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https://www.readbyqxmd.com/read/29672788/neutrophil-extracellular-traps-participate-in-all-different-types-of-thrombotic-and-haemorrhagic-complications-of-coronary-atherosclerosis
#1
Kartika R Pertiwi, Allard C van der Wal, Dara R Pabittei, Claire Mackaaij, Marinus B van Leeuwen, Xiaofei Li, Onno J de Boer
Acute coronary syndromes can be initiated by either atherosclerotic fibrous cap ruptures, superficial plaque erosions or intraplaque haemorrhages (IPHs). Since neutrophil extracellular traps (NETs) display pro-inflammatory and pro-thrombotic properties, we investigated the presence, extent and distribution of neutrophils and NETs in different types of plaque complications in relation to the age of overlying thrombus mass or haemorrhage. Sixty-four paraffin-embedded coronary plaque segments of 30 acute myocardial infarction patients were retrieved from the autopsy archives, which contained 44 complicated plaques (17 IPHs, 9 erosions and 18 ruptures) and 20 intact plaques...
April 19, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29643810/localized-delivery-of-cl-amidine-from-electrospun-polydioxanone-templates-to-regulate-acute-neutrophil-netosis-a-preliminary-evaluation-of-the-pad4-inhibitor-for-tissue-engineering
#2
Allison E Fetz, Indira Neeli, Karyl K Buddington, Robert W Read, Matthew P Smeltzer, Marko Z Radic, Gary L Bowlin
Upon interaction, neutrophils can potentially release neutrophil extracellular traps (NETs) on the surface of an implanted electrospun template, which may be a significant preconditioning event for implantable biomaterials of yet unknown consequences. In this study, we investigated the potential of polydioxanone templates as a delivery vehicle for Cl-amidine, an inhibitor of peptidyl arginase deiminase 4 (PAD4), and if drug elution could attenuate PAD4-mediated NETosis in the vicinity of implanted templates...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/29618953/dna-area-and-netosis-analysis-dana-a-high-throughput-method-to-quantify-neutrophil-extracellular-traps-in-fluorescent-microscope-images
#3
Ryan Rebernick, Lauren Fahmy, Christopher Glover, Mandar Bawadekar, Daeun Shim, Caitlyn L Holmes, Nicole Rademacher, Hemanth Potluri, Christie M Bartels, Miriam A Shelef
Background: Neutrophil extracellular traps (NETs), extracellular structures composed of decondensed chromatin and antimicrobial molecules, are released in a process called NETosis. NETs, which are part of normal host defense, have also been implicated in multiple human diseases. Unfortunately, methods for quantifying NETs have limitations which constrain the study of NETs in disease. Establishing optimal methods for NET quantification holds the potential to further elucidate the role of NETs in normal and pathologic processes...
2018: Biological Procedures Online
https://www.readbyqxmd.com/read/29602707/neutrophil-extracellular-traps-were-released-during-intraoperative-blood-salvage-in-posterior-lumbar-surgery
#4
Li Zheng, Ming Tian, Ye Zhang, Peng Dong, He Yang
The formation of neutrophil extracellular traps (NETs) has been associated with endothelial damage and severe pulmonary dysfunction. The present study aimed to investigate whether this NETosis occurs during intraoperative blood salvage (IBS), and whether the washing procedures before re-transfusion of autologous blood could remove the NET components, including DNA, histones, and myeloperoxidase. The study was performed using blood samples from 20 patients who underwent posterior lumbar surgery at the Beijing Friendship Hospital...
