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https://www.readbyqxmd.com/read/29407107/protection-against-reperfusion-injury-by-3-4-dihydroxyflavonol-in-rat-isolated-hearts-involves-inhibition-of-phospholamban-and-jnk2
#1
Kai Yee Chin, Lokugan S Silva, Ian A Darby, Dominic C H Ng, Owen L Woodman
BACKGROUND: Flavonols, including 3',4'-dihydroxyflavonol (DiOHF), reduce myocardial ischemia and reperfusion (I/R) injury but their mechanism remains uncertain. To better understand the mechanism of the cardioprotective actions of flavonols we investigated the effect of DiOHF on cardiac function and the activation of protective and injurious signalling kinases after I/R in rat isolated hearts. METHODS: We assessed the effect of global ischemia (20min) and reperfusion (5-30min) on cardiac function and injury in rat isolated, perfused hearts in the absence or presence of DiOHF (10μM) during reperfusion...
March 1, 2018: International Journal of Cardiology
https://www.readbyqxmd.com/read/29368427/physical-activity-unveils-the-relationship-between-mitochondrial-energetics-muscle-quality-and-physical-function-in-older-adults
#2
Giovanna Distefano, Robert A Standley, Xiaolei Zhang, Elvis A Carnero, Fanchao Yi, Heather H Cornnell, Paul M Coen
BACKGROUND: The concept of mitochondrial dysfunction in ageing muscle is highly controversial. In addition, emerging evidence suggests that reduced muscle oxidative capacity and efficiency underlie the aetiology of mobility loss in older adults. Here, we hypothesized that studying well-phenotyped older cohorts across a wide range of physical activity would unveil a range of mitochondrial function in skeletal muscle and in turn allow us to more clearly examine the impact of age per se on mitochondrial energetics...
January 24, 2018: Journal of Cachexia, Sarcopenia and Muscle
https://www.readbyqxmd.com/read/29351413/mitochondrial-content-is-preserved-throughout-disease-progression-in-the-mdx-mouse-model-of-duchenne-muscular-dystrophy-regardless-of-taurine-supplementation
#3
Robert G Barker, Victoria L Wyckelsma, Hongyang Xu, Robyn M Murphy
Mitochondrial dysfunction is a pathological feature of Duchenne muscular Dystrophy (DMD), a debilitating and fatal neuromuscular disorder characterised by progressive muscle wasting and weakness. Mitochondria are a source of cellular ATP and involved in Ca2+ regulation and apoptotic signalling. Ameliorating aberrant mitochondrial function has therapeutic potential for reducing DMD disease severity. The dystrophic mdx mouse exhibits peak muscle damage at 21-28d which stabilises after 8 weeks. The amino acid taurine is implicated in mitochondrial health and function, with endogenous concentrations low when measured during the cycle of peak muscle damage in mdx mice...
December 20, 2017: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29349673/pharmacological-dissection-of-the-cellular-mechanisms-associated-to-the-spontaneous-and-the-mechanically-stimulated-atp-release-by-mesentery-endothelial-cells-roles-of-thrombin-and-trpv
#4
M Verónica Donoso, Felipe Hernández, Tania Villalón, Claudio Acuña-Castillo, J Pablo Huidobro-Toro
Endothelial cells participate in extracellular ATP release elicited by mechanosensors. To characterize the dynamic interactions between mechanical and chemical factors that modulate ATP secretion by the endothelium, we assessed and compared the mechanisms participating in the spontaneous (basal) and mechanically stimulated secretion using primary cultures of rat mesentery endothelial cells. ATP/metabolites were determined in the cell media prior to (basal) and after cell media displacement or a picospritzer buffer puff used as mechanical stimuli...
January 19, 2018: Purinergic Signalling
https://www.readbyqxmd.com/read/29244215/development-of-a-validated-liquid-chromatographic-method-for-quantification-of-sorafenib-tosylate-in-the-presence-of-stress-induced-degradation-products-and-in-biological-matrix-employing-analytical-quality-by-design-aqbd-approach
#5
Teenu Sharma, Rajneet Kaur Khurana, Atul Jain, O P Katare, Bhupinder Singh
The current research work envisages AQbD-enabled development of a simple, rapid, sensitive, specific, robust and cost-effective stability indicating reversed phase high performance liquid chromatographic (RP-HPLC) method for determining stress-induced forced degradation products of sorafenib tosylate (SFN). An Ishikawa fish bone diagram was constructed to embark upon analytical target profile (ATP) and critical analytical attributes (CAAs) i.e., peak area, theoretical plates, retention time (Rt ) and peak tailing...
