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Genetics of MRSA

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https://www.readbyqxmd.com/read/28221920/prevalence-and-molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-in-foods-of-animal-origin-turkey
#1
Belgin Siiriken, Tuba Yildirim, Akif Koray Güney, Irfan Erol, Belma Durupinar
In the present study, 175 coagulase-positive Staphylococcus (CPS) isolates recovered from samples of beef (n = 110), raw milk n = 56), and fish (n = 9) were analyzed for methicillin resistance using MIC and PCR assays. Methicillin-resistant (MR) Staphylococcus aureus (SA) isolates were then characterized using pulsed-field gel electrophoresis (PFGE). According to findings, 62 (35.4%) of the isolates (44 from beef, 9 from milk, and 9 from fish) were identified as S. aureus based on the presence of the nuc gene...
November 2016: Journal of Food Protection
https://www.readbyqxmd.com/read/28179118/genetic-diversity-of-methicillin-resistant-staphylococcus-aureus-strains-isolated-from-burn-patients-in-iran-st239-sccmec-iii-t037-emerges-as-the-major-clone
#2
Mehdi Goudarzi, Mahnaz Bahramian, Mahboobeh Satarzadeh Tabrizi, Edet E Udo, Agnes Marie Sá Figueiredo, Maryam Fazeli, Hossein Goudarzi
Methicillin-resistant Staphylococcus aureus (MRSA) as a major cause of infection in health care, hospital and community settings is a global health concern. The purpose of this study was to determine the antibiotic susceptibility pattern and distribution of circulating molecular types of MRSA in a burn hospital in Tehran, the capital of Iran. During a 10-month study period, 106 Staphylococcus aureus isolates were assessed. Isolates were subjected to susceptibility testing using the disk diffusion method and Polymerase Chain Reaction (PCR) for detection of mecA, fem and nuc genes...
February 5, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28143440/emergence-and-spread-of-a-new-community-genotype-methicillin-resistant-staphylococcus-aureus-clone-in-colombia
#3
Javier Escobar-Perez, Niradiz Reyes, Ricaurte Alejandro Marquez-Ortiz, Juan Rebollo, Hernando Pinzón, Catalina Tovar, Jaime Moreno-Castañeda, Zayda Lorena Corredor, Betsy Esperanza Castro, Maria Victoria Moncada, Natasha Vanegas
BACKGROUND: Community-genotype methicillin-resistant Staphylococcus aureus (CG-MRSA) clones are a global concern due to their resistance and increased virulence and their ability to cause infections both hospitalized patients and healthy people in the community. Here, we characterize 32 isolates of a new CG-MRSA clone. These isolates were identified in four cities in Colombia, South America. METHODS: The isolates were recovered from four different epidemiological and prospective studies that were conducted in several regions of Colombia...
January 31, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28120911/a-potential-role-of-transposon-is431-in-the-loss-of-meca-gene
#4
Aihua Wang, Kai Zhou, Yang Liu, Liang Yang, Qin Zhang, Jing Guan, Nanshan Zhong, Chao Zhuo
Acquisition of a vancomycin-resistance-determinant may trigger deletion of the mecA gene. However, the molecular mechanisms involved remain largely unknown. In this study, we successfully produced vancomycin-intermediate-resistant Staphylococcus aureus (VISA) from Methicillin-resistant-S. aureus (MRSA) through serial passages with vancomycin. Five MRSA isolates achieved a vancomycin MIC of >8 mg/ml after 45-day serial exposure to vancomycin. After 20-day passages in media without antibiotics, three of the isolates were restored to pre-induction levels, whilst the remaining 2 (3503-1 and 4126-1) retained a vancomycin MIC >6 mg/ml...
January 25, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28116552/characterisation-of-staphylococcus-aureus-isolates-from-bloodstream-infections-democratic-republic-of-the-congo
#5
S Vandendriessche, H De Boeck, A Deplano, M-F Phoba, O Lunguya, D Falay, N Dauly, J Verhaegen, O Denis, J Jacobs
Staphylococcus aureus is known worldwide as an invasive pathogen, but information on S. aureus from bloodstream infections in Central Africa remains scarce. A collection of S. aureus blood culture isolates recovered from hospitals in four provinces in the Democratic Republic of the Congo (2009-2013) was assessed. A total of 27/108 isolates were methicillin-resistant S. aureus (MRSA), of which >70% were co-resistant to aminoglycosides, tetracyclines, macrolides and lincosamides. For MRSA and methicillin-susceptible S...
