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Chromogenic media

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https://www.readbyqxmd.com/read/28604906/a-selective-emissive-chromogenic-and-fluorogenic-seleno-coumarin-probe-for-cu-2-detection-in-aprotic-media
#1
A C Gonçalves, J Luis Capelo, C Lodeiro, A A Dos Santos
A new selenium containing coumarin (compound 7) was designed and synthesized from the amide linkage between coumarin-519 (6) and 2-(butylselanyl)ethanamine (5). The molecular structure of 7 was accurately characterized, and its photophysical properties in acetonitrile, ethanol and chloroform solutions were studied by absorption, stationary and time-resolved fluorescence spectroscopies. Changes in the solvent polarity affected the Stokes shift, quantum yields and lifetime of the excited states. The spectroscopic behavior of compound 7 was evaluated in the presence of different monovalent, divalent and trivalent metallic cations (Na(+), K(+), Ca(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), Pb(2+), Hg(2+), Hg(+), Ag(+), Al(3+), Fe(3+), Ga(3+) and Cr(3+)) in acetonitrile solution...
June 12, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28594779/clinical-validation-of-a-test-for-the-diagnosis-of-vaginitis
#2
Charlotte A Gaydos, Sajo Beqaj, Jane R Schwebke, Joel Lebed, Bonnie Smith, Thomas E Davis, Kenneth H Fife, Paul Nyirjesy, Timothy Spurrell, Dorothy Furgerson, Jenell Coleman, Sonia Paradis, Charles K Cooper
OBJECTIVE: Vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. A new molecular test assays the vaginal microbiome and organisms that cause three common infections. The objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. The primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of vaginitis and compare clinician-collected with self-collected swabs...
June 6, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28559163/a-cost-saving-strategy-for-processing-isolated-uropathogens-in-community-acquired-urinary-tract-infections
#3
Juan Manuel Sánchez-Calvo, José Luís de Francisco, Eva Torres-Martos, Juan Carlos Alados Arboledas, Maria Dolores López Prieto
Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total)...
May 27, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28549606/variable-performance-of-four-commercial-chromogenic-media-for-detection-of-methicillin-resistant-staphylococcus-aureus-isolates-harbouring-mecc
#4
Céline Dupieux, Camille Kolenda, Anders Rhod Larsen, Bruno Pichon, Mark Holmes, Michèle Bes, Christopher Teale, Elizabeth Dickson, Robert Hill, Robert Skov, Angela Kearns, Frédéric Laurent
In this study, the performances of four methicillin-resistant Staphylococcus aureus (MRSA) chromogenic screening media were compared for detecting mecC-positive MRSA. Using 111 clinical isolates representative of the various mecC-MRSA clones in Europe, chromID(®) MRSA (bioMérieux) and Brilliance(TM) MRSA 2 (Oxoid Ltd.) showed higher sensitivity (99.1% and 97.3%, respectively) than BBL(TM) CHROMagar(®) MRSA II (BD Diagnostics) and MRSASelect(TM) (Bio-Rad) (79.3% and 63.1%, respectively) (P < 0.0001). These findings have important implications for effective public health diagnostics and epidemiological surveillance of MRSA...
May 23, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28511380/fungal-profile-of-vulvovaginal-candidiasis-in-a-tertiary-care-hospital
#5
Krishnapriya Kalaiarasan, Rakesh Singh, Latha Chaturvedula
INTRODUCTION: Vulvovaginal Candidiasis (VVC) is a common medical health problem of adult women. It is most commonly caused by Candida albicans. But there is a change in fungal profile. Sabouraud's Dextrose Agar (SDA) is the most common culture medium used where mixed fungal infection may be missed. It can be detected easily by using chromogenic culture medium. AIM: To know the fungal profile of vulvovaginal candidiasis using Candida CHROMagar and antifungal susceptibility pattern in patients attending tertiary care hospital...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28505508/phosphatase-activities-of-a-microepiphytic-community-during-a-bloom-of-ostreopsis-cf-ovata-in-the-northern-adriatic-sea
#6
Stefano Accoroni, Cecilia Totti, Emanuela Razza, Roberta Congestri, Alessandra Campanelli, Mauro Marini, Neil Thomas William Ellwood
It is becoming more apparent that increased organic nutrient loads deriving from anthropogenic activities and natural processes frequently cause the eutrophication of coastal waters. Concurrently, an increasing number of phototrophs have been shown to make use of organic nutrients, mainly through indirect studies of surface enzyme activities or through direct studies of growth in media containing organic-only nutrients. The potential utilization of dissolved organic phosphorus (DOP) by microepiphytic-mats associated with frequently problematic, toxic dinoflagellate Ostreopsis cf...
