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https://www.readbyqxmd.com/read/29045397/glucose-feeds-the-tca-cycle-via-circulating-lactate
#1
Sheng Hui, Jonathan M Ghergurovich, Raphael J Morscher, Cholsoon Jang, Xin Teng, Wenyun Lu, Lourdes A Esparza, Tannishtha Reya, Le Zhan, Jessie Yanxiang Guo, Eileen White, Joshua D Rabinowitz
Mammalian tissues are fuelled by circulating nutrients, including glucose, amino acids, and various intermediary metabolites. Under aerobic conditions, glucose is generally assumed to be burned fully by tissues via the tricarboxylic acid cycle (TCA cycle) to carbon dioxide. Alternatively, glucose can be catabolized anaerobically via glycolysis to lactate, which is itself also a potential nutrient for tissues and tumours. The quantitative relevance of circulating lactate or other metabolic intermediates as fuels remains unclear...
October 18, 2017: Nature
https://www.readbyqxmd.com/read/29045028/biological-effects-of-er-yag-laser-irradiation-on-the-proliferation-of-primary-human-gingival-fibroblasts
#2
Sophannary Kong, Akira Aoki, Kengo Iwasaki, Koji Mizutani, Sayaka Katagiri, Tomonari Suda, Shizuko Ichinose, Mayumi Ogita, Verica Pavlic, Yuichi Izumi
We investigated the biological effects of Er:YAG laser (2,940-nm; DELight(®) , HOYA ConBio, USA) irradiation at fluences of 3.6, 4.2, 4.9, 6.3, 8.1, or 9.7 J cm(-2) at 20 or 30 Hz for 20 or 30 s on primary human gingival fibroblasts (HGFs). Irradiation at 6.3 J cm(-2) promoted maximal cell proliferation, determined by WST-8 assay and crystal violet staining, but was accompanied by lactate dehydrogenase release, on day 3 post-irradiation. Elevation of ATP level, Ki67 staining, and cyclin-A2 mRNA expression confirmed that Er:YAG affected the cell cycle and increased the number of proliferating cells...
October 17, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/29043982/preanalytical-influence-of-pneumatic-tube-delivery-system-on-results-of-routine-biochemistry-and-haematology-analysis
#3
Morgane Petit, Louis Mine, Tiffany Pascreau, Chantal Brouzes, Sandrine Majoux, Delphine Borgel, Jean-Louis Beaudeux, Dominique Lasne, Carole Hennequin
Pneumatic tube delivery system (PTS) enables to reduce considerably turnaround times. The aim of the study was to assess the influence of the PTS on the quality of routine biochemical and hematological tests in our laboratory. Blood samples from 6 hospitalized patients and 8 healthy volunteers were analyzed. Blood samples were delivered to the laboratory by a PTS and by a human courier. We performed the following analysis: ionized calcium, sodium, potassium, lactate deshydrogenase (LDH), aspartate aminotransferase (ASAT), arterial blood gas, complete blood count and coagulation test as prothrombin time, activated partial thromboplastin time, factors V and VIII...
October 18, 2017: Annales de Biologie Clinique
https://www.readbyqxmd.com/read/29043796/composite-hydrogels-containing-bioactive-microreactors-for-optical-enzymatic-lactate-sensing
#4
Aniket Biswas, Lindsey Bornhoeft, Swayoma Banerjee, Yilhwan You, Michael J McShane
Continuously monitoring specific biomarkers offer a promising method to interrogate disease status and progression. In this work we have demonstrated a nanocomposite hydrogel-based sensing platform that may be used for optical detection of lactate. The sensor design consists of micro-sized enzymatic sensors that are embedded in an outer hydrogel matrix. In these engineered microdomains, encapsulated lactate oxidase serves as the bioactive component, phosphorescent palladium tetracarboxyphenyl porphyrin acts as the optical transducer, and polyelectrolyte multilayers coated on the enzymatic micro-sensors controls the permeation of lactate into the micro-sensors...
October 18, 2017: ACS Sensors
https://www.readbyqxmd.com/read/29043406/predictive-value-of-scvo2-monitoring-for-pericardial-tamponade-after-cardiac-surgery
#5
S Saha, H Baraki, I Kutschka, J Hadem
BACKGROUND: We examined the predictive value of central venous oxygen saturation (ScvO2) changes regarding the occurrence of pericardial tamponade following cardiac surgery. METHODS: We retrospectively identified 66 consecutive patients in whom ScvO2 and arterial lactate levels were analyzed during an 8‑h time interval preceding pericardiotomy due to pericardial tamponade (PT), and at equivalent time points in 30 control patients (C) who had an uncomplicated course...