March 14, 2018: Transfusion and Apheresis Science
https://www.readbyqxmd.com/read/29596303/phasor-based-endogenous-nad-p-h-fluorescence-lifetime-imaging-unravels-specific-enzymatic-activity-of-neutrophil-granulocytes-preceding-netosis
#5
Ruth Leben, Lennard Ostendorf, Sofie van Koppen, Asylkhan Rakhymzhan, Anja E Hauser, Helena Radbruch, Raluca A Niesner
Time-correlated single-photon counting combined with multi-photon laser scanning microscopy has proven to be a versatile tool to perform fluorescence lifetime imaging in biological samples and, thus, shed light on cellular functions, both in vitro and in vivo. Here, by means of phasor-analyzed endogenous NAD(P)H (nicotinamide adenine dinucleotide (phosphate)) fluorescence lifetime imaging, we visualize the shift in the cellular metabolism of healthy human neutrophil granulocytes during phagocytosis of Staphylococcus aureus pHrodo™ beads...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29593709/hypertonic-saline-suppresses-nadph-oxidase-dependent-neutrophil-extracellular-trap-formation-and-promotes-apoptosis
#6
Ajantha Nadesalingam, Jacky H K Chen, Armin Farahvash, Meraj A Khan
Tonicity of saline (NaCl) is important in regulating cellular functions and homeostasis. Hypertonic saline is administered to treat many inflammatory diseases, including cystic fibrosis. Excess neutrophil extracellular trap (NET) formation, or NETosis, is associated with many pathological conditions including chronic inflammation. Despite the known therapeutic benefits of hypertonic saline, its underlying mechanisms are not clearly understood. Therefore, we aimed to elucidate the effects of hypertonic saline in modulating NETosis...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29588315/cholesterol-efflux-pathways-suppress-inflammasome-activation-netosis-and-atherogenesis
#7
Marit Westerterp, Panagiotis Fotakis, Mireille Ouimet, Andrea E Bochem, Hanrui Zhang, Matthew M Molusky, Wei Wang, Sandra Abramowicz, Sacha la Bastide-van Gemert, Nan Wang, Carrie L Welch, Muredach P Reilly, Erik S Stroes, Kathryn J Moore, Alan R Tall
Background -The CANTOS trial showed that antagonism of interleukin-1β (IL-1β) reduces coronary heart disease in patients with a previous myocardial infarction and evidence of systemic inflammation, indicating that pathways required for IL-1β secretion increase cardiovascular risk. IL-1β and IL-18 secretion are produced via the NLRP3 inflammasome in myeloid cells in response to cholesterol accumulation, but mechanisms linking NLRP3 inflammasome activation to atherogenesis are unclear. The cholesterol transporters ATP Binding Cassette A1 and G1 (ABCA1/G1) mediate cholesterol efflux to high-density-lipoprotein (HDL) and Abca1/g1 deficiency in myeloid cells leads to cholesterol accumulation...
March 27, 2018: Circulation
https://www.readbyqxmd.com/read/29572545/netosis-complement-and-coagulation-a-triangular-relationship
#8
Cynthia M de Bont, Wilbert C Boelens, Ger J M Pruijn
NETosis is a regulated form of neutrophil cell death that contributes to the host defense against pathogens and was linked to various diseases soon after its first description in 2004. During NETosis, neutrophils release neutrophil extracellular traps (NETs), which can capture and kill bacteria and other pathogens to prevent them from spreading. Although substantial progress has been made in our understanding of NETosis, the precise mechanism underlying NETosis is still a matter of debate. Research continues to elucidate the molecular pathways involved in NETosis...
March 23, 2018: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29572206/roles-of-pad4-and-netosis-in-experimental-atherosclerosis-and-arterial-injury-implications-for-superficial-erosion
#9
Grégory Franck, Thomas L Mawson, Eduardo J Folco, Roberto Molinaro, Victoria Ruvkun, Daniel Engelbertsen, Xin Liu, Yevgenia Tesmenitsky, Eugenia Shvartz, Galina K Sukhova, Jean-Baptiste Michel, Antonino Nicoletti, Andrew H Lichtman, Denisa D Wagner, Kevin J Croce, Peter Libby
<u>Rationale:</u> Neutrophils likely contribute to the thrombotic complications of human atheromata. In particular, neutrophil extracellular traps (NETs) could exacerbate local inflammation and amplify and propagate arterial intimal injury and thrombosis. Peptidyl arginine deiminase 4 (PAD4) participates in NET formation, but understanding of this enzyme's role in atherothrombosis remains scant. <u>Objective:</u> This study tested the hypothesis that PAD4 and NETs influence experimental atherogenesis and in processes implicated in superficial erosion, a form of plaque complication we previously associated with NETs...
March 23, 2018: Circulation Research
https://www.readbyqxmd.com/read/29564336/platelet-microparticles-and-mirna-transfer-in-cancer-progression-many-targets-modes-of-action-and-effects-across-cancer-stages
#10
Sophia Lazar, Lawrence E Goldfinger
Platelet-derived microparticles (PMPs) have long been known to increase in circulation in the presence of cancer, and have been considered to be cancer promoting by multiple mechanisms including shrouding of circulating tumor cells allowing immune evasion, inducing a procoagulant state associated with increased risk for venous thromboembolic events in cancer patients, and supporting metastatic dissemination by establishment of niches for anchorage of circulating tumor cells. These modes of PMP-enhanced progression of late stage cancer are generally based on the adhesive and procoagulant surfaces of PMPs...
