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https://www.readbyqxmd.com/read/29786812/the-microrna-expression-profile-in-rat-lung-tissue-early-after-burn-injury
#1
Donghai Zhang, Yang Chang, Shaofang Han, Longlong Yang, Quan Hu, Yonghui Yu, Lingying Liu, Jiake Chai
BACKGROUND: Severe burn causes acute lung injury in many victims, but the related mechanisms have been barely investigated. microRNAs (miRNAs) important regulators in numerous physiological and pathophysiological process. However, the roles of miRNAs in burn lung injury are untested. METHODS: Six healthy male Sprague-Dawley rats were randomly assigned into burn and sham groups. Lung injury was evaluated by hematoxylin and eosin (HE) staining at 24 h after injury...
May 2018: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/29786744/mir%C3%A2-133b-regulates-proliferation-and-apoptosis-in-high%C3%A2-glucose%C3%A2-induced-human-retinal-endothelial-cells-by-targeting-ras-homolog-family-member-a
#2
Jun Yao, Jihong Wang, Yong Yao, Kelei Wang, Qianqian Zhou, Ying Tang
The aim of the present study was to investigate the role of microRNA (miR)‑133b in high‑glucose‑induced human retinal endothelial cells (hRECs), particularly regarding its potential targeting of ras homolog family member A (RhoA). To establish the high‑glucose‑induced diabetic retinopathy (DR) model, hRECs were cultured in high‑glucose medium for 1, 2 and 3 days. An Annexin allophycocyanin (APC)/7‑aminoactinomycin D (7‑AAD) staining assay was performed to measure the apoptosis of hRECs. Next, the cells were transfected with miR‑133b inhibitors or mimics, and the cell proliferation and apoptosis were measured by MTT and Annexin‑APC/7‑AAD staining assays, respectively...
May 18, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29785845/bromate-and-nitrate-bio-reduction-coupled-with-poly-%C3%AE-hydroxybutyrate-production-in-a-methane-based-membrane-biofilm-reactor
#3
Chun-Yu Lai, Pan-Long Lv, Qiu-Yi Dong, Shi Lei Yeo, Bruce E Rittmann, He-Ping Zhao
This work demonstrates bromate (BrO3-) reduction in a methane (CH4)-based membrane biofilm reactor (MBfR), and it documents contrasting impacts of nitrate (NO3-) on BrO3- reduction, as well as formation of poly--hydroxybutyrate (PHB), an internal C- and electron-storage material. When the electron donor, CH4, was in ample supply, NO3- enhanced BrO3- reduction by stimulating the growth of denitrifying bacteria (Meiothermus, Comamonadaceae, and Anaerolineaceae) able to reduce BrO3- and NO3- simultaneously...
May 22, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29785740/expression-levels-of-breast-cancer-related-gas5-and-lsinct5-lncrnas-in-cancer-free-breast-tissue-molecular-associations-with-age-at-menarche-and-obesity
#4
Yaser Mansoori, Mohammad Bagher Tabei, Alireza Askari, Pantea Izadi, Abdolreza Daraei, Milad Bastami, Mohammad Mehdi Naghizadeh, Ziba Nariman-Saleh-Fam, Behnam Mansoori, Javad Tavakkoly-Bazzaz
Long noncoding RNAs (lncRNAs) constitute a major class of the human transcriptome which play crucial roles in the key biological processes of both normal and malignant breast cells. Although the aberrant expression of lncRNAs has been well-documented in breast cancer (BC), little is currently known about the association between their expression levels in the breast tissue of healthy women and BC risk factors, especially the reproductive or demographic characteristics that are among the most well-known BC risk modifiers...
May 21, 2018: Breast Journal
https://www.readbyqxmd.com/read/29785394/notch-signaling-pathway-is-inhibited-in-the-development-of-barrett-s-esophagus-an-in-vivo-and-in-vitro-study
#5
Yun-Cang Wang, Zhi-Qiang Wang, Yong Yuan, Tao Ren, Peng-Zhi Ni, Long-Qi Chen
Objective: To explore the role of Notch signaling in the development of Barrett's esophagus. Methods: Patients with esophagectomy and gastric interposition were recruited as a human model of gastroesophageal reflux disease. The expressions of Notch signaling genes in normal esophagus from surgical specimen and columnar metaplasia in the esophageal remnant after esophagectomy were evaluated by real time quantitative Polymerase Chain Reaction (RT-qPCR) and immunohistochemistry (IHC)...
