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https://www.readbyqxmd.com/read/29779282/-effect-of-bone-morphogenetic-protein-4-overexpression-on-the-biological-activity-of-mouse-induced-pluripotent-stem-cells
#1
Jin Qiu, Hui-Yu Zhang, Li Liu, Ying-Hui Tan
OBJECTIVE: This study aimed to construct the expression of bone morphogenetic protein-4 (BMP4) lentiviral vector gene and explore its influence on the biological activity of mouse induced pluripotent stem (iPS) cells. METHODS: iPS cell lines stably overexpressing BMP4 were constructed by lentivirus transfection (BMP4-overexpressing group). Cells without transfection served as the blank group, and cells with only vector transfection served as the empty-vector group...
April 1, 2018: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/29779223/gene-editing-vectors-for-studying-nicotinic-acetylcholine-receptors-in-cholinergic-transmission
#2
Can Peng, Yijin Yan, Veronica J Kim, Staci E Engle, Jennifer N Berry, J Michael McIntosh, Rachael L Neve, Ryan M Drenan
Nicotinic acetylcholine receptors (nAChRs), prototype members of the cys-loop ligand gated ion channel family, are key mediators of cholinergic transmission in the central nervous system. Despite their importance, technical gaps exist in our ability to dissect the function of individual subunits in the brain. To overcome these barriers, we designed CRISPR/Cas9 small guide RNA sequences (sgRNAs) for production of loss-of-function alleles in mouse nAChR genes. These sgRNAs were validated in vitro via deep sequencing...
May 19, 2018: European Journal of Neuroscience
https://www.readbyqxmd.com/read/29779087/down-regulation-of-mdr1-by-ad-dkk3-via-akt-nf%C3%AE%C2%BAb-pathways-augments-the-anti-tumor-effect-of-temozolomide-in-glioblastoma-cells-and-a-murine-xenograft-model
#3
Toshitaka Fujihara, Yoshifumi Mizobuchi, Kohei Nakajima, Teruyoshi Kageji, Kazuhito Matsuzaki, Keiko T Kitazato, Ryotaro Otsuka, Keijiro Hara, Hideo Mure, Toshiyuki Okazaki, Kazuyuki Kuwayama, Shinji Nagahiro, Yasushi Takagi
BACKGROUND: Glioblastoma multiforme (GBM) is the most malignant of brain tumors. Acquired drug resistance is a major obstacle for successful treatment. Earlier studies reported that expression of the multiple drug resistance gene (MDR1) is regulated by YB-1 or NFκB via the JNK/c-Jun or Akt pathway. Over-expression of the Dickkopf (DKK) family member DKK3 by an adenovirus vector carrying DKK3 (Ad-DKK3) exerted anti-tumor effects and led to the activation of the JNK/c-Jun pathway. We investigated whether Ad-DKK3 augments the anti-tumor effect of temozolomide (TMZ) via the regulation of MDR1...
May 19, 2018: Journal of Neuro-oncology
https://www.readbyqxmd.com/read/29778975/establishment-of-an-integration-free-induced-pluripotent-stem-cell-line-musii005-a-from-exfoliated-renal-epithelial-cells
#4
Bootsakorn Boonkaew, Weeradee Thummavichit, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch
Human induced pluripotent stem cells (iPSCs) were generated from exfoliated renal epithelial cells isolated from a urine sample of a 31-year-old healthy woman. Epithelial cells were characterized for the expression of E-cadherin and reprogrammed using non-integrating Sendai viral vectors. The urine-derived iPSC line (designated as MUSIi005-A) was karyotypically normal, expressed pluripotent markers, differentiated into cells of three embryonic germ layers, and showed no viral and transgene expressions at passage 29...
May 16, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/29778904/c-lysozyme-contributes-to-antiviral-immunity-in-bombyx-mori-against-nucleopolyhedrovirus-infection
#5
Ting-Ting Chen, Li-Rong Tan, Nan Hu, Zhan-Qi Dong, Zhi-Gang Hu, Ya-Ming Jiang, Peng Chen, Min-Hui Pan, Cheng Lu
Lysozymes is a ubiquitous immune effector that is widely distributed in both vertebrates and invertebrates. Previous reports have shown that lysozymes significantly inhibit viral infections in vertebrates. However, the antiviral effects of lysozymes in invertebrates remain unclear. Here, we investigated the role of lysozymes in Bombyx mori (B. mori) response to viral infection by overexpressing B. mori C-lysozyme (BmC-LZM) in larvae and cells. We found that BmC-LZM was up-regulated in cells in response to viral infection...
