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Microbiology automation

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https://www.readbyqxmd.com/read/29327210/paired-blood-cultures-increase-the-sensitivity-for-detecting-pathogens-in-both-inpatients-and-outpatients
#1
Bansidhar Tarai, Dinesh Jain, Poonam Das, Sandeeep Budhiraja
The objective of this study was to show the differences between paired blood cultures (PBC) versus single blood cultures (SBC) in the microbiologic yield, the sensitivity to detect pathogens and the time to positivity (TTP). We performed a retrospective study examining 112,570 blood culture samples over a 5-year period from July 2011 to May 2016 in the BacT/ALERT® 3D automated blood culture system (bioMérieux, Marcy l'Etoile, France). Bacteria and yeasts were identified using the VITEK® 2 Compact system (bioMérieux, Marcy l'Etoile, France)...
January 11, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29318458/diagnostic-performance-of-blood-culture-bottles-for-vitreous-culture-compared-to-conventional-microbiological-cultures-in-patients-with-suspected-endophthalmitis
#2
Jan Kehrmann, Valerie Chapot, Jan Buer, Philipp Rating, Norbert Bornfeld, Joerg Steinmann
The purpose of this investigation was to evaluate the performance of blood culture bottles in comparison to conventional microbiological culture techniques in detecting causative microorganisms of endophthalmitis and to determine their anti-infective susceptibility profiles. All consecutive cases with clinically suspected endophthalmitis in a university-based ophthalmology department between January 2009 and December 2016 were analysed in this retrospective comparative case series. Samples from 247 patients with suspected endophthalmitis underwent microbiological diagnostic work-up...
January 9, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29314254/manual-versus-automated-streaking-system-in-clinical-microbiology-laboratory-performance-evaluation-of-previ-isola-for-blood-culture-and-body-fluid-samples
#3
Qute Choi, Hyun Jin Kim, Jong Wan Kim, Gye Cheol Kwon, Sun Hoe Koo
BACKGROUND: The process of plate streaking has been automated to improve routine workflow of clinical microbiology laboratories. Although there were many evaluation reports about the inoculation of various body fluid samples, few evaluations have been reported for blood. In this study, we evaluated the performance of automated inoculating system, Previ Isola for various routine clinical samples including blood. METHODS: Blood culture, body fluid, and urine samples were collected...
January 4, 2018: Journal of Clinical Laboratory Analysis
https://www.readbyqxmd.com/read/29310696/methods-for-detecting-gemmata-spp-bacteremia-in-the-microbiology-laboratory
#4
Jacques-Robert Christen, Edwin Edmond, Michel Drancourt
OBJECTIVE: Gemmata bacteria are fastidious, Gram-negative and aerobic. The only representatives are Gemmata obscuriglobus and Gemmata massiliana. These Planctomycetes appear to be a part of human digestive tract microbiome, and G. massiliana has been isolated from water. Further specific detection in the blood of two patients with febrile neutropenia suggests that Gemmata bacteremia may remain under-documented. The objective of this study was to develop an effective protocol to document Gemmata spp...
January 8, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/29305426/rapid-detection-of-periprosthetic-joint-infection-using-a-combination-of-16s-rdna-polymerase-chain-reaction-and-lateral-flow-immunoassay-a-pilot-study
#5
V Janz, J Schoon, C Morgenstern, B Preininger, S Reinke, G Duda, A Breitbach, C F Perka, S Geissler
OBJECTIVES: The objective of this study was to develop a test for the rapid (within 25 minutes) intraoperative detection of bacteria from synovial fluid to diagnose periprosthetic joint infection (PJI). METHODS: The 16s rDNA test combines a polymerase chain reaction (PCR) for amplification of 16s rDNA with a lateral flow immunoassay in one fully automated system. The synovial fluid of 77 patients undergoing joint aspiration or primary or revision total hip or knee surgery was prospectively collected...
January 2018: Bone & Joint Research
https://www.readbyqxmd.com/read/29287094/validation-of-a-novel-automatic-deposition-of-bacteria-and-yeasts-on-maldi-target-for-maldi-tof-ms-based-identification-using-maldi-colonyst-robot
#6
Katerina Chudejova, Michal Bohac, Anna Skalova, Veronika Rotova, Costas C Papagiannitsis, Jana Hanzlickova, Tamara Bergerova, Jaroslav Hrabák
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) -based identification of bacteria and fungi significantly changed the diagnostic process in clinical microbiology. We describe here a novel technique for bacterial and yeast deposition on MALDI target using an automated workflow resulting in an increase of the microbes' score of MALDI identification. We also provide a comparison of four different sample preparation methods. In the first step of the study, 100 Gram-negative bacteria, 100 Gram-positive bacteria, 20 anaerobic bacteria and 20 yeasts were spotted on the MALDI target using manual deposition, semi-extraction, wet deposition onto 70% formic acid and by automatic deposition using MALDI Colonyst...
