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https://www.readbyqxmd.com/read/28326637/purinergic-receptors-p2rx4-and-p2rx7-in-familial-multiple-sclerosis
#1
A Dessa Sadovnick, Ben J Gu, Anthony L Traboulsee, Cecily Q Bernales, Mary Encarnacion, Irene M Yee, Maria G Criscuoli, Xin Huang, Amber Ou, Carol J Milligan, Steven Petrou, James S Wiley, Carles Vilariño-Güell
Genetic variants in the purinergic receptors P2RX4 and P2RX7 have been shown to affect susceptibility to multiple sclerosis (MS). In this study we set out to evaluate whether rare coding variants of major effect could also be identified in these purinergic receptors. Sequencing analysis of P2RX4 and P2RX7 in 193 MS patients and 100 controls led to the identification of a rare three variant haplotype (P2RX7 rs140915863:C>T (p.T205M), P2RX7 rs201921967:A>G (p.N361S) and P2RX4 rs765866317:G>A (p.G135S)) segregating with disease in a multi-incident family with six family members diagnosed with MS (LOD = 3...
March 21, 2017: Human Mutation
https://www.readbyqxmd.com/read/28292763/oxygen-delivery-and-the-restoration-of-the-muscle-energetic-balance-following-exercise-implications-for-delayed-muscle-recovery-in-patients-with-copd
#2
Gwenael Layec, Corey R Hart, Joel D Trinity, Oh-Sung Kwon, Matthew J Rossman, Ryan M Broxterman, Yann Le Fur, Eun-Kee Jeong, Russell S Richardson
Patients with chronic obstructive pulmonary disease (COPD) experience a delayed recovery from skeletal muscle fatigue following exhaustive exercise that likely contributes to their progressive loss of mobility. As this phenomenon is not well understood, this study sought to examine post-exercise peripheral oxygen (O2) transport and muscle metabolism dynamics in patients with COPD, two important determinants of muscle recovery. Twenty four subjects, 12 non-hypoxemic patients with COPD and 12 healthy subjects with a sedentary lifestyle, performed dynamic plantar flexion exercise at 40% of maximal work rate (WRmax) with phosphorus magnetic resonance spectroscopy ((31)P-MRS), near-infrared spectroscopy (NIRS), and vascular Doppler ultrasound assessments...
March 14, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28278051/bcl-2-delays-cell-cycle-through-mitochondrial-atp-and-ros
#3
Xing Du, Xufeng Fu, Kun Yao, Zhenwei Lan, Hui Xu, Qinghua Cui, Elizabeth Yang
Bcl-2 inhibits cell proliferation by delaying G0/G1 to S phase entry. We tested the hypothesis that Bcl-2 regulates S phase entry through mitochondrial pathways. Existing evidence indicates mitochondrial adenosine tri-phosphate (ATP) and reactive oxygen species (ROS) are important signals in cell survival and cell death, however, the molecular details of how these 2 processes are linked remain unknown. In this study, 2 cell lines stably expressing Bcl-2, 3T3Bcl-2 and C3HBcl-2, and vector-alone PB controls were arrested in G0/G1 phase by serum starvation and contact inhibition, and ATP and ROS were measured during re-stimulation of cell cycle entry...
February 22, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28259604/bacterial-contamination-of-ct-equipment-use-of-atp-detection-and-culture-results-to-target-quality-improvement
#4
John Childress, Debborah Burch, Cheryl Kucharski, Carol Young, Ella A Kazerooni, Matthew S Davenport
RATIONALE AND OBJECTIVE: This study aimed to evaluate the use of an adenosine triphosphate (ATP) monitoring system to minimize surface contamination on inpatient computed tomography (CT) scanners. METHODS: The bore, table, and wrap of two quaternary care inpatient CT scanners (load/scanner: ~ 30-40 CT examinations/day) were assayed with bacterial cultures and an ATP detection system during six prospective iterative plan-do-check-act improvement cycles from January 6, 2016 to October 12, 2016...
March 1, 2017: Academic Radiology
https://www.readbyqxmd.com/read/28244131/phosphodiester-content-measured-in-human-liver-by-in-vivo-31-p-mr-spectroscopy-at-7-tesla
#5
Lucian A B Purvis, William T Clarke, Ladislav Valkovič, Christina Levick, Michael Pavlides, Eleanor Barnes, Jeremy F Cobbold, Matthew D Robson, Christopher T Rodgers
PURPOSE: Phosphorus ((31) P) metabolites are emerging liver disease biomarkers. Of particular interest are phosphomonoester and phosphodiester (PDE) "peaks" that comprise multiple overlapping resonances in (31) P spectra. This study investigates the effect of improved spectral resolution at 7 Tesla (T) on quantifying hepatic metabolites in cirrhosis. METHODS: Five volunteers were scanned to determine metabolite T1 s. Ten volunteers and 11 patients with liver cirrhosis were scanned at 7T...
