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Paride Mantecca, Elisa Moschini, Patrizia Bonfanti, Umberto Fascio, Ilana Perelshtein, Anat Lipovsky, Giuseppe Chirico, Renato Bacchetta, Luca Del Giacco, Anita Colombo, Aharon Gedanken
The increased resistances to conventional antibiotics determine a strong need for new antibacterials, and specific syntheses at the nanoscale promise to be helpful in this field. A novel Zinc-doped Copper oxide nanocomposite (nZn-CuO) has been recently sonochemically synthesized and successfully tested also against multi-drug resistant bacteria. After synthesis and characterization of the physicochemical properties, the new nZn-CuO is here evaluated by the Frog Embyo Teratogenesis Assay-Xenopus test for its toxicological potential and this compared with that of nCuO and nZnO synthesized under the same conditions...
July 2015: Toxicological Sciences: An Official Journal of the Society of Toxicology
Nicole Rebscher
Elucidating the origin of germ cells in embryos and larvae is often obscured by the fact that the typical germ cell markers vasa, nanos and piwi are not exclusively expressed in primordial germ cells (PGCs), but are also commonly found in undifferentiated somatic tissues and stem cells as part of an evolutionary conserved 'germline multipotency program' (Juliano et al., 2010). Hidden in the crowd of undifferentiated cells, the PGCs have occasionally been overlooked and their formation during early embryogenesis was only revealed recently by new methodological approaches (e...
2014: International Journal of Developmental Biology
Jannathan Mamut, Dun Yan Tan, Carol C Baskin, Jerry M Baskin
BACKGROUND AND AIMS: Little is known about morphological (MD) or morphophysiological (MPD) dormancy in cold desert species and in particular those in Liliaceae sensu lato, an important floristic element in the cold deserts of Central Asia with underdeveloped embyos. The primary aim of this study was to determine if seeds of the cold desert liliaceous perennial ephemeral Eremurus anisopterus has MD or MPD, and, if it is MPD, then at what level. METHODS: Embryo growth and germination was monitored in seeds subjected to natural and simulated natural temperature regimes and the effects of after-ripening and GA3 on dormancy break were tested...
October 2014: Annals of Botany
Chung-Hoon Kim, You-Jeong Lee, Kyung-Hee Lee, Su-Kyung Kwon, Sung-Hoon Kim, Hee-Dong Chae, Byung-Moon Kang
OBJECTIVE: To evaluate the effect of progesterone supplementation during the luteal phase on pregnancy outcome in natural frozen-thawed embyo transfer (FTET) cycles. METHODS: In this retrospective cohort study, 228 consecutive patients who underwent FTET cycles between January 2009 and September 2012 were included. One hundred forty-five patients received luteal progesterone support (P group) but 83 patients did not receive any progesterone supplementation during luteal phase (control group)...
July 2014: Obstetrics & Gynecology Science
Teruhiro Okuyama, Yasuko Isoe, Masahito Hoki, Yuji Suehiro, Genki Yamagishi, Kiyoshi Naruse, Masato Kinoshita, Yasuhiro Kamei, Atushi Shimizu, Takeo Kubo, Hideaki Takeuchi
BACKGROUND: Genetic mosaic techniques have been used to visualize and/or genetically modify a neuronal subpopulation within complex neural circuits in various animals. Neural populations available for mosaic analysis, however, are limited in the vertebrate brain. METHODOLOGY/PRINCIPAL FINDINGS: To establish methodology to genetically manipulate neural circuits in medaka, we first created two transgenic (Tg) medaka lines, Tg (HSP:Cre) and Tg (HuC:loxP-DsRed-loxP-GFP)...
2013: PloS One
P Rizzo, E Raffone, V Benedetto
OBJECTIVE: The aim of the study was to evaluate the efficacy of a treatment with myo-inositol plus folic acid plus melatonin compared with myo-inositol plus folic acid alone on oocyte quality in women underwent in vitro fertilization (IVF) cycles. DESIGN: A prospective, clinical trial. MATERIALS AND METHODS: Starting on the day of GnRH administration, 65 women undergoing IVF cycles were randomized in two groups to receive myo-inositol plus folic acid plus melatonin (32 women, group A), and myo-inositol plus folic acid (33 women, group B), administered continuously...