2017: PloS One
https://www.readbyqxmd.com/read/29284316/profile-of-genmark-s-eplex%C3%A2-blood-culture-identification-fungal-pathogen-panel
#7
Danièle Maubon, Céline Dard, Cécile Garnaud, Muriel Cornet
Fungemia presents high morbi-mortality and thus rapid microbiological diagnosis may contribute to appropriate patient management. In the last decade, kits based on molecular technologies have become available and health care institutes are increasingly facing critical investment choices. Although all these tools aim to achieve rapid fungal detection and species identification, they display different inherent characteristics. Areas covered: Considering technologies allowing detection and identification of fungal species in a sepsis context, the market proposes either tests on positive blood culture or tests on patient's whole blood...
December 28, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/29258740/performance-evaluation-of-bd-phoenix%C3%A2-an-automated-microbiology-system-for-the-screening-of-imp-producing-enterobacteriaceae
#8
Hiromi Yamakawa, Kosuke Kosai, Yasuhide Kawamoto, Norihiko Akamatsu, Junichi Matsuda, Norihito Kaku, Naoki Uno, Yoshitomo Morinaga, Hiroo Hasegawa, Katsunori Yanagihara
BD Phoenix™ is an automated bacterial identification and susceptibility testing system. Here, its performance in screening IMP-producing Enterobacteriaceae was evaluated. The system identified 97.8% of IMP producers as being nonsusceptible to imipenem or meropenem, which was higher than that identified by the broth microdilution method (91.3%, imipenem; 41.3%, meropenem).
December 16, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29242391/method-verification-requirements-for-an-advanced-imaging-system-for-microbial-plate-count-enumeration
#9
David Jones, Tony Cundell
Technical Abstract: The Growth Direct™ System that automates the incubation and reading of membrane filtration microbial counts on soybean-casein digest, Sabouraud dextrose and R2A agar differs only from the traditional method in that micro-colonies on the membrane are counted using an advanced imaging system up to 50% earlier in the incubation. Based on the recommendations in USP <1223> Validation of New Microbiological Testing Methods, the system may be implemented in a microbiology laboratory after simple method verification and not a full method validation...
December 14, 2017: PDA Journal of Pharmaceutical Science and Technology
https://www.readbyqxmd.com/read/29237786/commentary-waltzing-around-sacred-cows-on-the-way-to-the-future
#10
Stephen M Brecher
Our mostly manual, agar based clinical microbiology laboratory is slowly but steadily being re-defined by automation and innovation. Ironically, the oldest test, the Gram stain, is still manually read and interpreted by trained personnel. In this proof of concept study Smith et al. (https://doi.org/10.1128/JCM.01521-17) have used computer imaging with a deep convolutional neural network to examine and interpret Gram-stained slides from positive blood culture bottles. In light of the shortage of medical technologists/microbiologists and the need for results of positive blood culture bottles 24/7, this paper paves the way for the next innovations for the clinical microbiology laboratory of the future...
December 13, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29231688/medical-devices-immunology-and-microbiology-devices-classification-of-the-automated-indirect-immunofluorescence-microscope-and-software-assisted-system-final-order
#11
(no author information available yet)
The Food and Drug Administration (FDA or we) is classifying the automated indirect immunofluorescence microscope and software-assisted system into class II (special controls). The special controls that apply to the device type are identified in this order and will be part of the codified language for the automated indirect immunofluorescence microscope and software-assisted system's classification. We are taking this action because we have determined that classifying the device into class II (special controls) will provide a reasonable assurance of safety and effectiveness of the device...
November 14, 2017: Federal Register
https://www.readbyqxmd.com/read/29228397/yeast-identification-by-sequencing-biochemical-kits-maldi-tof-ms-and-rep-pcr-dna-fingerprinting
#12
Ying Zhao, Chi-Ching Tsang, Meng Xiao, Jasper F W Chan, Susanna K P Lau, Fanrong Kong, Yingchun Xu, Patrick C Y Woo
No study has comprehensively evaluated the performance of 28S nrDNA and ITS sequencing, commercial biochemical test kits, MALDI-TOF MS platforms, and the emerging rep-PCR DNA fingerprinting technology using a cohort of yeast strains collected from a clinical microbiology laboratory. In this study, using 71 clinically important yeast isolates (excluding Candida albicans) collected from a single centre, we determined the concordance of 28S nrDNA and ITS sequencing and evaluated the performance of two commercial test kits, two MALDI-TOF MS platforms, and rep-PCR DNA fingerprinting...
December 8, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29203445/rapid-monitoring-of-local-epidemiology-of-vancomycin-resistant-enterococcus-faecium-on-a-department-level-by-reppcr
#13
Frank Froeschen, Mike Gajdiss, Julia Uebele, Alina Meilaender, Achim Hoerauf, Martin Exner, Ernst Molitor, Gabriele Bierbaum, Steffen Engelhart, Isabelle Bekeredjian-Ding
BACKGROUND: The current increase in nosocomial infections caused by vancomycin-resistant enterococci (VRE) warrants improvement of detection methods and hygiene measures. Knowledge of the local epidemiology is important for monitoring compliance of medical personnel with hygiene measures. AIM: We evaluated semi-automated repPCR for rapid molecular typing of VRE. METHODS: Primary VRE isolates were collected over an observation period of one year and retrospectively typed by repPCR...
