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Microbiology automation

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https://www.readbyqxmd.com/read/28222388/microbial-profile-of-dental-plaque-associated-to-white-spot-lesions-in-orthodontic-patients-immediately-after-the-bracket-removal
#1
Moniek W Beerens, Jacob M Ten Cate, Monique H van der Veen
OBJECTIVE: Denaturing Gradient Gel Electrophoresis (DGGE) is suggested to predict caries risk in young children. Such a tool would be valuable in orthodontic patients undergoing treatment with fixed appliances. In this cross-sectional study the applicability of DGGE and conventional microbiology for caries risk assessment in orthodontic patients were assessed. DESIGN: Dental plaque was obtained from orthodontic patients immediately prior to bracket removal. Presence of white spot lesions (WSL) was assessed immediately post debracketing...
February 9, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/28214393/flow-cytometric-bacterial-cell-counts-challenge-conventional-heterotrophic-plate-counts-for-routine-microbiological-drinking-water-monitoring
#2
REVIEW
S Van Nevel, S Koetzsch, C R Proctor, M D Besmer, E I Prest, J S Vrouwenvelder, A Knezev, N Boon, F Hammes
Drinking water utilities and researchers continue to rely on the century-old heterotrophic plate counts (HPC) method for routine assessment of general microbiological water quality. Bacterial cell counting with flow cytometry (FCM) is one of a number of alternative methods that challenge this status quo and provide an opportunity for improved water quality monitoring. After more than a decade of application in drinking water research, FCM methodology is optimised and established for routine application, supported by a considerable amount of data from multiple full-scale studies...
February 8, 2017: Water Research
https://www.readbyqxmd.com/read/28164570/time-to-detection-comparison-for-a-novel-blood-culture-system-using-simulated-blood-cultures-dltm-versus-bact-alerttm-and-bactectm
#3
Tayfur Demiray, Mehmet Koroglu, Mustafa Altindis
BACKGROUND: Automated blood culture systems are routinely used in microbiology laboratories to isolate bacteria and fungi causing bloodstream infections. A novel automated blood culture system, DL-Bt112TM (DL) was compared with the BACTEC 9050TM (BCT) and BacT/Alert 3DTM (B3D) systems for time-to-detection (TTD) using 10 different clinical bacteria that commonly cause bloodstream infections. METHODS: Simulated blood cultures were used to compare the three automated blood culture systems...
September 1, 2016: Clinical Laboratory
https://www.readbyqxmd.com/read/28129707/fluorescence-in-situ-hybridization-fish-in-the-microbiological-diagnostic-routine-laboratory-a-review
#4
Hagen Frickmann, Andreas Erich Zautner, Annette Moter, Judith Kikhney, Ralf Matthias Hagen, Henrik Stender, Sven Poppert
Early identification of microbial pathogens is essential for rational and conservative antibiotic use especially in the case of known regional resistance patterns. Here, we describe fluorescence in situ hybridization (FISH) as one of the rapid methods for easy identification of microbial pathogens, and its advantages and disadvantages for the diagnosis of pathogens in human infections in the laboratory diagnostic routine. Binding of short fluorescence-labeled DNA or nucleic acid-mimicking PNA probes to ribosomes of infectious agents with consecutive analysis by fluorescence microscopy allows identification of bacterial and eukaryotic pathogens at genus or species level...
January 27, 2017: Critical Reviews in Microbiology
https://www.readbyqxmd.com/read/28124968/-accurate-diagnosis-of-pseudomonas-luteola-in-routine-microbiology-laboratory-on-the-occasion-of-two-isolates
#5
Muharrem Çiçek, Gülşen Hasçelik, H Kaan Müştak, K Serdar Diker, Burçin Şener
Pseudomonas luteola which was previously known as Chryseomonas luteola; is a gram-negative, non-fermentative, aerobic, motile, non-spore-forming bacillus. It is frequently found as a saprophyte in soil, water and other damp environments and is an opportunistic pathogen in patients with underlying medical disorders or with indwelling catheters. It has been reported as an uncommon cause of bacteremia, sepsis, septic arthritis, meningitis, endocarditis, and peritonitis. Thus, early and accurate identification of this rare species is important for the treatment and also to provide information about the epidemiology of P...
