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https://www.readbyqxmd.com/read/28640131/paragonimiasis-in-children-in-southwest-china-a-retrospective-case-reports-review-from-2005-to-2016
#1
Zongrong Gong, Ruixue Miao, Min Shu, Yu Zhu, Yang Wen, Qin Guo, Qiong Liao, Chaomin Wan
BACKGROUND: Paragonimiasis infection has no specific symptoms or typical radiologic findings, leading to the possibility of misdiagnosis. Thus, the objective of this study was to analyze clinical and radiological features, and treatment outcome of paragonimiasis in children in Southwest China to improve the awareness of this disease. METHODS: We retrospectively reviewed the records of children diagnosed with paragonimiasis in West China Second University Hospital between 2005 and 2016...
June 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28639565/diagnostic-performance-of-the-genotype-mtbdrplus-and-mtbdrsl-assays-to-identify-tuberculosis-drug-resistance-in-eastern-china
#2
Qiao Liu, Guo-Li Li, Cheng Chen, Jian-Ming Wang, Leonardo Martinez, Wei Lu, Li-Mei Zhu
BACKGROUND: The WHO recently has recommended the GenoType MTBDRplus version 1.0 and MTBDRsl version 1.0 assays for widespread use in countries endemic with drug-resistant tuberculosis. Despite this, these assays have rarely been evaluated in China, where the burden of drug-resistant tuberculosis is among the highest globally. METHODS: Mycobacterium tuberculosis clinical isolates were obtained between January 2008 and December 2008. Isolates were tested for drug resistance against rifampicin (RFP) and isoniazid (INH) using the GenoType MTBDRplus assay and drug resistance against ethambutol (EMB), ofloxacin (OFX), and kanamycin (KM) using the Genotype MTBDRsl assay...
July 5, 2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28638662/comparison-of-dna-extraction-protocols-and-molecular-targets-to-diagnose-tuberculous-meningitis
#3
Flavia Silva Palomo, Martha Gabriela Celle Rivero, Milene Gonçalves Quiles, Fernando Pereira Pinto, Antonia Maria de Oliveira Machado, Antonio Carlos Campos Pignatari
Tuberculous meningitis (TBM) is a severe form of extrapulmonary tuberculosis. The aims of this study were to evaluate in-house molecular diagnostic protocols of DNA extraction directly from CSF samples and the targets amplified by qPCR as an accurate and fast diagnosis of TBM. One hundred CSF samples from 68 patients suspected of TBM were studied. Four DNA extraction techniques (phenol-chloroform-thiocyanate guanidine, silica thiocyanate guanidine, resin, and resin with ethanol) were compared and CSF samples were used to determine the best target (IS6110, MPB64, and hsp65 KDa) by qPCR...
2017: Tuberculosis Research and Treatment
https://www.readbyqxmd.com/read/28633709/primary-pulmonary-actinomycosis-a-retrospective-analysis-of-145-cases-in-mainland-china
#4
M Zhang, X-Y Zhang, Y-B Chen
OBJECTIVE: To improve our understanding of pulmonary actinomycosis. METHODS: A retrospective analysis of 145 cases in mainland China was conducted. RESULTS: The male:female ratio was 2.7:1. Mean age at diagnosis was 48 years (± 12). Main symptoms were cough (87.6%), expectoration (40%), blood-stained sputum (37.2%), fever (26.9%), chest pain (24.8%) and haemoptysis (16.6%). Eighty-eight (60.7%) patients had no underlying disease. Only five patients received a correct initial diagnosis; 60 patients were misdiagnosed with lung cancer, followed by pulmonary tuberculosis (TB) and lung abscess...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28633707/qualitative-analysis-of-biological-tuberculosis-samples-by-an-electronic-nose-based-artificial-neural-network
#5
E I Mohamed, M A Mohamed, M H Moustafa, S M Abdel-Mageed, A M Moro, A I Baess, S M El-Kholy
OBJECTIVE: To apply an e-nose system for monitoring headspace volatiles in biological samples from Egyptian patients with active pulmonary tuberculosis (TB) and healthy controls (HCs) and compare them with standard sputum analysis. DESIGN: The study population comprised 260 (140 males, 120 females) newly diagnosed TB patients and 240 (120 males, 120 females) HCs matched by age and socio-economic level admitted to hospitals specialising in chest diseases in Alexandria, Behera, Giza and Damietta Governorates, Egypt...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28633699/migration-histories-of-multidrug-resistant-tuberculosis-patients-from-the-thailand-myanmar-border-2012-2014
