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https://www.readbyqxmd.com/read/29768001/crystal-structures-of-the-mango-ii-rna-aptamer-reveal-heterogeneous-fluorophore-binding-and-guide-engineering-of-variants-with-improved-selectivity-and-brightness
#1
Robert Trachman, Amir Abdolahzadeh, Alessio Andreoni, Razvan Cojocaru, Jay R Knutson, Michaël Ryckelynck, Peter John Unrau, Adrian Ferré-D'Amaré
Several RNA aptamers that bind small molecules and enhance their fluorescence have been successfully used to tag and track RNAs in vivo, but these genetically encodable tags have not yet achieved single-fluorophore resolution. Recently, Mango-II, an RNA that binds TO1-Biotin with ~1 nM affinity and enhances its fluorescence by >1,500-fold was isolated by fluorescence selection from the pool that yielded the original RNA Mango. We determined the crystal structures of Mango-II in complex with two fluorophores, TO1-Biotin and TO3-Biotin, and found that despite their high affinity, the ligands adopt multiple distinct conformations, indicative of a binding pocket with modest stereoselectivity...
May 16, 2018: Biochemistry
https://www.readbyqxmd.com/read/29660781/effects-of-site-and-cultivar-on-consumer-acceptance-of-pomegranate
#2
John M Chater, Donald J Merhaut, Zhenyu Jia, Mary Lu Arpaia, Peggy A Mauk, John E Preece
Pomegranate (Punica granatum L.) is an important fruit in many cultures. The fruit and juice have risen in popularity as it was discovered that pomegranate has relatively high antioxidant activity compared to most other fruits. In this study, six cultivars were utilized to determine consumer acceptance compared to the industry standard, 'Wonderful,' which comprises 90% to 95% of commercial production in the United States. Fruit were sourced from 2 cultivar field trials, one in inland Riverside, California, and one in coastal Ventura County, California...
April 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29574226/role-of-asic1a-in-a%C3%AE-induced-synaptic-alterations-in-the-hippocampus
#3
D Mango, R Nisticò
Acid-sensing ion channels (ASICs) are widely expressed in the mammalian central nervous system where they play a key role in synaptic transmission and in specific forms of memory. On the other hand, ASICs can be persistently active under pathological conditions contributing to neuronal damage in ischemic stroke, brain trauma, epilepsy and Parkinson's disease. However, to date no experimental evidence has linked ASICs to Alzheimer's disease (AD). Aim of the present work was to investigate, in CA1 pyramidal neurons, the possible involvement of ASIC1a in the Aβ-mediated effect on metabotropic glutamate (mGlu) receptor dependent transmission...
March 21, 2018: Pharmacological Research: the Official Journal of the Italian Pharmacological Society
https://www.readbyqxmd.com/read/29440634/fluorogenic-rna-mango-aptamers-for-imaging-small-non-coding-rnas-in-mammalian-cells
#4
Alexis Autour, Sunny C Y Jeng, Adam D Cawte, Amir Abdolahzadeh, Angela Galli, Shanker S S Panchapakesan, David Rueda, Michael Ryckelynck, Peter J Unrau
Despite having many key roles in cellular biology, directly imaging biologically important RNAs has been hindered by a lack of fluorescent tools equivalent to the fluorescent proteins available to study cellular proteins. Ideal RNA labelling systems must preserve biological function, have photophysical properties similar to existing fluorescent proteins, and be compatible with established live and fixed cell protein labelling strategies. Here, we report a microfluidics-based selection of three new high-affinity RNA Mango fluorogenic aptamers...
February 13, 2018: Nature Communications
https://www.readbyqxmd.com/read/29371809/development-of-a-high-resolution-multi-locus-microsatellite-typing-method-for-colletotrichum-gloeosporioides
#5
Nikita Mehta, Ferry Hagen, Sadaf Aamir, Sanjay K Singh, Abhishek Baghela
Colletotrichum gloeosporioides is an economically important fungal pathogen causing substantial yield losses indifferent host plants. To understand the genetic diversity and molecular epidemiology of this fungus, we have developed a novel, high-resolution multi-locus microsatellite typing (MLMT) method. Bioinformatic analysis of C. gloeosporioides unannotated genome sequence yielded eight potential microsatellite loci, of which five, CG1 (GT)n , CG2 (GT1)n , CG3 (TC)n , CG4 (CT)n , and CG5 (CT1)n were selected for further study based on their universal amplification potential, reproducibility, and repeat number polymorphism...
