keyword
MENU ▼
Read by QxMD icon Read
search

Vcam-1

keyword
https://www.readbyqxmd.com/read/27924608/a-method-to-isolate-purify-and-characterize-human-periodontal-ligament-stem-cells
#1
Krzysztof Mrozik, Stan Gronthos, Songtao Shi, P Mark Bartold
Human periodontal ligament stem cells (PDLSCs) are a unique population of mesenchymal stem cells (MSCs) that demonstrate the capacity to generate cementum- and periodontal ligament-like structures in vivo. As such, PDLSCs represent a promising cell-based therapy in reconstructive dentistry for the treatment of periodontal disease. The present chapter describes two methods for isolating PDLSCs from human PDL tissue including traditional plastic adherence, and immunomagnetic selection based on the expression of MSC-associated surface markers STRO-1 antigen, CD146 (MUC-18), CD29 (Integrin β-1), CD44, and CD106 (VCAM-1)...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27920727/genetic-targeting-of-arginase-ii-in-mouse-prevents-renal-oxidative-stress-and-inflammation-in-diet-induced-obesity
#2
Ji Huang, Angana Rajapakse, Yuyan Xiong, Jean-Pierre Montani, François Verrey, Xiu-Fen Ming, Zhihong Yang
Obesity is associated with development and progression of chronic kidney disease (CKD). Recent evidence demonstrates that enhanced levels of the L-arginine:ureahydrolase, including the two isoenzymes arginase-I (Arg-I) and arginase-II (Arg-II) in vascular endothelial cells promote uncoupling of endothelial nitric oxide synthase (eNOS), leading to increased superoxide radical anion and decreased NO production thereby endothelial dysfunction. Arg-II but not Arg-I is abundantly expressed in kidney and the role of Arg-II in CKD is uncertain and controversial...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27919749/in-vitro-vla-4-blockade-results-in-an-impaired-nk-cell-mediated-immune-surveillance-against-melanoma
#3
Ilaria Gandoglia, Federico Ivaldi, Paolo Carrega, Eric Armentani, Guido Ferlazzo, Gianluigi Mancardi, Nicole Kerlero de Rosbo, Antonio Uccelli, Alice Laroni
Natalizumab (NTZ) is a monoclonal antibody targeting the α4β1 integrin (CD49d/CD29), very late antigen-4 (VLA-4), which is approved for treatment of relapsing-remitting multiple sclerosis (RR-MS). A possible association between NTZ treatment and a higher risk of melanoma is under debate. Natural Killer (NK) cells, which express VLA-4, represent an innate barrier limiting spreading of melanoma under steady state conditions. Indeed, because of their expression of activating receptors, they are very efficient in recognizing and killing melanoma cells without the need of a previous priming...
December 2, 2016: Immunology Letters
https://www.readbyqxmd.com/read/27919501/soluble-serum-vcam-1-whole-blood-mrna-expression-and-treatment-response-in-natalizumab-treated-multiple-sclerosis
#4
E R Petersen, H B Søndergaard, A B Oturai, Peh Jensen, P S Sorensen, F Sellebjerg, L Börnsen
BACKGROUND: Natalizumab reduces disease activity in multiple sclerosis (MS). Natalizumab binds to the very late antigen-4 and inhibits vascular cell adhesion molecule-1 (VCAM-1)-mediated transmigration of immune cells across the blood-brain-barrier. This is associated with decreased serum concentrations of soluble (s)VCAM-1 and an altered composition of immune cell-subsets in the blood. OBJECTIVE: We aimed to examine if sVCAM-1 serum concentrations and whole blood mRNA expression levels of immune activation biomarkers is associated with disease activity in natalizumab-treated MS-patients...
November 2016: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/27916547/role-of-several-cytokines-and-adhesion-molecules-in-the-diagnosis-and-prediction-of-survival-of-hepatocellular-carcinoma
#5
Raim Iliaz, Umit Akyuz, Didem Tekin, Murat Serilmez, Sami Evirgen, Bilger Cavus, Hilal Soydinc, Derya Duranyildiz, Cetin Karaca, Kadir Demir, Fatih Besisik, Sabahattin Kaymakoglu, Filiz Akyuz
BACKGROUND AND STUDY AIMS: There is still need for accurate markers for early diagnosis of hepatocellular carcinoma (HCC) and assessment of prognosis. The aim of this study is to investigate interleukin (IL)-32, IL-1 beta, IL-18, vascular cell adhesion molecule (VCAM)-1, and epithelial cell adhesion molecule (EpCAM) in the diagnosis and assessment of prognosis of HCC. PATIENTS AND METHODS: Fifty patients with HCC and 15 healthy volunteers were enroled into this prospective study...
