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C O'Connor, M G Kiernan, C Finnegan, J Powell, L Power, N H O'Connell, C P Dunne
BACKGROUND: The Mid-West of Ireland has higher than average national rates of invasive extended-spectrum beta-lactamase (ESBL) bloodstream infections and carbapenemase-producing Enterobacteriaceae (CPE), with increasing numbers of ESBL isolates detected in community-dwelling patients. AIMS: To conduct a point prevalence study in a convenience sample of the Mid-West population with the aim of determining the extent of ESBL colonisation. METHODS: Utilising anonymised community stool samples that had completed routine analysis, we conducted a point prevalence study over a 4-week period on all samples that met defined inclusion and exclusion criteria...
September 24, 2016: Irish Journal of Medical Science
Jeong Hyun Kim, Seung Min Yoo, Yong Hak Sohn, Chan Hee Jin, Yun Suk Yang, In Taek Hwang, Kwan Young Oh
OBJECTIVES: To investigate the predominant Lactobacillus species types of vaginal microbiota in pregnant Korean women by quantifying five Lactobacillus species and two anaerobes. METHODS: One hundred sixty-eight pregnant Korean women under antenatal care at Eulji University Hospital and local clinics were enrolled in the prospective cohort study during pregnancy (10-14 wks). Vaginal samples were collected with Eswab for qPCR and stored in a -80ºC freezer. Quantitative polymerase chain reaction was performed for five Lactobacillus species, and two anaerobes...
October 19, 2016: Journal of Maternal-fetal & Neonatal Medicine
C O'Connor, M G Kiernan, C Finnegan, M O'Hara, L Power, N H O'Connell, C P Dunne
Rapid detection of patients with carbapenemase-producing Enterobacteriaceae (CPE) is essential for the prevention of nosocomial cross-transmission, allocation of isolation facilities and to protect patient safety. Here, we aimed to design a new laboratory work-flow, utilizing existing laboratory resources, in order to reduce time-to-diagnosis of CPE. A review of the current CPE testing processes and of the literature was performed to identify a real-time commercial polymerase chain reaction (PCR) assay that could facilitate batch testing of CPE clinical specimens, with adequate CPE gene coverage...
August 17, 2016: Bioengineered
Alexander H Dalpke, Marjeta Hofko, Stefan Zimmermann
Vancomycin-resistant enterococci (VRE) are an important cause of health care-associated infections, resulting in significant mortality and a significant economic burden in hospitals. Active surveillance for at-risk populations contributes to the prevention of infections with VRE. The availability of a combination of automation and molecular detection procedures for rapid screening would be beneficial. Here, we report on the development of a laboratory-developed PCR for detection of VRE which runs on the fully automated Becton Dickinson (BD) Max platform, which combines DNA extraction, PCR setup, and real-time PCR amplification...
September 2016: Journal of Clinical Microbiology
Giovanni Cagnoni, Sara Giordana Rimoldi, Cristina Pagani, Claudio Savi, Fabrizio Stefani, Roberta Terzi, Pietro Olivieri, Giulia Tosi, Carlo Parravicini, Annamaria Di Gregorio, Carlo Antona, Maria Rita Gismondo
BACKGROUND: In 2015 a new device for the collection of mediastinal fluid from patients with deep sternal wound infection (DSWI) in the presence of negative-pressure wound therapy (NPWT) became available. The present study was designed to evaluate whether changing sample collection devices increased micro-organism detection in patients undergoing NPWT. METHODS: During 2013-2014, 207 samples were collected and cultured from NPWT patients (n = 23) to demonstrate the presence of DSWI using reticulated polyurethane sponge culture, a swab, and blood culture...
October 2016: Surgical Infections
Eric T Beck, Blake W Buchan, Garrett C Reymann, Nathan A Ledeboer
Paired nasal swab specimens were collected from patients who were undergoing routine methicillin-resistant Staphylococcus aureus (MRSA) screening prior to elective cardiac or orthopedic procedures. Each patient was swabbed using a traditional wound fiber liquid Stuart swab and an ESwab device, a flocked swab with a modified liquid Amies microbiology transport medium. The two specimens were tested using the Cepheid Xpert SA Nasal Complete assay. Results demonstrated a 95.5% agreement between the ESwab and the FDA-cleared wound fiber swab collection device...