2018: Canadian Journal of Gastroenterology & Hepatology
https://www.readbyqxmd.com/read/29785219/assessing-methanogenic-archaeal-community-in-full-scale-anaerobic-sludge-digester-systems-in-dubai-united-arab-emirates
#6
Munawwar A Khan, Poojabahen G Patel, Arpitha G Ganesh, Naushad Rais, Sultan M Faheem, Shams T Khan
Introduction: Anaerobic digestion for methane production comprises of an exceptionally diverse microbial consortium, a profound understanding about which is still constrained. In this study, the methanogenic archaeal communities in three full-scale anaerobic digesters of a Municipal Wastewater Treatment Plant were analyzed by Fluorescence in situ hybridization and quantitative real-time Polymerase Chain Reaction (qPCR) technique. Methods & Materials: Fluorescence in situ hybridization (FISH) was performed to detect and quantify the methanogenic Archaea in the sludge samples whereas qPCR was carried out to support the FISH analysis...
2018: Open Microbiology Journal
https://www.readbyqxmd.com/read/29785005/nanopore-sequencing-technology-a-new-route-for-the-fast-detection-of-unauthorized-gmo
#7
Marie-Alice Fraiture, Assia Saltykova, Stefan Hoffman, Raf Winand, Dieter Deforce, Kevin Vanneste, Sigrid C J De Keersmaecker, Nancy H C Roosens
In order to strengthen the current genetically modified organism (GMO) detection system for unauthorized GMO, we have recently developed a new workflow based on DNA walking to amplify unknown sequences surrounding a known DNA region. This DNA walking is performed on transgenic elements, commonly found in GMO, that were earlier detected by real-time PCR (qPCR) screening. Previously, we have demonstrated the ability of this approach to detect unauthorized GMO via the identification of unique transgene flanking regions and the unnatural associations of elements from the transgenic cassette...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29785002/using-single-molecule-mrna-fluorescent-in-situ-hybridization-rna-fish-to-quantify-mrnas-in-individual-murine-oocytes-and-embryos
#8
Fang Xie, Kelsey A Timme, Jennifer R Wood
Changes in abundance of mRNAs during oocyte growth and maturation and during pre-implantation embryo development have been documented using quantitative real-time RT-PCR (qPCR), microarray analyses, and whole genome sequencing. However, these techniques require amplification of mRNAs, normalization using housekeeping genes, can be biased for abundant transcripts, and/or require large numbers of oocytes and embryos which can be difficult to acquire from mammalian species. We optimized a single molecule RNA fluorescence in situ hybridization (RNA-FISH) protocol, which amplifies fluorescence signal to detect candidate transcripts, for use with individual oocytes and embryos...
May 21, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29784358/short-probe-based-duplex-specific-nuclease-signal-amplification-strategy-enables-imaging-of-endogenous-micrornas-in-living-cells-with-ultrahigh-specificity
#9
Yingjun Ma, Jun Chen, Danping Chen, Yuzhi Xu, Li Zhang, Zong Dai, Xiaoyong Zou
Specific nucleic acids amplification at a constant and mild temperature is important for imaging assay of endogenous microRNAs (miRNAs) in living cells. Duplex-specific nuclease (DSN) is attractive in one-step isothermal assay of miRNA; however, its intrinsic limitations of low amplification specificity and high reaction temperature greatly restrict the application scope. Herein, we present a short-probe-based DSN signal amplification (spDSNSA) strategy enabling analysis of miRNAs at body temperature with significantly high specificity...
August 15, 2018: Talanta
https://www.readbyqxmd.com/read/29784046/knockdown-of-annexin-a5-restores-gefitinib-sensitivity-by-promoting-g2-m-cell-cycle-arrest
#10
Jian Zhou, Meijia Chang, Jing Li, Tao Fang, Jie Hu, Chunxue Bai
BACKGROUND: Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors, including gefitinib, are first-line drugs against advanced non-small cell lung cancer with activating EGFR mutations. However, the development of resistance to such drugs is a major clinical challenge. METHODS: The role of annexin A5 in resistance to EGFR tyrosine kinase inhibitors was investigated by qPCR and western blot of relevant molecules, by CCK8 and EdU assay of cell proliferation and viability, by annexin V/propidium iodide assay of apoptosis and cell cycle distribution, by JC-1 assay of mitochondrial integrity, and by xenograft assay of tumorigenicity...