October 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/28122803/a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#6
REVIEW
John D Perry
In the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. In the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including Pseudomonas aeruginosa, group B streptococci, Clostridium difficile, Campylobacter spp., and Yersinia enterocolitica. New media have also been developed to screen for pathogens with acquired antimicrobial resistance, including vancomycin-resistant enterococci, carbapenem-resistant Acinetobacter spp...
April 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28087144/-contribution-of-microbiology-in-the-diagnosis-of-tuberculosis-in-castile-and-le%C3%A3-n-spain-findings-of-the-grumicale-2013-study
#7
Ramiro López-Medrano, Teresa Nebreda-Mayoral, M Fé Brezmes-Valdivieso, Susana García-de Cruz, Begoña Nogueira-González, Rafael Sánchez-Arroyo, Almudena Tinajas-Puertas, Nieves Gutiérrez-Zufiaurre, Cristina Labayru-Echeverría, Susana Hernando-Real, Luis López-Urrutia, Octavio Rivero-Lezcano, Belén Ullivarri-Francia, Raquel Rodríguez-Tarazona, Isabel Antolín-Ayala
INTRODUCTION AND OBJECTIVES: A retrospective study was conducted by collecting microbiological tuberculosis (TB) data in Castile and León during the year 2013 in order to determine the incidence and distribution of TB, and resistance to the tuberculostatic drug, and compare them with the epidemiological data provided by the Department of Epidemiological Surveillance (SIVE). MATERIAL AND METHODS: Microbiologists of the 14 hospitals of the Castile and León public health network (GRUMICALE) collected epidemiological, microbiological, and management data from the Microbiology laboratories in the community during the year 2013...
January 10, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28068884/evaluation-of-protein-spectra-cluster-analysis-for-streptococcus-spp-identification-from-various-swine-clinical-samples
#8
Carlos E C Matajira, Luisa Z Moreno, Vasco T M Gomes, Ana Paula S Silva, Renan E Mesquita, Daniela S Doto, Franco F Calderaro, Fernando N de Souza, Ana Paula G Christ, Maria Inês Z Sato, Andrea M Moreno
Traditional microbiological methods enable genus-level identification of Streptococcus spp. isolates. However, as the species of this genus show broad phenotypic variation, species-level identification or even differentiation within the genus is difficult. Herein we report the evaluation of protein spectra cluster analysis for the identification of Streptococcus species associated with disease in swine by means of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). A total of 250 S...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28067541/listeria-monocytogenes-circulating-in-rabbit-meat-products-and-slaughterhouses-in-italy-prevalence-data-and-comparison-among-typing-results
#9
Alessandra De Cesare, Antonio Parisi, Renzo Mioni, Damiano Comin, Alex Lucchi, Gerardo Manfreda
Rabbit meat has outstanding dietetic and nutritional properties. However, few data on microbiological hazards associated with rabbit productions are available. In this study, the presence of Listeria monocytogenes was determined in 430 rabbit carcasses, 256 rabbit meat cuts and products, and 599 environmental sponges collected from four Italian rabbit slaughterhouses over a period of 1 year. Prevalence of L. monocytogenes among the 1285 rabbit meat and environmental samples was 11%, with statistically significant differences between slaughterhouses...
January 9, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28066816/from-genomes-to-phenotypes-traitar-the-microbial-trait-analyzer
#10
Aaron Weimann, Kyra Mooren, Jeremy Frank, Phillip B Pope, Andreas Bremges, Alice C McHardy
The number of sequenced genomes is growing exponentially, profoundly shifting the bottleneck from data generation to genome interpretation. Traits are often used to characterize and distinguish bacteria and are likely a driving factor in microbial community composition, yet little is known about the traits of most microbes. We describe Traitar, the microbial trait analyzer, which is a fully automated software package for deriving phenotypes from a genome sequence. Traitar provides phenotype classifiers to predict 67 traits related to the use of various substrates as carbon and energy sources, oxygen requirement, morphology, antibiotic susceptibility, proteolysis, and enzymatic activities...
