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Renata Tabola, Magdalena Zaremba-Czogalla, Dagmara Baczynska, Roberto Cirocchi, Kamila Stach, Krzysztof Grabowski, Katarzyna Augoff
There are numerous reports suggesting that fibroblast activating protein-α (FAP-α) plays an important role in invasion of various tumor types. We studied the expression pattern of FAP-α in esophageal squamous cell carcinoma (ESCC) patients who had not been treated primarily and those who had received neoadjuvant radiochemotherapy. Our goal was to establish whether readily available tissue specimens fixed in formalin and stored in paraffin blocks for years might still be a source of FAP-α RNA for PCR analysis...
June 20, 2017: European Journal of Histochemistry: EJH
Georgia Vrioni, Vasiliki Mamali, Olympia Zarkotou, Evaggelia Voulgari, Evaggelia Dimitroulia, Katerina Themeli-Digalaki, Spyros Pournaras, Athanassios Tsakris
OBJECTIVES: To study the performance of the rapid chromogenic βLACTA test for the rapid detection of expanded-spectrum cephalosporin (ESC) nonsusceptibility among Enterobacteriaceae, in a region endemic for potent β-lactamases. METHODS: The βLACTA test was applied prospectively on 235 consecutive Enterobacteriaceae clinical isolates and163 previously characterized ESC-nonsusceptible Enterobacteriaceae producing a range of β-lactamases. RESULTS: βLACTA exhibited excellent sensitivity (96...
July 18, 2017: Journal of Global Antimicrobial Resistance
Jyun-Liang Lin, James M Wagner, Hal S Alper
Within the Design-Build-Test Cycle for strain engineering, rapid product detection and selection strategies remain challenging and limit overall throughput. Here we summarize a wide variety of modalities that transduce chemical concentrations into easily measured absorbance, luminescence, and fluorescence signals. Specifically, we cover protein-based biosensors (including transcription factors), nucleic acid-based biosensors, coupled enzyme reactions, bioorthogonal chemistry, and fluorescent and chromogenic dyes and substrates as modalities for detection...
July 16, 2017: Biotechnology Advances
Jianlei Shen, Binquan Luan, Hao Pei, Zaixing Yang, Xiaolei Zuo, Gang Liu, Jiye Shi, Lihua Wang, Ruhong Zhou, Wenlong Cheng, Chunhai Fan
2D materials possess many interesting properties, and have shown great application potentials. In this work, the development of humidity-responsive, 2D plasmonic nanostructures with switchable chromogenic properties upon wetting-dewetting transitions is reported. By exploiting DNA hybridization-directed anchoring of gold nanoparticles (AuNPs) on substrates, a series of single-nanoparticle-layer (SNL) plasmonic films is fabricated. Due to the collective plasmonic responses in SNL, these ultrathin 2D films display rapid and reversible red-blue color change upon the wetting-dewetting transition, suggesting that hydration-induced microscopic plasmonic coupling between AuNPs is replicated in the macroscopic, centimeter-scale films...
July 17, 2017: Advanced Materials
Peter Bánovčin, Ingrid Škorňová, Matej Samoš, Martin Schnierer, Tomáš Bolek, František Kovář, Ján Staško, Peter Kubisz, Marián Mokáň
BACKGROUND: Activated factor X (factor Xa) plays an important role in regulation of platelets. The aim of this study was to test the effect of direct oral factor Xa inhibitors - rivaroxaban and apixaban - on platelet aggregation in patients with non-valvular atrial fibrillation (NV-AF). PATIENTS AND METHODS: This single-centre pilot study enrolled 21 factor Xa inhibitors-treated (9 rivaroxaban-treated and 12 apixaban-treated) patients with NV-AF. The trough and peak samples of these patients were tested for adenosinediphosphate-induced (ADP), epinephrine-induced, and collagen-induced platelet aggregation with light transmission aggregometry (LTA), and with factor Xa-calibrated anti-Xa chromogenic analysis...
July 13, 2017: Journal of Cardiovascular Pharmacology
Priyam Batra, Vishant Deo, Purva Mathur, Amit Kumar Gupta
Antimicrobial resistance is one of the greatest threats to human health worldwide. The rate of development of newer antibiotics is much slower than the rate of development of antibiotic resistance. A survey reported that it takes 15 years and US$800 million (including preclinical and clinical costs) to bring a single drug to the market, whereas the reuse of the older drugs for antimicrobial use takes $17 million, thereby circumventing 40% of the overall cost. The first case is a patient with nosocomial pyrexia of unknown origin who was given treatment with tigecycline and cefepime/tazobactam but failed to respond to the same...
July 2017: Journal of Laboratory Physicians
Anusha Chaturvedi, Gunjiganur Shankarappa Banashankari
INTRODUCTION: With the emergence of multidrug resistant Gram-negative bugs, there is an urgent need for rapid detection of these resistant organisms. AIM: This study was performed to assess the utility of a novel chromogenic medium in the detection of carbapenemase producing Enterobacteriaceae from various clinical specimens. MATERIALS AND METHODS: A total of 202 isolates of Enterobacteriaceae, which were resistant to meropenem (10 μg) disc by standard disc diffusion method, were analyzed over a period of 6 months...
