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https://www.readbyqxmd.com/read/28738585/ordered-mesoporous-silica-film-as-a-novel-fiber-coating-for-solid-phase-microextraction
#1
Hui Jiang, Jiansheng Li, Xingru Hu, Jinyou Shen, Xiuyun Sun, Weiqing Han, Lianjun Wang
Ordered mesoporous silica (OMS) film supported on anodized titanium wire was fabricated as a new solid phase microextraction (SPME) fiber for the extraction of chlorophenols (CPs) from aqueous media followed by the determination with gas chromatography flame ionization detection (GC-FID). The supported OMS film was prepared via a solvent evaporation-induced self-assembly approach (EISA) combined with dip-coating technology. Commonly used fragile fused silica fiber was replaced by anodized titanium wire. The coating materials were characterized by N2 adsorption-desorption, Fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and thermogravimetric (TG) analysis...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738543/phenolic-compounds-of-triplaris-gardneriana-can-protect-cells-against-oxidative-stress-and-restore-oxidative-balance
#2
Thiago Silva de Almeida, José Joaquim Lopes Neto, Nathanna Mateus de Sousa, Igor Parra Pessoa, Leonardo Rogério Vieira, Jackeline Lima de Medeiros, Aline Augusti Boligon, Astrid R M Hamers, Davi Felipe Farias, Ad Peijnenburg, Ana Fontenele Urano Carvalho
This work aimed to add value to an underexploited plant species from Brazil, Triplaris gardneriana. To that, the phenolic compounds profile of its seed ethanolic extract and fractions was examined by HPLC and the antioxidant capacity assessed using chemical assays as well as in vitro cell imaging. Twelve compounds were quantified and classified as either phenolic acids or flavonoids. The fractionation process did not generate fractions with different compositions except for chloroformic fraction, which showed only 6 out of 12 standard compounds used...
July 20, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28738229/a-facile-one-step-method-for-cell-lysis-and-dna-extraction-of-waterborne-pathogens-using-a-microchip
#3
Vivek Kamat, Sulaxna Pandey, Kishore Paknikar, Dhananjay Bodas
Globally, waterborne organisms are the primary causative agents for the transmission of various forms of diarrheal diseases. For accurate diagnosis, molecular tools have gained considerable attention in the recent past. Molecular tools require DNA as the starting material for diagnosis, and hence, a prerequisite is the quality and integrity of DNA. To obtain high quality DNA rapidly, we have fabricated a microchip in poly(dimethyl siloxane) (PDMS) by soft lithography process. The microchip facilitated in-flow coating of chitosan on the magnetic nanoparticles, which under external mechanical vibration caused cell lysis and released DNA in the supernatant...
July 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28737267/appearance-and-characterization-of-fruit-image-textures-for-quality-sorting-using-wavelet-transform-and-genetic-algorithms
#4
Suchitra Khoje
Images of four qualities of Mangoes and Guavas are evaluated for colour and textural features to characterize and classify them, and to model the fruit appearance grading. The paper discusses three approaches to identify most discriminating texture features of both the fruits. In the first approach, fruit's colour and texture features are selected using Mahalanobis distance. A total of 20 colour features and 40 textural features are extracted for analysis. Using Mahalanobis distance and feature inter-correlation analyses, one best colour features [mean of a* (L*a*b* colour space) and two textural features [energy a*, contrast of H*]are selected as features for guava while two best colour features [R std, H std] and one textural features [energy b*]are selected as features for Mangoes with the highest discriminate power...
July 24, 2017: Journal of Texture Studies
https://www.readbyqxmd.com/read/28736704/visualization-of-photoacoustic-images-in-a-limited-view-measuring-system-using-eigenvalues-of-a-photoacoustic-transmission-matrix
#5
Hiroshi Abe, Tsuyoshi Shiina
Photoacoustic imaging is a unique imaging method that involves extracting information from points at different depths, an advantage of ultrasound imaging, while maintaining functional information, a key feature of conventional photo imaging. This makes it easy to add functional images to ultrasound images by adding a laser pulse source to the conventional ultrasound imaging device and detecting a photo-ultrasound signal via a conventional ultrasound probe. One challenge when using normal one-dimensional (1D) probes and generating photoacoustic images is the limited-view problem, in which artefacts are observed due to the positions of the ultrasound transducers...
