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https://www.readbyqxmd.com/read/28333325/triple-paternal-contribution-to-a-normal-complete-molar-chimeric-singleton-placenta
#1
I Ariel, D Goldman-Wohl, S Yagel, E Gazit, R Loewenthal
A comprehensive study of unusual cases of placental pathology may provide insight into mechanisms of normal human fertilization and early embryonic development by examining the exception to the rule. A gravida three para two 39-year-old woman was monitored by ultrasound from 16 weeks of gestation for cystic placenta. A female newborn was born at 36 weeks gestation. Pathologic examination of the partially cystic placenta revealed a singleton placenta comprised of 2/3 normal placenta and 1/3 complete hydatidiform mole, largely degenerated...
March 14, 2017: Human Reproduction
https://www.readbyqxmd.com/read/28322799/novel-method-for-measurement-of-heparin-anticoagulant-activity-using-spr
#2
Jing Zhao, Xinyue Liu, Anju Malhotra, Quanhong Li, Fuming Zhang, Robert J Linhardt
A novel method has been developed for the easy measurement of heparin's anticoagulant activity using surface plasmon resonance. The anticoagulant activity of target heparin was evaluated by measuring the competitive antithrombin III binding of analyte heparin in the solution phase and USP heparin immobilized on chip surface. Heparins, obtained from different animal sources, and low molecular weight heparins were analyzed. The results were reproducible and correlated well with the results of chromogenic assays (correlation coefficient r = 0...
March 18, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/28320219/monitoring-of-argatroban-and-lepirudin
#3
Holger Seidel, Hans-Jürgen Kolde
Monitoring of direct thrombin inhibitors (DTIs) in patients with heparin-induced thrombocytopenia (HIT) is primarily performed using the activated partial thromboplastin time (aPTT). This assay is poorly standardized, reagent dependent, and not DTI specific. We compared aPTT, thrombin time (TT), and prothrombin time (PT) to drug levels obtained by the ecarin chromogenic assay (ECA). We analyzed 495 samples of patients with confirmed or suspected HIT on treatment with either argatroban (n = 37) or lepirudin (n = 80)...
January 1, 2017: Clinical and Applied Thrombosis/hemostasis
https://www.readbyqxmd.com/read/28318109/agreement-between-factor-xiii-activity-and-antigen-assays-in-measurement-of-factor-xiii-a-french-multicenter-study-of-147-human-plasma-samples
#4
C Caron, R Meley, V Le Cam Duchez, M F Aillaud, C Lavenu-Bombled, F Dutrillaux, C Flaujac, A Ryman, C Ternisien, D Lasne, H Galinat, C Pouplard
INTRODUCTION: Factor XIII (FXIII) deficiency is a rare hemorrhagic disorder whose early diagnosis is crucial for appropriate treatment and prophylactic supplementation in cases of severe deficiency. International guidelines recommend a quantitative FXIII activity assay as first-line screening test. FXIII antigen measurement may be performed to establish the subtype of FXIII deficiency (FXIIID) when activity is decreased. METHODS: The aim of this multicenter study was to evaluate the analytical and diagnostic levels of performance of a new latex immunoassay, K-Assay(®) FXIII reagent from Stago, for first-line measurement of FXIII antigen...
March 20, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28318106/lack-of-rivaroxaban-influence-on-a-prothrombinase-based-assay-for-the-detection-of-activated-c-protein-resistance-an-italian-ex-vivo-and-in-vitro-study-in-normal-subjects-and-factor-v-leiden-carriers
#5
G Gessoni, S Valverde, L Valle, F Gessoni, P Caruso, R Valle
INTRODUCTION: Activated protein C resistance (APCr) leads to hypercoagulability and is due, often but not exclusively, to Factor V Leiden (FVL). The aim of this study was to assess the ex vivo and in vitro interference of the direct factor Xa inhibitor rivaroxaban (RIV) on a prothrombinase-based assay for APCr detection. METHODS: An ex vivo study was performed on fresh plasma samples obtained from 44 subjects with FV wild-type and seven with FVL heterozygous, all treated with RIV...
