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Chromogenic media

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https://www.readbyqxmd.com/read/29074053/optimal-detection-of-carbapenemase-producing-enterobacteriaceae-from-rectal-samples-a-role-for-enrichment
#1
H Ciesielczuk, L M Phee, H Dolphin, M Wilks, B P Cherian, D W Wareham
BACKGROUND: Successful laboratory detection of carbapenemase-producing Enterobacteriaceae (CPE) in patient surveillance samples is a diagnostic challenge. In the absence of a gold standard for screening rectal swabs for CPE many phenotypic, genotypic, culture and non-culture based assays have been proposed for identifying these bacteria. AIM: To develop and optimise a CPE screening protocol capable of identifying all commonly encountered CPE, including those producing OXA-48-like carbapenemases...
October 23, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29064051/a-brief-review-on-fluorescent-copper-sensor-based-on-conjugated-organic-dyes
#2
REVIEW
Muhammad Saleem, Muhammad Rafiq, Muhammad Hanif, Muhammad Ashraf Shaheen, Sung-Yum Seo
The design and development of the fluorescence reporting molecules for the trace recognition of the metallic ions in the aqueous, mixed organic aqueous media, environmental specimen, living things e.g., body fluids, serum, urine etc is the evergreen research area. Among several methodologies utilized for these purposes, the fluorescent techniques have wonderful impact in the era of metal sensor development due to their acclamatory features of non-invasive detection process and appreciable sensitivity. The utilization of small organic molecules for the sensation of metallic ions through the fluorescent techniques harvested the wonderful results having some interesting features of chromogenic response on metal binding, robustness in the preparation and applicability to establish the metallic accumulation level inside the live cells via bioimaging studies...
October 24, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/29063353/high-prevalence-and-antimicrobial-resistance-of-meca-staphylococcus-aureus-in-dairy-cattle-sheep-and-goat-bulk-tank-milk-in-jordan
#3
Mohammad M Obaidat, Alaa E Bani Salman, Amira A Roess
The aim of this study was to determine the prevalence and antimicrobial resistance of mecA and mecC methicillin-resistant Staphylococcus aureus (MRSA) in cattle, sheep, and goat dairy farms in Jordan. Milk samples were collected from bulk tanks at 117 dairy farms (44 cattle, 47 sheep, and 26 goat dairy farms) in each region of the country. MRSA were isolated on mecA and mecC chromogenic media and confirmed by PCR. The confirmed isolates were tested for resistance toward 15 antimicrobials by the disc diffusion method...
October 23, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29026626/acute-diarrhoea-due-to-a-shiga-toxin-2e-producing-escherichia-coli-o8%C3%A2-%C3%A2-h19
#4
Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx2e-positive STEC of serotype O8 : H19...
June 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28984232/chromagar-col-apse-a-selective-bacterial-culture-medium-for-the-isolation-and-differentiation-of-colistin-resistant-gram-negative-pathogens
#5
Muhd Haziq F Abdul Momin, David C Bean, Rene S Hendriksen, Marisa Haenni, Lynette M Phee, David W Wareham
PURPOSE: A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin). METHODOLOGY: The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples...
October 6, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28978682/comparison-of-different-phenotypic-approaches-to-screen-and-detect-mecc-harboring-methicillin-resistant-staphylococcus-aureus
#6
André Kriegeskorte, Evgeny A Idelevich, Andreas Schlattmann, Franziska Layer, Birgit Strommenger, Olivier Denis, Gavin K Paterson, Mark A Holmes, Guido Werner, Karsten Becker
Similar to mecA, mecC confers resistance against beta-lactams, leading to the phenotype of a methicillin-resistant Staphylococcus aureus (MRSA). However, mecC-harboring MRSA pose special difficulties in their detection. The aim of this study was to assess and compare different phenotypic systems for screening, identification, and susceptibility testing of mecC-positive MRSA isolates. A well-characterized collection of mecC-positive S. aureus isolates (n = 111) was used for evaluation. Routinely used approaches were studied to determine their suitability to correctly identify mecC-harboring MRSA including three (semi-)automated antimicrobial susceptibility testing (AST) systems and five selective chromogenic agar plates...
October 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28951069/evaluation-of-single-versus-pooled-swab-cultures-for-detecting-mrsa-colonisation
#7
Irena Grmek-Kosnik, Dermota Urska, Ribic Helena, Storman Alenka, Petrovic Ziva, Zohar-Cretnik Tjasa
BACKGROUND: The cost and laboratory workload could be markedly reduced by processing nose, throat and skin swabs from one person in one culture broth (specimen pooling). The purpose of our study was to evaluate the sensitivity for MRSA detection and the growth rate of pooled swabs compared with individual processing using new chromogenic media (CROMagar MRSA and MRSA SMART) for identification of methicillin-resistant Staphylococcus aureus (MRSA). METHODS: In total, 417 swabs from 139 previous MRSA positive subjects (3 swabs per subject-nose, throat and skin) were submitted...