May 21, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29784043/dysregulation-of-mir-200s-clusters-as-potential-prognostic-biomarkers-in-acute-myeloid-leukemia
#11
Jing-Dong Zhou, Liu-Chao Zhang, Ting-Juan Zhang, Yu Gu, De-Hong Wu, Wei Zhang, Ji-Chun Ma, Xiang-Mei Wen, Hong Guo, Jiang Lin, Jun Qian
BACKGROUND: Increasing studies showed that miR-200 family (miR-200s) clusters are aberrantly expressed in multiple human cancers, and miR-200s clusters function as tumor suppressor genes by affecting cell proliferation, self-renewal, differentiation, division and apoptosis. Herein, we aimed to investigate the expression and clinical implication of miR-200s clusters in acute myeloid leukemia (AML). METHODS: RT-qPCR was performed to detect expression of miR-200s clusters in 19 healthy donors, 98 newly diagnosed AML patients, and 35 AML patients achieved complete remission (CR)...
May 21, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29783973/erythrocyte-microrna-sequencing-reveals-differential-expression-in-relapsing-remitting-multiple-sclerosis
#12
Kira Groen, Vicki E Maltby, Rodney A Lea, Katherine A Sanders, J Lynn Fink, Rodney J Scott, Lotti Tajouri, Jeannette Lechner-Scott
BACKGROUND: There is a paucity of knowledge concerning erythrocytes in the aetiology of Multiple Sclerosis (MS) despite their potential to contribute to disease through impaired antioxidant capacity and altered haemorheological features. Several studies have identified an abundance of erythrocyte miRNAs and variable profiles associated with disease states, such as sickle cell disease and malaria. The aim of this study was to compare the erythrocyte miRNA profile of relapsing-remitting MS (RRMS) patients to healthy sex- and age-matched controls...
May 21, 2018: BMC Medical Genomics
https://www.readbyqxmd.com/read/29783929/modulation-the-crosstalk-between-tumor-associated-macrophages-and-non-small-cell-lung-cancer-to-inhibit-tumor-migration-and-invasion-by-ginsenoside-rh2
#13
Honglin Li, Nan Huang, Weikang Zhu, Jianchun Wu, Xiaohui Yang, Wenjing Teng, Jianhui Tian, Zhihong Fang, Yingbin Luo, Min Chen, Yan Li
BACKGROUND: Tumor-associated macrophages (TAMs) play a critical role in modulating the tumor microenvironment and promote tumor metastases. Our studies have demonstrated that ginsenoside Rh2 (G-Rh2), a monomeric compound extracted from ginseng, is a promising anti-tumor agent in lung cancer cells. However, it remains unclear whetherG-Rh2 can modulate the differentiation of TAMs and its interaction with tumor microenvironment. In this study, we investigated how G-Rh2 regulates the phenotype of macrophages and affects the migration of non-small cell lung cancer (NSCLC) cells...
May 22, 2018: BMC Cancer
https://www.readbyqxmd.com/read/29783661/analysis-of-short-term-smoking-effects-in-pbmc-of-healthy-subjects-preliminary-study
#14
Joanna Wieczfinska, Tomasz Kowalczyk, Przemyslaw Sitarek, Ewa Skała, Rafal Pawliczak
Early structural changes exist in the small airways before the establishment of Chronic Obstructive Pulmonary Disease (COPD). These changes are believed to be induced by oxidation. The aim of this study was to analyze the influence of short-term smoking on the expression of the genes contributing to airway remodeling and their relationship with the oxidative status of human blood cells. Blood mononuclear cells were isolated from 16 healthy volunteers and treated with cigarette smoke ingredients (CSI): nicotine, 1-Nitrosodimethylamine, N-Nitrosopyrrolidyne, vinyl chloride, acetone, and acrolein...
May 18, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29783074/fsh-receptor-binding-inhibitor-restrains-follicular-development-and-possibly-attenuates-carcinogenesis-of-ovarian-cancer-through-down-regulating-expression-levels-of-fshr-and-er%C3%AE-in-normal-ovarian-tissues
#15
Gong Zhuandi, Che Tuanjie, Lai Luju, Ayimuguli Abdiryim, Deng Yingying, Liang Haoqin, Wei Suocheng, Ding Li
OBJECTIVES: The current study aimed to investigate FSH receptor binding inhibitor (FRBI) effects in the expressions of FSH receptor (FSHR) and estrogen receptor-beta (ERβ) in the mice ovaries at the gene and protein levels, also to find the potential efficacy of FRBI on suppressing ovarian cancer through down-regulating over-expression of FSHR and ERβ in the normal ovarian tissues. METHODS: 180 female mice were randomized into six groups (n = 30). Mice of FRBI-1, FRBI-2 and FRBI-3, FRBI-4 were intramuscularly injected with FRBI of 10, 20, 30 and 40 mg/kg, respectively, for five consecutive days...