May 17, 2018: Journal of Insect Physiology
https://www.readbyqxmd.com/read/29778891/leukocytes-and-drug-resistant-cancer-cells-are-targets-for-intracellular-delivery-by-adenoviral-dodecahedron
#6
Marta Jedynak, David Laurin, Patryk Dolega, Malgorzata Podsiadla-Bialoskorska, Inga Szurgot, Jadwiga Chroboczek, Ewa Szolajska
One of the major factors limiting the effectiveness of cancer chemotherapy is inefficient drug delivery. Systems enabling efficient delivery and enhanced intracellular uptake, appear particularly promising in this respect. Virus-like particle, adenoviral dodecahedron (Dd), employs receptor-mediated endocytosis for cell penetration and is able to deliver intracellularly dozens of cargo molecules attached to one particle. We focused on studying Dd properties in the context of cancer treatment, showing that intratumoral injection of Dd, assessed in mouse xenograft model, results in vector accumulation in tumor without spreading in off-target organs...
May 17, 2018: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/29778418/correlation-between-translational-and-rotational-kinematic-abnormalities-and-osteoarthritis-like-damage-in-two-in-vivo-sheep-injury-models
#7
Mehdi Shekarforoush, Jillian E Beveridge, David A Hart, Cyril B Frank, Nigel G Shrive
The relations between kinematic abnormalities and post traumatic osteoarthritis have not yet been clearly elucidated. This study was conducted to determine the finite helical axes parameters and the tibiofemoral translation vector in the knee joints of two surgically induced injury sheep models: anterior cruciate ligament and medial collateral ligament transection (ACL/MCL Tx) (n = 5) and lateral meniscectomy (n = 5). We hypothesized that morphological damage in the experimental joints would be correlated to alterations in these kinematic variables...
May 16, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29778203/evaluation-of-the-immune-response-afforded-by-a-subunit-vaccine-candidate-against-bluetongue-virus-in-mice-and-sheep
#8
Darien Kheder Ali Mohamed, Junzheng Du, Shandian Gao, Zhancheng Tian, Guorui Zhang, Dexuan Huang, Rongsheng Du, Biao Kang, Guangyuan Liu, Jianxun Luo, Hong Yin
Bluetongue virus (BTV), a vector-borne pathogen, is the causative agent of bluetongue disease in ruminants. In view of the recent emergence of BTV in regions previously known to be free from the disease and/or specific serotypes or strains, optimization of the currently available vaccination strategies to control the spread of vector-borne bluetongue is crucial. The main objective of the current study was to develop a subunit vaccine candidate targeting BTV-16, a strain previously isolated in China from sheep with obvious clinical signs...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778202/porcine-monocyte-derived-dendritic-cells-can-be-differentially-activated-by-vesicular-stomatitis-virus-and-its-matrix-protein-mutants
#9
Guoyan Sun, Xinkui Fang, Hao Wu, Xinchu Zhou, Yong Ke, Tao Sun
Vesicular stomatitis virus (VSV) can cause serious vesicular lesions in pigs, and the matrix (M) protein is its predominant virulence factor. Dendritic cells (DCs) act as the bridge between innate and adaptive immune responses. However, the susceptibility of porcine DCs to VSV infection and the role of M protein in modulating the function of infected DCs are still poorly defined. Thus, this study aimed to determine the ability of virulent wild-type VSV(wtVSV) and two attenuated M protein variants (VSVΔM51 and VSVMT ) to induce maturation of porcine monocyte-derived DCs (MoDCs) in vitro...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778186/identification-of-antigenic-epitopes-of-monoclonal-antibodies-against-the-vp2-protein-of-the-25-serotype-of-bluetongue-virus
#10
Shuangyu Xie, Yiming Shi, Ruyue Gong, Wen Cui, Yanping Jiang, Min Liu, Li Wang, Han Zhou, Xinyuan Qiao, Yijing Li, Yigang Xu, Lijie Tang
Serious outbreaks of bluetongue, an arbovirus of domestic and wild ruminants caused by bluetongue virus serotypes (BTV), have occurred around the world. More than 27 distinct serotypes are recognized throughout the world. A new virus, BTV-25 (Toggenburg orbivirus [TOV]), was first detected in Switzerland, and has not yet been found in China. VP2 is an important outer shell protein that defines BTV serotypes and is, therefore, an ideal target antigen for serotype identification. To produce a monoclonal antibody against VP2 of BTV-25, the segment 2 gene was divided into three segments, cloned into pET-28a (+) and pMAL-c5X vectors, and the protein was expressed in E...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29778179/recombinant-adenovirus-delivered-soluble-cd163-and-sialoadhesin-receptors-protected-pigs-from-porcine-reproductive-and-respiratory-syndrome-virus-infection