September 23, 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28903986/correction-for-perry-a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#8
John D Perry
No abstract text is available yet for this article.
October 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28882017/utility-of-rapid-microbiological-techniques-for-the-diagnosis-of-severe-infections
#9
REVIEW
E Cercenado
Rapid diagnostic microbiological techniques and antimicrobial susceptibility testing are necessary for early and adequate treatment. The utility of old (Gram stain, antigen detection, direct antimicrobial susceptibility testing, chromogenic media) and new techniques (molecular assays, MALDI-TOF) is summarized for the rapid diagnosis of bacteraemia and fungaemia, catheter-related bloodstream infections, pneumonia, meningitis, skin and soft-tissue infections, urinary tract infection, Clostridium difficile infection, viral infections, and tuberculosis...
September 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28809146/mycobacterium-talmoniae-sp-nov-a-slowly-growing-mycobacterium-isolated-from-human-respiratory-samples
#10
Rebecca M Davidson, Mary Ann DeGroote, Jamie L Marola, Sarah Buss, Victoria Jones, Michael R McNeil, Alison G Freifeld, L Elaine Epperson, Nabeeh A Hasan, Mary Jackson, Peter C Iwen, Max Salfinger, Michael Strong
A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28748285/toward-the-standardization-of-mycological-examination-of-sputum-samples-in-cystic-fibrosis-results-from-a-french-multicenter-prospective-study
#11
Noémie Coron, Marc Pihet, Emilie Fréalle, Yolande Lemeille, Claudine Pinel, Hervé Pelloux, Gilles Gargala, Loic Favennec, Isabelle Accoceberry, Isabelle Durand-Joly, Frédéric Dalle, Frédéric Huet, Annlyse Fanton, Amale Boldron, Guy-André Loeuille, Philippe Domblides, Bérengère Coltey, Isabelle Pin, Catherine Llerena, Françoise Troussier, Christine Person, Christophe Marguet, Nathalie Wizla, Caroline Thumerelle, Dominique Turck, Stéphanie Bui, Michael Fayon, Alain Duhamel, Anne Prévotat, Benoit Wallaert, Sylvie Leroy, Jean-Philippe Bouchara, Laurence Delhaes
Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: "MucoFong" program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media...
July 26, 2017: Mycopathologia
https://www.readbyqxmd.com/read/28663328/comparative-evaluation-of-six-chromogenic-media-for-presumptive-yeast-identification
#12
Alessandra Vecchione, Walter Florio, Francesco Celandroni, Simona Barnini, Antonella Lupetti, Emilia Ghelardi
AIMS: The present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. METHODS: We analysed 108 clinical isolates and reference strains belonging to eight different species: Candida albicans,Candida dubliniensis, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis,Candida lusitaniae and Trichosporon mucoides. RESULTS: C. albicans, C. tropicalis and C...
June 29, 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28659318/reliable-detection-of-group-b-streptococcus-in-the-clinical-laboratory
#13
REVIEW
Manuel Rosa-Fraile, Barbara Spellerberg
Group B streptococcus (GBS) is a leading cause of invasive neonatal infections and a significant pathogen in immunocompromised adults. Screening to detect GBS colonization in pregnant women determines the need for antibiotic prophylaxis in that pregnancy. Efficient determination of the GBS colonization status of pregnant women is crucial. Methods that maximize the probability of GBS recovery are needed. The availability of technologies such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular techniques, and chromogenic culture media, including Granada-type media, have changed the scenario for GBS detection and identification...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28604906/a-selective-emissive-chromogenic-and-fluorogenic-seleno-coumarin-probe-for-cu-2-detection-in-aprotic-media
#14
A C Gonçalves, J Luis Capelo, C Lodeiro, A A Dos Santos
A new selenium containing coumarin (compound 7) was designed and synthesized from the amide linkage between coumarin-519 (6) and 2-(butylselanyl)ethanamine (5). The molecular structure of 7 was accurately characterized, and its photophysical properties in acetonitrile, ethanol and chloroform solutions were studied by absorption, stationary and time-resolved fluorescence spectroscopies. Changes in the solvent polarity affected the Stokes shift, quantum yields and lifetime of the excited states. The spectroscopic behavior of compound 7 was evaluated in the presence of different monovalent, divalent and trivalent metallic cations (Na(+), K(+), Ca(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), Pb(2+), Hg(2+), Hg(+), Ag(+), Al(3+), Fe(3+), Ga(3+) and Cr(3+)) in acetonitrile solution...
