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https://www.readbyqxmd.com/read/29241060/a-novel-electrochemical-cytosensor-for-selective-and-highly-sensitive-detection-of-cancer-cells-using-binding-induced-dual-catalytic-hairpin-assembly
#1
Ye Zhang, Shihua Luo, Bo Situ, Zhixin Chai, Bo Li, Jumei Liu, Lei Zheng
Rare cancer cells in body fluid could be useful biomarkers for noninvasive diagnosis of cancer. However, detection of these rare cells is currently challenging. In this work, a binding-induced dual catalytic hairpin assembly (DCHA) electrochemical cytosensor was developed for highly selective and sensitive detection of cancer cells. The fuel probe, released by hybridization between the capture probe and catalytic hairpin assembly (CHA) products of target cell-responsive reaction, initiated dual CHA recycling, leading to multiple CHA products...
December 7, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29240719/pericardial-effusion-as-a-presenting-symptom-of-hashimoto-thyroiditis-a-case-report
#2
Alberto Leonardi, Laura Penta, Marta Cofini, Lucia Lanciotti, Nicola Principi, Susanna Esposito
Background: Hashimoto thyroiditis (HT) is the most frequent cause of acquired hypothyroidism in paediatrics. HT is usually diagnosed in older children and adolescents, mainly in females and is rare in infants and toddlers with cardiac involvement, including pericardial effusion, that can be found in 10% to 30% of adult HT cases. In this paper, a child with HT and pericardial effusion as the most important sign of HT is described. Case presentation: A four-year-old male child suffering for a few months from recurrent abdominal pain sometimes associated with vomiting underwent an abdominal ultrasound scan outside the hospital...
December 14, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29239240/applications-of-mid-infrared-spectroscopy-in-the-clinical-laboratory-setting
#3
Sander De Bruyne, Marijn M Speeckaert, Joris R Delanghe
Fourier transform mid-infrared (MIR-FTIR) spectroscopy is a nondestructive, label-free, highly sensitive and specific technique that provides complete information on the chemical composition of biological samples. The technique both can offer fundamental structural information and serve as a quantitative analysis tool. Therefore, it has many potential applications in different fields of clinical laboratory science. Although considerable technological progress has been made to promote biomedical applications of this powerful analytical technique, most clinical laboratory analyses are based on spectroscopic measurements in the visible or ultraviolet (UV) spectrum and the potential role of FTIR spectroscopy still remains unexplored...
December 14, 2017: Critical Reviews in Clinical Laboratory Sciences
https://www.readbyqxmd.com/read/29239057/in-which-preterm-labor-patients-is-intravenous-maintenance-tocolysis-effective
#4
Satoshi Yoneda, Noriko Yoneda, Kaori Fukuta, Tomoko Shima, Akitoshi Nakashima, Arihiro Shiozaki, Osamu Yoshino, Mika Kigawa, Taketoshi Yoshida, Shigeru Saito
AIM: We evaluated whether maintenance tocolysis (intravenous ritodrine hydrochloride and/or magnesium sulfate) was effective in cases of spontaneous preterm labor with intact membranes. METHODS: One hundred and thirty preterm labor patients who reached 36 weeks of gestation by maintenance tocolysis were selected. Immediate delivery (ID) after ceasing maintenance tocolysis was defined as an 'effective case'. The correlated factors between ID and no immediate delivery (NID) were statistically analyzed...
December 14, 2017: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/29238038/fluid-consumption-pattern-and-hydration-among-8-14-years-old-children
#5
Dimitris Bougatsas, Giannis Arnaoutis, Demosthenes B Panagiotakos, Adam D Seal, Evan C Johnson, Jeanne H Bottin, Spiridoula Tsipouridi, Stavros A Kavouras
BACKGROUND/OBJECTIVES: Children consume various fluids to meet dietary water intake needs. However, the contribution of different fluid types on hydration is unclear. The purpose of this study was to develop fluid intake patterns and examine their association with hydration, as indicated by 24-h urine osmolality. SUBJECTS/METHODS: Two hundred ten (105 girls) healthy children (height: 1.49 ± 0.13 m, weight: 43.4 ± 12.6 kg, body fat: 25.2 ± 7.8%) recorded their fluid intake for two consecutive days, and collected their urine for 24-h during the 2nd day, while conducting their normal daily activities...