December 2017: Mycobiology
https://www.readbyqxmd.com/read/29241317/optimization-of-soymilk-mango-nectar-and-sucrose-solution-mixes-for-better-quality-of-soymilk-based-beverage
#6
Rahel Getu, Yetenayet B Tola, Satheesh Neela
BACKGROUND: Soy milk-based beverages play an important role as a healthy food alternative for human consumption. However, the ‘beany’ flavor and chalky mouth feel of soy milk often makes it unpalatable to consumers. The objective of the present study is to optimize a blend of soy milk, mango nectar and sucrose solution for the best quality soy milk-based beverage. METHODS: This study was designed to develop a soy milk blended beverage, with mango nectar and sucrose solutions, with the best physicochemical and sensory properties...
October 2017: Acta Scientiarum Polonorum. Technologia Alimentaria
https://www.readbyqxmd.com/read/29184254/characterization-of-probiotic-potential-of-bacillus-species-isolated-from-a-traditional-brine-pickle
#7
K Ragul, Ibadondor Syiem, K Sundar, Prathapkumar H Shetty
Fermented foods are known for their potential as main source of probiotics. The present study aimed at investigating the probiotic properties of bacteria isolated from fermented mango pickle. Non-hemolytic fermenting microbiota isolated from mango pickle was screened in vitro for their basic probiotic properties such as acid tolerance, bile salt, lysozyme and salt tolerance. They were also tested for their beneficial characters like cholesterol removal, bacterial adhesion to hydrocarbons, auto-aggregation, antimicrobial activity, β-galactosidase activity, exopolysaccharide production and adhesion to HT-29 cell line...
December 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29174226/optical-coherence-tomography-a-novel-imaging-method-for-post-lumpectomy-breast-margin-assessment-a-multi-reader-study
#8
Richard Ha, Lauren C Friedlander, Hanina Hibshoosh, Christine Hendon, Sheldon Feldman, Soojin Ahn, Hank Schmidt, Margaret K Akens, MaryAnn Fitzmaurice, Brian C Wilson, Victoria L Mango
RATIONALE AND OBJECTIVES: This study aimed to assess whether different breast cancer subspecialty physicians can be trained to distinguish non-suspicious from suspicious areas of post-lumpectomy specimen margin in patients with breast cancer using optical coherence tomography (OCT) images (a near-infrared based imaging technique) with final histology as the reference standard. MATERIALS AND METHODS: This institutional review board-exempt, Health Insurance Portability and Accountability Act-compliant study was performed on 63 surgically excised breast specimens from 35 female patients, creating a 90-case atlas containing both non-suspicious and suspicious areas for cancer...
November 23, 2017: Academic Radiology
https://www.readbyqxmd.com/read/29097776/misnpdb-a-web-based-genomic-resources-of-tropical-ecology-fruit-mango-mangifera-indica-l-for-phylogeography-and-varietal-differentiation
#9
M A Iquebal, Sarika Jaiswal, Ajay Kumar Mahato, Pawan K Jayaswal, U B Angadi, Neeraj Kumar, Nimisha Sharma, Anand K Singh, Manish Srivastav, Jai Prakash, S K Singh, Kasim Khan, Rupesh K Mishra, Shailendra Rajan, Anju Bajpai, B S Sandhya, Puttaraju Nischita, K V Ravishankar, M R Dinesh, Anil Rai, Dinesh Kumar, Tilak R Sharma, Nagendra K Singh
Mango is one of the most important fruits of tropical ecological region of the world, well known for its nutritive value, aroma and taste. Its world production is >45MT worth >200 billion US dollars. Genomic resources are required for improvement in productivity and management of mango germplasm. There is no web-based genomic resources available for mango. Hence rapid and cost-effective high throughput putative marker discovery is required to develop such resources. RAD-based marker discovery can cater this urgent need till whole genome sequence of mango becomes available...
November 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29063625/effect-of-phenolic-compounds-on-the-growth-of-selected-probiotic-and-pathogenic-bacteria
#10
R Pacheco-Ordaz, A Wall-Medrano, M G Goñi, G Ramos-Clamont-Montfort, J F Ayala-Zavala, G A González-Aguilar
Fruit extracts from different tissues (pulp, seed and peel) have shown antimicrobial and prebiotic activities related to their phenolic profile, although structure-specific evaluations have not been reported yet. The effect of five phenolic compounds (catechin and gallic, vanillic, ferulic and protocatechuic acids) identified in different fruits, particularly in mango, was evaluated on the growth of two probiotic (Lactobacillus rhamnosusGG ATCC 53103 and Lactobacillus acidophilusNRRLB 4495) and two pathogenic (Escherichia coli 0157:H7 ATCC 43890 and Salmonella enterica serovar Typhimurium ATCC 14028) bacteria...