December 15, 2017: Biomedical Chromatography: BMC
https://www.readbyqxmd.com/read/29239276/radiolabeled-anti-adenosine-triphosphate-synthase-monoclonal-antibody-as-a-theragnostic-agent-targeting-angiogenesis
#6
Bok-Nam Park, Su Jin Lee, Jung Hyun Roh, Kyung-Han Lee, Young-Sil An, Joon-Kee Yoon
INTRODUCTION: The potential of a radioiodine-labeled, anti-adenosine triphosphate synthase monoclonal antibody (ATPS mAb) as a theragnostic agent for simultaneous cancer imaging and treatment was evaluated. METHODS: Adenosine triphosphate synthase monoclonal antibody was labeled with radioiodine, then radiotracer uptake was measured in 6 different cancer cell lines. In vivo biodistribution was evaluated 24 and 48 hours after intravenous injection of 125I-ATPS mAb into MKN-45 tumor-bearing mice (n = 3)...
January 2017: Molecular Imaging
https://www.readbyqxmd.com/read/29198136/hyperhomocysteinemia-induced-gene-expression-changes-in-the-cell-types-of-the-brain
#7
Erica M Weekman, Abigail E Woolums, Tiffany L Sudduth, Donna M Wilcock
High plasma levels of homocysteine, termed hyperhomocysteinemia, are a risk factor for vascular cognitive impairment and dementia, which is the second leading cause of dementia. While hyperhomocysteinemia induces microhemorrhages and cognitive decline in mice, the specific effect of hyperhomocysteinemia on each cell type remains unknown. We took separate cultures of astrocytes, microglia, endothelial cells, and neuronal cells and treated each with moderate levels of homocysteine for 24, 48, 72, and 96 hr...
November 2017: ASN Neuro
https://www.readbyqxmd.com/read/29196144/spatio-temporal-expression-of-hexokinase-3-in-the-injured-female-rat-spinal-cords
#8
Yu-Hong Lin, Yan Wu, Ying Wang, Zong-Feng Yao, Jie Tang, Rui Wang, Lin Shen, Shu-Qin Ding, Jian-Guo Hu, He-Zuo Lü
Hexokinase-3 (HK3) is a member of hexokinase family, which can catalyze the first step of glucose metabolism. It can increase ATP levels, reduce the production of reactive oxygen species, increase mitochondrial biogenesis, protect mitochondrial membrane potential and play an antioxidant role. However, the change of its expression in spinal cord after injury is still unknown. In this study, we investigated the spatio-temporal expression of HK3 in the spinal cords by using a spinal cord injury (SCI) model in adult female Sprague-Dawley rats...
November 28, 2017: Neurochemistry International
https://www.readbyqxmd.com/read/29193800/suppression-of-circadian-secretion-of-glucagon-like-peptide-1-by-the-saturated-fatty-acid-palmitate
#9
A Martchenko, R H Oh, S E Wheeler, P Gurges, J A Chalmers, P L Brubaker
AIM: Glucagon-like peptide-1 is an incretin hormone secreted by the intestinal L-cell with a circadian rhythm that parallels expression of the core clock gene, Bmal1. Although feeding rats a high-fat/high-sucrose Western diet impairs rhythmic glucagon-like peptide-1 release, the mechanisms underlying this effect remain unclear. Therefore, the aim of the present study was to determine the pathway(s) by which the saturated fat, palmitate, a major component of the Western diet, impairs circadian glucagon-like peptide-1 secretion...
November 29, 2017: Acta Physiologica
https://www.readbyqxmd.com/read/29192919/oxidative-stress-induced-increase-of-intracellular-zinc-in-astrocytes-decreases-their-functional-expression-of-p2x7-receptors-and-engulfing-activity
#10
Takahiro Furuta, Ayumi Mukai, Akihiro Ohishi, Kentaro Nishida, Kazuki Nagasawa
Neuron-glia communication mediated by neuro- and glio-transmitters such as ATP and zinc is crucial for the maintenance of brain homeostasis, and its dysregulation is found under pathological conditions. It is reported that under oxidative stress-loaded conditions, astrocytes exhibit increased intra- and extra-cellular labile zinc, the latter triggering microglial M1 activation, while the pathophysiological role of the former remains unrevealed. In this study, we examined whether the oxidative stress-induced increase of intracellular labile zinc is involved in the P2X7 receptor (P2X7R)-mediated regulation of astrocytic engulfing activity...