#11
Wenlong Xia, Zhi Wu, Changming Guo, Shanyuan Zhu, Xinyu Zhang, Xiaoli Xia, Huaichang Sun
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases affecting pig industry worldwide. Sialoadehesin (Sn) and CD163 are the two specific receptors for PRRSV infection of porcine alveolar macrophages. Our previous study showed that the soluble Sn receptor Sn4D-Fc and soluble CD163 receptor SRCR59-Fc expressed by the two recombinant adenoviral (rAd) vectors have an additive anti-PRRSV effect in vitro. In the present study, rAd-Sn4D-Fc and rAd-SRCR59-Fc were inoculated into pigs, and the efficient expression of Sn4D-Fc and SRCR59-Fc proteins was detected by ELISA...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29777946/screening-method-for-rapid-classification-of-psychoactive-substances-in-illicit-tablets-using-mid-infrared-spectroscopy-and-pls-da
#12
Leandro S A Pereira, Fernanda L C Lisboa, José Coelho Neto, Frederico N Valladão, Marcelo M Sena
Several new psychoactive substances (NPS) have reached the illegal drug market in recent years, and ecstasy-like tablets are one of the forms affected by this change. Cathinones and tryptamines have increasingly been found in ecstasy-like seized samples as well as other amphetamine type stimulants. A presumptive method for identifying different drugs in seized ecstasy tablets (n=92) using ATR-FTIR (attenuated total reflectance - Fourier transform infrared spectroscopy) and PLS-DA (partial least squares discriminant analysis) was developed...
May 9, 2018: Forensic Science International
https://www.readbyqxmd.com/read/29777716/probability-of-viral-labeling-of-neural-stem-cells-in-vivo
#13
Gregory W Kirschen, Shaoyu Ge, Il Memming Park
In the neuroscience field over the past several decades, viral vectors have become powerful gene delivery systems to study neural populations of interest. For neural stem cell (NSC) biology, such viruses are often used to birth-date and track NSCs over developmental time in lineage tracing experiments. Yet, the probability of successful infection of a given stem cell in vivo remains unknown. This information would be helpful to inform investigators interested in titrating their viruses to selectively target sparsely-populated clusters of cells in the nervous system...
May 16, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29777650/modeling-dengue-vector-population-using-remotely-sensed-data-and-machine-learning
#14
Juan M Scavuzzo, Francisco Trucco, Manuel Espinosa, Carolina B Tauro, Marcelo Abril, Carlos M Scavuzzo, Alejandro C Frery
Mosquitoes are vectors of many human diseases. In particular, Aedes ægypti (Linnaeus) is the main vector for Chikungunya, Dengue, and Zika viruses in Latin America and it represents a global threat. Public health policies that aim at combating this vector require dependable and timely information, which is usually expensive to obtain with field campaigns. For this reason, several efforts have been done to use remote sensing due to its reduced cost. The present work includes the temporal modeling of the oviposition activity (measured weekly on 50 ovitraps in a north Argentinean city) of Aedes ægypti (Linnaeus), based on time series of data extracted from operational earth observation satellite images...
May 16, 2018: Acta Tropica
https://www.readbyqxmd.com/read/29777592/early-identification-of-unusually-clustered-mutations-and-a-root-cause-in-therapeutic-antibody-development
#15
Yueming Qian, Zhiqiang Chen, Xin Huang, Xuning Wang, Xuankuo Xu, Stefan Kirov, Richard Ludwig, Nan-Xin Qian, Kandasamy Ravi, Li Tao, Michael C Borys, Zheng Jian Li
This study reports findings of an unusual cluster of mutations spanning 22 base pairs in a monoclonal antibody (mAb) expression vector. It was identified by two orthogonal methods: mass spectrometry on expressed protein and next-generation sequencing (NGS) on the plasmid DNA. While the initial NGS analysis confirmed the designed sequence modification, intact mass analysis detected an additional mass of the antibody molecule expressed in CHO cells. The extra mass was eventually found to be associated with unmatched nucleotides in a distal region by checking full-length sequence alignment plots...
