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https://www.readbyqxmd.com/read/28927069/overexpression-of-reic-dkk-3-suppresses-the-expression-of-cd147-and-inhibits-the-proliferation-of-human-bladder-cancer-cells
#1
Yuhei Horikawa, Masami Watanabe, Takuya Sadahira, Yuichi Ariyoshi, Yasuyuki Kobayashi, Motoo Araki, Koichiro Wada, Kazuhiko Ochiai, Shun-Ai Li, Yasutomo Nasu
Our group previously developed an adenoviral vector encoding the REIC/Dkk-3 gene (Ad-REIC), a tumor suppressor, for cancer gene therapy. The Ad-REIC agent induces apoptosis and inhibits invasion in a number of cancer cell lines; however, the molecular mechanisms underlying its effects remain unclear. Cluster of differentiation 147 (CD147), also known as extracellular matrix metalloproteinase inducer (EMMPRIN), is a key molecule that promotes cancer proliferation and invasion. In order to elucidate the therapeutic mechanism of Ad-REIC, its effect on the expression of CD147 in human bladder cancer KK47 cells was investigated...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927029/differences-in-zbtb7a-expression-cause-heterogeneous-changes-in-human-nasopharyngeal-carcinoma-cne3-sublines
#2
Fei Liu, Jiao Lan, Wei Jiao, Xianglan Mo, Yongta Huang, Huilan Ye, Ruiping Xiao, Yongli Wang, Mingzheng Mo, Liwei Shi
The present study aimed to determine the association between changes in Zbtb7a expression levels and heterogeneity of nasopharyngeal carcinoma (NPC) CNE3 sublines. CNE3 sublines were established by screening of serial dilution and continuous passage. Proliferative ability and tumorigenicity of the sublines were analyzed separately by soft-agar colony formation and mouse studies. The NPC tissues from mice were analyzed by histological evaluation and immunohistochemistry. The expression levels of Zbtb7a mRNA and protein were analyzed separately by quantitative reverse transcription polymerase chain reaction and western blotting...
September 2017: Oncology Letters
https://www.readbyqxmd.com/read/28927017/an-improved-evidential-iowa-sensor-data-fusion-approach-in-fault-diagnosis
#3
Yongchuan Tang, Deyun Zhou, Miaoyan Zhuang, Xueyi Fang, Chunhe Xie
As an important tool of information fusion, Dempster-Shafer evidence theory is widely applied in handling the uncertain information in fault diagnosis. However, an incorrect result may be obtained if the combined evidence is highly conflicting, which may leads to failure in locating the fault. To deal with the problem, an improved evidential-Induced Ordered Weighted Averaging (IOWA) sensor data fusion approach is proposed in the frame of Dempster-Shafer evidence theory. In the new method, the IOWA operator is used to determine the weight of different sensor data source, while determining the parameter of the IOWA, both the distance of evidence and the belief entropy are taken into consideration...
September 18, 2017: Sensors
https://www.readbyqxmd.com/read/28926997/effects-of-cellular-methylation-on-transgene-expression-and-site-specific-integration-of-adeno-associated-virus
#4
Diptiman Chanda, Jonathan A Hensel, Jerome T Higgs, Rajat Grover, Niroop Kaza, Selvarangan Ponnazhagan
DNA methylation is a major epigenetic event that affects not only cellular gene expression but that also has the potential to influence bacterial and viral DNA in their host-dependent functions. Adeno-associated virus (AAV) genome contains a high degree of CpG sequences capable of methylation in its terminal repeat sequences, which are the sole elements retained in AAV-based vectors used in gene therapy. The present study determined the influence of methylation status of the host cell on wild type (wt) AAV integration and recombinant (r) AAV transgene expression in HeLa cells...
September 18, 2017: Genes
https://www.readbyqxmd.com/read/28926985/linguistic-multi-attribute-group-decision-making-with-risk-preferences-and-its-use-in-low-carbon-tourism-destination-selection
#5
Hui Lin, Zhou-Jing Wang
Low-carbon tourism plays an important role in carbon emission reduction and environmental protection. Low-carbon tourism destination selection often involves multiple conflicting and incommensurate attributes or criteria and can be modelled as a multi-attribute decision-making problem. This paper develops a framework to solve multi-attribute group decision-making problems, where attribute evaluation values are provided as linguistic terms and the attribute weight information is incomplete. In order to obtain a group risk preference captured by a linguistic term set with triangular fuzzy semantic information, a nonlinear programming model is established on the basis of individual risk preferences...
