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https://www.readbyqxmd.com/read/27837517/microarray-technologies-in-fungal-diagnostics
#1
Steffen Rupp
Microarray technologies have been a major research tool in the last decades. In addition they have been introduced into several fields of diagnostics including diagnostics of infectious diseases. Microarrays are highly parallelized assay systems that initially were developed for multiparametric nucleic acid detection. From there on they rapidly developed towards a tool for the detection of all kind of biological compounds (DNA, RNA, proteins, cells, nucleic acids, carbohydrates, etc.) or their modifications (methylation, phosphorylation, etc...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27805525/microbiologic-investigation-of-patients-before-living-donor-liver-transplant
#2
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Chronic infections can cause serious complications and create morbidity and mortality for affected patients during immunosuppressive therapy in the posttransplant period. Here, we studied the microbiologic screening results of patients before living-donor liver transplant. MATERIALS AND METHODS: The microbiologic screening results of 25 patients during 2015 at National Scientific Research Center (Astana, Kazakhstan) before livingdonor liver transplant were prospectively analyzed...
November 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27805513/microbiologic-monitoring-of-kidney-transplant-recipients-before-transplant
#3
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Drug immunosuppression, traditionally used to minimize autoimmune developments after liver, kidney, heart, and lung transplant, and which requires high doses of corticosteroids, creates favorable conditions for the development of various infectious processes and the existence of pathogens, which can be activated after transplant. This study aimed to investigate the bacterial pathogens in patients before living-donor kidney transplant. MATERIALS AND METHODS: We prospectively investigated 117 clinical samples of 33 patients who were seen during 2015 at the National Scientific Research Center (Astana, Kazakhstan) before living-donor kidney transplant...
November 2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27801488/peritoneal-dialysis-experience-of-the-department-of-pediatrics-of-the-hospital-charles-nicolle-of-tunis
#4
Manel Jellouli, Meriem Ferjani, Amal Oueslati, Kamel Abidi, Ouns Naija, Yousra Hammi, Taieb Ben Abdallah, Tahar Gargah
Introduction Peritoneal dialysis (PD) is still the most common modality used in treatment for children with End Stage Renal Disease (ESRD). The objective of this study was to identify the epidemiological, clinical, and microbiological factors affecting the outcome of PD. Methods In this study, we retrospectively reviewed the records of 85 patients who were treated with DP for the last ten years (from January 2004 to December 2013) in the Department of Pediatrics in Charles Nicolle hospital, Tunis. Results The mean duration of PD was 18...
May 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/27766214/an-overview-on-novel-microbial-determination-methods-in-pharmaceutical-and-food-quality-control
#5
Mahboob Nemati, Aliasghar Hamidi, Solmaz Maleki Dizaj, Vahid Javaherzadeh, Farzaneh Lotfipour
Traditional microbiological methods tend to be labor-intensive and time-consuming. Rapid and novel methods in microbiological tests provide more sensitive, precise and reproducible results compared with conventional methods. In microbiology, the most rapid testing methods belong to the field of biotechnology such as PCR, ELISA, ATP bioluminescence and etc. Nevertheless impedance microbiology, biosensors and analytical procedures to determine microbial constituents are of significance. The present review article was conducted using internet databases and related scientific literatures and articles that provide information on developments in the rapid methods in microbiology...
September 2016: Advanced Pharmaceutical Bulletin
https://www.readbyqxmd.com/read/27763479/an-appropriately-performed-conventional-blood-culture-can-facilitate-choice-of-therapy-in-resource-constrained-settings-comparison-with-bactec-9050
#6
P V Surase, G Nataraj, K Pattamadai, P R Mehta, A R Pazare, M C Agarwal, R N Nanavati
AIMS: Comparison of conventional blood culture with BACTEC 9050 for rate and time to detection of microorganisms. SETTINGS AND DESIGN: A prospective study was carried out in a multispecialty tertiary care teaching hospital. SUBJECTS AND METHODS: A total of 835 paired specimens (797 blood and 38 nonblood specimens) were collected and processed according to standard microbiological procedures by both conventional method as well as by BACTEC 9050 automated culture system...
