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Chromogenic media

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https://www.readbyqxmd.com/read/29679603/chromogenic-culture-media-or-rapid-immunochromatographic-test-which-is-better-for-detecting-klebsiella-pneumoniae-that-produce-oxa-48-and-can-they-be-used-in-blood-and-urine-specimens
#1
Ozlem Genc, Evrim Aksu
Our goal was to compare a rapid test (OXA-48K-SeT) and four different chromogenic media (CHROMagar KPC, CHROMagar mSuperCARBA, ChromID Carba and ChromID OXA-48) for the detection of OXA-48 producing Klebsiella pneumoniae isolates and spiked urine/blood samples with these bacteria. In total 100 K.pneumoniae isolates, including 60 OXA-48 positive, 15 other carbapenemase producing, 15 Extended spectrum betalactamases (ESBL) positive and 10 carbapenem sensitive K.pneumoniae were included in the study. After all samples were inoculated into all chromogenic media, temocillin discs were placed onto the media...
May 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29576592/comparison-of-chromogenic-selective-media-for-the-detection-of-cronobacter-spp-enterobacter-sakazakii
#2
Hajime Teramura, Noriko Fukuda, Yumiko Okada, Hirokazu Ogihara
 The four types of chromogenic selective media that are commercially available in Japan were compared for establishing a Japanese standard method for detecting Cronobacter spp. based on ISO/TS 22964:2006. When assessed using 9 standard Cronobacter spp. strains and 29 non-Cronobacter strains, Enterobacter sakazakii isolation agar, ChromocultTM Enterobacter sakazakii agar, CHROMagarTM E. sakazakii, and XM-sakazakii agar demonstrated excellent inclusivity and exclusivity. Using the ISO/TS 22964:2006 method, the recovered numbers of 38 Cronobacter spp...
2018: Biocontrol Science
https://www.readbyqxmd.com/read/29383574/a-fatal-case-of-candida-auris-and-candida-tropicalis-candidemia-in-neutropenic-patient
#3
Ratna Mohd Tap, Teck Choon Lim, Nur Amalina Kamarudin, Stephanie Jane Ginsapu, Mohd Fuat Abd Razak, Norazah Ahmad, Fairuz Amran
We report a fatal case of Candida auris that was involved in mixed candidemia with Candida tropicalis, isolated from the blood of a neutropenic patient. Identification of both isolates was confirmed by amplification and sequencing of internal transcribed spacer and D1/D2 domain of large subunit in rRNA gene. Antifungal susceptibility test by E-test method revealed that C. auris was resistant to amphotericin B, anidulafungin, caspofungin, fluconazole, itraconazole and voriconazole. On the other hand, C. tropicalis was sensitive to all antifungal tested...
January 30, 2018: Mycopathologia
https://www.readbyqxmd.com/read/29274486/tellurite-resistance-profiles-and-performance-of-different-chromogenic-agars-for-detection-of-non-o157-shiga-toxin-producing-escherichia-coli
#4
Ruyue Fan, Xiangning Bai, Shanshan Fu, Yanmei Xu, Hui Sun, Hong Wang, Yanwen Xiong
Shiga toxin-producing Escherichia coli (STEC) are globally important food-borne pathogens. The isolation of non-O157 STEC is a significant public health challenge due to the dramatic diversity of their phenotypes and genotypes. In the present study, 476 non-O157 STEC strains representing 95 different O-serogroups were used to evaluate tellurite resistance and the performance of 12 different chromogenic agars. Of 476 strains, only 108 (22.7%) strains showed the minimal inhibitory concentration (MIC) values for potassium tellurite being higher than 4μg/ml, and 96 (20...
February 2, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/29260855/cellulose-based-sensor-containing-phenanthroline-for-the-highly-selective-and-rapid-detection-of-fe-2-ions-with-naked-eye-and-fluorescent-dual-modes
#5
Haq Nawaz, Weiguo Tian, Jinming Zhang, Ruonan Jia, Zhangyan Chen, Jun Zhang
Iron ions play a vital role in many biological processes, and their concentrations are responsible for human health. Therefore, it is essential to detect the concentration of iron ions by a rapid, accurate, highly selective, and practical method. Herein, we have synthesized a cellulose-based fluorescent sensor (Phen-MDI-CA) for the highly selective and rapid detection of Fe2+ ions via chemically bonding 1,10-phenanthroline-5-amine (Phen) onto cellulose acetate (CA) using 4,4'-methylene diphenyl diisocyanate (MDI) as a cross-linker...
