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https://www.readbyqxmd.com/read/28633083/adsorption-of-cu-ii-pb-ii-and-cr-vi-from-aqueous-solutions-using-black-wattle-tannin-immobilized-nanocellulose
#1
Qinghua Xu, Yulu Wang, Liqiang Jin, Yu Wang, Menghua Qin
A novel nanocomposite based on black wattle (BW) tannin and nanocellulose was prepared and applied in heavy metal ions adsorptive removal from aqueous solutions. Firstly, nanocrystalline cellulose was oxidized by sodium periodate to get dialdehyde nanocellulose (DANC). BW tannin was then covalently immobilized onto DANC, which was used as both the matrix and crosslinker, to obtain tannin-nanocellulose (TNCC) composite. The resulting nanocomposite was characterized using FTIR, AFM, and TG. The successful immobilization was confirmed by the chromogenic reaction between FeCl3 and TNCC and FT-IR analysis...
June 17, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28627816/spectrophotometric-determination-of-selenium-through-triiodide-anion
#2
Otilia Bizerea-Spiridon, Radu Nartita, Alexandru F Rogobete, Adina Negrea, Ramona Stroescu, Teofana O Bizerea, Constantin Ilie, Otilia Marginean
BACKGROUND: Selenium is a chemical element found in the human body that plays a crucial role in its regulation. Depending on the concentration, it may have beneficial or have toxic effects. Selenium is incorporated as selenocysteine amino acid residue in selenoproteins which play an important role in many biological functions: anti-oxidant defense, regulation of the immune function and of the inflammatory response, metabolism of thyroid hormones, functioning of the central nervous system, biosynthesis of DNA and RNA, fertility, and reproduction...
May 1, 2017: Clinical Laboratory
https://www.readbyqxmd.com/read/28624271/coagulase-negative-staphylococci-species-affect-biofilm-formation-of-other-coagulase-negative-and-coagulase-positive-staphylococci
#3
Coralie Goetz, Yannick D N Tremblay, Daphnée Lamarche, Andréanne Blondeau, Annie M Gaudreau, Josée Labrie, François Malouin, Mario Jacques
Coagulase-negative staphylococci (CNS) are considered to be commensal bacteria in humans and animals, but are now also recognized as etiological agents in several infections, including bovine mastitis. Biofilm formation appears to be an important factor in CNS pathogenicity. Furthermore, some researchers have proposed that CNS colonization of the intramammary environment has a protective effect against other pathogens. The mechanisms behind the protective effect of CNS have yet to be characterized. The aim of this study was to evaluate the effect of CNS isolates with a weak-biofilm phenotype on the biofilm formation of other staphylococcal isolates...
June 14, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28621381/the-methodology-applied-in-dpph-abts-and-folin-ciocalteau-assays-has-a-large-influence-on-the-determined-antioxidant-potential
#4
Helena Abramovič, Blaž Grobin, Nataša Poklar, Blaž Cigić
Antioxidant potential (AOP) is not only the property of the matrix analyzed but also depends greatly on the methodology used. The chromogenic radicals 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and Folin-Ciocalteu (FC) assay were applied to estimate how the method and the composition of the assay solvent influence the AOP determined for coffee, tea, beer, apple juice and dietary supplements. Large differences between the AOP values depending on the reaction medium were observed, with the highest AOP determined mostly in the FC assay...
June 2017: Acta Chimica Slovenica
https://www.readbyqxmd.com/read/28619613/does-supplemental-photodynamic-therapy-optimize-the-disinfection-of-bacteria-and-endotoxins-in-one-visit-and-two-visit-root-canal-therapy-a-randomized-clinical-trial
#5
Diego G D Rabello, Bruna J M Corazza, Luciana L Ferreira, Mauro P Santamaria, Ana P M Gomes, Frederico C Martinho
AIM: to evaluate the effectiveness of supplemental photodynamic therapy (PDT) in optimizing the removal of bacteria and endotoxins from primarily infected root canals after one-visit and two-visit treatments. METHODOLOGY: Twenty-four primarily infected root canals with apical periodontitis were selected and randomly divided into one-visit (n=12) and two-visit treatment groups (n=12). Chemo-mechanical preparation (CMP) was performed by using the single-file reciprocating technique+2...