October 2016: Journal of Postgraduate Medicine
https://www.readbyqxmd.com/read/27739025/prevalence-of-neisseria-meningitidis-carriage-with-identification-of-serogroups-and-genogroups-in-professional-soldiers
#7
Krzysztof Korzeniewski, M Konior, M Kiedrowska, E Wódka, E Zwolińska, A Skoczyńska
The article presents the prevalence of Neisseria meningitidis carriage with the identification of sero- and genogroups in professional soldiers serving in the Polish Armed Forces. A total of 1246 soldiers from the 10th Armored Cavalry Brigade in Świętoszów, Poland were examined in the period January-February 2016. Microbiological tests were performed using standard methods (culture, incubation, microscopy, biochemical, and automated identification with VITEK cards). Neisseria meningitidis isolates from carriers were subjected to a slide agglutination test for the identification of serogroups, next bacterial DNA was isolated and genogroups were identified based on the results of PCR...
October 14, 2016: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/27723450/fully-automated-clinical-grade-bone-marrow-processing-a-single-centre-experience
#8
Benedetta Mazzanti, Serena Urbani, Simone Dal Pozzo, Paola Bufano, Lara Ballerini, Alessia Gelli, Irene Sodi, Irene Donnini, Massimo Di Gioia, Stefano Guidi, Julien Camisani, Riccardo Saccardi
BACKGROUND: Clinical grade processing of harvested bone marrow is required in various clinical situations, particularly in the management of ABO mismatching in allogeneic haematopoietic stem cell transplantation (HSCT) and in regenerative medicine. MATERIAL AND METHODS: We report a single-centre experience using a fully automated, clinical grade, closed system (Sepax, Biosafe, Switzerland). From 2003 to 2015, 125 procedures were performed in our laboratory, including buffy-coat production for HSCT (n=58), regenerative medicine in an orthopaedic setting (n=54) and density-gradient separation in a trial for treatment of critical limb ischaemia (n=13)...
September 27, 2016: Blood Transfusion, Trasfusione del Sangue
https://www.readbyqxmd.com/read/27716472/efficiency-of-a-cleaning-protocol-for-the-removal-of-enterotoxigenic-staphylococcus-aureus-strains-in-dairy-plants
#9
José Guilherme Prado Martin, Gabriela de Oliveira E Silva, Carolina Rodrigues da Fonseca, Caio Baptista Morales, Caroline Souza Pamplona Silva, Daniel Lima Miquelluti, Ernani Porto
Staphylococci are considered a major concern in dairy plants mainly due to the intensive production flow, automation of processing plants and increased demand in the microbiological quality of dairy products. This study aimed to identify S. aureus strains isolated from three Brazilian dairy plants, evaluate the influence of time, temperature and contact surface on the bacterial adhesion process, as well as the efficiency of simulated hygiene and sanitation protocol in removing adhered cells. For genotypic analyses, the presence of icaA and icaD in strains was evaluated...
December 5, 2016: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/27697104/validation-of-the-applied-biosystems-rapidfinder-shiga-toxin-producing-e-coli-stec-detection-workflow
#10
Sharon Matheny, Robert Tebbs, Erin Crowley, David Goins, Angelia Burrell, Patrick Bird, Michelle Swimley, M Joseph Benzinger, Jonathan Flannery, Jonathan Cloke, James Agin, Benjamin Bastin
The Applied Biosystems™ RapidFinder™ STEC Detection Workflow (Thermo Fisher Scientific) is a complete protocol for the rapid qualitative detection of Escherichia coli (E. coli) O157:H7 and the "Big 6" non-O157 Shiga-like toxin-producing E. coli (STEC) serotypes (defined as serogroups: O26, O45, O103, O111, O121, and O145). The RapidFinder STEC Detection Workflow makes use of either the automated preparation of PCR-ready DNA using the Applied Biosystems PrepSEQ™ Nucleic Acid Extraction Kit in conjunction with the Applied Biosystems MagMAX™ Express 96-well magnetic particle processor or the Applied Biosystems PrepSEQ Rapid Spin kit for manual preparation of PCR-ready DNA...