January 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29174735/comparative-study-of-3-carbapenem-hydrolysis-methods-for-the-confirmation-of-carbapenemase-production-in-enterobacteriaceae
#6
COMPARATIVE STUDY
Ohad Shalom, Amos Adler
The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the β-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the β-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%)...
February 2018: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/29074053/optimal-detection-of-carbapenemase-producing-enterobacteriaceae-from-rectal-samples-a-role-for-enrichment
#7
H Ciesielczuk, L M Phee, H Dolphin, M Wilks, B P Cherian, D W Wareham
BACKGROUND: Successful laboratory detection of carbapenemase-producing Enterobacteriaceae (CPE) in patient surveillance samples is a diagnostic challenge. In the absence of a reference standard for screening rectal swabs for CPE, many phenotypic, genotypic, culture- and non-culture-based assays have been proposed for identifying these bacteria. AIM: To develop and optimize a CPE screening protocol capable of identifying all frequently encountered CPE, including those producing OXA-48-like carbapenemases...
March 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/29064051/a-brief-review-on-fluorescent-copper-sensor-based-on-conjugated-organic-dyes
#8
REVIEW
Muhammad Saleem, Muhammad Rafiq, Muhammad Hanif, Muhammad Ashraf Shaheen, Sung-Yum Seo
The design and development of the fluorescence reporting molecules for the trace recognition of the metallic ions in the aqueous, mixed organic aqueous media, environmental specimen, living things e.g., body fluids, serum, urine etc is the evergreen research area. Among several methodologies utilized for these purposes, the fluorescent techniques have wonderful impact in the era of metal sensor development due to their acclamatory features of non-invasive detection process and appreciable sensitivity. The utilization of small organic molecules for the sensation of metallic ions through the fluorescent techniques harvested the wonderful results having some interesting features of chromogenic response on metal binding, robustness in the preparation and applicability to establish the metallic accumulation level inside the live cells via bioimaging studies...
January 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29063353/high-prevalence-and-antimicrobial-resistance-of-meca-staphylococcus-aureus-in-dairy-cattle-sheep-and-goat-bulk-tank-milk-in-jordan
#9
Mohammad M Obaidat, Alaa E Bani Salman, Amira A Roess
The aim of this study was to determine the prevalence and antimicrobial resistance of mecA and mecC methicillin-resistant Staphylococcus aureus (MRSA) in cattle, sheep, and goat dairy farms in Jordan. Milk samples were collected from bulk tanks at 117 dairy farms (44 cattle, 47 sheep, and 26 goat dairy farms) in each region of the country. MRSA were isolated on mecA and mecC chromogenic media and confirmed by PCR. The confirmed isolates were tested for resistance toward 15 antimicrobials by the disc diffusion method...
February 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29026626/acute-diarrhoea-due-to-a-shiga-toxin-2e-producing-escherichia-coli-o8-h19
#10
Angela Saupe, Birgit Edel, Wolfgang Pfister, Bettina Löffler, Ralf Ehricht, Jürgen Rödel
Introduction. Identification of non-O157 Shiga-toxin-producing Escherichia coli (STEC) infections may be underestimated in microbiological diagnosis. Case presentation. A 58-year-old woman developed diarrhoea with watery and subsequently mucous stool. Initial multiplex PCR testing revealed a positive result for stx2 . Culture isolation of a STEC was successful only after repeated inoculation of chromogenic E. coli media. Molecular characterization was performed and identified the isolate as stx 2e -positive STEC of serotype O8 : H19...
