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https://www.readbyqxmd.com/read/29346650/persistence-of-zika-virus-after-birth-clinical-virological-neuroimaging-and-neuropathological-documentation-in-a-5-month-infant-with-congenital-zika-syndrome
#1
Leila Chimelli, Sheila Moura Pone, Elyzabeth Avvad-Portari, Zilton Farias Meira Vasconcelos, Andrea Araújo Zin, Daniela Prado Cunha, Nathalia Raposo Thompson, Maria Elisabeth Lopes Moreira, Clayton A Wiley, Marcos Vinicius da Silva Pone
During the Zika epidemic in Brazil, a baby was born at term with microcephaly and arthrogryposis. The mother had Zika symptoms at 10 weeks of gestation. At 17 weeks, ultrasound showed cerebral malformation and ventriculomegaly. At 24 weeks, the amniotic fluid contained ZIKV RNA and at birth, placenta and maternal blood were also positive using RT-qPCR. At birth the baby urine contained ZIKV RNA, whereas CSF at birth and urine at 17 days did not. Seizures started at 6 days. EEG was abnormal and CT scan showed cerebral atrophy, calcifications, lissencephaly, ventriculomegaly, and cerebellar hypoplasia...
January 13, 2018: Journal of Neuropathology and Experimental Neurology
https://www.readbyqxmd.com/read/29346643/genetic-characterization-of-a-vang-type-vancomycin-resistant-enterococcus-faecium-clinical-isolate
#2
Mohamed Sassi, François Guérin, Léonie Lesec, Christophe Isnard, Marguerite Fines-Guyon, Vincent Cattoir, Jean-Christophe Giard
Objectives: To characterize, phenotypically and genotypically, the first Enterococcus faecium clinical isolate harbouring a vanG operon. Methods: The antibiotic resistance profile of E. faecium 16-346 was determined and its whole genome sequenced using PacBio technology. Attempts to transfer vancomycin resistance by filter mating were performed and the inducibility of expression of the vanG operon was studied by reverse-transcription quantitative PCR (RT-qPCR) in the presence or absence of subinhibitory concentrations of vancomycin...
January 16, 2018: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/29346438/colonization-of-the-cervicovaginal-space-with-gardnerella-vaginalis-leads-to-local-inflammation-and-cervical-remodeling-in-pregnant-mice
#3
Luz-Jeannette Sierra, Amy G Brown, Guillermo O Barilá, Lauren Anton, Carrie E Barnum, Snehal S Shetye, Louis J Soslowsky, Michal A Elovitz
The role of the cervicovaginal (CV) microbiome in regulating cervical function during pregnancy is poorly understood. Gardnerella vaginalis (G. vaginalis) is the most common bacteria associated with the diagnosis of bacterial vaginosis (BV). While BV has been associated with preterm birth (PTB), clinical trials targeting BV do not decrease PTB rates. It remains unknown if G. vaginalis is capable of triggering molecular, biomechanical and cellular events that could lead to PTB. The objective of this study was to determine if cervicovaginal colonization with G...
2018: PloS One
https://www.readbyqxmd.com/read/29346366/testing-for-soil-transmitted-helminth-transmission-elimination-analysing-the-impact-of-the-sensitivity-of-different-diagnostic-tools
#4
Marleen Werkman, James E Wright, James E Truscott, Alice V Easton, Rita G Oliveira, Jaspreet Toor, Alison Ower, Kristjana H Ásbjörnsdóttir, Arianna R Means, Sam H Farrell, Judd L Walson, Roy M Anderson
In recent years, an increased focus has been placed upon the possibility of the elimination of soil-transmitted helminth (STH) transmission using various interventions including mass drug administration. The primary diagnostic tool recommended by the WHO is the detection of STH eggs in stool using the Kato-Katz (KK) method. However, detecting infected individuals using this method becomes increasingly difficult as the intensity of infection decreases. Newer techniques, such as qPCR, have been shown to have greater sensitivity than KK, especially at low prevalence...
