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https://www.readbyqxmd.com/read/29782688/oral-yeast-colonization-in-patients-with-primary-and-secondary-sj%C3%A3-gren-s-syndrome
#1
Clarissa Castro Galvão Medeiros, Luiz Gustavo Dos Anjos Borges, Karen Cherubini, Fernanda Gonçalves Salum, Renata Medina da Silva, Maria Antonia Zancanaro de Figueiredo
OBJECTIVE: The study aimed to investigate the pattern of oral yeast colonization of Sjögren's syndrome patients and its correlation to salivary flow rates, age and time of the disease progression. MATERIALS AND METHODS: Saliva and swab specimens were obtained from 45 patients (primary Sjögren's syndrome= 15/ secondary Sjögren's syndrome= 15/ healthy controls= 15). Yeast species were identified using culture method through chromogenic medium followed by polymerase chain reaction and Sanger sequencing...
May 21, 2018: Oral Diseases
https://www.readbyqxmd.com/read/29778469/communications-of-staphylococcus-aureus-and-non-aureus-staphylococcus-species-from-bovine-intramammary-infections-and-teat-apex-colonization
#2
Yasser S Mahmmod, Ilka Christine Klaas, Line Svennesen, Karl Pedersen, Hanne Ingmer
The role of non-aureus staphylococci (NAS) in the risk of acquisition of intramammary infections with Staphylococcus aureus is vague and still under debate. The objectives of this study were to (1) investigate the distribution patterns of NAS species from milk and teat skin in dairy herds with automatic milking systems, and (2) examine if the isolated NAS influences the expression of S. aureus virulence factors controlled by the accessory gene regulator (agr) quorum sensing system. In 8 herds, 14 to 20 cows with elevated somatic cell count were randomly selected for teat skin swabbing and aseptic quarter foremilk samples from right hind and left front quarters...
May 16, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29778192/genomic-characterization-of-coagulase-negative-staphylococci-including-methicillin-resistant-staphylococcus-sciuri-causing-bovine-mastitis
#3
Manouchehr Khazandi, Abd Al-Bar Al-Farha, Geoffrey W Coombs, Mark O'Dea, Stanley Pang, Darren J Trott, Ricardo R Aviles, Farhid Hemmatzadeh, Henrietta Venter, Abiodun D Ogunniyi, Andrew Hoare, Sam Abraham, Kiro R Petrovski
Methicillin-resistant coagulase-negative staphylococci (MRCoNS) have recently emerged as a significant cause of bovine mastitis worldwide. Here we describe the isolation of MRCoNS from cases of bovine mastitis from a single dairy farm in Australia. Fourteen CoNS isolates were identified as MRCoNS on the basis of having an oxacillin MIC of ≥0.5 μg/mL. The isolates were speciated as S. chromogenes (n = 1) S. fleurettii (n = 1), S. haemolyticus (n = 2), S. sciuri (n = 5), S. simulans (n = 1) S...
June 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29775069/simultaneous-release-and-labelling-of-o-and-n-glycans-allowing-for-rapid-glycomic-analysis-by-online-lc-uv-esi-ms-ms
#4
Chengjian Wang, Yu Lu, Jianli Han, Wanjun Jin, Lingmei Li, Ying Zhang, Xuezheng Song, Linjuan Huang, Zhongfu Wang
Most glycoproteins and biological protein samples undergo both O- and N-glycosylation, making characterization of their structures very complicated and time-consuming. Nevertheless, to fully understand the biological functions of glycosylation, both the glycosylation forms need to be analyzed. Herein we report a versatile, convenient one-pot method in which O- and N-glycans are simultaneously released from glycoproteins and chromogenically labelled in situ and thus available for further characterization. In this procedure, glycoproteins are incubated with 1-phenyl-3-methyl-5-pyrazolone (PMP) in aqueous ammonium hydroxide, making O-glycans released from protein backbones by beta-elimination and N-glycans liberated by alkaline hydrolysis...
May 18, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29770859/ionic-liquid-coated-iron-nanoparticles-are-promising-peroxidase-mimics-for-optical-determination-of-h-2-o-2
#5
Faiza Zarif, Sajid Rauf, Muhammad Zahid Qureshi, Noor Samad Shah, Akhtar Hayat, Nawshad Muhammad, Abdur Rahim, Mian Hasnain Nawaz, Muhammad Nasir
Ionic liquid coated nanoparticles (IL-NPs) consisting of zero-valent iron are shown to display intrinsic peroxidase-like activity with enhanced potential to catalyze the oxidation of the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of hydrogen peroxide. This results in the formation of a blue green colored product that can be detected with bare eyes and quantified by photometry at 652 nm. The IL-NPs were further doped with bismuth to enhance its catalytic properties. The Bi-doped IL-NPs were characterized by FTIR, X-ray diffraction and scanning electron microscopy...