September 17, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28926953/short-circuit-fault-detection-and-classification-using-empirical-wavelet-transform-and-local-energy-for-electric-transmission-line
#6
Nantian Huang, Jiajin Qi, Fuqing Li, Dongfeng Yang, Guowei Cai, Guilin Huang, Jian Zheng, Zhenxin Li
In order to improve the classification accuracy of recognizing short-circuit faults in electric transmission lines, a novel detection and diagnosis method based on empirical wavelet transform (EWT) and local energy (LE) is proposed. First, EWT is used to deal with the original short-circuit fault signals from photoelectric voltage transformers, before the amplitude modulated-frequency modulated (AM-FM) mode with a compactly supported Fourier spectrum is extracted. Subsequently, the fault occurrence time is detected according to the modulus maxima of intrinsic mode function (IMF₂) from three-phase voltage signals processed by EWT...
September 16, 2017: Sensors
https://www.readbyqxmd.com/read/28926814/one-year-persistence-of-individual-gait-patterns-identified-in-a-follow-up-study-a-call-for-individualised-diagnose-and-therapy
#7
F Horst, M Mildner, W I Schöllhorn
Although a hunch about the individuality of human movements generally exists, differences in gait patterns between individuals are often neglected. To date, only a few studies distinguished individual gait patterns in terms of uniqueness and emphasised the relevance of individualised diagnoses and therapy. However, small sample sizes have been a limitation on identifying subjects based on gait patterns, and little is known about the permanence of subject-specific characteristics over time. The purpose of this study was (1) to prove the uniqueness of individual gait patterns within a larger sample and (2) to prove the long-term permanence of individual gait patterns...
September 6, 2017: Gait & Posture
https://www.readbyqxmd.com/read/28926632/risk-analysis-of-inter-species-reassortment-through-a-rift-valley-fever-phlebovirus-mp-12-vaccine-strain
#8
Hoai J Ly, Nandadeva Lokugamage, Shoko Nishiyama, Tetsuro Ikegami
Rift Valley fever (RVF) is a mosquito-borne zoonotic disease endemic to Africa and the Arabian Peninsula. The causative agent, Rift Valley fever phlebovirus (RVFV), belongs to the genus Phlebovirus in the family Phenuiviridae and causes high rates of abortions in ruminants, and hemorrhagic fever, encephalitis, or blindness in humans. Viral maintenance by mosquito vectors has led to sporadic RVF outbreaks in ruminants and humans in endemic countries, and effective vaccination of animals and humans may minimize the impact of this disease...
2017: PloS One
https://www.readbyqxmd.com/read/28926629/learning-linear-transformations-between-counting-based-and-prediction-based-word-embeddings
#9
Danushka Bollegala, Kohei Hayashi, Ken-Ichi Kawarabayashi
Despite the growing interest in prediction-based word embedding learning methods, it remains unclear as to how the vector spaces learnt by the prediction-based methods differ from that of the counting-based methods, or whether one can be transformed into the other. To study the relationship between counting-based and prediction-based embeddings, we propose a method for learning a linear transformation between two given sets of word embeddings. Our proposal contributes to the word embedding learning research in three ways: (a) we propose an efficient method to learn a linear transformation between two sets of word embeddings, (b) using the transformation learnt in (a), we empirically show that it is possible to predict distributed word embeddings for novel unseen words, and...
2017: PloS One
https://www.readbyqxmd.com/read/28926581/imaging-grafted-cells-with-18f-fhbg-using-an-optimized-hsv1-tk-mammalian-expression-vector-in-a-brain-injury-rodent-model
#10
Anne-Sophie Salabert, Laurence Vaysse, Marie Beaurain, Mathieu Alonso, Germain Arribarat, Jean-Albert Lotterie, Isabelle Loubinoux, Mathieu Tafani, Pierre Payoux
INTRODUCTION: Cell transplantation is an innovative therapeutic approach after brain injury to compensate for tissue damage. To have real-time longitudinal monitoring of intracerebrally grafted cells, we explored the feasibility of a molecular imaging approach using thymidine kinase HSV1-TK gene encoding and [18F]FHBG as a reporter probe to image enzyme expression. METHODS: A stable neuronal cell line expressing HSV1-TK was developed with an optimised mammalian expression vector to ensure long-term transgene expression...
