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Chromogenic media

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https://www.readbyqxmd.com/read/28267570/identification-of-microorganisms-grown-on-chromogenic-media-by-maldi-tof-ms
#1
Petra Lüthje, Arthur B Pranada, Duncan Carruthers-Lay, Marc Desjardins, Olivier Gaillot, David Wareham, Holly Ciesielczuk, Volkan Özenci
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS...
March 4, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28259604/bacterial-contamination-of-ct-equipment-use-of-atp-detection-and-culture-results-to-target-quality-improvement
#2
John Childress, Debborah Burch, Cheryl Kucharski, Carol Young, Ella A Kazerooni, Matthew S Davenport
RATIONALE AND OBJECTIVE: This study aimed to evaluate the use of an adenosine triphosphate (ATP) monitoring system to minimize surface contamination on inpatient computed tomography (CT) scanners. METHODS: The bore, table, and wrap of two quaternary care inpatient CT scanners (load/scanner: ~ 30-40 CT examinations/day) were assayed with bacterial cultures and an ATP detection system during six prospective iterative plan-do-check-act improvement cycles from January 6, 2016 to October 12, 2016...
March 1, 2017: Academic Radiology
https://www.readbyqxmd.com/read/28254249/comparative-evaluation-of-a-new-commercial-media-the-chromagar%C3%A2-msupercarba%C3%A2-for-the-detection-of-carbapenemase-producing-enterobacteriaceae
#3
Ma'ayan Amar, Ohad Shalom, Amos Adler
A new chromogenic-based medium (mSuperCARBA™) was tested for screening carbapenemase-producing Enterobacteriaceae (CPE). mSuperCARBA™ was more sensitive (83%) in detecting CPE isolates (n=69, including KPC, NDM, OXA-48, VIM, and IMI) compared with CHROMAgar™-KPC (65%) and MacConkey agar with Imipenem (69%) with comparable specificity for non carbapenemase-producing, carbapenem-resistant Enterobacteriaceae (n=29).
February 11, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28243912/formation-of-biofilms-by-foodborne-pathogens-and-development-of-laboratory-in-vitro-model-for-the-study-of-campylobacter-genus-bacteria-based-on-these-biofilms
#4
N R Efimochkina, I B Bykova, Yu M Markova, Yu V Korotkevich, V V Stetsenko, L P Minaeva, S A Sheveleva
We analyzed the formation of biofilms by 7 strains of Campylobacter genus bacteria and 18 strains of Enterobacteriaceae genus bacteria that were isolated from plant and animal raw materials, from finished products, and swabs from the equipment of the food industry. Biofilm formation on glass plates, slides and coverslips, microtubes made of polymeric materials and Petri dishes, and polystyrene plates of different profiles were analyzed. When studying the process of films formation, different effects on bacterial populations were simulated, including variation of growth factor composition of culture media, technique of creating of anaerobiosis, and biocide treatment (active chlorine solutions in a concentration of 100 mg/dm(3))...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28242273/efficacy-and-acceptability-of-rectal-and-perineal-sampling-for-identifying-gastrointestinal-colonisation-with-esbl-enterobacteriaceae
#5
Eleonora Dyakova, Karen N Bisnauthsing, Antonio Querol-Rubiera, Amita Patel, Chioma Ahanonu, Olga Tosas Auguet, Jonathan D Edgeworth, Simon D Goldenberg, Jonathan A Otter
OBJECTIVES: We evaluated 'pre-laboratory' factors associated with the detection of ESBL-producing Enterobacteriaceae (ESBL-E) colonisation including anatomical site, and staff and patient factors. METHODS: All admissions to a large London hospital over three months were approached to provide rectal and perineal swabs, which were cultured for ESBL-E using chromogenic media. ESBL-E detection rates for patient or staff-collected rectal or perineal swabs were compared using McNemar Tests...
February 24, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28131671/comparison-of-two-rapid-biochemical-tests-and-four-chromogenic-selective-media-for-detection-of-carbapenemase-producing-gram-negative-bacteria
#6
Vladimira Hinić, Ivo Amrein, Sabrina Stammler, Judith Heckendorn, Dominik Meinel, Reno Frei, Adrian Egli
We evaluated RAPIDEC® CARBA NP, Neo-Rapid CARB, chromID® CARBA SMART (CARB/OXA), Brilliance™ CRE/ESBL, ChromArt CRE and BBL™ CHROMagar™ CPE for the detection of carbapenemase-producing bacteria. The analytical sensitivity of RAPIDEC® CARBA NP was better than that of Neo-Rapid CARB. A combination of carbapenemase and ESBL screening plates could be advantageous.
