keyword
MENU ▼
Read by QxMD icon Read
search

Genetics of MRSA

keyword
https://www.readbyqxmd.com/read/27902380/antimicrobial-resistance-profiling-and-molecular-typing-of-methicillin-resistant-staphylococcus-aureus-mrsa-isolated-from-a-malaysian-teaching-hospital
#1
Ainihayati Noordin, Hassriana Fazzila Sapri, Nurul Azirah Mohamad Sani, Shih Kei Leong, Xin-Ee Tan, Toh Leong Tan, Noraziah Mohamad Zin, Hui-Min Neoh, Salasawati Hussin
The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be at 30-40% of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of our teaching hospital located in Kuala Lumpur, the capital city of Malaysia, and performed antimicrobial susceptibility testing on the strains. The strains were then molecularly characterized via SCCmec and virulence gene (cna, sea, seb, sec, sed, see, seg, seh, sei, eta, etb, PVL and TSST-1) typing; a subset of 49 strains isolated from the Intensive Care Unit (ICU) were also typed using Pulsed-Field Gel Electrophoresis (PFGE)...
November 7, 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27899074/antibiotic-resistance-and-clonal-diversity-of-invasive-staphylococcus-aureus-in-the-rural-ashanti-region-ghana
#2
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May
BACKGROUND: Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. METHODS: Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S...
November 29, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27895635/molecular-characteristic-and-virulence-gene-profiles-of-community-associated-methicillin-resistant-staphylococcus-aureus-isolates-from-pediatric-patients-in-shanghai-china
#3
Xing Wang, Xia Li, Wei Liu, Weichun Huang, Qihua Fu, Min Li
Staphylococcus aureus is a globally important human pathogen, especially among children and immunocompromised patients. The emergence and spread of community-associated methicillin-resistant S. aureus (CA-MRSA) has become a serious public health problem worldwide. The aim of this study was to investigate the prevalence, molecular characteristics and virulence profiles of CA-MRSA infections from pediatric patients in a university hospital in Shanghai, China. A total of 80 CA-MRSA isolates were collected from July 2012 to December 2013 in Shanghai Children's Medical Center and analyzed by multilocus sequence typing, staphylococcus chromosomal cassette mec (SCCmec) typing, and spa typing...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27881973/livestock-associated-methicillin-resistant-staphylococcus-aureus-la-mrsa-clonal-complex-cc-398-isolated-from-uk-animals-belong-to-european-lineages
#4
Meenaxi Sharma, Javier Nunez-Garcia, Angela M Kearns, Michel Doumith, Patrick R Butaye, M Angeles Argudín, Angela Lahuerta-Marin, Bruno Pichon, Manal AbuOun, Jon Rogers, Richard J Ellis, Christopher Teale, Muna F Anjum
In recent years, there has been an increase in the number of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 recovered from S. aureus isolated animals in the UK. To determine possible origins of 12 LA-MRSA CC398 isolates collected after screening more than a thousand S. aureus animal isolates from the UK between 2013 and 2015, whole genome sequences (WGS) of CC398 European, including UK, and non-European isolates from diverse animal hosts were compared. Phylogenetic reconstruction applied to WGS data to assess genetic relatedness of all 89 isolates, clustered the 12 UK CC398 LA-MRSA within the European sub-lineages, although on different nodes; implicating multiple independent incursions into the UK, as opposed to a single introduction followed by clonal expansion...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27869532/molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-outpatients-in-northern-japan-increasing-tendency-of-st5-st764-mrsa-iia-with-arginine-catabolic-mobile-element
#5
Meiji Soe Aung, Mitsuyo Kawaguchiya, Noriko Urushibara, Ayako Sumi, Masahiko Ito, Kenji Kudo, Shigeo Morimoto, Shino Hosoya, Nobumichi Kobayashi
Arginine catabolic mobile element (ACME) is a genomic island of staphylococcus and is considered to confer enhanced ability to survive and growth on host bacterial cells. ACME has been typically identified in Panton-Valentine Leukocidin (PVL)-positive ST8 methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type IVa (USA300 clone), and it is also found in other lineages at low frequency. Prevalence and molecular characteristics of PVL(+)and/or ACME(+) MRSA were investigated for 624 clinical isolates collected from outpatients in northern Japan from 2013 to 2014...