July 2016: Journal of Clinical Microbiology
Suzane Silbert, Talita T Rocchetti, Alicia Gostnell, Carly Kubasek, Raymond Widen
Group B Streptococcus detection directly from Copan ESwab collected samples, using the BD Max GBS assay, was evaluated on receipt in the laboratory and after 24 h at room temperature. Results were compared to those using Lim broth enrichment PCR and culture. No significant difference was observed between 24 h ESwab and Lim broth PCRs.
June 2016: Journal of Clinical Microbiology
Kerin L Tyrrell, Diane M Citron, Eliza S Leoncio, Ellie J C Goldstein
We compared the eSwab system to a swab with an anaerobic transport semisolid agar system for their capacities to maintain the viability of 20 species of fastidious anaerobes inoculated on the bench and held at ambient or refrigerator temperature for 24 or 48 h. On average, both systems maintained similar viabilities among analogous groups of organisms at both temperatures, although there were quantitative differences among some species.
May 2016: Journal of Clinical Microbiology
Monica Yu, Karen Cross, Astrid Petrich, Joel Fish
PURPOSE: Any opening in a medical bed mattress cover may allow bodily fluids to enter the mattress, leading to contamination and potential nosocomial infection. This study's purpose was to assess permeability of crib mattress covers and measure bacterial growth within and on crib mattress surfaces. METHOD: Mattresses were selected randomly from hospital inventory. Bonney's blue dye was applied over mattress covers to assess permeability. Mattress cover surface swabs were acquired from standardized locations...
July 1, 2016: American Journal of Infection Control
Arlo Upton, Liselle Bissessor, Elizabeth Farrell, Stanford T Shulman, Xiaotian Zheng, Diana Lennon
Group A streptococcal (GAS) pharyngitis is a particularly important condition in areas of New Zealand where the incidence of acute rheumatic fever remains unacceptably high. Prompt diagnosis and treatment of GAS pharyngitis are cornerstones of the Rheumatic Fever Prevention Programme, but these are hindered by the turnaround time of culture. Tests with excellent performance and rapid turnaround times are needed. For this study, throat swabs (Copan ESwabs) were collected from schoolchildren self-identifying with a sore throat...
January 2016: Journal of Clinical Microbiology
Joakim Forsell, Satu Koskiniemi, Ida Hedberg, Helén Edebro, Birgitta Evengård, Margareta Granlund
Although PCR offers the potential for sensitive detection of parasites there are several pitfalls for optimal performance, especially when DNA is extracted from a complex sample material such as stool. With the aid of a sensitive inhibitor control in a duplex real-time PCR (qPCR) for identification of Entamoeba histolytica and Entamoeba dispar we have evaluated factors that influenced the performance of the qPCR and have suggested a rationale to be used in the analysis of clinical samples. Pre-PCR processing was found to be of outmost importance for an optimal amplification since inhibitors caused false-negative results when higher amounts of sample were used...
September 2015: Journal of Medical Microbiology
Stijn Jonckheere, Kristien Van Vaerenbergh, An Boel, Anne Vankeerberghen, Hans De Beenhouwer
The performance of the Xpert MRSA Gen 3 was compared to the Xpert MRSA on pooled eSwab media from nose, throat, and perineum using broth enriched cultured as gold standard. A lower specificity was found for the Xpert MRSA Gen 3 compared to the Xpert MRSA (91.8% versus 97.9%; P<0.05).
November 2015: Diagnostic Microbiology and Infectious Disease
C Bouchiat, M Bes, C Bouveyron, F Vandenesch, A Tristan
Staphylococcus aureus Panton-Valentine leukocidin (PVL) is associated with primary skin and soft-tissue infections (SSTI). We aimed to divert the RIDA®GENE PVL kit (RBiopharm) from its intended use on cultures to the detection of PVL-encoding genes directly from pus samples. Performance was compared with that of the in-house PCR method developed by the French National Reference Centre for Staphylococci. From June 2013 to May 2014, pus samples from S. aureus SSTI were tested. Our in-house PCR was performed on parallel cultures as the gold standard, while the RIDA®GENE PVL assay was used directly on pus samples from the sterile container, or a swab or an Eswab previously dipped in the pus...
September 2015: European Journal of Clinical Microbiology & Infectious Diseases
Suzane Silbert, Carly Kubasek, Faris Galambo, Elaine Vendrone, Raymond Widen
The BD Max MRSAXT and the BD Max StaphSR assays were validated for the detection of methicillin-resistant Staphylococcus aureus (MRSA) in ESwab samples. In addition, the BD Max StaphSR assay was evaluated for its ability to detect and differentiate S. aureus and MRSA in the same sample. A total of 255 ESwab samples collected from the anterior nares of patients were tested by each of three BD Max assays, including the BD Max MRSA first-generation assay. The results were compared to those of direct and enrichment culture...