July 2017: Journal of Laboratory Physicians
Xiaoqiang Wang, Chao Wang, Meihong Pan, Junting Wei, Fuping Jiang, Rongsheng Lu, Xuan Liu, Yihui Huang, Fang Huang
Taking inspiration from biology's effectiveness in functionalizing protein-based nanocages for chemical processes, we describe here a rational design of an artificial metalloenzyme for oxidations with the bacterial chaperonin GroEL, a nanocage for protein folding in nature, by supramolecular anchoring of catalytically active hemin in its hydrophobic central cavity. The promiscuity of the chaperonin cavity is an essential element of this design, which can mimic the hydrophobic binding pocket in natural metalloenzymes to accept cofactor and substrate without requiring specific ligand-protein interactions...
July 13, 2017: ACS Applied Materials & Interfaces
Hao-Hua Deng, Xiu-Ling Lin, Yin-Huan Liu, Ke-Lin Li, Qiong-Qiong Zhuang, Hua-Ping Peng, Ai-Lin Liu, Xing-Hua Xia, Wei Chen
Capping molecules on the surface of nanomaterials not only enhance the dispersion and stability of nanomaterials but also greatly facilitate their surface modification and biological applications. However, most capping molecules can severely block the active sites of the catalytic core, thereby decreasing the enzymatic activity of nanomaterial-based enzyme mimics. This work demonstrates the superiority of chitosan (Ch) as a capping molecule for synthesizing catalytic platinum nanoparticles (PtNPs). The experimental results show that Ch simultaneously exhibits an excellent stabilizing effect and enhances the oxidase-like activity of PtNPs...
July 12, 2017: Nanoscale
Patricia J Simner, Belita N A Opene, Krizia K Chambers, Matthew E Naumann, Karen C Carroll, Pranita D Tamma
Background: Accurate detection of carbapenemase-producing glucose nonfermenting gram-negative bacilli (CPNF), including Pseudomonas aeruginosa and Acinetobacter baumannii, is necessary to prevent their dissemination within health care settings. We performed a methods comparison study of 11 phenotypic carbapenemase-detection assays to evaluate their accuracy for the detection of CPNFs.Methods: 96 carbapenem-resistant glucose nonfermenting isolates were included, of which 29% produced carbapenemases. All CPNFs were molecularly characterized to identify β-lactamase genes...
July 12, 2017: Journal of Clinical Microbiology
Maddalena Maria Bolognesi, Marco Manzoni, Carla Rossana Scalia, Stefano Zannella, Francesca Maria Bosisio, Mario Faretta, Giorgio Cattoretti
Multiplexing, labeling for multiple immunostains in the very same cell or tissue section in situ, has raised considerable interest. The methods proposed include the use of labeled primary antibodies, spectral separation of fluorochromes, bleaching of the fluorophores or chromogens, blocking of previous antibody layers, all in various combinations. The major obstacles to the diffusion of this technique are high costs in custom antibodies and instruments, low throughput, and scarcity of specialized skills or facilities...
July 1, 2017: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
Zhiming Liu, Wenjian Wu
Hybrid materials of metal nanoparticles and biopolymers with catalytic properties are very promising to be used as detectors in biochemical reactions. In this work, the catalytic properties and relevant <i>in-situ</i> self-assembly abilities of hybrid films of gold nanoparticles (GNPs) and cellulose for the oxidation of benign chromogen 3,3',5,5'-tetramethylbenzidine (TMB) with hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) are revealed for the first time. The peroxidase-like properties of hybrid films are inherited from those of colloidal GNPs and increase with their contents of GNPs...
July 10, 2017: Nanotechnology
Shuqin Wang, Wenfang Deng, Lu Yang, Yueming Tan, Qingji Xie, Shouzhuo Yao
Cu-MOF nanoparticles with an average diameter of 550 nm were synthesized from 2-aminoterephthalic acid and Cu(NO3)2 by a mixed solvothermal method. The Cu-MOF nanoparticles can show peroxidase-like activity that can catalyze 3,3',5,5'-tetramethylbenzidine to produce a yellow chromogenic reaction in the presence of H2O2. The presence of abundant amine groups on the surfaces of Cu-MOF nanoparticles enables facile modification of Staphylococcus aureus (S. aureus) aptamer on Cu-MOF nanoparticles. By combining Cu-MOF-catalyzed chromogenic reaction with aptamer recognition and magnetic separation, a simple, sensitive, and selective colorimetric method for the detection of S...
