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https://www.readbyqxmd.com/read/28092466/clinical-performance-of-the-solana%C3%A2-point-of-care-trichomonas-assay-from-clinician-collected-vaginal-swabs-and-urine-specimens-from-symptomatic-and-asymptomatic-women
#1
C A Gaydos, J Schwebke, J Dombrowski, J Marrazzo, J Coleman, B Silver, M Barnes, L Crane, P Fine
BACKGROUND: Solana® (Quidel) is a new rapid (<40 min.) point-of-care (POC) test for qualitative detection of Trichomonas vaginalis (TV) DNA. The assay has two steps: 1) specimen preparation, and 2) amplification and detection using isothermal Helicase-Dependent Amplification (HDA). The objective was to demonstrate the performance of Solana for vaginal swabs and female urines based on comparison to wet mount and TV culture. Performance was also compared to the Aptima-TV assay. METHODS: Urine and four clinician-collected vaginal swabs were collected...
January 16, 2017: Expert Review of Molecular Diagnostics
https://www.readbyqxmd.com/read/28092165/recommendations-for-best-disinfectant-practices-to-reduce-the-spread-of-infection-via-wrestling-mats
#2
Linda Mull Young, Vicki Abram Motz, Emily R Markey, Suzanne C Young, Ronald E Beaschler
CONTEXT:  At the request of the National Wrestling Coaches Association and the head wrestling coach at our university, we conducted a study of infection transmission in collegiate wrestlers. OBJECTIVE:  To examine disinfectant effectiveness and develop best-practice guidelines for minimizing the spread of skin infections via wrestling mats. DESIGN:  Controlled laboratory study and crossover study. SETTING:  Laboratory and two 15-college wrestling invitational meets...
January 16, 2017: Journal of Athletic Training
https://www.readbyqxmd.com/read/28092107/rsv-associated-hospitalizations-in-children-in-karachi-pakistan-implications-for-vaccine-prevention-strategies
#3
Asad Ali, Mohammad Tahir Yousafzai, Rabbia Waris, Fatima Jafri, Fatima Aziz, Imran Naeem Abbasi, Anita Zaidi
Major progress is being made in vaccines against Respiratory Syncytial Virus (RSV), with multiple vaccine candidates currently in the clinical phase of development. Making an investment case for public sector financing of RSV vaccine will require estimation of burden, cost-effectiveness and impact. The aim of this study is to determine the proportion, age distribution and clinical spectrum of RSV associated hospitalizations in children in Karachi, Pakistan. A three years prospective study was conducted at the Aga Khan University hospital in Karachi, a city of 20 million in south Pakistan, from August 2009 to June 2012...
January 16, 2017: Journal of Medical Virology
https://www.readbyqxmd.com/read/28091839/impact-of-the-2014-2015-influenza-season-on-the-activity-of-an-academic-emergency-department
#4
Nicolas Beysard, Bertrand Yersin, Pascal Meylan, Olivier Hugli, Pierre-Nicolas Carron
The morbidity and mortality of the 2014-2015 influenza season were more important than those in previous years. We assessed the impact of the 2014-2015 influenza season on the length of stay (LOS) and workload in an academic emergency department (ED). This is a monocentric retrospective study. The database of the microbiology laboratory was used to identify influenza nasal swabs performed during the influenza seasons from 2010 to 2015. Patients admitted to the ED during these periods were identified through the administrative database and cross-checked with patients who underwent an influenza nasal swab in the ED...
January 16, 2017: Internal and Emergency Medicine
https://www.readbyqxmd.com/read/28088665/influenza-a-h1n1-pdm-2009-and-influenza-b-virus-co-infection-in-hospitalized-and-non-hospitalized-patients-during-the-2015-2016-epidemic-season-in-israel
#5
Rakefet Pando, Yaron Drori, Nehemya Friedman, Aharona Glatman-Freedman, Hanna Sefty, Tamar Shohat, Ella Mendelson, Musa Hindiyeh, Michal Mandelboim
BACKGROUND: Influenza A and B viruses co-infections are rare events and mainly occurred in immunocompromised patients. OBJECTIVES: In this study we report an unusually high occurrence of influenza A (H1N1)pdm 2009 and influenza B virus co-infections during the epidemic year 2015-2016. STUDY DESIGN: Nasopharyngeal swabs were collected from 1919 patients visiting 26 outpatient clinics distributed throughout Israel and presenting with influenza-like illness...