December 2017: Photoacoustics
https://www.readbyqxmd.com/read/28736674/fluorescein-as-a-topical-fluorescent-contrast-agent-for-quantitative-microendoscopic-inspection-of-colorectal-epithelium
#6
Sandra P Prieto, Keith K Lai, Jonathan A Laryea, Jason S Mizell, William C Mustain, Timothy J Muldoon
Fiber bundle microendoscopic imaging of colorectal tissue has shown promising results, for both qualitative and quantitative analysis. A quantitative image quality control and image feature extraction algorithm was previously designed for quantitative image feature analysis of proflavine-stained ex vivo colorectal tissue. We investigated fluorescein as an alternative topical stain. Images of ex vivo porcine, caprine, and human colorectal tissue were used to compare microendoscopic images of tissue topically stained with fluorescein and proflavine solutions...
April 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28736471/comparison-of-multi-fiber-reproducibility-of-pas-mri-and-q-ball-with-empirical-multiple-b-value-hardi
#7
Vishwesh Nath, Kurt G Schilling, Justin A Blaber, Zhaohua Ding, Adam W Anderson, Bennett A Landman
Crossing fibers are prevalent in human brains and a subject of intense interest for neuroscience. Diffusion tensor imaging (DTI) can resolve tissue orientation but is blind to crossing fibers. Many advanced diffusion-weighted magnetic resolution imaging (MRI) approaches have been presented to extract crossing-fibers from high angular resolution diffusion imaging (HARDI), but the relative sensitivity and specificity of approaches remains unclear. Here, we examine two leading approaches (PAS and q-ball) in the context of a large-scale, single subject reproducibility study...
February 11, 2017: Proceedings of SPIE
https://www.readbyqxmd.com/read/28736352/the-effect-of-biocomposite-screws-on-bone-regeneration-in-a-rat-osteoporosis-model
#8
Je Hoon Jeong, Eun-Sun Jin, Ji Yeon Kim, Bora Lee, JoongKee Min, Sang Ryong Jeon, Minsu Lee, Kyoung Hyo Choi
INTRODUCTION: The aim of this study was to evaluate the efficacy of biocomposite screws used in ovariectomy (OVX) induced osteoporotic rats. METHODS: Twenty-four female Wistar rats (250-300g, 12 weeks old) were divided into 4 groups; Sham group (control), OVX-induced osteoporosis group (OVX), OVX and biodegradable (PLGA (poly-lactic-co-glycolic acid) without ß-TCP (tricalcium phosphate) screw insertion group (OVX/BSR), OVX and biocomposite (PLGA with ß-TCP) screw insertion group (OVX/CSR)...
July 20, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/28735789/xp-shaper-a-novel-adaptive-core-rotary-instrument-micro-computed-tomographic-analysis-of-its-shaping-abilities
#9
Adham A Azim, Lucila Piasecki, Ulisses Xavier da Silva Neto, Alessandra Timponi Goes Cruz, Katharina A Azim
INTRODUCTION: The aim of this study was to investigate the shaping abilities of the XP Shaper (FKG, La Chaux-de-Fonds, Switzerland) and compare the findings with Vortex Blue (Dentsply Tulsa Dental Specialties, Tulsa, OK) using micro-computed tomographic imaging. METHODS: Twenty matched, extracted, mandibular, central incisors with a single, oval canal were scanned preoperatively at 25-μm resolution and postoperatively after instrumentation with either Vortex Blue in a crown-down manner up to size 30...
