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https://www.readbyqxmd.com/read/29156776/uchl1-expression-and-localization-on-testicular-development-and-spermatogenesis-of-chinese-giant-salamanders
#1
Yuanxian Wang, Liqing Wang, Huihui Gao, Yao Gao, Changming Yang, Hong Ji, Wuzi Dong
Ubiquitin carboxyl-terminal hydrolase L1 (UCHL1), which is extensively expressed in vertebrates, is a deubiquitinating enzymes that inhibits the degradation of proteins by reversing ubiquitination modification. Herein, a 1087-bp sequence encoding UCHL1 was identified from the Chinese giant salamander (CGS; Andrias davidianus). The coding sequences (CDS) of UCHL1 encoded a putative poly peptide of 222 amino acids. The CGS UCHL1 isoforms were more related to their human and mouse counterparts. The phylogenic tree of vertebrate UCHL1 indicated that CGS UCHL1 has the closest relationship with human UCHL1 (up to 73...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156705/upregulation-of-cd11b-and-cd86-through-lsd1-inhibition-promotes-myeloid-differentiation-and-suppresses-cell-proliferation-in-human-monocytic-leukemia-cells
#2
Jianwu Fang, Haiyan Ying, Ting Mao, Yanjia Fang, Yuan Lu, He Wang, Irene Zang, Zhaofu Wang, Ying Lin, Mengxi Zhao, Xiao Luo, Zongyao Wang, Yan Zhang, Chao Zhang, Wei Xiao, Yan Wang, Wei Tan, Zhui Chen, Chris Lu, Peter Atadja, En Li, Kehao Zhao, Jianfeng Liu, Justin Gu
LSD1 (Lysine Specific Demethylase1)/KDM1A (Lysine Demethylase 1A), a flavin adenine dinucleotide (FAD)-dependent histone H3K4/K9 demethylase, sustains oncogenic potential of leukemia stem cells in primary human leukemia cells. However, the pro-differentiation and anti-proliferation effects of LSD1 inhibition in acute myeloid leukemia (AML) are not yet fully understood. Here, we report that small hairpin RNA (shRNA) mediated LSD1 inhibition causes a remarkable transcriptional activation of myeloid lineage marker genes (CD11b/ITGAM and CD86), reduction of cell proliferation and decrease of clonogenic ability of human AML cells...
October 17, 2017: Oncotarget
https://www.readbyqxmd.com/read/29156625/gene-based-pathogen-detection-can-we-use-qpcr-to-predict-the-outcome-of-diagnostic-metagenomics
#3
Sandra Christine Andersen, Mette Sofie Rousing Fachmann, Kristoffer Kiil, Eva Møller Nielsen, Jeffrey Hoorfar
In microbial food safety, molecular methods such as quantitative PCR (qPCR) and next-generation sequencing (NGS) of bacterial isolates can potentially be replaced by diagnostic shotgun metagenomics. However, the methods for pre-analytical sample preparation are often optimized for qPCR, and do not necessarily perform equally well for qPCR and sequencing. The present study investigates, through screening of methods, whether qPCR can be used as an indicator for the optimization of sample preparation for NGS-based shotgun metagenomics with a diagnostic focus...
November 20, 2017: Genes
https://www.readbyqxmd.com/read/29156604/takamatsu-h-comparison-of-minimal-residual-disease-detection-by-multiparameter-flow-cytometry-aso-qpcr-droplet-digital-pcr-and-deep-sequencing-in-patients-with-multiple-myeloma-who-underwent-autologous-stem-cell-transplantation-j-clin-med-2017-6-91
#4
https://www.readbyqxmd.com/read/29156242/optimization-of-pmaxx-pretreatment-to-distinguish-between-human-norovirus-with-intact-and-altered-capsids-in-shellfish-and-sewage-samples
#5
Walter Randazzo, Mohammad Khezri, Joanna Ollivier, Françoise S Le Guyader, Jesús Rodríguez-Díaz, Rosa Aznar, Gloria Sánchez
Shellfish contamination by human noroviruses (HuNoVs) is a serious health and economic problem. Recently an ISO procedure based on RT-qPCR for the quantitative detection of HuNoVs in shellfish has been issued, but these procedures cannot discriminate between inactivated and potentially infectious viruses. The aim of the present study was to optimize a pretreatment using PMAxx to better discriminate between intact and heat-treated HuNoVs in shellfish and sewage. To this end, the optimal conditions (30min incubation with 100μM of PMAxx and 0...
