lysosomal storage of glycosaminoglycans (GAGs), dominated by accumulation of dermatan and heparan sulfates. Human induced pluripotent stem cells (iPSC) represent an alternative system that complements the available MPSII murine model...
November 22, 2017: Journal of Inherited Metabolic Disease
https://www.readbyqxmd.com/read/29167987/the-geographical-vector-in-distribution-of-genetic-diversity-for-clonorchis-sinensis
#9
Daria A Solodovnik, Yulia V Tatonova, Polina V Burkovskaya
Clonorchis sinensis, the causative agent of clonorchiasis, is one of the most important parasites that inhabit countries of East and Southeast Asia. In this study, we validated the existence of a geographical vector for C. sinensis using the partial cox1 mtDNA gene, which includes a conserved region. The samples of parasite were divided into groups corresponding to three river basins, and the size of the conserved region had a strong tendency to increase from the northernmost to the southernmost samples. This indicates the availability of the geographical vector in distribution of genetic diversity...
November 22, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29167898/factors-associated-with-failure-of-adult-strabismus-20-questionnaire-scores-to-improve-following-strabismus-surgery
#10
Sarah R Hatt, David A Leske, Kemuel L Philbrick, Jonathan M Holmes
Importance: Health-related quality of life (HRQOL) typically improves following strabismus surgery. Nevertheless, for some patients, HRQOL does not improve, and reasons for this are unknown. Objective: To identify factors associated with failure of adult strabismus-20 (AS-20) HRQOL scores to improve following strabismus surgery. Design, Setting, and Participants: Prospective observational case series at the Mayo Clinic, Rochester, Minnesota, comprising 276 adults, between July 2012 and August 2016...
November 22, 2017: JAMA Ophthalmology
https://www.readbyqxmd.com/read/29167804/extracellular-vesicle-mediated-transport-of-non-coding-rnas-between-stem-cells-and-cancer-cells-implications-in-tumor-progression-and-therapeutic-resistance
#11
REVIEW
Muhammad Nawaz
Recent years have witnessed intensive progress in studying extracellular vesicles (EVs), both for understanding their basic biology and contribution to variety of diseases, biomarker discovery, and their potential as gene delivery vectors and source of innovative therapies. As such, stem cell-derived EVs have contributed significant knowledge which led to the development of cell-free therapies in regenerative medicine. Although, the role of stem cell-derived EVs in maintaining stemness, differentiation and repairing tissue injuries is relatively well-understood; however, knowledge about the contribution of stem cell-derived EVs in cancer progression is just emerging...
2017: Stem Cell Investigation
https://www.readbyqxmd.com/read/29167655/an-individual-finger-gesture-recognition-system-based-on-motion-intent-analysis-using-mechanomyogram-signal
#12
Huijun Ding, Qing He, Yongjin Zhou, Guo Dan, Song Cui
Motion-intent-based finger gesture recognition systems are crucial for many applications such as prosthesis control, sign language recognition, wearable rehabilitation system, and human-computer interaction. In this article, a motion-intent-based finger gesture recognition system is designed to correctly identify the tapping of every finger for the first time. Two auto-event annotation algorithms are firstly applied and evaluated for detecting the finger tapping frame. Based on the truncated signals, the Wavelet packet transform (WPT) coefficients are calculated and compressed as the features, followed by a feature selection method that is able to improve the performance by optimizing the feature set...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/29167627/the-importance-of-human-population-characteristics-in-modeling-aedes-aegypti-distributions-and-assessing-risk-of-mosquito-borne-infectious-diseases
#13
Julie F Obenauer, T Andrew Joyner, Joseph B Harris
Background: The mosquito Aedes aegypti has long been a vector for human illness in the Southeastern United States. In the past, it has been responsible for outbreaks of dengue, chikungunya, and yellow fever and, very recently, the Zika virus that has been introduced to the region. Multiple studies have modeled the geographic distribution of Ae. aegypti as a function of climate factors; however, this ignores the importance of humans to the anthropophilic biter. Furthermore, Ae. aegypti thrives in areas where humans have created standing water sites, such as water storage containers and trash...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29167626/exploring-spatial-and-temporal-patterns-of-visceral-leishmaniasis-in-endemic-areas-of-bangladesh
#14
Ashraf Dewan, Abu Yousuf Md Abdullah, Md Rakibul Islam Shogib, Razimul Karim, Md Masudur Rahman
Background: Visceral leishmaniasis is a considerable public health burden on the Indian subcontinent. The disease is highly endemic in the north-central part of Bangladesh, affecting the poorest and most marginalized communities. Despite the fact that visceral leishmaniasis (VL) results in mortality, severe morbidity, and socioeconomic stress in the region, the spatiotemporal dynamics of the disease have largely remained unexplored, especially in Bangladesh. Methods: Monthly VL cases between 2010 and 2014, obtained from subdistrict hospitals, were studied in this work...
2017: Tropical Medicine and Health
https://www.readbyqxmd.com/read/29167591/identifying-hiv-associated-neurocognitive-disorder-using-large-scale-granger-causality-analysis-on-resting-state-functional-mri
#15
Adora M DSouza, Anas Z Abidin, Lutz Leistritz, Axel Wismüller
We investigate the applicability of large-scale Granger Causality (lsGC) for extracting a measure of multivariate information flow between pairs of regional brain activities from resting-state functional MRI (fMRI) and test the effectiveness of these measures for predicting a disease state. Such pairwise multivariate measures of interaction provide high-dimensional representations of connectivity profiles for each subject and are used in a machine learning task to distinguish between healthy controls and individuals presenting with symptoms of HIV Associated Neurocognitive Disorder (HAND)...