June 12, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28616595/a-rare-case-of-anal-carcinosarcoma-with-human-papilloma-virus-infection-in-both-biphasic-tumor-elements-an-immunohistochemical-molecular-and-ultrastructural-study
#6
Richard A Hickman, Azore-Dee Bradshaw, Nicholas Cassai, Antonio Galvao Neto, David Zhou, Tinghao Fu, Peng Lee, Zhiheng Pei, Rosemary Wieczorek
Carcinosarcoma of the anus is rare and has yet to be reportedly associated with the keratinocyte-specific Human Papilloma Virus (HPV). We describe a case of anal carcinosarcoma with HPV infection in both the epithelial and mesenchymal components of the tumor by immunohistochemistry, chromogenic in-situ hybridization (CISH) and further supported by electron microscopy (EM). Microscopic examination of the tumor showed nests of poorly-differentiated invasive squamous cell carcinoma with basaloid features intermixed with a hypercellular, atypical spindle cell proliferation...
December 2016: Papillomavirus Research
https://www.readbyqxmd.com/read/28611940/involvement-of-chromatin-remodeling-genes-and-the-rho-gtpases-rhob-and-cdc42-in-ovarian-clear-cell-carcinoma
#7
Nicolai Skovbjerg Arildsen, Jenny-Maria Jönsson, Katarina Bartuma, Anna Ebbesson, Sofia Westbom-Fremer, Anna Måsbäck, Susanne Malander, Mef Nilbert, Ingrid A Hedenfalk
OBJECTIVE: Ovarian clear cell carcinomas (OCCCs) constitute a rare ovarian cancer subtype with distinct clinical features, but may nonetheless be difficult to distinguish morphologically from other subtypes. There is limited knowledge of genetic events driving OCCC tumorigenesis beyond ARID1A, which is reportedly mutated in 30-50% of OCCCs. We aimed to further characterize OCCCs by combined global transcriptional profiling and targeted deep sequencing of a panel of well-established cancer genes...
2017: Frontiers in Oncology
https://www.readbyqxmd.com/read/28604906/a-selective-emissive-chromogenic-and-fluorogenic-seleno-coumarin-probe-for-cu-2-detection-in-aprotic-media
#8
A C Gonçalves, J Luis Capelo, C Lodeiro, A A Dos Santos
A new selenium containing coumarin (compound 7) was designed and synthesized from the amide linkage between coumarin-519 (6) and 2-(butylselanyl)ethanamine (5). The molecular structure of 7 was accurately characterized, and its photophysical properties in acetonitrile, ethanol and chloroform solutions were studied by absorption, stationary and time-resolved fluorescence spectroscopies. Changes in the solvent polarity affected the Stokes shift, quantum yields and lifetime of the excited states. The spectroscopic behavior of compound 7 was evaluated in the presence of different monovalent, divalent and trivalent metallic cations (Na(+), K(+), Ca(2+), Co(2+), Ni(2+), Cu(2+), Zn(2+), Cd(2+), Pb(2+), Hg(2+), Hg(+), Ag(+), Al(3+), Fe(3+), Ga(3+) and Cr(3+)) in acetonitrile solution...
June 12, 2017: Photochemical & Photobiological Sciences
https://www.readbyqxmd.com/read/28598339/comparison-of-mi-chromocult-%C3%A2-coliform-and-compass-cc-chromogenic-culture-based-methods-to-detect-escherichia-coli-and-total-coliforms-in-water-using-16s-rrna-sequencing-for-colony-identification
#9
Andrée F Maheux, Sébastien Bouchard, Ève Bérubé, Michel G Bergeron
The MI, Chromocult(®) coliform, and Compass CC chromogenic culture-based methods used to assess water quality by the detection of Escherichia coli and total coliforms were compared in terms of their specificity and sensitivity, using 16S rRNA sequencing for colony identification. A sewage water sample was divided in 2-μL subsamples for testing by all three culture-based methods. All growing colonies were harvested and subjected to 16S rRNA sequencing. Test results showed that all E. coli colonies were correctly identified by all three methods, for a specificity and a sensitivity of 100%...
June 2017: Journal of Water and Health
https://www.readbyqxmd.com/read/28594779/clinical-validation-of-a-test-for-the-diagnosis-of-vaginitis
#10
Charlotte A Gaydos, Sajo Beqaj, Jane R Schwebke, Joel Lebed, Bonnie Smith, Thomas E Davis, Kenneth H Fife, Paul Nyirjesy, Timothy Spurrell, Dorothy Furgerson, Jenell Coleman, Sonia Paradis, Charles K Cooper
OBJECTIVE: Vaginitis may be diagnosed as bacterial vaginosis, vulvovaginal candidiasis, trichomoniasis, or coinfection. A new molecular test assays the vaginal microbiome and organisms that cause three common infections. The objective of the trial was to evaluate the clinical accuracy of the investigational test for vaginal swabs collected by patients (self) or clinicians. The primary and secondary outcomes were to compare the investigational test with reference methods for the three most common causes of vaginitis and compare clinician-collected with self-collected swabs...