July 1, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28594779/clinical-validation-of-a-test-for-the-diagnosis-of-vaginitis
#15
MULTICENTER STUDY
Charlotte A Gaydos, Sajo Beqaj, Jane R Schwebke, Joel Lebed, Bonnie Smith, Thomas E Davis, Kenneth H Fife, Paul Nyirjesy, Timothy Spurrell, Dorothy Furgerson, Jenell Coleman, Sonia Paradis, Charles K Cooper
OBJECTIVE: Vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. A new molecular test assays the vaginal microbiome and organisms that cause three common infections. The objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. The primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of vaginitis and compare clinician-collected with self-collected swabs...
July 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28559163/a-cost-saving-strategy-for-processing-isolated-uropathogens-in-community-acquired-urinary-tract-infections
#16
COMPARATIVE STUDY
Juan Manuel Sánchez-Calvo, José Luís de Francisco, Eva Torres-Martos, Juan Carlos Alados Arboledas, Maria Dolores López Prieto
Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total)...
August 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28549606/variable-performance-of-four-commercial-chromogenic-media-for-detection-of-methicillin-resistant-staphylococcus-aureus-isolates-harbouring-mecc
#17
Céline Dupieux, Camille Kolenda, Anders Rhod Larsen, Bruno Pichon, Mark Holmes, Michèle Bes, Christopher Teale, Elizabeth Dickson, Robert Hill, Robert Skov, Angela Kearns, Frédéric Laurent
In this study, the performances of four methicillin-resistant Staphylococcus aureus (MRSA) chromogenic screening media were compared for detecting mecC-positive MRSA. Using 111 clinical isolates representative of the various mecC-MRSA clones in Europe, chromID(®) MRSA (bioMérieux) and Brilliance(TM) MRSA 2 (Oxoid Ltd.) showed higher sensitivity (99.1% and 97.3%, respectively) than BBL(TM) CHROMagar(®) MRSA II (BD Diagnostics) and MRSASelect(TM) (Bio-Rad) (79.3% and 63.1%, respectively) (P <0.0001). These findings have important implications for effective public health diagnostics and epidemiological surveillance of MRSA...
May 23, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28511380/fungal-profile-of-vulvovaginal-candidiasis-in-a-tertiary-care-hospital
#18
Krishnapriya Kalaiarasan, Rakesh Singh, Latha Chaturvedula
INTRODUCTION: Vulvovaginal Candidiasis (VVC) is a common medical health problem of adult women. It is most commonly caused by Candida albicans. But there is a change in fungal profile. Sabouraud's Dextrose Agar (SDA) is the most common culture medium used where mixed fungal infection may be missed. It can be detected easily by using chromogenic culture medium. AIM: To know the fungal profile of vulvovaginal candidiasis using Candida CHROMagar and antifungal susceptibility pattern in patients attending tertiary care hospital...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28505508/phosphatase-activities-of-a-microepiphytic-community-during-a-bloom-of-ostreopsis-cf-ovata-in-the-northern-adriatic-sea
#19
Stefano Accoroni, Cecilia Totti, Emanuela Razza, Roberta Congestri, Alessandra Campanelli, Mauro Marini, Neil Thomas William Ellwood
It is becoming more apparent that increased organic nutrient loads deriving from anthropogenic activities and natural processes frequently cause the eutrophication of coastal waters. Concurrently, an increasing number of phototrophs have been shown to make use of organic nutrients, mainly through indirect studies of surface enzyme activities or through direct studies of growth in media containing organic-only nutrients. The potential utilization of dissolved organic phosphorus (DOP) by microepiphytic-mats associated with frequently problematic, toxic dinoflagellate Ostreopsis cf...
September 1, 2017: Water Research
https://www.readbyqxmd.com/read/28467187/evaluation-of-two-standard-and-two-chromogenic-selective-media-for-optimal-growth-and-enumeration-of-isolates-of-16-unique-bacillus-species
#20
M Shahjahan Kabir, Ying-Hsin Hsieh, Steven Simpson, Khalil Kerdahi, Irshad M Sulaiman
The genus Bacillus is a group of gram-positive endospore-forming bacteria that can cause food poisoning and diarrheal illness in humans. A wide range of food products have been linked to foodborne outbreaks associated with these opportunistic pathogens. The U.S. Food and Drug Administration recommends (in their Bacteriological Analytical Manual) the use of Bacara or mannitol egg yolk polymyxin (MYP) agar plates and the most-probable-number (MPN) method for enumeration and confirmation of Bacillus cereus and related species isolated from foods, sporadic cases, outbreaks, and routine environmental surveillance samples...
June 2017: Journal of Food Protection
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