2017: PloS One
https://www.readbyqxmd.com/read/28926357/first-in-human-treatment-with-a-dendritic-cell-targeting-lentiviral-vector-expressing-ny-eso-1-lv305-induces-deep-durable-response-in-refractory-metastatic-synovial-sarcoma-patient
#11
Seth M Pollack, Hailing Lu, Sacha Gnjatic, Neeta Somaiah, Ryan B O'Malley, Robin L Jones, Frank J Hsu, Jan Ter Meulen
Effective induction of antitumor T cells is a pivotal goal of cancer immunotherapy. To this end, lentiviral vectors (LV) are uniquely poised to directly prime CD8 T-cell responses via transduction of dendritic cells in vivo and have shown promise as active cancer therapeutics in preclinical tumor models. However, until now, significant barriers related to production and regulation have prevented their widespread use in the clinic. We developed LV305, a dendritic cell-targeting, integration-deficient, replication incompetent LV from the ZVex platform, encoding the full-length cancer-testis antigen NY-ESO-1...
September 18, 2017: Journal of Immunotherapy
https://www.readbyqxmd.com/read/28926163/radiomics-signature-a-potential-biomarker-for-the-prediction-of-mgmt-promoter-methylation-in-glioblastoma
#12
Yi-Bin Xi, Fan Guo, Zi-Liang Xu, Chen Li, Wei Wei, Ping Tian, Ting-Ting Liu, Lin Liu, Gang Chen, Jing Ye, Guang Cheng, Long-Biao Cui, Hong-Juan Zhang, Wei Qin, Hong Yin
BACKGROUND: In glioblastoma (GBM), promoter methylation of the DNA repair gene O-methylguanine-DNA methyltransferase (MGMT) is associated with beneficial chemotherapy. PURPOSE/HYPOTHESIS: To analyze radiomics features for utilizing the full potential of medical imaging as biomarkers of MGMT promoter methylation. STUDY TYPE: Retrospective. POPULATION/SUBJECTS: In all, 98 GBM patients with known MGMT (48 methylated and 50 unmethylated tumors)...
September 19, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28926155/idna-screening-disease-vectors-as-vertebrate-samplers
#13
Arthur Kocher, Benoit De Thoisy, François Catzeflis, Sophie Valiere, Anne-Laure Bañuls, Jérôme Murienne
In the current context of global change and human-induced biodiversity decline, there is an urgent need for developing sampling approaches able to accurately describe the state of biodiversity. Traditional surveys of vertebrate fauna involve time-consuming and skill-demanding field methods. Recently, the use of DNA derived from invertebrate parasites (leeches and blowflies) was suggested as a new tool for vertebrate diversity assessment. Blood meal analyses of arthropod disease vectors have long been performed to describe their feeding behaviour, for epidemiological purposes...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28926023/the-political-economy-of-bovine-tuberculosis-in-great-britain
#14
R M Bennett
A brief history of bovine tuberculosis (bTB) and its control in Great Britain (GB) is presented. Numerous diverse policies to control the disease in humans, cattle and wildlife have been pursued over the last 100 years and many millions of pounds have been spent. After notable success in reducing the incidence and prevalence of bTB in cattle in GB from the 1950s to the mid-1980s, the geographical spread of the disease and the number of cattle slaughtered have increased continually since that time, with a high point of bTB incidence in 2008...
April 2017: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/28925998/a-critical-period-for-antidepressant-induced-acceleration-of-neuronal-maturation-in-adult-dentate-gyrus
#15
I Åmellem, S Suresh, C C Chang, S S L Tok, A Tashiro
Selective serotonin reuptake inhibitors (SSRIs) are the most commonly used medications for mood and anxiety disorders, and adult neurogenesis in the dentate gyrus has been shown to be involved in the behavioral effects of SSRIs in mice. Studies have shown the varied effects of chronic treatment with SSRIs on adult neurogenesis. One such effect is the acceleration of neuronal maturation, which affects the functional integration of new neurons into existing neuronal circuitry. In this study, we labeled new neurons by using GFP-expressing retroviral vectors in mice and investigated the effect of an SSRI, fluoxetine, on these neurons at different time points after neuronal birth...