November 2016: MSystems
https://www.readbyqxmd.com/read/28053214/the-bactec-fx-blood-culture-system-detects-brucella-melitensis-bacteremia-in-adult-patients-within-the-routine-one-week-incubation-period
#11
Moshe Sagi, Lior Nesher, Pablo Yagupsky
The performance of the BACTEC FX blood culture system for detecting Brucella bacteremia within the routine one-week incubation period was assessed in a prospective study conducted in an endemic area for B. melitensis in southern Israel. Aerobic vials (BD BACTEC™ Plus Aerobic/F Medium) inoculated with blood specimens obtained from adult patients with a positive Rose-Bengal screening test were monitored for four consecutive weeks and blind subcultures of negative vials were performed on solid media on days 7 and 28...
January 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28043600/benefits-and-challenges-of-molecular-diagnostics-for-childhood-tuberculosis
#12
Cristina Gutierrez
Expanding tuberculosis (TB)-diagnostic services, including access to rapid tests, is a World Health Organization (WHO) strategy to accelerate progress toward ending TB. Faster and more sensitive molecular tests capable of diagnosing TB and drug-resistant TB have the technical capacity to address limitations associated with smears and cultures by increasing accuracy and shortening turnaround times as compared with those of these conventional laboratory methods. Nucleic acid amplification assays used to detect and analyze Mycobacterium tuberculosis (MTB)-complex nucleic acids can be used directly on specimens from patients suspected of having TB...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28003626/methods-of-rapid-microbiological-assay-and-their-application-to-pharmaceutical-and-medical-device-fabrication
#13
REVIEW
Hideharu Shintani
 There are several rapid microbiological methods becoming available that have useful applications in pharmaceutical and medical devices. They are ATP bioluminescence, fluorescent labeling, electrical resistance, and nucleic acid probes. In choosing to employ rapid methods, the microbiologist should examine their prospective performances against the specific requirements for that sector. Some methods may require expensive equipment and offer full automation, and others represent only a small investment. The regulatory view of these methods is changing and they still officially have not been approved in medical and pharmaceutical area, but it will still be up to the microbiologist to demonstrate that the method chosen is fit for the purpose intended...
2016: Biocontrol Science
https://www.readbyqxmd.com/read/27993473/evaluation-of-%C3%AE-d-glucuronidase-and-particle-size-distribution-for-microbiological-water-quality-monitoring-in-northern-vietnam
#14
Anna Ender, Nadine Goeppert, Felix Grimmeisen, Nico Goldscheider
In many karst regions in developing countries, the populations often suffer from poor microbial water quality and are frequently exposed to bacterial pathogens. The high variability of water quality requires rapid assays, but the conventional cultivation-based analysis of fecal indicator bacteria, such as Escherichia coli (E. coli), is very time-consuming. In this respect, the measurement of the enzymatic activity of E. coli could prove to be a valuable tool for water quality monitoring. A mobile automated prototype device was used for the investigation of β-d-glucuronidase (GLUC) activity at a remote karst spring, connected to a sinking surface stream, in Northern Vietnam...
December 16, 2016: Science of the Total Environment
https://www.readbyqxmd.com/read/27955641/microbial-phenomics-information-extractor-micropie-a-natural-language-processing-tool-for-the-automated-acquisition-of-prokaryotic-phenotypic-characters-from-text-sources
#15
Jin Mao, Lisa R Moore, Carrine E Blank, Elvis Hsin-Hui Wu, Marcia Ackerman, Sonali Ranade, Hong Cui
BACKGROUND: The large-scale analysis of phenomic data (i.e., full phenotypic traits of an organism, such as shape, metabolic substrates, and growth conditions) in microbial bioinformatics has been hampered by the lack of tools to rapidly and accurately extract phenotypic data from existing legacy text in the field of microbiology. To quickly obtain knowledge on the distribution and evolution of microbial traits, an information extraction system needed to be developed to extract phenotypic characters from large numbers of taxonomic descriptions so they can be used as input to existing phylogenetic analysis software packages...
