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https://www.readbyqxmd.com/read/28700901/hyperspectral-image-analysis-for-rapid-and-accurate-discrimination-of-bacterial-infections-a-benchmark-study
#1
Simone Arrigoni, Giovanni Turra, Alberto Signoroni
With the rapid diffusion of Full Laboratory Automation systems, Clinical Microbiology is currently experiencing a new digital revolution. The ability to capture and process large amounts of visual data from microbiological specimen processing enables the definition of completely new objectives. These include the direct identification of pathogens growing on culturing plates, with expected improvements in rapid definition of the right treatment for patients affected by bacterial infections. In this framework, the synergies between light spectroscopy and image analysis, offered by hyperspectral imaging, are of prominent interest...
June 21, 2017: Computers in Biology and Medicine
https://www.readbyqxmd.com/read/28689506/description-and-validation-of-a-new-automated-surveillance-system-for-clostridium-difficile-in-denmark
#2
M Chaine, S Gubbels, M Voldstedlund, B Kristensen, J Nielsen, L P Andersen, S Ellermann-Eriksen, J Engberg, A Holm, B Olesen, H C Schønheyder, C Østergaard, S Ethelberg, K Mølbak
The surveillance of Clostridium difficile (CD) in Denmark consists of laboratory based data from Departments of Clinical Microbiology (DCMs) sent to the National Registry of Enteric Pathogens (NREP). We validated a new surveillance system for CD based on the Danish Microbiology Database (MiBa). MiBa automatically collects microbiological test results from all Danish DCMs. We built an algorithm to identify positive test results for CD recorded in MiBa. A CD case was defined as a person with a positive culture for CD or PCR detection of toxin A and/or B and/or binary toxin...
July 10, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28687186/evaluation-of-a-new-packaging-process-for-non-autoclavable-endoscopes-results-for-the-first-100-microbiological-samples
#3
C Minebois, P Saviuc, J Shum, P Tuvignon, I Pelloux, M-P Brenier-Pinchart, C Landelle, M-R Mallaret
BACKGROUND: A new process for packaging endoscopes (SureStore(®), Medical Innovations Group) immediately after they exit from washing and disinfection in an automated endoscope reprocessor (AER) allows for endoscopes to be stored for up to 15 days. AIM: To describe the microbiological quality of samples from gastrointestinal endoscopes following this process. METHODS: Three-month prospective study using microbiological sampling from a stock of 38 gastrointestinal endoscopes carried out in a French University Hospital...
July 4, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28680970/nbiochip-a-lab-on-a-chip-platform-of-mono-and-polymicrobial-biofilms-for-high-throughput-downstream-applications
#4
Anand Srinivasan, Nelson S Torres, Kai P Leung, Jose L Lopez-Ribot, Anand K Ramasubramanian
Current in vitro techniques for the culture of microorganisms, and particularly of delicate microbial biofilms, are still mostly limited to low-density plates and manual labor and are not amenable to automation and true high-throughput (HT) applications. We have developed a novel fully automated platform for the formation of mono- and polymicrobial biofilms of Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans at the nanoscale level. The nBioChip is robotically printed, robustly handled, and scanned using a standard microarray reader...
May 2017: MSphere
https://www.readbyqxmd.com/read/28657470/point-of-care-adipose-derived-stromal-vascular-fraction-cell-isolation-and-eptfe-graft-sodding
#5
Stuart K Williams, Marvin E Morris, Paul Kosnik, Kevin D Lye, Gary Gentzkow, Charles B Ross, Amit J Dwivedi, Leigh B Kleinert
Adipose-derived stromal vascular fraction cell populations are being evaluated for numerous clinical applications. The current study evaluated a point of care (POC) technology, the Tissue Genesis "TGI 1000" Cell Isolation System™, to perform an automated isolation of adipose-derived stromal vascular fraction cells to be used in the fabrication of a tissue engineered vascular graft in the operating room. A total of 7 patients were enrolled in this FDA registered Investigational Device Exemption clinical trial and received femoral to tibial expanded polytetrafluoroethylene (ePTFE) bypass grafts to treat peripheral arterial disease...