November 21, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27865508/occurrence-of-methicillin-resistant-staphylococcus-aureus-in-dairy-cattle-herds-related-swine-farms-and-humans-in-contact-with-herds
#6
C Locatelli, P Cremonesi, A Caprioli, V Carfora, A Ianzano, A Barberio, S Morandi, A Casula, B Castiglioni, V Bronzo, P Moroni
In this study we investigated the circulation of methicillin-resistant Staphylococcus aureus (MRSA) in 2 dairy cattle farms (farm A and B), previously identified as MRSA-positive in bulk tank milk samples, and epidemiologically related to swine farms. Collected specimens included quarter milk samples and nasal swabs from dairy cows, pig nasal swabs collected at both the farm and slaughterhouse level, environmental dust samples, and human nasal swabs from the farms' owners and workers. The prevalence of MRSA was estimated at the herd level by testing quarter milk samples...
November 16, 2016: Journal of Dairy Science
https://www.readbyqxmd.com/read/27855652/performance-of-an-electronic-health-record-based-phenotype-algorithm-to-identify-community-associated-methicillin-resistant-staphylococcus-aureus-cases-and-controls-for-genetic-association-studies
#7
Kathryn L Jackson, Michael Mbagwu, Jennifer A Pacheco, Abigail S Baldridge, Daniel J Viox, James G Linneman, Sanjay K Shukla, Peggy L Peissig, Kenneth M Borthwick, David A Carrell, Suzette J Bielinski, Jacqueline C Kirby, Joshua C Denny, Frank D Mentch, Lyam M Vazquez, Laura J Rasmussen-Torvik, Abel N Kho
BACKGROUND: Community associated methicillin-resistant Staphylococcus aureus (CA-MRSA) is one of the most common causes of skin and soft tissue infections in the United States, and a variety of genetic host factors are suspected to be risk factors for recurrent infection. Based on the CDC definition, we have developed and validated an electronic health record (EHR) based CA-MRSA phenotype algorithm utilizing both structured and unstructured data. METHODS: The algorithm was validated at three eMERGE consortium sites, and positive predictive value, negative predictive value and sensitivity, were calculated...
November 17, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27851997/mrsa-among-animals-current-overview
#8
REVIEW
Marta Aires-de-Sousa
Currently, methicillin-resistant Staphylococcus aureus (MRSA) is a universal threat. After being well established in the healthcare setting, it has emerged in the community among people with no risk factors for MRSA acquisition, therefore imposing a new threat. The subsequent detection of MRSA colonizing or infecting animals as well as in food of animal origin was of major concern, revealing new reservoirs for MRSA. The major MRSA clonal lineages circulating in the different settings, i.e. in hospitals, in the community and among animals are described here, differentiating between clones colonizing companion and food-chain animals...
November 13, 2016: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/27836760/staphylococcal-chromosomal-cassettes-mec-sccmec-a-mobile-genetic-element-in-methicillin-resistant-staphylococcus-aureus
#9
REVIEW
Junyan Liu, Dingqiang Chen, Brian M Peters, Lin Li, Bing Li, Zhenbo Xu, Mark E Shirliff
Considered to be a potential "superbug", methicillin-resistant Staphylococcus aureus (MRSA) has been one of the major recent infectious pathogens and thus poses a challenge to hospital infection control. The mobile genetic element staphylococcal chromosomal cassette mec (SCCmec) carries both the mecA or mecC gene, encoding for a novel specific penicillin-binding protein (PBP2a), and site-specific recombinase genes ccrAB or/and ccrC. In MRSA, the acquisition of SCCmec leads to the resistance to the β-lactam antibiotics...
November 9, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27827853/antimicrobial-usage-and-antimicrobial-resistance-in-animal-production-in-southeast-asia-a-review
#10
REVIEW
Nguyen T Nhung, Nguyen V Cuong, Guy Thwaites, Juan Carrique-Mas
Southeast Asia is an area of great economic dynamism. In recent years, it has experienced a rapid rise in the levels of animal product production and consumption. The region is considered to be a hotspot for infectious diseases and antimicrobial resistance (AMR). We reviewed English-language peer-reviewed publications related to antimicrobial usage (AMU) and AMR in animal production, as well as antimicrobial residues in meat and fish from 2000 to 2016, in the region. There is a paucity of data from most countries and for most bacterial pathogens...