May 8, 2017: Water Research
https://www.readbyqxmd.com/read/28467187/evaluation-of-two-standard-and-two-chromogenic-selective-media-for-optimal-growth-and-enumeration-of-isolates-of-16-unique-bacillus-species
#7
M Shahjahan Kabir, Ying-Hsin Hsieh, Steven Simpson, Khalil Kerdahi, Irshad M Sulaiman
The genus Bacillus is a group of gram-positive endospore-forming bacteria that can cause food poisoning and diarrheal illness in humans. A wide range of food products have been linked to foodborne outbreaks associated with these opportunistic pathogens. The U.S. Food and Drug Administration recommends (in their Bacteriological Analytical Manual) the use of Bacara or mannitol egg yolk polymyxin (MYP) agar plates and the most-probable-number (MPN) method for enumeration and confirmation of Bacillus cereus and related species isolated from foods, sporadic cases, outbreaks, and routine environmental surveillance samples...
June 2017: Journal of Food Protection
https://www.readbyqxmd.com/read/28444620/environmental-pollution-with-antimicrobial-agents-from-bulk-drug-manufacturing-industries-in-hyderabad-south-india-is-associated-with-dissemination-of-extended-spectrum-beta-lactamase-and-carbapenemase-producing-pathogens
#8
Christoph Lübbert, Christian Baars, Anil Dayakar, Norman Lippmann, Arne C Rodloff, Martina Kinzig, Fritz Sörgel
PURPOSE: High antibiotic and antifungal concentrations in wastewater from anti-infective drug production may exert selection pressure for multidrug-resistant (MDR) pathogens. We investigated the environmental presence of active pharmaceutical ingredients and their association with MDR Gram-negative bacteria in Hyderabad, South India, a major production area for the global bulk drug market. METHODS: From Nov 19 to 28, 2016, water samples were collected from the direct environment of bulk drug manufacturing facilities, the vicinity of two sewage treatment plants, the Musi River, and habitats in Hyderabad and nearby villages...
April 26, 2017: Infection
https://www.readbyqxmd.com/read/28419503/comparison-of-the-quantitative-dry-culture-methods-with-both-conventional-media-and-most-probable-number-method-for-the-enumeration-of-coliforms-and-escherichia-coli-coliforms-in-food
#9
H Teramura, K Sota, M Iwasaki, H Ogihara
Sanita-kun™ CC (coliform count) and EC (Escherichia coli/coliform count), sheet quantitative culture systems which can avoid chromogenic interference by lactase in food, were evaluated in comparison with conventional methods for these bacteria. Based on the results of inclusivity and exclusivity studies using 77 micro-organisms, sensitivity and specificity of both Sanita-kun™ met the criteria for ISO 16140. Both media were compared with deoxycholate agar, violet red bile agar, Merck Chromocult™ coliform agar (CCA), 3M Petrifilm™ CC and EC (PEC) and 3-tube MPN, as reference methods, in 100 naturally contaminated food samples...
April 17, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28418715/evaluation-of-selective-enrichment-and-chromogenic-media-for-salmonella-detection-in-raw-shell-egg-contents-with-a-low-microbial-load
#10
Soo-Kyoung Lee, Kwang-Young Song, Jung-Whan Chon, Dong-Hyeon Kim, Kun-Ho Seo
The current study was conducted to evaluate the ability to recover Salmonella from shell egg contents by culture methods. A total of 4,000 eggs were obtained from a grading and packing center located in the Gyeonggi Province of South Korea, and 200 samples were created by pooling 20 broken eggs. The pooled samples were held at room temperature for 4 d before a 25-mL aliquot of each pool was added to 225 mL of modified trypticase soy broth (mTSB) and incubated at 35°C for 24 ± 2 h. A loopful of the culture was streaked onto chromogenic Druggan-Forsythe-Iversen (DFI) agar and incubated at 36 ± 1°C for 18-24 h...