2018: Frontiers in Cardiovascular Medicine
https://www.readbyqxmd.com/read/29556228/development-and-application-of-high-content-biological-screening-for-modulators-of-net-production
#11
Ilaria J Chicca, Michael R Milward, Iain Leslie C Chapple, Gareth Griffiths, Rod Benson, Thomas Dietrich, Paul R Cooper
Neutrophil extracellular traps (NETs) are DNA-based antimicrobial web-like structures whose release is predominantly mediated by reactive oxygen species (ROS); their purpose is to combat infections. However, unbalanced NET production and clearance is involved in tissue injury, circulation of auto-antibodies and development of several chronic diseases. Currently, there is lack of agreement regarding the high-throughput methods available for NET investigation. This study, therefore, aimed to develop and optimize a high-content analysis (HCA) approach, which can be applied for the assay of NET production and for the screening of compounds involved in the modulation of NET release...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29550813/a-role-for-receptor-interacting-protein-kinase-1-in-neutrophil-extracellular-trap-formation-in-patients-with-systemic-lupus-erythematosus-a-preliminary-study
#12
Ruru Guo, Yang Tu, Shaowei Xie, Xue Song Liu, Yang Song, Suli Wang, Xiaoxiang Chen, Liangjing Lu
BACKGROUND/AIMS: Neutrophil extracellular traps (NETs) are known to play an important role in systemic lupus erythematosus (SLE) by triggering innate and adaptive immune responses. The molecular mechanisms responsible for their formation in SLE are still unclear. In this study, we aim to characterize the role of the receptor-interacting protein kinase-1 (RIPK1), a homologous serine/threonine kinase previously implicated in the regulation of necroptosis and tissue injury, in decreasing neutrophil death and formation of NETs, and to investigate the clinical implications of RIPK1 in SLE...
March 13, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29546579/the-antidiabetic-drug-metformin-blunts-netosis-in-vitro-and-reduces-circulating-netosis-biomarkers-in-vivo
#13
Lisa Menegazzo, Valentina Scattolini, Roberta Cappellari, Benedetta Maria Bonora, Mattia Albiero, Mario Bortolozzi, Filippo Romanato, Giulio Ceolotto, Saula Vigili de Kreutzeberg, Angelo Avogaro, Gian Paolo Fadini
AIMS: Diabetes is associated with an excess release of neutrophil extracellular traps (NETs) and an enhanced NETosis, a neutrophil cell death programme instrumental to anti-microbial defences, but also involved in tissue damage. We herein investigated whether the antidiabetic drug metformin protects against NETosis. METHODS: We measured NET components in the plasma of patients with pre-diabetes who were randomized to receive metformin or placebo for 2 months. To control for the effect on glucose, we also measured NET components in the plasma of patients with type 2 diabetes before and after treatment with insulin or dapagliflozin...
March 15, 2018: Acta Diabetologica
https://www.readbyqxmd.com/read/29533770/neutrophil-extracellular-traps-the-biology-of-chromatin-externalization
#14
REVIEW
Gabriel Sollberger, Dorothea Ogmore Tilley, Arturo Zychlinsky
Neutrophils are essential to the homeostatic mission of safeguarding host tissues, responding rapidly and diversely to breaches of the host's barriers to infection, and returning tissues to a sterile state. In response to specific stimuli, neutrophils extrude modified chromatin structures decorated with specific cytoplasmic and granular proteins called neutrophil extracellular traps (NETs). Several pathways lead to this unique form of cell death (NETosis). Extracellular chromatin may have evolved to defend eukaryotic organisms against infection, and its release has at least three functions: trapping and killing of microbes, amplifying immune responses, and inducing coagulation...
March 12, 2018: Developmental Cell
https://www.readbyqxmd.com/read/29519921/markers-of-neutrophil-extracellular-traps-predict-adverse-outcome-in-community-acquired-pneumonia-secondary-analysis-of-a-randomised-controlled-trial
#15
Fahim Ebrahimi, Stavros Giaglis, Sinuhe Hahn, Claudine A Blum, Christine Baumgartner, Alexander Kutz, Shane Vontelin van Breda, Beat Mueller, Philipp Schuetz, Mirjam Christ-Crain, Paul Hasler
Neutrophil extracellular traps (NETs) are a hallmark of the immune response in inflammatory diseases. However, the role of NETs in community-acquired pneumonia is unknown. This study aims to characterise the impact of NETs on clinical outcomes in pneumonia.This is a secondary analysis of a randomized-controlled, multicentre trial. Patients with community-acquired pneumonia were randomly assigned to either prednisone 50 mg or placebo for 7 days. The primary endpoint was time to clinical stability; main secondary endpoints were length of hospital stay and mortality...