January 23, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28109847/high-frequency-of-sccmec-type-v-and-agr-type-i-among-heterogeneous-vancomycin-intermediate-staphylococcus-aureus-hvisa-in-north-india
#6
Avinash Singh, Kashi Nath Prasad, Mohibur Rahman, Ravi Prakash Rai, Satyendra Kumar Singh, Janmejai Kumar Srivastava
OBJECTIVES: The objective of this study was to compare the genetic features of heterogeneous vancomycin-intermediate Staphylococcus aureus (hVISA) and vancomycin-sensitive methicillin-resistant S. aureus (VS-MRSA) isolates. METHODS: The presence of staphylococcal cassette chromosome mec (SCCmec) types, Panton-Valentine leukocidin (PVL), accessory gene regulator (agr) types, and vanA and vanB genes in hVISA and VS-MRSA isolates was evaluated by PCR. Genetic relatedness was studied by pulsed-field gel electrophoresis (PFGE)...
January 18, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28108681/re-emergence-of-methicillin-susceptibility-in-a-resistant-lineage-of-staphylococcus-aureus
#7
Alice Ledda, James R Price, Kevin Cole, Martin J Llewelyn, Angela M Kearns, Derrick W Crook, John Paul, Xavier Didelot
OBJECTIVES: MRSA is a leading cause of hospital-associated infection. Acquired resistance is encoded by the mecA gene or its homologue mecC, but little is known about the evolutionary dynamics involved in gain and loss of resistance. The objective of this study was to obtain an expanded understanding of Staphylococcus aureus methicillin resistance microevolution in vivo, by focusing on a single lineage. METHODS: We compared the whole-genome sequences of 231 isolates from a single epidemic lineage [clonal complex 30 (CC30) and spa-type t018] of S...
January 20, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28096484/evolutionary-dynamics-of-pandemic-methicillin-sensitive-staphylococcus-aureus-st398-and-its-international-spread-via-routes-of-human-migration
#8
Anne-Catrin Uhlemann, Paul R McAdam, Sean B Sullivan, Justin R Knox, Hossein Khiabanian, Raul Rabadan, Peter R Davies, J Ross Fitzgerald, Franklin D Lowy
: Methicillin-susceptible Staphylococcus aureus (MSSA) accounts for the majority of S. aureus infections globally, and yet surprisingly little is known about its clonal evolution. We applied comparative whole-genome sequencing (WGS) analyses to epidemiologically and geographically diverse ST398-MSSA, a pandemic lineage affecting both humans and livestock. Bayesian phylogenetic analysis predicted divergence of human-associated ST398-MSSA ~40 years ago. Isolates from Midwestern pigs and veterinarians differed substantially from those in New York City (NYC)...
January 17, 2017: MBio
https://www.readbyqxmd.com/read/28069659/mrsa-strains-carrying-the-novel-mecc-gene-full-genome-sequencing-identifies-in-the-genetic-background-several-determinants-that-modulate-the-resistant-phenotype
#9
Catarina Milheiriço, Hermínia de Lencastre, Alexander Tomasz
Most MRSA strains are resistant to beta-lactam antibiotics due to the presence of the mecA gene encoding an extra penicillin-binding protein (PBP2A) that has low affinity to virtually all beta-lactam antibiotics. Recently, a new resistance determinant - the mecC gene - was identified in S. aureus isolates recovered from humans and dairy cattle. Although having typically low MICs to beta-lactam antibiotics, MRSA strains with the mecC determinant are also capable of expressing high levels of oxacillin resistance when in an optimal genetic background...
January 9, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28004519/antimicrobial-assay-and-genetic-screening-of-selected-freshwater-cyanobacteria-and-identification-of-a-biomolecule-dihydro-2h-pyran-2-one-derivative
#10
A Srivastava, V K Singh, S Patnaik, J Tripathi, P Singh, G Nath, R K Asthana
AIMS: Explorations of freshwater cyanobacteria as antimicrobial (bacteria, fungi and MRSA strains) drug resource using bioassay, NRPS (non-ribosomal polypeptide synthetase) and PKS (polyketide synthase) genes, as well as in silico approach. METHODS AND RESULTS: We have bioassayed the extracts of Phormidium CCC727, Geitlerinema CCC728, Arthrospira CCC729, Leptolyngbya CCC732, Phormidium CCC730, Phormidium CCC731 against six pathogenic bacteria Gram (+ve): Staphylococcus aureus including seven clinical methicillin-resistant S...
