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Francisco Perez-Balderas, Sander I van Kasteren, Alaa A A Aljabali, Kim Wals, Sébastien Serres, Andrew Jefferson, Manuel Sarmiento Soto, Alexandre A Khrapitchev, James R Larkin, Claire Bristow, Seung Seo Lee, Guillaume Bort, Filippo De Simone, Sandra J Campbell, Robin P Choudhury, Daniel C Anthony, Nicola R Sibson, Benjamin G Davis
Ligand-conjugated microparticles of iron oxide (MPIO) have the potential to provide high sensitivity contrast for molecular magnetic resonance imaging (MRI). However, the accumulation and persistence of non-biodegradable micron-sized particles in liver and spleen precludes their clinical use and limits the translational potential of MPIO-based contrast agents. Here we show that ligand-targeted MPIO derived from multiple iron oxide nanoparticles may be coupled covalently through peptide linkers that are designed to be cleaved by intracellular macrophage proteases...
February 15, 2017: Nature Communications
Carles Justicia, Angélica Salas-Perdomo, Isabel Pérez-de-Puig, Lisette H Deddens, Geralda A F van Tilborg, Clara Castellví, Rick M Dijkhuizen, Ángel Chamorro, Anna M Planas
Hyperglycemia at stroke onset is associated with poor long-term clinical outcome in numerous studies. Hyperglycemia induces intracellular acidosis, lipid peroxidation, and peroxynitrite production resulting in the generation of oxidative and nitrosative stress in the ischemic tissue. Here, we studied the effects of acute hyperglycemia on in vivo intercellular adhesion molecule-1 (ICAM-1) expression, neutrophil recruitment, and brain damage after ischemia/reperfusion in mice and tested whether the natural antioxidant uric acid was protective...
December 15, 2016: Translational Stroke Research
Ding Gao, Xiu-Ping Lin, Zhi-Ping Zhang, Wei Li, Dong Men, Xian-En Zhang, Zong-Qiang Cui
UNLABELLED: Cellular delivery is an important concern for the efficiency of medicines and sensors for disease diagnoses and therapy. However, this task is quite challenging. Self-assembly virus capsid proteins might be developed as building blocks for multifunctional cellular delivery vehicles. In this work, we found that SV40 VP1 (Simian virus 40 major capsid protein) could function as a new cell-penetrating protein. The VP1 protein could carry foreign proteins into cells in a pentameric structure...
February 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
Fei Yan, Wei Yang, Xiang Li, Hongmei Liu, Xiang Nan, Lisi Xie, Dongliang Zhou, Guoxi Xie, Junru Wu, Bensheng Qiu, Xin Liu, Hairong Zheng
The goal of this study is to investigate the feasibility of using CD81- (Cluster of Differentiation 81 protein-) targeted microparticles of iron oxide (CD81-MPIO) for magnetic resonance imaging (MRI) of the murine atherosclerosis. CD81-MPIO and IgG- (Immunoglobulin G-) MPIO were prepared by covalently conjugating, respectively, with anti-CD81 monoclonal and IgG antibodies to the surface of the tosyl activated MPIO. The relevant binding capability of the MPIO was examined by incubating them with murine bEnd...
2015: BioMed Research International
N Patel, B A Duffy, A Badar, M F Lythgoe, E Årstad
Upregulation of cell adhesion molecules on endothelial cells is a hallmark of inflammation and an early feature of several neurological conditions. Here, we describe bimodal in vivo imaging of this inflammatory event in the brain using functionalized micron-sized particles of iron oxide. The particles were conjugated to anti-VCAM-1 antibodies and subsequently labeled with iodine-125. Radiolabeling of the antibody-coated particles was straightforward and proceeded in high radiochemical yields using commercially available iodination tubes...
August 19, 2015: Bioconjugate Chemistry
Yuanwei Yan, Sébastien Sart, Fabian Calixto Bejarano, Megan E Muroski, Geoffrey F Strouse, Samuel C Grant, Yan Li
Magnetic resonance imaging (MRI) provides an effective approach to track labeled pluripotent stem cell (PSC)-derived neural progenitor cells (NPCs) for neurological disorder treatments after cell labeling with a contrast agent, such as an iron oxide derivative. Cryopreservation of pre-labeled neural cells, especially in three-dimensional (3D) structure, can provide a uniform cell population and preserve the stem cell niche for the subsequent applications. In this study, the effects of cryopreservation on PSC-derived multicellular NPC aggregates labeled with micron-sized particles of iron oxide (MPIO) were investigated...
March 2015: Biotechnology Progress
Abby E Deans, Youssef Zaim Wadghiri, Orlando Aristizábal, Daniel H Turnbull
A prominent feature of the developing mammalian brain is the widespread migration of neural progenitor (NP) cells during embryogenesis. A striking example is provided by NP cells born in the ventral forebrain of mid-gestation stage mice, which subsequently migrate long distances to their final positions in the cortex and olfactory bulb. Previous studies have used two-dimensional histological methods, making it difficult to analyze three-dimensional (3D) migration patterns. Unlike histology, magnetic resonance microimaging (micro-MRI) is a non-destructive, quantitative and inherently 3D imaging method for analyzing mouse embryos...