January 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28974096/quantitation-of-minor-components-in-mango-juice-with-band-selective-excitation-nmr-spectroscopy
#11
Shoraku Ryu, Masanori Koda, Takuya Miyakawa, Masaru Tanokura
1 H NMR-based metabolic analysis of foods has been widely applied. However, dynamic range problems frequently impede its application because foodstuffs are composed of various organic compounds in a wide range of concentrations. Band-selective excitation1 H NMR spectroscopy has been found to be a useful tool for observing the minor components in foods. Because quantitative information is important for metabolic analysis of foods and complete metabolome data, quantitation with the band-selective excitation1 H NMR method was carefully investigated in the present study...
November 1, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28932849/new-insights-into-single-compound-and-binary-adsorption-of-copper-and-lead-ions-on-a-treated-sea-mango-shell-experimental-and-theoretical-studies
#12
Lotfi Sellaoui, Felycia Edi Soetaredjo, Suryadi Ismadji, Éder Cláudio Lima, Guilherme L Dotto, Abdelmottaleb Ben Lamine, Alessandro Erto
Herein, adsorption isotherms of Pb(ii) and Cu(ii) ions on treated sea mango fruit in both single-compound and binary systems were experimentally realized at different temperatures in the range of 30-50 °C. Experimental results show that adsorption of Pb(ii) was more as compared to that of Cu(ii) ions; however, for both ions, a significant reduction in the adsorption capacity was observed in the binary system as compared to that in the single-compound systems. Moreover, under all the investigated conditions, adsorption seems to be promoted by an increase in temperature...
October 4, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28891568/selective-antitumoural-action-of-pressurized-mango-leaf-extracts-against-minimally-and-highly-invasive-breast-cancer
#13
M T Fernández-Ponce, A López-Biedma, C Sánchez-Quesada, L Casas, C Mantell, J J Gaforio, E J Martínez de la Ossa
Mango leaf tea has been traditionally used by different cultures to reduce inflammation in the body. There is evidence that chronic inflammation increases the risk of cancer. This study investigates the antitumoural effects of pressurized mango leaf extracts on minimally (MCF7) and highly invasive (MDA-MB-231) breast cancer cells as well as on non-tumourigenic cells (MCF10). Extracts showed protective properties against oxidation and cytotoxic effects against breast cancer cell lines, causing minor damage to non-carcinogenic cells...
October 18, 2017: Food & Function
https://www.readbyqxmd.com/read/28821039/processes-involving-selective-precipitation-for-the-recovery-of-purified-pectins-from-mango-peel
#14
Andreas Nagel, Carina Winkler, Reinhold Carle, Hans-Ulrich Endress, Christine Rentschler, Sybille Neidhart
Three methods for the recovery of purified pectins from directly dried mango peel were developed, using selective precipitation of mango pectin in propan-2-ol (IPA) of adequate volume concentrations for purification. Yields, composition, macromolecular and gelling properties of the resultant pectins were compared. Effluent analyses proved postextractive removal of fruit exudate arabinogalactans. The recovery processes involved (A) washing of raw-pectin powder in IPA of defined volume concentration, (B) fractional alcoholic precipitation of dissolved raw pectin, or (C) selective pectin precipitation from the hot-acid extract of mango peel in adequately diluted IPA...
October 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28763996/a-novel-magnetic-ion-imprinted-polymer-as-a-selective-magnetic-solid-phase-for-separation-of-trace-lead-ii-ions-from-agricultural-products-and-optimization-using-a-box-behnken-design
#15
Zohreh Dahaghin, Hassan Zavvar Mousavi, S Maryam Sajjadi
In this work, a magnetic ion-imprinted polymer (Fe3O4@SiO2@IIP) as a novel and selective nanosorbent for selective extraction of Pb(II) ions from various agricultural products is presented. The novel lead magnetic ion-imprinted polymer was synthesized by imidazole as a new ligand and grafted onto the surface of Fe3O4@SiO2 NPs. A Box-Behnken (BBD) design was used for optimization of the extraction and elution steps. In the selected conditions, the limit of detection was 0.48ngmL(-1), preconcentration factor was 300, the sorption capacity of this new magnetic ion-imprinted polymer was 105mgg(-1), and the precision of the method (RSD%) for six replicate measurements was found 3...