October 17, 2017: Herz
https://www.readbyqxmd.com/read/29043143/focal-segmental-glomerulosclerosis-associated-with-mitochondrial-disease
#6
Kenneth Lim, David Steele, Andrew Fenves, Ravi Thadhani, Eliot Heher, Amel Karaa
Primary mitochondrial diseases (MD) are complex, heterogeneous inherited diseases caused by mutations in either the mitochondrial or nuclear DNA. Glomerular diseases in MD have been reported with tRNA mutation m.3243A>G causing a syndrome of mitochondrial encephalomyopathy, lactic acidosis and stroke-like episodes (MELAS). We describe here a case of focal segmental glomerulosclerosis (FSGS) associated with a new tRNA mutation site. A 34-year-old man with a history of living related kidney transplantation, diabetes, hearing loss, and developmental delay presented to the outpatient clinic with complaints of new behavioral difficulties, worsening symptoms, and brain involvement on imaging...
2017: Clin Nephrol Case Stud
https://www.readbyqxmd.com/read/29043013/mir-125a-suppresses-viability-and-glycolysis-and-induces-apoptosis-by-targeting-hexokinase-2-in-laryngeal-squamous-cell-carcinoma
#7
Zhanwei Sun, Wenqi Zhang, Qian Li
BACKGROUND: miR-125a usually functions as a tumor suppressor in cancers. However, the role of miR-125a in laryngeal squamous cell carcinoma (LSCC) has not been determined. METHODS: qRT-PCR was applied to measure the expression of miR-125a and HK2 mRNA in LSCC tissues and cells. CCK-8 kit and flow cytometry analysis were performed to detect cell viability and apoptosis. Luciferase reporter assay and RNA immunoprecipitation (RIP) were conducted to confirm the relationship between miR-125a and HK2...
2017: Cell & Bioscience
https://www.readbyqxmd.com/read/29042839/a-retrospective-study-on-infusion-related-reactions-to-rituximab-in-a-heterogeneous-pediatric-population
#8
Clément Legeay, Henrique Bittencourt, Elie Haddad, Laurence Spiesser-Robelet, Valérie Thépot-Seegers, Roxane Therrien
OBJECTIVES: To assess risks and outcomes of infusion-related reactions to rituximab in a heterogeneous pediatric population. METHODS: All patients who received rituximab between July 2010 and July 2012 were retrieved from the pharmacy software and included for analysis. Data were collected according to 4 categories: demographic data, infusion data, infusion-related reactions, and biological data considered as risk factors (i.e., absolute lymphocyte count, lactate dehydrogenase levels)...
September 2017: Journal of Pediatric Pharmacology and Therapeutics: JPPT: the Official Journal of PPAG
https://www.readbyqxmd.com/read/29040016/centella-asiatica-fraction-3-suppresses-the-nuclear-factor-erythroid-2-related-factor-2-anti-oxidant-pathway-and-enhances-reactive-oxygen-species-mediated-cell-death-in-cancerous-lung-a549-cells
#9
Dhaneshree Bestinee Naidoo, Alisa Phulukdaree, Krishnan Anand, Vikash Sewram, Anil Amichund Chuturgoon
Centella asiatica is a tropical medicinal plant that is commonly used in traditional medicine. Medicinal properties of C. asiatica include anti-oxidant, anti-inflammatory, and anti-cancer activity. We investigated the anti-oxidant and anti-proliferative/cytotoxic effects of a semi-purified fraction of C. asiatica ethanolic leaf extract (C3) in cancerous lung A549 cells. C3 was obtained by silica column fractionation and identified by using thin-layer chromatography and gas chromatography mass spectrometry. Cytotoxicity of C3 in A549 cells was evaluated (cell viability assay-WST-1; 24 h; [0...
October 2017: Journal of Medicinal Food
https://www.readbyqxmd.com/read/29040000/methionine-induces-lat1-expression-in-dairy-cow-mammary-gland-by-activating-the-mtorc1-signaling-pathway
#10
Xiaoyu Duan, Ye Lin, He Lv, Yang Yang, Hongtao Jiao, Xiaoming Hou
Methionine is the limiting amino acid for milk protein synthesis in dairy cows. The effect of methionine availability on milk protein synthesis is dependent on its active transport into cells through amino acid transporters. L-type amino acid transporter 1 (LAT1), which induces the transport of neutral amino acids, is highly expressed in lactating mammary gland. However, the effect of methionine on LAT1 expression and the mechanism governing this process in dairy cow mammary gland are poorly understood. In this study, we show that treatment of dairy cow mammary epithelial cells with increasing concentrations of methionine for 24 h resulted in increased expression of LAT1 and its associated protein 4F2 heavy chain (4F2hc)...
