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calcium lactation

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https://www.readbyqxmd.com/read/28904232/effects-of-high-versus-moderate-intensity-training-on-neuroplasticity-and-functional-recovery-after-focal-ischemia
#1
Caroline Pin-Barre, Annabelle Constans, Jeanick Brisswalter, Christophe Pellegrino, Jérôme Laurin
BACKGROUND AND PURPOSE: This study was designed to compare the effects of high-intensity interval training (HIT) and moderate-intensity aerobic training (MOD) on functional recovery and cerebral plasticity during the first 2 weeks after cerebral ischemia. METHODS: Rats were randomized as follows: control (n=15), SHAM (n=9), middle cerebral artery occlusion (n=13), middle cerebral artery occlusion at day 1 (n=7), MOD (n=13), and HIT (n=13). Incremental tests were performed at day 1 (D1) and 14 (D14) to identify the running speed associated with the lactate threshold (SLT) and the maximal speed (Smax)...
September 13, 2017: Stroke; a Journal of Cerebral Circulation
https://www.readbyqxmd.com/read/28893334/metabolic-and-stress-responses-in-dairy-cows-fed-a-concentrate-rich-diet-and-submitted-to-intramammary-lipopolysaccharide-challenge
#2
S Aditya, E Humer, P Pourazad, R Khiaosa-Ard, Q Zebeli
Feeding dairy cows diets rich in grain often leads to subacute rumen acidosis (SARA), which might affect their responsiveness to immunogenic stimuli such as exogenous lipopolysaccharide (LPS), and can lead to metabolic alterations. The main objective of this study was to investigate if SARA affects the stress and metabolic health responses resulting from an intramammary LPS challenge. Before the intramammary LPS challenge, the SARA cows showed higher blood glucose and a tendency for higher lactate and aspartate aminotransferase as well as a trend toward lower β-hydroxybutyrate (BHBA) and γ-glutamyltransferase compared with control cows...
September 12, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28893316/intraosseous-blood-samples-for-point-of-care-analysis-agreement-between-intraosseous-and-arterial-analyses
#3
Milla Jousi, Simo Saikko, Jouni Nurmi
BACKGROUND: Point-of-care (POC) testing is highly useful when treating critically ill patients. In case of difficult vascular access, the intraosseous (IO) route is commonly used, and blood is aspirated to confirm the correct position of the IO-needle. Thus, IO blood samples could be easily accessed for POC analyses in emergency situations. The aim of this study was to determine whether IO values agree sufficiently with arterial values to be used for clinical decision making. METHODS: Two samples of IO blood were drawn from 31 healthy volunteers and compared with arterial samples...
September 11, 2017: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/28892414/efficacy-of-recombinant-adenovirus-expressing-a-fusion-gene-from-gm-csf-and-epstein-barr-virus-lmp2a-in-a-mouse-tumor-model
#4
Qingjie Xue, Xiuzheng Li, Chunqing Yang, Bingyuan Ji, Yunqing Li, Yingchun Yan, Xinxin Yang, Chunmei Wang, Ting Chen
In this study, purified GM-CSF and LMP2A mRNAs were amplified by PCR. Then, the GM-CSF and LMP2A sequences were connected by the polypeptide linker (Gly4Ser)3 using gene splicing by overlap extension. The constructed fusion gene GC2A was inserted into the adenovirus vector. Then the recombinant vector was introduced into HEK 293T cells by calcium phosphate transfection to package the adenovirus. The levels of antibodies against the GM-CSF and LMP2Afusion proteins were measured by ELISA, and the CTL activity of the mouse splenic lymphocytes was determined by lactate dehydrogenase (LDH) release assay...
September 11, 2017: Human Vaccines & Immunotherapeutics
https://www.readbyqxmd.com/read/28880045/-factors-influencing-first-colostrum-calcium-concentration-in-holstein-friesian-cows-and-the-relationship-to-postpartum-calcium-concentration
#5
Maria Karl, Rudolf Staufenbiel
OBJECTIVE: To investigate the factors that affect the calcium content of the first colostrum (milk exclusively of the first withdrawal) and the relationship to the blood calcium concentration. We tested the hypotheses that the lactation number is the main influence factor and that there is a relationship to the postpartum serum calcium concentration. MATERIAL AND METHODS: An observational study was conducted from April 2012 to March 2013 at a commercial dairy farm (2278 Holstein-Friesian cows)...