December 1, 2016: Arab Journal of Gastroenterology: the Official Publication of the Pan-Arab Association of Gastroenterology
https://www.readbyqxmd.com/read/27915123/in-vitro-assessment-of-deferoxamine-on-mesenchymal-stromal-cells-from-tumor-and-bone-marrow
#6
Guoping Wang, Guobo Shen, Tao Yin
Deferoxamine (DFO), an iron chelator, is commonly used to remove excess iron from the body. DFO has also been demonstrated to have anti-tumor effect. However, there is no available report on the effect of deferoxamine on mesenchymal stromal cells (MSCs). In this study, we first isolated tumor-associated MSCs (TAMSCs) from EG-7 tumors, which were positive for CD29, CD44, CD73, CD90 and CD105. Ex vivo cultured stem cells derived from tumor and bone marrow compartment were exposed to DFO. We demonstrated that DFO had growth-arresting and apoptosis-inducing effect on TAMSCs and bone marrow MSCs (BMMSCs)...
November 23, 2016: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/27907087/prenylated-polyphenols-from-clusiaceae-and-calophyllaceae-with-immunomodulatory-activity-on-endothelial-cells
#7
Caroline Rouger, Sylvain Pagie, Séverine Derbré, Anne-Marie Le Ray, Pascal Richomme, Béatrice Charreau
Endothelial cells (ECs) are key players in inflammation and immune responses involved in numerous pathologies. Although attempts were experimentally undertaken to prevent and control EC activation, drug leads and probes still remain necessary. Natural products (NPs) from Clusiaceous and Calophyllaceous plants were previously reported as potential candidates to prevent endothelial dysfunction. The present study aimed to identify more precisely the molecular scaffolds that could limit EC activation. Here, 13 polyphenols belonging to 5 different chemical types of secondary metabolites (i...
2016: PloS One
https://www.readbyqxmd.com/read/27903586/microrna-125a-5p-alleviates-the-deleterious-effects-of-ox-ldl-on-multiple-functions-of-human-brain-microvessel-endothelial-cells
#8
Qunwen Pan, Xiaorong Liao, Hua Liu, Yan Wang, Yanfang Chen, Bin Zhao, Eric Lazartigues, Yi Yang, Xiaotang Ma
MicroRNA-125a-5p(miR-125a-5p)could participate in the pathogenesis of vascular diseases. In this study, we investigated the role of miR-125a-5p in oxidized low density lipoprotein (ox-LDL) induced functional changes in human brain microvessel endothelial cells (HBMEC). The reactive oxygen species (ROS) production, nitric oxide (NO) generation, senescence, apoptosis and functions of HBMEC were analyzed. For mechanism study, the epidermal growth factor receptor (EGFR)/ extracellular regulated protein kinases (ERK)/ p38 mitogen-activated protein kinase (p38 MAPK), and phosphatidylinositol-3-kinase (PI3K), serine/threonine kinase (Akt), endothelial nitric oxide synthase (eNOS), and p-Akt were analyzed...
November 30, 2016: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/27900989/negative-association-of-circulating-microrna-126-with-high-sensitive-c-reactive-protein-and-vascular-cell-adhesion-molecule-1-in-patients-with-coronary-artery-disease-following-percutaneous-coronary-intervention
#9
Jun-Nan Wang, You-You Yan, Zi-Yuan Guo, Ya-Juan Jiang, Lu-Lu Liu, Bin Liu
BACKGROUND: Percutaneous coronary intervention (PCI) causes endothelial damage, resulting in an inflammatory response with elevation of markers such as high-sensitive C-reactive protein (hs-CRP) and vascular cell adhesion molecule-1 (VCAM-1), which are associated with restenosis after PCI. Evidence suggests that microRNA-126 (miR-126) plays an important role in vascular inflammation, but its correlation with PCI-mediated inflammation has not been investigated. In this study, we investigated the effect of PCI on circulating miR-126 and inflammation markers such as hs-CRP and VCAM-1...