November 15, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29155860/chip-seq-analysis-identifies-p27-kip1-target-genes-involved-in-cell-adhesion-and-cell-signalling-in-mouse-embryonic-fibroblasts
#6
Atilla Biçer, Serena Orlando, Abul B M M K Islam, Edurne Gallastegui, Arnaud Besson, Rosa Aligué, Oriol Bachs, Maria Jesús Pujol
The protein p27Kip1 (p27), a member of the Cip-Kip family of cyclin-dependent kinase inhibitors, is involved in tumorigenesis and a correlation between reduced levels of this protein in human tumours and a worse prognosis has been established. Recent reports revealed that p27 also behaves as a transcriptional regulator. Thus, it has been postulated that the development of tumours with low amounts of p27 could be propitiated by deregulation of transcriptional programs under the control of p27. However, these programs still remain mostly unknown...
2017: PloS One
https://www.readbyqxmd.com/read/29155576/chronic-arsenic-exposure-increases-a%C3%AE-1-42-production-and-rage-expression-in-rat-brain
#7
Sandra Aurora Niño, Guadalupe Martel-Gallegos, Adriana Castro-Zavala, Benita Ortega-Berlanga, Juan Manuel Delgado, Hector Hernandez-Mendoza, E Romero-Guzman, Judith Rios-Lugo, Sergio Rosales-Mendoza, María Esther Jiménez-Capdeville, Sergio Zarazua
Chronic arsenic exposure during development is associated with alterations of chemical transmission and demyelination, which result in cognitive deficits and peripheral neuropathies. At cellular level, arsenic toxicity involves increased generation of reactive species that induce severe cellular alterations such as DNA fragmentation, apoptosis and lipid peroxidation. It has been proposed that arsenic-associated neurodegeneration could evolve to Alzheimer disease in later life.1,2 In this study, the effects of chronic exposure to inorganic arsenic (3 ppm by drinking water) in Wistar rats on the production and elimination of Amyloid-β (Aβ) were evaluated...
November 20, 2017: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29155298/mechanisms-of-l-alpha-lysophosphatidylinositol-induced-relaxation-in-human-pulmonary-arteries
#8
Olga Karpińska, Marta Baranowska-Kuczko, Barbara Malinowska, Monika Kloza, Magdalena Kusaczuk, Agnieszka Gęgotek, Paweł Golec, Irena Kasacka, Hanna Kozłowska
AIMS: l-Alpha-lysophosphatidylinositol (LPI) is an endogenous agonist of G protein-coupled receptor 55 (GPR55) which relaxes mesenteric arteries on activation. The aim of the present study was to determine the influence and underlying mechanisms of LPI-induced relaxation in human pulmonary arteries (hPAs). MAIN METHODS: Functional studies were performed in isolated hPAs using organ bath technique. The expression of GPR55 in hPAs and bronchioles was determined by real-time qPCR, Western blot analysis, and immunohistochemistry...
November 16, 2017: Life Sciences
https://www.readbyqxmd.com/read/29155258/insights-into-the-multifunctional-role-of-natural-killer-enhancing-factor-a-nkef-a-prx1-in-big-belly-seahorse-hippocampus-abdominalis-dna-protection-insulin-reduction-h2o2-scavenging-and-immune-modulation-activity
#9
G I Godahewa, N C N Perera, Jehee Lee
Natural killer enhancing factor A (NKEF-A), also known as peroxiredoxin 1 (Prx1), is a well-known antioxidant involved in innate immunity. Although NKEF-A/Prx1 has been studied in different fish species, the present study broadens the knowledge of NKEF-A gene in terms of molecular structure, function, and immune responses in fish species. Hippocampus abdominalis NKEF-A (HaNKEF-A) cDNA encoded a putative protein of 198 amino acids containing a thioredoxin_2 domain, VCP motifs, and three conserved cysteine residues including peroxidatic and resolving cysteines...
November 16, 2017: Gene
https://www.readbyqxmd.com/read/29155101/type-i-ifn-related-netosis-in-ataxia-telangiectasia-and-artemis-deficiency
#10
Ersin Gul, Esra Hazar Sayar, Bilgi Gungor, Fehime Kara Eroglu, Naz Surucu, Sevgi Keles, Sukru Nail Guner, Sıddıka Fındık, Esin Alpdündar, Ihsan Cihan Ayanoglu, Basak Kayaoglu, Busra Nur Geçkin, Hatice Asena Sanli, Tamer Kahraman, Cengiz Yakicier, Meltem Muftuoglu, Berna Oguz, Deniz Nazire Cagdas Ayvaz, Ihsan Gursel, Seza Ozen, Ismail Reisli, Mayda Gursel
BACKGROUND: Pathological inflammatory syndromes of unknown etiology are commonly observed in Ataxia telangiectasia (AT) and Artemis deficiency. Similar inflammatory manifestations also exist in STING-associated vasculopathy in infancy (SAVI) patients. OBJECTIVE: To test the hypothesis that the inflammation associated manifestations observed in AT and Artemis deficient patients stem from increased type I IFN signature leading to neutrophil mediated pathological damage...