July 10, 2017: ACS Applied Materials & Interfaces
A H Al-Bagawi, W Ahmad, Z M Saigl, H Alwael, E A Al-Harbi, M S El-Shahawi
The most common problems in spectrophotometric determination of various complex species originate from the background spectral interference. Thus, the present study aimed to overcome the spectral matrix interference for the precise analysis and speciation of mercury(II) in water by dual-wavelength β-correction spectrophotometry using 4-(2-thiazolylazo) resorcinol (TAR) as chromogenic reagent. The principle was based on measuring the correct absorbance for the formed complex of mercury(II) ions with TAR reagent at 547nm (lambda max)...
July 1, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Yothin Teethaisong, Katie Evans, Ismini Nakouti, Kanokwan Tiamyom, James R Ketudat-Cairns, Glyn Hobbs, Griangsak Eumkeb
A resazurin chromogenic agar (RCA) along with combined disc method has been developed as a promising method for rapid screening of extended-spectrum-β-lactamase (ESBL), AmpC β-lactamase, and co-production of ESBL and AmpC. Cefpodoxime (CPD) discs supplemented with and without clavulanic acid (CA), cloxacillin (CX), or CA+CX were evaluated against 86-molecularly confirmed β-lactamase-producing Enterobacteriaceae, including 15 ESBLs, 32 AmpCs, 9 co-producers of ESBL and AmpC, and 30 carbapenemase producers...
July 7, 2017: Microbiology and Immunology
Syed Samim Ali, Ankita Gangopadhyay, Kalipada Maiti, Sanchita Mondal, Ajoy Kumar Pramanik, Uday Narayan Guria, Md Raihan Uddin, Sukhendu Mandal, Debasish Mandal, Ajit Kumar Mahapatra
A new cyanine dye (CYD) based on hybrid hydroxynaphthalene-hemicyanine has been synthesized and characterized. The chromogenic and ratiometric fluorogenic probe (CYD) enables a fast and highly sensitive response to an OP nerve agent mimic diethyl chlorophosphate (DCP) through tandem phosphorylation and intramolecular cyclization reaction within 1 min and with the detection limit as low as 18.86 nM. To our knowledge this is the first report of a hydroxyl assisted bathochromic shift in a selective chemodosimeter for DCP exhibiting a ratiometric response...
July 19, 2017: Organic & Biomolecular Chemistry
Tatsuya Saito, Jyunichi Mukae, Yosuke Nakamura, Hiroshi Inaba, Keiji Nogami, Takatoshi Koyama, Katsuyuki Fukutake, Koh Yamamoto
A 53-year-old man, who had been diagnosed with mild hemophilia A (HA) at 35 years of age, was hospitalized with a thigh hematoma. His bleeding continued despite the administration of recombinant factor VIII (FVIII). The results of an FVIII/von Willebrand factor binding assay were normal. The patient's FVIII coagulant activity (FVIII:C) was low, but his FVIII antigen levels were within the normal limits, suggesting FVIII protein dysfunction. The FVIII:C measurements obtained by one-stage clotting and chromogenic assays were different...
2017: Internal Medicine
Juan Jose Mercado, James M Markert, William Meador, Philip Chapman, Arie Perry, James R Hackney
The true incidence of light chain deposition disease (LCDD) restricted to the central nervous system (CNS) is unknown. To our knowledge only 7 cases of LCDD restricted to the brain have been previously reported. We herein describe an unusual example. A 44-year-old man presented with a history of ischemic retinopathy in 2004 and left lower extremity hypoesthesia in 2007 that progressed gradually to left-sided weakness and numbness in the 2 years prior to his hospitalization in 2015. A stereotactic brain biopsy was performed, displaying nonspecific hyaline deposits of amorphous "amyloid-like" material involving deep brain white matter and vessels...
June 1, 2017: International Journal of Surgical Pathology
Domonique A Carson, Herman W Barkema, Sohail Naushad, Jeroen De Buck
Non-aureus staphylococci (NAS), the bacteria most commonly isolated from the bovine udder, potentially protect the udder against infection by major mastitis pathogens due to bacteriocin production. In this study, we determined the inhibitory capability of 441 bovine NAS isolates (comprising 26 species) against bovine S. aureus Furthermore, inhibiting isolates were tested against a human methicillin-resistant S. aureus (MRSA) isolate using a cross-streaking method. We determined the presence of bacteriocin clusters in NAS whole genomes using genome mining tools, BLAST, and comparison of genomes of closely related inhibiting and non-inhibiting isolates and determined the genetic organization of any identified bacteriocin biosynthetic gene clusters...
June 30, 2017: Applied and Environmental Microbiology
Alessandra Vecchione, Walter Florio, Francesco Celandroni, Simona Barnini, Antonella Lupetti, Emilia Ghelardi
AIMS: The present study was undertaken to evaluate the discrimination ability of six chromogenic media in presumptive yeast identification. METHODS: We analysed 108 clinical isolates and reference strains belonging to eight different species: Candida albicans,Candida dubliniensis, Candida tropicalis, Candida krusei, Candida glabrata, Candida parapsilosis,Candida lusitaniae and Trichosporon mucoides. RESULTS: C. albicans, C. tropicalis and C...
June 29, 2017: Journal of Clinical Pathology
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