keyword
MENU ▼
Read by QxMD icon Read
search

Microbiology automation

keyword
https://www.readbyqxmd.com/read/29153182/a-100-year-review-cheese-production-and-quality
#1
M E Johnson
In the beginning, cheese making in the United States was all art, but embracing science and technology was necessary to make progress in producing a higher quality cheese. Traditional cheese making could not keep up with the demand for cheese, and the development of the factory system was necessary. Cheese quality suffered because of poor-quality milk, but 3 major innovations changed that: refrigeration, commercial starters, and the use of pasteurized milk for cheese making. Although by all accounts cold storage improved cheese quality, it was the improvement of milk quality, pasteurization of milk, and the use of reliable cultures for fermentation that had the biggest effect...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29140977/low-cost-and-open-source-multi-fluorescence-imaging-system-for-teaching-and-research-in-biology-and-bioengineering
#2
Isaac Nuñez, Tamara Matute, Roberto Herrera, Juan Keymer, Timothy Marzullo, Timothy Rudge, Fernán Federici
The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates...
2017: PloS One
https://www.readbyqxmd.com/read/29135992/internal-quality-assurance-in-diagnostic-microbiology-a-simple-approach-for-insightful-data
#3
Valentin Scherz, Christian Durussel, Gilbert Greub
Given the importance of microbiology results on patient care, high quality standards are expected. Internal quality assurance (IQA) could mitigate the limitations of internal quality control, competency assessment and external quality assurance, adding a longitudinal insight, including pre- and post-analytical steps. Here, we implemented an IQA program in our clinical microbiology facilities with blind resubmission of routine samples during 22 months. One-hundred-and-twenty-one out of 123 (98.4%) serological analyses and 112 out of 122 (91...
2017: PloS One
https://www.readbyqxmd.com/read/29118410/the-microbiological-characteristics-and-risk-factors-for-picc-related-bloodstream-infections-in-intensive-care-unit
#4
Shumin Zhang, Xiaofeng Sun, Yan Lei
The study was aimed to investigate the pathogens distribution and risk factors for PICC-related bloodstream infection in intensive care unit (ICU) patients. 402 patients placed with PICC in ICU were recruited in the study. The microbiological characteristics of PICC-related infection were investigated by Vitek 2 Compact automated microbial system. Antibiotics sensitivity was performed with disk diffusion and minimum inhibitory concentration (MIC) methods. Multivariate logistic and cox analyses were performed to identify the risk factors for PICC-related infection in ICU patients...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29118171/total-laboratory-automation-and-maldi-tof-improve-turnaround-times-in-the-clinical-microbiology-laboratory-a-retrospective-analysis
#5
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker MALDI-TOF and BD Kiestra Total Laboratory Automation (TLA) three and four years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TAT) for positive and negative urine cultures before and after implementation. Comparison I: TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF for organism identification...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29118168/use-of-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-id-ast-of-pathogens-in-positive-blood-cultures-and-impact-on-time-to-results-and-workflow
#6
Angella Charnot-Katsikas, Vera Tesic, Nedra Love, Brandy Hill, Cindy Bethel, Sue Boonlayangoor, Kathleen G Beavis
The Accelerate Pheno™ system uses automated fluorescence in situ hybridization technology with morphokinetic cellular analysis to provide rapid species identification (ID) and antimicrobial susceptibility testing (AST) results for the most commonly identified organisms in bloodstream infections.Objective was to evaluate the accuracy and workflow of bacterial and yeast ID, and bacterial AST using the Accelerate Pheno™ system in the clinical microbiology laboratory.Consecutive fresh blood cultures received in the laboratory were analyzed by the Accelerate Pheno™ system within 0 - 8 h of growth detection...
November 8, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29112122/identification-and-antimicrobial-susceptibility-testing-of-anaerobic-bacteria-rubik-s-cube-of-clinical-microbiology
#7
REVIEW
Márió Gajdács, Gabriella Spengler, Edit Urbán
Anaerobic bacteria have pivotal roles in the microbiota of humans and they are significant infectious agents involved in many pathological processes, both in immunocompetent and immunocompromised individuals. Their isolation, cultivation and correct identification differs significantly from the workup of aerobic species, although the use of new technologies (e.g., matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, whole genome sequencing) changed anaerobic diagnostics dramatically...
November 7, 2017: Antibiotics
https://www.readbyqxmd.com/read/29102604/fluorometric-determination-of-d-lactate-in-biological-fluids
#8
Torben Larsen
OBJECTIVE: D-lactic acid in the mammalian body is mainly of microbiological origin and is often located somewhere along the digestive tract. Surgical, extensive re-sectioning of the small bowel may be one of the risk factors for altered balance in the microbiological environment. Higher levels in the body may lead to D-lactate acidosis and neurotoxicity; consequently, the possibility of diagnosis of this condition is important. Several analytical procedures for D-lactate have been introduced, but it is absolutely mandatory to distinguish this metabolite from the much more abundant and naturally occurring stereoisomer L-lactate...