October 17, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/29039534/apurinic-apyrimidinic-endonuclease-1-ape1-contributes-to-resveratrol%C3%A2-induced-neuroprotection-against-oxygen%C3%A2-glucose-deprivation-and-re%C3%A2-oxygenation-injury-in-ht22-cells-involvement-in-reducing-oxidative-dna-damage
#11
Jiao-Ying Jia, Zhi-Gang Tan, Min Liu, Yu-Gang Jiang
Resveratrol, a naturally occurring polyphenolic compound, exhibits a neuroprotective role in models of central nervous system diseases, including cerebral ischemia/reperfusion injury. Apurinic/apyrimidinic endonuclease 1 (APE1) is a multifunctional enzyme that contributes to base excision repair of oxidative DNA damage and redox activation of transcription factors, associated with neuronal survival against hypoxic‑ischemic injury. It was hypothesized that resveratrol protects HT22 cells against oxygen‑glucose deprivation and re‑oxygenation (OGD/R)‑induced injuries through upregulation of APE1...
October 17, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039526/promoting-effects-of-il%C3%A2-23-on-myocardial-ischemia-and-reperfusion-are-associated-with-increased-expression-of-il%C3%A2-17a-and-upregulation-of-the-jak2%C3%A2-stat3-signaling-pathway
#12
Yanxi Liao, Xiaorong Hu, Xin Guo, Bofang Zhang, Weipan Xu, Hong Jiang
Interleukin (IL)‑23, as a novel pro‑inflammatory cytokine, is important in several inflammatory diseases, including myocardial ischemia and reperfusion (I/R) injury, however, the underlying mechanism remains to be elucidated. The present study was designed to investigate the specific role of IL‑23 in myocardial I/R injury, and whether the Janus kinase 2/signal transducer and activator of transcription 3 (JAK2‑STAT3) signaling pathway, one of the important downstream signaling pathways of IL‑23, and the IL‑17A downstream pro‑inflammatory cytokine, were involved...
October 12, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039499/an-investigation-of-methyl-tert%C3%A2-butyl-ether%C3%A2-induced-cytotoxicity-and-protein-profile-in-chinese-hamster-ovary-cells
#13
Guangshan Xie, Wen-Xu Hong, Li Zhou, Xifei Yang, Haiyan Huang, Desheng Wu, Xinfeng Huang, Weiguo Zhu, Jianjun Liu
Methyl tert-butyl ether (MTBE) is widely used as an oxygenating agent in gasoline to reduce harmful emissions. However, previous studies have demonstrated that MTBE is a cytotoxic substance that has harmful effects in vivo and in vitro. Although remarkable progress has been made in elucidating the mechanisms underlying the MTBE‑induced reproductive toxicological effect in different cell lines, the precise mechanisms remain far from understood. The present study aimed to evaluate whether mammalian ovary cells were sensitive to MTBE exposure in vitro by assessing cell viability, lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and antioxidant enzyme activities...
October 11, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29039018/the-activity-demands-and-physiological-responses-encountered-during-basketball-match-play-a-systematic-review
#14
REVIEW
Emilija Stojanović, Nenad Stojiljković, Aaron T Scanlan, Vincent J Dalbo, Daniel M Berkelmans, Zoran Milanović
BACKGROUND: Basketball is a popular, court-based team sport that has been extensively studied over the last decade. OBJECTIVE: The purpose of this article was to provide a systematic review regarding the activity demands and physiological responses experienced during basketball match-play according to playing period, playing position, playing level, geographical location, and sex. METHODS: An electronic database search of relevant articles published prior to 30 September 2016 was performed with PubMed, MEDLINE, ERIC, Google Scholar, SCIndex, and ScienceDirect...