July 1, 2015: NeuroImage
Dwayne R Roach, Wesley M Garrett, Glenn Welch, Thomas J Caperna, Neil C Talbot, Erik M Shapiro
Pig hepatocytes are an important investigational tool for optimizing hepatocyte transplantation schemes in both allogeneic and xenogeneic transplant scenarios. MRI can be used to serially monitor the transplanted cells, but only if the hepatocytes can be labeled with a magnetic particle. In this work, we describe culture conditions for magnetic cell labeling of cells from two different pig hepatocyte cell sources; primary pig hepatocytes (ppHEP) and stem cell-derived hepatocytes (PICM-19FF). The magnetic particle is a micron-sized iron oxide particle (MPIO) that has been extensively studied for magnetic cell labeling for MRI-based cell tracking...
2015: PloS One
Stavros Melemenidis, Andrew Jefferson, Neil Ruparelia, Asim M Akhtar, Jin Xie, Danny Allen, Alastair Hamilton, James R Larkin, Francisco Perez-Balderas, Sean C Smart, Ruth J Muschel, Xiaoyuan Chen, Nicola R Sibson, Robin P Choudhury
Angiogenesis is an essential component of tumour growth and, consequently, an important target both therapeutically and diagnostically. The cell adhesion molecule α(v)β(3) integrin is a specific marker of angiogenic vessels and the most prevalent vascular integrin that binds the amino acid sequence arginine-glycine-aspartic acid (RGD). Previous studies using RGD-targeted nanoparticles (20-50 nm diameter) of iron oxide (NPIO) for magnetic resonance imaging (MRI) of tumour angiogenesis, have identified a number of limitations, including non-specific extravasation, long blood half-life (reducing specific contrast) and low targeting valency...
2015: Theranostics
Ignace Mpio, Christine Cleaud, Walid Arkouche, Maurice Laville
INTRODUCTION: Undernutrition (UN) in chronic hemodialysis (CHD) is a recurrent complication and constitutes a major public health problem. This work aims to evaluate the effects of our nutritional strategy (NS) developed among malnourished patients (pts) in CHD. This is a prospective observational study conducted for 12 months (M) in a cohort of 132 pts in CHD including 49 women, mean age 66 ± 16 years and the dialysis vintage 72 ± 74 months. This NS is based on the action of a multidisciplinary team; it provides for moderate UN pts one first phase of an optimization of protein and energy intake in the daily meals; then depending on the clinical course a second phase of oral supplementation at home...
April 2015: Néphrologie & Thérapeutique
Anthony G Christodoulou, Yijen L Wu, T Kevin Hitchens, Chien Ho, Zhi-Pei Liang
Super-paramagnetic iron oxide (SPIO) particles can magnetically label immune cells in circulation; the accumulation of labeled cells can then be detected by magnetic resonance imaging (MRI). This has enormous potential for imaging inflammatory responses in the heart, but it has been difficult to do in vivo using conventional free-breathing, ungated cardiac imaging. Subspace imaging with temporal navigation and sparse sampling of (k, t)-space has previously been used to accelerate several cardiac imaging applications, conventionally alternating between acquiring navigator data and sparse data every other TR...
2014: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Sébastien Sart, Fabian Calixto Bejarano, Michelle A Baird, Yuanwei Yan, Jens T Rosenberg, Teng Ma, Samuel C Grant, Yan Li
BACKGROUND AIMS: Pluripotent stem cell (PSC)-derived neural progenitor cells (NPCs) represent an unlimited source for the treatment of various neurological disorders. NPCs are usually derived from PSCs through the formation of embryoid body (EB), an aggregate structure mimicking embryonic development. This study investigated the effect of labeling multicellular EB-NPC aggregates with micron-sized particles of iron oxide (MPIO) for cell tracking using magnetic resonance imaging (MRI). METHODS: Intact and dissociated EB-NPC aggregates were labeled with various concentrations of MPIOs (0, 2...
January 2015: Cytotherapy
Maxime Gauberti, Axel Montagne, Aurélien Quenault, Denis Vivien
Although the blood-brain barrier (BBB) was thought to protect the brain from the effects of the immune system, immune cells can nevertheless migrate from the blood to the brain, either as a cause or as a consequence of central nervous system (CNS) diseases, thus contributing to their evolution and outcome. Accordingly, as the interface between the CNS and the peripheral immune system, the BBB is critical during neuroinflammatory processes. In particular, endothelial cells are involved in the brain response to systemic or local inflammatory stimuli by regulating the cellular movement between the circulation and the brain parenchyma...