June 28, 2017: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/28657416/maldi-tof-mass-spectrometry-provides-high-accuracy-in-identification-of-salmonella-at-species-level-but-is-limited-to-type-or-subtype-salmonella-serovars
#6
Lin Kang, Nan Li, Ping Li, Yang Zhou, Shan Gao, Hongwei Gao, Wenwen Xin, Jinglin Wang
Salmonella can cause global foodborne illnesses in humans and many animals. The current diagnostic gold standard used for detecting Salmonella infection is microbiological culture followed by serological confirmation tests. However, these methods are complicated and time-consuming. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis offers some advantages in rapid identification, for example, simple and fast sample preparation, fast and automated measurement, and robust and reliable identification up to genus and species levels, possibly even to the strain level...
April 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/28651665/using-milk-leukocyte-differentials-for-diagnosis-of-subclinical-bovine-mastitis
#7
Juliano Leonel Gonçalves, Roberta L Lyman, Mitchell Hockett, Rudy Rodriguez, Marcos Veiga Dos Santos, Kevin L Anderson
This research study aimed to evaluate the use of the milk leukocyte differential (MLD) to: (a) identify quarter milks that are culture-positive; and (b) characterize the milk leukocyte responses to specific groups of pathogens causing subclinical mastitis. The MLD measures the absolute number and relative percentage of inflammatory cells in milk samples. Using the MLD in two dairy herds (170 and 172 lactating cows, respectively), we studied all lactating cows with a most recent monthly Dairy Herd Improvement Association somatic cell count (SCC) >200 × 103 cells/ml...
June 27, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28638468/current-and-emerging-techniques-for-antibiotic-susceptibility-tests
#8
REVIEW
Karan Syal, Manni Mo, Hui Yu, Rafael Iriya, Wenwen Jing, Sui Guodong, Shaopeng Wang, Thomas E Grys, Shelley E Haydel, Nongjian Tao
Infectious diseases caused by bacterial pathogens are a worldwide burden. Serious bacterial infection-related complications, such as sepsis, affect over a million people every year with mortality rates ranging from 30% to 50%. Crucial clinical microbiology laboratory responsibilities associated with patient management and treatment include isolating and identifying the causative bacterium and performing antibiotic susceptibility tests (ASTs), which are labor-intensive, complex, imprecise, and slow (taking days, depending on the growth rate of the pathogen)...
2017: Theranostics
https://www.readbyqxmd.com/read/28625155/applications-of-optical-dna-mapping-in-microbiology
#9
Diana Bogas, Lena Nyberg, Rui Pacheco, Nuno F Azevedo, Jason P Beech, Margarita Gomila, Jorge Lalucat, Célia M Manaia, Olga C Nunes, Jonas O Tegenfeldt, Fredrik Westerlund
Optical mapping (OM) has been used in microbiology for the past 20 years, initially as a technique to facilitate DNA sequence-based studies; however, with decreases in DNA sequencing costs and increases in sequence output from automated sequencing platforms, OM has grown into an important auxiliary tool for genome assembly and comparison. Currently, there are a number of new and exciting applications for OM in the field of microbiology, including investigation of disease outbreaks, identification of specific genes of clinical and/or epidemiological relevance, and the possibility of single-cell analysis when combined with cell-sorting approaches...
