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Meritxell Garcia-Quintanilla, Laurent Poirel, Patrice Nordmann
A novel chromogenic medium CHROMagar mSuperCARBA was evaluated to detect carbapenem-resistant Gram-negatives. This medium is as sensitive and as specific as the SUPERCARBA medium for detecting KPC, MBL and OXA-48-type producers (100% and 100%, respectively) and is compatible with subsequent testing of carbapenemase activity using the RAPIDEC(®) CARBA NP.
October 19, 2017: Diagnostic Microbiology and Infectious Disease
Yumiko Komori, Toru Hifumi, Akihiko Yamamoto, Atsushi Sakai, Manabu Ato, Kyoko Sawabe, Toshiaki Nikai
Rhabdophis lateralis, a colubrid snake distributed throughout the continent of Asia, has recently undergone taxonomic revisions. Previously, Rhabdophis lateralis was classified as a subspecies of R. tigrinus (Yamakagashi) until 2012, when several genetic differences were discovered which classified this snake as its own species. To elucidate the toxicity of venom from this poorly studied colubrid, various biological activities were compared between the venom from the two snake species. The components of their venom were compared by the elution profiles of reversed-phase HPLC and SDS-PAGE, and gel filtrated fractions were tested for effects on blood coagulation...
November 17, 2017: Toxins
Antonio Mancini, Chantal Di Segni, Carmine Bruno, Giulio Olivieri, Francesco Guidi, Andrea Silvestrini, Elisabetta Meucci, Patrick Orlando, Sonia Silvestri, Luca Tiano, Alfredo Pontecorvi
BACKGROUND AND AIMS: Growth hormone deficiency (GHD) is a condition associated with increased cardiovascular risk and insulin-resistance. Oxidative stress (OS) could be a mechanism underlying both these phenomena. In order to investigate plasma antioxidant defenses in such condition, we evaluated adults with GHD, compared with controls and metabolic syndrome patients (MetS), studying plasma total antioxidant capacity (TAC) and coenzyme Q10 (CoQ10, lipophilic antioxidant) levels, both in its oxidized and reduced forms, correlating this data with metabolic and hormonal pattern...
November 15, 2017: Endocrine
Sami Blom, Lassi Paavolainen, Dmitrii Bychkov, Riku Turkki, Petra Mäki-Teeri, Annabrita Hemmes, Katja Välimäki, Johan Lundin, Olli Kallioniemi, Teijo Pellinen
The paradigm of molecular histopathology is shifting from a single-marker immunohistochemistry towards multiplexed detection of markers to better understand the complex pathological processes. However, there are no systems allowing multiplexed IHC (mIHC) with high-resolution whole-slide tissue imaging and analysis, yet providing feasible throughput for routine use. We present an mIHC platform combining fluorescent and chromogenic staining with automated whole-slide imaging and integrated whole-slide image analysis, enabling simultaneous detection of six protein markers and nuclei, and automatic quantification and classification of hundreds of thousands of cells in situ in formalin-fixed paraffin-embedded tissues...
November 14, 2017: Scientific Reports
Natalia González, Marcos Grünhut, Ivana Šrámková, Adriana G Lista, Burkhard Horstkotte, Petr Solich, Hana Sklenářová, Carolina C Acebal
A fully automated spectrophotometric method based on flow-batch analysis has been developed for the determination of clenbuterol including an on-line solid phase extraction using a molecularly imprinted polymer (MIP) as the sorbent. The molecularly imprinted solid phase extraction (MISPE) procedure allowed analyte extraction from complex matrices at low concentration levels and with high selectivity towards the analyte. The MISPE procedure was performed using a commercial MIP cartridge that was introduced into a guard column holder and integrated in the analyzer system...
February 1, 2018: Talanta
Simona Stolnicu, Iulia Barsan, Lien Hoang, Prusha Patel, Cristina Terinte, Anna Pesci, Sarit Aviel-Ronen, Takako Kiyokawa, Isabel Alvarado-Cabrero, Malcolm C Pike, Esther Oliva, Kay J Park, Robert A Soslow
We sought to classify endocervical adenocarcinomas (ECAs) based on morphologic features linked to etiology (ie, human papillomavirus [HPV] infection), unlike the World Health Organization 2014 classification. The International Endocervical Adenocarcinoma Criteria and Classification (IECC criteria), described herein, distinguishes between human papillomavirus-associated adenocarcinoma (HPVA), recognized by the presence of luminal mitoses and apoptosis seen at scanning magnification, and no or limited HPVA features (nonhuman papillomavirus-associated adenocarcinoma [NHPVA])...