January 6, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28088212/ultrasensitive-detection-of-oncogenic-human-papillomavirus-in-oropharyngeal-tissue-swabs
#6
Andre Isaac, Morris Kostiuk, Han Zhang, Cameron Lindsay, Fawaz Makki, Daniel A O'Connell, Jeffrey R Harris, David W J Cote, Hadi Seikaly, Vincent L Biron
BACKGROUND: The incidence of oropharyngeal squamous cell carcinoma (OPSCC) caused by oncogenic human papillomavirus (HPV) is rising worldwide. HPV-OPSCC is commonly diagnosed by RT-qPCR of HPV E6 and E7 oncoproteins or by p16 immunohistochemistry (IHC). Droplet digital PCR (ddPCR) has been recently reported as an ultra-sensitive and highly precise method of nucleic acid quantification for biomarker analysis. To validate the use of a minimally invasive assay for detection of oncogenic HPV based on oropharyngeal swabs using ddPCR...
January 14, 2017: Journal of Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28087494/porcine-kobuvirus-1-in-healthy-and-diarrheic-pigs-genetic-detection-and-characterization-of-virus-and-co-infection-with-rotavirus-a
#7
Anna Jackova, Ivan Sliz, Rene Mandelik, Slavomira Salamunova, Jaroslav Novotny, Mariana Kolesarova, Michaela Vlasakova, Stefan Vilcek
The porcine kobuvirus 1 (PKV-1) is believed to be an enteric virus. To investigate the prevalence of PKV-1 in pigs, virus was detected by RT-PCR in rectal swabs originating from 414 healthy and diarrheic pigs of different age categories on farms in Slovakia. Among all ages of animals, PKV-1 was detected equally in diarrheic (63.8%) and clinically healthy (62.9%) pigs. PKV-1 was more often detected in diarrheic (74.6%) than in healthy (64.4%) suckling piglets (<28days) but data was not statistically significant...
January 10, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28086915/comparative-study-in-infertile-couples-with-and-without-chlamydia-trachomatis-genital-infection
#8
Yuanchang Zhu, Biao Yin, Tonghua Wu, Lijun Ye, Chunmei Chen, Yong Zeng, Yaou Zhang
BACKGROUND: Chlamydia, caused by the bacterium Chlamydia trachomatis(C. trachomatis), is the most common sexually transmitted disease. The incidence is not clear due to the asymptomatic nature of early stage of infections. The incidence of Chlamydia has not been fully investigated in the Chinese Han population. Since chronic infection with can C. trachomatis can lead to infertility in males and females, it is important to determine the impact of infection on clinical outcomes. The aim of this study is to explore the epidemiology of C...
January 13, 2017: Reproductive Health
https://www.readbyqxmd.com/read/28078492/first-report-of-bla-ctx-m-15-type-esbl-producing-klebsiella-pneumoniae-in-wild-migratory-birds-in-pakistan
#9
Shahbaz Raza, Mashkoor Mohsin, Waqas Ahmed Madni, Fatima Sarwar, Muhammad Saqib, Bilal Aslam
We investigated wild migratory birds faecal swabs for extended-spectrum β-lactamases-producing Klebsiella pneumoniae (ESBL-K. pneumoniae) from wetland habitats in Pakistan. ESBL-K. pneumoniae were analysed for MDR phenotype, ESBL genotype and genetic diversity. A total of 13 (8.6%) ESBL-K. pneumoniae were recovered. Of these, 8 (61%) isolates were MDR. DNA sequencing confirmed bla CTX-M-15 as the dominant ESBL genotype. BOX-PCR fingerprints showed most of the isolates are unrelated. This study is the first to report the wildlife contamination of CTX-M-15-producing K...
January 11, 2017: EcoHealth
https://www.readbyqxmd.com/read/28077700/evaluation-of-alere%C3%A2-i-rsv-for-rapid-detection-of-respiratory-syncytial-virus-in-children-hospitalized-with-acute-respiratory-tract-infection
#10
Rebecca Marie Peters, Sarah Valerie Schnee, Julia Tabatabai, Paul Schnitzler, Johannes Pfeil
Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized with acute respiratory tract infection during winter season 2015/2016. Alere™ i RSV assay test results were compared to Altona® RealStar RSV real-time reverse transcription PCR (RT-PCR)...
