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https://www.readbyqxmd.com/read/28073895/evaluation-of-genotypic-and-phenotypic-methods-to-detect-carbapenemase-production-in-gram-negative-bacilli
#1
Allison R McMullen, Melanie L Yarbrough, Meghan A Wallace, Angela Shupe, Carey-Ann D Burnham
BACKGROUND: Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS: We examined 189 carbapenem-resistant GNB (CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation method (CIM) and 2 chromogenic agars] and a molecular method (Cepheid GeneXpert Carba-R) to identify the mechanism of carbapenem resistance and the associated resistance genes (blaKPC, blaNDM, blaIMP, blaOXA-48-like, and blaVIM)...
January 10, 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28064137/a-highly-selective-chromogenic-sensor-for-mn-2-turn-off-fluorometric-for-hg-2-ion-and-turn-on-fluorogenic-sensor-for-f-ion-with-the-practical-application
#2
Neha Gupta, Divya Singhal, Ashok Kumar Singh, Neetu Singh, U P Singh
A colorimetric and fluorometric probe (E)-2-((8-hydroxy-1,2,3,5,6,7-hexahydropyrido[3,2,1-ij]quinolin-9-yl)methylene)hydrazinecarbothioamide based on thiosemicarbazide and julolidine moieties has been synthesised in pure crystalline form and characterized by (1)H NMR, UV-vis, elemental analysis and single crystal XRD. The probe functioned as multitarget ion sensor, detect biologically important metal ions Hg(2+) and Mn(2+) in dual channel mode. Meanwhile, in mixed solvent media DMF/H2O [8:2], probe displayed selectivity for Hg(2+) over other cations by the emission spectrum...
January 4, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/28007529/application-of-a-paper-based-device-containing-a-new-culture-medium-to-detect-vibrio-cholerae-in-water-samples-collected-in-haiti
#3
REVIEW
Romain Briquaire, Rita R Colwell, Jacques Boncy, Emmanuel Rossignol, Aline Dardy, Isabelle Pandini, François Villeval, Jean-Louis Machuron, Anwar Huq, Shah Rashed, Thierry Vandevelde, Christine Rozand
Cholera is now considered to be endemic in Haiti, often with increased incidence during rainy seasons. The challenge of cholera surveillance is exacerbated by the cost of sample collection and laboratory analysis. A diagnostic tool is needed that is low cost, easy-to-use, and able to detect and quantify Vibrio cholerae accurately in water samples within 18-24h, and perform reliably in remote settings lacking laboratory infrastructure and skilled staff. The two main objectives of this study were to develop and evaluate a new culture medium embedded in a new diagnostic tool (PAD for paper based analytical device) for detecting Vibrio cholerae from water samples collected in Haiti...
December 19, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27979140/development-of-a-laboratory-technique-for-the-evaluation-of-protease-enzymes-activity-in-goat-and-sheep-milk
#4
Raffaele Palomba, Giorgio Formisano, Assunta Arrichiello, Giuseppe Auriemma, Fiorella Sarubbi
The shelf life of milk and its products is strongly influenced by the quality of raw milk. Several bacteria present in raw milk can produce proteases: among them, those from psychotropic microorganisms (e.g. Bacillus subtilis), which produce enzymes under refrigeration, cause the most serious problem. The interaction of these enzymes with the milk proteins and the main proteolytic enzymes becomes important in determining the quality of the milk. An artificial substrate (azocasein) was used to quantify the enzyme activity through the release of a chromogenic product that was measured spectrophotometrically after clarifying the sample by the addition of trichloroacetic acid...
April 15, 2017: Food Chemistry
https://www.readbyqxmd.com/read/27942042/faecal-carriage-of-gram-negative-multidrug-resistant-bacteria-among-patients-hospitalized-in-two-centres-in-ulaanbaatar-mongolia
#5
Bayaraa Baljin, Ganbaatar Baldan, Battogtokh Chimeddorj, Khosbayar Tulgaa, Batbaatar Gunchin, Tsogtsaikhan Sandag, Klaus Pfeffer, Colin R MacKenzie, Andreas F Wendel
Gram-negative multidrug-resistant organisms (GN-MDRO) producing β-lactamases (ESBL, plasmid-mediated AmpC β-lactamases and carbapenemases) are increasingly reported throughout Asia. The aim of this surveillance study was to determine the rate of bacterial colonization in patients from two hospitals in the Mongolian capital Ulaanbaatar. Rectal swabs were obtained from patients referred to the National Traumatology and Orthopaedics Research Centre (NTORC) or the Burn Treatment Centre (BTC) between July and September 2014, on admission and again after 14 days...