December 14, 2017: European Journal of Clinical Nutrition
https://www.readbyqxmd.com/read/29236055/hyaluronan-production-by-renomedullary-interstitial-cells-influence-of-endothelin-angiotensin-ii-and-vasopressin
#6
Sara Stridh, Fredrik Palm, Tomoko Takahashi, Mayumi Ikegami-Kawai, Malou Friederich-Persson, Peter Hansell
The content of hyaluronan (HA) in the interstitium of the renal medulla changes in relation to body hydration status. We investigated if hormones of central importance for body fluid homeostasis affect HA production by renomedullary interstitial cells in culture (RMICs). Simultaneous treatment with vasopressin and angiotensin II (Ang II) reduced HA by 69%. No change occurred in the mRNA expressions of hyaluronan synthase 2 (HAS2) or hyaluronidases (Hyals), while Hyal activity in the supernatant increased by 67% and CD44 expression reduced by 42%...
December 13, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29235389/extracellular-genomic-biomarkers-of-osteoarthritis
#7
Emma Budd, Giovanna Nalesso, Ali Mobasheri
Introduction Osteoarthritis (OA), a chronic, debilitating and degenerative disease of the joints, is the most common form of arthritis. The seriousness of this prevalent and chronic disease is often overlooked. Disease modifying OA drug development is hindered by the lack of soluble biomarkers to detect OA early. The objective of OA biomarker research is to identify early OA prior to the appearance of radiographic signs and the development of pain. Areas covered This review has focused on extracellular genomic material that could serve as biomarkers of OA...
December 13, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29235031/the-persistence-and-photostabilizing-characteristics-of-benzotriazole-and-5-methyl-1h-benzotriazole-reduce-the-photochemical-behavior-of-common-photosensitizers-and-organic-compounds-in-aqueous-environments
#8
Kenneth Hsien-Yung Chung, Yen-Ching Lin, Angela Yu-Chen Lin
Benzotriazole (BT) and 5-methyl-1H-benzotriazole (5-MeBT) are broadly used in industrial applications, such as anti-icing fluids and dishwashing detergent, and act as the primary building blocks for UV absorbers and photostabilizers. This study examined the occurrence of these two compounds in the environment and their unique photochemical behavior affecting photosensitizers and other micro-organic pollutants in aqueous environments. BT and 5-MeBT were detected in all river water samples from the major rivers in Taipei City in the concentration ranges of 147 to 1560 ng/L and 22 to 235 ng/L, respectively, and both compounds persisted through a conventional wastewater treatment plant...
December 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29234666/technical-advances-to-study-extracellular-vesicles
#9
REVIEW
Paula Carpintero-Fernández, Juan Fafián-Labora, Ana O'Loghlen
Extracellular vesicles are a heterogeneous and dynamic group of lipid bilayer membrane nanoparticles that can be classified into three different groups depending on their cellular origin: exosomes, microvesicles, and apoptotic bodies. They are produced by different cell types and can be isolated from almost all body fluids. EVs contain a variety of proteins, lipids, nucleic acids, and metabolites which regulate a number of biological and pathological scenarios both locally and systemically. Different techniques have been described in order to determine EV isolation, release, uptake, and cargo...
2017: Frontiers in Molecular Biosciences
https://www.readbyqxmd.com/read/29234150/the-shallow-structure-of-solfatara-volcano-italy-revealed-by-dense-wide-aperture-seismic-profiling
#10
Pier Paolo G Bruno, Stefano Maraio, Gaetano Festa
Two active-source, high-resolution seismic profiles were acquired in the Solfatara tuff cone in May and November 2014, with dense, wide-aperture arrays. Common Receiver Surface processing was crucial in improving signal-to-noise ratio and reflector continuity. These surveys provide, for the first time, high-resolution seismic images of the Solfatara crater, depicting a ~400 m deep asymmetrical crater filled by volcanoclastic sediments and rocks and carved within an overall non-reflective pre-eruptive basement showing features consistent with the emplacement of shallow intrusive bodies...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233934/chronic-infusion-of-taurolithocholate-into-the-brain-increases-fat-oxidation-in-mice
#11
Hannah M Eggink, Lauren L Tambyrajah, Rosa van den Berg, Isabel Mol, Jose K van den Heuvel, Martijn Koehorst, Albert K Groen, Anita Boelen, Andries Kalsbeek, Johannes A Romijn, Patrick C N Rensen, Sander Kooijman, Maarten R Soeters
Bile acids can function in the postprandial state as circulating signaling molecules in the regulation of glucose and lipid metabolism via the transmembrane receptor TGR5 and nuclear receptor FXR. Both receptors are present in the central nervous system, but their function in the brain is unclear. Therefore, we investigated the effects of intracerebroventricular (icv) administration of taurolithocholate (tLCA), a strong TGR5 agonist, and GW4064, a synthetic FXR agonist, on energy metabolism. We determined the effects of chronic icv infusion of tLCA, GW4064, or vehicle on energy expenditure, body weight and composition as well as tissue specific fatty acid uptake in mice equipped with osmotic minipumps...