May 16, 2018: Mikrochimica Acta
https://www.readbyqxmd.com/read/29759198/colorimetric-determination-of-uranyl-uo-2-2-in-seawater-via-dnazyme-modulated-photosensitization
#6
Chengpeng Huang, Xiaoya Fan, Qiaomei Yuan, Xinfeng Zhang, Xiandeng Hou, Peng Wu
Various DNA-modulated photosensitization schemes have been reported for various applications, but DNAzyme has not yet been included. Here, we found the DNAzyme with metal ion as cofactor could also be efficient in modulation of photosensitization process. The UO2 2+ -specific DNAzyme can switch between double strand DNA (dsDNA) and single strand DNA (ssDNA) upon UO2 2+ cleavage, which can regulate the photosensitization process of SYBR Green I. Coupling with photosensitization-induced chromogenic reaction (TMB oxidation), a simple colorimetric sensor for UO2 2+ was developed accordingly...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759183/evaluation-of-thrombin-inhibitory-activity-of-catechins-by-online-capillary-electrophoresis-based-immobilized-enzyme-microreactor-and-molecular-docking
#7
Qiao-Qiao Li, Feng-Qing Yang, Yin-Zhen Wang, Zhao-Yu Wu, Zhi-Ning Xia, Hua Chen
An online capillary electrophoresis (CE)-based thrombin (THR) immobilized enzyme microreactor (IMER) method was established to screen THR inhibitors in this study. S-2366 was used as chromogenic substrate for determination of THR activity and other kinetic constants. After continuously run for 50 times, the prepared IMER could still remain 89% of the initial immobilized enzyme activity. The Michaelis-Menten constant (Km ) of immobilized THR was measured as 0.514 mmol/L and the half-maximal inhibitory concentration (IC50 ) and inhibition constant (Ki ) of argatroban on THR were determined as 78...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29747777/mir-429-expression-in-bladder-cancer-and-its-correlation-with-tumor-behavior-and-clinical-outcome
#8
Chia-Lun Wu, Jar-Yi Ho, Shun-Hsing Hung, Dah-Shyong Yu
We previously showed that microRNA-429 (miR-429) played an important role in epithelial-mesenchymal transition (EMT) of urothelial cell carcinoma of the bladder. We herein evaluated the expression of miR-429 in bladder cancer and its potential relevance to clinicopathological characteristics and patient survival. Relative expression levels of miR-429 in surgical bladder cancer tissue specimens obtained from 76 patients with bladder cancer were measured by chromogenic in situ hybridization. miR-429 expression was significantly higher in specimens from alive patients than expired patients in both of 5-year overall survival (OS) (0...
June 2018: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/29741261/new-challenges-and-best-practices-for-the-laboratory-monitoring-of-factor-viii-and-factor-ix-replacement
#9
REVIEW
D Van den Bossche, K Peerlinck, M Jacquemin
Several recombinant factor VIII and factor IX concentrates with extended half-life (EHL) have recently been validated by clinical studies. The availability of these novel concentrates is expected to significantly facilitate the treatment of patients with hemophilia A and B. However, the modification applied to these molecules has introduced variations in their activity measurement in routine coagulation assays. Depending on the assays, underestimations of up to 10-fold or overestimations of up to approximately 30-fold in the measurements of the recovery have been reported in some factor concentrates...
May 2018: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/29737537/genetic-polymorphisms-in-the-fvii-gene-is-associated-with-lower-extremity-deep-venous-thrombosis-a-case-control-study
#10
Jian-Wei Liu, Dong-Qiong Chen
This study aims to explore the associations between FVII gene polymorphisms (R353Q, 5'F7, and -402G/A) and lower extremity deep venous thrombosis (LEDVT) in a Chinese Han population. LEDVT patients (153) and healthy people (174) were, respectively, as case and control groups and evaluated related biochemical indicators. Gene polymorphisms of R353Q, 5'F7, and -402G/A of FVII, serum FVII level, antithrombin activity, plasma fibrinogen content, and plasma D-dimer (D-D) level were determined by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), ELISA, chromogenic substrate assay, coagulating assay, and Immunoturbidimetry assay, respectively...
