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Alfred Weber, Andrea Engelmaier, Gabriele Mohr, Sonja Haindl, Hans Peter Schwarz, Peter L Turecek
BAX 855 (ADYNOVATE) is a PEGylated recombinant factor VIII (rFVIII) that showed prolonged circulatory half-life compared to unmodified rFVIII in hemophilic patients. Here, the development and validation of a novel assay is described that selectively measures the activity of BAX 855 as cofactor for the serine protease factor IX, which actives factor X. This method type, termed modification-dependent activity assay, is based on PEG-specific capture of BAX 855 by an anti-PEG IgG preparation, followed by a chromogenic FVIII activity assay...
September 29, 2016: Journal of Pharmaceutical and Biomedical Analysis
Cristina Puy, Erik I Tucker, Ivan S Ivanov, David Gailani, Stephanie A Smith, James H Morrissey, András Gruber, Owen J T McCarty
INTRODUCTION: Factor (F) XI supports both normal human hemostasis and pathological thrombosis. Activated FXI (FXIa) promotes thrombin generation by enzymatic activation of FXI, FIX, FX, and FV, and inactivation of alpha tissue factor pathway inhibitor (TFPIα), in vitro. Some of these reactions are now known to be enhanced by short-chain polyphosphates (SCP) derived from activated platelets. These SCPs act as a cofactor for the activation of FXI and FV by thrombin and FXIa, respectively...
2016: PloS One
C O'Connor, M G Kiernan, C Finnegan, J Powell, L Power, N H O'Connell, C P Dunne
BACKGROUND: The Mid-West of Ireland has higher than average national rates of invasive extended-spectrum beta-lactamase (ESBL) bloodstream infections and carbapenemase-producing Enterobacteriaceae (CPE), with increasing numbers of ESBL isolates detected in community-dwelling patients. AIMS: To conduct a point prevalence study in a convenience sample of the Mid-West population with the aim of determining the extent of ESBL colonisation. METHODS: Utilising anonymised community stool samples that had completed routine analysis, we conducted a point prevalence study over a 4-week period on all samples that met defined inclusion and exclusion criteria...
September 24, 2016: Irish Journal of Medical Science
I Klimiene, M Virgailis, A Pavilonis, R Siugzdiniene, R Mockeliunas, M Ruzauskas
The objectives of this study were to determine the prevalence and antimicrobial resistance of coagulase-negative staphylococci (CNS) isolated from dairy cows with subclinical mastitis. Antimicrobial resistance in staphylococci were evaluated by breakpoint values specific to the species (EU-CAST). The presence of resistance-encoding genes was detected by multiplex PCR. A total of 191 CNS isolates were obtained. The CNS isolates were typically resistant to penicillin (67.4%), tetracyc-line (18.9%), and erythromycin (13...
September 1, 2016: Polish Journal of Veterinary Sciences
Veikko Uahengo, Yan Zhang, Bi Xiong, Pingping Zhao, Ping Cai, Lydia Rhyman, Ponnadurai Ramasami, Kai Hu, Gongzhen Cheng
The 2,2'-dinaphtholazobenzene molecular framework (P) was designed, synthesized and characterized. Its absorption and fluorescence properties revealed that P is a dual sensor for copper ions (Cu(2+)) and fluoride ions (F(-)) in DMSO. The colorimetric activities were clearly visible by naked eye upon the addition of the two ions. Fluorescence quenching and enhancement were observed when Cu(2+) and F(-) ions were added respectively. Density Functional Theory (DFT) calculations were carried out to provide an insight into the interaction of guest ions (Cu(2+) and F(-)) with P, and to explain how the molecular orbitals were affected...
October 11, 2016: Journal of Fluorescence
Marcin Drozd, Mariusz Pietrzak, Paweł G Parzuchowski, Elżbieta Malinowska
Catalytic nanomaterials, widely used as substitutes of peroxidase, exhibit unique properties, which are unattainable for native enzymes. However, their activity is usually examined by means of substrates developed and methods standardized for horseradish peroxidase (HRP). The aim of the presented work was to determine the scope of usefulness of chromogenic substrates for gold nanoparticle (AuNP) activity studies under conditions which significantly extend beyond the activity range of a native HRP. The applicability of chromogens such as 3,3'5,5'-tetramethylbenzidine (TMB), o-phenylenediamine (OPD), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) beyond the typical range of pH, and for the samples of high concentration of hydrogen peroxide was examined...
October 8, 2016: Analytical and Bioanalytical Chemistry
Andrea Monas, Krunoslav Užarević, Ivan Halasz, Marina Juribašić Kulcsár, Manda Ćurić
Room-temperature accelerated aging in the solid state has been applied for atom- and energy-efficient activation of either one or two C-H bonds of azobenzene and methyl orange by palladium(ii) acetate. Organopalladium complexes are prepared in quantitative reactions without potentially harmful side products. Dicyclopalladated methyl orange is water-soluble and is a selective chromogenic biothiol sensor at physiologically-relevant micromolar concentrations in buffered aqueous media.