June 2010: European Review for Medical and Pharmacological Sciences
H Matsumoto, K Toyofuku, I Taniguchi, M Haraokaz, T Utsunomiya, N Takai
Among young women, the incidence of uterine corpus cancer is increasing. Most young women can not preserve fertility because simple total hysterectomy with bilateral salpingo-oophorectomy is the standard method for early endometrial cancer so far. We present a case of early endometrial adenocarcinoma which succeeded in pregnancy and delivery after resectoscopic surgery. Following a circumferential resection of the lesion including the mucosa and muscle layer under resectoscopic guidance, the patient became pregnant by means of in vitro fertilization-embyo transfer with hormone replenishment...
2009: European Journal of Gynaecological Oncology
Yi-Rang Na, Seung-Hyeok Seok, Dong-Jae Kim, Ju-Hee Han, Tae-Hyun Kim, Hyoun Jung, Jae-Hak Park
Sphere-forming abilities in culture condition are considered a hallmark of cancer stem-like cells, which represents tumor cell invasiveness and stem-like characteristics. We aimed to show that the sphere-forming subpopulation of human malignant melanoma cell line WM-266-4 acts differently to zebrafish embryo extracts compared with their bulk counterpart. Spheres were maintained in neural stem cell culture conditions. The embryos of zebrafish at specific developmental stages were collected and the extracts were purified under 100 kDa...
August 2009: Cancer Science
Gun Hyuk Jang, In-Sook Park, Sun Hee Lee, Tae-Lin Huh, You Mie Lee
Malachite green (MG) is a triphenyl methane dye used in various fields that demonstrates high toxicity to bacteria and mammalian cells. When bud stage zebrafish embryos were treated with MG at 125, 150, and 175ppb for 14h, the development of trunk including intersomitic vessels was inhibited in MG-treated flk-1-GFP transgenic embyos. MG clearly induced whole growth retardation. MG induced severe cell death in trunk intersomite region of zebrafish embryos and in human vascular endothelial cells in a dose-dependent manner...
May 8, 2009: Biochemical and Biophysical Research Communications
Yi-Rang Na, Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Sung-Hoon Park, Hyun-Kyoung Lee, Jae-Hak Park
3,3',4,4',5-Pentachlorinated biphenyls 126 (PCB126) is a global environmental contaminant that can induce cellular oxidative stress. We investigated whether vitamin E can protect against toxicity from PCB126 during zebrafish (Danio rerio) development. Zebrafish embryos were exposed to 100nM PCB126 and compared with a second group that was co-exposed with 100muM vitamin E until 5 days post fertilization. PCB126 induced pericardial sac edema, yolk sac edema, and growth retardation in zebrafish embyos. In contrast, vitamin E co-exposure group did not show any gross changes...
March 2009: Ecotoxicology and Environmental Safety
Michiaki Yamashita, Nanami Mizusawa, Misako Hojo, Takeshi Yabu
The pro-apoptotic caspase-3 gene has been shown to have key functions in the execution of apoptosis (programmed cell death) in vertebrate cells. However, the central role of caspase-3 in morphogenesis during development remains unclear. In this study, transgenic zebrafish that overexpress full-length pro-caspase-3 were generated to determine the effects of caspase genes on vertebrate morphogenesis and stress tolerance. The enhanced expression of the full-length pro-caspase-3 cDNA induced extremely high levels of caspase activity and extensive apoptosis in the transgenic embryos, and 33-46% of F2 embyos in the transgenic lines exhibited some form of morphological abnormality...
June 2008: Journal of Experimental Biology
Harshan Pisharath
1Escherichia coli gene nfsB encodes a nitroreductase (NTR) enzyme that converts prodrugs like metronidazole (Met) and CB 1954 to cytotoxic metabolites. My coworkers and I have validated this prodrug-enzyme system in zebrafish (Danio rerio) by ubiquitously expressing NTR-EGFP fusion protein and exposing these embyos to CB 1954 and Met. These embryos showed extensive gross pathologic changes and death by 24 h of incubation in the prodrugs. They also exhibited widespread and marked apoptotic changes by 8 h of incubation in Met...
June 2007: Comparative Medicine
D Rath, H Niemann, T Tao
The objective of the present study was to test the ability of porcine follicular fluid (pFF) to improve maturation of porcine cumulus-oocyte-complexes (COC) in vitro and to observe subsequent effects on fertilization and development to late morula/blastocyst stages under in vitro conditions. The COC were incubated in Tissue Culture Medium (TCM) 199, supplemented with 1% fetal calf serum (FCS), 10% pFF collected from immature follicles (2 to 5 mm), with or without addition of 1microg/ml FSH. Control groups were matured in TCM 199 with or without FSH...