August 2015: Journal of Clinical Microbiology
Manuela Avolio, Alessandro Camporese
We compared two types of liquid-based microbiology devices for microorganism viability according to standardized quantitative elution method CLSI M40-A2. The eSwab® met CLSI acceptance criteria of viability maintenance for all microorganisms tested. The Σ-Transwab® failed to meet CLSI acceptance criteria for Peptostreptococcus anaerobius, Prevotella melaninogenica, Fusobacterium nucleatum and Haemophilus influenzae.
August 2015: Journal of Microbiological Methods
Carolien M Wind, Henry J C de Vries, Maarten F Schim van der Loeff, Magnus Unemo, Alje P van Dam
Nucleic acid amplification tests (NAATs) are recommended for the diagnosis of N. gonorrhoeae infections because of their superior sensitivity. Increasing NAAT use causes a decline in crucial antimicrobial resistance (AMR) surveillance data, which rely on culture. We analyzed the suitability of the ESwab system for NAAT diagnostics and deferred targeted N. gonorrhoeae culture to allow selective and efficient culture based on NAAT results. We included patients visiting the STI Clinic Amsterdam, The Netherlands, in 2013...
June 2015: Journal of Clinical Microbiology
R Trebbien, B Andersen, J Rønn, J McCauley, T Kølsen Fischer
Although the ESwab kit (Copan, Brescia, Italy) is intended for sampling bacteria for culture, this kit is increasingly also used for virus sampling. The effect of ESwab medium on influenza virus detection by real-time reverse transcription-polymerase chain reaction (RT-PCR) or virus propagation in Madin-Darby canine kidney (MDCK) cell culture was investigated. The ESwab medium was suitable for viral RNA detection but not for viral propagation due to cytotoxicity. Sampling influenza viruses with ESwab challenges influenza surveillance by strongly limiting the possibility of antigenic characterisation...
2014: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
Susanna Esposito, Leonardo Terranova, Alberto Zampiero, Valentina Ierardi, Walter Peves Rios, Claudio Pelucchi, Nicola Principi
BACKGROUND: As healthy children are the main reservoir of respiratory pathogens and the main cause of bacterial diffusion in the community, it could be interesting to investigate the type of screening that should be used during the early years of life in order to obtain a more precise estimate of Staphylococcus aureus circulation. The aim of this study was to evaluate oropharyngeal and nasal S. aureus carriage in otherwise healthy children and adolescents aged 6-17 years. METHODS: The oropharyngeal and nasal samples were collected in December 2013 from 497 healthy students attending five randomly selected schools in Milan, Italy, using an ESwab kit, and S...
2014: BMC Infectious Diseases
Kaivon Pakzad-Vaezi, Steve D Levasseur, Steven Schendel, Sean Mark, Richard Mathias, Diane Roscoe, Simon P Holland
PURPOSE: To determine if a new, single-sample device (ESwab; Copan Diagnostics, Inc) can simplify the traditional multi-sample approach to specimen collection in infectious keratitis. DESIGN: Prospective, diagnostic test evaluation. METHODS: In this institutional study, patients with suspected infectious keratitis meeting traditional criteria for diagnostic corneal specimen collection and culture were randomized to the order of first specimen collection method: ESwab or a sample directly plated for growth on chocolate agar...
January 2015: American Journal of Ophthalmology
Alexander H Dalpke, Marjeta Hofko, Christian Stock, Stefan Zimmermann
We evaluated the performance of the BD Max MRSA XT assay for use with different swab types. The 90% detection rates (95% confidence intervals) were 387 (97 to 1,551), 877 (238 to 3,230), 986 (183 to 5,287), 1,292 (328 to 5,078), 2,400 (426 to 13,518), and 5,848 (622 to 55,021) CFU/swab for Liquid Stuart, Liquid Amies, dry, Amies Gel without charcoal, ESwab collection, and Amies gel with charcoal swabs (Becton Dickinson), respectively. Amies Gel without charcoal, ESwab collection, and Amies gel with charcoal swabs had a tendency to be less sensitive, but none of the differences was statistically significant...
December 2014: Journal of Clinical Microbiology
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