November 9, 2017: American Journal of Surgical Pathology
Kamil Strzelak, Natalia Rybkowska, Agnieszka Wiśniewska, Robert Koncki
The Multicommutated Flow Analysis (MCFA) system for the estimation of clinical iron parameters: Serum Iron (SI), Unsaturated Iron Binding Capacity (UIBC) and Total Iron Binding Capacity (TIBC) has been proposed. The developed MCFA system based on simple photometric detection of iron with chromogenic agent (ferrozine) enables a speciation of transferrin (determination of free and Fe-bound protein) in human serum. The construction of manifold was adapted to the requirements of measurements under changing conditions...
December 1, 2017: Analytica Chimica Acta
Ajcharapan Tantipanjaporn, Samran Prabpai, Kanoknetr Suksen, Palangpon Kongsaeree
A rhodamine-thiourea conjugate RTP with an o-phenylenediamine linker was developed as a fluorogenic chemodosimeter for Hg(2+) detection. In the presence of Hg(2+), a colorless solution of RTP turned pink with a maximum absorption band at 555nm and with a 62-fold fluorescence enhancement at 578nm (Φ=0.34). RTP is highly selective to Hg(2+) among other metal ions with a detection limit of 1.6nM (0.3ppb). A similar rhodamine analog with a flexible ethylenediamine spacer was less selective and less sensitive than RTP...
October 25, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Nadezhda S Yurova, Alexandra Danchuk, Sarah N Mobarez, Nongnoot Wongkaew, Tatiana Rusanova, Antje J Baeumner, Axel Duerkop
Electrospun nanofibers (ENFs) are promising materials for rapid diagnostic tests like lateral flow assays and dipsticks because they offer an immense surface area while excluding minimal volume, a variety of functional surface groups, and can entrap functional additives within their interior. Here, we show that ENFs on sample pads are superior in comparison to standard polymer membranes for the optical detection of biogenic amines (BAs) in food using a dipstick format. Specifically, cellulose acetate (CA) fibers doped with 2 mg/mL of the chromogenic and fluorogenic amine-reactive chameleon dye Py-1 were electrospun into uniform anionic mats...
November 7, 2017: Analytical and Bioanalytical Chemistry
Xiaonan Zhang, Lei Yue, Zhanqing Zhang, Zhenghong Yuan
While chronic hepatitis B remains a global public health problem, the detailed spatiotemporal dynamics of the key molecular events leading to the multiplication and egress of hepatitis B virus (HBV) are still largely unclear. Previously, we developed a chromogenic in situ hybridization assay for detection of HBV RNA, DNA and covalently closed circular DNA in clinical liver biopsies. Here, we report the establishment of a fluorescent in situ hybridization method for the visualization of HBV RNA, HBV core particle DNA and intranuclear DNA in a tetracycline-inducible HBV replication system (HepAD38) and a de novo infection system (HepG2-NTCP)...
November 8, 2017: Emerging Microbes & Infections
Salvatore Sortino, Federica Sodano, Elena Gazzano, Aurore Fraix, Barbara Rolando, Loretta Lazzarato, Marina Russo, Marco Blangetti, Chiara Riganti, Roberta Fruttero, Alberto Gasco
The design, synthesis, spectroscopic and photochemical properties and biological evaluation of a novel, molecular hybrid that is able to deliver nitric oxide (NO) into the mitochondria are reported. This molecular conjugate unites a tailored o-CF3-p-nitroaniline chromophore, for photo-regulated NO release, and a Rhodamine moiety, for mitochondria targeting, in the same molecular skeleton via an alkyl spacer. A combination of steady-state and time-resolved spectroscopic and photochemical experiments demonstrate that the two chromogenic units preserve their individual photophysical and photochemical properties in the conjugate quite well...
November 7, 2017: ChemMedChem
Victoria J Laye, Ram Karan, Jong-Myoung Kim, Wolf T Pecher, Priya DasSarma, Shiladitya DasSarma
The Antarctic microorganism Halorubrum lacusprofundi harbors a model polyextremophilic β-galactosidase that functions in cold, hypersaline conditions. Six amino acid residues potentially important for cold activity were identified by comparative genomics and substituted with evolutionarily conserved residues (N251D, A263S, I299L, F387L, I476V, and V482L) in closely related homologs from mesophilic haloarchaea. Using a homology model, four residues (N251, A263, I299, and F387) were located in the TIM barrel around the active site in domain A, and two residues (I476 and V482) were within coiled or β-sheet regions in domain B distant to the active site...
November 6, 2017: Proceedings of the National Academy of Sciences of the United States of America
Keita Osada, Tatsuro Minami, Takashi Arioka, Takumi Sakai, Shunsuke Tawara, Koh Kawasaki, Jawed Fareed, Osamu Matsuzaki
INTRODUCTION: Extracellular histones are reported to increase thrombin generation in the plasma and induce endothelial cell death in vitro. These effects of histones were suggested to involve histone-induced inhibition of TM-dependent activated protein C (APC) generation. Therefore, we hypothesized that TM alfa, a recombinant human soluble TM, attenuates these effects of histones by promoting the generation of APC. In the present study, we investigated the effects of TM alfa on the histone-induced decrease in APC generation, an increase in thrombin generation, and endothelial cell death in vitro...