September 1995: Theriogenology
W A King, B R Yadav, K P Xu, L Picard, M A Sirard, A Verini Supplizi, K J Betteridge
The male:female ratio of developing bovine embryos produced and allowed to develop in vitro and in vivo was determined retrospectively from the cytogenetic analysis of 804 embyos. The overall male:female ratio of the 307 (38%) embryos that could be sexed was 162 (52.8%):145 (47.2%) and did not differ (P>0.05) from the expected 1:1 ratio. Among premorula stage embryos produced in vivo (n = 66) and in vitro (n = 30), the ratios were 1.2:1 and 0.76:1, respectively. Among morulae and blastocysts produced in vivo (n = 74), produced and cultured in vitro (n = 106, and produced in vitro and cultured in vivo (n = 31), the ratios were 1...
November 1991: Theriogenology
Gregory J Bootsma, G Wayne Brodland
Although three-dimensional (3-D) reconstructions of the surfaces of live embyos are vital to understanding embryo development, morphogenetic tissue movements and other factors have prevented the automation of this task. Here, we report an integrated set of software algorithms that overcome these challenges, making it possible to completely automate the reconstruction of embryo surfaces and other textured surfaces from multiview images. The process involves: 1) building accurate point correspondences using a robust deformable template block matching algorithm; 2) removing outliers using fundamental matrix calculations in conjunction with a RANSAC algorithm; 3) generating 3-D point clouds using a bundle adjustment algorithm that includes camera position and distortion corrections; 4) meshing the point clouds into triangulated surfaces using a Tight Cocone algorithm that produces water tight models; 5) refining surfaces using midpoint insertion and Laplacian smoothing algorithms; and 6) repeating these steps until a measure of convergence G, the rms difference between successive reconstructions, is below a specified threshold...
August 2005: IEEE Transactions on Bio-medical Engineering
S Kahraman, N Findikli, H Berkil, E Bakircioglu, E Donmez, S Sertyel, A Biricik
With the application of preimplantation genetic diagnosis (PGD), a possible genetic contribution of spermatozoa obtained from 47,XXY non-mosaic Klinefelter patients on preimplantation embryos was analysed in eight couples. Interpretable fluorescence in-situ hybridization results were obtained for 28 out of 33 embryos biopsied (84.8%) and 23 blastomeres were analysed for chromosomes 13, 18, 21, X and Y. Nine out of 23 embryos were diagnosed as abnormal (39.1%). Five out of nine contained sex chromosome abnormalities (55...
October 2003: Reproductive Biomedicine Online
Jouji Kokuzawa, Shinichi Yoshimura, Hideomi Kitajima, Jun Shinoda, Yasuhiko Kaku, Toru Iwama, Ryuichi Morishita, Takuya Shimazaki, Hideyuki Okano, Takahiro Kunisada, Noboru Sakai
Hepatocyte growth factor (HGF), originally cloned as a hepatocyte mitogen, has recently been reported to exhibit neurotrophic activity in addition to being expressed in different parts of the nervous system. At present, the effects of HGF on neural stem cells (NSCs) are not known. In this study, we first report the promoting effect of HGF on the proliferation of neurospheres and neuronal differentiation of NSCs. Medium containing only HGF was capable of inducing neurosphere formation. Addition of HGF to medium containing fibroblast growth factor 2 or epidermal growth factor increased both the size and number of newly formed neurospheres...
September 2003: Molecular and Cellular Neurosciences
No abstract text is available yet for this article.
May 1964: Journal de Physiologie
T Mizuno, M Saito
We have studied the prostatic bud induction in the urogenital sinus of androgen-receptor deficient mice, and found that epidermal growth factor (EGF), keratinocyte growth factor (KGF) as well as transforming growth factor-alpha (TGF-alpha) can provoke prostatic bud formation in the androgen-receptor deficient sinus cultured in the medium deprived of androgens.
1996: Comptes Rendus des Séances de la Société de Biologie et de Ses Filiales
J P Yang, W Chiang, J L Gale, N S Chen
Oral type 1 and genital type 2 Herpesvirus hominis (HVH) strains demonstrate distinctive biological properties in primary chick embryo cells (PCE) cultivated in microtest plates. With this procedure four reference strains of known types and 106 clinical isolates were differentiated as type 1 or 2. The type 1 strains showed low efficiency of infection and either no cytopathic effect (CPE) or only an incomplete CPE characterized by uniform thinning of the cell sheet in test wells. Type 2 strains had a high efficiency of infection and with CPE characterized by patchy plaque-like lesions readily distinguished from CPE of type 1 strains...
February 1975: Proceedings of the Society for Experimental Biology and Medicine
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