March 20, 2017: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/28315656/expression-of-odc1-spd-spm-and-azin1-in-the-hypothalamus-ovary-and-uterus-during-rat-estrous-cycle
#6
Joseph R D Fernandes, Sammit Jain, Arnab Banerjee
The aim of the present study was to investigate variation in the expression pattern of ornithine decarboxylase (ODC1), spermine (SPM), spermidine (SPD) and antizyme inhibitor (AZIN1) in hypothalamus, ovary and uterus during the estrous cycle of rats. Further, to understand any correlation between polyamines and GnRH I expression in hypothalamus; effect of putrescine treatment on GnRH I expression in hypothalamus and progesterone and estradiol levels in serum were investigated. The study also aims in quantifying all the immunohistochemistry images obtained based on pixel counting algorithm to yield the relative pixel count...
March 15, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28315237/sits-derivatization-of-peptides-to-enhance-266%C3%A2-nm-ultraviolet-photodissociation-uvpd
#7
M Montana Quick, M Rachel Mehaffey, Robert W Johns, W Ryan Parker, Jennifer S Brodbelt
N-terminal derivatization of peptides with the chromogenic reagent 4-acetamido-4-isothiocyanatostilbene-2,2-disulfonic acid (SITS) is demonstrated to enhance the efficiency of 266 nm ultraviolet photodissociation (UVPD). Attachment of the chromophore results in a mass shift of 454 Da and provides significant gains in the number and abundances of diagnostic fragment ions upon UVPD. Activation of SITS-tagged peptides with 266 nm UVPD leads to many fragment ions akin to the a/b/y ions commonly produced by CID, along with other sequence ions (c, x, and z) typically accessed through higher energy pathways...
March 17, 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28314985/the-role-of-new-oral-anticoagulants-in-orthopaedics-an-update-of-recent-evidence
#8
REVIEW
Dimitrios V Papadopoulos, Ioannis Kostas-Agnantis, Ioannis Gkiatas, Andreas G Tsantes, Panagiota Ziara, Anastasios V Korompilias
Rivaroxaban, dabigatran, apixaban and edoxaban are the four available new oral anticoagulants (NOAC) which are currently approved for venous thromboembolism prophylaxis after total hip and knee replacement. Large phase 3 and phase 4 studies comparing NOAC with low molecular weight heparins have shown similar results regarding the efficacy and safety of these two categories of anticoagulants. Management of bleeding complications is a matter of great significance. Three reversal agents have been developed: idarucizumab, andexanet alfa and ciraparantag...
March 17, 2017: European Journal of Orthopaedic Surgery & Traumatology: Orthopédie Traumatologie
https://www.readbyqxmd.com/read/28302536/rna-chromogenic-in-situ-hybridization-assay-with-clinical-automated-platform-is-a-sensitive-method-in-detecting-high-risk-human-papillomavirus-in-squamous-cell-carcinoma
#9
Javier E Mendez-Pena, Peter M Sadow, Vania Nose, Mai P Hoang
Detection of active human papillomavirus (HPV) is clinically important, as its presence has been shown to correlate with favorable clinical outcomes and better response to treatment in oropharyngeal squamous cell carcinomas (SCC). Using a clinical automated platform, we compared the performance of commercially available HPV DNA and RNA in situ hybridization (ISH) probes in archival tissues of 57 SCC. Importantly, a clinical automated platform gives 1) consistent and reproducible results for HPV ISH and 2) better standardization across clinical laboratories...
March 13, 2017: Human Pathology
https://www.readbyqxmd.com/read/28297801/-effect-of-curcumin-on-intestinal-mucosal-mechanical-barrier-in-rats-with-non-alcoholic-fatty-liver-disease
#10
H T Hou, Y M Qiu, H W Zhao, D H Li, Y T Liu, Y Z Wang, S H Su
Objective: To investigate the effect of curcumin on intestinal mucosal mechanical barrier in rats with non-alcoholic fatty liver disease. Methods: A total of 30 male Sprague-Dawley rats were equally divided into normal control group, model group, and curcumin intervention group. The rats in the model group and the curcumin intervention group were given high-fat feed for 16 weeks, and those in the curcumin intervention group were given curcumin 200 mg/kg/day by gavage once a day after 8 weeks of high-fat feeding...
