Read by QxMD icon Read

Peak Atp

Mario Encinar, Santiago Casado, Alicia Calzado-Martín, P Natale, Álvaro San Paulo, Montserrat Calleja, Marisela Vélez, Francisco Monroy, Iván López-Montero
Erythrocyte membranes have been particularly useful as a model for studies of membrane structure and mechanics. Native erythroid membranes can be electroformed as giant unilamellar vesicles (eGUVs). In the presence of ATP, the erythroid membrane proteins of eGUVs rearrange into protein networks at the microscale. Here, we present a detailed nanomechanical study of individual protein microfilaments forming the protein networks of eGUVs when spread on supporting surfaces. Using Peak Force tapping Atomic Force Microscopy (PF-AFM) in liquid environment we have obtained the mechanical maps of the composite lipid-protein networks supported on solid surface...
October 13, 2016: Colloids and Surfaces. B, Biointerfaces
Haifang Zhang, Yunxia Zhu, Xiaofang Xie, Min Wang, Hong Du, Shungao Xu, Ying Zhang, Mingyu Gong, Bin Ni, Huaxi Xu, Xinxiang Huang
The linear plasmid pBSSB1 mediates the flagellar phase variation in H:z66 positive Salmonella enterica serovar Typhi (S. Typhi). The gene named stp17 (S. Typhi plasmid number 17 gene) is located on pBSSB1 and encodes the protein STP17. The expression pattern at the protein-level and function of STP17 remains unknown. In this study, the recombinant protein STP17His6 was expressed, purified and used to prepare the polyclonal anti-STP17 antibody. We detected protein-level expression of stp17 in S. Typhi and further investigated the protein expression characteristics of stp17 in different growth phases by western blot analysis...
2016: Frontiers in Cellular and Infection Microbiology
Yuguang Wang, Ying-Ying Huang, Yong Wang, Peijun Lyu, Michael R Hamblin
Photobiomodulation (PBM) using red or near-infrared (NIR) light has been used to stimulate the proliferation and differentiation of adipose-derived stem cells. The use of NIR wavelengths such as 810nm is reasonably well accepted to stimulate mitochondrial activity and ATP production via absorption of photons by cytochrome c oxidase. However, the mechanism of action of 980nm is less well understood. Here we study the effects of both wavelengths (810nm and 980nm) on adipose-derived stem cells in vitro. Both wavelengths showed a biphasic dose response, but 810nm had a peak dose response at 3J/cm(2) for stimulation of proliferation at 24h, while the peak dose for 980nm was 10-100 times lower at 0...
October 15, 2016: Biochimica et Biophysica Acta
Yasuo Mitani, Ryo Futahashi, Zichao Liu, Xingcai Liang, Yoshihiro Ohmiya
Fireflies are widespread all over the world and a numerous numbers of luciferases have been isolated and characterized. In this study, we identified and characterized the luciferase and luciferase-like genes from a Tibetan firefly collected in Shangri-La, China. The altitude of this area is more than 3,300 meters. We saw this Tibetan firefly flying with strong luminescence after sunset at ~10°C. We analyzed the transcriptome of Tibetan firefly using head, thorax, abdomen (without light organ), and light organ tissue by RNA sequencing...
September 26, 2016: Photochemistry and Photobiology
M E Quinta-Ferreira, F D S Sampaio Dos Aidos, C M Matias, P J Mendes, J C Dionísio, R M Santos, L M Rosário, R M Quinta-Ferreira
Zinc, a transition metal existing in very high concentrations in the hippocampal mossy fibers from CA3 area, is assumed to be co-released with glutamate and to have a neuromodulatory role at the corresponding synapses. The synaptic action of zinc is determined both by the spatiotemporal characteristics of the zinc release process and by the kinetics of zinc binding to sites located in the cleft area, as well as by their concentrations. This work addresses total, free and complexed zinc concentration changes, in an individual synaptic cleft, following single, short and long periods of evoked zinc release...
October 1, 2016: Journal of Computational Neuroscience
Jianyu He, Junru Wang, Mengshan Xu, Changwen Wu, Huihui Liu
Heat shock proteins (HSPs) play significant roles in the immune response of fish in defending against diverse environmental threats or stresses. In this study, two complete HSP70 and HSP90 genes of Larimichthys crocea (designated as LycHSP70 and LycHSP90) were identified and characterized (GenBank accession no. KT456551 and KT456552). The complete open reading frame (ORF) fragments of LycHSP70 and LycHSP90 were 1917 bp and 2151 bp, encoding 638 and 716 amino acids residues respectively. Many significant functional domains and motifs were found, such as Hsp70 family signatures, Hsp90 family signatures, ATP-GTP binding site and EEVD motif regions, and they were associated with relative functions...