May 8, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29731265/mir-21-up-regulation-in-ampullary-adenocarcinoma-and-its-pre-invasive-lesions
#11
Deborah Saraggi, Francesca Galuppini, Giuseppe N Fanelli, Andrea Remo, Emanuele D L Urso, Ricardo Q Bao, Deborah Bacchin, Vincenza Guzzardo, Claudio Luchini, Chiara Braconi, Fabio Farinati, Massimo Rugge, Matteo Fassan
Poor information is available on the molecular landscape characterizing the carcinogenetic process leading to ampullary carcinoma. MiR-21 is one of the most frequently up-regulated miRNAs in pancreatic adenocarcinoma, a tumor sharing similar molecular features with ampullary adenocarcinomas (AVCs), above all with the pancreatic-biliary type. We profiled, by in situ hybridization (ISH), miR-21 expression in a series of 26 AVCs, 50 ampullary dysplastic lesions (35 low-grade [LG-IEN] and 15 high-grade [HG-IEN]) and 10 normal duodenal mucosa samples...
May 1, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29730973/development-of-in-situ-hybridization-for-detection-of-elephant-endotheliotropic-herpesvirus-in-asian-elephants
#12
Varankpicha Kochakul, Kittikorn Boonsri, Saruda Tiwananthagorn, Chalermchart Somgird, Chatchote Thitaram, Kidsadagon Pringproa
Elephant endotheliotropic herpesvirus (EEHV) is one of the most important viral infectious diseases affecting the elephant population worldwide, especially juveniles and young adults. We developed a chromogenic in situ hybridization (ISH) test for detection of EEHV in Asian elephants ( Elephas maximus). Digoxigenin (DIG) DNA probes from the polymerase and terminase genes of EEHV were synthesized using a PCR DIG-labeling method, and detection of hybridized probe to target EEHV DNA was carried out by anti-DIG immunolabeling...
May 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29720195/characterization-of-plasmodium-relictum-a-cosmopolitan-agent-of-avian-malaria
#13
Gediminas Valkiūnas, Mikas Ilgūnas, Dovilė Bukauskaitė, Karin Fragner, Herbert Weissenböck, Carter T Atkinson, Tatjana A Iezhova
BACKGROUND: Microscopic research has shown that Plasmodium relictum is the most common agent of avian malaria. Recent molecular studies confirmed this conclusion and identified several mtDNA lineages, suggesting the existence of significant intra-species genetic variation or cryptic speciation. Most identified lineages have a broad range of hosts and geographical distribution. Here, a rare new lineage of P. relictum was reported and information about biological characters of different lineages of this pathogen was reviewed, suggesting issues for future research...
May 2, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29713208/educational-needs-of-hematologists-and-laboratory-professionals-regarding-factor-activity-assays
#14
Dorothy M Adcock, Mazi Rasulnia, Natalia Holot, David L Cooper
Introduction: Diagnosis and management of hemophilia require accurate and precise measurements of factor activity levels. Activity is traditionally measured via one-stage (OS) clot-based assay; however, chromogenic substrate (CS) assays may be needed for certain cases. A survey was performed to understand assay-related knowledge gaps among hematologists and laboratory professionals. Methods: Separate web-based surveys were administered to hematologists who manage hemophilia and to laboratory professionals and queried practice patterns, knowledge of/attitudes toward CS assays, and interest in continuing education...
2018: Journal of Blood Medicine
https://www.readbyqxmd.com/read/29709451/development-of-an-enzymatic-assay-to-measure-lactate-in-perchloric-acid-precipitated-cerebrospinal-fluid
#15
Jun Lu, Jonathan R Genzen, David G Grenache
BACKGROUND: Individuals with inherited deficiencies of the pyruvate dehydrogenase complex or the respiratory chain complex can have increased concentrations of cerebrospinal fluid (CSF) lactate. Such measurements are clinical useful when measured in conjunction with pyruvate in order to calculate the lactate:pyruvate (L:P) ratio, a useful surrogate of cytosolic redox status. CSF pyruvate is measured in a protein-free supernatant prepared by the addition of CSF to perchloric acid while lactate is measured in untreated CSF...