July 20, 2017: Journal of Endodontics
https://www.readbyqxmd.com/read/28734961/radix-puerariae-modulates-glutamatergic-synaptic-architecture-and-potentiates-functional-synaptic-plasticity-in-primary-hippocampal-neurons
#10
Mohammad Maqueshudul Haque Bhuiyan, Md Nazmul Haque, Md Mohibbullah, Yung Kyu Kim, Il Soo Moon
ETHNOPHARMACOLOGICAL RELEVANCE: Neurologic disorders are frequently characterized by synaptic pathology, including abnormal density and morphology of dendritic spines, synapse loss, and aberrant synaptic signaling and plasticity. Therefore, to promote and/or protect synapses by the use of natural molecules capable of modulating neurodevelopmental events, such as, spinogenesis and synaptic plasticity, could offer a preventive and curative strategy for nervous disorders associated with synaptic pathology...
July 19, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28734530/joint-optic-disc-and-cup-boundary-extraction-from-monocular-fundus-images
#11
Arunava Chakravarty, Jayanthi Sivaswamy
BACKGROUND AND OBJECTIVE: Accurate segmentation of optic disc and cup from monocular color fundus images plays a significant role in the screening and diagnosis of glaucoma. Though optic cup is characterized by the drop in depth from the disc boundary, most existing methods segment the two structures separately and rely only on color and vessel kink based cues due to the lack of explicit depth information in color fundus images. METHODS: We propose a novel boundary-based Conditional Random Field formulation that extracts both the optic disc and cup boundaries in a single optimization step...
August 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28734527/a-novel-method-to-identify-and-grade-dna-damage-on-comet-images
#12
Muhammed Kamil Turan, Eftal Sehirli
BACKGROUND AND OBJECTIVE: In recent years, development of software programs in medicine field has proceeded in a rapid manner. Comet assay is one of the research methods in medicine field that displays whether DNA has damage. In this study, it is aimed to share experience of dynamic time warping method and decision tree to decide whether DNA has damage and grade DNA damage by means of the software program. METHODS: The application analyzes manually extracted RGB single comet images whose centers are manually marked...
August 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28734194/high-yield-one-pot-production-of-18-f-fch-via-a-modified-tracerlab-fxfn-module
#13
Ya-Yao Huang, Chia-Ling Tsai, Hsiang-Ping Wen, Kai-Yuan Tzen, Ruoh-Fen Yen, Chyng-Yann Shiue
INTRODUCTION: [(18)F]Fluoromethylcholine ([(18)F]FCH) is a potent tumors imaging agent. In order to fulfill the demand of pre-clinical and clinical studies, we have developed an automated high yield one-pot synthesis of this potent tumors imaging agent. METHODS: [(18)F]FCH was synthesized using a modified TRACERlab FxFN module. Briefly, dibromomethane (10% in CH3CN) was fluorinated with K[(18)F]/K 2.2.2 in a glassy carbon reaction vessel at 120°C for about 5min to generate [(18)F]fluorobromomethane ([(18)F]FBM)...
July 15, 2017: Applied Radiation and Isotopes
https://www.readbyqxmd.com/read/28734161/evaluation-of-the-effect-of-extraction-solvent-and-organ-selection-on-the-chemical-profile-of-astragalus-spinosus-using-hptlc-multivariate-image-analysis
#14
Eman Shawky, Dina A Selim
The evaluation of extraction protocols for untargeted and targeted metabolomics was implemented for root and aerial organs of Astragalus spinosus in this work. The efficiency and complementarity of commonly used extraction solvents, namely petroleum ether, methylene chloride, ethyl acetate and n-butanol were considered for method evaluation using chemometric techniques in conjunction with new, simple, and fast high performance thin layer chromatography (HPTLC) method for fingerprint analysis by extracting information from a digitalized HPTLC plate using ImageJ software...
July 15, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/28733815/structure-of-isolated-z-disks-from-honeybee-flight-muscle
#15
Mara Rusu, Zhongjun Hu, Kenneth A Taylor, John Trinick
The Z-disk is a complex structure comprising some 40 proteins that are involved in the transmission of force developed during muscle contraction and in important signalling pathways that govern muscle homeostasis. In the Z-disk the ends of antiparallel thin filaments from adjacent sarcomeres are crosslinked by α-actinin. The structure of the Z-disk lattice varies greatly throughout the animal kingdom. In vertebrates the thin filaments form a tetragonal lattice, whereas invertebrate flight muscle has a hexagonal lattice...