September 9, 2016: Chemical Communications: Chem Comm
Camila Peres Rubio, Josefa Hernández-Ruiz, Silvia Martinez-Subiela, Asta Tvarijonaviciute, Marino Bañón Arnao, José Joaquín Ceron
We performed analytical validation of 3 automated assays of the total antioxidant capacity (TAC) in canine serum and evaluated their use in dogs with inflammatory bowel disease. The assays were based on the generation of a 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) radical cation (ABTS•(+)) in aqueous media, which produces a blue-green color. The antioxidants present in the sample remove the chromogen in proportion to their concentrations. The assays differed mainly in the way in which this radical was produced...
November 2016: Journal of Veterinary Diagnostic Investigation
David J Seiffge, Christopher Traenka, Alexandros Polymeris, Lisa Hert, Urs Fisch, Nils Peters, Gian Marco De Marchis, Raphael Guzman, Christian H Nickel, Philipp A Lyrer, Leo H Bonati, Dimitrios Tsakiris, Stefan Engelter
Plasma levels of Rivaroxaban (RivLev) might be useful to guide therapeutic decisions in patients with acute stroke under Rivaroxaban. A prerequisite for the potential clinical usefulness is their rapid availability in emergency situations. Single-center explorative analysis from the Novel-Oral-Anticoagulants-in-Stroke-Patients-registry (NOACISP, We included consecutive patients with acute ischemic or hemorrhagic stroke under Rivaroxaban (last intake <48 h) in which RivLev determined by an automated anti-factor Xa-based chromogenic assay (Hyphen-Biomed, France) are available...
October 1, 2016: Journal of Thrombosis and Thrombolysis
Shunsuke Tawara, Takumi Sakai, Osamu Matsuzaki
BACKGROUND: Thrombomodulin (TM) alfa, a recombinant human soluble TM, enhances activation of pro-carboxypeptidase B2 (pro-CPB2) by thrombin. Activated pro-CPB2 (CPB2) exerts anti-inflammatory and anti-fibrinolytic activities. Therefore, TM alfa may also have anti-inflammatory and anti-fibrinolytic effects through CPB2. However, these effects of TM alfa have not been elucidated. In the present study, we investigated the effects of TM alfa on inactivation of complement component C5a as an anti-inflammatory effect and prolongation of clot lysis time as an anti-fibrinolytic effect via CPB2 in vitro...
September 17, 2016: Thrombosis Research
Ajeet Kumar Lakhera, Vineet Kumar
Acacia tortilis ssp. raddiana (Savi) Brenan commonly known as Israeli Babool has contributed immensely for sand dunes management in Indian desert leading to wind erosion control and increased biological productivity. The species is extensively used in traditional medicine system for a number of therapeutic applications and as nutraceutical. The polysaccharide was isolated in 43.6% yield from gum exudates. The monosaccharides, L-arabinose, D-galactose D-glucose, L-rhamnose and D-mannose were determined in molar ratio of 78...
September 29, 2016: International Journal of Biological Macromolecules
M Bochniarz, Ł Adaszek, B Dzięgiel, A Nowaczek, W Wawron, R Dąbrowski, M Szczubiał, S Winiarczyk
The aim of this study was to recognize selected factors of virulence determining the adhesion of Staphylococcus chromogenes to cows' udder tissues in subclinical mastitis and to evaluate the susceptibility of this pathogen to antibiotics. The subjects of the study were 38 isolates of Staph. chromogenes from 335 samples of milk from cows with subclinical coagulase-negative staphylococci mastitis. Somatic cell count ranged between 216,000 and 568,000/mL of milk (average 356,000/mL of milk). We confirmed the ability to produce slime in 24 isolates (63...
September 28, 2016: Journal of Dairy Science
Pascale Trepanier, Kim Mallard, Danièle Meunier, Rachel Pike, Derek Brown, Janet P Ashby, Hugo Donaldson, F Mustafa Awad-El-Kariem, Indran Balakrishnan, Marc Cubbon, Paul R Chadwick, Michael Doughton, Rachael Doughton, Fiona Hardiman, Graham Harvey, Carolyne Horner, John Lee, Jonathan Lewis, Anne Loughrey, Rohini Manuel, Helena Parsons, John D Perry, Gemma Vanstone, Graham White, Nandini Shetty, John Coia, Camilla Wiuff, Katie L Hopkins, Neil Woodford
OBJECTIVES: To estimate UK prevalence and incidence of clinically significant carbapenemase-producing Enterobacteriaceae (CPE), and to determine epidemiological characteristics, laboratory methods and infection prevention and control (IPC) measures in acute care facilities. METHODS: A 6 month survey was undertaken in November 2013-April 2014 in 21 sentinel UK laboratories as part of the European Survey on Carbapenemase-Producing Enterobacteriaceae (EuSCAPE) project...