February 2017: Proceedings of SPIE
https://www.readbyqxmd.com/read/29167586/promoting-pluralism-in-counselling-an-untapped-source-of-relational-mapping-as-therapeutic-process
#16
Donna Carlyle
This paper discusses the merits of pluralism in practice. It argues for a wider recognition of creative and integrative approaches, such as those used in the field of children's geographies (involving places and spaces), as a way of unlocking practitioner potential and innovation. By re-thinking child and human development, viewing it as socially, culturally and philosophically bound, through the proposed concept of 'vectors of entanglements', the author seeks to demonstrate and encourage the application of hybrid approaches across multi-disciplinary fields...
2017: International Journal for the Advancement of Counseling
https://www.readbyqxmd.com/read/29167493/functional-aspects-of-salivary-nitric-oxide-synthase-of-rhodnius-prolixus-hemiptera-reduviidae-and-nitric-oxide-trafficking-at-the-vector-host-interface
#17
Rafaela M M Paim, Bruno W L Nascimento, Ana Mércia D Nascimento, Dimitri E Pacheco, Adriana C Soares, Ricardo N Araujo, Mauricio R V Sant'Anna, Grasielle C D Pessoa, Nelder F Gontijo, Marcos H Pereira
Rhodnius prolixus expresses nitric oxide synthase (NOS) in the cytosol of the salivary gland (SG) cells. The NO produced is stored in the SG lumen bound to NO-carrier haemeproteins called nitrophorins (NPs). NPs bind tightly to NO in the acidic SG lumen, but release NO when the pH becomes high, e.g., at the host skin (pH~7.4). NO elicits potent and transient relaxation of vascular smooth muscle. Here, we investigated the role of salivary NO in the R. prolixus feeding behaviour and the salivary vasodilator activity of the host microcirculation...
November 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29167335/%C3%AE-d-n-4-hydroxycytidine-is-a-potent-anti-alphavirus-compound-that-induces-high-level-of-mutations-in-viral-genome
#18
Nadya Urakova, Valeriya Kuznetsova, David K Crossman, Arpine Sokratian, David B Guthrie, Alexander A Kolykhalov, Mark A Lockwood, Michael G Natchus, Michael R Crowley, George R Painter, Elena I Frolova, Ilya Frolov
Venezuelan equine encephalitis virus (VEEV) is a representative member of the New World alphaviruses. It is transmitted by mosquito vectors and causes highly debilitating disease in humans, equides and other vertebrate hosts. Despite a continuous public health threat, very few compounds with anti-VEEV activity in cell culture and in mouse models have been identified to date, and rapid development of virus resistance to some of them has been recorded. In this study, we investigated the possibility of using a modified nucleoside analog, β-D-N(4)-hydroxycytidine (NHC), as an anti-VEEV agent and defined the mechanism of its anti-VEEV activity...
November 22, 2017: Journal of Virology
https://www.readbyqxmd.com/read/29167157/bipolar-distributions-in-vascular-plants-a-review
#19
Tamara Villaverde, Marcial Escudero, Santiago Martín-Bravo, Pedro Jiménez-Mejías, Isabel Sanmartín, Pablo Vargas, Modesto Luceño
Bipolar disjunct distributions are a fascinating biogeographic pattern exhibited by about 30 vascular plants, whose populations reach very high latitudes in the northern and southern hemispheres. In this review, we first propose a new framework for the definition of bipolar disjunctions and then reformulate a list of guiding principles to consider how to study bipolar species. Vicariance and convergent evolution hypotheses have been argued to explain the origin of this fragmented distribution pattern, but we show here that they can be rejected for all bipolar species, except for Carex microglochin Instead, human introduction and dispersal (either direct or by mountain-hopping)-facilitated by standard and nonstandard vectors-are the most likely explanations for the origin of bipolar plant disjunctions...
November 22, 2017: American Journal of Botany
https://www.readbyqxmd.com/read/29167115/epigenetic-modulation-of-intestinal-na-h-exchanger-3-expression
#20
Anoop Kumar, Pooja Malhotra, Hayley P Coffing, Shubha Priyamvada, Arivarasu Natarajan Anbazhagan, Harish R Krishnan, Ravinder K Gill, Waddah A Alrefai, David P Gavin, Subhash C Pandey, Pradeep K Dudeja, Seema Saksena
Na(+)/H(+) exchanger-3 (NHE3) is crucial for intestinal Na+ absorption and its reduction has been implicated in infectious and Inflammatory Bowel Diseases (IBD)-associated diarrhea. Epigenetic mechanisms such as DNA methylation are involved in the pathophysiology of IBD. Whether changes in DNA methylation are involved in modulating intestinal NHE3 gene expression is not known. Caco-2 and HUTU-80 cells were used as models of human intestinal epithelial cells (IECs). Normal C57/BL6, wild type or GADD45b KO mice were used as in vivo models...
November 22, 2017: American Journal of Physiology. Gastrointestinal and Liver Physiology
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