November 2, 2016: Antibiotics
https://www.readbyqxmd.com/read/27755189/a-10-year-review-of-ocular-methicillin-resistant-staphylococcus-aureus-infections-epidemiology-clinical-features-and-treatment
#11
Emily S Wong, Claire W Y Chow, W K Luk, Kitty S C Fung, Kenneth K W Li
PURPOSE: To characterize epidemiological data on methicillin-resistant Staphylococcus aureus (MRSA) ocular infections over a 10-year period in Hong Kong; to compare the characteristics between hospital-associated methicillin-resistant Staphylococcus aureus (h-MRSA) and community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) ocular infections; and to review the treatment regimen and outcome of identified cases. METHODS: A retrospective case review of ocular samples testing positive for MRSA at a tertiary eye center from July 2005 to June 2015 was performed...
October 14, 2016: Cornea
https://www.readbyqxmd.com/read/27729903/potential-relationship-between-phenotypic-and-molecular-characteristics-in-revealing-livestock-associated-staphylococcus-aureus-in-chinese-humans-without-occupational-livestock-contact
#12
Yanping Fan, Xiaolin Wang, Ling Li, Zhenjiang Yao, Sidong Chen, Xiaohua Ye
While some studies have defined Staphylococcus aureus based on its clonal complex and resistance pattern, few have explored the relations between the genetic lineages and antibiotic resistance patterns and immune evasion cluster (IEC) genes. Our aim was to investigate the potential relationship between phenotypic and molecular characteristics so as to reveal livestock-associated S. aureus in humans. The study participants were interviewed, and they provided two nasal swabs for S. aureus analysis. All S. aureus and methicillin-resistant S...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27717648/phenotypic-characteristics-and-comparative-proteomics-of-staphylococcus-aureus-strains-with-different-vancomycin-resistance-levels
#13
Auttawit Sirichoat, Aroonlug Lulitanond, Rattiyaporn Kanlaya, Ratree Tavichakorntrakool, Aroonwadee Chanawong, Sujintana Wongthong, Visith Thongboonkerd
Reduced vancomycin susceptibility of methicillin-resistant Staphylococcus aureus (MRSA) is a worldwide problem. Unfortunately, its genetic marker and molecular mechanisms remained unknown. This study investigated differential phenotypic characteristic and protein expression profiles among three groups of MRSA isolates, including vancomycin-susceptible S. aureus (VSSA), heterogeneous vancomycin-intermediate S. aureus (hVISA) and vancomycin-intermediate S. aureus (VISA) (n = 7 isolates/group). Phenotypic characteristic revealed significant greater number of isolates with non-spreading colony in VISA as compared to both VSSA and hVISA groups...
September 17, 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27679613/high-nasal-carriage-rate-of-staphylococcus-aureus-containing-panton-valentine-leukocidin-and-edin-encoding-genes-in-community-and-hospital-settings-in-burkina-faso
#14
Abdoul-Salam Ouedraogo, Catherine Dunyach-Remy, Aimée Kissou, Soufiane Sanou, Armel Poda, Carole G Kyelem, Jérôme Solassol, Anne-Laure Bañuls, Philippe Van De Perre, Rasmata Ouédraogo, Hélène Jean-Pierre, Jean-Philippe Lavigne, Sylvain Godreuil
The objectives of the present study were to investigate the rate of S.aureus nasal carriage and molecular characteristics in hospital and community settings in Bobo Dioulasso, Burkina Faso. Nasal samples (n = 219) were collected from 116 healthy volunteers and 103 hospitalized patients in July and August 2014. Samples were first screened using CHROMagar Staph aureus chromogenic agar plates, and S. aureus strains were identified by mass spectrometry. Antibiotic susceptibility was tested using the disk diffusion method on Müller-Hinton agar...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27671067/presence-of-the-optra-gene-in-methicillin-resistant-staphylococcus-sciuri-of-porcine-origin
#15
Run Fan, Dexi Li, Yang Wang, Tao He, Andrea T Feßler, Stefan Schwarz, Congming Wu
A total of 57 methicillin-resistant Staphylococcus aureus (MRSA) isolates and 475 methicillin-resistant coagulase-negative staphylococci (MRCoNS) collected from pigs in the Guangdong province of China in 2014 were investigated for the presence of the novel oxazolidinone-phenicol resistance gene optrA The optrA gene was detected in 6.9% (n=33) of the MRCoNS, all of which were MR-Staphylococcus sciuri, but in none of the MRSA. Five optrA-carrying MR-S. sciuri also harbored the multiresistance gene cfr Pulsed-field gel electrophoresis (PFGE) and dru typing of the 33 optrA-carrying MR-S...