April 18, 2017: Foodborne Pathogens and Disease
https://www.readbyqxmd.com/read/28267570/identification-of-microorganisms-grown-on-chromogenic-media-by-maldi-tof-ms
#11
Petra Lüthje, Arthur B Pranada, Duncan Carruthers-Lay, Marc Desjardins, Olivier Gaillot, David Wareham, Holly Ciesielczuk, Volkan Özenci
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS...
March 4, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28259604/bacterial-contamination-of-ct-equipment-use-of-atp-detection-and-culture-results-to-target-quality-improvement
#12
John Childress, Debborah Burch, Cheryl Kucharski, Carol Young, Ella A Kazerooni, Matthew S Davenport
RATIONALE AND OBJECTIVE: This study aimed to evaluate the use of an adenosine triphosphate (ATP) monitoring system to minimize surface contamination on inpatient computed tomography (CT) scanners. METHODS: The bore, table, and wrap of two quaternary care inpatient CT scanners (load/scanner: ~ 30-40 CT examinations/day) were assayed with bacterial cultures and an ATP detection system during six prospective iterative plan-do-check-act improvement cycles from January 6, 2016 to October 12, 2016...
March 1, 2017: Academic Radiology
https://www.readbyqxmd.com/read/28254249/comparative-evaluation-of-a-new-commercial-media-the-chromagar%C3%A2-msupercarba%C3%A2-for-the-detection-of-carbapenemase-producing-enterobacteriaceae
#13
Ma'ayan Amar, Ohad Shalom, Amos Adler
A new chromogenic-based medium (mSuperCARBA™) was tested for screening carbapenemase-producing Enterobacteriaceae (CPE). mSuperCARBA™ was more sensitive (83%) in detecting CPE isolates (n=69, including KPC, NDM, OXA-48, VIM, and IMI) compared with CHROMAgar™-KPC (65%) and MacConkey agar with Imipenem (69%) with comparable specificity for non carbapenemase-producing, carbapenem-resistant Enterobacteriaceae (n=29).
May 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28243912/formation-of-biofilms-by-foodborne-pathogens-and-development-of-laboratory-in-vitro-model-for-the-study-of-campylobacter-genus-bacteria-based-on-these-biofilms
#14
N R Efimochkina, I B Bykova, Yu M Markova, Yu V Korotkevich, V V Stetsenko, L P Minaeva, S A Sheveleva
We analyzed the formation of biofilms by 7 strains of Campylobacter genus bacteria and 18 strains of Enterobacteriaceae genus bacteria that were isolated from plant and animal raw materials, from finished products, and swabs from the equipment of the food industry. Biofilm formation on glass plates, slides and coverslips, microtubes made of polymeric materials and Petri dishes, and polystyrene plates of different profiles were analyzed. When studying the process of films formation, different effects on bacterial populations were simulated, including variation of growth factor composition of culture media, technique of creating of anaerobiosis, and biocide treatment (active chlorine solutions in a concentration of 100 mg/dm(3))...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28242273/efficacy-and-acceptability-of-rectal-and-perineal-sampling-for-identifying-gastrointestinal-colonization-with-extended-spectrum-%C3%AE-lactamase-enterobacteriaceae
#15
E Dyakova, K N Bisnauthsing, A Querol-Rubiera, A Patel, C Ahanonu, O Tosas Auguet, J D Edgeworth, S D Goldenberg, J A Otter
OBJECTIVES: We evaluated 'pre-laboratory' factors associated with the detection of extended spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) colonization including anatomical site, and staff and patient factors. METHODS: All admissions to a large London hospital over 3 months were approached to provide rectal and perineal swabs, which were cultured for ESBL-E using chromogenic media. ESBL-E detection rates for patient- or staff-collected rectal or perineal swabs were compared using McNemar tests...