January 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29345323/vernalization-can-regulate-flowering-time-through-microrna-mechanism-in-brassica-rapa
#5
Feiyi Huang, Xiaoting Wu, Xilin Hou, Shuaixu Shao, Tongkun Liu
Vernalization is an important process that regulates the floral transition in plants. MiRNAs are endogenous non-coding sRNA molecules that function in plant growth and development. Despite that miRNAs related to flowering have previously been characterized, miRNAs roles in response to vernalization in Pak-choi (Brassica rapa ssp. chinensis) has never been studied. Here, two sRNA libraries from Brassica rapa leaves (vernalized and non-vernalized plants) were constructed and sequenced. 208 known and 535 novel miRNAs were obtained, of which 20 known and 66 new miRNAs were significantly differentially expressed and considered as vernalization-related miRNAs...
January 18, 2018: Physiologia Plantarum
https://www.readbyqxmd.com/read/29345297/mir-135b-5p-promotes-gastric-cancer-progression-by-targeting-cmtm3
#6
Mingdong Lu, Yingpeng Huang, Weijian Sun, Pihong Li, Liyi Li, Leping Li
CKLF-like MARVEL transmembrane domain containing 3 (CMTM3) is considered to be a tumor suppressor gene in multiple types of malignancies. Previous studies have indicated that CMTM3 suppresses metastasis and epithelial-mesenchymal transition (EMT) in gastric cancer. However, its role in gastric cancer cell proliferation has rarely been discussed. Moreover, the regulatory mechanisms of CMTM3 in gastric cancer remain unclear. In this study, RT‑qPCR and IHC were used to assess the expression of CMTM3 and miR‑135b‑5p in gastric cancer tissues and cell lines...
February 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29344966/quorum-sensing-molecule-dihydroxy-2-3-pentanedione-and-its-analogs-as-regulators-of-epithelial-integrity
#7
S Elmanfi, X Ma, H O Sintim, E Könönen, S Syrjänen, U K Gursoy
BACKGROUND AND OBJECTIVE: Quorum-sensing molecules regulate the behavior of bacteria within biofilms and at the same time elicit an immune response in host tissues. Our aim was to investigate the regulatory role of dihydroxy-2,3-pentanedione (DPD), the precursor of universal autoinducer-2 (AI-2), and its analogs (ethyl-DPD, butyl-DPD and isobutyl-DPD) in the integrity of gingival epithelial cells. MATERIAL AND METHODS: Human gingival keratinocytes were incubated with four concentrations (10 μmol L-1 , 1 μmol L-1 , 100 nmol L-1 and 10 nmol L-1 ) of DPD and its analogs for 24 hours...
January 17, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29344960/association-of-genetic-variation-in-telomere-related-snp-and-telomerase-with-ventricular-arrhythmias-in-ischaemic-cardiomyopathy
#8
Vinit Sawhney, Scott Brouilette, Niall Campbell, Steven Coppen, Victoria Baker, Ross Hunter, Mehul Dhinoja, Atholl Johnston, Mark Earley, Simon Sporton, Ken Suzuki, Richard Schilling
BACKGROUND: Telomeres are known to provide genomic stability and telomere length has been associated with cardiovascular diseases. Moreover, a higher telomerase activity has been shown to be associated with ventricular arrhythmias (VA) in ischaemic cardiomyopathy. Increasing evidence suggests that genetic variation in key telomere genes has an impact on telomerase activity. Each copy of the minor allele of SNP rs12696304, at a locus including TERC (telomerase) has been associated with ∼ 75 bp reduction in mean telomere length likely mediated by an effect on TERC expression...