June 2017: JMM Case Reports
https://www.readbyqxmd.com/read/28984232/chromagar-col-apse-a-selective-bacterial-culture-medium-for-the-isolation-and-differentiation-of-colistin-resistant-gram-negative-pathogens
#11
Muhd Haziq F Abdul Momin, David C Bean, Rene S Hendriksen, Marisa Haenni, Lynette M Phee, David W Wareham
PURPOSE: A selective chromogenic culture medium for the laboratory isolation and differentiation of colistin resistant Acinetobacter, Pseudomonas, Stenotrophomonas and Enterobacteriaceae spp. (CHROMagar COL-APSE) was developed, evaluated and compared to an existing selective bacterial culture medium (SuperPolymyxin). METHODOLOGY: The medium was challenged with 84 isolates, including polymyxin B (POL B)-susceptible and -resistant type strains and colistin (COL)-resistant organisms recovered from human and animal samples...
November 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28978682/comparison-of-different-phenotypic-approaches-to-screen-and-detect-mecc-harboring-methicillin-resistant-staphylococcus-aureus
#12
André Kriegeskorte, Evgeny A Idelevich, Andreas Schlattmann, Franziska Layer, Birgit Strommenger, Olivier Denis, Gavin K Paterson, Mark A Holmes, Guido Werner, Karsten Becker
Similar to mecA , mecC confers resistance against beta-lactams, leading to the phenotype of methicillin-resistant Staphylococcus aureus (MRSA). However, mecC -harboring MRSA strains pose special difficulties in their detection. The aim of this study was to assess and compare different phenotypic systems for screening, identification, and susceptibility testing of mecC -positive MRSA isolates. A well-characterized collection of mecC -positive S. aureus isolates ( n = 111) was used for evaluation. Routinely used approaches were studied to determine their suitability to correctly identify mecC -harboring MRSA, including three (semi)automated antimicrobial susceptibility testing (AST) systems and five selective chromogenic agar plates...
January 2018: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28951069/evaluation-of-single-vs-pooled-swab-cultures-for-detecting-mrsa-colonization
#13
I Grmek-Kosnik, U Dermota, H Ribic, A Storman, Z Petrovic, T Zohar-Cretnik
BACKGROUND: The costs and laboratory workload arising from meticillin-resistant Staphylococcus aureus (MRSA) screening could be reduced markedly by processing nose, throat and skin swabs from one person in a single culture broth (specimen pooling). The purpose of this study was to evaluate the sensitivities and times for MRSA detection using a variety of approaches to processing of individual and pooled swabs. METHODS: Four hundred and seventeen swabs from 139 subjects with a history of MRSA colonization (three swabs per subject - nose, throat and skin) were submitted...
February 2018: Journal of Hospital Infection
https://www.readbyqxmd.com/read/28903986/correction-for-perry-a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#14
John D Perry
No abstract text is available yet for this article.
October 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28882017/utility-of-rapid-microbiological-techniques-for-the-diagnosis-of-severe-infections
#15
REVIEW
E Cercenado
Rapid diagnostic microbiological techniques and antimicrobial susceptibility testing are necessary for early and adequate treatment. The utility of old (Gram stain, antigen detection, direct antimicrobial susceptibility testing, chromogenic media) and new techniques (molecular assays, MALDI-TOF) is summarized for the rapid diagnosis of bacteraemia and fungaemia, catheter-related bloodstream infections, pneumonia, meningitis, skin and soft-tissue infections, urinary tract infection, Clostridium difficile infection, viral infections, and tuberculosis...
September 2017: Revista Española de Quimioterapia: Publicación Oficial de la Sociedad Española de Quimioterapia
https://www.readbyqxmd.com/read/28809146/mycobacterium-talmoniae-sp-nov-a-slowly-growing-mycobacterium-isolated-from-human-respiratory-samples
#16
Rebecca M Davidson, Mary Ann DeGroote, Jamie L Marola, Sarah Buss, Victoria Jones, Michael R McNeil, Alison G Freifeld, L Elaine Epperson, Nabeeh A Hasan, Mary Jackson, Peter C Iwen, Max Salfinger, Michael Strong
A novel slowly growing, non-chromogenic species of the class Actinobacteria was isolated from a human respiratory sample in Nebraska, USA, in 2012. Analysis of the internal transcribed spacer sequence supported placement into the genus Mycobacterium with high sequence similarity to a previously undescribed strain isolated from a patient respiratory sample from Oregon, USA, held in a collection in Colorado, USA, in 2000. The two isolates were subjected to phenotypic testing and whole genome sequencing and found to be indistinguishable...