February 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28293887/protein-and-protease-sensing-by-allosteric-derepression
#11
Hui Chin Goh, Farid J Ghadessy, Saurabh Nirantar
Peptide motifs are crucial mediators of protein-protein interactions as well as sites of specific protease activity. The detection and characterization of these events is therefore indispensable for a detailed understanding of cellular regulation. Here, we present versatile and modular sensors that allow the user to detect protease activity and protein-peptide interactions, as well as to screen for inhibitors using chromogenic, fluorescent, or luminescent output.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28293235/distribution-of-cfr-in-staphylococcus-spp-and-escherichia-coli-strains-from-pig-farms-in-china-and-characterization-of-a-novel-cfr-carrying-f43-a-b-plasmid
#12
Xiao-Qin Liu, Jing Wang, Wei Li, Li-Qing Zhao, Yan Lu, Jian-Hua Liu, Zhen-Ling Zeng
The multi-resistance gene cfr is widely distributed among various gram-positive and gram-negative species in livestock in China. To better understand the epidemiology of cfr among Staphylococcus spp. and E. coli isolates, 254 Staphylococcus spp. and 398 E. coli strains collected from six swine farms in China were subjected to prevalence and genetic analysis. Forty (15.7%) Staphylococcus spp. isolates, including 38 Staphylococcus sciuri strains, one Staphylococcus chromogenes strain, and one Staphylococcus lentus strain, and two (0...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28291248/a-curated-collection-of-tissue-microarray-images-and-clinical-outcome-data-of-prostate-cancer-patients
#13
Qing Zhong, Tiannan Guo, Markus Rechsteiner, Jan H Rüschoff, Niels Rupp, Christian Fankhauser, Karim Saba, Ashkan Mortezavi, Cédric Poyet, Thomas Hermanns, Yi Zhu, Holger Moch, Ruedi Aebersold, Peter J Wild
Microscopy image data of human cancers provide detailed phenotypes of spatially and morphologically intact tissues at single-cell resolution, thus complementing large-scale molecular analyses, e.g., next generation sequencing or proteomic profiling. Here we describe a high-resolution tissue microarray (TMA) image dataset from a cohort of 71 prostate tissue samples, which was hybridized with bright-field dual colour chromogenic and silver in situ hybridization probes for the tumour suppressor gene PTEN. These tissue samples were digitized and supplemented with expert annotations, clinical information, statistical models of PTEN genetic status, and computer source codes...
March 14, 2017: Scientific Data
https://www.readbyqxmd.com/read/28279252/calibration-of-the-ph-eur-human-coagulation-factor-viii-concentrate-brp-batch-5
#14
S Raut, A Costanzo, A Daas, K H Buchheit
The European Pharmacopoeia Biological Reference Preparation (Ph. Eur. BRP) for Factor VIII Concentrate batch 5 was established through a collaborative study involving 14 laboratories organised by the European Directorate for the Quality of Medicines & HealthCare (EDQM, Council of Europe) to be used as working standard for potency determination of human coagulation Factor VIII (FVIII) preparations. The potency of the BRP batch 5 was assigned with reference to the WHO 8(th) International Standard (IS) for FVIII Concentrate and the BRP batch 4...