January 25, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28122803/a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#7
REVIEW
John D Perry
In the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. In the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including Pseudomonas aeruginosa, group B streptococci, Clostridium difficile, Campylobacter spp., and Yersinia enterocolitica. New media have also been developed to screen for pathogens with acquired antimicrobial resistance, including vancomycin-resistant enterococci, carbapenem-resistant Acinetobacter spp...
April 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28108122/-fast-methods-of-fungal-and-bacterial-identification-maldi-tof-mass-spectrometry-chromogenic-media
#8
María Siller-Ruiz, Sara Hernández-Egido, Fernando Sánchez-Juanes, José Manuel González-Buitrago, Juan Luis Muñoz-Bellido
MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency...
January 17, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28073895/evaluation-of-genotypic-and-phenotypic-methods-to-detect-carbapenemase-production-in-gram-negative-bacilli
#9
Allison R McMullen, Melanie L Yarbrough, Meghan A Wallace, Angela Shupe, Carey-Ann D Burnham
BACKGROUND: Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS: We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation method (CIM) and 2 chromogenic agars] and a molecular method (Cepheid GeneXpert Carba-R) to identify the mechanism of carbapenem resistance and the associated resistance genes (blaKPC, blaNDM, blaIMP, blaOXA-48-like, and blaVIM)...
March 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28064137/a-highly-selective-chromogenic-sensor-for-mn-2-turn-off-fluorometric-for-hg-2-ion-and-turn-on-fluorogenic-sensor-for-f-ion-with-the-practical-application
#10
Neha Gupta, Divya Singhal, Ashok Kumar Singh, Neetu Singh, U P Singh
A colorimetric and fluorometric probe (E)-2-((8-hydroxy-1,2,3,5,6,7-hexahydropyrido[3,2,1-ij]quinolin-9-yl)methylene)hydrazinecarbothioamide based on thiosemicarbazide and julolidine moieties has been synthesised in pure crystalline form and characterized by (1)H NMR, UV-vis, elemental analysis and single crystal XRD. The probe functioned as multitarget ion sensor, detect biologically important metal ions Hg(2+) and Mn(2+) in dual channel mode. Meanwhile, in mixed solvent media DMF/H2O [8:2], probe displayed selectivity for Hg(2+) over other cations by the emission spectrum...
January 4, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28007529/application-of-a-paper-based-device-containing-a-new-culture-medium-to-detect-vibrio-cholerae-in-water-samples-collected-in-haiti
#11
REVIEW
Romain Briquaire, Rita R Colwell, Jacques Boncy, Emmanuel Rossignol, Aline Dardy, Isabelle Pandini, François Villeval, Jean-Louis Machuron, Anwar Huq, Shah Rashed, Thierry Vandevelde, Christine Rozand
Cholera is now considered to be endemic in Haiti, often with increased incidence during rainy seasons. The challenge of cholera surveillance is exacerbated by the cost of sample collection and laboratory analysis. A diagnostic tool is needed that is low cost, easy-to-use, and able to detect and quantify Vibrio cholerae accurately in water samples within 18-24h, and perform reliably in remote settings lacking laboratory infrastructure and skilled staff. The two main objectives of this study were to develop and evaluate a new culture medium embedded in a new diagnostic tool (PAD for paper based analytical device) for detecting Vibrio cholerae from water samples collected in Haiti...
December 19, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27979140/development-of-a-laboratory-technique-for-the-evaluation-of-protease-enzymes-activity-in-goat-and-sheep-milk
#12
Raffaele Palomba, Giorgio Formisano, Assunta Arrichiello, Giuseppe Auriemma, Fiorella Sarubbi
The shelf life of milk and its products is strongly influenced by the quality of raw milk. Several bacteria present in raw milk can produce proteases: among them, those from psychotropic microorganisms (e.g. Bacillus subtilis), which produce enzymes under refrigeration, cause the most serious problem. The interaction of these enzymes with the milk proteins and the main proteolytic enzymes becomes important in determining the quality of the milk. An artificial substrate (azocasein) was used to quantify the enzyme activity through the release of a chromogenic product that was measured spectrophotometrically after clarifying the sample by the addition of trichloroacetic acid...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#13
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27934103/simultaneous-identification-and-antimicrobial-susceptibility-testing-of-multiple-uropathogens-on-a-microfluidic-chip-with-paper-supported-cell-culture-arrays
#14
Banglao Xu, Yan Du, Jinqiong Lin, Mingyue Qi, Bowen Shu, Xiaoxia Wen, Guangtie Liang, Bin Chen, Dayu Liu
A microfluidic chip was developed for one-step identification and antimicrobial susceptibility testing (AST) of multiple uropathogens. The polydimethylsiloxane (PDMS) microchip used had features of cell culture chamber arrays connected through a sample introduction channel. At the bottom of each chamber, a paper substrate preloaded with chromogenic media and antimicrobial agents was embedded. By integrating a hydrophobic membrane valve on the microchip, the urine sample can be equally distributed into and confined in individual chambers...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27873028/mcr-modern-colistin-resistance
#15
REVIEW
I Caniaux, A van Belkum, G Zambardi, L Poirel, M F Gros
Recently, plasmid-mediated and, therefore, transferable bacterial polymyxin resistance was discovered in strains from both humans and animals. Such a trait may widely spread geographically, while simultaneously crossing microbial species barriers. This may ultimately render the "last resort" polymyxin antibiotics therapeutically useless. Colistin is currently used to treat infections caused by Gram-negative carbapenemase producers and colistin resistance may lead to practical pan-antibiotic resistance. We here analyzed the medical and diagnostic consequences of (emerging) colistin resistance and propose pathways toward adequate diagnostics for timely detection of both asymptomatic carriage and infection...