September 5, 2017: Tierärztliche Praxis. Ausgabe G, Grosstiere/Nutztiere
https://www.readbyqxmd.com/read/28879474/pregnancy-related-fractures-a-retrospective-study-of-a-french-cohort-of-52-patients-and-review-of-the-literature
#6
M Laroche, M Talibart, C Cormier, C Roux, P Guggenbuhl, Y Degboe
A retrospective, multicentre study involving 52 patients was carried out to define the causes and characteristics of pregnancy-related osteoporosis. The mean number of vertebral fractures occurring during the last trimester of pregnancy or at the time of delivery was 3.8. This is often promoted by risk factors before or during pregnancy. INTRODUCTION: In order to define the causes or predisposing factors of pregnancy-related osteoporosis and its clinical, radiological and bone density characteristics, laboratory findings, course and outcome, we carried out a retrospective multicentre study...
September 6, 2017: Osteoporosis International
https://www.readbyqxmd.com/read/28865859/hypocalcemia-cow-level-prevalence-and-preventive-strategies-in-german-dairy-herds
#7
P L Venjakob, S Borchardt, W Heuwieser
Hypocalcemia around calving is considered a gateway disease that can lead to health disorders and decreased milk production. The objective of this cross-sectional study was to evaluate the prevalence of clinical and subclinical hypocalcemia 0 to 48 h after calving. Blood samples were drawn from 12 animals of each dairy farm (n = 115) and analyzed for serum calcium, magnesium, and phosphorus concentration. Cows not affected clinically but with a serum calcium concentration below 2.0 mmol/L were characterized as subclinical hypocalcemic animals...
August 30, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28860954/distinct-clinical-outcomes-between-paramedullary-and-extramedullary-lesions-in-newly-diagnosed-multiple-myeloma
#8
Khishigjargal Batsukh, Sung-Eun Lee, Gi June Min, Sung Soo Park, Young-Woo Jeon, Jae-Ho Yoon, Byung-Sik Cho, Ki-Seong Eom, Yoo-Jin Kim, Hee-Je Kim, Seok Lee, Seok-Goo Cho, Dong-Wook Kim, Jong Wook Lee, Woo-Sung Min, Chang-Ki Min
This retrospective study aimed to compare the clinical features of paramedullary lesions (PLs) and extramedullary lesions (ELs) of plasmacytomas at diagnosis, using positron emission tomography integrated with computed tomography, using glucose labeled with the positron-emitting radionuclide (18)F ((18)F-FDG-PET/CT) in newly diagnosed multiple myeloma (NDMM), and to address their prognostic impact. Sixty-four patients with NDMM presenting ELs (n=22) and/or PLs (n=42) were included. Patients with ELs at initial presentation had unfavorable laboratory parameters of calcium and lactate dehydrogenase, a higher percentage of bone marrow plasma cells, and showed a trend toward advanced international staging system (ISS), compared to patients with PLs...
August 2017: Immune Network
https://www.readbyqxmd.com/read/28831560/a-homofermentative-bacillus-sp-bc-001-and-its-performance-as-a-potential-l-lactate-industrial-strain
#9
Sitanan Thitiprasert, Kentaro Kodama, Somboon Tanasupawat, Phatthanon Prasitchoke, Tanapawarin Rampai, Budsabathip Prasirtsak, Vasana Tolieng, Jiraporn Piluk, Suttichai Assabumrungrat, Nuttha Thongchul
Bacillus sp. BC-001 was first reported as a potent thermotolerant and homofermentative strain for an industrial-scale L-lactate production. In a flask culture, this isolate fermented both glucose and sucrose to lactate with high yield (0.96 and 0.87 g/g) and productivity (2.8 and 2.6 g/L h), respectively. The higher lactate production performance was obtained in the simultaneous saccharification and fermentation of liquefied starch (150.1 g/L final titer, 0.98 g/g yield, 3.2 g/L h productivity) and the fed-batch glucose fermentation (139...
August 22, 2017: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/28831232/effect-of-paddy-straw-plus-nonforage-fiber-sources-based-complete-rations-with-different-levels-of-neutral-detergent-fiber-on-hemato-biochemical-and-mineral-profile-of-lactating-dairy-cows
#10
Biju Chacko, K M Syam Mohan, K Ally, K Shyama, K S Anil, C T Sathian
AIM: This study was conducted to assess the effect of feeding paddy straw plus nonforage fiber sources based complete rations with different levels of neutral detergent fiber (NDF) on hemato-biochemical and mineral parameters of lactating dairy cows. MATERIALS AND METHODS: The study was conducted for 6 months in 18 lactating dairy cows, divided into three groups of six each, by feeding them on paddy straw plus nonforage fiber sources based complete rations containing different levels of NDF, in two phases of 3 months (90 days) each, being the early and mid lactation phases, respectively...