December 12, 2017: Journal of Endocrinology
https://www.readbyqxmd.com/read/29233618/nutritional-impact-on-gene-expression-and-competence-of-oocytes-used-to-support-embryo-development-and-livebirth-by-cloning-procedures-in-goats
#12
C C L Fernandes, L H Aguiar, C E M Calderón, A M Silva, J P M Alves, R Rossetto, L R Bertolini, M Bertolini, D Rondina
Changes in the nutritional plan have been shown to affect oocyte quality, crucial to oocyte donors animals used in cloning. This study aimed to evaluate the impact of diets with increasing nutritional levels (maintenance diet=M; 1.3M; 1.6M; 1.9M) fed to goats for four weeks on follicular fluid composition, gene expression and oocyte competence used to cloning in goats. Donor females were superovulated for the retrieval of matured oocytes and physical measurements reported. After four weeks, groups receiving diets above maintenance increased thickness of subcutaneous adipose tissue and body weight, with higher values in 1...
October 19, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29232945/ultraviolet-rays-induced-sea-cucumber-stichopus-japonicus-melting-is-mediated-through-the-caspase-dependent-mitochondrial-apoptotic-pathway
#13
Li Su, Jing-Feng Yang, Xi Fu, Liang Dong, Da-Yong Zhou, Li-Ming Sun, Zhenwei Gong
Sea cucumber body wall melting occurs under certain circumstances. We have shown that apoptosis, but not autolysis, plays a critical role in the initial stage. However, it is still unclear how apoptosis is triggered in this process. In this study, we examined the reactive oxygen species (ROS), levels of B-cell lymphoma-2 (Bcl-2) and Bcl-2 Associated X Protein (Bax) and the depolarization of mitochondria transmembrane potential and Cytochrome c (Cyt c) release during the sea cucumber melting induced by ultraviolet (UV) exposure...
December 12, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29232914/characterization-and-bone-response-of-carbonate-containing-apatite-coated-titanium-implants-using-an-aqueous-spray-coating
#14
Ryo Yagi, Chihiro Mochizuki, Mitsunobu Sato, Takeshi Toyama, Masatsugu Hirota, Tohru Hayakawa, Chikahiro Ohkubo
We performed thin carbonate-containing apatite (CA) coating on titanium (Ti) by an aqueous spray coating (ASC) method that consisted of a Ca-CO₃-PO₄ complex. Two different CA coatings were produced by two different spray amounts and were heat-treated after spraying. We evaluated three-dimensional structures, adhesiveness to Ti, and durability of the CA film. In addition, we performed immersion experiments in simulated body fluid (SBF), and bone responses were evaluated after implantation into a femoral bone defect in rats...
December 11, 2017: Materials
https://www.readbyqxmd.com/read/29232054/high-speed-microscopy-for-in-vivo-monitoring-of-lymph-dynamics
#15
Mustafa Sarimollaoglu, Amanda J Stolarz, Dmitry A Nedosekin, Brittney R Garner, Terry W Fletcher, Ekaterina I Galanzha, Nancy J Rusch, Vladimir P Zharov
The lymphatic system contributes to body homeostasis by clearing fluid, lipids, plasma proteins, and immune cells from the interstitial space. Many studies have been performed to understand lymphatic function under normal conditions and during disease. Nevertheless, a further improvement in quantification of lymphatic behavior is needed. Here, we present advanced bright-field microscopy for in vivo imaging of lymph vessels and automated quantification of lymphatic function at temporal resolution of 2 ms. Full frame videos were compressed and recorded continuously at up to 540 frames per second...