2014: Frontiers in Cellular Neuroscience
Rik P M Moonen, Pieternel van der Tol, Stefanie J C G Hectors, Lucas W E Starmans, Klaas Nicolay, Gustav J Strijkers
PURPOSE: To evaluate spin-lock MR for detecting superparamagnetic iron oxides and compare the detection sensitivity of quantitative T1ρ with T2 imaging. METHODS: In vitro experiments were performed to investigate the influence of iron oxide particle size and composition on T1ρ . These comprise T1ρ and T2 measurements (B0 = 1.41T) of agar (2%) with concentration ranges of three different iron oxide nanoparticles (IONs) (Sinerem, Resovist, and ION-Micelle) and microparticles of iron oxide (MPIO)...
December 2015: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
Sébastien Sart, Fabian Calixto Bejarano, Yuanwei Yan, Samuel C Grant, Yan Li
Due to the unlimited proliferation capacity and the unique differentiation ability of pluripotent stem cells (PSCs), including both embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), large numbers of PSC-derived cell products are in demand for applications in drug screening, disease modeling, and especially cell therapy. In stem cell-based therapy, tracking transplanted cells with magnetic resonance imaging (MRI) has emerged as a powerful technique to reveal cell survival and distribution...
2015: Methods in Molecular Biology
Dominik von Elverfeldt, Alexander Maier, Daniel Duerschmied, Moritz Braig, Thilo Witsch, Xiaowei Wang, Maximilian Mauler, Irene Neudorfer, Marius Menza, Marco Idzko, Andreas Zirlik, Timo Heidt, Peter Bronsert, Christoph Bode, Karlheinz Peter, Constantin von Zur Muhlen
BACKGROUND: Inflammation and myocardial necrosis play important roles in ischemia/reperfusion injury after coronary artery occlusion and recanalization. The detection of inflammatory activity and the extent of myocardial necrosis itself are of great clinical and prognostic interest. We developed a dual, noninvasive imaging approach using molecular magnetic resonance imaging in an in vivo mouse model of myocardial ischemia and reperfusion. METHODS AND RESULTS: Ischemia/reperfusion injury was induced in 10-week-old C57BL/6N mice by temporary ligation of the left anterior descending coronary artery...
August 19, 2014: Circulation
Erwin L A Blezer, Lisette H Deddens, Gijs Kooij, Joost Drexhage, Susanne M A van der Pol, Arie Reijerkerk, Rick M Dijkhuizen, Helga E de Vries
Upregulation of intercellular adhesion molecule 1 (ICAM-1) is an early event in lesion formation in multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE), an animal model of MS. Monitoring its expression may provide a biomarker for early disease activity and allow validation of anti-inflammatory interventions. Our objective was therefore to explore whether ICAM-1 expression can be visualized in vivo during EAE with magnetic resonance imaging (MRI) using micron-sized particles of iron oxide (MPIO), and to compare accumulation profiles of targeted and untargeted MPIO, and a gadolinium-containing agent...
March 2015: Contrast Media & Molecular Imaging
Dimitri Roose, Frederic Leroux, Nathalie De Vocht, Caroline Guglielmetti, Isabel Pintelon, Dirk Adriaensen, Peter Ponsaerts, Annemie Van der Linden, Sara Bals
In this study, the interaction between cells and micron-sized paramagnetic iron oxide (MPIO) particles was investigated by characterizing MPIO in their original state, and after cellular uptake in vitro as well as in vivo. Moreover, MPIO in the olfactory bulb were studied 9 months after injection. Using various imaging techniques, cell-MPIO interactions were investigated with increasing spatial resolution. Live cell confocal microscopy demonstrated that MPIO co-localize with lysosomes after in vitro cellular uptake...
November 2014: Contrast Media & Molecular Imaging
Andrew Jefferson, Neil Ruparelia, Robin P Choudhury
Targeting particles to sites of inflammation is of considerable interest for applications relating to molecular imaging and drug delivery. We and others have described micron-sized particles of iron oxide (MPIO) that can be directed using specific ligands (e.g. antibodies, peptides and oligosaccharides) to bind to mediators of vascular inflammation in vivo. Since leukocyte binding to these molecules can induce changes in the target cell, an outstanding question has been whether the binding of imaging particles to these mediators induces biologically significant changes in the endothelial cells, potentially initiating or propagating inflammation...
2013: Theranostics
Maxime Gauberti, Axel Montagne, Oscar A Marcos-Contreras, Audrey Le Béhot, Eric Maubert, Denis Vivien
BACKGROUND AND PURPOSE: Our aim was to assess the spatiotemporal evolution of the cerebrovascular inflammation occurring after ischemic and hemorrhagic strokes using a recently developed, fast, and ultra-sensitive molecular MRI method. METHODS: We first assessed longitudinally the cerebrovascular inflammation triggered by collagenase-induced hemorrhage and by permanent/transient middle cerebral artery occlusion in mice, using MRI after injection of microparticles of iron oxide targeted to vascular cell adhesion molecule-1 (MPIOs-αVCAM-1)...
July 2013: Stroke; a Journal of Cerebral Circulation
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