December 22, 2016: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28003353/this-article-has-been-temporarily-withdrawn-and-will-be-reposted-within-a-supplement-in-2017-the-press-apologizes-for-any-inconvenience-caused
#11
EDITORIAL
(no author information available yet)
No abstract text is available yet for this article.
December 21, 2016: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/27999423/characterization-of-community-associated-staphylococcus-aureus-from-skin-and-soft-tissue-infections-a-multicenter-study-in-china
#12
MULTICENTER STUDY
Ying Liu, Zhe Xu, Zhou Yang, Juan Sun, Lin Ma
We evaluated the epidemiological and molecular features of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) and methicillin-sensitive S. aureus (MSSA) from children and adult patients with skin and soft-tissue infections (SSTIs) in China. Prospective community-acquired S. aureus SSTI surveillance was conducted in 23 hospitals over a 24-month period. Susceptibility to 16 antimicrobials was evaluated using the agar dilution method. StatApriori was used to determine statistically significant association trends...
December 21, 2016: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/27990009/distribution-of-methicillin-resistant-coagulase-positive-staphylococci-mrcops-in-a-surgical-unit-and-cystotomy-operation-sites-in-a-veterinary-teaching-hospital
#13
Fungwithaya Punpichaya, Brikshavana Pasakorn, Chanchaithong Pattrarat, Prapasarakul Nuvee
This study aimed to investigate the spread of methicillin-resistant coagulase-positive staphylococci (MRCoPS) among veterinary staff, hand-touch sites and surgical tissue during cystotomy operations on cats and dogs that were patients, and to analyze the genetic relatedness and antimicrobial resistance profiles of the isolates. Human and environmental samples were obtained from the nasal passageways of 12 surgeons and veterinary assistants and from 29 hand-touch sites of instruments in operative units and subjected to bacterial isolation and enumeration...
December 17, 2016: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/27955932/molecular-analysis-of-meticillin-resistant-staphylococcus-aureus-strains-isolated-from-different-types-of-infections-from-patients-hospitalized-in-12-regional-non-teaching-hospitals-in-southern-poland
#14
A Chmielarczyk, M Pomorska-Wesołowska, A Szczypta, D Romaniszyn, M Pobiega, J Wójkowska-Mach
BACKGROUND: Accumulated data indicate that meticillin-resistant Staphylococcus aureus (MRSA) infections are associated with a worse prognosis than methicillin-susceptible S. aureus infections. AIM: To assess the epidemiological profile of S. aureus infections and the genetic diversity of clinical strains of MRSA in 12 hospitals in southern Poland. METHODS: Samples from bloodstream infections, pneumonia, and skin and soft tissue infections from adult patients were examined...
March 2017: Journal of Hospital Infection
https://www.readbyqxmd.com/read/27936152/variety-of-antimicrobial-resistances-and-virulence-factors-in-staphylococcus-aureus-isolates-from-meat-products-legally-and-illegally-introduced-to-germany
#15
Anja Müller, Diana Seinige, Wiebke Jansen, Günter Klein, Ralf Ehricht, Stefan Monecke, Corinna Kehrenberg
Food products of animal origin can serve as a vehicle for Staphylococcus (S.) aureus, a facultative pathogen involved in a variety of diseases. As a result, international trade and illegal transportation of foodstuffs can facilitate the distribution of S. aureus over long distances. In this study, we investigated S. aureus isolates recovered from meat products confiscated from passengers returning from non-EU countries at two German airports and from samples of legally imported meats from non-EU countries. The aim was to characterize isolates in regard to their genetic relatedness as well as their antimicrobial resistance profiles and major virulence factors in order to assess potential risks associated with these products...
2016: PloS One
https://www.readbyqxmd.com/read/27902380/antimicrobial-resistance-profiling-and-molecular-typing-of-methicillin-resistant-staphylococcus-aureus-isolated-from-a-malaysian-teaching-hospital
#16
Ainihayati Noordin, Hassriana Fazzila Sapri, Nurul Azirah Mohamad Sani, Shih Kei Leong, Xin-Ee Tan, Toh Leong Tan, Noraziah Mohamad Zin, Hui-Min Neoh, Salasawati Hussin
The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30 % to 40 % of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of our teaching hospital located in Kuala Lumpur, the capital city of Malaysia, and performed antimicrobial susceptibility testing on these strains. The strains were then molecularly characterized via staphylococcal cassette chromosome (SCC) mec and virulence gene (cna, sea, seb, sec, sed, see, seg, seh, sei, eta, etb, Panton-Valentine leukocidin and toxic shock syndrome toxin-1) typing; a subset of 49 strains isolated from the intensive care unit was also typed using PFGE...
