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Moritz K F Wolf, Aurélie Closet, Monika Bzowska, Jean-Marc Bielser, Jonathan Souquet, Hervé Broly, Massimo Morbidelli
Mammalian cell perfusion cultures represent a promising alternative for the production of various biopharmaceuticals to the current fed-batch technology. Long-term operation at a fixed viable cell density (VCD) requires a viable culture and a constant removal of excessive cells. Product loss in the cell removing bleed stream deteriorates the process yield. In this study, we investigated the use of chemical and environmental growth inhibition on culture performance by either adding valeric acid (VA) to the production media or by reducing the culture temperature (33...
May 21, 2018: Biotechnology Journal
Sun-Ji Park, Jung-Hak Kim, Dong Gil Lee, Jin-Man Kim, Dong-Seok Lee
Reactive oxygen species (ROS) produced in biological reactions have been shown to contribute to ovarian aging. Peroxiredoxin 2 (Prx2) is an antioxidant enzyme that protects cells by scavenging ROS; however, its effect on age-related, oxidative stress-associated ovarian failure has not been reported. Here, we investigated its role in age-related ovarian dysfunction and 4-vinylcyclohexene diepoxide (VCD)-induced premature ovarian failure using Prx2-deficient mice. Compared to those in wildtype (WT) mice, serum levels of anti-Müllerian hormone, 17β-estradiol, and progesterone and numbers of follicles and corpora lutea were significantly lower in 18-month-old Prx2-/- mice...
May 17, 2018: Free Radical Biology & Medicine
Joanna Ewa Rode, Krzysztof Lyczko, Magdalena Jawiczuk, Robert Kawęcki, Wojciech Stańczyk, Agnieszka Jaglińska, Jan Cz Dobrowolski
The IR and VCD spectra of both enantiomers of Me, iPr, nBu, Ph and CH2Ph substituted isoindolinones in solution and KBr pellets were measured and interpreted with DFT calculations. The spectra in solution revealed no important differences in the C=O stretching vibration region while the interpretation of very distinct spectra taken in pellets required determining the crystal structures. The studied compounds crystallized in the P212121 (Me, iPr, CH2Ph), P31 (nBu), and P21 (Ph) space groups. We found that the quality of simulated spectra strongly depends on the substituent, the structure of the molecular cluster assumed, basis set, and use of the dispersion correction...
May 18, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Katia Le Barbu-Debus, Arne Scherrer, Aude Bouchet, Daniel Sebastiani, Rodolphe Vuilleumier, Anne Zehnacker
The influence of flexibility and hydrogen bond formation on the IR absorption and vibrational circular dichroism (VCD) spectrum of a floppy protic molecule, namely, (S)-1-indanol, is studied in both non-polar CCl4 and polar DMSO solvents. The experimental IR absorption and VCD spectra obtained by Fourier transform spectroscopy are interpreted using both static density functional theory (DFT) calculations and first principles molecular dynamics (FPMD) within DFT, using the nuclear velocity perturbation theory (NVPT)...
May 17, 2018: Physical Chemistry Chemical Physics: PCCP
Yunjie Xu, Angelo Shehan Perera, Joseph Cheramy, Christian Merten, Javix Thomas
Solvent effects, in particular those involving water as the solvent, are of significant interest to chemistry and physics communities. IR, vibrational circular dichroism (VCD), Raman, and Raman optical activity (ROA) spectra of methyl glycidate in two very different solvents, namely CCl4 and water, have been measured experimentally and simulated theoretically. While the observed spectra in CCl4 could be well modelled using the polarizable continuum model for the solvent, the situation is much different in water...
May 16, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Nader Mankerious, Katharina Mayer, Senta M Gewalt, Sandra M Helde, Tareq Ibrahim, Lorenz Bott-Flügel, Karl-Ludwig Laugwitz, Heribert Schunkert, Adnan Kastrati, Stefanie Schüpke
OBJECTIVES: To assess the safety and efficacy of arteriotomy closure with the intravascular FemoSeal vascular closure device (VCD; St. Jude Medical) compared to manual compression in patients undergoing diagnostic cardiac catheterization via the common femoral artery. BACKGROUND: There is limited evidence on the performance of individual contemporary VCDs compared to manual compression. METHODS: This is a subanalysis of 3018 patients who underwent transfemoral diagnostic coronary angiography and were randomly assigned to arteriotomy closure with either the intravascular FemoSeal VCD or manual compression within the investigator-initiated, large-scale, randomized, multicenter, open-label ISAR-CLOSURE trial...
May 15, 2018: Journal of Invasive Cardiology
Maria Giovanna Lizio, Valery Andrushchenko, Sarah J Pike, Anna D Peters, George F S Whitehead, Iñigo J Vitórica-Yrezábal, Shaun T Mutter, Jonathan Clayden, Petr Bouř, Ewan W Blanch, Simon John Webb
Helical α-aminoisobutyric acid (Aib) foldamers show great potential as devices for the communication of conformational information across phospholipid bilayers, but determining their conformation in bilayers remains a challenge. In the present study, Raman, Raman optical activity (ROA), infrared (IR) and vibrational circular dichroism (VCD) spectroscopies have been used to analyze the conformational preferences of Aib foldamers in solution and when interacting with bilayers. A 310-helix marker band at 1665-1668 cm-1 in Raman spectra was used to show that net helical content increased strongly with oligomer length...
