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https://www.readbyqxmd.com/read/28718696/tumor-induced-disorder-of-iron-metabolism-in-major-organs-a-new-insight-from-chemical-speciation-of-iron
#1
Rujie Chen, Guangcun Chen
Objective To investigate the evolution of iron speciation in major organs of tumor-bearing mice and its role in cancer formation and cancer-associated complications. Methods The concentration and chemical speciation of iron in the spleen, liver, lung, kidney, heart, blood, muscle, and tumor tissue of healthy mice and tumor-bearing mice were studied by synchrotron radiation-based total reflection X-ray fluorescence spectrometry (SR-TXRF) coupled with X-ray absorption spectroscopy (XAS). Results The TXRF and XAS results showed that the iron content, especially the ferritin content, significantly decreased in the blood and spleen but significantly increased in the liver, lung, and muscle of mice after tumor implantation...
January 1, 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28717144/radseq-provides-evidence-for-parallel-ecotypic-divergence-in-the-autotetraploid-cochlearia-officinalis-in-northern-norway
#2
Marie K Brandrud, Ovidiu Paun, Maria T Lorenzo, Inger Nordal, Anne K Brysting
Speciation encompasses a continuum over time from freely interbreeding populations to reproductively isolated species. Along this process, ecotypes - the result of local adaptation - may be on the road to new species. We investigated whether three autotetraploid Cochlearia officinalis ecotypes, adapted to different habitats (beach, estuary, spring), are genetically differentiated and result from parallel ecotypic divergence in two distinct geographical regions. We obtained genetic data from thousands of single nucleotide polymorphisms (SNPs) from restriction-site associated DNA sequencing (RADseq) and from six microsatellite markers for 12 populations to assess genetic divergence at ecotypic, geographic and population level...
July 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28717110/mercury-and-selenium-levels-and-their-molar-ratios-in-several-species-of-commercial-shrimp-in-japan-regarding-the-health-risk-of-methylmercury-exposure
#3
Van Anh Thi Hoang, Mineshi Sakamoto, Megumi Yamamoto
The Japanese shrimp industry depends on importing shrimp from other countries. However, little information is available on mercury speciation and selenium (Se) concentrations in commercial shrimp available in Japan. The present study determined the concentrations of total mercury (T-Hg), methylmercury (MeHg), and Se in the muscles (wet weight) of imported and domestic commercial shrimp from Kumamoto and Kagoshima prefectures to obtain information for assessing the risk of MeHg exposure. The median concentrations of T-Hg, MeHg and Se in shrimp imported from three different countries were, respectively: black tiger shrimp (n = 18), 15...
2017: Journal of Toxicological Sciences
https://www.readbyqxmd.com/read/28715335/evolutionary-model-for-the-statistical-divergence-of-paralogous-and-orthologous-gene-pairs-generated-by-whole-genome-duplication-and-speciation
#4
Yue Zhang, Chunfang Zheng, David Sankoff
We outline a principled approach to the analysis of duplicate gene similarity distributions, based on a model integrating sequence divergence and the process of fractionation of duplicate genes resulting from whole genome duplication (WGD). This model allows us predict duplicate gene similarity distributions for series of two or three WGD, for whole genome triplication followed by a WGD, and for triplication, followed by speciation, followed by WGD. We calculate the probabilities of all possible fates of a gene pair as its two members proliferate or are lost, predicting the number of surviving pairs from each event...
July 14, 2017: IEEE/ACM Transactions on Computational Biology and Bioinformatics
https://www.readbyqxmd.com/read/28714679/spectroscopic-study-of-the-reversible-chemical-reduction-and-reoxidation-of-substitutional-cr-ions-in-sr2tio4
#5
Keith A Lehuta, Anubhab Haldar, Dongming Zhou, Kevin R Kittilstved
The solid-state synthesis and controllable speciation of Cr dopants in the layered perovskite Sr2TiO4 is reported. We employed a chemical reduction procedure with NaBH4 at relatively mild temperatures (<450 °C) to impart sensitive control over the relative concentration of Cr(3+) dopants, the charge-state of oxygen-vacancy defects, and presence of Ti(3+) defects in highly reduced Cr-doped Sr2TiO4. The electron paramagnetic resonance (EPR) spectra of the reduced powder samples reveal a 12-fold increase in the Cr(3+) concentration within the axially compressed Ti(4+)-site of the Sr2TiO4 host...