June 1, 2017: BioTechniques
https://www.readbyqxmd.com/read/28566082/-femoral-hemodialysis-catheter-related-bacteremia-due-to-globicatella-sanguinis-challenges-in-species-identification
#10
Elif Aktaş, Nafia Canan Gürsoy, Tamer Sakacı, Yener Koç, Aziz Ahmad Hamidi, Emin Bulut, Duygu Erdemir, Barış Otlu
In this case, catheter-related bacteremia due to Globicatella sanguinis in a 43 years old female patient undergoing hemodialysis with the diagnosis of diabetic nephropathy was presented and the methods in the laboratory diagnosis of the rare opportunistic pathogen, Globicatella cins, were nvestigated. A set of peripheral blood cultures and simultaneous catheter culture was obtained from the patient in third of May 2016. Biochemical tests, Phoenix (Becton Dickinson, USA) and MicroScan (Beckman Coulter, USA) automated systems and matrix assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF MS) based Microflex MS (Bruker, Daltonics, Germany) and VITEK MS (database v2...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28566080/-molecular-epidemiology-of-human-mycobacterium-bovis-infection-in-aegean-region-turkey
#11
Cengiz Çavuşoğlu, Fethiye Ferda Yılmaz
Mycobacterium bovis, which has a broader host spectrum as opposed to Mycobacterium tuberculosis that generally causes disease in humans, mainly leads to chronic progressive pulmonary disease in a large number of domestic and wild mammals, particularly cattle. The term "zoonotic tuberculosis (TB)" is used to describe M.bovis infection in humans. "Zoonotic TB" can not be differentiated clinically, radiologically or pathologically from TB caused by M.tuberculosis. The aim of this study was to evaluate the role of M...
April 2017: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28559740/the-anti-staphylococcus-aureus-effect-of-combined-echinophora-platyloba-essential-oil-and-liquid-smoke-in-beef
#12
Zahra Pilevar, Hedayat Hosseini, Homa Hajimehdipoor, Farzaneh Shahraz, Leyla Alizadeh, Amin Mousavi Khaneghah, Maryam Mahmoudzadeh
In the current study, the antibacterial effect of Echinophora platyloba essential oil and common liquid smoke (individually and in combination) against Staphylococcus aureus in beef meat samples is investigated. Using an automated microbiological growth analyser and the turbidimetric technique, the minimum inhibitory concentrations (MIC) and the minimum bactericidal concentrations (MBC) of the essential oil and liquid smoke were determined. Anti-S. aureus activity of essential oil and liquid smoke (individually and in combination) was defined by disk diffusion assay, generation time and cell constituent release...
March 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28559002/assessment-of-the-automated-multiplex-pcr-unyvero-i60-iti-%C3%A2-cartridge-system-to-diagnose-prosthetic-joint-infection-a-multicenter-study
#13
D Malandain, P Bémer, A G Leroy, J Léger, C Plouzeau, A S Valentin, A Jolivet-Gougeon, D Tandé, G Héry-Arnaud, C Lemarié, M Kempf, L Bret, C Burucoa, S Corvec
OBJECTIVES: Prosthetic joint infections (PJI) are responsible for significant morbidity and mortality and their number continues to rise. Their management remains complex, especially the microbiological diagnosis. Besides "homemade" tests developed by several teams, new molecular biology methods are now available with different analytical performance and usability. METHODS: We studied the performances of one of these tests: ITI(®) multiplex PCR (mPCR) by the Curetis(®) company and compared it either to "optimized" culture or to 16S rRNA PCR...
May 27, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28460559/discrimination-of-streptococcus-equi-subsp-equi-and-streptococcus-equi-subsp-zooepidemicus-using-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry
#14
Rinosh J Mani, Anil J Thachil, Akhilesh Ramachandran
Accurate and timely identification of infectious etiologies is of great significance in veterinary microbiology, especially for critical diseases such as strangles, a highly contagious disease of horses caused by Streptococcus equi subsp. equi. We evaluated a matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) platform for use in species- and subspecies-level identification of S. equi isolates from horses and compared it with an automated biochemical system. We used 25 clinical isolates each of S...
April 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28455579/-new-microbiological-techniques
#15
REVIEW
S Schubert, A Wieser, G Bonkat
Microbiological diagnostic procedures have changed rapidly in recent years. This is especially true in the field of molecular diagnostics. Classical culture-based techniques are still the gold standard in many areas; however, they are already complemented by automated and also molecular techniques to guarantee faster and better quality results. The most commonly used techniques include real-time polymerase chain reaction (RT-PCR) based systems and nucleic acid hybridization. These procedures are used most powerfully from direct patient samples or in assays to detect the presence of nonculturable or fastidious organisms...
