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https://www.readbyqxmd.com/read/28445636/disposable-autonomous-device-for-rapid-swab-to-result-diagnosis-of-influenza
#1
Shichu Huang, Koji Abe, Steven Bennett, Tinny Liang, Paula D Ladd, Lindsay Yokobe, Caitlin E Anderson, Kamal Shah, Josh Bishop, Mike Purfield, Peter C Kauffman, Sai Paul, AnneMarie E Welch, Bonnie Strelitz, Kristin Follmer, Kelsey Pullar, Luis Sanchez-Erebia, Emily Gerth-Guyette, Gonzalo J Domingo, Eileen Klein, Janet A Englund, Elain Fu, Paul Yager
A prototype of a self-contained, automated, disposable device for chemically-amplified protein-based detection of influenza virus from nasal swab specimens was developed and evaluated in a clinical setting. The device required only simple specimen manipulation without any dedicated instrumentation or specialized training by the operator for interpretation. The device was based on a sandwich immunoassay for influenza virus nucleoprotein; it used an enzyme-labeled antibody and a chromogenic substrate to provide an amplified visible signal, in a two-dimensional paper network format...
April 26, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28445011/rapid-detection-of-staphylococcus-aureus-and-methicillin-resistant-s-aureus-in-atopic-dermatitis-by-using-the-bd-max-staphsr-assay
#2
Mi Kyung Lee, Kui Young Park, Taewon Jin, Ju Hee Kim, Seong Jun Seo
Eczematous lesions of atopic dermatitis (AD) patients are known to be a source of Staphylococcus aureus (SA) transmission and might be a reservoir for community-associated methicillin-resistant SA (MRSA). The BD Max StaphSR (BD-SR) is a fully automated, multiplex real-time PCR assay for the direct detection and differentiation of SA and MRSA from nasal swab samples. We evaluated the detection rates of SA and MRSA from skin lesions of outpatients with AD using the BD-SR assay, and determined the usefulness of the BD-SR assay...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28445008/prevalence-of-complement-mediated-cell-lysis-like-gene-sicg-in-streptococcus-dysgalactiae-subsp-equisimilis-isolates-from-japan-2014-2016
#3
Takashi Takahashi, Tomohiro Fujita, Akiyoshi Shibayama, Yuzo Tsuyuki, Haruno Yoshida
BACKGROUND: Streptococcus dysgalactiae subsp. equisimilis (SDSE; a β-hemolytic streptococcus of human or animal origin) infections are emerging worldwide. We evaluated the clonal distribution of complement-mediated cell lysis-like gene (sicG) among SDSE isolates from three central prefectures of Japan. METHODS: Group G/C β-hemolytic streptococci were collected from three institutions from April 2014 to March 2016. Fifty-five strains (52 from humans and three from animals) were identified as SDSE on the basis of 16S rRNA sequencing data...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28444974/school-based-surveillance-for-influenza-vaccine-effectiveness-during-2014-15-seasons-in-hong-kong
#4
Ting F Leung, Renee W Y Chan, Angela Kwok, Wendy C S Ho, Mars K P Tao, Kam L Hon, Frankie W T Cheng, Albert M Li, Paul K S Chan
BACKGROUND: Influenza imposes substantial healthcare burden in children, which can be prevented by vaccination. Influenza vaccination coverage varies widely among childhood populations worldwide, which has significant impact on herd immunity and usefulness of influenza vaccine. However, there is limited real-life data on influenza vaccine effectiveness (VE) in children. OBJECTIVE: This prospective study aimed to investigate clinical spectrum of childhood influenza and VE in preventing influenza in Hong Kong children...
April 26, 2017: Influenza and Other Respiratory Viruses
https://www.readbyqxmd.com/read/28444876/cataracts-in-a-population-of-bengal-cats-in-france
#5
Aurélie Bourguet, Gilles Chaudieu, Alice Briatta, Alexandre Guyonnet, Marie Abitbol, Sabine Chahory
OBJECTIVE: To document the clinical appearance and prevalence of cataracts in a French population of Bengal cats. METHODS: Two distinct populations of Bengal cats were examined as follows: (i) 51 animals recruited for evaluation of national prevalence of ocular diseases in an observational study conducted between October 2014 and November 2016 at the Alfort ophthalmology unit; (ii) 12 patients referred for cataract diagnosis examined at a veterinary eye clinic located in central France, between December 2014 and February 2016...