January 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077698/detection-of-antigenic-variants-of-swine-subtype-h3-influenza-a-viruses-from-clinical-samples
#11
Brigitte E Martin, Andrew S Bowman, Lei Li, Jacqueline M Nolting, David R Smith, Larry A Hanson, Xiu-Feng Wan
A large population of genetically and antigenically diverse influenza A viruses (IAVs) are circulating among the swine population, playing an important role in influenza ecology. Swine IAVs not only cause outbreaks among swine, but they can also be transmitted to humans, causing sporadic infections and even pandemic outbreaks. Antigenic characterization of swine IAVs are key to understanding the natural history of these viruses in swine and to selecting strains for effective vaccines. However, influenza outbreaks generally spread rapidly among swine, and the conventional methods for antigenic characterization require virus propagation, a time-consuming process that can significantly reduce the effectiveness of vaccination programs...
January 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077695/stability-studies-on-dry-swabs-and-wet-mailed-swabs-for-the-detection-of-c-trachomatis-and-n-gonorrhoeae-in-the-aptima-assays
#12
Jeanne Moncada, Carey B Clark, Jeffrey Holden, Edward W Hook, Charlotte A Gaydos, Julius Schachter
The Aptima Combo2 (AC2) and Aptima CT (ACT) (Hologic Inc., San Diego, CA) are nucleic acid amplified tests (NAATs) that detect Chlamydia trachomatis (CT). AC2 also detects Neisseria gonorrhoeae (NG). Storage and temperature conditions may impact the utility of NAATs in some settings and screening programs. We evaluated specimen stability for use beyond the Aptima package insert temperature and duration of storage specifications (between 2° to 30°C, 60 days) in two studies: 1) dry CT seeded swabs were used with ACT after storage at 4°C, 23°C or 36°C for up to 84 days and 2) swabs seeded with CT and NG then placed in transport media were tested with AC2, after mailing via the U...
January 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28077283/aetiology-of-febrile-pharyngitis-in-children-potential-of-myxovirus-resistance-protein-a-mxa-as-a-biomarker-of-viral-infection
#13
Lauri Ivaska, Jussi Niemelä, Johanna Lempainen, Riikka Österback, Matti Waris, Tytti Vuorinen, Jukka Hytönen, Kaisu Rantakokko-Jalava, Ville Peltola
OBJECTIVES: Besides group A streptococcus (GAS), microbial causes of pharyngitis in children are not well known. We aimed to document the viral and bacterial aetiology of pharyngitis and to assess the pathogenic role of viruses by determining the myxovirus resistance protein A (MxA) in the blood as a marker of interferon response. METHODS: In this prospective observational study, throat swabs and blood samples were collected from children (age 1-16 years) presenting to the emergency department with febrile pharyngitis...
January 7, 2017: Journal of Infection
https://www.readbyqxmd.com/read/28077093/evaluation-of-the-point-of-care-becton-dickinson-veritor%C3%A2-rapid-influenza-diagnostic-test-in-kenya-2013-2014
#14
Linus K Ndegwa, Gideon Emukule, Timothy M Uyeki, Eunice Mailu, Sandra S Chaves, Marc-Alain Widdowson, Bandika V Lewa, Francis K Muiruri, Peter Omoth, Barry Fields, Joshua A Mott
BACKGROUND: We evaluated the performance of the Becton Dickinson Veritor™ System Flu A + B rapid influenza diagnostic test (RIDT) to detect influenza viruses in respiratory specimens from patients enrolled at five surveillance sites in Kenya, a tropical country where influenza seasonality is variable. METHODS: Nasal swab (NS) and nasopharyngeal (NP)/oropharyngeal (OP) swabs were collected from patients with influenza like illness and/or severe acute respiratory infection...
January 11, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28073985/population-based-pertussis-incidence-and-risk-factors-in-infants-less-than-6-months-in-nepal
#15
Michelle M Hughes, Janet A Englund, Jane Kuypers, James M Tielsch, Subarna K Khatry, Laxman Shrestha, Steven C LeClerq, Mark Steinhoff, Joanne Katz
BACKGROUND: Pertussis is estimated to cause 2 percent of childhood deaths globally and is a growing public health problem in developed countries despite high vaccination coverage. Infants are at greatest risk of morbidity and mortality. Maternal vaccination during pregnancy may be effective to prevent pertussis in young infants, but population-based estimates of disease burden in infants are lacking, particularly in low-income countries. The objective of this study was to estimate the incidence of pertussis in infants less than 6 months of age in Sarlahi District, Nepal...