2016: Chinese Medical Journal
https://www.readbyqxmd.com/read/27898103/glyoxalase-1-knockdown-in-human-aortic-endothelial-cells-effect-on-the-proteome-and-endothelial-function-estimates
#10
Bernd Stratmann, Britta Engelbrecht, Britta C Espelage, Nadine Klusmeier, Janina Tiemann, Thomas Gawlowski, Yvonne Mattern, Martin Eisenacher, Helmut E Meyer, Naila Rabbani, Paul J Thornalley, Diethelm Tschoepe, Gereon Poschmann, Kai Stühler
Methylglyoxal (MG), an arginine-directed glycating agent, is implicated in diabetic late complications. MG is detoxified by glyoxalase 1 (GLO1) of the cytosolic glyoxalase system. The aim was to investigate the effects of MG accumulation by GLO1-knockdown under hyperglycaemic conditions in human aortic endothelial cells (HAECs) hypothesizing that the accumulation of MG accounts for the deleterious effects on vascular function. SiRNA-mediated knockdown of GLO1 was performed and MG concentrations were determined...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27897208/beta-1-integrin-ligation-and-tlr-ligation-enhance-gm-csf-induced-aldh1a2-expression-in-dendritic-cells-but-differentially-regulate-their-anti-inflammatory-properties
#11
Aya Yokota-Nakatsuma, Yoshiharu Ohoka, Hajime Takeuchi, Si-Young Song, Makoto Iwata
Retinoic acid (RA)-producing CD103(+) mature dendritic cells (DCs) in mesenteric lymph nodes (MLNs) play crucial roles in gut immunity. GM-CSF and RA contribute to the expression of the RA-producing enzyme ALDH1A2. However, additional signals appeared to be required for inducing ALDH1A2(high) mature DCs from immature DCs. We found here that TLR ligands (Ls) and immobilized E-cadherin could provide such signals in FLT3-L-generated bone marrow (BM)-derived DCs after treatment with GM-CSF and the RA receptor agonist Am80...
November 29, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27885839/the-association-of-plasma-mir-155-and-vcam-1-levels-with-coronary-collateral-circulation
#12
Junnan Wang, Youyou Yan, Dandan Song, Lulu Liu, Bin Liu
AIM: Inflammation plays an important role in development of coronary collateral circulation (CCC). The aim of this study is to determine whether the inflammation-related miRNA miR-155 and the inflammation marker VCAM-1 could be a biomarker for CCC. PATIENTS & METHODS: We measured levels of plasma VCAM-1 and miR-155 in patients with CCC according to Rentrop grade by ELISA or real-time polymerase chain reaction, respectively (n = 112). RESULTS: Plasma miR-155 was negatively correlated with VCAM-1 in the poor CCC group and with Rentrop grade in all patients with CCC...
November 25, 2016: Biomarkers in Medicine
https://www.readbyqxmd.com/read/27882144/effect-of-cyclosporin-a-intervention-on-the-immunological-mechanisms-of-coronary-heart-disease-and-restenosis
#13
Xuan Wang, Yue-Cheng Hu, Ru-Yan Zhang, Dong-Xia Jin, Yuan Jiang, He-Nan Zhang, Hong-Liang Cong
The present study aimed to investigate the effect of cyclosporin A (CSA) intervention on the immunological mechanisms underlying coronary heart disease (CHD) and restenosis (RS) in rabbits. A total of 48 rabbits were randomly divided into normal control (N), N + CSA, CHD model, CHD + CSA, RS model and RS + CSA groups. Rabbits in the respective groups received different treatments prior to sacrifice at the end of week 12. Iliac arteries were harvested from the rabbits for morphological analysis and to determine the mRNA and protein expression levels of cluster of differentiation (CD) 40/CD40 ligand (CD40L), CD134/CD134 ligand (CD134L) and inflammatory factors, including matrix metalloproteinase (MMP)-1, MMP-9, vascular cell adhesion protein (VCAM)-1, interleukin (IL)-6 and tumor necrosis factor (TNF)-α, by reverse transcription-quantitative polymerase chain reaction and immunohistochemical staining...
November 2016: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/27879252/high-density-lipoprotein-associated-apolipoprotein-m-limits-endothelial-inflammation-by-delivering-sphingosine-1-phosphate-to-the-sphingosine-1-phosphate-receptor-1
#14
Mario Ruiz, Cecilia Frej, Andreas Holmér, Li J Guo, Sinh Tran, Björn Dahlbäck
OBJECTIVE: Plasma high-density lipoproteins (HDL) are potent antiatherogenic and anti-inflammatory particles. However, HDL particles are highly heterogenic in composition, and different HDL-mediated functions can be ascribed to different subclasses of HDL. Only a small HDL population contains apolipoprotein M (ApoM), which is the main plasma carrier of the bioactive lipid mediator sphingosine-1-phosphate (S1P). Vascular inflammation is modulated by S1P, but both pro- and anti-inflammatory roles have been ascribed to S1P...
November 22, 2016: Arteriosclerosis, Thrombosis, and Vascular Biology
https://www.readbyqxmd.com/read/27878247/oridonin-inhibition-and-mir%C3%A2-200b%C3%A2-3p-zeb1-axis-in-human-pancreatic-cancer
#15
Zhifang Gui, Feng Luo, Yayang Yang, Can Shen, Shuquan Li, Jian Xu
The relationship among oridonin, miR-200b-3p and pancreatic cancer on epithelial-to-mesenchymal transition (EMT) was investigated for the molecular mechanism or signaling pathways on the migration in pancreatic cancer. BxPC-3 and PANC-1 cells were cultivated and the IC50 of oridonin in BxPC-3 and PANC-1 cells were obtained by the CCK-8 array. The expression of miR‑200b-3p was verified by using real-time PCR and its target gene was predicted. BxPC-3 and PANC-1 cells were treated with oridonin or transfected by miR-200b-3p, those cells were used for western blot assay, Transwell assay, ELISA, immunofluorescence staining, tumorigenesis assay in nude mice and immunohistochemical assay to verify the effects of oridonin or miR-200b-3p on pancreatic cancer...