October 27, 2017: Thrombosis Research
K Johani, D Abualsaud, D M Costa, H Hu, G Whiteley, A Deva, K Vickery
BACKGROUND: Organisms causing healthcare associated infections can be sourced from the inanimate environment around patients. Residing in a biofilm increases the chances of these organisms persisting in the environment. We aimed to characterise bacterial environmental contamination, genetically and physiologically, and relate this to general intensive care unit (ICU) cleanliness. METHODS: Cleanliness was determined by adenosine triphosphate (ATP) measurements of 95 high-touch objects...
October 30, 2017: Journal of Infection and Public Health
Shazia Mumtaz, Li-Sheng Wang, Syed Zajif Hussain, Muhammad Abdullah, Zille Huma, Zafar Iqbal, Brian Creran, Vincent M Rotello, Irshad Hussain
We report a simple and economical colorimetric bacterial sensing strategy with catalytic amplification using dopamine-capped iron oxide (Dop-Fe3O4) nanoparticles. These nanoparticles catalyse the oxidation of a chromogenic substrate in the presence of H2O2 into a green colored product. The catalytic activity of the nanoparticles is inhibited in the presence of bacteria, providing naked eye detection of bacteria at 10(4) cfu mL(-1) and by spectrophotometric detection down to 10(2) cfu mL(-1).
November 14, 2017: Chemical Communications: Chem Comm
Vinodkumar Kulangara, Neetha Nair, Asok Sivasailam, Suchithra Sasidharan, Justin Davis Kollannur, Radhika Syam
Dairy cows affected with subclinical mastitis can be sources of virulent, antimicrobial-resistant Staphylococci to humans because of the excretion of the bacteria through their milk. This study focussed on the phenotypic and genotypic antibiotic resistance patterns of Staphylococci isolated from dairy cows in early dry period. Among 96 isolates of Gram positive cocci from 157 cows, 76 were identified as Coagulase Negative Staphylococci and the remaining 20 were Staphylococcus aureus. Typical amplicons of coagulase gene were obtained for all 20 samples of S...
2017: PloS One
J M Zhu, Y Shi, X Q Zhu, Y Yang, F H Jiang, C J Sun, W H Zhao, X T Han
Real-time detection of phosphate has significant meaning in marine environmental monitoring and forecasting the occurrence of harmful algal blooms. Conventional monitoring instruments are dependent on artificial sampling and laboratory analysis. They have various shortcomings for real-time applications because of the large equipment size and high production cost, with low target selectivity and the requirement of time-consuming procedures to obtain the detection results. We propose an optofluidic miniaturized analysis chip combined with micro-resonators to achieve real-time phosphate detection...
November 1, 2017: Lab on a Chip
M Muniesa, E Ballesté, L Imamovic, M Pascual-Benito, D Toribio-Avedillo, F Lucena, A R Blanch, J Jofre
The use of somatic coliphages as indicators of fecal and viral pollution in water and food has great potential due to the reliability, reproducibility, speed and cost effectiveness of methods for their detection. Indeed, several countries already use this approach in their water management policies. Although standardized protocols for somatic coliphage detection are available, user-friendly commercial kits would facilitate their routine implementation in laboratories. The new method presented here allows detection of up to 1 somatic coliphage in under 3...
October 16, 2017: Water Research
H Ciesielczuk, L M Phee, H Dolphin, M Wilks, B P Cherian, D W Wareham
BACKGROUND: Successful laboratory detection of carbapenemase-producing Enterobacteriaceae (CPE) in patient surveillance samples is a diagnostic challenge. In the absence of a gold standard for screening rectal swabs for CPE many phenotypic, genotypic, culture and non-culture based assays have been proposed for identifying these bacteria. AIM: To develop and optimise a CPE screening protocol capable of identifying all commonly encountered CPE, including those producing OXA-48-like carbapenemases...
October 23, 2017: Journal of Hospital Infection
Muhammad Saleem, Muhammad Rafiq, Muhammad Hanif, Muhammad Ashraf Shaheen, Sung-Yum Seo
The design and development of the fluorescence reporting molecules for the trace recognition of the metallic ions in the aqueous, mixed organic aqueous media, environmental specimen, living things e.g., body fluids, serum, urine etc is the evergreen research area. Among several methodologies utilized for these purposes, the fluorescent techniques have wonderful impact in the era of metal sensor development due to their acclamatory features of non-invasive detection process and appreciable sensitivity. The utilization of small organic molecules for the sensation of metallic ions through the fluorescent techniques harvested the wonderful results having some interesting features of chromogenic response on metal binding, robustness in the preparation and applicability to establish the metallic accumulation level inside the live cells via bioimaging studies...
October 24, 2017: Journal of Fluorescence
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