November 3, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29090008/automated-flow-cytometry-an-alternative-to-urine-culture-in-a-routine-clinical-microbiology-laboratory
#9
REVIEW
Patricia Mejuto, Mariam Luengo, Julio Díaz-Gigante
The urine culture is the "gold standard" for the diagnosis of urinary tract infections (UTI) but constitutes a significant workload in the routine clinical laboratory. Due to the high percentage of negative results, there is a need for an efficient screening method, with a high negative predictive value (NPV) that could reduce the number of unnecessary culture tests. With the purpose of improving the efficiency of laboratory work, several methods for screening out the culture-negative samples have been developed, but none of them has shown adequate sensitivity (SE) and high NPV...
2017: International Journal of Microbiology
https://www.readbyqxmd.com/read/29062487/challenges-in-the-identification-of-chryseobacterium-indologenes-and-elizabethkingia-meningoseptica-in-cases-of-nosocomial-infections-and-patients-with-cystic-fibrosis
#10
É B de Carvalho Filho, F A L Marson, C E Levy
Rare nonfermenting Gram-negative bacilli, such as Chryseobacterium indologenes and Elizabethkingia meningoseptica, have clinical importance in nosocomial infections and cystic fibrosis (CF), and their identification is a challenge to microbiology laboratories. Thus, the objective of this study was to verify the performance of phenotypic and mass spectrometry (matrix-assisted desorption ionization-time of flight mass spectrometry, MALDI-TOF MS) methods to identify C. indologenes and E. meningoseptica. In this context, the results obtained with phenotypic methods-namely manual biochemical and automated VITEK 2 (bioMérieux, Marcy l'Etoile, France) and Phoenix tests (Becton Dickinson (BD), San Diego, CA, USA)-and by MALDI-TOF MS-namely MALDI-TOF VITEK MS (MALDI-MS; bioMérieux) and MALDI-TOF BioTyper (MALDI-BD; BD)-of 22 isolates (blood cultures of patients with nosocomial infection (n = 15) and from patients with CF (n = 7)), initially identified as C...
November 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/29062009/comparison-of-four-automated-microbiology-systems-with-16s-rrna-gene-sequencing-for-identification-of-chryseobacterium-and-elizabethkingia-species
#11
Jiun-Nong Lin, Chung-Hsu Lai, Chih-Hui Yang, Yi-Han Huang, Hsiu-Fang Lin, Hsi-Hsun Lin
Chryseobacterium and Elizabethkingia species have recently emerged as causative agents in life-threatening infections in humans. We aimed to evaluate the rates at which four common microbial identification systems identify Chryseobacterium and Elizabethkingia species in clinical microbiology laboratories. Based on the results of 16S rRNA gene sequencing, a total of 114 consecutive bacteremic isolates, including 36 (31.6%) C. indologenes, 35 (30.7%) E. anophelis, 22 (19.3%) C. gleum, 13 (11.4%) E. meningoseptica, and other species, were included in this study...
October 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29046409/using-treponemal-assay-signal-strength-cut-off-ratios-to-predict-syphilis-infection
#12
Claire C Bristow, Jeffrey D Klausner
Syphilis screening using the reverse algorithm, a treponemal test for screening followed by a non-treponemal test if reactive, is increasingly being used. This algorithm has several advantages including use of an automated screening test, saving on laboratory time and costs as well as detection of very early syphilis infection. However, under this algorithm, in situations where the treponemal result is positive and the non-treponemal result is non-reactive a second treponemal test must be performed, which may actually lead to inefficiencies in the laboratory...
October 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29046166/evaluation-of-mericon-e-coli-o157-screen-plus-and-mericon-e-coli-stec-o-type-pathogen-detection-assays-in-select-foods-collaborative-study-first-action-2017-05
#13
Patrick Bird, M Joseph Benzinger, Benjamin Bastin, Erin Crowley, James Agin, David Goins, Marcia Armstrong
QIAGEN mericon Escherichia coli O157 Screen Plus and mericon E. coliShiga toxin-producing E. coli (STEC) O-Type Pathogen Detection Assays use Real-Time PCR technology for the rapid, accurate detection of E. coli O157 and the “big six” (O26, O45, O103, O111, O121, O145) (non-O157 STEC) in select food types. Using a paired study design, the assays were compared with the U.S. Department of Agriculture, Food Safety Inspection Service Microbiology Laboratory Guidebook Chapter 5.09 reference method for the detection of E...
October 18, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/29036747/prevention-of-multidrug-resistant-infections-from-contaminated-duodenoscopes-position-statement-of-the-european-society-of-gastrointestinal-endoscopy-esge-and-european-society-of-gastroenterology-nurses-and-associates-esgena
#14
Ulrike Beilenhoff, Holger Biering, Reinhard Blum, Jadranka Brljak, Monica Cimbro, Jean-Marc Dumonceau, Cesare Hassan, Michael Jung, Christiane Neumann, Michael Pietsch, Lionel Pineau, Thierry Ponchon, Stanislav Rejchrt, Jean-François Rey, Verona Schmidt, Jayne Tillett, Jeanin van Hooft
Patients should be informed about the benefits and risks of endoscopic retrograde cholangiopancreatography (ERCP)Only specially trained and competent personnel should carry out endoscope reprocessing.Manufacturers of duodenoscopes should provide detailed instructions on how to use and reprocess their equipment.In the case of modifications to their equipment, manufacturers should provide updated instructions for use.Detailed reprocessing protocols based on the manufacturer's instructions for use should clearly lay out the different reprocessing steps necessary for each endoscope model...