September 24, 2016: Fish & Shellfish Immunology
J Vissing
Metabolic myopathies encompass muscle glycogenoses (GSD) and disorders of muscle fat oxidation (FAOD). FAODs and GSDs can be divided into two main clinical phenotypes; those with static symptoms related to fixed muscle weakness and atrophy, and those with dynamic, exercise-related symptoms that are brought about by a deficient supply of ATP. Together with mitochondrial myopathies, metabolic myopathies are unique among muscle diseases, as the limitation in exercise performance is not solely caused by structural damage of muscle, but also or exclusively related to energy deficiency...
October 2016: Revue Neurologique
Rohan S Wijesurendra, Alexander Liu, Christian Eichhorn, Rina Ariga, Eylem Levelt, William T Clarke, Christopher T Rodgers, Theodoros D Karamitsos, Yaver Bashir, Matthew Ginks, Kim Rajappan, Tim Betts, Vanessa M Ferreira, Stefan Neubauer, Barbara Casadei
BACKGROUND: Lone atrial fibrillation (AF) may reflect a subclinical cardiomyopathy that persists after sinus rhythm (SR) restoration, providing a substrate for AF recurrence. To test this hypothesis, we investigated the effect of restoring SR by catheter ablation on left ventricular (LV) function and energetics in patients with AF but no significant comorbidities. METHODS: Fifty-three patients with symptomatic paroxysmal or persistent AF and without significant valvular disease, uncontrolled hypertension, coronary artery disease, uncontrolled thyroid disease, systemic inflammatory disease, diabetes mellitus, or obstructive sleep apnea (ie, lone AF) undergoing ablation and 25 matched control subjects in SR were investigated...
October 11, 2016: Circulation
Rui Li, Frederik J Steyn, Michael B Stout, Kevin Lee, Tanya R Cully, Juan C Calderón, Shyuan T Ngo
Metabolic dysfunction in skeletal muscle contributes to the aetiology and development of muscle diseases and metabolic diseases. As such, assessment of skeletal muscle cellular bioenergetics provides a powerful means to understand the role of skeletal muscle metabolism in disease and to identify possible therapeutic targets. Here, we developed a method that allows for the real-time assessment of cellular respiration in intact skeletal muscle fibre bundles obtained from the extensor digitorum longus (EDL) muscle of adult mice...
September 13, 2016: Journal of Physiology
Ankush Madaan, Mathieu Nadeau-Vallée, Jose Carlos Rivera, Dima Obari, Xin Hou, Estefania Marin Sierra, Sylvie Girard, David M Olson, Sylvain Chemtob
BACKGROUND: Uterine inflammatory processes trigger pro-labor pathways and orchestrate on-time labor onset. Although essential for successful labor, inflammation needs to be regulated to avoid uncontrolled amplification and resolve post-partum. During labor, myometrial smooth muscle cells generate ATP mainly via anaerobic glycolysis, resulting in accumulation of lactate. Aside from its metabolic function, lactate has been shown to activate a G protein-coupled receptor, GPR81, reported to regulate inflammation...
September 8, 2016: American Journal of Obstetrics and Gynecology
Sae Young Jae, Barry A Franklin, Arno Schmidt-Trucksass, Do Kyung Kim, Yoon-Ho Choi, Jeong Bae Park
We tested the hypothesis that cardiometabolic syndrome (CMS) is associated with subclinical atherosclerosis in men and that moderate-to-high levels of cardiorespiratory fitness (fitness) attenuate this relation. Our study population (n = 2,107 men) participated in a health screening program that included measures of coronary artery calcification (CAC) and carotid artery intima-media thickness (CIMT) as surrogate markers of subclinical atherosclerosis. The prevalence of subclinical atherosclerosis was defined as a CAC score >0 and a mean CIMT more than the seventy-fifth percentile...
August 13, 2016: American Journal of Cardiology
Sarah Zimmermann, Sarah Gretzinger, Marie-Luise Schwab, Christian Scheeder, Philipp K Zimmermann, Stefan A Oelmeier, Eric Gottwald, Are Bogsnes, Mattias Hansson, Arne Staby, Jürgen Hubbuch
As the clinical development of cell-based therapeutics has evolved immensely within the past years, downstream processing strategies become more relevant than ever. Aqueous two-phase systems (ATPS) enable the label-free, scalable, and cost-effective separation of cells, making them a promising tool for downstream processing of cell-based therapeutics. Here, we report the development of an automated robotic screening that enables high-throughput cell partitioning analysis in ATPS. We demonstrate that this setup enables fast and systematic investigation of factors influencing cell partitioning...
September 16, 2016: Journal of Chromatography. A
Brian D Fink, Fan Bai, Liping Yu, William I Sivitz
Recently, we used an ADP recycling approach to examine mouse skeletal muscle (SkM) mitochondrial function over respiratory states intermittent between state 3 and 4. We showed that respiration energized at complex II by succinate, in the presence of rotenone to block complex I, progressively increased with incremental additions of ADP. However, in the absence of rotenone, respiration peaked at low [ADP] but then dropped markedly as [ADP] was further increased. Here, we tested the hypothesis that these respiratory dynamics would differ between mitochondria of mice fed high-fat (HF) and treated with a low dose of streptozotocin to mimic type 2 diabetes and mitochondria from controls...