December 1, 2017: Metallomics: Integrated Biometal Science
https://www.readbyqxmd.com/read/29185963/the-therapeutic-nuclear-magnetic-resonance-changes-the-balance-in-intracellular-calcium-and-reduces-the-interleukin-1%C3%AE-induced-increase-of-nf-%C3%AE%C2%BAb-activity-in-chondrocytes
#11
Bibiane Steinecker-Frohnwieser, Werner Kullich, Anda Mann, Hans Georg Kress, Lukas Weigl
OBJECTIVES: Osteoarthritis as the main chronic joint disease is characterised by the destruction of articular cartilage. Developing new, more effective and in particular non-invasive methods to achieve pain reduction of OA patients are of exceptional interest. Clinical observations demonstrated positive effects of therapeutically applied low nuclear magnetic resonance (NMRT) for the treatment of painful disorders of the musculoskeletal system. In this study the cellular mechanism of action of NMRT was examined on chondrocytes...
November 28, 2017: Clinical and Experimental Rheumatology
https://www.readbyqxmd.com/read/29167117/effect-of-6-weeks-of-high-intensity-one-legged-cycling-on-functional-sympatholysis-and-atp-signaling-in-patients-with-heart-failure
#12
Gregers Wibe Munch, Ulrik Winning Iepsen, Camilla Koch Ryrsø, Jaya Birgitte Rosenmeier, Bente Klarlund Pedersen, Stefan P Mortensen
Breathlessness during daily activities is the primary symptom in patients with heart failure (HF). Poor correlation between the hemodynamic parameters of left ventricular performance and perceived symptoms suggests that other factors such as skeletal muscle function plays a role in determining exercise capacity. We investigated the effect of six weeks of high-intensity one-legged cycling (HIC; 8x4 at 90% one-legged cycling max) on: 1) the ability to override sympathetic vasoconstriction (arterial infusion of tyramine) during one-legged knee-extensor exercise (KEE); 2) vascular function (arterial infusion of ACh, SNP, tyramine and ATP); 3) exercise capacity in HF patients with reduced ejection fraction (n=8) compared with healthy individuals (n=6)...
November 22, 2017: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29163045/down-regulation-of-ntpdase2-and-adp-sensitive-p2-purinoceptors-correlate-with-severity-of-symptoms-during-experimental-autoimmune-encephalomyelitis
#13
Marija Jakovljevic, Irena Lavrnja, Iva Bozic, Danijela Savic, Ivana Bjelobaba, Sanja Pekovic, Jean Sévigny, Nadezda Nedeljkovic, Danijela Laketa
The present study explores tissue and cellular distribution of ectonucleoside triphosphate diphosphohydrolase 2 (NTPDase2) and the gene and protein expression in rat spinal cord during the course of experimental autoimmune encephalomyelitis (EAE). Given that NTPDase2 hydrolyzes ATP with a transient accumulation of ADP, the expression of ADP-sensitive P2 purinoceptors was analyzed as well. The autoimmune disease was actively induced in Dark Agouti female rats and the changes were analyzed 10, 15 and 29 days after the induction...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/29154196/analysis-of-potential-genotoxic-impurities-in-rabeprazole-active-pharmaceutical-ingredient-via-liquid-chromatography-tandem-mass-spectrometry-following-quality-by-design-principles-for-method-development
#14
Katerina Iliou, Anđelija Malenović, Yannis L Loukas, Yannis Dotsikas
A novel Liquid Chromatography-tandem mass spectrometry (LC-MS/MS) method is presented for the quantitative determination of two potential genotoxic impurities (PGIs) in rabeprazole active pharmaceutical ingredient (API). In order to overcome the analytical challenges in the trace analysis of PGIs, a development procedure supported by Quality-by-Design (QbD) principles was evaluated. The efficient separation between rabeprazole and the two PGIs in the shortest analysis time was set as the defined analytical target profile (ATP) and to this purpose utilization of a switching valve allowed the flow to be sent to waste when rabeprazole was eluted...
November 12, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29121518/genome-wide-identification-phylogenetic-analysis-and-expression-profiles-of-atp-binding-cassette-transporter-genes-in-the-oriental-fruit-fly-bactrocera-dorsalis-hendel-diptera-tephritidae
#15
Lin-Fan Xiao, Wei Zhang, Tian-Xing Jing, Meng-Yi Zhang, Ze-Qing Miao, Dan-Dan Wei, Guo-Rui Yuan, Jin-Jun Wang
The ATP-binding cassette (ABC) is the largest transporter gene family and the genes play key roles in xenobiotic resistance, metabolism, and development of all phyla. However, the specific functions of ABC gene families in insects is unclear. We report a genome-wide identification, phylogenetic, and transcriptional analysis of the ABC genes in the oriental fruit fly, Bactrocera dorsalis (Hendel). We identified a total of 47 ABC genes (BdABCs) from the transcriptomic and genomic databases of B. dorsalis and classified these genes into eight subfamilies (A-H), including 7 ABCAs, 7 ABCBs, 9 ABCCs, 2 ABCDs, 1 ABCE, 3 ABCFs, 15 ABCGs, and 3 ABCHs...