January 17, 2018: Pacing and Clinical Electrophysiology: PACE
https://www.readbyqxmd.com/read/29344870/cell-specific-rna-quantification-in-human-sn-da-neurons-from-heterogeneous-post-mortem-midbrain-samples-by-uv-laser-microdissection-and-rt-qpcr
#9
Johanna Duda, Michael Fauler, Jan Gründemann, Birgit Liss
Cell specificity of gene expression analysis is from particular relevance when the abundance of target cells is not homogeneous in the compared tissue samples, like it is the case, e.g., when comparing brain tissues from controls and in neurodegenerative disease states. While single-cell gene expression profiling is already a methodological challenge per se, it becomes even more prone to artifacts when analyzing individual cells from human post-mortem samples. Not only because human samples can never be matched as precisely as those from animal models, but also, because the RNA-quality that can be obtained from human samples usually displays a high range of variability...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344868/laser-capture-microdissection-for-the-analysis-of-rat-and-human-spinal-cord-ependyma-by-qpcr
#10
Daniel Garcia-Ovejero, Beatriz Paniagua-Torija, Angel Arevalo-Martin, Beatriz Navarro-Galve, Eduardo Molina-Holgado
In the last few decades many efforts have been dedicated to decipher the nature and regenerative potential of neurogenic niches and endogenous stem cells after damage of the central nervous system. In the spinal cord, it has been largely focused on the ependymal region, which hosts neural precursors/stem cells (NSC) in rodents but differs between species and ages. In the current chapter, we detail our protocol to study the gene expression profile of this region using fresh frozen blocks of rat and human post-mortem spinal cords...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344866/immuno-guided-laser-capture-microdissection-of-glial-cells-for-mrna-analysis
#11
Arnaud B Nicot, Justine Rambeau, Flora Guillot, Alexandra Garcia, David A Laplaud
Laser-capture microdissection (LCM) allows for retrieval of specific cell populations in situ. By combining immunofluorescent labeling with LCM, mRNAs can be probed by qRT-PCR for determining in situ gene expression during health and disease. This approach permits obtaining and analyzing histologically enriched cell populations in a tissue that can be hardly obtained from other methods such as white matter astrocytes from rodents or any individual cell population from archival human or rodent brain tissues...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344858/laser-capture-microdissection-as-a-tool-to-study-the-mucosal-immune-response-in-celiac-disease
#12
Giuseppe Iacomino, Vera Rotondi Aufiero, Pasquale Marena, Antonella Venezia, Riccardo Troncone, Salvatore Auricchio, Giuseppe Mazzarella
Laser capture microdissection (LCM) is a powerful tool for selection and isolation of single cells or compartments from complex primary tissues to perform molecular analyses. Celiac disease is a genetic autoimmune disorder where the ingestion of gluten leads to damage in the small intestine. Increased intraepithelial lymphocytes and the presence of the lamina propria inflammatory infiltrate of the duodenal mucosa is a common part of the disease. These cells promote inflammatory processes through the release of cytokines...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29344674/long-non-coding-rna-h1-promotes-cell-proliferation-and-invasion-by-acting-as-a-cerna-of-mir%C3%A2-138-and-releasing-ezh2-in-oral-squamous-cell-carcinoma
#13
Yonglong Hong, Haitao He, Wen Sui, Jingge Zhang, Shenfu Zhang, Dajiang Yang
Long non-coding RNAs (lncRNAs) have been shown to play pivotal roles in various types of human cancer, including oral squamous cell carcinoma (OSCC). However, the potential mechanisms of action of lncRNAs in OSCC remain to be fully elucidated. The aim of the present study was to further explore the potential mechanisms of action of lncRNAs in OSCC. We first analyzed Gene Expression Omnibus (GEO) datasets to investigate aberrantly expressed lncRNAs which may be involved in the development of OSCC. Reverse transcription‑quantitative PCR (RT‑qPCR) was performed to analyze the expression levels of lncRNA H19...
January 16, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29344673/golph2-a-gene-downstream-of-ras-signaling-promotes-the-progression-of-pancreatic-ductal-adenocarcinoma
#14
Juan Duan, Xiaoguang Li, Shan Huang, Yanhua Zeng, Yan He, Hekun Liu, Dexin Lin, Dongdong Lu, Min Zheng
Various studies have previously demonstrated that Golgi protein-73 (GOLPH2) is overexpressed in tumorigenesis, which has been observed in hepatocellular carcinoma and prostate cancer. However, the expression levels and specific functions of GOLPH2 in the progression of pancreatic cancer remain to be elucidated. The present study aimed to investigate the expression of GOLPH2 in pancreatic ductal adenocarcinoma (PDAC) tissues and examined the effects of GOLPH2 on the growth and migration of pancreatic cancer cells...
January 15, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29344659/degree-of-endplate-chondrocyte-degeneration-in-different-tension-regions-during-mechanical-stimulation
#15
Yongming Xu, Shujuan Xu, Zhi Gao, Liang Xiao, Fei Xiao, Hongguang Xu, Xiaoling Zhang
The aim of this study was to explore the degree of degeneration of endplate chondrocytes in different tension regions induced by intermittent cyclic mechanical tension (ICMT) in vitro. Rat endplate chondrocytes were harvested and treated with 10% ICMT for 8 h/day with a frequency of 0.5 Hz. A cartilage degeneration model was induced using an FX‑5000T cell strain‑loading system. The experiment was divided into the central region and the peripheral region, according to the contact area between the loading post and the six‑well flexible silicone rubber BioFlex plates...