October 1, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/27683527/interpretation-of-blood-microbiology-results-function-of-the-clinical-microbiologist
#11
Katalin Kristóf, Júlia Pongrácz
The proper use and interpretation of blood microbiology results may be one of the most challenging and one of the most important functions of clinical microbiology laboratories. Effective implementation of this function requires careful consideration of specimen collection and processing, pathogen detection techniques, and prompt and precise reporting of identification and susceptibility results. The responsibility of the treating physician is proper formulation of the analytical request and to provide the laboratory with complete and precise patient information, which are inevitable prerequisites of a proper testing and interpretation...
April 2016: EJIFCC
https://www.readbyqxmd.com/read/27650515/methicillin-resistant-staphylococcus-aureus-isolates-with-sccmec-type-v-and-spa-types-t437-or-t1081-associated-to-discordant-susceptibility-results-between-oxacillin-and-cefoxitin-central-taiwan
#12
Cheng-Mao Ho, Chien-Yu Lin, Mao-Wang Ho, Hsiao-Chuan Lin, Chao-Jung Chen, Lee-Chung Lin, Jang-Jih Lu
Staphylococcus aureus isolates with discordant susceptibility results between oxacillin and cefoxitin obtained using automated microbiology systems are infrequently observed. From April 2013 to December 2014, 1956 methicillin-resistant S. aureus (MRSA) and 1761 methicillin-susceptible S. aureus isolates were obtained from different patients. Forty isolates (1.1% and 2% in case of S. aureus and MRSA, respectively) with discordant susceptibility results (oxacillin susceptible and cefoxitin resistant) and carrying mecA gene were obtained...
August 28, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27633307/multidrug-resistance-acinetobacter-species-at-the-intensive-care-unit-aseer-central-hospital-saudi-arabia-a-one-year-analysis
#13
Ali Al Bshabshe, Martin R P Joseph, Ali Al Hussein, Waleed Haimour, Mohamed E Hamid
OBJECTIVE: To identify and to determine the antimicrobial susceptibility of Acinetobacter baumannii (A. baumannii) clinical isolates from ICU at Aseer Central Hospital. METHODS: The study was conducted in the Intensive Care Unit, Aseer Central Hospital, Saudi Arabia over 13 months period (2014-2015). Acinetobacter species (n = 105) were isolated from various clinical samples. Isolates were identified using selected phenotypic criteria and confirmed using the Vitek 2 automated system...
September 2016: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27630804/emerging-bacterial-infection-identification-and-clinical-significance-of-kocuria-species
#14
REVIEW
Venkataramana Kandi, Padmavali Palange, Ritu Vaish, Adnan Bashir Bhatti, Vinod Kale, Maheshwar Reddy Kandi, Mohan Rao Bhoomagiri
Recently there have been reports of gram-positive cocci which are morphologically similar to both Staphylococci and the Micrococci. These bacteria have been identified as Kocuria species with the help of automated identification system and other molecular methods including 16S rRNA (ribosomal ribonucleic acid) evaluation. Kocuria belongs to the family Micrococcaceae which also includes Staphylococcus species and Micrococcus species. Isolation and clinical significance of these bacteria from human specimens warrant great caution as it does not necessarily confirm infection due to their ubiquitous presence, and as a normal flora of skin and mucous membranes in human and animals...
2016: Curēus
https://www.readbyqxmd.com/read/27598407/mass-spectrometry-applications-in-microbiology-beyond-microbe-identification-progress-and-potential
#15
Maurizio Sanguinetti, Brunella Posteraro
INTRODUCTION: Mass spectrometry (MS), particularly MALDI-time of flight (MALDI-TOF), has become a routine tool for microorganism identification in clinical microbiology laboratories in the last five years. The use of MALDI-TOF MS has accelerated laboratory analysis, thus providing accurate species-level information with very short turnaround times. AREAS COVERED: Beyond microbe identification, MALDI-TOF MS offers great opportunities for fast strain typing and detection of antimicrobial susceptibility/resistance in both bacterial and fungal organisms...