2017: Pharmeuropa Bio & Scientific Notes
https://www.readbyqxmd.com/read/28278112/purification-and-characterization-of-a-novel-trypsin-like-protease-from-green-seeded-chickpea-cicer-arientum
#15
Tooba Naz Shamsi, Romana Parveen, Priyankar Sen, Sadaf Fatima
The present study describes the purification and physicochemical and biochemical characterization of trypsin-like protease from green-seeded chickpea (Cicer arientum). The crude extract of chickpea trypsin (CpT) was obtained by homogenization followed by differential ammonium sulfate precipitation. The CpT was purified by ion-exchange chromatography on diethylaminoethyl (DEAE) column, pre-equilibrated with 20 mM tris-CaCl2 buffer (pH 8.2) with a flow rate of 0.5 mL min(-1). The molecular weight and purity of ∼23 kDa of CpT were determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 10, 2017: Preparative Biochemistry & Biotechnology
https://www.readbyqxmd.com/read/28276247/inducible-sequential-oxidation-process-in-water-soluble-copper-nanoclusters-for-direct-colorimetric-assay-of-hydrogen-peroxide-in-a-wide-dynamic-and-sampling-range
#16
Yibing Du, Jun Fang, Hongli Wang, Yang Yang
Direct and fast detection methods for H2O2 have great demand in materials science, biology, and medicine. Colorimetric assay of H2O2 has been regarded as one versatile approach that can avoid tedious operation and complicated setup. In this report, we provided a cost-effective and time-saving H2O2 colorimetric assay strategy based on a mercaptosuccinic acid (MSA)-stabilized Cu nanocluster (NC) probe without using any chromogenic reagent. Direct and fast colorimetric detection of H2O2 was realized based on the color change of MSA-capped Cu NCs in aqueous medium...
March 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28267763/molecular-characterization-of-vancomycin-resistant-enterococcus-faecium-isolates-from-bermuda
#17
Patrick Eberechi Akpaka, Shivnarine Kissoon, Clyde Wilson, Padman Jayaratne, Ashley Smith, George R Golding
Molecular characteristics of vancomycin resistant enterococci isolates from Bermuda Island is currently unknown. This study was conducted to investigate phenotypic and genotypic characteristics of VRE isolates from Bermuda Island using the chromogenic agar, E-tests, polymerase chain reaction (PCR), pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Eighteen E. faecium isolates were completely analyzed and were all resistant to vancomycin, susceptible to linezolid and quinupristin/dalfopristin, positive for vanA and esp genes...
2017: PloS One
https://www.readbyqxmd.com/read/28267570/identification-of-microorganisms-grown-on-chromogenic-media-by-maldi-tof-ms
#18
Petra Lüthje, Arthur B Pranada, Duncan Carruthers-Lay, Marc Desjardins, Olivier Gaillot, David Wareham, Holly Ciesielczuk, Volkan Özenci
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and chromogenic media are widely used in clinical microbiology laboratories to facilitate the rapid selection and identification of pathogens. The aim of this study was to evaluate whether usage of chromogenic media limits the diagnostic performance of MALDI-TOF MS for microbial identification. A total of 386 microorganisms collected and analyzed at five laboratories were included. Isolates were cultured on relevant chromogenic media and non-selective agar plates in parallel and identified using the Bruker MALDI-TOF MS...
March 4, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28266777/exploiting-sp2-hybridization-in-the-development-of-potent-1-5-a-l-arabinanase-inhibitors
#19
Travis Coyle, Aleksandra Debowski, Annabelle Varrot, Keith A Stubbs
The synthesis of potent inhibitors of GH93 arabinanases as well as a synthesis of a chromogenic substrate to measure GH93 arabinanase activity is described. An insight into the reasons behind the potency of the inhibitors was gained through X-ray crystallographic analysis using the arabinanase Arb93A from Fusarium graminearum. These compounds lay a foundation for future inhibitor development as well as for the use of the chromogenic substrate in biochemical studies of GH93 arabinanases.
March 7, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28264199/factor-activity-assays-for-monitoring-extended-half-life-fviii-and-factor-ix-replacement-therapies
#20
Steve Kitchen, Stefan Tiefenbacher, Robert Gosselin
The advent of modified factor VIII (FVIII) and factor IX (FIX) molecules with extended half-lives (EHLs) compared with native FVIII and FIX represents a major advance in the field of hemophilia care, with the potential to reduce the frequency of prophylactic injections and/or to increase the trough level prior to subsequent injections. Monitoring treatment through laboratory assays will be an important part of ensuring patient safety, including any tailoring of prophylaxis. Several approaches have been used to extend half-lives, including PEGylation, and fusion to albumin or immunoglobulin...
March 6, 2017: Seminars in Thrombosis and Hemostasis
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