December 2016: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/27899074/antibiotic-resistance-and-clonal-diversity-of-invasive-staphylococcus-aureus-in-the-rural-ashanti-region-ghana
#17
Denise Dekker, Manuel Wolters, Eva Mertens, Kennedy Gyau Boahen, Ralf Krumkamp, Daniel Eibach, Norbert G Schwarz, Yaw Adu-Sarkodie, Holger Rohde, Martin Christner, Florian Marks, Nimako Sarpong, Jürgen May
BACKGROUND: Staphylococcus aureus is among the most common pathogens isolated from blood cultures in Ghana; yet the epidemiology of blood infections in rural settings is poorly described. This study aims to investigate antimicrobial susceptibility and clonal diversity of S. aureus causing bloodstream infections in two hospitals in the Ashanti Region, Ghana. METHODS: Blood cultures were performed for all febrile patients (≥37.5 °C) on hospital admission. Antibiotic susceptibility testing for S...
November 29, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27895635/molecular-characteristic-and-virulence-gene-profiles-of-community-associated-methicillin-resistant-staphylococcus-aureus-isolates-from-pediatric-patients-in-shanghai-china
#18
Xing Wang, Xia Li, Wei Liu, Weichun Huang, Qihua Fu, Min Li
Staphylococcus aureus is a globally important human pathogen, especially among children and immunocompromised patients. The emergence and spread of community-associated methicillin-resistant S. aureus (CA-MRSA) has become a serious public health problem worldwide. The aim of this study was to investigate the prevalence, molecular characteristics and virulence profiles of CA-MRSA infections from pediatric patients in a university hospital in Shanghai, China. A total of 80 CA-MRSA isolates were collected from July 2012 to December 2013 in Shanghai Children's Medical Center and analyzed by multilocus sequence typing, staphylococcus chromosomal cassette mec (SCCmec) typing, and spa typing...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27881973/livestock-associated-methicillin-resistant-staphylococcus-aureus-la-mrsa-clonal-complex-cc-398-isolated-from-uk-animals-belong-to-european-lineages
#19
Meenaxi Sharma, Javier Nunez-Garcia, Angela M Kearns, Michel Doumith, Patrick R Butaye, M Angeles Argudín, Angela Lahuerta-Marin, Bruno Pichon, Manal AbuOun, Jon Rogers, Richard J Ellis, Christopher Teale, Muna F Anjum
In recent years, there has been an increase in the number of livestock-associated methicillin resistant Staphylococcus aureus (LA-MRSA) clonal complex (CC) 398 recovered from S. aureus isolated animals in the UK. To determine possible origins of 12 LA-MRSA CC398 isolates collected after screening more than a thousand S. aureus animal isolates from the UK between 2013 and 2015, whole genome sequences (WGS) of CC398 European, including UK, and non-European isolates from diverse animal hosts were compared. Phylogenetic reconstruction applied to WGS data to assess genetic relatedness of all 89 isolates, clustered the 12 UK CC398 LA-MRSA within the European sub-lineages, although on different nodes; implicating multiple independent incursions into the UK, as opposed to a single introduction followed by clonal expansion...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27869532/molecular-characterization-of-methicillin-resistant-staphylococcus-aureus-from-outpatients-in-northern-japan-increasing-tendency-of-st5-st764-mrsa-iia-with-arginine-catabolic-mobile-element
#20
Meiji Soe Aung, Mitsuyo Kawaguchiya, Noriko Urushibara, Ayako Sumi, Masahiko Ito, Kenji Kudo, Shigeo Morimoto, Shino Hosoya, Nobumichi Kobayashi
Arginine catabolic mobile element (ACME) is a genomic island of staphylococcus and is considered to confer enhanced ability to survive and growth on host bacterial cells. ACME has been typically identified in Panton-Valentine Leukocidin (PVL)-positive ST8 methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type IVa (USA300 clone), and it is also found in other lineages at low frequency. Prevalence and molecular characteristics of PVL(+)and/or ACME(+) MRSA were investigated for 624 clinical isolates collected from outpatients in northern Japan from 2013 to 2014...
November 21, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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