October 10, 2017: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29109680/diversity-of-evoked-astrocyte-ca-2-dynamics-quantified-through-experimental-measurements-and-mathematical-modeling
#16
Marsa Taheri, Gregory Handy, Alla Borisyuk, John A White
Astrocytes are a major cell type in the mammalian brain. They are not electrically excitable, but generate prominent Ca(2+) signals related to a wide variety of critical functions. The mechanisms driving these Ca(2+) events remain incompletely understood. In this study, we integrate Ca(2+) imaging, quantitative data analysis, and mechanistic computational modeling to study the spatial and temporal heterogeneity of cortical astrocyte Ca(2+) transients evoked by focal application of ATP in mouse brain slices...
2017: Frontiers in Systems Neuroscience
https://www.readbyqxmd.com/read/29092799/novel-drosophila-model-for-psychiatric-disorders-including-autism-spectrum-disorder-by-targeting-of-atp-binding-cassette-protein-a
#17
Ibuki Ueoka, Hitoshi Kawashima, Atsushi Konishi, Mikio Aoki, Ryo Tanaka, Hideki Yoshida, Toru Maeda, Mamiko Ozaki, Masamitsu Yamaguchi
Autism spectrum disorder (ASD) is characterized by persistent deficits in social communication and social interactions, as well as restricted, stereotyped patterns of behavior and interests. In addition, alterations in circadian sleep-wake rhythm are common in young children with ASD. Mutations in ATP binding cassette subfamily A member 13 (ABCA13) have been recently identified in a monkey that displays behavior associated with ASD. ABCA13, a member of the ABCA family of proteins, is predicted to transport lipid molecules and is expressed in the human trachea, testis, bone marrow, hippocampus, cortex, and other tissues...
October 29, 2017: Experimental Neurology
https://www.readbyqxmd.com/read/29059240/formation-of-the-chaperonin-complex-studied-by-2d-nmr-spectroscopy
#18
Toshio Takenaka, Takashi Nakamura, Saeko Yanaka, Maho Yagi-Utsumi, Mahesh S Chandak, Kazunobu Takahashi, Subhankar Paul, Koki Makabe, Munehito Arai, Koichi Kato, Kunihiro Kuwajima
We studied the interaction between GroES and a single-ring mutant (SR1) of GroEL by the NMR titration of 15N-labeled GroES with SR1 at three different temperatures (20, 25 and 30°C) in the presence of 3 mM ADP in 100 mM KCl and 10 mM MgCl2 at pH 7.5. We used SR1 instead of wild-type double-ring GroEL to precisely control the stoichiometry of the GroES binding to be 1:1 ([SR1]:[GroES]). Native heptameric GroES was very flexible, showing well resolved cross peaks of the residues in a mobile loop segment (residue 17-34) and at the top of a roof hairpin (Asn51) in the heteronuclear single quantum coherence spectra...
2017: PloS One
https://www.readbyqxmd.com/read/29058110/computational-prediction-of-the-effects-of-the-intra-aortic-balloon-pump-on-heart-failure-with-valvular-regurgitation-using-a-3d-cardiac-electromechanical-model
#19
Chang-Hyun Kim, Kwang-Soup Song, Natalia A Trayanova, Ki Moo Lim
Intra-aortic balloon pump (IABP) is normally contraindicated in significant aortic regurgitation (AR). It causes and aggravates pre-existing AR while performing well in the event of mitral regurgitation (MR). Indirect parameters, such as the mean systolic pressure, product of heart rate and peak systolic pressure, and pressure-volume are used to quantify the effect of IABP on ventricular workload. However, to date, no studies have directly quantified the reduction in workload with IABP. The goal of this study is to examine the effect of IABP therapy on ventricular mechanics under valvular insufficiency by using a computational model of the heart...
October 23, 2017: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29052402/-effect-of-qibai-pingfei-capsule-medicated-serum-on-protein-expressions-of-katp-channel-in-pulmonary-arterial-smooth-muscle-cells-via-nitric-oxide
#20
Jie Zhu, Ze-Geng Li, Bao-Qin Wang, Xiang-Li Tong, Qing-He Peng
To investigate the ATP-sensitive potassium channel (KATP channel) protein expressions during different periods under hypoxia condition and explore the effect of Qibai Pingfei capsule medicated serum (hereinafter referred to as QBPF) on the correlation between the protein expressions of KATP channel and nitric oxide in rat pulmonary arterial smooth muscle cells(PASMCs). Qibai Pingfei capsules were given to SD rats via continuous gavage for 10 days to obtain QBPF. Primary rats PASMCs were cultured by the direct adherent culture method...
April 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
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