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https://www.readbyqxmd.com/read/28916321/analysis-of-resistance-genes-in-pan-resistant-myroides-odoratimimus-clinical-strain-pr63039-using-whole-genome-sequencing
#1
Desong Ming, Qing-Qing Chen, Xiao-Tin Chen
To clarify the antibiotic resistance mechanisms of Myroides odoratimimus, pan-resistant M. odoratimimus strain PR63039 was isolated and its genome sequenced and analyzed. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method, and the Phoenix-100 Automated Microbiology System with a NMIC/ID-4 panel including aminoglycosides, β-lactams, polypeptides, quinolones, sulfonamides, chloramphenicols, and tetracyclines. Single-molecule real-time whole genome sequencing was conducted using the PacBio RSII system, and genome annotation was performed using RAST and IMG ER...
September 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28900357/five-year-microbiological-monitoring-of-wards-and-operating-theatres-in-southern-italy
#2
REVIEW
V La Fauci, C Genovese, A Facciolà, M A R Palamara, R Squeri
INTRODUCTION: Nosocomial infections are one of the greatest problems in public health. Several studies have highlighted the role played by the hospital environment as a possible source of transmission of nosocomial pathogens. METHODS: A five-year monitoring of bacterial contamination on healthcare workers hands, surfaces most closely in contact with inpatient wards, operating theatres and "at rest" and "in use" operating theatre air samples. For the samples, we used sterile swabs, contact slides, manual API, and automated VITEK systems for identification...
June 2017: Journal of Preventive Medicine and Hygiene
https://www.readbyqxmd.com/read/28892765/the-performance-of-luminex-aries-%C3%A2-flu-a-b-rsv-and-cepheid-xpert-%C3%A2-flu-rsv-xc-for-the-detection-of-influenza-a-influenza-b-and-respiratory-syncytial-virus-in-prospective-patient-samples
#3
Phillip McMullen, Sue Boonlayangoor, Angella Charnot-Katsikas, Kathleen G Beavis, Vera Tesic
BACKGROUND: The demand for rapid, accurate viral testing has increased the number of assays available for the detection of viral pathogens. One of the newest FDA cleared platforms is the Luminex ARIES(®) Flu A/B & RSV, which is a fully automated, real-time PCR-based assay used for detection of influenza A, influenza B, and respiratory syncytial virus (RSV). OBJECTIVES: We sought to compare the performance of Luminex ARIES(®) Flu A/B & RSV assay to the Cepheid Xpert(®) Flu/RSV XC assay for rapid Flu and RSV testing...
September 5, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28885919/diagnostics-in-a-forward-deployed-setting
#4
Brett Swierczewski, Mark Simons
Current diagnostic methods for enteric pathogens include conventional/traditional microbiology, microscopy, enzyme immunoassay, automated identification platforms, and molecular methods. The choice of diagnostic test in the forward deployed military setting often depends on turnaround time, potential etiologic agents, costs, and laboratory capabilities. The military operational environment presents many challenges that impact the practicality and performance of even robust diagnostic platforms. With recent developments in diagnostic platforms and availability of high-performance multiplex molecular methods to pair with traditional culture and antibiotic susceptibility tests, there are more opportunities to gather information on the etiologic causes and clinical impacts of traveler's diarrhea, both in civilians and in deployed military populations...
September 2017: Military Medicine
https://www.readbyqxmd.com/read/28884631/takotsubo-cardiomyopathy-associated-with-paragonimiasis-westermani
#5
Ryouhei Matsuoka, Jun Muneuchi, Yusaku Nagatomo, Daisuke Shimizu, Seigo Okada, Chiaki Iida, Hiromitsu Shirouzu, Mamie Watanabe, Yasuhiko Takahashi, Haruhiko Maruyama
An 11-year-old boy collapsed during morning assembly at his junior high school. The automated external defibrillator detected ventricular fibrillation and provided shock delivery. He was successfully resuscitated and reverted to sinus rhythm. Electrocardiography showed ST-T elevation in the precordial leads. Echocardiography and angiography demonstrated akinesia of the apex and mid-wall of the left ventricle with preserved contraction of the basal segments, which suggested Takotsubo cardiomyopathy. The patient and his family had often eaten uncooked crab, and his father had a past history of infection with Paragonimiasis westermani...
September 8, 2017: Paediatrics and International Child Health
https://www.readbyqxmd.com/read/28869004/performance-assessment-of-urine-flow-cytometry-ufc-to-screen-urines-to-reflex-to-culture-in-immunocompetent-and-immunosuppressed-hosts
#6
Aleksandra Stefanovic, Diane Roscoe, Romali Ranasinghe, Titus Wong, Elizabeth Bryce, Charlene Porter, Adelina Lim, Jennifer Grant, Karen Ng, Morris Pudek
PURPOSE: Urine flow cytometry (UFC) is an automated method to quantify bacterial and white blood cell (WBC) counts. We aimed to determine whether a threshold for these parameters can be set to use UFC as a sensitive screen to predict which urine samples will subsequently grow in culture. METHODOLOGY: Urines submitted to our microbiology laboratory at a tertiary care centre from 22 July 2015-17 February 2016 underwent UFC (Sysmex UF-1000i) analysis, regular urinalysis and urine culture...