February 24, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28131671/comparison-of-two-rapid-biochemical-tests-and-four-chromogenic-selective-media-for-detection-of-carbapenemase-producing-gram-negative-bacteria
#16
Vladimira Hinić, Ivo Amrein, Sabrina Stammler, Judith Heckendorn, Dominik Meinel, Reno Frei, Adrian Egli
We evaluated RAPIDEC® CARBA NP, Neo-Rapid CARB, chromID® CARBA SMART (CARB/OXA), Brilliance™ CRE/ESBL, ChromArt CRE and BBL™ CHROMagar™ CPE for the detection of carbapenemase-producing bacteria. The analytical sensitivity of RAPIDEC® CARBA NP was better than that of Neo-Rapid CARB. A combination of carbapenemase and ESBL screening plates could be advantageous.
January 25, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28122803/a-decade-of-development-of-chromogenic-culture-media-for-clinical-microbiology-in-an-era-of-molecular-diagnostics
#17
REVIEW
John D Perry
In the last 25 years, chromogenic culture media have found widespread application in diagnostic clinical microbiology. In the last decade, the range of media available to clinical laboratories has expanded greatly, allowing specific detection of additional pathogens, including Pseudomonas aeruginosa, group B streptococci, Clostridium difficile, Campylobacter spp., and Yersinia enterocolitica. New media have also been developed to screen for pathogens with acquired antimicrobial resistance, including vancomycin-resistant enterococci, carbapenem-resistant Acinetobacter spp...
April 2017: Clinical Microbiology Reviews
https://www.readbyqxmd.com/read/28108122/fast-methods-of-fungal-and-bacterial-identification-maldi-tof-mass-spectrometry-chromogenic-media
#18
María Siller-Ruiz, Sara Hernández-Egido, Fernando Sánchez-Juanes, José Manuel González-Buitrago, Juan Luis Muñoz-Bellido
MALDI-TOF mass spectrometry is now a routine resource in Clinical Microbiology, because of its speed and reliability in the identification of microorganisms. Its performance in the identification of bacteria and yeasts is perfectly contrasted. The identification of mycobacteria and moulds is more complex, due to the heterogeneity of spectra within each species. The methodology is somewhat more complex, and expanding the size of species libraries, and the number of spectra of each species, will be crucial to achieve greater efficiency...
May 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28073895/evaluation-of-genotypic-and-phenotypic-methods-to-detect-carbapenemase-production-in-gram-negative-bacilli
#19
Allison R McMullen, Melanie L Yarbrough, Meghan A Wallace, Angela Shupe, Carey-Ann D Burnham
BACKGROUND: Carbapenemase-producing gram-negative bacteria (CP-GNB) are an urgent and expanding public health threat. Rapid and accurate identification of these organisms facilitates infection prevention efforts in healthcare facilities. The objective of our study was to evaluate methods to detect and identify CP-GNB. METHODS: We examined 189 carbapenem-resistant GNB(CR-GNB), including Enterobacteriaceae, Pseudomonas aeruginosa, and Acinetobacter baumannii complex, using 3 different methods: 2 methods to screen isolates of GNB for carbapenemase production [the carbapenem inactivation method (CIM) and 2 chromogenic agars] and a molecular method (Cepheid GeneXpert Carba-R) to identify the mechanism of carbapenem resistance and the associated resistance genes (blaKPC, blaNDM, blaIMP, blaOXA-48-like, and blaVIM)...
March 2017: Clinical Chemistry
https://www.readbyqxmd.com/read/28064137/a-highly-selective-chromogenic-sensor-for-mn-2-turn-off-fluorometric-for-hg-2-ion-and-turn-on-fluorogenic-sensor-for-f-ion-with-the-practical-application
#20
Neha Gupta, Divya Singhal, Ashok Kumar Singh, Neetu Singh, U P Singh
A colorimetric and fluorometric probe (E)-2-((8-hydroxy-1,2,3,5,6,7-hexahydropyrido[3,2,1-ij]quinolin-9-yl)methylene)hydrazinecarbothioamide based on thiosemicarbazide and julolidine moieties has been synthesised in pure crystalline form and characterized by (1)H NMR, UV-vis, elemental analysis and single crystal XRD. The probe functioned as multitarget ion sensor, detect biologically important metal ions Hg(2+) and Mn(2+) in dual channel mode. Meanwhile, in mixed solvent media DMF/H2O [8:2], probe displayed selectivity for Hg(2+) over other cations by the emission spectrum...
January 4, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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