September 19, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28925970/current-status-of-rift-valley-fever-vaccine-development
#16
REVIEW
Bonto Faburay, Angelle Desiree LaBeaud, D Scott McVey, William C Wilson, Juergen A Richt
Rift Valley Fever (RVF) is a mosquito-borne zoonotic disease that presents a substantial threat to human and public health. It is caused by Rift Valley fever phlebovirus (RVFV), which belongs to the genus Phlebovirus and the family Phenuiviridae within the order Bunyavirales. The wide distribution of competent vectors in non-endemic areas coupled with global climate change poses a significant threat of the transboundary spread of RVFV. In the last decade, an improved understanding of the molecular biology of RVFV has facilitated significant progress in the development of novel vaccines, including DIVA (differentiating infected from vaccinated animals) vaccines...
September 19, 2017: Vaccines
https://www.readbyqxmd.com/read/28925817/using-multiple-classifiers-for-predicting-the-risk-of-endovascular-aortic-aneurysm-repair-re-intervention-through-hybrid-feature-selection
#17
Omneya Attallah, Alan Karthikesalingam, Peter Je Holt, Matthew M Thompson, Rob Sayers, Matthew J Bown, Eddie C Choke, Xianghong Ma
Feature selection is essential in medical area; however, its process becomes complicated with the presence of censoring which is the unique character of survival analysis. Most survival feature selection methods are based on Cox's proportional hazard model, though machine learning classifiers are preferred. They are less employed in survival analysis due to censoring which prevents them from directly being used to survival data. Among the few work that employed machine learning classifiers, partial logistic artificial neural network with auto-relevance determination is a well-known method that deals with censoring and perform feature selection for survival data...
September 1, 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28925816/investigating-the-constrained-action-hypothesis-a-movement-coordination-and-coordination-variability-approach
#18
Anthony Vidal, Will Wu, Mimi Nakajima, James Becker
The purpose of this study was to examine the effects of focus of attention cues on movement coordination and coordination variability in the lower extremity. Twenty participants performed the standing long jump under both internal and external focus of attention conditions. A modified vector coding technique was used to evaluate the influence of attentional focus cues on lower extremity coordination patterns and coordination variability during the jumps. Participants jumped significantly further under an external focus of attention condition compared with an internal focus of attention condition (p = ...
September 19, 2017: Journal of Motor Behavior
https://www.readbyqxmd.com/read/28925795/application-of-replication-defective-west-nile-virus-vector-to-non-flavivirus-vaccine-targets
#19
Maryann Giel-Moloney, Michael Vaine, Linong Zhang, Mark Parrington, Beata Gajewska, Thorsten U Vogel, Svetlana O Pougatcheva, Xiaochu Duan, Timothy Farrell, Irina Ustyugova, Sanjay Phogat, Harry Kleanthous, Konstantin V Pugachev
The RepliVaxvaccine platform(RV) is based on flavivirus genomes that are rationally attenuated by deletion. The self-limiting infection provided by RV has been demonstrated to be safe, highly immunogenic and efficacious for several vaccine candidates against flaviviruses. Here respiratory syncytial virus (RSV) F, influenza virus HA, and Simian Immunodeficiency virus (SIV) Env proteins were expressed in place of either prM-E or C-prM-E gene deletions of the West Nile (WN) genome. The resulting RV-RSV, -influenza and -SIV vaccine prototypes replicated efficiently in complementing helper cells expressing the WN structural proteins in trans...
September 19, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28925793/sequential-administration-of-mva-based-vaccines-and-pd-1-pd-l1-blocking-antibodies-confers-measurable-benefits-on-tumor-growth-and-survival-preclinical-studies-with-mva-%C3%AE-gal-and-mva-muc1-tg4010-in-a-murine-tumor-model
#20
Christelle Remy-Ziller, Christine Thioudellet, Julie Hortelano, Murielle Gantzer, Virginie Nourtier, Marie-Christine Claudepierre, Benoit Sansas, Xavier Préville, Kaïdre Bendjama, Eric Quemeneur, Karola Rittner
TG4010, a Modified Vaccinia virus Ankara (MVA) expressing human mucin1 (MUC1) has demonstrated clinical benefit for patients suffering from advanced non-small cell lung cancer (NSCLC) in combination with chemotherapy. To support its development, preclinical experiments were performed with either TG4010 or β-galactosidase-encoding MVA vector (MVA-βgal) in mice presenting tumors in the lung. Tumor growth was obtained after intravenous injection of CT26 murine colon cancer cells, engineered to express either MUC1 or βgal...
September 19, 2017: Human Vaccines & Immunotherapeutics
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