November 2017: Endoscopy
https://www.readbyqxmd.com/read/29035494/medical-devices-immunology-and-microbiology-devices-classification-of-the-automated-image-assessment-system-for-microbial-colonies-on-solid-culture-media-final-order
#15
(no author information available yet)
The Food and Drug Administration (FDA or we) is classifying the automated image assessment system for microbial colonies on solid culture media into class II (special controls). The special controls that apply to the device type are identified in this order and will be part of the codified language for the automated image assessment system for microbial colonies on solid culture media's classification. We are taking this action because we have determined that classifying the device into class II (special controls) will provide a reasonable assurance of safety and effectiveness of the device...
October 16, 2017: Federal Register
https://www.readbyqxmd.com/read/28983865/performance-of-automated-multiplex-pcr-using-sonication-fluid-for-diagnosis-of-periprosthetic-joint-infection-a-prospective-cohort
#16
Nora Renz, Susanne Feihl, Sabrina Cabric, Andrej Trampuz
PURPOSE: Sonication of explanted prostheses improved the microbiological diagnosis of periprosthetic joint infections (PJI). We evaluated the performance of automated multiplex polymerase chain reaction (PCR) using sonication fluid for the microbiological diagnosis of PJI. METHODS: In a prospective cohort using uniform definition criteria for PJI, explanted joint prostheses were investigated by sonication and the resulting sonication fluid was analyzed by culture and multiplex PCR...
December 2017: Infection
https://www.readbyqxmd.com/read/28961857/comparative-evaluation-of-vitek%C3%A2-2-and-three-commercial-gradient-strip-assays-for-daptomycin-susceptibility-testing-of-staphylococcus-aureus
#17
Robert E Weber, Franziska Layer, Ingo Klare, Guido Werner, Birgit Strommenger
Objectives: MRSA remains a major cause of severe nosocomial infections and the increased use of vancomycin and daptomycin for MRSA treatment over the last decade has led to the isolation of MRSA strains with decreased daptomycin susceptibility. In addition, a growing number of MSSA isolates with reduced susceptibility to daptomycin have been described lately. Surveillance of the emergence of such a daptomycin-non-susceptible MSSA population requires prompt and reliable daptomycin susceptibility testing...
November 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28954899/optimizing-test-utilization-in-the-clinical-microbiology-laboratory-tools-and-opportunities
#18
Bobbi S Pritt
Optimal laboratory test utilization is important for providing high quality clinical care and efficiently using limited health care resources. While microbiologists have long been advocates for appropriate laboratory test utilization, the wide-spread availability of electronic medical records capable of supporting clinician order entry and clinical decision support tools (CDSTs) have provided expanded opportunities for implementing effective, automated test stewardship protocols. In this issue of the Journal of Clinical Microbiology, D...
September 27, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28951981/prospective-evaluation-of-a-high-multiplexing-real-time-polymerase-chain-reaction-array-for-the-rapid-identification-and-characterization-of-bacteria-causative-of-nosocomial-pneumonia-from-clinical-specimens-a-proof-of-concept-study
#19
S Roisin, T-D Huang, R de Mendonça, C Nonhoff, P Bogaerts, M Hites, B Delaere, S Hamels, F de Longueville, Y Glupczynski, O Denis
The purpose of this study was evaluation of the VAPChip assay based on the "Rapid-Array-PCR-technology" which targets 13 respiratory pathogens and 24 β-lactam resistance genes directly on respiratory clinical specimens. The first step included analysis of 45 respiratory specimens in order to calibrate and determine the threshold for target genes. The second prospective step involved 85 respiratory samples from patients suspected of nosocomial pneumonia collected in two academic hospitals over an 8-month period...
September 27, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28950760/xpert-flu-as-a-rapid-diagnostic-test-for-respiratory-tract-viral-infection-evaluation-and-implementation-as-a-24-7-service
#20
Sofie Haglund, Maysae Quttineh, Annette Nilsson Bowers, Andreas Matussek, Anna J Henningsson
A rapid and reliable influenza method is important to meet diagnostic needs. Here, the performance of the cartridge-based Xpert Flu assay was evaluated in comparison with an in-house real-time PCR method. Analysis was then decentralized from the clinical microbiology laboratory located at County Hospital Ryhov, Jönköping, Sweden, to the clinical chemistry laboratories present at the three hospitals in Region Jönköping County, allowing a 24/7 service. In total, 189 clinical samples were included in 2014-2015...
September 26, 2017: Infectious Diseases
keyword
keyword
66331
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"