May 10, 2018: Chemistry: a European Journal
Tao Long, Jeffrey K Yao, Junyi Li, Ziv Z Kirshner, Doug Nelson, George G Dougherty, Robert B Gibbs
Loss of ovarian function has important effects on neurotransmitter production and release with corresponding effects on cognitive performance. To date, there has been little direct comparison of the effects of surgical and transitional menopause on neurotransmitter pathways in the brain. In this study, effects on monoamines, monoamine metabolites, and the amino acids tryptophan (TRP) and tyrosine (TYR) were evaluated in adult ovariectomized (OVX) rats and in rats that underwent selective and gradual ovarian follicle depletion by daily injection of 4-vinylcyclohexene-diepoxide (VCD)...
May 5, 2018: Molecular and Cellular Endocrinology
Anne E Vertigan, Sarah M Kapela, Emily K Kearney, Peter G Gibson
BACKGROUND: Chronic refractory cough(CRC), a phenotype of Cough Hypersensitivity Syndrome(CHS), is a disabling problem. Laryngeal dysfunction may be important in CC and CHS because laryngeal symptoms are common and CC, however the role of laryngeal dysfunction in CHS has not been systematically examined. OBJECTIVE: To determine the nature of laryngeal dysfunction in patients with CC and compare with the related laryngeal conditions of Vocal Cord Dysfunction(VCD) and Muscle Tension Dysphonia(MTD)...
May 2, 2018: Journal of Allergy and Clinical Immunology in Practice
Hari Bogabathina, Runhua Shi, Sampath Singireddy, Liam Morris, Abdulrahman Abdulbaki, Henock Zabher, Pavan Katikaneni, Kalgi Modi
BACKGROUND: Femoral arterial access (FAA) during diagnostic coronary angiography (CAG) and percutaneous coronary interventions (PCI) are associated with several vascular complications (VC). VC rate in our experience a decade ago was 3.02% and higher in women (4.7% in women, and 1.67% in men, p < 0.0006), with an OR of 2.81 (95% CI: 1.51-5.22). METHODS: Patients who underwent CAG and PCI utilizing FAA (n = 2617) were separated into Period 1 (2005 to 2008; 1970 patients; Male 1045; Female 925) and Period 2 (2016-2017; 647 patients; Male 357; Female 290)...
April 3, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
Tiziana Benincori, Giulio Appoloni, Patrizia Romana Mussini, Serena Arnaboldi, Roberto Cirilli, Elsa Quartapelle Procopio, Monica Panigati, Sergio Abbate, Giuseppe Mazzeo, Giovanna Longhi
Two new inherently chiral oligothiophenes characterized by the atropisomeric 3,3'-bithianaphtene scaffold functionalized with fused ring bithiophene derivatives, namely 4H-cyclopenta [2,1-b3:4b']dithiophene (CPDT) and dithieno[3,3-b:2',3'-d]pyrrole (DTP), were synthesized. The racemates were fully characterized and resolved into antipodes by enantioselective HPLC. The enantiomers were analyzed through different chiroptical techniques: electronic circular dichroism (ECD) and vibrational circular dichroism (VCD) were employed to attribute the absolute configuration (AC)...
May 2, 2018: Chemistry: a European Journal
Maria Buchsteiner, Lake-Ee Quek, Peter Gray, Lars K Nielsen
Lactate is one of the key waste metabolites of mammalian cell culture. High lactate levels are caused by high aerobic glycolysis, also known as the Warburg effect, and are usually associated with adverse culture performance. Therefore, reducing lactate accumulation has been an ongoing challenge in cell culture development in order to improve growth, productivity and process robustness. The pyruvate dehydrogenase complex (PDC) plays a crucial role for the fate of pyruvate, as it converts pyruvate to acetyl-CoA...
April 28, 2018: Biotechnology and Bioengineering
Niranjan V Ilawe, Reinhard Schweitzer-Stenner, David DiGuiseppi, Bryan M Wong
Short peptides have emerged as versatile building blocks for supramolecular structures and hydrogels. In particular, the presence of aromatic amino acid residues and/or aromatic end groups is generally considered to be a prerequisite for initiating aggregation of short peptides into nanotubes or cross β-sheet type fibrils. However, the cationic GAG tripeptide surprisingly violates these rules. Specifically, in water/ethanol mixtures, GAG peptides aggregate into very long crystalline fibrils at temperatures below 35 °C where they eventually form a spanning network structure and, thus, a hydrogel...