July 17, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28714481/evolutionary-biology-through-the-lens-of-budding-yeast-comparative-genomics
#6
REVIEW
Souhir Marsit, Jean-Baptiste Leducq, Éléonore Durand, Axelle Marchant, Marie Filteau, Christian R Landry
The budding yeast Saccharomyces cerevisiae is a highly advanced model system for studying genetics, cell biology and systems biology. Over the past decade, the application of high-throughput sequencing technologies to this species has contributed to this yeast also becoming an important model for evolutionary genomics. Indeed, comparative genomic analyses of laboratory, wild and domesticated yeast populations are providing unprecedented detail about many of the processes that govern evolution, including long-term processes, such as reproductive isolation and speciation, and short-term processes, such as adaptation to natural and domestication-related environments...
July 17, 2017: Nature Reviews. Genetics
https://www.readbyqxmd.com/read/28713419/population-structure-genetic-diversity-and-evolutionary-history-of-kleinia-neriifolia-asteraceae-on-the-canary-islands
#7
Ye Sun, Carlos F Vargas-Mendoza
Kleinia neriifolia Haw. is an endemic species on the Canarian archipelago, this species is widespread in the coastal thicket of all the Canarian islands. In the present study, genetic diversity and population structure of K. neriifolia were investigated using chloroplast gene sequences and nuclear SSR (simple sequence repeat). The differentiation among island populations, the historical demography, and the underlying evolutionary scenarios of this species are further tested based on the genetic data. Chloroplast diversity reveals a strong genetic divergence between eastern islands (Gran Canaria, Fuerteventura, and Lanzarote) and western islands (EI Hierro, La Palma, La Gomera, Tenerife), this west-east genetic divergence may reflect a very beginning of speciation...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28712083/predictive-statistical-modelling-of-cadmium-content-in-durum-wheat-grain-based-on-soil-parameters
#8
Yoann Viala, Julien Laurette, Laurence Denaix, Emmanuelle Gourdain, Benoit Méléard, Christophe Nguyen, André Schneider, Valérie Sappin-Didier
Regulatory limits on cadmium (Cd) content in food products are tending to become stricter, especially in cereals, which are a major contributor to dietary intake of Cd by humans. This is of particular importance for durum wheat, which accumulates more Cd than bread wheat. The contamination of durum wheat grain by Cd depends not only on the genotype but also to a large extent on soil Cd availability. Assessing the phytoavailability of Cd for durum wheat is thus crucial, and appropriate methods are required. For this purpose, we propose a statistical model to predict Cd accumulation in durum wheat grain based on soil geochemical properties related to Cd availability in French agricultural soils with low Cd contents and neutral to alkaline pH (soils commonly used to grow durum wheat)...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28711674/hplc-hg-afs-determination-of-arsenic-species-in-acute-promyelocytic-leukemia-apl-plasma-and-blood-cells
#9
Meihua Guo, Wenjing Wang, Xin Hai, Jin Zhou
Arsenic trioxide (ATO) has been successfully used in the treatment of acute promyelocytic leukemia (APL). To clarify the arsenic species in APL patients, high performance liquid chromatography-hydride generation-atomic fluorescence spectrometry (HPLC-HG-AFS) and HG-AFS methods were developed and validated to quantify the plasma concentrations of inorganic arsenic (As(III) and As(V)) and methylated metabolites (MMA and DMA), and the total amounts of arsenic in blood cells and plasma. Blood cells and plasma were digested with mixtures of HNO3H2O2 and analyzed by HG-AFS...
July 6, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28710730/remediation-of-chromium-contaminated-soil-by-electrokinetics-and-electrokinetics-coupled-with-caal-ldh-permeable-reaction-barrier
#10
Yunfeng Xu, Wei Xia, Hetian Hou, Jia Zhang, Guangren Qian
The remediation of Cr(VI)-contaminated soil was investigated by electrokinetic (EK) and permeable-reactive-barrier assisted electrokinetic (EK-PRB). The medium of PRB was hydrocalumite (CaAl-LDH). The results showed that removal efficiency of hexavalent chromium (Cr(VI)) in EK-PRB and EK system was 96.49 and 85.50%, respectively. Simultaneously, the removal efficiency of total chromium (TCr) was 69.34 and 40.97% after 120-h treatment. The XRD, FTIR, and XPS analyses indicated that the reactive barrier media of CaAl-LDH successfully captured the chromium...