#6
S S Thi, D M Parker, L L Swe, S Pukrittayakamee, C L Ling, K Amornpaisarnloet, M Vincenti-Delmas, F H Nosten
SETTING: Multidrug-resistant tuberculosis (MDR-TB) is a growing public health threat in South-East Asia. TB is typically a disease of poverty and can be spread by infectious humans who migrate from one region to another. DESIGN: We interviewed 20 MDR-TB patients on the Thailand-Myanmar border with regard to their migration histories. Migration origins and destinations were mapped. RESULTS: All but one participant had a history of migration, and maps of migration ranges revealed wide geographic dispersal...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28633698/feasibility-of-a-streamlined-tuberculosis-diagnosis-and-treatment-initiation-strategy
#7
P B Shete, T Nalugwa, K Farr, C Ojok, M Nantale, P Howlett, P Haguma, E Ochom, F Mugabe, M Joloba, L H Chaisson, D W Dowdy, D Moore, J L Davis, A Katamba, A Cattamanchi
OBJECTIVE: To assess the feasibility of a streamlined strategy for improving tuberculosis (TB) diagnostic evaluation and treatment initiation among patients with presumed TB. DESIGN: Single-arm interventional pilot study at five primary care health centers of a streamlined, SIngle-saMPLE (SIMPLE) TB diagnostic evaluation strategy: 1) examination of two smear results from a single spot sputum specimen using light-emitting diode fluorescence microscopy, and 2) daily transportation of smear-negative sputum samples to Xpert® MTB/RIF testing sites...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28633696/cross-border-collaboration-for-improved-tuberculosis-prevention-and-care-policies-tools-and-experiences
#8
M Dara, G Sulis, R Centis, L D'Ambrosio, G de Vries, P Douglas, D Garcia, N Jansen, E Zuroweste, G B Migliori
As tuberculosis (TB) spreads beyond borders with people movements, several interventions ensuring the continuity of care are essential, although difficult to put in place in the absence of well-defined agreements allowing data sharing and easy referral of patients to appropriate health facilities. This article first sets out general principles for cross-border collaboration and continuity of care. It then presents a series of case studies. Policies and practices on cross-border collaboration in selected low-incidence countries (Australia, Italy, Norway, The Netherlands, the United Kingdom and the United States) are described and critically appraised...
July 1, 2017: International Journal of Tuberculosis and Lung Disease
https://www.readbyqxmd.com/read/28633546/identification-of-specific-antibodies-against-the-ag85c-mpt51-hspx-fusion-protein-cmx-for-serological-screening-of-tuberculosis-in-endemic-area
#9
Adrielle Zagmignan, Adeliane Castro da Costa, José Lima Viana, Lídio Gonçalves Lima Neto, Cristina de Andrade Monteiro, Ariosvaldo Guimarães Gaioso Neto, Ana Paula Junqueira Kipnis, Eduardo M de Sousa
OBJECTIVES: Development of new tools for rapid and accurate diagnosis of tuberculosis (TB) is considered a strategy for controlling the disease. The recombinant CMX fusion protein is composed of immunodominant epitopes of the Ag85C (Rv0129c), MPT51 (Rv3803c) and the entire HspX (Rv2031c) proteins from Mycobacterium tuberculosis H37Rv (Mtb). The aim of this study was to evaluate the applicability of a test using the CMX protein in individuals suspected of TB. METHODS: Indirect ELISA was used to measure serum anti-CMX IgM and IgG in individuals with pulmonary TB...
June 21, 2017: Expert Review of Clinical Immunology
https://www.readbyqxmd.com/read/28631909/-characterization-of-microbial-flora-in-patients-suspected-of-having-urogenital-tuberculosis
#10
A G Cherednichenko, E V Kulchavenya
INTRODUCTION: In the structure of tuberculosis morbidity, urogenital tuberculosis (UGT) ranks second or third only to pulmonary tuberculosis. Every fourth patient has the stage of cavern formation at first presentation. Seventy five percent of nephrotuberculosis cases co-occur with nonspecific pyelonephritis, which excludes "sterile pyuria". We aimed to determine the microbial spectrum of urine and gonadal appendages in patients with suspected UGT and to investigate the susceptibility of the isolated microorganisms to the main antibacterial drugs...