December 12, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29231967/therapeutic-potential-of-porcine-bronchoalveolar-fluid-derived-mesenchymal-stromal-cells-in-a-pig-model-of-lps-induced-ali
#16
Mahesh Khatri, Levi Arthur Richardson
In this study, we isolated mesenchymal stromal (stem) cells (MSCs) from broncho-alveolar lavage fluid (BAL) of 2-6 week-old commercial pigs. BAL-MSCs displayed fibroblastic morphology and possessed self-renewal properties. Similar to bone-marrow MSCs, BAL-MSCs expressed mesenchymal markers and both cell types lacked the expression of hematopoetic markers. BAL-MSCs, when cultured in differentiation induction media, differentiated into adipocytes, osteocytes and chondrocytes. Next, we examined if BAL-MSCs have the ability to treat lipopolysaccharide (LPS)-induced acute lung injury (ALI) in a pig model...
December 12, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29231956/gender-diversity-sexual-orientation-and-bone-health-in-the-maturing-dancer
#17
REVIEW
Derrick D Brown
Gender identity forms an important aspect of still developing youths. Gender is traditionally classified into two distinct categories, female and male. Transgender is a general term that describes individuals who self-identity as male, female, both, or neither (i.e., gender fluid) and do not match the assigned at-birth gender. Adolescent transgender individuals are currently a marginal population. There are limited demographics on youths, but as of 2014 an estimate of 1.4 million adults in the USA identified as transgender...
December 2017: Medical Problems of Performing Artists
https://www.readbyqxmd.com/read/29231911/-difficulties-in-classifying-body-cavity-fluids-into-transudate-exudate-depending-on-the-various-diagnostic-criteria
#18
Olga M Koper, Joanna Kamińska, Katarzyna Pańkowska, Anna Matosek, Edyta Suchodoła, Karolina Sprawka, Anna Tenderenda, Paulina Brania, Agnieszka Statkiewicz, Halina Kemona
Disease processes may impair the production and reabsorption of fluid from in the body cavities, which results in its excessive accumulation. AIM: The aim of the study was the evaluation of difficulties regarding the classification of fluids from the body cavities into transudate/exudate observing the following: Light's criteria, total fluid protein concentration, and total protein ratio (TP ratio) and lactate dehydrogenase ratio (LDH ratio). MATERIALS AND METHODS: Retrospective analysis was conducted on pleural (N=314), peritoneal (N=114) and pericardial (N=10) fluids, which were tested for the total protein concentration and LDH activity both in fluid and serum and calculated on TP ratio and LDH ratio...
November 23, 2017: Polski Merkuriusz Lekarski: Organ Polskiego Towarzystwa Lekarskiego
https://www.readbyqxmd.com/read/29231896/differential-expression-of-serum-micrornas-supports-cd4%C3%A2-%C2%BA-t-cell-differentiation-into-th2-th17-cells-in-severe-equine-asthma
#19
Alicja Pacholewska, Matthias F Kraft, Vincent Gerber, Vidhya Jagannathan
MicroRNAs (miRNAs) regulate post-transcriptional gene expression and may be exported from cells via exosomes or in partnership with RNA-binding proteins. MiRNAs in body fluids can act in a hormone-like manner and play important roles in disease initiation and progression. Hence, miRNAs are promising candidates as biomarkers. To identify serum miRNA biomarkers in the equine model of asthma we investigated small RNA derived from the serum of 34 control and 37 asthmatic horses. These samples were used for next generation sequencing, novel miRNA identification and differential miRNA expression analysis...
December 12, 2017: Genes
https://www.readbyqxmd.com/read/29231737/stability-of-polyethylene-glycol-and-zwitterionic-surface-modifications-in-pdms-microfluidic-flow-chambers
#20
Thomas James Plegue, Kyle M Kovach, Alex J Thompson, Joseph A Potkay
Blood-material interactions are crucial to the lifetime, safety, and overall success of a blood contacting devices. Hydrophilic polymer coatings have been employed to improve device lifetime by shielding blood contacting materials from the natural foreign body response, primarily the intrinsic pathway of the coagulation cascade. These coatings have the ability to repel proteins, cells, bacteria, and other micro-organisms. Coatings are desired to have long-term stability, so that the nonthrombogenic and nonfouling effects gained are long lasting...
December 12, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
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