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Embryo transfer. Abortion

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https://www.readbyqxmd.com/read/27821126/efficient-generation-of-ggta1-null-diannan-miniature-pigs-using-talens-combined-with-somatic-cell-nuclear-transfer
#1
Wenmin Cheng, Heng Zhao, Honghao Yu, Jige Xin, Jia Wang, Luyao Zeng, Zaimei Yuan, Yubo Qing, Honghui Li, Baoyu Jia, Cejun Yang, Youfeng Shen, Lu Zhao, Weirong Pan, Hong-Ye Zhao, Wei Wang, Hong-Jiang Wei
BACKGROUND: α1,3-Galactosyltransferase (GGTA1) is essential for the biosynthesis of glycoproteins and therefore a simple and effective target for disrupting the expression of galactose α-1,3-galactose epitopes, which mediate hyperacute rejection (HAR) in xenotransplantation. Miniature pigs are considered to have the greatest potential as xenotransplantation donors. A GGTA1-knockout (GTKO) miniature pig might mitigate or prevent HAR in xenotransplantation. METHODS: Transcription activator-like effector nucleases (TALENs) were designed to target exon 6 of porcine GGTA1 gene...
November 8, 2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27803585/comparative-analysis-of-perinatal-outcome-of-spontaneous-pregnancy-reduction-and-multifetal-pregnancy-reduction-in-triplet-pregnancies-conceived-after-assisted-reproductive-technique
#2
Shilpa Bhandari, Ishita Ganguly, Pallavi Agrawal, Shweta Bhandari, Aparna Singh, Nitika Gupta
INTRODUCTION: With the advent of assisted reproductive treatment options, the incidence of multiple pregnancies has increased. Although the need for elective single embryo transfer is emphasized time and again, its uniform applicability in practice is yet a distant goal. In view of the fact that triplet and higher order pregnancies are associated with significant fetomaternal complications, the fetal reduction is a commonly used option in such cases. This retrospective study aims to compare the perinatal outcome in patients with triplet gestation who have undergone spontaneous fetal reduction (SFR) as against those in whom multifetal pregnancy reduction (MFPR) was done...
July 2016: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/27762465/ultrasound-sclerotherapy-pretreatment-could-obtain-a-similar-effect-to-surgical-intervention-on-improving-the-outcomes-of-in-vitro-fertilization-for-patients-with-hydrosalpinx
#3
Xiao-Min Song, Hong Jiang, Wen-Xiang Zhang, Yun Zhou, Feng Ni, Xue-Mei Wang
AIM: The aim of this study was to compare the clinical outcomes of in vitro fertilization (IVF) for patients with hydrosalpinx between ultrasound sclerotherapy and surgical intervention. METHODS: The data of 482 IVF/intracytoplasmic sperm injection (ICSI) cycles were divided into three groups according to different intervention protocols before IVF/ICSI. Group A included 265 cycles in which ultrasound sclerotherapy pretreatment was carried out before controlled ovarian hyperstimulation...
October 20, 2016: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/27752891/patients-with-cervical-ureaplasma-urealyticum-and-chlamydia-trachomatis-infection-undergoing-ivf-icsi-et-the-need-for-new-paradigm
#4
Qing-Zhen Xie, Wang-Ming Xu, Qian-Rong Qi, Zeng-Li Luo, Lan Dong
Genital tract infections with ureaplasma urealyticum (UU) and chlamydia trachomatis (CT) are the most frequent sexually-transmitted disease worldwide. UU and CT infections are considered to be the leading cause for infertility and adverse pregnancy outcomes. However, little is known about the specific effect of cervical UU and CT infections on the etiology of female infertility, as well as the pregnancy outcomes of the patients undergoing in vitro fertilization/intracytoplasmic sperm injection-embryo transfer (IVF/ICSI-ET)...
October 2016: Journal of Huazhong University of Science and Technology. Medical Sciences
https://www.readbyqxmd.com/read/27752285/molecular-cytogenetic-analysis-of-early-spontaneous-abortions-conceived-from-varying-assisted-reproductive-technology-procedures
#5
Tonghua Wu, Biao Yin, Yuanchang Zhu, Guangui Li, Lijun Ye, Chunmei Chen, Yong Zeng, Desheng Liang
BACKGROUND: Spontaneous abortion (SA) is the most common complication of pregnancy, and chromosome aberrations are the principal cause of the first trimester abortuses in natural conception (NC) The increasing use of assisted reproductive technology (ART) has raised concern about chromosome abnormalities in ART-initiated pregnancies. Up to date, the literature on the risk of aneuploidy in failed pregnancies among various ART factors remain limited and inconclusive. This study aimed to explore the genetic etiology of pregnancy loss conceived from varying ART procedures...