July 2017: Veterinary World
https://www.readbyqxmd.com/read/28820965/systematic-identification-of-mcu-modulators-by-orthogonal-interspecies-chemical-screening
#11
Daniela M Arduino, Jennifer Wettmarshausen, Horia Vais, Paloma Navas-Navarro, Yiming Cheng, Anja Leimpek, Zhongming Ma, Alba Delrio-Lorenzo, Andrea Giordano, Cecilia Garcia-Perez, Guillaume Médard, Bernhard Kuster, Javier García-Sancho, Dejana Mokranjac, J Kevin Foskett, M Teresa Alonso, Fabiana Perocchi
The mitochondrial calcium uniporter complex is essential for calcium (Ca(2+)) uptake into mitochondria of all mammalian tissues, where it regulates bioenergetics, cell death, and Ca(2+) signal transduction. Despite its involvement in several human diseases, we currently lack pharmacological agents for targeting uniporter activity. Here we introduce a high-throughput assay that selects for human MCU-specific small-molecule modulators in primary drug screens. Using isolated yeast mitochondria, reconstituted with human MCU, its essential regulator EMRE, and aequorin, and exploiting a D-lactate- and mannitol/sucrose-based bioenergetic shunt that greatly minimizes false-positive hits, we identify mitoxantrone out of more than 600 clinically approved drugs as a direct selective inhibitor of human MCU...
August 17, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28815389/spine-bone-mineral-density-increases-after-6%C3%A2-months-of-exclusive-lactation-even-in-women-who-keep-breastfeeding
#12
Sandra Cooke-Hubley, Beth J Kirby, James E Valcour, Gerald Mugford, Jonathan D Adachi, Christopher S Kovacs
This pilot study enrolled 31 women who had breastfed exclusively for 6 months. Lumbar and thoracic BMD increased 4 and 5%, respectively. Femoral neck and total body BMD did not change. Return of menses and progestin-only pill use were two potential signals that predicted a greater increase in BMD. PURPOSE/INTRODUCTION: The skeleton is resorbed during lactation to provide much of the calcium content of milk. After lactation ceases, these deficits in skeletal mineral content are largely restored, such that lactation has a neutral or protective effect against the long-term risk of low bone mineral density (BMD), osteoporosis, and fragility fractures...
August 16, 2017: Archives of Osteoporosis
https://www.readbyqxmd.com/read/28808392/antiurolithiatic-effect-of-sirupeelai-samoola-kudineer-a-polyherbal-siddha-decoction-on-ethylene-glycol-induced-renal-calculus-in-experimental-rats
#13
A Hannah Rachel Vasanthi, V Muthulakshmi, V Gayathri, R Manikandan, S Ananthi, Sarah Kuruvilla
BACKGROUND: Sirupeelai Samoola Kudineer (SK), a polyherbal decoction containing four medicinal plants has been used in Siddha system of medicine, practiced in Southern parts of India for the management of urolithiasis. OBJECTIVE: The present study is carried out to scientifically validate the traditional claim and to study the mechanism of action of the drug. MATERIALS AND METHODS: In the present study, anti-urolithiatic effect of SK was evaluated in Sprague-Dawley rats using ethylene glycol through drinking water and intraperitoneal injection of sodium oxalate...
July 2017: Pharmacognosy Magazine
https://www.readbyqxmd.com/read/28802019/effects-of-ultrasound-treatment-on-physicochemical-functional-properties-and-antioxidant-activity-of-whey-protein-isolate-in-the-presence-of-calcium-lactate
#14
Zhanmei Jiang, Kun Yao, Xiangying Yuan, Zhishen Mu, Zengli Gao, Juncai Hou, Lianzhou Jiang
BACKGROUND: The aim of this study was to investigate the effects of ultrasound applied at various powers (0, 200, 400, 600 or 800 W) and for different times (20 or 40 min) on the physicochemical, functional properties and antioxidant activities of whey protein isolate (WPI) dispersions in the presence of 1.20 mmol L(-1) calcium lactate. RESULTS: Surface hydrophobicity and free sulfhydryl group of the WPI dispersions containing 1.2 mmol L(-1) calcium lactate were significantly enhanced after sonication...
August 12, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28777252/n-methyl-d-aspartate-receptor-nmdar-driven-calcium-influx-potentiates-the-adverse-effects-of-myocardial-ischemia-reperfusion-injury-ex-vivo
#15
Zi-You Liu, Shou Hu, Qin-Wen Zhong, Cheng-Nan Tian, Hou-Mou Ma, Jun-Jian Yu
BACKGROUND: Despite the adverse effects of N-methyl-D-aspartate receptor (NMDAR) activity in cardiomyocytes, no study has yet examined the effects of NMDAR activity under ex vivo ischemic-reperfusion (I/R) conditions. Therefore, our aim was to comprehensively evaluate the effects of NMDAR activity through an ex vivo myocardial I/R rat model. METHODS: Isolated rat hearts were randomly segregated into six groups (n=20 in each group): (i) an untreated control group; (ii) a NMDA-treated control group; (iii) an untreated I/R group; (iv) an I/R+NMDA group treated with NMDA; (v) an I/R+NMDA+MK-801 group treated with NMDA and the NMDAR inhibitor MK-801; and (vi) an I/R+NMDA+[Ca]-free group treated with NMDA and [Ca]-free buffer...