September 6, 2016: Expert Review of Proteomics
https://www.readbyqxmd.com/read/27588292/hygiene-the-looming-achilles-heel-in-endoscopy
#16
REVIEW
Michael Jung, Ulrike Beilenhoff
BACKGROUND: Since the late 1970s there have been sporadic reports of nosocomial infections linked to endoscopic procedures. Infections by multidrug-resistant organisms (MDRO) have an increasing impact on healthcare systems worldwide. Since 2010 outbreaks involving MDRO have been reported as a result of endoscopic retrograde cholangiopancreatography (ERCP) from the USA, France, Germany and the Netherlands. METHODS: This article evaluates the recent outbreaks and developments and demonstrates a structural approach to how to prevent future infections...
February 2016: Visceral Medicine
https://www.readbyqxmd.com/read/27566474/culture-medium-optimization-for-osmotolerant-yeasts-by-use-of-a-parallel-fermenter-system-and-rapid-microbiological-testing
#17
Jens Pfannebecker, Claudia Schiffer-Hetz, Jürgen Fröhlich, Barbara Becker
In the present study, a culture medium for qualitative detection of osmotolerant yeasts, named OM, was developed. For the development, culture media with different concentrations of glucose, fructose, potassium chloride and glycerin were analyzed in a Biolumix™ test incubator. Selectivity for osmotolerant yeasts was guaranteed by a water activity (aw)-value of 0.91. The best results regarding fast growth of Zygosaccharomyces rouxii (WH 1002) were achieved in a culture medium consisting of 45% glucose, 5% fructose and 0...
November 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27560299/automated-high-throughput-identification-and-characterization-of-clinically-important-bacteria-and-fungi-using-rapid-evaporative-ionization-mass-spectrometry
#18
Frances Bolt, Simon J S Cameron, Tamas Karancsi, Daniel Simon, Richard Schaffer, Tony Rickards, Kate Hardiman, Adam Burke, Zsolt Bodai, Alvaro Perdones-Montero, Monica Rebec, Julia Balog, Zoltan Takats
Rapid evaporative ionization mass spectrometry (REIMS) has been shown to quickly and accurately speciate microorganisms based upon their species-specific lipid profile. Previous work by members of this group showed that the use of a hand-held bipolar probe allowed REIMS to analyze microbial cultures directly from culture plates without any prior preparation. However, this method of analysis would likely be unsuitable for a high-throughput clinical microbiology laboratory. Here, we report the creation of a customized platform that enables automated, high-throughput REIMS analysis that requires minimal user input and operation and is suitable for use in clinical microbiology laboratories...
October 4, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27543377/comparison-of-manual-and-automated-nucleic-acid-extraction-methods-from-clinical-specimens-for-microbial-diagnosis-purposes
#19
Aniela Wozniak, Enrique Geoffroy, Carolina Miranda, Claudia Castillo, Francia Sanhueza, Patricia García
The choice of nucleic acids (NAs) extraction method for molecular diagnosis in microbiology is of major importance because of the low microbial load, different nature of microorganisms, and clinical specimens. The NA yield of different extraction methods has been mostly studied using spiked samples. However, information from real human clinical specimens is scarce. The purpose of this study was to compare the performance of a manual low-cost extraction method (Qiagen kit or salting-out extraction method) with the automated high-cost MagNAPure Compact method...
November 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27510833/automatic-digital-plate-reading-for-surveillance-cultures
#20
Thomas J Kirn
The automation of specimen processing and culture workup has rapidly emerged in clinical microbiology laboratories throughout the world and more recently in the United States. While many U.S. laboratories have implemented some form of automated specimen processing and some have begun performing digital plate reading, automated colony analysis is just beginning to be utilized clinically. In this issue of the Journal of Clinical Microbiology, M. L. Faron et al. (J Clin Microbiol 54:2470-2475, 2016, http://dx...
October 2016: Journal of Clinical Microbiology
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