February 28, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28219591/correlation-between-survival-interval-and-cd4-t-cell-intracellular-atp-levels-in-liver-transplant-recipients
#6
W Qu, Z-J Zhu, L-Y Sun, L Wei, Y Liu, Z-G Zeng
OBJECTIVE: The objectives of this study were to analyze the potential correlation between post-liver transplantation survival interval and CD4(+) T-cell intracellular ATP (iATP) levels, and to describe the distribution of CD4(+) T-cell iATP levels in liver transplant recipients. METHODS: This was a retrospective analysis of clinical data of 273 patients who underwent liver transplantation from July 2010 to October 2012 in our center and achieved long-term stable survival...
March 2017: Transplantation Proceedings
https://www.readbyqxmd.com/read/28213290/zd0947-a-sulphonylurea-receptor-modulator-detects-functional-sulphonylurea-receptor-subunits-in-murine-vascular-smooth-muscle-atp-sensitive-k-channels
#7
Tadashi Yamamoto, Kohei Takahara, Keiichiro Uchida, Noriyoshi Teramoto
In order to identify functional sulphonylurea receptor (SUR.x) subunits of native ATP-sensitive K(+) channels (KATP channels) in mouse portal vein, the effects of ZD0947, a SUR.x modulator, were investigated on spontaneous portal vein contractions, macroscopic membrane currents and unitary currents recorded (using patch-clamp techniques) in freshly dispersed mouse portal vein myocytes. Spontaneous contractions in mouse portal vein were reversibly reduced by ZD0947 in a concentration-dependent manner (Ki =293nM)...
April 5, 2017: European Journal of Pharmacology
https://www.readbyqxmd.com/read/28209743/skeletal-muscle-bioenergetics-during-all-out-exercise-mechanistic-insight-into-the-oxygen-uptake-slow-component-and-neuromuscular-fatigue
#8
Ryan M Broxterman, Gwenael Layec, Thomas J Hureau, Markus Amann, Russell S Richardson
Although commonly used, the physiological mechanisms underlying all-out exercise performance are still unclear and an in-depth assessment of skeletal muscle bioenergetics is lacking. Therefore, phosphorous magnetic resonance spectroscopy ((31)P-MRS) was utilized to assess skeletal muscle bioenergetics during a 5-min all-out intermittent isometric knee-extensor protocol in 8 healthy men. The metabolic perturbation, adenosine triphosphate (ATP) synthesis rates, ATP cost of contraction, and mitochondrial capacity were determined from intramuscular concentrations of phosphocreatine (PCr), inorganic phosphate (Pi), diprotonated phosphate (H2PO4⁻ ), and pH...
February 16, 2017: Journal of Applied Physiology
https://www.readbyqxmd.com/read/28202574/action-potentials-and-ion-conductances-in-wild-type-and-calhm1-knockout-type-ii-taste-cells
#9
Zhongming Ma, Wint Thu Saung, J Kevin Foskett
Taste bud type II cells fire action potentials in response to tastants, triggering non-vesicular ATP release to gustatory neurons via voltage-gated CALHM1-associated ion channels. Whereas CALHM1 regulates mouse cortical neuron excitability, its roles in regulating type II cell excitability are unknown. Here, we compared membrane conductances and action potentials in single identified TRPM5-GFP-expressing circumvallate papillae type II cells acutely isolated from wild-type (WT) and Calhm1-knockout (KO) mice...
February 15, 2017: Journal of Neurophysiology
https://www.readbyqxmd.com/read/28199326/a-mathematical-model-of-calcium-dynamics-in-hsy-cells
#10
Jung Min Han, Akihiko Tanimura, Vivien Kirk, James Sneyd
Saliva is an essential part of activities such as speaking, masticating and swallowing. Enzymes in salivary fluid protect teeth and gums from infectious diseases, and also initiate the digestion process. Intracellular calcium (Ca2+) plays a critical role in saliva secretion and regulation. Experimental measurements of Ca2+ and inositol trisphosphate (IP3) concentrations in HSY cells, a human salivary duct cell line, show that when the cells are stimulated with adenosine triphosphate (ATP) or carbachol (CCh), they exhibit coupled oscillations with Ca2+ spike peaks preceding IP3 spike peaks...