January 10, 2017: Journal of the Pediatric Infectious Diseases Society
https://www.readbyqxmd.com/read/28073612/gonorrhea-and-chlamydia-in-the-emergency-department-continued-need-for-more-focused-treatment-for-men-women-and-pregnant-women
#16
Sean P Wilson, Taher Vohra, McKenna Knych, Jared Goldberg, Christopher Price, Sean Calo, Meredith Mahan, Joseph Miller
INTRODUCTION: Delay in current nucleic acid amplification testing for Neisseria gonorrhoeae and Chlamydia trachomatis has led to recommendations for presumptive treatment in patients with concern for infection and unreliable follow-up. In the urban setting, it is assumed that many patients have unreliable follow-up, therefore presumptive therapy is thought to be used frequently. We sought to measure the frequency of disease and accuracy of presumptive treatment for these infections. METHODS: This was an observational cohort study performed at an urban academic Level 1 trauma center ED with an annual census of 95,000 visits per year...
January 3, 2017: American Journal of Emergency Medicine
https://www.readbyqxmd.com/read/28072611/baby-shampoo-versus-povidone-iodine-or-isopropyl-alcohol-in-reducing-eyelid-skin-bacterial-load
#17
Giancarlo A Garcia, Christine V Nguyen, Marc A Yonkers, Jeremiah P Tao
PURPOSE: Baby shampoo is used as an alternative surgical skin preparation, but the evidence supporting its use is scarce with no descriptions of efficacy in the periocular region. The authors compare the efficacy of baby shampoo, povidone-iodine (PI, Betadine) and isopropyl alcohol (IA) in reducing eyelid skin bacterial load. METHODS: Prospective, randomized, comparative, and interventional trial. Bacterial load on adult, human eyelid skin was quantitated before and after cleansing with 1) dilute baby shampoo, 2) 10% PI, or 3) 70% IA...
January 6, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28072501/nasopharyngeal-carriage-of-respiratory-pathogens-in-warao-amerindians-significant-relationship-with-stunting
#18
Lilly M Verhagen, Meyke Hermsen, Ismar A Rivera-Olivero, María Carolina Sisco, Marien I de Jonge, Peter W M Hermans, Jacobus H de Waard
OBJECTIVE: To assess risk factors for nasopharyngeal carriage of pathogens in geographically isolated Warao Amerindians in Venezuela. METHODS: In this point prevalence survey, nasopharyngeal swabs were obtained from 1064 Warao Amerindians: 504 children aged 0 - 4 years, 227 children aged 5 - 10 years, and 333 caregivers. Written questionnaires were completed to obtain information on demographics and environmental risk factors. Anthropometric measurements were performed in children aged 0 - 4 years...
January 10, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28072493/patterns-of-infections-aetiological-agents-and-antimicrobial-resistance-at-a-tertiary-care-hospital-in-northern-tanzania
#19
Happiness Houka Kumburu, Tolbert Sonda, Blandina Theophil Mmbaga, Michael Alifrangis, Ole Lund, Gibson Kibiki, Frank M Aarestrup
OBJECTIVE: To determine the causative agents of infections and their antimicrobial susceptibility at a tertiary care hospital in Moshi, Tanzania, to guide optimal treatment. METHODS: A total of 590 specimens (stool (56), sputum (122), blood (126) and wound swabs (286)) were collected from 575 patients admitted in the medical and surgical departments. The bacterial species were determined by conventional methods and disk diffusion was used to determine the antimicrobial susceptibility pattern of the bacteria isolates...
January 10, 2017: Tropical Medicine & International Health: TM & IH
https://www.readbyqxmd.com/read/28071687/bacterial-load-of-conditioned-pressure-ulcers-is-not-a-predictor-for-early-flap-failure-in-spinal-cord-injury
#20
T Kamradt, S Klein, S Zimmermann, J Schröder-Braunstein, C H Fürstenberg, C Hensel, N Weidner, A Hug
OBJECTIVES: Pressure ulcers impose a major lifetime medical problem to patients with high-grade spinal cord injury (SCI). For patients with stages 3-4 pressure ulcers, plastic surgery is often the only remaining treatment option. Despite considerable flap failure rates of around 30%, only sparse knowledge exists on predictors for flap failure. Hence, identification of predictors for flap failures is needed. METHODS: We prospectively enrolled 38 SCI patients with stages 3-4 pressure ulcers scheduled for plastic surgery...
January 10, 2017: Spinal Cord
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