September 29, 2016: Journal of Antimicrobial Chemotherapy
Julia Schückel, Stjepan Krešimir Kračun, William G T Willats
Carbohydrates active enzymes (CAZymes) have multiple roles in vivo and are widely used for industrial processing in the biofuel, textile, detergent, paper and food industries. A deeper understanding of CAZymes is important from both fundamental biology and industrial standpoints. Vast numbers of CAZymes exist in nature (especially in microorganisms) and hundreds of thousands have been cataloged and described in the carbohydrate active enzyme database (CAZy). However, the rate of discovery of putative enzymes has outstripped our ability to biochemically characterize their activities...
2016: Journal of Visualized Experiments: JoVE
Jin Gao, Laura Couzens, Maryna C Eichelberger
Antibodies to neuraminidase (NA), the second most abundant surface protein on influenza virus, contribute toward protection against influenza. Traditional methods to measure NA inhibiting (NI) antibody titers are not practical for routine serology. This protocol describes the enzyme-linked lectin assay (ELLA), a practical alternative method to measure NI titers that is performed in 96 well plates coated with a large glycoprotein substrate, fetuin. NA cleaves terminal sialic acids from fetuin, exposing the penultimate sugar, galactose...
2016: Journal of Visualized Experiments: JoVE
Fabrice Compain, Salah Gallahd, Catherine Eckert, Guillaume Arlet, Astrid Ramahefasolo, Dominique Decré, Marie Lavollay, Isabelle Podglajen
Carbapenemase-producing Enterobacteriaceae have become a major therapeutic challenge in hospital- and community-acquired infections worldwide (1).….
September 28, 2016: Journal of Clinical Microbiology
Abdoul-Salam Ouedraogo, Catherine Dunyach-Remy, Aimée Kissou, Soufiane Sanou, Armel Poda, Carole G Kyelem, Jérôme Solassol, Anne-Laure Bañuls, Philippe Van De Perre, Rasmata Ouédraogo, Hélène Jean-Pierre, Jean-Philippe Lavigne, Sylvain Godreuil
The objectives of the present study were to investigate the rate of S.aureus nasal carriage and molecular characteristics in hospital and community settings in Bobo Dioulasso, Burkina Faso. Nasal samples (n = 219) were collected from 116 healthy volunteers and 103 hospitalized patients in July and August 2014. Samples were first screened using CHROMagar Staph aureus chromogenic agar plates, and S. aureus strains were identified by mass spectrometry. Antibiotic susceptibility was tested using the disk diffusion method on Müller-Hinton agar...
2016: Frontiers in Microbiology
Meili Zhao, Xiufen Zhou, Jian Tang, Zhifu Deng, Xuan Xu, Zhi Chen, Xutian Li, Liting Yang, Li-Jun Ma
Pyrene excimer usually serves as a chromogenic unit for developing ratiometric fluorescent sensors. But this study used excimer as a large hydrophobic group to regulate the molecular hydrophobicity, and obtained a new fluorescent sensor, N, N-bi[4(1-pyrene)-butyroyl]ornithine (1), for detection and removal of Fe(3+) and Pb(2+) from aqueous solutions. The coordination of 1 and Fe(3+) in the aqueous solution or even pure water forms removable flocculent precipitates, accompanied by obvious fluorescent quenching of emission spectra...
September 21, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Yan Gao, Yiting Wu, Junwei Di
We have coupled gold nanoparticles (AuNPs) with silver nanoparticles (AgNPs) to assemble a plasmonic sensing platform for colorimetric detection of glucose. In this system, small AuNPs (~4nm) can act as glucose oxidase (GOD) mimic enzyme to catalytically oxidize glucose in the presence of oxygen, producing hydrogen peroxide, which dissolves AgNPs to lead the color changes. Glucose can be detected not only by naked eyes (from yellow to red) but also by spectrophotometer in the concentration range of 5-70μM, with detection limit of 3μM...
September 20, 2016: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Thitiporn Teeravivattanakit, Sirilak Baramee, Paripok Phitsuwan, Rattiya Waeonukul, Patthra Pason, Chakrit Tachaapaikoon, Kazuo Sakka, Khanok Ratanakhanokchai
: The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a family 43 glycoside hydrolase and a family 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, and arabinoxylan arabinofuranohydrolase activities. PcAxy43A hydrolyzed xylohexaose and birch wood xylan to release a series of xylooligosaccharides, indicating that PcAxy43A contained endo-xylanase activity...
September 23, 2016: Applied and Environmental Microbiology
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