August 24, 2016: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
Alistair V W Nunn, Geoffrey W Guy, Jimmy D Bell
A sufficiently complex set of molecules, if subject to perturbation, will self-organize and show emergent behaviour. If such a system can take on information it will become subject to natural selection. This could explain how self-replicating molecules evolved into life and how intelligence arose. A pivotal step in this evolutionary process was of course the emergence of the eukaryote and the advent of the mitochondrion, which both enhanced energy production per cell and increased the ability to process, store and utilize information...
August 15, 2016: Biochemical Society Transactions
Chaoguang Wang, Xuezhong Wu, Peitao Dong, Jian Chen, Rui Xiao
Paraquat (PQ) pollutions are ultra-toxic to human beings and hard to be decomposed in the environment, thus requiring an on-site detection strategy. Herein, we developed a robust and rapid PQ sensing strategy based on the surface-enhanced Raman scattering (SERS) technique. A hybrid SERS substrate was prepared by grafting the Au@Ag core-shell nanoparticles (NPs) on the Au film over slightly etched nanoparticles (Au FOSEN). Hotspots were engineered at the junctions as indicated by the finite difference time domain calculation...
December 15, 2016: Biosensors & Bioelectronics
Ayşem Üzer, Şener Sağlam, Ziya Can, Erol Erçağ, Reşat Apak
Due to the negative impact of nitrate and nitrite on human health, their presence exceeding acceptable levels is not desired in foodstuffs. Thus, nitrite determination at low concentrations is a major challenge in electroanalytical chemistry, which can be achieved by fast, cheap, and safe electrochemical sensors. In this work, the working electrode (Au) was functionalized with p-aminothiophenol (p-ATP) and modified with gold nanoparticles (Au-NPs) to manufacture the final (Au/p-ATP-Aunano) electrode in a two-step procedure...
2016: International Journal of Molecular Sciences
Reiko Murakami, Risa Mutoh, Ketaro Ishii, Masahiro Ishiura
The circadian clock is an endogenous biological mechanism that generates autonomous daily cycles in physiological activities. The phosphorylation levels of KaiC oscillated with a period of 24 h in an ATP-dependent clock oscillator reconstituted in vitro from KaiA, KaiB and KaiC. We examined the complex formations of KaiA and KaiB with KaiC in the KaiABC clock oscillator by fluorescence correlation spectrometry (FCS) analysis. The formation of KaiB-containing protein complex(es) oscillated in a circadian manner, with a single peak at 12 h and single trough at 24 h in the circadian cycle, whereas that of KaiA-containing protein complex(es) oscillated with two peaks at 12 and 24 h...
August 2016: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Danilo T Amaral, Gabriela Oliveira, Jaqueline R Silva, Vadim R Viviani
Bioluminescent click-beetles display a wide variation of bioluminescence colors ranging from green to orange, including an unusual intra-specific color variation in the Jamaican Pyrophorus plagiophthalamus. Recently, we collected individuals of the Pyrophorus angustus species from the Southern Amazon forest, in Brazil, which displays an orange light emitting abdominal lantern. This species was also previously described from Central America, but displaying a bioluminescence spectrum from 536 nm (dorsal) to 578 nm (ventral)...
September 2016: Photochemical & Photobiological Sciences
Jingyu Liu, Tino Prell, Beatrice Stubendorff, Silke Keiner, Thomas Ringer, Anne Gunkel, Vedrana Tadic, Nadine Goldhammer, Ayse Malci, Otto W Witte, Julian Grosskreutz
BACKGROUND: Amyotrophic lateral sclerosis (ALS) is a neurodegenerative disorder associated with intracellular Ca(2+) dysregulation. The P2X receptor family is comprised of ligand-gated ion channels that respond to extracellular adenosine triphosphate (ATP) and increases permeability of calcium into the cell. The underlying mechanisms of purinergic signalling on peripheral blood mononuclear cells (PBMCs) in ALS remain unclear. Herein, we studied the expression of P2X4/P2X7 receptors and calcium homeostasis in blood cells of ALS patients...
September 6, 2016: Neuroscience Letters
Joel P Giblett, Richard G Axell, Paul A White, Sophie J Clarke, Liam McCormick, Philip A Read, Johannes Reinhold, Adam J Brown, Michael O'Sullivan, Nick E J West, David P Dutka, Stephen P Hoole
BACKGROUND: Glucagon-like peptide-1 (7-36) amide (GLP-1) protects against stunning and cumulative left ventricular dysfunction in humans. The mechanism remains uncertain but GLP-1 may act by opening mitochondrial K-ATP channels in a similar fashion to ischemic conditioning. We investigated whether blockade of K-ATP channels with glibenclamide abrogated the protective effect of GLP-1 in humans. METHODS: Thirty-two non-diabetic patients awaiting stenting of the left anterior descending artery (LAD) were allocated into 4 groups (control, glibenclamide, GLP-1, and GLP-1 + glibenclamide)...
2016: Cardiovascular Diabetology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"