June 6, 2017: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28589198/excellent-peroxidase-mimicking-property-of-cuo-pt-nanocomposites-and-their-application-as-an-ascorbic-acid-sensor
#11
Xinhuan Wang, Qiusen Han, Shuangfei Cai, Tian Wang, Cui Qi, Rong Yang, Chen Wang
Due to low cost and high stability, the applications of inorganic nanomaterials as efficient alternatives to natural enzymes are drawing much attention. In this work, novel CuO/Pt nanocomposites with high peroxidase-like activity were designed and applied for the colorimetric detection of ascorbic acid (AA). The nanocomposites were prepared by decorating Pt NPs on the surface of CuO nanosheets, which displayed good uniformity and showed improved distribution and stability. The catalytic activity of the prepared CuO/Pt nanocomposites was tested against various chromogenic substrates in the presence of H2O2, which displayed efficient peroxidase-like activity and high catalytic stability against temperature...
June 7, 2017: Analyst
https://www.readbyqxmd.com/read/28581695/a-new-schiff-base-as-a-turn-off-fluorescent-sensor-for-cu-2-and-its-photophysical-properties
#12
Pritam Torawane, Suban K Sahoo, Amulrao Borse, Anil Kuwar
A new Schiff base receptor 1 was synthesized and its photophysical properties were investigated by absorption, emission and excitation techniques. Furthermore, its chromogenic and fluorogenic sensing abilities towards various metal ions were examined. Receptor 1 selectively detects Cu(2+) ion through fluorescence quenching and detection was not inhibited in the presence of other metal ions. From fluorescence titration, the limit of detection of receptor 1 as a fluorescent 'turn-off' sensor for the analysis of Cu(2+) was estimated to be 0...
June 5, 2017: Luminescence: the Journal of Biological and Chemical Luminescence
https://www.readbyqxmd.com/read/28567242/design-principles-of-spectroscopic-probes-for-biological-applications
#13
REVIEW
Jin Zhou, Huimin Ma
Spectroscopic (chromogenic, fluorescent, or chemiluminescent) probes have been widely used in many fields due to their high sensitivity and unrivaled spatiotemporal resolution. This area is an old one but always full of activity, because the rapid development of science and technology requires not only new probes for specific purposes (e.g., subcellular imaging) but also the update of current probes with more satisfactory properties. Based on our experiences and including existing knowledge, in this mini-review we briefly discuss the design strategies, response modes, and bioapplications of small molecular spectroscopic probes, in particular their advantages and disadvantages as well as possible research trends, which may be helpful to those who are interested in this continually growing research area...
October 1, 2016: Chemical Science
https://www.readbyqxmd.com/read/28559163/a-cost-saving-strategy-for-processing-isolated-uropathogens-in-community-acquired-urinary-tract-infections
#14
Juan Manuel Sánchez-Calvo, José Luís de Francisco, Eva Torres-Martos, Juan Carlos Alados Arboledas, Maria Dolores López Prieto
Outpatient urine samples are among the most commonly processed in a microbiology laboratory, which involves a high economic burden. The aim of this study was compare cost and efficiency to process uropathogens between MicroScan system (2010-2011) versus a chromogenic medium and the disk diffusion method (2013-2014). In the first period, a total 9918 bacterial populations were isolated from urine samples. Annual estimated costs during 2010 and 2011 for processing were EUR 53,818 and EUR 57,306, respectively (EUR 111,124 total)...
May 27, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28554670/serine-protease-isoforms-in-gloydius-intermedius-venom-full-sequences-molecular-phylogeny-and-evolutionary-implications
#15
Zhang-Min Yang, Hui Yu, Zhen-Zhen Liu, Jian-Zhu Pei, Yu-E Yang, Su-Xian Yan, Cui Zhang, Wen-Long Zhao, Zhe-Zhi Wang, Ying-Ming Wang, Inn-Ho Tsai
Nine distinct venom serine proteases (vSPs) of Gloydius intermedius were studied by transcriptomic, sub-proteomic and phylogenetic analyses. Their complete amino acid sequences were deduced after Expression Sequence Tag (EST) analyses followed by cDNA cloning and sequencing. These vSPs appear to be paralogs and contain the catalytic triads and 1-4 potential N-glycosylation sites. Their relative expression levels evaluated by qPCR were grossly consistent with their EST hit-numbers. The major vSPs were purified by HPLC and their N-terminal sequences matched well to the deduced sequences, while fragments of the minor vSPs were detected by LC-MS/MS identification...