2016: PloS One
https://www.readbyqxmd.com/read/27934103/simultaneous-identification-and-antimicrobial-susceptibility-testing-of-multiple-uropathogens-on-a-microfluidic-chip-with-paper-supported-cell-culture-arrays
#6
Banglao Xu, Yan Du, Jinqiong Lin, Mingyue Qi, Bowen Shu, Xiaoxia Wen, Guangtie Liang, Bin Chen, Dayu Liu
A microfluidic chip was developed for one-step identification and antimicrobial susceptibility testing (AST) of multiple uropathogens. The polydimethylsiloxane (PDMS) microchip used had features of cell culture chamber arrays connected through a sample introduction channel. At the bottom of each chamber, a paper substrate preloaded with chromogenic media and antimicrobial agents was embedded. By integrating a hydrophobic membrane valve on the microchip, the urine sample can be equally distributed into and confined in individual chambers...
December 6, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/27873028/mcr-modern-colistin-resistance
#7
REVIEW
I Caniaux, A van Belkum, G Zambardi, L Poirel, M F Gros
Recently, plasmid-mediated and, therefore, transferable bacterial polymyxin resistance was discovered in strains from both humans and animals. Such a trait may widely spread geographically, while simultaneously crossing microbial species barriers. This may ultimately render the "last resort" polymyxin antibiotics therapeutically useless. Colistin is currently used to treat infections caused by Gram-negative carbapenemase producers and colistin resistance may lead to practical pan-antibiotic resistance. We here analyzed the medical and diagnostic consequences of (emerging) colistin resistance and propose pathways toward adequate diagnostics for timely detection of both asymptomatic carriage and infection...
November 21, 2016: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/27871613/revisiting-catechol-derivatives-as-robust-chromogenic-hydrogen-donors-working-in-alkaline-media-for-peroxidase-mimetics
#8
Marcin Drozd, Mariusz Pietrzak, Jakub Pytlos, Elżbieta Malinowska
Colloidal noble metal-based nanoparticles are able to catalyze oxidation of chromogenic substrates by H2O2, similarly to peroxidases, even in basic media. However, lack of robust chromogens, which work in high pH impedes their real applications. Herein we demonstrate the applicability of selected catechol derivatives: bromopyrogallol red (BPR) and pyrogallol (PG) as chromogenic substrates for peroxidase-like activity assays, which are capable of working over wide range of pH, covering also basic values. Hyperbranched polyglycidol-stabilized gold nanoparticles (HBPG@AuNPs) were used as model enzyme mimetics...
December 15, 2016: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27812488/identification-of-klebsiella-pneumoniae-carbapenemase-producing-klebsiella-oxytoca-in-clinical-isolates-in-tehran-hospitals-iran-by-chromogenic-medium-and-molecular-methods
#9
Majid Validi, Mohammad Mehdi Soltan Dallal, Masoumeh Douraghi, Jalil Fallah Mehrabadi, Abbas Rahimi Foroushani
OBJECTIVES: Production of carbapenemase, especially Klebsiella pneumoniae carbapenemases (KPC), is one of the antibiotic resistance mechanisms of Enterobacteriaceae such as Klebsiella oxytoca. This study aimed to investigate and identify KPC-producing K. oxytoca isolates using molecular and phenotypic methods. METHODS: A total of 75 isolates of K. oxytoca were isolated from various clinical samples, and were verified as K. oxytoca after performing standard microbiological tests and using a polymerase chain reaction (PCR) method...
October 2016: Osong Public Health and Research Perspectives
https://www.readbyqxmd.com/read/27784868/evaluation-of-chromogenic-medium-for-selective-isolation-of-yersinia
#10
Nguyen Khanh Thuan, Kamrun Naher, Ryoichi Kubo, Takahide Taniguchi, Hideki Hayashidani
Cefsulodin-irgasan-novobiocin agar (CIN) has been used as a selective agar to detect Yersinia in food or human patients; however, its components can inhibit the growth of some strains of Yersinia enterocolitica serovar O3 and Y. pseudotuberculosis. Recently, a new Yersinia selective agar, CHROMagar Yersinia enterocolitica (CAYe), was developed and evaluated as a novel selective agar for pathogenic Y. enterocolitica. In this research, a total of 251Yersinia strains (176 pathogenic Y. enterocolitica, 59 Y. pseudotuberculosis, and 16 non-pathogenic Yersinia) were cultured on both CIN and CAYe for comparison...