April 25, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/28444619/the-reference-cube-a-new-ballistic-model-to-generate-staining-in-firearm-barrels
#6
Christian Schyma, Kristina Bauer, Julia Brünig
After contact shots to the head biological traces can be found inside firearm barrels. So far silicone coated, gelatin filled box models were used to generate such staining according to the triple contrast method (mixture of acrylic paint, barium sulfate and blood sealed in a thin foil bag). This study was conducted to develop a transparent ballistic model allowing contact shots. Gelatin filled polyethylene bottles with and without a silicone coat were tested in comparison to non-covered gelatin blocks. Finally, thin foil bags of 5 cm × 5 cm dimension were glued on a synthetic absorbent kitchen wipe on top of which 1 L 10% gelatin solution was molded to create blocks of 8...
April 25, 2017: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/28443807/-persistent-therapy-resistant-conjunctivitis-consider-infection-with-chlamydia-trachomatis
#7
L van Wagensveld, F A van der Sluijs, R H C Zegers
Conjunctivitis is a frequently diagnosed disease, usually caused by a virus. A less well-known cause is a chlamydia infection. This may result in missed diagnoses, delay of treatment and several complications. We present two cases of a persistent, therapy-resistant conjunctivitis in patients who were over 70 years of age. One patient had conjunctival follicles, characteristic of chlamydia conjunctivitis. The polymerase chain reaction tests of conjunctival samples from both patients were positive for chlamydia...
2017: Nederlands Tijdschrift Voor Geneeskunde
https://www.readbyqxmd.com/read/28443586/salmonella-enterica-serovar-enteritidis-liver-abscess-after-blunt-abdominal-trauma
#8
Agata Ladic, Igor Petrovic, Ante Gojevic, Emil Kinda, Ivo Coza
BACKGROUND: Salmonella enterica serovar Enteritidis is among the most reported serotypes of Salmonella species worldwide, but rarely reported as the causative agent of a liver abscess. CASE REPORT: We present a patient with an abdominal blunt trauma. An initial abdominal CT scan revealed rupture of the right kidney and of the liver. Two days later, his physical state deteriorated and a new CT scan was obtained. An eight-centimetre large liver abscess was verified...
April 13, 2017: Balkan Medical Journal
https://www.readbyqxmd.com/read/28442306/evaluation-of-a-real-time-pcr-assay-for-rectal-screening-of-oxa-48-producing-enterobacteriaceae-in-a-general-intensive-care-unit-of-an-endemic-hospital
#9
J Fernández, S A Cunningham, A Fernández-Verdugo, L Viña-Soria, L Martín, M R Rodicio, D Escudero, F Vazquez, J N Mandrekar, R Patel
Carbapenemase-producing Enterobacteriaceae are increasing worldwide. Rectal screening for these bacteria can inform the management of infected and colonized patients, especially those admitted to intensive care units (ICUs). A laboratory developed, qualitative duplex real-time polymerase chain reaction assay for rapid detection of OXA-48-like and VIM producing Enterobacteriaceae, performed on rectal swabs, was designed and evaluated in an intensive care unit with endemic presence of OXA-48. During analytical assay validation, no cross-reactivity was observed and 100% sensitivity and specificity were obtained for both blaOXA-48-like and blaVIM in all spiked clinical samples...
April 6, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28442206/change-the-habit-to-change-the-practice-do-audits-really-ever-change-anything
#10
D Bodansky, Y Oskrochi, G Judah, M Lewis, B Fischer, B Narayan
INTRODUCTION: Methicillin resistant staphylococcus aureus (MRSA) eradication by nurses and pre-op induction antibiotics are mandated for emergency surgery, where true status is unknown. We assessed adherence to local MRSA guidelines for patients undergoing emergency hip fracture surgery and employed point of decision prompts to influence clinicians' behaviour. METHODS: We undertook a retrospective record review for all patients undergoing emergency hip fracture surgery at a UK major trauma centre over 3-months...