February 2017: PLoS Computational Biology
https://www.readbyqxmd.com/read/28196853/impaired-cardiac-and-skeletal-muscle-bioenergetics-in-children-adolescents-and-young-adults-with-barth-syndrome
#11
Adil Bashir, Kathryn L Bohnert, Dominic N Reeds, Linda R Peterson, Adam J Bittel, Lisa de Las Fuentes, Christina A Pacak, Barry J Byrne, W Todd Cade
Barth syndrome (BTHS) is an X-linked condition characterized by altered cardiolipin metabolism and cardioskeletal myopathy. We sought to compare cardiac and skeletal muscle bioenergetics in children, adolescents, and young adults with BTHS and unaffected controls and examine their relationships with cardiac function and exercise capacity. Children/adolescents and young adults with BTHS (n = 20) and children/adolescent and young adult control participants (n = 23, total n = 43) underwent (31)P magnetic resonance spectroscopy ((31)P-MRS) of the lower extremity (calf) and heart for estimation of skeletal muscle and cardiac bioenergetics...
February 2017: Physiological Reports
https://www.readbyqxmd.com/read/28196418/automated-algorithm-for-detection-of-transient-adenosine-release
#12
Ryan P Borman, Ying Wang, Michael D Nguyen, Mallikarjunarao Ganesana, Scott T Lee, B Jill Venton
Spontaneous adenosine release events have been discovered in the brain that last only a few seconds. The identification of these adenosine events from fast-scan cyclic voltammetry (FSCV) data is difficult due to the random nature of adenosine release. In this study, we develop an algorithm that automatically identifies and characterizes adenosine transient features, including event time, concentration, and duration. Automating the data analysis reduces analysis time from 10 to 18 h to about 40 min per experiment...
February 15, 2017: ACS Chemical Neuroscience
https://www.readbyqxmd.com/read/28192180/deletion-of-cd73-in-mice-leads-to-aortic-valve-dysfunction
#13
P Zukowska, B Kutryb-Zajac, A Jasztal, M Toczek, M Zabielska, T Borkowski, Z Khalpey, R T Smolenski, E M Slominska
Aortic stenosis is known to involve inflammation and thrombosis. Changes in activity of extracellular enzyme - ecto-5'-nucleotidase (referred also as CD73) can alter inflammatory and thrombotic responses. This study aimed to evaluate the effect of CD73 deletion in mice on development of aortic valve dysfunction and to compare it to the effect of high-fat diet. Four groups of mice (normal-diet Wild Type (WT), high-fat diet WT, normal diet CD73-/-, high-fat diet CD73-/-) were maintained for 15weeks followed by echocardiographic analysis of aortic valve function, measurement of aortic surface activities of nucleotide catabolism enzymes as well as alkaline phosphatase activity, mineral composition and histology of aortic valve leaflets...
February 10, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28159278/the-role-of-microorganisms-in-the-degradation-of-adenosine-triphosphate-atp-in-chill-stored-common-carp-cyprinus-carpio-fillets
#14
Dapeng Li, Longteng Zhang, Sijia Song, Zhiying Wang, Chunli Kong, Yongkang Luo
Biochemical and microbial changes after harvest strongly affect the final quality and shelf life of fish and fish products. In this study, the role of microbes in the degradation of adenosine triphosphate (ATP), and the origin of adenosine monophosphate deaminase (AMPD) and acid phosphatase (ACP) in common carp fillets during different stages of chilled storage (at 4°C) were investigated. The content of ATP, ADP, AMP, IMP, HxR, and Hx, the activity of AMPD and ACP, and the total count of viable, Aeromonas, Pseudomonas, H2S-producing bacteria, and lactic acid bacteria were examined...
June 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28128376/a-highly-reproducible-and-sensitive-fiber-sers-probe-fabricated-by-direct-synthesis-of-closely-packed-agnps-on-the-silanized-fiber-taper
#15
Jie Cao, Di Zhao, Qinghe Mao
A surface-enhanced Raman scattering (SERS) tapered fiber probe has been developed by using a chemically-etched tapered fiber tip and silanization of the surface of the fiber taper, followed by the hydrothermal growth of silver nanoparticles (AgNPs) on the silanized fiber taper. 4-Aminothiophenol (4-ATP) was selected as the target analyte to study the SERS responses of the prepared fiber SERS probe in an optrode remote detection mode. The experimental results show that the prepared fiber probe exhibited the ability to detect the 4-ATP molecule at a concentration as low as 10(-9) M and good reproducibility with the relative standard deviation (RSD) values being less than 9...