December 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28737267/appearance-and-characterization-of-fruit-image-textures-for-quality-sorting-using-wavelet-transform-and-genetic-algorithms
#16
Suchitra Khoje
Images of four qualities of mangoes and guavas are evaluated for color and textural features to characterize and classify them, and to model the fruit appearance grading. The paper discusses three approaches to identify most discriminating texture features of both the fruits. In the first approach, fruit's color and texture features are selected using Mahalanobis distance. A total of 20 color features and 40 textural features are extracted for analysis. Using Mahalanobis distance and feature intercorrelation analyses, one best color feature (mean of a* [L*a*b* color space]) and two textural features (energy a*, contrast of H*) are selected as features for Guava while two best color features (R std, H std) and one textural features (energy b*) are selected as features for mangoes with the highest discriminate power...
February 2018: Journal of Texture Studies
https://www.readbyqxmd.com/read/28578060/the-impact-of-fruit-and-soybean-by-products-and-amaranth-on-the-growth-of-probiotic-and-starter-microorganisms
#17
Antonio Diogo Silva Vieira, Raquel Bedani, M A C Albuquerque, Vanessa Biscola, Susana Marta Isay Saad
The ability of different fruit by-products, okara, and amaranth flour, to support the growth of probiotic and non-probiotic strains was evaluated. The tests were conducted with three commercial starter cultures (Streptococcus thermophilus), ten probiotic strains (seven Lactobacillus spp. and three Bifidobacterium spp. strains), and two harmful bacteria representative of the intestinal microbiota (Escherichia coli and Clostridium perfringens). In vitro fermentability assays were performed using a modified MRS broth supplemented with different fruits (acerola, orange, passion fruit, and mango), and soy (okara) by-products or amaranth flour...
July 2017: Food Research International
https://www.readbyqxmd.com/read/28577516/improvement-of-bacillus-strains-by-mutation-for-overproduction-of-exopolygalacturonases
#18
Hira Muzzamal, Zakia Latif
Pectinases, produced by microorganisms, have wide range application in food industry, textile processing, paper making, coffee and tea fermentation, etc. It accounts for 10% of the global industrial enzymes produced. The most important and widely used commercial pectinase polygalacturonase is produced by alkalophilic strains of Bacillus sp. and Streptomyces sp. Here, we explored 29 bacterial strains isolated from rotten mango samples for polygalacturonase production and selected 16 strains through preliminary screening by well-plate method for enzyme activity...
August 2016: Indian Journal of Experimental Biology
https://www.readbyqxmd.com/read/28551261/production-of-sn-1-3-distearoyl-2-oleoyl-glycerol-rich-fats-from-mango-kernel-fat-by-selective-fractionation-using-2-methylpentane-based-isohexane
#19
Jun Jin, Liyou Zheng, Warda Mwinyi Pembe, Jinfang Zhang, Dan Xie, Xiaosan Wang, Jianhua Huang, Qingzhe Jin, Xingguo Wang
High-purity isohexane containing 88.12% 2-methylpentane, which has a higher polarity than industrial hexane, was selected to selectively fractionate mango kernel fat to produce 1,3-distearoyl-2-oleoyl-glycerol (SOS)-rich fat. The three-stage fractionation process was optimized by considering the stearin yield and its SOS content to obtain a third stearin (TS). This fat contained a high percentage of SOS (69.2%) with only 0.8% diacylglycerol. Mass spectra and sn-2 fatty acid analyses further revealed that the TS was mainly composed of symmetrical monounsaturated triacylglycerols...
November 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28489022/lactic-fermentation-as-an-efficient-tool-to-enhance-the-antioxidant-activity-of-tropical-fruit-juices-and-teas
#20
Amandine Fessard, Ashish Kapoor, Jessica Patche, Sophie Assemat, Mathilde Hoarau, Emmanuel Bourdon, Theeshan Bahorun, Fabienne Remize
Tropical fruits like pineapple, papaya, mango, and beverages such as green or black teas, represent an underestimated source of antioxidants that could exert health-promoting properties. Most food processing technologies applied to fruit beverages or teas result in an impairment of inherent nutritional properties. Conversely, we hypothesise that lactic acid fermentation may constitute a promising route to maintain and even improve the nutritional qualities of processed fruits. Using specific growth media, lactic acid bacteria were selected from the fruit phyllosphere diversity and fruit juice, with the latter undergoing acidification kinetics analyses and characterised for exopolysaccharide production...
May 10, 2017: Microorganisms
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