April 26, 2018: Physical Chemistry Chemical Physics: PCCP
Hari Bogabathina, Sampath Singireddy, Runhua Shi, Liam Morris, Abdulrahman Abdulbaki, Henock Zabher, Pavan Katikaneni, Kalgi Modi
BACKGROUND: Micropuncture technique (MPT) is being adapted nationally to reduce vascular complications (VC). We initiated a quality improvement project in our cath lab to reduce VCs utilizing MPT. METHODS: We utilized MPT on all of our non-STEMI femoral artery (FA) access cases starting September 2016. As a comparator group, we collected data from April to August 2016. Anatomic localization of FA and fluoroscopic marking of femoral head was utilized in all cases...
March 26, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
Fausto Biancari, Hannu Romppanen, Mikko Savontaus, Antti Siljander, Timo Mäkikallio, Olli-Pekka Piira, Jarkko Piuhola, Viivi Vilkki, Antti Ylitalo, Tuija Vasankari, Juhani K E Airaksinen, Matti Niemelä
BACKGROUND: The MANTA system is a novel vascular closure device (VCD) and its safety and efficacy were compared to the ProGlide VCD in patients undergoing transfemoral transcatheter aortic valve implantation (TAVI). METHODS: This is a retrospective study including 222 patients who underwent transfemoral TAVI at three Finnish University Hospitals. The MANTA VCD was used in 107 patients and their outcome was compared with that of 115 patients in whom the arterial access was closed with the ProGlide VCD...
April 14, 2018: International Journal of Cardiology
Roberto Gabrielli, Maria Sofia Rosati, Massiliano Millarelli, Angelica Dante, Maurizio Maiorano, Aldo Musilli, Roberto Chiappa, Marco Ventura
BACKGROUND: Common femoral artery is still the most frequently site used for vascular access, mostly for peripheral arterial interventions and its puncture remains a significant source of patient's morbidity. Manual compression (MC) has been the gold standard for hemostasis after femoral catheterization until recently, but only in the last few years, vascular closure devices (VCDs) are replacing MC due to their rapid development. Nowadays also vascular surgeons are becoming familiar with VCDs...
April 17, 2018: Annals of Vascular Surgery
Belen Nieto-Ortega, Rafael Rodríguez, Samara Medina Rivero, Emilio Quiñoá, Ricardo Riguera, Juan Casado Cordón, Félix Freire, Francisco Javier Ramírez
Several steps of chiral induction have been detected in poly(phenylacetylene)s among their different hierarchical levels of chirality by vibrational circular dichroism, namely: i) from the stereogenic centers to the innermost polyacetylene helical covalent backbone (helixint); ii) from this to the external helix (helixext) formed by the side phenyl pendants which forms a complementary helix or counter-helix, and iii) from this pendant helix to the helical solvation sphere (helixsolv.), being the last one observed along this work...
April 13, 2018: Journal of Physical Chemistry Letters
Leo A Joyce, Christopher C Nawrat, Edward C Sherer, Mirlinda Biba, Andrew Brunskill, Gary E Martin, Ryan D Cohen, Ian W Davies
This work describes the application of vibrational (VCD) and electronic (ECD) circular dichroism spectroscopy to solve the longstanding debate around the absolute configuration of (+)-frondosin B ( 1 ). The absolute configuration of (+)- 1 could confidently be assigned as ( R ) using these spectroscopic techniques. The discrepancy in the optical rotation (OR) values obtained in previous studies can be attributed to an undetected minor impurity ( ca. 7%) that arose unexpectedly in a key step late in the synthesis...
January 14, 2018: Chemical Science
Jinglei Yang, Miaozhen Pan, Peter S Reinach, Rongfang Chen, Lan Yu, Feng Zhou, Sen Zhang, Jia Qu, Xiangtian Zhou
Retinal arachidonic acid (ARA) levels in form-deprived eyes decline in guinea pigs. As prostaglandin F2α (PGF2α) is an ARA metabolite and endogenous agonist of prostaglandin F receptor (FP), we hypothesized that down-regulation of PGF2α-FP receptor signalling pathway contributes to myopia onset. To test this hypothesis, this study determines if: a) retinal PGF2α levels decline during development of form deprivation myopia (FDM) in guinea pigs; b) FP receptor agonism and antagonism alter emmetropization and myopia development...
March 31, 2018: Basic & Clinical Pharmacology & Toxicology
Tommaso Giovannini, Gianluca Del Frate, Piero Lafiosca, Chiara Cappelli
We present a computational methodology based on a polarizable Quantum Mechanical (QM)/Molecular Mechanics (MM) approach to accurately compute the Vibrational Optical Activity (VOA) spectra of chiral systems. This approach is applied for the calculation of Infrared (IR), Vibrational Circular Dichroism (VCD), Raman and Raman Optical Activity (ROA) spectra of aqueous solutions of (l)-methyl lactate and (S)-glycidol. Remarkable agreement between calculations and experiments is reported, showing the reliability and accuracy of the methodology, especially with respect to standard continuum solvation approaches...
March 21, 2018: Physical Chemistry Chemical Physics: PCCP
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