April 28, 2017: Der Urologe. Ausg. A
https://www.readbyqxmd.com/read/28412378/project-management-importance-for-diagnostic-laboratories
#16
REVIEW
A Croxatto, G Greub
BACKGROUND: The need for diagnostic laboratories to improve both quality and productivity alongside personnel shortages incite laboratory managers to constantly optimize laboratory workflows, organization, and technology. These continuous modifications of the laboratories should be conducted using efficient project and change management approaches to maximize the opportunities for successful completion of the project. AIM: This review aims at presenting a general overview of project management with an emphasis on selected critical aspects...
July 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28409124/hospital-dissemination-of-tst-1-positive-clonal-complex-5-cc5-methicillin-resistant-staphylococcus-aureus
#17
Min Wang, Yi Zheng, Jose R Mediavilla, Liang Chen, Barry N Kreiswirth, Yajun Song, Ruifu Yang, Hong Du
Methicillin-resistant Staphylococcus aureus (MRSA), is one of the most prevalent clinical pathogens isolated from hospital settings, and has increasingly identified in community settings. In China, the SCCmecIII-ST239 strains are disseminated in different geographic regions, accounting for >75% of all MRSA isolates in some national studies. Here we characterized 150 non-duplicate MRSA isolates collected from February 2012 to May 2013 in a tertiary hospital in Suzhou, Eastern China, to explore the molecular epidemiology...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28402209/culturomics-a-new-kid-on-the-block-of-omics-to-enable-personalized-medicine
#18
Manousos E Kambouris, Cristiana Pavlidis, Efthymios Skoufas, Michael Arabatzis, Maria Kantzanou, Aristea Velegraki, George P Patrinos
This innovation analysis highlights the underestimated and versatile potential of the new field of culturomics and examines its relation to other OMICS system sciences such as infectiomics, metabolomics, phenomics, and pharmacomicrobiomics. The advent of molecular biology, followed by the emergence of various disciplines of the genomics, and most importantly metagenomics, brought about the sharp decline of conventional microbiology methods. Emergence of culturomics has a natural synergy with therapeutic and clinical genomic approaches so as to realize personalized medicine...
April 12, 2017: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/28397472/comparison-of-novel-blood-culture-system-dl-bt112tm-with-bact-alert-3dtm-by-using-clinical-samples
#19
Mustafa Altindis, Tayfur Demiray, Mehmet Koroglu, Umit Kilic, Aybala Neslihan Alagoz, Aziz Ogutlu, Oguz Karabay
BACKGROUND: Blood cultures are the main diagnostic laboratory tool to detect bloodstream infections. Many clinical microbiology laboratories utilize automated blood culture systems to isolate infectious agents from blood samples. The diagnostic performance and time-to-detection values of the novel automated blood culture system, DLBt112TM (DL), was compared with BacT/Alert 3DTM (B3D) in this prospective comparative study with clinical samples. METHODS: A total of 356 blood culture sets (178 sets for each system) were evaluated over a 6-month period in a university hospital...
April 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28391814/antibiogramj-a-tool-for-analysing-images-from-disk-diffusion-tests
#20
C A Alonso, C Domínguez, J Heras, E Mata, V Pascual, C Torres, M Zarazaga
BACKGROUND AND OBJECTIVES: Disk diffusion testing, known as antibiogram, is widely applied in microbiology to determine the antimicrobial susceptibility of microorganisms. The measurement of the diameter of the zone of growth inhibition of microorganisms around the antimicrobial disks in the antibiogram is frequently performed manually by specialists using a ruler. This is a time-consuming and error-prone task that might be simplified using automated or semi-automated inhibition zone readers...
May 2017: Computer Methods and Programs in Biomedicine
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