April 17, 2017: Injury
https://www.readbyqxmd.com/read/28439608/isolation-of-mycoplasma-genitalium-from-endocervical-swabs-of-infertile-women
#11
Dunia A Alfarraj, Ali M Somily
To determine the occurrence of Mycoplasma genitalium (M. genitalium) infection in infertile women attending infertility clinic and its association to infertility. Methods: Endocervical specimens were collected from women presenting with primary and secondary infertility and from fertile women as control group. Real-time polymerase chain reaction (PCR)  assay was performed to detect the presence of M. genitalium from these endocervical specimens.  Results: Mycoplasma genitalium was detected in 3% of infertile women...
May 2017: Saudi Medical Journal
https://www.readbyqxmd.com/read/28439196/clinical-differences-of-influenza-subspecies-among-hospitalized-children
#12
Manolya Acar, Murat Sütçü, Hacer Aktürk, Selda Hançerli Törün, Metin Uysalol, Sevim Meşe, Nuran Salman, Ayper Somer
AIM: Clinical findings, mortality, and morbidity rates differ among influenza subspecies. Awareness of these differences will lead physicians to choose the proper diagnostic and therapeutic strategies and to foresee possible complications. The aim of this study was to evaluate the clinical differences of influenza subspecies among hospitalized children. MATERIAL AND METHODS: Hospitalized children with proven influenza infection by polymerase chain reaction on nasopharyngeal swab specimens in our clinic, between December 2013 and March 2016, were enrolled...
March 2017: Türk Pediatri Arşivi
https://www.readbyqxmd.com/read/28438677/a-prospective-comparative-study-of-severe-neurological-and-uncomplicated-hand-foot-and-mouth-forms-of-paediatric-enterovirus-71-infections
#13
Yoann Crabol, Polidy Pean, Channa Mey, Veasna Duong, Beat Richner, Denis Laurent, Ky Santy, Heng Sothy, Philippe Dussart, Arnaud Tarantola, Philippe Buchy, Paul F Horwood
OBJECTIVES: In this study, we document the clinical characteristics and investigated risk factors for uncomplicated and severe forms of EV-A71 disease in Cambodian children. METHODS: From March to July 2014 inclusive, all patients with suspicion of EV-A71 infection presenting to Kantha Bopha Hospitals in Phnom Penh and Siem Reap and confirmed by the Virology Unit at the Institut Pasteur du Cambodge were prospectively enrolled in this study. Throat swabs, rectal swabs and serum samples were collected from all consecutive patients with suspected EV-A71 infection...
April 21, 2017: International Journal of Infectious Diseases: IJID
https://www.readbyqxmd.com/read/28438522/detection-of-leishmania-infantum-dna-in-conjunctival-swabs-of-cats-by-quantitative-real-time-pcr
#14
Julia Cristina Benassi, Graziella U Benvenga, Helena Lage Ferreira, Vanessa F Pereira, Lara B Keid, Rodrigo Soares, Tricia Maria Ferreira de Sousa Oliveira
Although some studies have investigated the potential role of cats as a reservoir for Leishmania, their role in the epidemiology of visceral leishmaniasis (VL) is still poorly understood. Molecular diagnostic techniques are an important tool in VL diagnosis, and PCR shows high sensitivity and specificity for Leishmania spp. detection. Quantitative real-time PCR (qPCR) is a method that permits quantitative analysis of a large number of samples, resulting in more sensitive, accurate, and reproducible measurements of specific DNA present in the sample...
April 21, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28438213/cervid-herpesvirus-2-and-not-moraxella-bovoculi-caused-keratoconjunctivitis-in-experimentally-inoculated-semi-domesticated-eurasian-tundra-reindeer
#15
Morten Tryland, Javier Sánchez Romano, Nina Marcin, Ingebjørg Helena Nymo, Terje Domaas Josefsen, Karen Kristine Sørensen, Torill Mørk
BACKGROUND: Infectious keratoconjunctivitis (IKC) is a transmissible disease in semi-domesticated Eurasian reindeer (Rangifer tarandus tarandus). It is regarded as multifactorial and a single causative pathogen has not yet been identified. From clinical outbreaks we have previously identified Cervid herpesvirus 2 (CvHV2) and Moraxella bovoculi as candidates for experimental investigations. Eighteen reindeer were inoculated in the right eye with CvHV2 (n = 5), M. bovoculi (n = 5), CvHV2 and M...