September 26, 2016: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27658767/characterization-of-methicillin-resistant-staphylococcus-aureus-isolates-from-fitness-centers-in-the-memphis-metropolitan-area-tennessee
#16
Nabanita Mukherjee, Irshad M Sulaiman, Pratik Banerjee
Indoor skin-contact surfaces of public fitness centers may serve as reservoirs of potential human transmission of methicillin-resistant Staphylococcus aureus (MRSA). We found a high prevalence of multidrug-resistant (MDR) MRSA of clonal complex 59 lineage harboring a variety of extracellular toxin genes from surface swab samples collected from inanimate surfaces of fitness centers in the Memphis metropolitan area, Tennessee. Our findings underscore the role of inanimate surfaces as potential sources of transmission of MDR MRSA strains with considerable genetic diversity...
December 1, 2016: American Journal of Infection Control
https://www.readbyqxmd.com/read/27650187/report-of-mecc-carrying-mrsa-in-domestic-swine
#17
Ø Angen, M Stegger, J Larsen, B Lilje, H Kaya, K S Pedersen, A Jakobsen, A Petersen, A R Larsen
OBJECTIVES: We unexpectedly identified MRSA isolates carrying mecC (mecC-MRSA) from a Danish swine farm located in eastern Zealand. The objective of the present study was to investigate the origin of these isolates and their genetic relatedness to other mecC-MRSA isolates from Zealand. METHODS: WGS was used to infer the phylogenetic relationship between 19 identified mecC-MRSA isolates from the swine farm and 34 additional epidemiologically unrelated human isolates from the same geographical region of Denmark...
September 20, 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/27648947/diversity-of-sccmec-elements-in-staphylococcus-aureus-as-observed-in-south-eastern-germany
#18
Stefan Monecke, Lutz Jatzwauk, Elke Müller, Hedda Nitschke, Katharina Pfohl, Peter Slickers, Annett Reissig, Antje Ruppelt-Lorz, Ralf Ehricht
SCCmec elements are very important mobile genetic elements in Staphylococci that carry beta-lactam resistance genes mecA/mecC, recombinase genes and a variety of accessory genes. Twelve main types and a couple of variants have yet been described. In addition, there are also other SCC elements harbouring other markers. In order to subtype strains of methicillin-resistant S. aureus (MRSA) based on variations within their SCCmec elements, 86 markers were selected from published SCC sequences for an assay based on multiplexed primer extension reactions followed by hybridisation to the specific probes...
2016: PloS One
https://www.readbyqxmd.com/read/27647271/molecular-characterization-of-staphylococcus-aureus-isolates-from-various-healthcare-institutions-in-nairobi-kenya-a-cross-sectional-study
#19
Geoffrey Omuse, Kristien Nel Van Zyl, Kim Hoek, Shima Abdulgader, Samuel Kariuki, Andrew Whitelaw, Gunturu Revathi
BACKGROUND: Staphylococcus aureus (S. aureus) has established itself over the years as a major cause of morbidity and mortality both within the community and in healthcare settings. Methicillin resistant S. aureus (MRSA) in particular has been a major cause of nosocomial infections resulting in significant increase in healthcare costs. In Africa, the MRSA prevalence has been shown to vary across different countries. In order to better understand the epidemiology of MRSA in a setting, it is important to define its population structure using molecular tools as different clones have been found to predominate in certain geographical locations...
2016: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/27638009/high-frequency-of-occupied-attb-regions-in-norwegian-staphylococcus-aureus-isolates-supports-a-two-step-mrsa-screening-algorithm
#20
H S Tunsjø, S Kalyanasundaram, M M Worren, T M Leegaard, A E F Moen
Rapid nucleic acid amplification tests for methicillin-resistant Staphylococcus aureus (MRSA) diagnostics commonly target the mec resistance gene, genes specific for S. aureus, and the integration site for the SCCmec resistance cassette, orfX. Due to poor specificity when these target genes are used individually, additional culture is required to verify positive results. The combination of these targets is useful, but the optimal algorithm may depend on the presence of the genetic markers in S. aureus isolates, as well as the prevalence of MRSA in a population...
September 9, 2016: European Journal of Clinical Microbiology & Infectious Diseases
keyword
keyword
65967
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"