May 26, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28553936/microfluidics-for-rapid-cytokeratin-immunohistochemical-staining-in-frozen-sections
#16
Saska Brajkovic, Diego G Dupouy, Laurence de Leval, Martin Am Gijs
Frozen sections (FS) of tumor samples represent a cornerstone of pathological intraoperative consultation and have an important role in the microscopic analysis of specimens during surgery. So far, immunohistochemical (IHC) stainings on FS have been demonstrated for a few markers using manual methods. Microfluidic technologies have proven to bring substantial improvement in many fields of diagnostics, though only a few microfluidic devices have been designed to improve the performance of IHC assays. In this work, we show optimization of a complete pan-cytokeratin chromogenic immunostaining protocol on FS using a microfluidic tissue processor into a protocol taking <12 min...
May 29, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28553935/multiplexed-ion-beam-imaging-analysis-for-quantitation-of-protein-expresssion-in-cancer-tissue-sections
#17
Sandra Rost, Jennifer Giltnane, Jennifer M Bordeaux, Chuck Hitzman, Hartmut Koeppen, Scot D Liu
Part of developing therapeutics is the need to identify patients who will respond to treatment. For HER2-targeted therapies, such as trastuzumab, the expression level of HER2 is used to identify patients likely to receive benefit from therapy. Currently, chromogenic immunohistochemistry on patient tumor tissue is one of the methodologies used to assess the expression level of HER2 to determine eligibility for trastuzumab. However, chromogenic staining is fraught with serious drawbacks that influence scoring, which is additionally flawed due to the subjective nature of human/pathologist bias...
May 29, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28549606/variable-performance-of-four-commercial-chromogenic-media-for-detection-of-methicillin-resistant-staphylococcus-aureus-isolates-harbouring-mecc
#18
Céline Dupieux, Camille Kolenda, Anders Rhod Larsen, Bruno Pichon, Mark Holmes, Michèle Bes, Christopher Teale, Elizabeth Dickson, Robert Hill, Robert Skov, Angela Kearns, Frédéric Laurent
In this study, the performances of four methicillin-resistant Staphylococcus aureus (MRSA) chromogenic screening media were compared for detecting mecC-positive MRSA. Using 111 clinical isolates representative of the various mecC-MRSA clones in Europe, chromID(®) MRSA (bioMérieux) and Brilliance(TM) MRSA 2 (Oxoid Ltd.) showed higher sensitivity (99.1% and 97.3%, respectively) than BBL(TM) CHROMagar(®) MRSA II (BD Diagnostics) and MRSASelect(TM) (Bio-Rad) (79.3% and 63.1%, respectively) (P < 0.0001). These findings have important implications for effective public health diagnostics and epidemiological surveillance of MRSA...
May 23, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28549468/microrna-608-inhibits-proliferation-of-bladder-cancer-via-akt-foxo3a-signaling-pathway
#19
Zhen Liang, Xiao Wang, Xin Xu, Bo Xie, Alin Ji, Shuai Meng, Shiqi Li, Yi Zhu, Jian Wu, Zhenghui Hu, Yiwei Lin, Xiangyi Zheng, Liping Xie, Ben Liu
BACKGROUND: Current evidence indicates that miR-608 is widely down-regulated in various malignant tumors including liver cancer, colon cancer, lung cancer and glioma, and acts as a tumor suppressor by inhibiting cell proliferation, invasion and migration or by promoting apoptosis. The specific biological function of miR-608 in bladder cancer is still unknown. METHODS: qRT-PCR and Chromogenic in Situ Hybridization (CISH) was conducted to assess the expression of miR-608 in paired BCa tissues and adjacent non-tumor bladder urothelial tissues...
May 26, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28547925/blockade-of-the-dissociation-of-nmmhc-iia-from-tnfr2-by-d39-a-saponin-from-liriope-muscari-suppresses-tissue-factor-expression-and-venous-thrombosis
#20
Ke-Feng Zhai, Jin-Rong Zheng, You-Mei Tang, Fang Li, Yan-Ni Lv, Yuan-Yuan Zhang, Zhen Gao, Jin Qi, Bo-Yang Yu, Jun-Ping Kou
BACKGROUND AND PURPOSE: Non-muscular myosin heavy chain IIA (NMMHC IIA) has been demonstrated to play a key role in tissue factor (TF) expression and venous thrombosis. However, whether natural products could inhibit thrombosis via NMMHC IIA remains unclear. In this study, we identified that a representative natural saponin D39 from Liriope muscari showed anti-thrombotic activity by targeting NMMHC IIA. EXPERIMENTAL APPROACH: TF expression and activity in endothelial cells were analysed in vitro by western blot and simplified chromogenic assays...
May 26, 2017: British Journal of Pharmacology
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