September 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28844384/implementation-and-evaluation-of-an-automated-surveillance-system-to-detect-hospital-outbreak
#7
Anna Stachel, Gabriela Pinto, John Stelling, Yi Fulmer, Bo Shopsin, Kenneth Inglima, Michael Phillips
BACKGROUND: The timely identification of a cluster is a critical requirement for infection prevention and control (IPC) departments because these events may represent transmission of pathogens within the health care setting. Given the issues with manual review of hospital infections, a surveillance system to detect clusters in health care settings must use automated data capture, validated statistical methods, and include all significant pathogens, antimicrobial susceptibility patterns, patient care locations, and health care teams...
August 22, 2017: American Journal of Infection Control
https://www.readbyqxmd.com/read/28840987/multicenter-evaluation-of-an-image-analysis-device-apas-comparison-between-digital-image-and-traditional-plate-reading-using-urine-cultures
#8
John Glasson, Rhys Hill, Michael Summerford, Dianne Olden, Fotula Papadopoulos, Stephen Young, Steven Giglio
BACKGROUND: The application of image analysis technologies for the interpretation of microbiological cultures is evolving rapidly. The primary aim of this study was to establish whether the image analysis system named Automated Plate Assessment System (APAS; LBT Innovations Ltd., Australia) could be applied to screen urine cultures. A secondary aim was to evaluate differences between traditional plate reading (TPR) and the reading of cultures from images, or digital plate reading (DPR)...
November 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28837780/development-of-mast-a-microscopy-based-antimicrobial-susceptibility-testing-platform
#9
Kenneth P Smith, David L Richmond, Thea Brennan-Krohn, Hunter L Elliott, James E Kirby
Antibiotic resistance is compromising our ability to treat bacterial infections. Clinical microbiology laboratories guide appropriate treatment through antimicrobial susceptibility testing (AST) of patient bacterial isolates. However, increasingly, pathogens are developing resistance to a broad range of antimicrobials, requiring AST of alternative agents for which no commercially available testing methods are available. Therefore, there exists a significant AST testing gap in which current methodologies cannot adequately address the need for rapid results in the face of unpredictable susceptibility profiles...
August 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28792758/profiling-of-microbial-colonies-for-high-throughput-engineering-of-multistep-enzymatic-reactions-via-optically-guided-matrix-assisted-laser-desorption-ionization-mass-spectrometry
#10
Tong Si, Bin Li, Troy J Comi, Yuwei Wu, Pingfan Hu, Yuying Wu, Yuhao Min, Douglas A Mitchell, Huimin Zhao, Jonathan V Sweedler
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-ToF) mass spectrometry (MS) imaging has been used for rapid phenotyping of enzymatic activities, but is mainly limited to single-step conversions. Herein we report a label-free method for high-throughput engineering of multistep biochemical reactions based on optically guided MALDI-ToF MS analysis of bacterial colonies. The bacterial cells provide containment of multiple enzymes and access to substrates and cofactors via metabolism. Automated MALDI-ToF MS acquisition from randomly distributed colonies simplifies procedures to prepare strain libraries without liquid handling...
September 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28700901/hyperspectral-image-analysis-for-rapid-and-accurate-discrimination-of-bacterial-infections-a-benchmark-study
#11
Simone Arrigoni, Giovanni Turra, Alberto Signoroni
With the rapid diffusion of Full Laboratory Automation systems, Clinical Microbiology is currently experiencing a new digital revolution. The ability to capture and process large amounts of visual data from microbiological specimen processing enables the definition of completely new objectives. These include the direct identification of pathogens growing on culturing plates, with expected improvements in rapid definition of the right treatment for patients affected by bacterial infections. In this framework, the synergies between light spectroscopy and image analysis, offered by hyperspectral imaging, are of prominent interest...
September 1, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28689506/description-and-validation-of-a-new-automated-surveillance-system-for-clostridium-difficile-in-denmark
#12
M Chaine, S Gubbels, M Voldstedlund, B Kristensen, J Nielsen, L P Andersen, S Ellermann-Eriksen, J Engberg, A Holm, B Olesen, H C Schønheyder, C Østergaard, S Ethelberg, K Mølbak
The surveillance of Clostridium difficile (CD) in Denmark consists of laboratory based data from Departments of Clinical Microbiology (DCMs) sent to the National Registry of Enteric Pathogens (NREP). We validated a new surveillance system for CD based on the Danish Microbiology Database (MiBa). MiBa automatically collects microbiological test results from all Danish DCMs. We built an algorithm to identify positive test results for CD recorded in MiBa. A CD case was defined as a person with a positive culture for CD or PCR detection of toxin A and/or B and/or binary toxin...