2016: Shokuhin Eiseigaku Zasshi. Journal of the Food Hygienic Society of Japan
https://www.readbyqxmd.com/read/27722941/pitfalls-and-capabilities-of-various-hydrogen-donors-in-evaluation-of-peroxidase-like-activity-of-gold-nanoparticles
#11
Marcin Drozd, Mariusz Pietrzak, Paweł G Parzuchowski, Elżbieta Malinowska
Catalytic nanomaterials, widely used as substitutes of peroxidase, exhibit unique properties, which are unattainable for native enzymes. However, their activity is usually examined by means of substrates developed and methods standardized for horseradish peroxidase (HRP). The aim of the presented work was to determine the scope of usefulness of chromogenic substrates for gold nanoparticle (AuNP) activity studies under conditions which significantly extend beyond the activity range of a native HRP. The applicability of chromogens such as 3,3'5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) beyond the typical range of pH, and for the samples of high concentration of hydrogen peroxide was examined...
November 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27711274/vapour-induced-solid-state-c-h-bond-activation-for-the-clean-synthesis-of-an-organopalladium-biothiol-sensor
#12
Andrea Monas, Krunoslav Užarević, Ivan Halasz, Marina Juribašić Kulcsár, Manda Ćurić
Room-temperature accelerated aging in the solid state has been applied for atom- and energy-efficient activation of either one or two C-H bonds of azobenzene and methyl orange by palladium(ii) acetate. Organopalladium complexes are prepared in quantitative reactions without potentially harmful side products. Dicyclopalladated methyl orange is water-soluble and is a selective chromogenic biothiol sensor at physiologically-relevant micromolar concentrations in buffered aqueous media.
September 9, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27698166/validation-of-three-automated-assays-for-total-antioxidant-capacity-determination-in-canine-serum-samples
#13
Camila Peres Rubio, Josefa Hernández-Ruiz, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Marino Bañón Arnao, José Joaquín Ceron
We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•(+)) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced...
November 2016: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/27582518/culture-of-urine-specimens-by-use-of-chromid-cps-elite-medium-can-expedite-escherichia-coli-identification-and-reduce-hands-on-time-in-the-clinical-laboratory
#14
Melanie L Yarbrough, Meghan A Wallace, Cynthia Marshall, Erin Mathias, C A Burnham
Urine is one of the most common specimen types submitted to the clinical microbiology laboratory; the use of chromogenic agar is one method by which the laboratory might expedite culture results and reduce hands-on time and materials required for urine culture analysis. The objective of our study was to compare chromID CPS Elite (bioMérieux), a chromogenic medium, to conventional primary culture medium for evaluation of urine specimens. Remnant urine specimens (n = 200) were inoculated into conventional media and into chromID CPS Elite agar (chromID)...
November 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27562853/chromogenic-media-for-mrsa-diagnostics
#15
REVIEW
Zhenbo Xu, Yuchao Hou, Brian M Peters, Dingqiang Chen, Bing Li, Lin Li, Mark E Shirtliff
For the past decade, a number of chromogenic media for methicillin-resistance Staphylococcus aureus (MRSA) detection have been developed and applied, including Oxoid Brilliance™ MRSA, CHROMagar™ MRSA, BBL™ CHROMagar™ MRSA, MRSASelect and chromID MRSA. The advantages of these chromogenic media offers direct detection of visible staphylococcal colonies, coupled with the use of chromogenic enzymatic substrates that can be hydrolyzed by S. aureus to confirm species or strain identification. BBL™ CHROMagar™ MRSA and MRSASelect are designed for detection of nasal colonization by MRSA, while CHROMagar™ MRSA, Oxoid Brilliance™ MRSA and chromID MRSA are readily applied in bacterial screening...