June 2017: Urologii︠a︡
https://www.readbyqxmd.com/read/28631643/multidrug-resistant-tuberculosis-detection-and-characterization-of-mutations-in-mycobacterium-tuberculosis-by-genotype-mtbdrplus
#11
Rajneesh Tripathi, Shampa Anupurba
Detection of drug resistance in Mycobacterium tuberculosis by conventional phenotypic drug susceptibility testing methods requires several weeks. Therefore, molecular diagnostic tests for rapid detection of multidrug resistance tuberculosis (MDR-TB) are urgently needed. Early diagnosis helps in initiating optimal treatment which would not only enable cure of an individual patient but also will curb the transmission of drug resistance in the community. Line probe assay (LPA) has shown great promises in the diagnosis of MDR-TB...
April 2017: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/28630849/a-case-of-chronic-inflammation-of-the-ankle-joint-with-subtle-signs-of-inflammation-a-rare-presentation-of-tuberculosis-ankle
#12
Jeevan Pereira, S Anoop, George Joy Pettah
INTRODUCTION: Tuberculosis (TB) of the ankle is an uncommon presentation of extrapulmonary infection. The early diagnosis of TB arthritis of the ankle is difficult because of the uncommon site, the ability of TB to mimic other disorders and, all too common, lack of awareness of about this infection in ankle joint. CASE REPORT: A 30-year-old male presented with a 1-year history of right ankle pain and swelling. He denied any history of recent infection but had had recurrent ankle pain that had been treated accordingly...
January 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28630838/a-case-report-of-an-unusual-case-of-tuberculous-osteomyelitis-causing-spontaneous-pathological-fracture-of-humerus-in-a-middle-aged-female
#13
Umesh Birole, Ashish Ranade, Mahesh Mone
INTRODUCTION: Tuberculosis is a major health problem worldwide. Extrapulmonary tuberculosis is often secondary to some primary foci in lungs. There are reports of tuberculous osteomyelitis involving maxilla, ulna, femur, and shoulder joint but none have reported pathological fracture in humeral diaphysis due to tuberculosis osteomyelitis without shoulder joint involvement. We report a case of pathological fracture of humerus diaphysis due to tuberculous osteomyelitis with normal articular space...
January 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28630834/van-neck-odelberg-disease-a-rare-case-report
#14
Amit Prakash Chaudhari, Gautam Shah, Sameer Sanjeev Patil, Aashish Babanrao Ghodke, Sagar B Kelkar
INTRODUCTION: Pain in groin without any history of trauma or positive clinical findings of infection or stress fracture in ischiopubic ramus poses challenges in diagnosis of the condition in prepubertal age. "Osteochondrosis of ischiopubic synchondrosis (IPS)" also called as "Van Neck-Odelberg" disease. Left leg dominant patient presented with pain in groin and limping with left leg dominance. Differential diagnosis - such as stress fractures, osteomyelitis, tuberculosis, post-traumatic osteolysis, or any neoplasia - is need be ruled out to arrive at diagnosis...
January 2017: Journal of Orthopaedic Case Reports
https://www.readbyqxmd.com/read/28630794/a-case-of-presumed-tuberculosis-uveitis-with-occlusive-vasculitis-from-an-endemic-region
#15
Berna Başarır, Yalçın Karaküçük, Çiğdem Altan, Banu Şatana, Bulut Ocak, Aslı İnal
In this report, we present a case with presumed unilateral tuberculosis uveitis from an endemic region. A 23-year-old male presented with decreased vision in his left eye for 15 days. Visual acuities were 1.0 in his right eye and 0.3 in his left eye. Ophthalmologic examination was normal for the right eye. Slit-lamp examination revealed 2+ cells in the vitreous without anterior chamber reaction in his left eye. Fundus examination revealed occlusive vasculitis and granuloma. His history revealed that he had a respiratory infection with fever 3 months ago while visiting his native country, Rwanda, and was treated with non-specific antibiotic therapy...