September 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28687186/evaluation-of-a-new-packaging-process-for-non-autoclavable-endoscopes-results-for-the-first%C3%A2-100%C3%A2-microbiological-samples
#13
C Minebois, P Saviuc, J Shum, P Tuvignon, I Pelloux, M-P Brenier-Pinchart, C Landelle, M-R Mallaret
BACKGROUND: A new process for packaging endoscopes (SureStore(®), Medical Innovations Group) immediately after they exit from washing and disinfection in an automated endoscope reprocessor (AER) allows for endoscopes to be stored for up to 15 days. AIM: To describe the microbiological quality of samples from gastrointestinal endoscopes following this process. METHODS: Three-month prospective study using microbiological sampling from a stock of 38 gastrointestinal endoscopes carried out in a French University Hospital...
July 4, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28680970/nbiochip-a-lab-on-a-chip-platform-of-mono-and-polymicrobial-biofilms-for-high-throughput-downstream-applications
#14
Anand Srinivasan, Nelson S Torres, Kai P Leung, Jose L Lopez-Ribot, Anand K Ramasubramanian
Current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (HT) applications. We have developed a novel fully automated platform for the formation of mono- and polymicrobial biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans at the nanoscale level. The nBioChip is robotically printed, robustly handled, and scanned using a standard microarray reader...
May 2017: MSphere
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-expanded-polytetrafluoroethylene-graft-sodding
#15
Stuart K Williams, Marvin E Morris, Paul E Kosnik, Kevin D Lye, Gary D Gentzkow, Charles B Ross, Amit J Dwevidi, Leigh B Kleinert
Adipose-derived stromal vascular fraction (SVF) cell populations are being evaluated for numerous clinical applications. The current study evaluated a point-of-care technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived SVF cells to be used in the fabrication of a tissue-engineered vascular graft in the operating room. A total of seven patients were enrolled in this study and received femoral to tibial expanded polytetrafluoroethylene bypass grafts to treat peripheral arterial disease...
August 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28657416/maldi-tof-mass-spectrometry-provides-high-accuracy-in-identification-of-salmonella-at-species-level-but-is-limited-to-type-or-subtype-salmonella-serovars
#16
Lin Kang, Nan Li, Ping Li, Yang Zhou, Shan Gao, Hongwei Gao, Wenwen Xin, Jinglin Wang
Salmonella can cause global foodborne illnesses in humans and many animals. The current diagnostic gold standard used for detecting Salmonella infection is microbiological culture followed by serological confirmation tests. However, these methods are complicated and time-consuming. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis offers some advantages in rapid identification, for example, simple and fast sample preparation, fast and automated measurement, and robust and reliable identification up to genus and species levels, possibly even to the strain level...
April 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/28651665/using-milk-leukocyte-differentials-for-diagnosis-of-subclinical-bovine-mastitis
#17
Juliano Leonel Gonçalves, Roberta L Lyman, Mitchell Hockett, Rudy Rodriguez, Marcos Veiga Dos Santos, Kevin L Anderson
This research study aimed to evaluate the use of the milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize the milk leukocyte responses to specific groups of pathogens causing subclinical mastitis. The MLD measures the absolute number and relative percentage of inflammatory cells in milk samples. Using the MLD in two dairy herds (170 and 172 lactating cows, respectively), we studied all lactating cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 × 103 cells/ml...
June 27, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28638468/current-and-emerging-techniques-for-antibiotic-susceptibility-tests
#18
REVIEW
Karan Syal, Manni Mo, Hui Yu, Rafael Iriya, Wenwen Jing, Sui Guodong, Shaopeng Wang, Thomas E Grys, Shelley E Haydel, Nongjian Tao
Infectious diseases caused by bacterial pathogens are a worldwide burden. Serious bacterial infection-related complications, such as sepsis, affect over a million people every year with mortality rates ranging from 30% to 50%. Crucial clinical microbiology laboratory responsibilities associated with patient management and treatment include isolating and identifying the causative bacterium and performing antibiotic susceptibility tests (ASTs), which are labor-intensive, complex, imprecise, and slow (taking days, depending on the growth rate of the pathogen)...
2017: Theranostics
https://www.readbyqxmd.com/read/28625155/applications-of-optical-dna-mapping-in-microbiology
#19
Diana Bogas, Lena Nyberg, Rui Pacheco, Nuno F Azevedo, Jason P Beech, Margarita Gomila, Jorge Lalucat, Célia M Manaia, Olga C Nunes, Jonas O Tegenfeldt, Fredrik Westerlund
Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches...
June 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28566082/-femoral-hemodialysis-catheter-related-bacteremia-due-to-globicatella-sanguinis-challenges-in-species-identification
#20
Elif Aktaş, Nafia Canan Gürsoy, Tamer Sakacı, Yener Koç, Aziz Ahmad Hamidi, Emin Bulut, Duygu Erdemir, Barış Otlu
In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2...
April 2017: Mikrobiyoloji Bülteni
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