October 16, 2017: Sports Medicine
https://www.readbyqxmd.com/read/29037410/heat-adaptation-from-regular-hot-water-immersion-decreases-proinflammatory-responses-hsp70-expression-and-physical-heat-stress
#15
Fwu-Lin Yang, Chia-Chi Lee, Yi-Maun Subeq, Chung-Jen Lee, Chun-Yen Ke, Ru-Ping Lee
Hot-water immersion (HWI) is a type of thermal therapy for treating various diseases. In our study, the physiological responses to occasional and regular HWI have been explored. The rats were divided into a control group, occasional group (1D), and regular group (7D). The 1D and 7D groups received 42°C during 15mins HWI for 1 and 7 days, respectively. The blood samples were collected for proinflammatory cytokines examinations, the heart, liver and kidney were excised for subsequent IHC analysis to measure the level of heat shock protein 70 (HSP70)...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29037390/effect-of-feeding-rumen-protected-capsule-containing-niacin-k2so4-vitamin-c-and-gamma-aminobutyric-acid-on-heat-stress-and-performance-of-dairy-cows
#16
W J Guo, L Zhen, J X Zhang, S Lian, H F Si, J R Guo, H M Yang
This study was conducted to evaluate the effects of supplemental rumen-protected capsule (RPC) on animal performance, serological indicators, and serum heat shock protein 70 (HSP70) of lactating Holstein cows under heat stress (HS). During summer months, 30 healthy multiparous lactating Holstein cows with a parity number of 3.1 ± 0.44, 70 ± 15 d in milk, an average body weight of 622 ± 62kg, and an average milk yield of 32.28 ± 0.96kg/d, were used. The cows were randomly allocated to two groups: a control group and an RPC-supplemented group (0...
October 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/29036843/aberrant-peak-lactate-response-in-ms
#17
Kathrin Schlüter, Josephina Maier, Stefan Patra, Stefan M Gold, Christoph Heesen, Karl-Heinz Schulz
BACKGROUND: The peak blood lactate response to an exhaustive exercise test in a number of chronic conditions has been shown to differ from that seen in healthy, untrained individuals. However, this has not been investigated for patients with multiple sclerosis (MS). OBJECTIVE: The main objective was to determine and compare the peak blood lactate response to exercise and the maximal workload between two groups of MS patients with different illness severity. METHODS: Twenty-five patients with a relapsing-remitting disease course (Group RR) and 41 patients with a secondary- or primary chronic progressive disease course (group CP) performed an exhaustive incremental bicycle ergometry...
October 11, 2017: NeuroRehabilitation
https://www.readbyqxmd.com/read/29035870/oxidative-stress-and-cytotoxicity-elicited-lipid-peroxidation-in-hemocytes-of-bombyx-mori-larva-infested-with-dipteran-parasitoid-exorista-bombycis
#18
Makwana Pooja, Appukuttan Nair R Pradeep, Shambhavi P Hungund, Chandrashekhar Sagar, Kangayam M Ponnuvel, Arvind K Awasthi, Kanika Trivedy
Parasitization of silkworm, Bombyx mori by invasive larva of dipteran parasitoid Exorista bombycis caused upto 20% revenue loss in sericulture. The parasitism was successful by suppressing host immune system however mechanism of immune suppression induced by E. bombycis is unknown which is unravelled here. The infestation induced cytotoxic symptoms in host hemocytes, such as vacuolated cytoplasm, porous plasma membrane, indented nuclei with condensed chromatin and dilated RER. One of the markers of necrosis is cell permeabilization, which can be measured as released lactate dehydrogenase (LDH)...
December 20, 2017: Acta Parasitologica
https://www.readbyqxmd.com/read/29035826/effects-of-gallic-acid-on-hemodynamic-parameters-and-infarct-size-after-ischemia-reperfusion-in-isolated-rat-hearts-with-alloxan-induced-diabetes
#19
Fatemeh Ramezani-Aliakbari, Mohammad Badavi, Mahin Dianat, Seyed Ali Mard, Akram Ahangarpour
Diabetic rats are more susceptible to myocardial ischemia-reperfusion injury than control rats. The aim of the present study was to evaluate the cardioprotective effect of gallic acid (GA) on isolated rat hearts with alloxan-induced diabetes mellitus. Adult male Sprague-Dawley rats were divided randomly into three groups: control, untreated diabetic and diabetic animals treated with (GA, 25mg/kg). Diabetes was induced by 120mg/kg alloxan injection. Eight weeks after GA administration, the hearts were isolated and exposed to myocardial ischemia-reperfusion...
October 13, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29034747/long-term-supplementation-of-rhodiola-kirilowii-extracts-during-pregnancy-and-lactation-does-not-affect-mother-health-status
#20
Sławomir Lewicki, Ewa Skopińska-Różewska, Aneta Lewicka, Robert Zdanowski
Antibiotics treatment during pregnancy and lactation is problematic. The alternative to the antibiotic treatment is the use of plant-derived supplements, which stimulate immune system to prevent and eliminate bacterial infection. Here we evaluated the effect of long-term use of Rhodiola kirilowii on the health of mouse mothers. Pregnant mice were fed daily, for whole pregnancy and for 28 days after giving birth, with Rhodiola kirilowii water (RKW) or hydroalcoholic extract (RKW-A) (at 20 mg of extracts/kg)...
October 16, 2017: Journal of Maternal-fetal & Neonatal Medicine
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