August 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28748285/toward-the-standardization-of-mycological-examination-of-sputum-samples-in-cystic-fibrosis-results-from-a-french-multicenter-prospective-study
#17
Noémie Coron, Marc Pihet, Emilie Fréalle, Yolande Lemeille, Claudine Pinel, Hervé Pelloux, Gilles Gargala, Loic Favennec, Isabelle Accoceberry, Isabelle Durand-Joly, Frédéric Dalle, Frédéric Huet, Annlyse Fanton, Amale Boldron, Guy-André Loeuille, Philippe Domblides, Bérengère Coltey, Isabelle Pin, Catherine Llerena, Françoise Troussier, Christine Person, Christophe Marguet, Nathalie Wizla, Caroline Thumerelle, Dominique Turck, Stéphanie Bui, Michael Fayon, Alain Duhamel, Anne Prévotat, Benoit Wallaert, Sylvie Leroy, Jean-Philippe Bouchara, Laurence Delhaes
Fungal respiratory colonization of cystic fibrosis (CF) patients emerges as a new concern; however, the heterogeneity of mycological protocols limits investigations. We first aimed at setting up an efficient standardized protocol for mycological analysis of CF sputa that was assessed during a prospective, multicenter study: "MucoFong" program (PHRC-06/1902). Sputa from 243 CF patients from seven centers in France were collected over a 15-month period and submitted to a standardized protocol based on 6 semi-selective media...
February 2018: Mycopathologia
https://www.readbyqxmd.com/read/28663328/comparative-evaluation-of-six-chromogenic-media-for-presumptive-yeast-identification
#18
COMPARATIVE STUDY
Alessandra Vecchione, Walter Florio, Francesco Celandroni, Simona Barnini, Antonella Lupetti, Emilia Ghelardi
AIMS: The present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. METHODS: We analysed 108 clinical isolates and reference strains belonging to eight different species: Candida albicans , Candida dubliniensis , Candida tropicalis , Candida krusei , Candida glabrata , Candida parapsilosis , Candida lusitaniae and Trichosporon mucoides . RESULTS: C. albicans , C. tropicalis and C...
December 2017: Journal of Clinical Pathology
https://www.readbyqxmd.com/read/28659318/reliable-detection-of-group-b-streptococcus-in-the-clinical-laboratory
#19
REVIEW
Manuel Rosa-Fraile, Barbara Spellerberg
Group B streptococcus (GBS) is a leading cause of invasive neonatal infections and a significant pathogen in immunocompromised adults. Screening to detect GBS colonization in pregnant women determines the need for antibiotic prophylaxis in that pregnancy. Efficient determination of the GBS colonization status of pregnant women is crucial. Methods that maximize the probability of GBS recovery are needed. The availability of technologies such as matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), molecular techniques, and chromogenic culture media, including Granada-type media, have changed the scenario for GBS detection and identification...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28604906/a-selective-emissive-chromogenic-and-fluorogenic-seleno-coumarin-probe-for-cu-2-detection-in-aprotic-media
#20
A C Gonçalves, J Luis Capelo, C Lodeiro, A A Dos Santos
A new selenium containing coumarin (compound 7) was designed and synthesized from the amide linkage between coumarin-519 (6) and 2-(butylselanyl)ethanamine (5). The molecular structure of 7 was accurately characterized, and its photophysical properties in acetonitrile, ethanol and chloroform solutions were studied by absorption, stationary and time-resolved fluorescence spectroscopies. Changes in the solvent polarity affected the Stokes shift, quantum yields and lifetime of the excited states. The spectroscopic behavior of compound 7 was evaluated in the presence of different monovalent, divalent and trivalent metallic cations (Na+ , K+ , Ca2+ , Co2+ , Ni2+ , Cu2+ , Zn2+ , Cd2+ , Pb2+ , Hg2+ , Hg+ , Ag+ , Al3+ , Fe3+ , Ga3+ and Cr3+ ) in acetonitrile solution...
July 1, 2017: Photochemical & Photobiological Sciences
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