April 24, 2017: Acta Veterinaria Scandinavica
https://www.readbyqxmd.com/read/28438142/%C3%AE-molecular-epidemiological-analysis-of-adenoviruses-from-excess-conjunctivitis-cases
#16
A Balasopoulou, P Κokkinos, D Pagoulatos, P Plotas, O E Makri, C D Georgakopoulos, A Vantarakis
BACKGROUND: Τo perform a molecular epidemiological analysis of viral conjunctivitis among excess conjunctivitis cases recorded at the University Hospital of Patras, Greece, for the period March to June 2012. METHODS: A structured questionnaire containing demographic and clinical data was developed in order to collect retrospective data on the cases. Eye swab specimens were collected and molecular detection of adenoviruses was performed by nested PCR. Positive results were confirmed by sequencing...
April 24, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28435821/utilization-of-pfge-as-a-powerful-discriminative-tool-for-the-investigation-of-genetic-diversity-among-mrsa-strains
#17
Solmaz Ohadian Moghadam, Mohammad Reza Pourmand, Masoumeh Douraghi, Samira Sabzi, Parisa Ghaffari
BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant challenge to the burn patient. The implementation of proper monitoring programs and prompt identification of epidemic MRSA strains are critical to consequently control and eradicate potential outbreaks. This study aimed to define the genetic relatedness of MRSA strains isolated from burn patients by analyzing the large fragments of DNA. METHODS: In this cross-sectional study, 126 pus/wound swabs from skin and soft tissue infections (SSTIs) were collected from inpatients of Shahid Motahari Burn Center (Tehran, Iran) in 2013...
March 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28435190/prevalence-of-salmonella-in-poultry-processing-environments-in-wet-markets-in-penang-and-perlis-malaysia
#18
Hafiz Nidaullah, Nadarajan Abirami, Ahamed Kamal Shamila-Syuhada, Li-Oon Chuah, Huda Nurul, Teik Pei Tan, Farah Wahida Zainal Abidin, Gulam Rusul
AIM: The aim of this study was to determine the prevalence of various Salmonella serotypes in chickens, carcass contact surfaces as well as environmental samples collected from wet markets and small scale processing plant. MATERIALS AND METHODS: A total of 182 poultry and environmental samples were collected at random on separate occasions from wet markets and small scale processing plant, during the period of October 2014 to July 2015 in Penang and Perlis, Malaysia...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28434680/inhibition-of-denture-plaque-by-tio2-coating-on-denture-base-resins-in-the-mouth
#19
Tomokuni Obata, Takayuki Ueda, Kaoru Sakurai
STATEMENT OF PROBLEM: Information is lacking about antiadhesion effect of titanium dioxide (TiO2) coating on denture base resins in the mouth. PURPOSE: The purpose of this clinical study was to investigate the antiadhesion effect of TiO2 coating on denture base resins. MATERIAL AND METHODS: Ten healthy dentate participants (mean 27 ±2 years of age) participated in this study. Disks and palatal appliances were made with denture base resin...
April 20, 2017: Journal of Prosthetic Dentistry
https://www.readbyqxmd.com/read/28433992/the-aachen-minipig-phenotype-genotype-hematological-and-biochemical-characterization-and-comparison-to-the-g%C3%A3-ttingen-minipig
#20
Kerstin Pawlowsky, Lisa Ernst, Julia Steitz, Thaddäus Stopinski, Babette Kögel, Anna Henger, Reinhart Kluge, René Tolba
BACKGROUND: The pig is one of the most frequently used large animal models for biomedical research, especially in the field of translational research and surgical models. While standard livestock breeds are used in short-term and acute studies, minipig breeds are the preferred breeds in long-term and chronic studies due to their limited growth and body weight. OBJECTIVE: In consideration of the 3R principle (refinement, reduction, replacement) and the increasing demand, the aim of this study was to generate a new, robust, non-specific-pathogen-free minipig breed, the Aachen minipig...
April 22, 2017: European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes
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