June 2017: Turkish Journal of Ophthalmology
https://www.readbyqxmd.com/read/28629765/study-on-the-relationship-between-vitamin-d-deficiency-and-susceptibility-to-spinal-tuberculosis
#16
Liang Tang, Shen Liu, Yu-Cheng Bao, Rui-Xiao Gao, Chen-Fu Han, Xiao-Chen Sun, Wen-Long Zhang, Shi-Qing Feng
OBJECTIVE: To investigate the effect of vitamin D deficiency on susceptibility to spinal tuberculosis and its pathological development. METHODS: A case-control design was used in this study. A total of 163 treatment-naïve patients with spinal tuberculosis admitted to this institute for an operation from June 2013 to May 2016 were included in the case group, and 170 subjects who received a health examination in the same hospital were included in the control group...
June 16, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28629614/molecular-detection-methods-of-resistance-to-antituberculosis-drugs-in-mycobacterium-tuberculosis
#17
F Brossier, W Sougakoff
OBJECTIVE: Molecular methods predict drug resistance several weeks before phenotypic methods and enable rapid implementation of appropriate therapeutic treatment. We aimed to detail the most representative molecular tools used in routine practice for the rapid detection of resistance to antituberculosis drugs among Mycobacterium tuberculosis strains. MATERIALS AND METHODS: The molecular diagnosis of resistance to antituberculosis drugs in clinical samples or from in vitro cultures is based on the detection of the most common mutations in the genes involved in the development of resistance in M...
June 16, 2017: Médecine et Maladies Infectieuses
https://www.readbyqxmd.com/read/28628760/tuberculosis-incidence-among-people-living-with-hiv-aids-with-virological-failure-of-antiretroviral-therapy-in-salvador-bahia-brazil
#18
Monaliza Cardozo Rebouças, Márcio Oliveira da Silva, Tatiana Haguihara, Carlos Brites, Eduardo Martins Netto
Antiretroviral therapy (ART) for HIV has led to increased survival of HIV-infected patients. However, tuberculosis (TB) remains the leading opportunistic infection and cause of death among people living with HIV/AIDS (PLWHA). TB has been shown to be a good predictor of virological failure among PLWHA. This study aimed to evaluate the incidence of TB and its consequences among individuals diagnosed with virological failure of HIV. This was a retrospective cohort study involving PLWHA being followed-up in an AIDS reference center in Salvador, Bahia, Brazil...
June 16, 2017: Brazilian Journal of Infectious Diseases
https://www.readbyqxmd.com/read/28628338/community-acquired-tsukamurella-pneumonia-in-a-young-immunocompetent-adult-a-case-misdiagnosed-as-pulmonary-tuberculosis-and-literature-review
#19
LiKun Yang, Yan Cao, Zijun Dan, Zhongyuan Wang, Xinjing Wang
This report describes a case of Tsukamurella pneumonia in a 24-year-old immunocompetent woman. The patient was diagnosed as having pulmonary tuberculosis and was treated for nearly 9 months. The correct diagnosis was suspected only when the results of mycobacteria nucleic acid amplification tests on cultural colonies were negative. Tsukamurella was identified from the patient's sputum and bronchoalveolar lavage fluid by using 16S rRNA sequence analysis. Here, the clinical presentation, diagnosis, and treatment strategies of Tsukamurella pneumonia are discussed, along with a literature review...
June 19, 2017: Postgraduate Medicine
https://www.readbyqxmd.com/read/28627685/metabolomic-analysis-based-on-1h-nuclear-magnetic-resonance-spectroscopy-metabolic-profiles-in-tuberculous-malignant-and-transudative-pleural-effusion
#20
Cheng Wang, Jingjin Peng, Yanling Kuang, Jiaqiang Zhang, Luming Dai
Pleural effusion is a common clinical manifestation with various causes. Current diagnostic and therapeutic methods have exhibited numerous limitations. By involving the analysis of dynamic changes in low molecular weight catabolites, metabolomics has been widely applied in various types of disease and have provided platforms to distinguish many novel biomarkers. However, to the best of our knowledge, there are few studies regarding the metabolic profiling for pleural effusion. In the current study, 58 pleural effusion samples were collected, among which 20 were malignant pleural effusions, 20 were tuberculous pleural effusions and 18 were transudative pleural effusions...
June 12, 2017: Molecular Medicine Reports
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