2016: Molecular Cytogenetics
https://www.readbyqxmd.com/read/27752179/-effect-of-catheter-choice-during-embryo-transfer-on-the-clinical-outcome-of-in-vitro-fertilization-embryo-transfer
#6
X L Ren, P Liu, Y Lian, J Huang, X Y Zheng, Y J Zhu, J Qiao
OBJECTIVE: To compare two flexible embryo catheters and determine whether clinical outcome differs in the in vitro fertilization-embryo transfer (IVF-ET) cycles. METHODS: This prospective control study was conducted by one doctor between July 2012 and November 2013. In the study, 2 064 patients undergoing fresh embryo transfer by using IVF-ET/intracytoplasmic sperm injection (ICSI)-ET in Reproductive Medical Center of Peking University Third Hospital were recruited...
October 18, 2016: Beijing da Xue Xue Bao. Yi Xue Ban, Journal of Peking University. Health Sciences
https://www.readbyqxmd.com/read/27741507/effect-of-parental-physiological-conditions-and-assisted-reproductive-technologies-on-the-pregnancy-and-birth-outcomes-in-infertile-patients
#7
Xinqi Zhong, Jianqiao Liu, Qiliang Cui, Shaozhen Liang, Yuanqing Lin, Haiying Liu, Qiyi Zeng
Assisted reproductive technologies (ART) are widely used to treat infertility. Emerging evidence suggested that ART was associated with perinatal or neonatal problems, however, little is known about the ART related risk factors. Here using 21136 ART cases, we determined the impacts of parental physiological conditions in the ART mediated pregnancy outcomes. In addition, we further evaluated the effects of three different ART methods (frozen-thawed embryo transfer [FET], in vitro fertilization [IVF] and intracytoplasmic sperm injection [ICSI]) in the pregnancy and birth outcomes in ART mediated pregnancy...
October 10, 2016: Oncotarget
https://www.readbyqxmd.com/read/27738657/gnrh-agonist-trigger-versus-hcg-trigger-in-gnrh-antagonist-in-ivf-icsi-cycles-a-review-article
#8
Ashraf Alyasin, Shayesteh Mehdinejadiani, Marzieh Ghasemi
Routinely, a bolus of 5.000-10.000 IU human chorionic gonadotropin (hCG) is used for the final follicular maturation and ovulation as a standard method. HCG has the same effect of luteinizing hormone (LH) with long half-life. It has the long lutheotrophic effect which increases the risk of ovarian hyper stimulation syndrome (OHSS). Recently, gonadotropin-releasing hormone agonist (GnRH-a) trigger has been used for the induction of final follicular maturation and ovulation with the aim of reducing the OHSS risk...
September 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27680029/intrauterine-administration-of-hcg-immediately-after-oocyte-retrieval-and-the-outcome-of-icsi-a-randomized-controlled-trial
#9
N Navali, A Gassemzadeh, L Farzadi, S Abdollahi, M Nouri, K Hamdi, F Mallah, F Jalilvand
STUDY QUESTION: Does the intrauterine administration of hCG immediately after oocyte retrieval in antagonist cycles with ICSI and fresh embryo transfer (ET) influence the implantation rate or chemical and clinical pregnancy rates? SUMMARY ANSWER: The intrauterine administration of hCG after oocyte retrieval increases the implantation rate and chemical and clinical pregnancy rates. WHAT IS KNOWN ALREADY: Over half of IVF/ICSI cycles fail due to implantation failure...
November 2016: Human Reproduction
https://www.readbyqxmd.com/read/27636504/pregnancy-delivery-and-neonatal-outcomes-of-in-vitro-fertilization-embryo-transfer-in-patient-with-previous-cesarean-scar
#10
Ningyuan Zhang, Hua Chen, Zhipeng Xu, Bin Wang, Haixiang Sun, Yali Hu
BACKGROUND What role should previous cesarean section play in affecting clinical pregnancy outcomes and avoiding the complications of in vitro fertilization? In this article, we focus on elective single-embryo transfer (eSET) versus double-embryo transfer (DET) and assess the clinical efficacy and safety of eSET in patients who have a previous cesarean scar. MATERIAL AND METHODS The pregnancy, delivery, and neonatal outcomes of 130 patients who had a previous cesarean scar and received in vitro fertilization-embryo transfer (IVF-ET) were retrospectively analyzed...