December 1, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29187563/automated-interpretation-of-blood-culture-gram-stains-using-a-deep-convolutional-neural-network
#14
Kenneth P Smith, Anthony D Kang, James E Kirby
Microscopic interpretation of stained smears is one of the most operator-dependent and time intensive activities in the clinical microbiology laboratory. Here, we investigated application of an automated image acquisition and convolutional neural network (CNN)-based approach for automated Gram stain classification. Using an automated microscopy platform, uncoverslipped slides were scanned with a 40x dry objective, generating images of sufficient resolution for interpretation. We collected 25,488 images from positive blood culture Gram stains prepared during routine clinical workup...
November 29, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29153182/a-100-year-review-cheese-production-and-quality
#15
M E Johnson
In the beginning, cheese making in the United States was all art, but embracing science and technology was necessary to make progress in producing a higher quality cheese. Traditional cheese making could not keep up with the demand for cheese, and the development of the factory system was necessary. Cheese quality suffered because of poor-quality milk, but 3 major innovations changed that: refrigeration, commercial starters, and the use of pasteurized milk for cheese making. Although by all accounts cold storage improved cheese quality, it was the improvement of milk quality, pasteurization of milk, and the use of reliable cultures for fermentation that had the biggest effect...
December 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/29140977/low-cost-and-open-source-multi-fluorescence-imaging-system-for-teaching-and-research-in-biology-and-bioengineering
#16
Isaac Nuñez, Tamara Matute, Roberto Herrera, Juan Keymer, Timothy Marzullo, Timothy Rudge, Fernán Federici
The advent of easy-to-use open source microcontrollers, off-the-shelf electronics and customizable manufacturing technologies has facilitated the development of inexpensive scientific devices and laboratory equipment. In this study, we describe an imaging system that integrates low-cost and open-source hardware, software and genetic resources. The multi-fluorescence imaging system consists of readily available 470 nm LEDs, a Raspberry Pi camera and a set of filters made with low cost acrylics. This device allows imaging in scales ranging from single colonies to entire plates...
2017: PloS One
https://www.readbyqxmd.com/read/29135992/internal-quality-assurance-in-diagnostic-microbiology-a-simple-approach-for-insightful-data
#17
Valentin Scherz, Christian Durussel, Gilbert Greub
Given the importance of microbiology results on patient care, high quality standards are expected. Internal quality assurance (IQA) could mitigate the limitations of internal quality control, competency assessment and external quality assurance, adding a longitudinal insight, including pre- and post-analytical steps. Here, we implemented an IQA program in our clinical microbiology facilities with blind resubmission of routine samples during 22 months. One-hundred-and-twenty-one out of 123 (98.4%) serological analyses and 112 out of 122 (91...
2017: PloS One
https://www.readbyqxmd.com/read/29118410/the-microbiological-characteristics-and-risk-factors-for-picc-related-bloodstream-infections-in-intensive-care-unit
#18
Shumin Zhang, Xiaofeng Sun, Yan Lei
The study was aimed to investigate the pathogens distribution and risk factors for PICC-related bloodstream infection in intensive care unit (ICU) patients. 402 patients placed with PICC in ICU were recruited in the study. The microbiological characteristics of PICC-related infection were investigated by Vitek 2 Compact automated microbial system. Antibiotics sensitivity was performed with disk diffusion and minimum inhibitory concentration (MIC) methods. Multivariate logistic and cox analyses were performed to identify the risk factors for PICC-related infection in ICU patients...
November 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29118171/total-laboratory-automation-and-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-improve-turnaround-times-in-the-clinical-microbiology-laboratory-a-retrospective-analysis
#19
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and BD Kiestra total laboratory automation (TLA) 4 and 3 years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TATs) for positive and negative urine cultures before and after implementation. In comparison I, TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF MS for organism identification...
January 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/29118168/use-of-accelerate-pheno%C3%A2-system-for-identification-and-antimicrobial-susceptibility-testing-id-ast-of-pathogens-in-positive-blood-cultures-and-impact-on-time-to-results-and-workflow
#20
Angella Charnot-Katsikas, Vera Tesic, Nedra Love, Brandy Hill, Cindy Bethel, Sue Boonlayangoor, Kathleen G Beavis
The Accelerate Pheno™ system uses automated fluorescence in situ hybridization technology with morphokinetic cellular analysis to provide rapid species identification (ID) and antimicrobial susceptibility testing (AST) results for the most commonly identified organisms in bloodstream infections.Objective was to evaluate the accuracy and workflow of bacterial and yeast ID, and bacterial AST using the Accelerate Pheno™ system in the clinical microbiology laboratory.Consecutive fresh blood cultures received in the laboratory were analyzed by the Accelerate Pheno™ system within 0 - 8 h of growth detection...
November 8, 2017: Journal of Clinical Microbiology
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