May 18, 2018: Gene
https://www.readbyqxmd.com/read/29782871/neuralized1a-regulates-asymmetric-division-in-mouse-lewis-lung-carcinoma-cells
#16
Liangsheng Kong, Jingyuan Li, Yongli Liu, Zhiwei Sun, Shixia Zhou, Junling Tang, Ting Ye, Jianyu Wang, H Rosie Xing
Asymmetric division (ASD), the unique characteristic of normal stem cells, is regarded as a stemness marker when applied to the study of cancer stem cells (CSCs). However, the role of ASD in the self-renewal of CSCs and its regulation remain largely unknown. Here, we first established a mouse Lewis lung carcinoma CSC cell line that could undergo asymmetric division (LLC-ASD cells) derived from the parental mouse Lewis lung carcinoma cancer cells (LLC-Parental cells). In vitro assessment of stemness by RT-qPCR and western blot analysis of stem cell markers, clonogenic assay (p < 0...
May 18, 2018: Life Sciences
https://www.readbyqxmd.com/read/29782522/molecular-detection-of-mycobacterium-ulcerans-in-the-environment-and-its-relationship-with-buruli-ulcer-occurrence-in-zio-and-yoto-districts-of-maritime-region-in-togo
#17
Issaka Maman, Tchadjobo Tchacondo, Abiba Banla Kere, Marcus Beissner, Kossi Badziklou, Ekanao Tedihou, Edith Nyaku, Komi Amekuse, Franz Xaver Wiedemann, Damintoti Simplice Karou, Gisela Bretzel
BACKGROUND: Buruli Ulcer (BU) is a neglected tropical skin infection caused by Mycobacterium ulcerans. Residence near aquatic areas has been identified as an important source of transmission of M. ulcerans with increased risk of contracting Buruli ulcer. However, the reservoir and the mode of transmission are not yet well known. The aim of this study was to identify the presence of M. ulcerans in the environment and its relationship with Buruli ulcer occurrence in Zio and Yoto districts of the maritime region in south Togo...
May 21, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29782495/molecular-immunological-and-neurophysiological-evaluations-for-early-diagnosis-of-neural-impairment-in-seropositive-leprosy-household-contacts
#18
Diogo Fernandes Dos Santos, Matheus Rocha Mendonça, Douglas Eulálio Antunes, Elaine Fávaro Pípi Sabino, Raquel Campos Pereira, Luiz Ricardo Goulart, Isabela Maria Bernardes Goulart
BACKGROUND: Household contacts constitute the highest risk group for leprosy development, and despite significant progress in the disease control, early diagnosis remains the primary goals for leprosy management programs. METHODS: We have recruited 175 seropositive and 35 seronegative household contacts from 2014 to 2016, who were subjected to an extensive protocol that included clinical, molecular (peripheral blood qPCR, slit-skin smear qPCR, skin biopsy qPCR) and electroneuromyographic evaluations...
May 21, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29782349/lncrna-malat-1-competitively-regulates-mir-124-to-promote-emt-and-development-of-non-small-cell-lung-cancer
#19
Jiasheng Wu, Yueyue Weng, Fei He, Dong Liang, Lin Cai
This study was carried out to explore the mechanism of LncRNA MALAT-1 as a competing endogenous RNA to regulate miR-124 in epithelial-mesenchymal transition and development of non-small-cell lung cancer (NSCLC). NSCLC and adjacent tissues were collected for RT-qPCR. The correlation of MALAT-1 and miR-124 was analyzed by Pearson. MALAT-1 expression was measured in NSCLC A549, NCI-H460, NCI-H529, SK-MES-1 cells, and 16HBE cells. A549 cells were selected for cell transfection experiments after the creation of six groups...
May 18, 2018: Anti-cancer Drugs
https://www.readbyqxmd.com/read/29782025/injections-of-lipopolysaccharide-into-mice-to-mimic-entrance-of-microbial-derived-products-after-intestinal-barrier-breach
#20
Katarina Raduolovic, Rachel Mak'Anyengo, Berna Kaya, Anna Steinert, Jan Hendrik Niess
The intestinal epithelial barrier separates the host from the microbiota that is normally tolerated or ignored. The breach of this barrier results in the entrance of bacteria or bacteria-derived products into the host, accessing the host circulation and inner organs leading to the uncontrolled inflammation as observed in patients with inflammatory bowel disease (IBD), that are characterized by an increased intestinal epithelial permeability. To mimic the entrance of bacterial-derived compounds into the host, an endotoxemia model has been adopted in which lipopolysaccharide (LPS), a component of the outer cell wall of Gram-negative bacteria, were injected into mice...
May 2, 2018: Journal of Visualized Experiments: JoVE
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