November 2016: Molecular Biology Reports
https://www.readbyqxmd.com/read/27534258/performance-of-the-chromid-salmonella-elite-chromogenic-agar-in-comparison-with-chromagar%C3%A2-salmonella-oxoid%C3%A2-brilliance%C3%A2-salmonella-and-hektoen-agars-for-the-isolation-of-salmonella-from-stool-specimens
#16
Delphine Martiny, Anne Dediste, Claire Anglade, Linda Vlaes, Catherine Moens, Souad Mohamed, Olivier Vandenberg
chromID™ Salmonella Elite is compared with 3 culture media commonly used for Salmonella isolation from stool specimens. As results were equivalent to other chromogenic media (100% sensitivity, 98% specificity), only financial arguments should guide the choice for a medium with respect to another.
October 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27525391/-investigation-of-the-serotype-distribution-biofilm-production-and-antibiotic-susceptibilities-of-group-b-streptococci-isolated-from-urinary-samples
#17
Sevinç Baba, Mustafa Derya Aydın
Streptococcus agalactiae (Group B streptococcus, GBS), a member of normal flora of human gastrointestinal and genitourinary systems, is a leading cause of sepsis, meningitis, and pneumonia particularly in newborn. GBS can also cause severe infections in pregnant women and adults with underlying disease, as well as mild diseases, such as urinary tract infections (UTIs). GBS strains exhibit 10 different serotypes, and the identification of serotype distribution is important epidemiologically. The role of biofilm production is one of the virulence factors that has been discussed in the pathogenesis of GBS infections...
July 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27516471/universal-hospital-admission-screening-for-carbapenemase-producing-organisms-in-a-low-prevalence-setting
#18
Jonathan A Otter, Eleonora Dyakova, Karen N Bisnauthsing, Antonio Querol-Rubiera, Amita Patel, Chioma Ahanonu, Olga Tosas Auguet, Jonathan D Edgeworth, Simon D Goldenberg
BACKGROUND: Carbapenemase-producing Enterobacteriaceae (CPE) are an emerging threat for healthcare providers worldwide. OBJECTIVES: To determine CPE carriage rates and risk factors in an unselected hospital cohort at the time of admission. METHODS: We approached 4567 patients within 72 h of admission to provide a rectal swab and answer a questionnaire on risk factors for carriage. Rectal swabs were cultured for carbapenem-resistant organisms on chromogenic and non-chromogenic agar, and tested for carbapenemase production by PCR (Check-Direct CPE)...
August 11, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27456779/epizootiological-characteristics-of-viable-bacteria-and-fungi-in-indoor-air-from-porcine-chicken-or-bovine-husbandry-confinement-buildings
#19
Katharine Roque, Gyeong-Dong Lim, Ji-Hoon Jo, Kyung-Min Shin, Eun-Seob Song, Ravi Gautam, Chang-Yul Kim, Kyungsuk Lee, Seungwon Shin, Han-Sang Yoo, Yong Heo, Hyoung-Ah Kim
Microorganisms found in bioaerosols from animal confinement buildings not only foster the risk of spreading diseases among livestock buildings, but also pose health hazards to farm workers and nearby residents. This study identified the various microorganisms present in the air of swine, chicken, and cattle farms with different kinds of ventilation conditions in Korea. Microbial air samples were collected onto Petri dishes with bacterial or fungal growth media using a cascade impactor. Endotoxin levels in total dust were determined by the limulus amebocyte lysate kinetic QCL method...
December 30, 2016: Journal of Veterinary Science
https://www.readbyqxmd.com/read/27413193/automatic-digital-analysis-of-chromogenic-media-for-vancomycin-resistant-enterococcus-screens-using-copan-wasplab
#20
Matthew L Faron, Blake W Buchan, Christopher Coon, Theo Liebregts, Anita van Bree, Arjan R Jansz, Genevieve Soucy, John Korver, Nathan A Ledeboer
Vancomycin-resistant enterococci (VRE) are an important cause of health care-acquired infections (HAIs). Studies have shown that active surveillance of high-risk patients for VRE colonization can aid in reducing HAIs; however, these screens generate a significant cost to the laboratory and health care system. Digital imaging capable of differentiating negative and "nonnegative" chromogenic agar can reduce the labor cost of these screens and potentially improve patient care. In this study, we evaluated the performance of the WASPLab Chromogenic Detection Module (CDM) (Copan, Brescia, Italy) software to analyze VRE chromogenic agar and compared the results to technologist plate reading...
October 2016: Journal of Clinical Microbiology
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