December 13, 2016: BMC Bioinformatics
https://www.readbyqxmd.com/read/27837517/microarray-technologies-in-fungal-diagnostics
#16
Steffen Rupp
Microarray technologies have been a major research tool in the last decades. In addition they have been introduced into several fields of diagnostics including diagnostics of infectious diseases. Microarrays are highly parallelized assay systems that initially were developed for multiparametric nucleic acid detection. From there on they rapidly developed towards a tool for the detection of all kind of biological compounds (DNA, RNA, proteins, cells, nucleic acids, carbohydrates, etc.) or their modifications (methylation, phosphorylation, etc...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27805525/microbiologic-investigation-of-patients-before-living-donor-liver-transplant
#17
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Chronic infections can cause serious complications and create morbidity and mortality for affected patients during immunosuppressive therapy in the posttransplant period. Here, we studied the microbiologic screening results of patients before living-donor liver transplant. MATERIALS AND METHODS: The microbiologic screening results of 25 patients during 2015 at National Scientific Research Center (Astana, Kazakhstan) before livingdonor liver transplant were prospectively analyzed...
2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27805513/microbiologic-monitoring-of-kidney-transplant-recipients-before-transplant
#18
Nelya Bissenova, Aigerim Yergaliyeva
OBJECTIVES: Drug immunosuppression, traditionally used to minimize autoimmune developments after liver, kidney, heart, and lung transplant, and which requires high doses of corticosteroids, creates favorable conditions for the development of various infectious processes and the existence of pathogens, which can be activated after transplant. This study aimed to investigate the bacterial pathogens in patients before living-donor kidney transplant. MATERIALS AND METHODS: We prospectively investigated 117 clinical samples of 33 patients who were seen during 2015 at the National Scientific Research Center (Astana, Kazakhstan) before living-donor kidney transplant...
2016: Experimental and Clinical Transplantation
https://www.readbyqxmd.com/read/27801488/peritoneal-dialysis-experience-of-the-department-of-pediatrics-of-the-hospital-charles-nicolle-of-tunis
#19
Manel Jellouli, Meriem Ferjani, Amal Oueslati, Kamel Abidi, Ouns Naija, Yousra Hammi, Taieb Ben Abdallah, Tahar Gargah
Introduction Peritoneal dialysis (PD) is still the most common modality used in treatment for children with End Stage Renal Disease (ESRD). The objective of this study was to identify the epidemiological, clinical, and microbiological factors affecting the outcome of PD. Methods In this study, we retrospectively reviewed the records of 85 patients who were treated with DP for the last ten years (from January 2004 to December 2013) in the Department of Pediatrics in Charles Nicolle hospital, Tunis. Results The mean duration of PD was 18...
May 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/27766214/an-overview-on-novel-microbial-determination-methods-in-pharmaceutical-and-food-quality-control
#20
Mahboob Nemati, Aliasghar Hamidi, Solmaz Maleki Dizaj, Vahid Javaherzadeh, Farzaneh Lotfipour
Traditional microbiological methods tend to be labor-intensive and time-consuming. Rapid and novel methods in microbiological tests provide more sensitive, precise and reproducible results compared with conventional methods. In microbiology, the most rapid testing methods belong to the field of biotechnology such as PCR, ELISA, ATP bioluminescence and etc. Nevertheless impedance microbiology, biosensors and analytical procedures to determine microbial constituents are of significance. The present review article was conducted using internet databases and related scientific literatures and articles that provide information on developments in the rapid methods in microbiology...
September 2016: Advanced Pharmaceutical Bulletin
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