November 18, 2016: International Journal of Oncology
https://www.readbyqxmd.com/read/27871463/pattern-of-adhesive-molecules-expression-in-placenta-of-non-complicated-art-pregnancies
#16
Ahmed A M Abdel-Hamid, Alaa El-Din L Firgany, Yaser Mesbah, Mona F M Soliman
OBJECTIVE: To investigate changes occurring in the morphometric parameters of chorionic villi and their vessels as well as in adhesive molecules expression in placenta of ART pregnancies. METHODS: Case-control study including a total of 52 placentas of non-complicated pregnancies of women delivered by spontaneous conception (SC) (n = 26) compared with those of ART (n = 26). Histological and morphometric assessment of fetal chorionic villi as well as the expression of various adhesive molecules (ICAM-1, VCAM-1 and PECAM-1) were performed in fetal plasma and placenta...
December 2016: Placenta
https://www.readbyqxmd.com/read/27870587/17%C3%AE-estradiol-enhances-vascular-endothelial-ets-1-mir-126-3p-expression-the-possible-mechanism-for-attenuation-of-atherosclerosis
#17
Ping Li, Jinzhi Wei, Xiaosa Li, Yang Cheng, Weiyu Chen, Yuhong Cui, Tommaso Simoncini, Zhengtian Gu, Jun Yang, Xiaodong Fu
CONTEXT: Endothelial microRNA 126 (miR-126) attenuates the development of atherosclerosis (AS). However, there is no evidence showing the role of miR-126 in estrogen's anti-atherogenic effects. OBJECTIVE: We hypothesized that 17β-estradiol (E2) modulates miR-126 expression, thus may improve endothelial function and retard AS development. DESIGN/SETTING/PARTICIPANTS: This was a prospective cohort study of 12 healthy regularly menstruating female volunteers aged 23-26 yr conducted at Guangzhou Medical University...
November 21, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27867386/immune-regulation-by-pericytes-modulating-innate-and-adaptive-immunity
#18
REVIEW
Rocío Navarro, Marta Compte, Luis Álvarez-Vallina, Laura Sanz
Pericytes (PC) are mural cells that surround endothelial cells in small blood vessels. PC have traditionally been credited with structural functions, being essential for vessel maturation and stabilization. However, an accumulating body of evidence suggests that PC also display immune properties. They can respond to a series of pro-inflammatory stimuli and are able to sense different types of danger due to their expression of functional pattern-recognition receptors, contributing to the onset of innate immune responses...
2016: Frontiers in Immunology
https://www.readbyqxmd.com/read/27867287/atherosclerosis-in-psoriatic-arthritis-a-multiparametric-analysis-using-imaging-technique-and-laboratory-markers-of-inflammation-and-vascular-function
#19
Nidhi Garg, Pawan Krishan, Ashit Syngle
Cardiovascular disease is one of the leading causes of death in psoriatic arthritis (PsA). Pathogenesis of accelerated atherosclerosis in PsA remains to be elucidated. Endothelial dysfunction (ED) often precedes manifesting atherosclerosis. This study aims to assess carotid intima-media thickness (CIMT), a marker of atherosclerosis in PsA, in context of markers of inflammation and vascular function. A cross-sectional study was performed in 18 PsA patients who were compared with 18 controls matched for age and sex...
December 2016: International Journal of Angiology: Official Publication of the International College of Angiology, Inc
https://www.readbyqxmd.com/read/27861612/clic1-inhibition-attenuates-vascular-inflammation-oxidative-stress-and-endothelial-injury
#20
Yingling Xu, Ji Zhu, Xiao Hu, Cui Wang, Dezhao Lu, Chenxue Gong, Jinhuan Yang, Lei Zong
Endothelial dysfunction, which includes endothelial oxidative damage and vascular inflammation, is a key initiating step in the pathogenesis of atherosclerosis (AS) and an independent risk factor for this disorder. Intracellular chloride channel 1 (CLIC1), a novel metamorphic protein, acts as a sensor of cell oxidation and is involved in inflammation. In this study, we hypothesize that CLIC1 plays an important role in AS. Apolipoprotein E-deficient mice were supplied with a normal diet or a high-fat and high-cholesterol diet for 8 weeks...
2016: PloS One
keyword
keyword
66150
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"