January 16, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29344557/in-colonic-%C3%AF-0-rho0-cells-reduced-mitochondrial-function-mediates-transcriptomic-alterations-associated-with-cancer
#16
Harrison M Penrose, Sandra Heller, Chloe Cable, Hani Nakhoul, Nate Ungerleider, Melody Baddoo, Zachary F Pursell, Erik K Flemington, Susan E Crawford, Suzana D Savkovic
Background: Mitochondrial reprogramming has emerged as a hallmark of cancer pathobiology. Although it is believed this reprogramming is essential for cancer cells to thrive, how it supports cancer pathobiology is unclear. We previously generated colonic ρ0 (rho0) cells with reduced mitochondrial energy function and acquired their transcriptional signature. Here, we utilized a bioinformatics approach to identify their changes linked to cancer pathobiology. Methods: Human colon cancer HCT116 cells, control and ρ0, were used for qPCR...
November 2017: Oncoscience
https://www.readbyqxmd.com/read/29344347/epigenetic-alterations-in-tramp-mice-epigenome-dna-methylation-profiling-using-medip-seq
#17
Wenji Li, Ying Huang, Davit Sargsyan, Tin Oo Khor, Yue Guo, Limin Shu, Anne Yuqing Yang, Chengyue Zhang, Ximena Paredes-Gonzalez, Michael Verzi, Ronald P Hart, Ah-Ng Kong
Purpose: We investigated the genomic DNA methylation profile of prostate cancer in transgenic adenocarcinoma of the mouse prostate (TRAMP) cancer model and to analyze the crosstalk among targeted genes and the related functional pathways. Methods: Prostate DNA samples from 24-week-old TRAMP and C57BL/6 male mice were isolated. The DNA methylation profiles were analyzed by methylated DNA immunoprecipitation (MeDIP) followed by next-generation sequencing (MeDIP-seq)...
2018: Cell & Bioscience
https://www.readbyqxmd.com/read/29344236/alterations-in-expression-levels-of-genes-in-p53-related-pathways-determined-using-rna-seq-analysis-in-patients-with-breast-cancer-following-cik-therapy
#18
Zuowei Hu, Xiaoye Zhang, Hang Yang, Shuanglai Qin, Yaqi Liu, Wei Xiong, Bing Yuan, Liping Li, Weiqi Yao, Dongcheng Wu
The present study aimed at investigating the underlying molecular mechanisms for patients following cytokine-induced killer (CIK) therapy, particularly involving the alterations in p53-associated signaling pathways, to elucidate whether CIK therapy serves a function in cancer treatment. Samples of blood were collected from patients with breast cancer prior to and following CIK therapy. Two group samples were used for RNA sequencing (RNA-Seq) to determine the alterations in gene expression levels following CIK therapy and one for the quantitative polymerase chain reaction (qPCR), to analyze the reliability of RNA-Seq results...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344210/microrna-335-is-downregulated-in-papillary-thyroid-cancer-and-suppresses-cancer-cell-growth-migration-and-invasion-by-directly-targeting-zeb2
#19
Quan'e Kan, Yong Su, Huihui Yang
MicroRNAs (miRs) are a group of short, endogenous, non-protein-coding and single-stranded RNAs that regulate gene expression by binding to the 3'-untranslated region (3'UTR) of mRNAs, which results in their degradation or translational repression. The aim of the present study was to investigate the expression and function of miR-335 in human papillary thyroid cancer (PTC). Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was performed to quantify the relative miR-335 expression levels in PTC tissues and cell lines...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29344185/downregulation-of-mir-30a-is-associated-with-proliferation-and-invasion-via-targeting-mef2d-in-cervical-cancer
#20
Jing Zhao, Bo Li, Chuqiang Shu, Yun Ma, Yingping Gong
Accumulating studies have revealed that microRNAs serve crucial roles in cancer development and progression. MicroRNA-30a (miR-30a) has been implicated in various cancer types. However, the role of miR-30a in cervical cancer remains unclear. In the current study, a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assay revealed that miR-30a was significantly downregulated in cervical cancer tissues compared with adjacent normal tissues, and in the cervical cancer cell lines HeLa, SiHa and Ca-Ski compared with GH329 normal cervical epithelial cells...
December 2017: Oncology Letters
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