March 8, 2018: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/29487850/regulating-netosis-increasing-ph-promotes-nadph-oxidase-dependent-netosis
#16
Meraj A Khan, Lijy M Philip, Guillaume Cheung, Shawn Vadakepeedika, Hartmut Grasemann, Neil Sweezey, Nades Palaniyar
Neutrophils migrating from the blood (pH 7.35-7.45) into the surrounding tissues encounter changes in extracellular pH (pHe ) conditions. Upon activation of NADPH oxidase 2 (Nox), neutrophils generate large amounts of H+ ions reducing the intracellular pH (pHi ). Nevertheless, how extracellular pH regulates neutrophil extracellular trap (NET) formation (NETosis) is not clearly established. We hypothesized that increasing pH increases Nox-mediated production of reactive oxygen species (ROS) and neutrophil protease activity, stimulating NETosis...
2018: Frontiers in Medicine
https://www.readbyqxmd.com/read/29472233/novel-role-of-il-interleukin-1%C3%AE-in-neutrophil-extracellular-trap-formation-and-abdominal-aortic-aneurysms
#17
Akshaya K Meher, Michael Spinosa, John P Davis, Nicolas Pope, Victor E Laubach, Gang Su, Vlad Serbulea, Norbert Leitinger, Gorav Ailawadi, Gilbert R Upchurch
OBJECTIVE: Neutrophils promote experimental abdominal aortic aneurysm (AAA) formation via a mechanism that is independent from MMPs (matrix metalloproteinases). Recently, we reported a dominant role of IL (interleukin)-1β in the formation of murine experimental AAAs. Here, the hypothesis that IL-1β-induced neutrophil extracellular trap formation (NETosis) promotes AAA was tested. APPROACH AND RESULTS: NETs were identified through colocalized staining of neutrophil, Cit-H3 (citrullinated histone H3), and DNA, using immunohistochemistry...
February 22, 2018: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/29462936/new-insights-into-the-immune-molecular-regulation-of-the-pathogenesis-of-acute-respiratory-distress-syndrome
#18
REVIEW
Chin-Yao Yang, Chien-Sheng Chen, Giou-Teng Yiang, Yeung-Leung Cheng, Su-Boon Yong, Meng-Yu Wu, Chia-Jung Li
Acute respiratory distress syndrome is an inflammatory disease characterized by dysfunction of pulmonary epithelial and capillary endothelial cells, infiltration of alveolar macrophages and neutrophils, cell apoptosis, necroptosis, NETosis, and fibrosis. Inflammatory responses have key effects on every phase of acute respiratory distress syndrome. The severe inflammatory cascades impaired the regulation of vascular endothelial barrier and vascular permeability. Therefore, understanding the relationship between the molecular regulation of immune cells and the pulmonary microenvironment is critical for disease management...
February 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29459871/-staphylococcal-protein-a-is-a-key-factor-in-neutrophil-extracellular-traps-formation
#19
Tamara Hoppenbrouwers, Andi R Sultan, Tsion E Abraham, Nicole A Lemmens-den Toom, Silvie Hansenová Maňásková, Wiggert A van Cappellen, Adriaan B Houtsmuller, Willem J B van Wamel, Moniek P M de Maat, Johan W van Neck
Staphylococcus aureus are strong inducers of neutrophil extracellular traps (NETs), a defense mechanism of neutrophils against pathogens. Our aim was to explore the role of Protein A in S. aureus -induced NETosis. We determined the Protein A production of four different S. aureus strains and found a direct relationship between the degree of NETosis induction and Protein A production: strains producing higher concentrations of Protein A evoke significantly more NETs. A S. aureus strain in which Protein A as well as a second binding protein for immunoglobulins ( Sbi ) have been knocked-out (Δ SpA Δ Sbi ) induced significantly less NETosis than the wild-type strain...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29447842/neutrophils-drive-type-i-interferon-production-and-autoantibodies-in-patients-with-wiskott-aldrich-syndrome
#20
Karla E Cervantes-Luevano, Nicoletta Caronni, Maria C Castiello, Elena Fontana, Giulia M Piperno, Asma Naseem, Paolo Uva, Marita Bosticardo, Genni E Marcovecchio, Luigi D Notarangelo, Maria P Cicalese, Alessandro Aiuti, Anna Villa, Federica Benvenuti
BACKGROUND: Wiskott-Aldrich syndrome (WAS) is a rare primary immunodeficiency caused by mutations in Wiskott-Aldrich syndrome protein (WASp), a key regulator of cytoskeletal dynamics in hematopoietic cells. A high proportion of patients experience autoimmunity caused by a breakdown in T- and B-cell tolerance. Moreover, excessive production of type I interferon (IFN-I) by plasmacytoid dendritic cells (pDCs) contributes to autoimmune signs; however, the factors that trigger excessive innate activation have not been defined...
February 13, 2018: Journal of Allergy and Clinical Immunology
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