April 27, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29705416/use-of-maldi-tof-to-characterize-staphylococcal-intramammary-infections-in-dairy-goats
#16
Véronique Bernier Gosselin, Jessica Lovstad, Simon Dufour, Pamela R F Adkins, John R Middleton
The most common pathogens causing intramammary infections (IMI) in dairy goats are staphylococci. Gene sequencing has been the reference method for identification of staphylococcal species, but MALDI-TOF mass spectrometry could represent a rapid and cost-effective alternative method. The objectives were to evaluate the typeability and accuracy of partial gene sequencing and MALDI-TOF for identifying staphylococci isolated from caprine milk samples, and to evaluate the relationship between staphylococcal species IMI, milk somatic cell score (SCS), and milk yield (MY)...
April 25, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29700691/-nano-immuno-test-for-the-detection-of-live-mycobacterium-avium-subspecies-paratuberculosis-bacilli-in-the-milk-samples-using-magnetic-nano-particles-and-chromogen
#17
Manju Singh, Shoor Vir Singh, Saurabh Gupta, Kundan Kumar Chaubey, Bjorn John Stephan, Jagdip Singh Sohal, Manali Dutta
Early rapid detection of Mycobacterium avium subspecies paratuberculosis (MAP) bacilli in milk samples is the major challenge since traditional culture method is time consuming and laboratory dependent. We report a simple, sensitive and specific nano-technology based 'Nano-immuno test' capable of detecting viable MAP bacilli in the milk samples within 10 h. Viable MAP bacilli were captured by MAP specific antibody-conjugated magnetic nano-particles using resazurin dye as chromogen. Test was optimized using true culture positive (10-bovine and 12-goats) and true culture negative (16-bovine and 25-goats) raw milk samples...
April 26, 2018: Veterinary Research Communications
https://www.readbyqxmd.com/read/29685347/optical-method-for-detecting-oxygen-via-the-chromogenic-reaction-catalyzed-by-polyphenol-oxidase
#18
Abdelaziz Bouchaib, Abdellatif Ben Abdellah, Mohamed Bakkali, Amine Laglaoui, Abdelhay Arakrak, Carlo Carboni, Abdelaziz Benjouad, Amal Maurady
The present work describes a method for detecting the ingress of gas phase oxygen into packed food. It uses the enzyme polyphenol oxidase (PPO)from Mushroom and Mediterranean dwarf palm. The PPO is incorporated into an indubiose film along with a non-toxic polyphenol such as gallic acid or chlorogenic acid. If exposed to oxygen, the test spot undergoes an irreversible and visible color change from pale to deep brown due to the PPO catalyzed oxidation of the respective polyphenol by oxygen. The color change can be detected visually or by spectrophotometry at 470 nm...
July 2018: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/29684365/leucocyte-dynamics-during-the-evolution-of-human-coronary-atherosclerosis-conclusions-from-a-7-fold-chromogen-based-immunohistochemical-evaluation
#19
Melissa Zuiderwijk, Marlieke Geerts, Connie Janssen van Rhijn, Antoon van den Bogaerdt, Jaap F Hamming, Rogier A van Dijk, Jan H Lindeman
Atherosclerosis is a complex process with strong inflammatory component. We developed a straightforward 7-fold staining protocol for simultaneous assessment of dominant leucocyte classes, vascularization, and expression of the putative foam cell maker CD36. The method was applied on human coronaries covering the full spectrum of atherosclerotic disease. Results confirm the progressive association of macrophages and T-cells with the process, and a global presence of mast cells. B-cells exclusively present in adventitial follicles that accompany the process plaque destabilization (thin cap and ruptured lesions) and are otherwise absent...
April 20, 2018: American Journal of Pathology
https://www.readbyqxmd.com/read/29683655/alkaline-phosphatase-assay-based-on-the-chromogenic-interaction-of-diethanolamine-with-4-aminophenol
#20
Jian Sun, Jiahui Zhao, Xingfu Bao, Qifeng Wang, Xiurong Yang
Diethanolamine (DEA) has been extensively utilized as an alkaline buffer in current assays of alkaline phosphatase (ALP) activity in the past decades. While playing the role of a buffer, DEA has been widely ignored about its chemical reactivity in such assays. Herein, we report an interesting chromogenic interaction between DEA and 4-aminophenol (AP) in the presence of H2 O for the first time, which inspires us to develop a novel DEA-participated ALP activity assay by using 4-aminophenyl phos-phate (APP) as a substrate...
April 23, 2018: Analytical Chemistry
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