July 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28710457/methyl-cpg-binding-domain-proteins-inhibit-interspecies-courtship-and-promote-aggression-in-drosophila
#11
Tarun Gupta, Hannah R Morgan, Jonathan C Andrews, Edmond R Brewer, Sarah J Certel
Reproductive isolation and speciation are driven by the convergence of environmental and genetic variation. The integration of these variation sources is thought to occur through epigenetic marks including DNA methylation. Proteins containing a methyl-CpG-binding domain (MBD) bind methylated DNA and interpret epigenetic marks, providing a dynamic yet evolutionarily adapted cellular output. Here, we report the Drosophila MBD-containing proteins, dMBD-R2 and dMBD2/3, contribute to reproductive isolation and survival behavioral strategies...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710389/continuation-of-the-genetic-divergence-of-ecological-speciation-by-spatial-environmental-heterogeneity-in-island-endemic-plants
#12
Bing-Hong Huang, Chih-Wei Huang, Chia-Lung Huang, Pei-Chun Liao
Divergent selection plays a critical role not only as a speciation driver but also in maintaining post-speciation divergence. In the absence of direct evidence, ancestral interspecific gene flow between incipient species can reflect ancient selective pressure for ecological speciation. In the present study, two late-Pleistocene diverged species endemic to Taiwan, Scutellaria playfairii and S. tashiroi, were spatially and ecologically partitioned with partial overlap. Multilocus genome-scan analyses and in silico evaluation revealed ancestral interspecific gene flow but distinct genetic compositions, implying that adaptive divergence contributed to their speciation...
July 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28710265/francisella-like-endosymbionts-and-rickettsia-species-in-local-and-imported-hyalomma-ticks
#13
Tal Azagi, Eyal Klement, Gidon Perlman, Yaniv Lustig, Kosta Y Mumcuoglu, Dmitry Apanaskevich, Yuval Gottlieb
Hyalomma ticks (Acari: Ixodidae) are hosts for Francisella like endosymbionts (FLE) and may serve as vectors of zoonotic disease agents. This study was aimed to provide initial characterization of the interaction between Hyalomma and FLE and to determine the prevalence of pathogenic Rickettsia in these ticks. Hyalomma marginatum, H. rufipes, H. dromedarii, H. aegyptium and H. excavatum ticks, identified morphologically and molecularly, were collected from different hosts and locations, representing the genus distribution in Israel, as well as from migratory birds...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28709925/the-infinitesimal-model-definition-derivation-and-implications
#14
N H Barton, A M Etheridge, A Véber
Our focus here is on the infinitesimal model. In this model, one or several quantitative traits are described as the sum of a genetic and a non-genetic component, the first being distributed within families as a normal random variable centred at the average of the parental genetic components, and with a variance independent of the parental traits. Thus, the variance that segregates within families is not perturbed by selection, and can be predicted from the variance components. This does not necessarily imply that the trait distribution across the whole population should be Gaussian, and indeed selection or population structure may have a substantial effect on the overall trait distribution...
July 11, 2017: Theoretical Population Biology
https://www.readbyqxmd.com/read/28709485/biofilm-colonization-of-mycobacterium-abscessus-new-threat-in-hospital-acquired-surgical-site-infection
#15
Roumi Ghosh, Soumen Das, Harish Kela, Asmita De, Jayeeta Haldar, Prasanta Kumar Maiti
INTRODUCTION: Rapidly growing non-tuberculous mycobacteria (NTM) are hazardous cause of post-operative soft tissue infection leading to nosocomial outbreaks following various surgical procedures, especially laparoscopic surgeries using heat sensitive, non-autoclavable surgical instruments. METHODOLOGY: Surgery department of our hospital noticed increase in rate of post-laparoscopic abdominal port site infection (PSI) and informed the Microbiology Department. A prospective investigational study of defined cases with the aim of source tracing and formulation of infection control measures was initiated...