May 19, 2018: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/29777580/context-encoding-enables-machine-learning-based-quantitative-photoacoustics
#16
Thomas Kirchner, Janek Gröhl, Lena Maier-Hein
Real-time monitoring of functional tissue parameters, such as local blood oxygenation, based on optical imaging could provide groundbreaking advances in the diagnosis and interventional therapy of various diseases. Although photoacoustic (PA) imaging is a modality with great potential to measure optical absorption deep inside tissue, quantification of the measurements remains a major challenge. We introduce the first machine learning-based approach to quantitative PA imaging (qPAI), which relies on learning the fluence in a voxel to deduce the corresponding optical absorption...
May 2018: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/29777505/a-fingertip-force-prediction-model-for-grasp-patterns-characterised-from-the-chaotic-behaviour-of-eeg
#17
Rinku Roy, Debdeep Sikdar, Manjunatha Mahadevappa, C S Kumar
A stable grasp is attained through appropriate hand preshaping and precise fingertip forces. Here, we have proposed a method to decode grasp patterns from motor imagery and subsequent fingertip force estimation model with a slippage avoidance strategy. We have developed a feature-based classification of electroencephalography (EEG) associated with imagination of the grasping postures. Chaotic behaviour of EEG for different grasping patterns has been utilised to capture the dynamics of associated motor activities...
May 19, 2018: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29777415/preparation-of-biologically-active-monomeric-recombinant-zebrafish-danio-rerio-and-rainbow-trout-oncorhynchus-mykiss-recombinant-growth-hormones
#18
Ewa Ocłoń, Gili Solomon, Zvi Hayouka, Arieh Gertler
Fish growth hormones (GHs) play an important role in regulating growth, metabolism, reproduction, osmoregulation, and immunity and have thus garnered attention for their application in aquaculture. Zebrafish GH (zGH) cDNA or rainbow trout GH (rtGH) cDNA was cloned into the pMon3401 vector, expressed in MON105-competent Escherichia coli and purified to homogeneity. Their biological activity was evidenced by their ability to interact with ovine GH receptor extracellular domain and stimulate GH receptor-mediated proliferation in FDC-P1-3B9 cells stably transfected with rabbit GH receptor...
May 18, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29777177/controlling-vector-borne-diseases-by-releasing-modified-mosquitoes
#19
REVIEW
Heather A Flores, Scott L O'Neill
Aedes mosquito-transmitted diseases, such as dengue, Zika and chikungunya, are becoming major global health emergencies while old threats, such as yellow fever, are re-emerging. Traditional control methods, which have focused on reducing mosquito populations through the application of insecticides or preventing breeding through removal of larval habitat, are largely ineffective, as evidenced by the increasing global disease burden. Here, we review novel mosquito population reduction and population modification approaches with a focus on control methods based on the release of mosquitoes, including the release of Wolbachia-infected mosquitoes and strategies to genetically modify the vector, that are currently under development and have the potential to contribute to a reversal of the current alarming disease trends...
May 18, 2018: Nature Reviews. Microbiology
https://www.readbyqxmd.com/read/29777147/prediction-of-moisture-content-for-congou-black-tea-withering-leaves-using-image-features-and-nonlinear-method
#20
Gaozhen Liang, Chunwang Dong, Bin Hu, Hongkai Zhu, Haibo Yuan, Yongwen Jiang, Guoshuang Hao
Withering is the first step in the processing of congou black tea. With respect to the deficiency of traditional water content detection methods, a machine vision based NDT (Non Destructive Testing) method was established to detect the moisture content of withered leaves. First, according to the time sequences using computer visual system collected visible light images of tea leaf surfaces, and color and texture characteristics are extracted through the spatial changes of colors. Then quantitative prediction models for moisture content detection of withered tea leaves was established through linear PLS (Partial Least Squares) and non-linear SVM (Support Vector Machine)...
May 18, 2018: Scientific Reports
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