March 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27871613/revisiting-catechol-derivatives-as-robust-chromogenic-hydrogen-donors-working-in-alkaline-media-for-peroxidase-mimetics
#16
Marcin Drozd, Mariusz Pietrzak, Jakub Pytlos, Elżbieta Malinowska
Colloidal noble metal-based nanoparticles are able to catalyze oxidation of chromogenic substrates by H2O2, similarly to peroxidases, even in basic media. However, lack of robust chromogens, which work in high pH impedes their real applications. Herein we demonstrate the applicability of selected catechol derivatives: bromopyrogallol red (BPR) and pyrogallol (PG) as chromogenic substrates for peroxidase-like activity assays, which are capable of working over wide range of pH, covering also basic values. Hyperbranched polyglycidol-stabilized gold nanoparticles (HBPG@AuNPs) were used as model enzyme mimetics...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27812488/identification-of-klebsiella-pneumoniae-carbapenemase-producing-klebsiella-oxytoca-in-clinical-isolates-in-tehran-hospitals-iran-by-chromogenic-medium-and-molecular-methods
#17
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
OBJECTIVES: Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods. METHODS: A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method...
October 2016: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/27784868/evaluation-of-chromogenic-medium-for-selective-isolation-of-yersinia
#18
Nguyen Khanh Thuan, Kamrun Naher, Ryoichi Kubo, Takahide Taniguchi, Hideki Hayashidani
Cefsulodin-irgasan-novobiocin agar (CIN) has been used as a selective agar to detect Yersinia in food or human patients; however, its components can inhibit the growth of some strains of Yersinia enterocolitica serovar O3 and Y. pseudotuberculosis. Recently, a new Yersinia selective agar, CHROMagar Yersinia enterocolitica (CAYe), was developed and evaluated as a novel selective agar for pathogenic Y. enterocolitica. In this research, a total of 251Yersinia strains (176 pathogenic Y. enterocolitica, 59 Y. pseudotuberculosis, and 16 non-pathogenic Yersinia) were cultured on both CIN and CAYe for comparison...
2016: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/27722941/pitfalls-and-capabilities-of-various-hydrogen-donors-in-evaluation-of-peroxidase-like-activity-of-gold-nanoparticles
#19
Marcin Drozd, Mariusz Pietrzak, Paweł G Parzuchowski, Elżbieta Malinowska
Catalytic nanomaterials, widely used as substitutes of peroxidase, exhibit unique properties, which are unattainable for native enzymes. However, their activity is usually examined by means of substrates developed and methods standardized for horseradish peroxidase (HRP). The aim of the presented work was to determine the scope of usefulness of chromogenic substrates for gold nanoparticle (AuNP) activity studies under conditions which significantly extend beyond the activity range of a native HRP. The applicability of chromogens such as 3,3'5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) beyond the typical range of pH, and for the samples of high concentration of hydrogen peroxide was examined...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27711274/vapour-induced-solid-state-c-h-bond-activation-for-the-clean-synthesis-of-an-organopalladium-biothiol-sensor
#20
Andrea Monas, Krunoslav Užarević, Ivan Halasz, Marina Juribašić Kulcsár, Manda Ćurić
Room-temperature accelerated aging in the solid state has been applied for atom- and energy-efficient activation of either one or two C-H bonds of azobenzene and methyl orange by palladium(ii) acetate. Organopalladium complexes are prepared in quantitative reactions without potentially harmful side products. Dicyclopalladated methyl orange is water-soluble and is a selective chromogenic biothiol sensor at physiologically-relevant micromolar concentrations in buffered aqueous media.
September 9, 2016: Chemical Communications: Chem Comm
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