November 15, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29155012/litopenaeus-vannamei-notch-affects-lipopolysaccharides-induced-reactive-oxygen-species
#11
Pei Ning, Zhihong Zheng, Jude Juventus Aweya, Defu Yao, Shengkang Li, Hongyu Ma, Fan Wang, Yueling Zhang
Notch signaling pathway was originally discovered in the development stage of drosophila but has recently been found to play essential roles in innate immunity. Most previous studies on Notch have focused on mammals, whereas, in this study, we employed the shrimp Litopenaeus vannamei as a model to study the functions of Notch in invertebrate innate immune system. Our results showed that LvNotch was highly expressed in hemocytes and could be strongly induced by lipopolysaccharides (LPS) injection. Small interfering RNA (siRNA)-mediated knockdown of LvNotch could significantly increase LPS induced L...
November 15, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29154214/ennoblement-corrosion-and-biofouling-in-brackish-seawater-comparison-between-six-stainless-steel-grades
#12
E Huttunen-Saarivirta, P Rajala, M Marja-Aho, J Maukonen, E Sohlberg, L Carpén
In this work, six common stainless steel grades were compared with respect to ennoblement characteristics, corrosion performance and tendency to biofouling in brackish sea water in a pilot-scale cooling water circuit. Two tests were performed, each employing three test materials, until differences between the materials were detected. Open circuit potential (OCP) was measured continuously in situ. Potentiodynamic polarization and electrochemical impedance spectroscopy (EIS) measurements were conducted before and after the tests...
November 5, 2017: Bioelectrochemistry
https://www.readbyqxmd.com/read/29154097/diversity-abundance-and-persistence-of-antibiotic-resistance-genes-in-various-types-of-animal-manure-following-industrial-composting
#13
Xun Qian, Jie Gu, Wei Sun, Xiao-Juan Wang, Jian-Qiang Su, Robert Stedfeld
Aerobic composting is used widely for animal manure recycling, and it may reduce the amount of antibiotic resistance genes (ARGs) that enter the environment. We sampled three types of animal (bovine, chicken, and pig) manure and the corresponding composts from 12 large-scale farms, and tested multiple ARGs and mobile genetic elements (MGEs) by high-throughput qPCR. A total of 109 ARGs were detected in the manure and compost samples, thereby demonstrating that both are important ARG reservoirs. The diversity and abundance of ARGs were significantly higher in chicken and pig manure than bovine manure, but industrial composting was more efficient at reducing the ARGs in chicken manure than pig and bovine manure...
November 13, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/29154080/changes-in-gene-expression-in-small-bowel-neuroendocrine-tumors-associated-with-progression-to-metastases
#14
Kendall J Keck, Patrick Breheny, Terry A Braun, Benjamin Darbro, Guiying Li, Joseph S Dillon, Andrew M Bellizzi, Thomas M O'Dorisio, James R Howe
BACKGROUND: Small bowel neuroendocrine tumors (SBNETs) present frequently with metastases, yet little is known about the molecular basis of this progression. This study sought to identify the serial differential expression of genes between normal small bowel, primary small bowel neuroendocrine tumors, and liver metastases. METHODS: RNA isolated from matched normal small bowel tissue, primary small bowel neuroendocrine tumors, and liver metastases in 12 patients was analyzed with whole transcriptome expression microarrays and RNA-Seq...
November 16, 2017: Surgery
https://www.readbyqxmd.com/read/29153776/francisella-noatunensis-subspecies-noatunensis-clpb-deletion-mutant-impairs-development-of-francisellosis-in-a-zebrafish-model
#15
Elisabeth O Lampe, Carl Zingmark, Julia I Tandberg, Ida Marie P Thrane, Espen Brudal, Anders Sjöstedt, Hanne C Winther-Larsen
BACKGROUND: Francisella noatunensis ssp. noatunensis (F.n.n.) is the causative agent of francisellosis in Atlantic cod and constitutes one of the main challenges for future aquaculture on this species. A facultative intracellular bacterium like F.n.n. exert an immunologic challenge against which live attenuated vaccines in general are most effective. Thus, we constructed a deletion in the F.n.n. clpB gene as ΔclpB mutants are among the most promising vaccine candidates in human pathogenic Francisella...