August 4, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28772594/in-vitro-evaluation-of-dentin-tubule-occlusion-for-novel-calcium-lactate-phosphate-clp-paste
#16
Jen-Chang Yang, Hsin-Tai Hu, Sheng-Yang Lee, Sung-Chih Hsieh, Pei-Chi Huang, Chen-Feng Ma, Dian-Yu Ji, Liang-Yu Chang, Nai-Chia Teng
INTRODUCTION: The objective of this in vitro study is to evaluate the effective and long-term occlusion of dentinal tubules using a novel calcium lactate phosphate (CLP) based desensitizing agent. METHODS: Dentin disks (n = 9) were pre-etched using 1 M lactic acid for 30 s and individually treated with Colgate(®) Pro-Relief™ paste, CLP paste, and double distilled water (ddH₂O) by a rubber-cupped handpiece. Dentin disks were analyzed under optical micrographs for pre-treatment, directly after treatment, and 14 days post-treatment...
February 27, 2017: Materials
https://www.readbyqxmd.com/read/28772474/optimization-of-a-binary-concrete-crack-self-healing-system-containing-bacteria-and-oxygen
#17
Jinlong Zhang, Bixia Mai, Tingwei Cai, Jiayi Luo, Wanhan Wu, Bing Liu, Ningxu Han, Feng Xing, Xu Deng
An optimized strategy for the enhancement of microbially induced calcium precipitation including spore viability ensurance, nutrient selection and O2 supply was developed. Firstly, an optimal yeast extract concentration of 5 g/l in sporulation medium was determined based on viable spore yield and spore viability. Furthermore, the effects of certain influential factors on microbial calcium precipitation process of H4 in the presence of oxygen releasing tablet (ORT) were evaluated. The results showed that CaO2 is preferable to other peroxides in improving the calcium precipitation by H4...
January 26, 2017: Materials
https://www.readbyqxmd.com/read/28756148/ginsenoside-rg2-protects-pc12%C3%A2-cells-against-%C3%AE-amyloid25-35-induced-apoptosis-via-the-phosphoinositide-3-kinase-akt-pathway
#18
Jing Cui, Jiao Wang, Meizhu Zheng, Dongxia Gou, Chunming Liu, Yifa Zhou
Alzheimer's disease (AD) is one of the most debilitating neurodegenerative diseases in an aging population. Excessive accumulation of β-amyloid (Aβ) has been proposed as a pivotal event in the pathogenesis of AD. Ginsenoside Rg2 has been reported to exert neuroprotective effects. However, the underlying mechanism for its neuroprotection is not well-understood. In this study, we investigated the protective effects of ginsenoside Rg2 on Aβ25-35-induced neurotoxicity in PC12 cells and identified a potential molecular signaling pathway involved...
July 26, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28752512/medical-treatment-guidelines-for-preventive-treatment-of-migraine
#19
Tzu-Chou Huang, Tzu-Hsien Lai, Treatment Guideline Subcommittee Of Taiwan Headache Society Taiwan Headache Society
The Treatment Guideline Subcommittee of the Taiwan Headache Society evaluated the medications currently used for migraine prevention in Taiwan. We assessed the results of new published drug trials, information from medical database and referred to the latest guidelines published. After comprehensive discussion, we proposed Taiwanese consensus about the preventive treatment for migraine including recommendation levels, strength of evidences, and related prescription information regarding dosage and adverse effects...
March 15, 2017: Acta Neurologica Taiwanica
https://www.readbyqxmd.com/read/28725115/maternal-and-fetal-arterial-blood-gas-data-in-normotensive-singleton-isoflurane-anesthetized-sheep-at-124-126-days-of-gestation
#20
Claire M Loughran, Matthew W Kemp, Gabrielle C Musk
The aim of this case series was to describe the differences between maternal and fetal blood-gas results during anesthesia. Sixteen singleton adult merino ewes weighing 60.1 ± 5.1 kg at 125.7 d (124 to 126 d) gestation were anesthetized. Maternal (radial) and fetal (umbilical) arterial blood gas samples were collected 79 ± 6 min after the start of anesthesia if maternal mean arterial pressure (MAP) was stable and > 65 mmHg. Fetal pH, partial arterial pressure of oxygen (PaO2), glucose, arterial hemoglobin oxygen saturation (SaO2), sodium, and chloride were significantly lower and fetal partial arterial pressure of carbon dioxide (PaCO2), lactate, hematocrit, total hemoglobin, potassium, and calcium were significantly higher than maternal blood-gas values...
July 2017: Canadian Journal of Veterinary Research, Revue Canadienne de Recherche Vétérinaire
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