January 27, 2017: Analyst
https://www.readbyqxmd.com/read/28127795/weighted-averaging-in-spectroscopic-studies-improves-statistical-power
#16
Jack J Miller, Lowri Cochlin, Kieran Clarke, Damian J Tyler
PURPOSE: In vivo MRS is often characterized by a spectral signal-to-noise ratio (SNR) that varies highly between experiments. A common design for spectroscopic studies is to compare the ratio of two spectral peak amplitudes between groups, e.g. individual PCr/γ-ATP ratios in (31) P-MRS. The uncertainty on this ratio is often neglected. We wished to explore this assumption. THEORY: The canonical theory for the propagation of uncertainty on the ratio of two spectral peaks and its incorporation in the Frequentist hypothesis testing framework by weighted averaging is presented...
January 26, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28120242/improvement-of-ad-biosynthesis-response-to-enhanced-oxygen-transfer-by-oxygen-vectors-in-mycobacterium-neoaurum-tccc-11979
#17
Liqiu Su, Yanbing Shen, Tian Gao, Jianmei Luo, Min Wang
In steroid biotransformation, soybean oil can improve the productivity of steroids by increasing substrate solubility and strengthen the cell membrane permeability. However, little is known of its role as oxygen carrier and its mechanism of promoting the steroid biotransformation. In this work, soybean oil used as oxygen vector for the enhancement of androst-4-ene-3,17-dione (AD) production by Mycobacterium neoaurum TCCC 11979 (MNR) was investigated. Upon the addition of 16% (v/v) soybean oil, the volumetric oxygen transfer coefficient (K L a) value increased by 44%, and the peak molar yield of AD (55...
January 24, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28115150/mitochondrial-dysfunction-oxidative-stress-and-apoptotic-induction-in-microglial-bv-2-cells-treated-with-sodium-arsenate
#18
Wafa Kharroubi, Samia Haj Ahmed, Thomas Nury, Pierre Andreoletti, Rachid Sakly, Mohamed Hammami, Gérard Lizard
The treatment of microglial BV-2 cells with sodium arsenate (As(V): 0.1-400μmol/L - 48hr) induces a dose-dependent response. The neurotoxic effects of high concentrations of As(V) (100, 200 and 400μmol/L) are characterized by increased levels of mitochondrial complexes I, II, and IV followed by increased superoxide anion generation. Moreover, As(V) triggers an apoptotic mode of cell death, demonstrated by an apoptotic SubG1 peak, associated with an alteration of plasma membrane integrity. There is also a decrease in transmembrane mitochondrial potential and mitochondrial adenosine triphosphate ATP...
January 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28080323/oral-adenosine-5-triphosphate-atp-administration-increases-postexercise-atp-levels-muscle-excitability-and-athletic-performance-following-a-repeated-sprint-bout
#19
Martin Purpura, John A Rathmacher, Matthew H Sharp, Ryan P Lowery, Kevin A Shields, Jeremy M Partl, Jacob M Wilson, Ralf Jäger
OBJECTIVE: Oral adenosine-5'-triphosphate (ATP) administration has failed to increase plasma ATP levels; however, chronic supplementation with ATP has shown to increase power, strength, lean body mass, and blood flow in trained athletes. The purpose of this study was to investigate the effects of ATP supplementation on postexercise ATP levels and on muscle activation and excitability and power following a repeated sprint bout. METHODS: In a double-blind, placebo-controlled, randomized design, 42 healthy male individuals were given either 400 mg of ATP as disodium salt or placebo for 2 weeks prior to an exercise bout...
January 12, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28054274/nicorandil-improves-post-fatigue-tension-in-slow-skeletal-muscle-fibers-by-modulating-glutathione-redox-state
#20
E Sánchez-Duarte, X Trujillo, C Cortés-Rojo, A Saavedra-Molina, G Camargo, L Hernández, M Huerta, R Montoya-Pérez
Fatigue is a phenomenon in which force reduction has been linked to impairment of several biochemical processes. In skeletal muscle, the ATP-sensitive potassium channels (KATP) are actively involved in myoprotection against metabolic stress. They are present in sarcolemma and mitochondria (mitoKATP channels). K(+) channel openers like nicorandil has been recognized for their ability to protect skeletal muscle from ischemia-reperfusion injury, however, the effects of nicorandil on fatigue in slow skeletal muscle fibers has not been explored, being the aim of this study...
January 4, 2017: Journal of Bioenergetics and Biomembranes
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