November 16, 2017: Vaccine
https://www.readbyqxmd.com/read/29153619/the-use-of-stool-specimens-reveals-helicobacter-pylori-strain-diversity-in-a-cohort-of-adolescents-and-their-family-members-in-a-developed-country
#16
Brendan Dolan, Lucy Burkitt-Gray, Stephen Shovelin, Billy Bourke, Brendan Drumm, Marion Rowland, Marguerite Clyne
Helicobacter pylori infection occurs within families but the transmission route is unknown. The use of stool specimens to genotype strains facilitates inclusion of complete families in transmission studies. Therefore, we aimed to use DNA from stools to analyze strain diversity in H. pylori infected families. We genotyped H. pylori strains using specific biprobe qPCR analysis of glmM, recA and hspA. Concentration of H. pylori organisms before DNA isolation enhanced subsequent DNA amplification. We isolated H...
November 13, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/29153474/impacts-of-environmental-factors-on-the-whole-microbial-communities-in-the-rhizosphere-of-a-metal-tolerant-plant-elsholtzia-haichowensis-sun
#17
Songqiang Deng, Tan Ke, Longtai Li, Shenwen Cai, Yuyue Zhou, Yue Liu, Limin Guo, Lanzhou Chen, Dayi Zhang
Rhizospheric microbes play important roles in plant growth and heavy metals (HMs) transformation, possessing great potential for the successful phytoremediation of environmental pollutants. In the present study, the rhizosphere of Elsholtzia haichowensis Sun was comprehensively studied to uncover the influence of environmental factors (EFs) on the whole microbial communities including bacteria, fungi and archaea, via quantitative polymerase chain reaction (qPCR) and high-throughput sequencing. By analyzing molecular ecological network and multivariate regression trees (MRT), we evaluated the distinct impacts of 37 EFs on soil microbial community...
November 15, 2017: Environmental Pollution
https://www.readbyqxmd.com/read/29153059/mid-to-late-gestational-changes-in-inflammatory-gene-expression-in-the-rat-placenta
#18
Kanchan Vaswani, Marloes Dekker Nitert, Hsiu-Wen Chan, Fatema B Almughlliq, Hassendrini N Peiris, Ryan J Wood-Bradley, James A Armitage, Gregory E Rice, Murray D Mitchell
BACKGROUND: The placenta plays an important role during pregnancy providing maternal blood supply from the uterus to the developing fetus. The structure and function of the placenta changes with gestation, as the fetus develops and its demands change. This study aims to elucidate changes in cytokine and chemokine gene expression throughout mid-to-late gestation in rat placenta. METHODS: Sprague Dawley rats were time-mated, and placentae were obtained from 6 pregnant dams at 4 different gestational periods: E14...
January 1, 2017: Reproductive Sciences
https://www.readbyqxmd.com/read/29152844/faecal-microbiota-in-dogs-with-multicentric-lymphoma
#19
A Gavazza, G Rossi, G Lubas, M Cerquetella, Y Minamoto, J S Suchodolski
Malignant lymphoma B-cell type is the most common canine haematopoietic malignancy. Changes in intestinal microbiota have been implicated in few types of cancer in humans. The aim of this prospective and case-control study was to determine differences in faecal microbiota between healthy control dogs and dogs with multicentric lymphoma. Twelve dogs affected by multicentric, B-cell, stage III-IV lymphoma, and 21 healthy dogs were enrolled in the study. For each dog, faecal samples were analysed by Illumina sequencing of 16S rRNA genes and quantitative PCR (qPCR) for selected bacterial groups...
November 20, 2017: Veterinary and Comparative Oncology
https://www.readbyqxmd.com/read/29152775/new-insights-into-diagnosis-and-therapeutic-options-for-proliferative-hepatoblastoma
#20
Katarzyna B Hooks, Jérôme Audoux, Helena Fazli, Sarah Lesjean, Tony Ernault, Nathalie-Dugot Senant, Thierry Leste-Lasserre, Martin Hagedorn, Benoit Rousseau, Coralie Danet, Sophie Branchereau, Laurence Brugières, Sophie Taque, Catherine Guettier, Monique Fabre, Anne Rullier, Marie-Annick Buendia, Thérèse Commes, Christophe F Grosset, Anne-Aurélie Raymond
Surgery and cisplatin-based treatment of hepatoblastoma (HB) currently guarantee the survival of 70-80% of patients. However, some important challenges remain in diagnosing high risk tumors and identifying relevant targetable pathways offering new therapeutic avenues. Previously, two molecular subclasses of hepatoblastoma tumors have been described, namely C1 and C2; C2 being the subgroup with the poorest prognosis, a more advanced tumor stage and the worst overall survival rate. An associated 16-gene signature to discriminate the two tumoral subgroups was proposed but it has not been transferred into clinical routine...
November 20, 2017: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
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