July 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28709069/characterization-of-changes-in-floc-morphology-extracellular-polymeric-substances-and-heavy-metals-speciation-of-anaerobically-digested-biosolid-under-treatment-with-a-novel-chelated-fe-2-catalyzed-fenton-process
#16
Juanjuan He, Peng Yang, Weijun Zhang, Bingdi Cao, Hua Xia, Xi Luo, Dongsheng Wang
A novel chelated-Fe(2+) catalyzed Fenton process (CCFP) was developed to enhance dewatering performance of anaerobically digested biosolid, and changes in floc morphology, extracellular polymeric substances (EPS) and heavy metals speciation were also investigated. The results showed that addition of chelating agents caused EPS solubilization by binding multivalent cations. Like traditional Fenton, CCFP performed well in improving anaerobically digested sludge dewatering property. The highly active radicals (OH, O2(-)) produced in classical Fenton and CCFP were responsible for sludge flocs destruction and consequently degradation of biopolymers into small molecules...
July 3, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28704663/effect-of-sulfide-on-as-iii-and-as-v-sequestration-by-ferrihydrite
#17
Zhixi Zhao, Shaofeng Wang, Yongfeng Jia
The sulfide-induced change in arsenic speciation is often coupled to iron geochemical processes, including redox reaction, adsorption/desorption and precipitation/dissolution. Knowledge about how sulfide influenced the coupled geochemistry of iron and arsenic was not explored well up to now. In this work, retention and mobilization of As(III) and As(V) on ferrihydrite in sulfide-rich environment was studied. The initial oxidation states of arsenic and the contact order of sulfide notably influenced arsenic sequestration on ferrihydrite...
June 30, 2017: Chemosphere
https://www.readbyqxmd.com/read/28702905/assessment-of-biofilm-changes-and-concentration-depth-profiles-during-arsenopyrite-oxidation-by-acidithiobacillus-thiooxidans
#18
Hugo Ramírez-Aldaba, Jorge Vazquez-Arenas, Fabiola S Sosa-Rodríguez, Donato Valdez-Pérez, Estela Ruiz-Baca, Jessica Viridiana García-Meza, Gabriel Trejo-Córdova, René H Lara
Biofilm formation and evolution are key factors to consider to better understand the kinetics of arsenopyrite biooxidation. Chemical and surface analyses were carried out using Raman spectroscopy, scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), glow discharge spectroscopy (GDS), and protein analysis (i.e., quantification) in order to evaluate the formation of intermediate secondary compounds and any significant changes arising in the biofilm structure of Acidithiobacillus thiooxidans during a 120-h period of biooxidation...
July 12, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28702705/wolbachia-in-the-genus-bicyclus-a-forgotten-player
#19
Anne Duplouy, Oskar Brattström
Bicyclus butterflies are key species for studies of wing pattern development, phenotypic plasticity, speciation and the genetics of Lepidoptera. One of the key endosymbionts in butterflies, the alpha-Proteobacterium Wolbachia pipientis, is affecting many of these biological processes; however, Bicyclus butterflies have not been investigated systematically as hosts to Wolbachia. In this study, we screen for Wolbachia infection in several Bicyclus species from natural populations across Africa as well as two laboratory populations...
July 12, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28702491/data-on-the-time-of-integration-of-the-human-mitochondrial-pseudogenes-numts-into-the-nuclear-genome
#20
Konstantin Gunbin, Leonid Peshkin, Konstantin Popadin, Sofia Annis, Rebecca R Ackermann, Konstantin Khrapko
The data and methods presented in this article are supplementing the research article "Integration of mtDNA pseudogenes into the nuclear genome coincides with speciation of the human genus. A hypothesis", DOI: 10.1016/j.mito.2016.12.001 (Gunbin et al., 2017) [1]. Mitochondrial DNA is known to get inserted into nuclear DNA to form NUMTs, i.e. nuclear DNA pseudogenes of the mtDNA. We present here the sequences of selected NUMTs, in which time of integration can be determined with sufficient precision. We report their chromosomal positions , their position within the great ape mtDNA phylogeny, and their times of integration into the nuclear genome...
August 2017: Data in Brief
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