2016: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/27614789/intralipid-supplementation-in-women-with-recurrent-spontaneous-abortion-and-elevated-levels-of-natural-killer-cells
#11
Dina M R Dakhly, Yomna A Bayoumi, Mohamed Sharkawy, Sherine H Gad Allah, Mohamed A Hassan, Hisham M Gouda, Ahmed T Hashem, Dina L Hatem, Mona F Ahmed, Waleed El-Khayat
OBJECTIVE: To investigate the efficacy of intralipid supplementation in women with recurrent spontaneous abortion (RSA) and elevated natural killer cell activity undergoing in vitro fertilization/intracytoplasmic sperm injection. METHODS: Between February 10, 2013, and April 30, 2015, a double-blind randomized controlled study was conducted at a center in Egypt. Women with unexplained secondary infertility, RSA, and elevated levels of natural killer cells (>12%) were enrolled and randomly assigned to receive intralipid (2mL diluted at 20% in 250mL saline) or saline (250mL) infusion on the day of oocyte retrieval using random numbers and sealed envelopes...
December 2016: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/27614356/correlation-between-chromosomal-distribution-and-embryonic-findings-on-ultrasound-in-early-pregnancy-loss-after-ivf-embryo-transfer
#12
Yan Ouyang, Yueqiu Tan, Yan Yi, Fei Gong, Ge Lin, Xihong Li, Guangxiu Lu
STUDY QUESTION: Do early pregnancy losses (EPLs) with and without embryos differ in chromosomal distributions? SUMMARY ANSWER: The chromosomal abnormality rate is significantly higher in miscarriages with embryos than without after in vitro fertilization (IVF)-embryo transfer. WHAT IS KNOWN ALREADY: Chromosomal abnormalities are the main causes of EPLs, the rate of which is up to 24-30% in the IVF population. Little research has been conducted on the correlations between the chromosomal distributions of EPL and the existence of an embryo or with the postmortem embryonic pole length, and the existing results have been inconsistent...
October 2016: Human Reproduction
https://www.readbyqxmd.com/read/27600956/45-x-product-of-conception-after-preimplantation-genetic-diagnosis-and-euploid-embryo-transfer-evidence-of-a-spontaneous-conception-confirmed-by-dna-fingerprinting
#13
Daniela Bettio, Antonio Capalbo, Elena Albani, Laura Rienzi, Valentina Achille, Anna Venci, Filippo Maria Ubaldi, Paolo Emanuele Levi Setti
BACKGROUND: Preimplantation genetic screening (PGS) provides an opportunity to eliminate a potential implantation failure due to aneuploidy in infertile couples. Some studies clearly show that twins following single embryo transfer (SET) can be the result of a concurrent natural conception and an incidence as high as 1 in 5 twins has been reported. In our case PGS was performed on trophectoderm (TE) biopsies by quantitative polymerase chain reaction (qPCR). The product of conception (POC) was cytogenetically investigated after selection of the placental villi by means of the direct method...
2016: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/27561938/-application-of-array-based-comparative-genomic-hybridization-technique-in-genetic-analysis-of-patients-with-spontaneous-abortion
#14
Y Chu, D Wu, Q F Hou, X D Huo, Y Gao, T Wang, H D Wang, Y L Yang, S X Liao
OBJECTIVE: To investigate the value of array-based comparative genomic hybridization (array-CGH) technique for the detection of chromosomal analysis of miscarried embryo, and to provide genetic counseling for couples with spontaneous abortion. METHODS: Totally 382 patients who underwent miscarriage were enrolled in this study. All aborted tissues were analyzed with conventional cytogenetic karyotyping and array-CGH, respectively. RESULTS: Through genetic analysis, all of the 382 specimens were successfully analyzed by array-CGH (100...
August 25, 2016: Zhonghua Fu Chan Ke za Zhi
https://www.readbyqxmd.com/read/27525331/modified-natural-cycle-frozen-thawed-embryo-transfer-in-patients-with-repeated-implantation-failure-an-observational-study
#15
Soheila Arefi, Ahmad Hoseini, Fattaneh Farifteh, Hojjat Zeraati
BACKGROUND: Natural endometrium in Frozen-thawed Embryo Transfer (FET) may have some benefits upon implantation in patients with Repeated Implantation Failure (RIF). It might be due to possible differences between natural and stimulated endometrial growth factors and cytokins secretions. OBJECTIVE: The objective of this study was to compare the pregnancy rate of FET on modified natural cycle versus hormone replacement therapy (HRT) cycle endometrium in patients with RIF...