July 21, 2017: Journal of Muscle Research and Cell Motility
https://www.readbyqxmd.com/read/28733406/genetic-interaction-score-s-score-calculation-clustering-and-visualization-of-genetic-interaction-profiles-for-yeast
#16
Assen Roguev, Colm J Ryan, Jiewei Xu, Isabelle Colson, Edgar Hartsuiker, Nevan Krogan
This protocol describes computational analysis of genetic interaction screens, ranging from data capture (plate imaging) to downstream analyses. Plate imaging approaches using both digital camera and office flatbed scanners are included, along with a protocol for the extraction of colony size measurements from the resulting images. A commonly used genetic interaction scoring method, calculation of the S-score, is discussed. These methods require minimal computer skills, but some familiarity with MATLAB and Linux/Unix is a plus...
July 21, 2017: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/28732991/in-situ-observation-of-ultrasonic-cavitation-induced-fragmentation-of-the-primary-crystals-formed-in-al-alloys
#17
Feng Wang, Iakovos Tzanakis, Dmitry Eskin, Jiawei Mi, Thomas Connolley
The cavitation-induced fragmentation of primary crystals formed in Al alloys were investigated for the first time by high-speed imaging using a novel experimental approach. Three representative primary crystal types, Al3Ti, Si and Al3V with different morphologies and mechanical properties were first extracted by deep etching of the corresponding Al alloys and then subjected to ultrasonic cavitation processing in distilled water. The dynamic interaction between the cavitation bubbles and primary crystals was imaged in situ and in real time...
November 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28732775/statistical-analysis-of-the-inter-individual-variations-of-the-bone-shape-volume-fraction-and-fabric-and-their-correlations-in-the-proximal-femur
#18
Elham Taghizadeh, Vimal Chandran, Mauricio Reyes, Philippe Zysset, Philippe Büchler
Including structural information of trabecular bone improves the prediction of bone strength and fracture risk. However, this information is available in clinical CT scans, only for peripheral bones. We hypothesized that a correlation exists between the shape of the bone, its volume fraction (BV/TV) and fabric, which could be characterized using statistical modeling. High-resolution peripheral computed tomography (HR-pQCT) images of 73 proximal femurs were used to build a combined statistical model of shape, BV/TV and fabric...
July 18, 2017: Bone
https://www.readbyqxmd.com/read/28732648/a-systematic-review-of-women-s-satisfaction-and-regret-following-risk-reducing-mastectomy
#19
REVIEW
Lucy Braude, Laura Kirsten, Jemma Gilchrist, Ilona Juraskova
OBJECTIVE: A systematic review of quantitative and qualitative studies, to describe patient satisfaction and regret associated with risk-reducing mastectomies (RRM), and the patient-reported factors associated with these among women at high risk of developing breast cancer. METHODS: Studies were identified using Medline, CINAHL, Embase and PsycInfo databases (1995-2016). Data were extracted and crosschecked for accuracy. Article quality was assessed using standardised criteria...
July 1, 2017: Patient Education and Counseling
https://www.readbyqxmd.com/read/28732616/stromal-remodeling-and-lenticule-thickness-accuracy-in-small-incision-lenticule-extraction-one-year-results
#20
Nikolaus Luft, Siegfried G Priglinger, Michael H Ring, Wolfgang J Mayer, Anna S Mursch-Edlmayr, Thomas C Kreutzer, Matthias Bolz, Martin Dirisamer
PURPOSE: To prospectively characterize the stromal thickness changes during the first year after myopic small-incision lenticule extraction using spectral-domain optical coherence tomography (SD-OCT). SETTING: Department of Ophthalmology, Kepler University Hospital, Linz, Austria. DESIGN: Prospective case series. METHODS: This study evaluated eyes that had small-incision lenticule extraction to treat myopia or myopic astigmatism...
June 2017: Journal of Cataract and Refractive Surgery
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