July 2016: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/27470423/comparison-of-ultrasound-guided-endometrial-polypectomy-carried-out-on-the-oocyte-retrieval-day-and-the-first-day-of-ovarian-stimulation-in-ivf-icsi-cycles
#16
Jei-Won Moon, Chung-Hoon Kim, So-Yun Park, Sung-Hoon Kim, Hee-Dong Chae, Byung-Moon Kang
In this retrospective cohort study, the effect of endometrial polypectomy carried out on the day of oocyte retrieval and on the first day of ovarian stimulation in patients with a large (≥10 mm) endometrial polyp undergoing IVF and intractyoplasmic sperm injection (ICSI) was investigated and compared. A total of 74 eligible IVF-ICSI cycles in 74 women who underwent endometrial polypectomy on either the day of oocyte pick-up (late polypectomy group, 39 cycles) or the first day of ovarian stimulation (early polypectomy group, 35 cycles) between January 2007 and July 2012 were included in this study...
September 2016: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/27449969/a-study-of-intrauterine-infusion-of-human-chorionic-gonadotropin-hcg-before-frozen-thawed-embryo-transfer-after-two-or-more-implantation-failures
#17
Pinxiu Huang, Lihong Wei, Xinlin Li
OBJECTIVE: To investigate the effect of intrauterine infusion of human chorionic gonadotropin (hCG) before frozen-thawed embryo transfer (FET) after two or more implantation failures (TIFs). METHODS: The study was a prospective randomized single-blind study of 161 cycles in patients undergoing FET who had TIFs. The intervention group received an intrauterine injection of 1000 IU of hCG before embryo transfer (ET) (n = 62). A placebo group (n = 49) received an intrauterine injection of physiological saline before ET...
July 22, 2016: Gynecological Endocrinology
https://www.readbyqxmd.com/read/27408716/efficient-production-of-pronuclear-embryos-in-breeding-and-nonbreeding-season-for-generating-transgenic-sheep-overexpressing-tlr4
#18
Yan Li, Di Lian, Shoulong Deng, Xiaosheng Zhang, Jinlong Zhang, Wenting Li, Hai Bai, Zhixian Wang, Hongping Wu, Juncai Fu, Hongbing Han, Jianzhong Feng, Guoshi Liu, Ling Lian, Zhengxing Lian
BACKGROUND: Brucella is a zoonotic Gram-negative pathogen that causes abortion and infertility in ruminants and humans. TLR4 is the receptor for LPS which can recognize Brucella and initiate antigen-presenting cell activities that affect both innate and adaptive immunity. Consequently, transgenic sheep over-expressing TLR4 are an suitable model to investigate the effects of TLR4 on preventing Brucellosis. In this study, we generated transgenic sheep overexpressing TLR4 and aimed to evaluate the effects of different seasons (breeding and non-breeding season) on superovulation and the imported exogenous gene on growth...
2016: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/27400398/a-modified-natural-cycle-results-in-higher-live-birth-rate-in-vitrified-thawed-embryo-transfer-for-women-with-regular-menstruation
#19
Yichun Guan, Hongfang Fan, Aaron K Styer, Zhiying Xiao, Zhen Li, Jianrui Zhang, Lijun Sun, Xingling Wang, Zhan Zhang
There is no consensus regimen for the optimal endometrial preparation for cryopreservation and vitrified-thawed embryo transfer cycles. This is largely caused by the lack of sufficient investigation and analyses on the respective pregnancy and perinatal outcomes by different regimens. This study aimed to compare both pregnancy and perinatal outcomes between the modified natural and artificial cycles in vitrified-thawed day three embryo transfer for women with regular menstruation. A total of 1,482 vitrified-thawed day three embryo transfer cycles were reviewed including 427 modified natural cycles (NC), 132 ovulation induction cycles (OC), 794 artificial cycles (AC), and 129 GnRH agonist artificial cycles (GAC)...
October 2016: Systems Biology in Reproductive Medicine
https://www.readbyqxmd.com/read/27366008/the-risk-factors-and-pregnancy-outcomes-of-48-cases-of-heterotopic-pregnancy-from-a-single-center
#20
Ji Hyun Jeon, Yu Im Hwang, Im Hee Shin, Chan Woo Park, Kwang Moon Yang, Hye Ok Kim
The purpose of this study was to investigate risk factors that are associated with heterotopic pregnancy (HP) following in vitro fertilization (IVF)-embryo transfer (ET) and to demonstrate the outcomes of HP after the surgical treatment of ectopic pregnancies. Forty-eight patients from a single center, who were diagnosed with HP between 1998 and 2012 were included. All of the patients had received infertility treatments, such as Clomid with timed coitus (n = 1, 2.1%), superovulation with intrauterine insemination (n = 7, 14...
July 2016: Journal of Korean Medical Science
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