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https://www.readbyqxmd.com/read/29760649/brain-region-dependent-rejection-of-neural-precursor-cell-transplants
#1
Nina Fainstein, Tamir Ben-Hur
The concept of CNS as an immune-privileged site has been challenged by the occurrence of immune surveillance and allogeneic graft rejection in the brain. Here we examined whether the immune response to allogeneic neural grafts is determined by the site of implantation in the CNS. Dramatic regional differences were observed between immune responses to allogeneic neural precursor/stem cell (NPC) grafts in the striatum vs. the hippocampus. Striatal grafts were heavily infiltrated with IBA-1+ microglia/macrophages and CD3+ T cells and completely rejected...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29740779/forced-expression-of-cd200-improves-the-differentiation-capability-and-immunoregulatory-functions-of-mesenchymal-stromal-cells
#2
Hye Joung Kim, Kyoung-Woon Kim, Yong-Rim Kwon, Bo-Mi Kim, Yoo-Jin Kim
OBJECTIVE: In order to identify specific mesenchymal stromal cell (MSC) populations with enhanced therapeutic efficacy, we evaluated the functional changes associated with the stable expression of CD200, which is associated with immune regulatory function and osteogenic differentiation, in human bone marrow-derived MSCs (CD200/MSCs). RESULTS: We detected significantly greater osteogenesis and chondrogenesis in CD200/MSCs than in mock-transfected MSCs. In addition, the immune regulatory function of MSCs in mixed lymphocyte reactions was enhanced by CD200 gene transfection...
May 9, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29729356/loss-of-neuronal-cd200-contributed-to-microglial-activation-after-acute-cerebral-ischemia-in-mice
#3
Yang Yang, Xiang-Jian Zhang, Cong Zhang, Rong Chen, Li Li, Junna He, Yanzhao Xie, Yanxia Chen
CD200 has been proved to play a role in immuno-inflammatory reaction. However, little information is available on CD200 in the acute stage of cerebral ischemia. We investigated the association between neuronal death and expression of CD200, and explored the relationship between CD200 and microglia in cerebral ischemic mice. Firstly, localization of CD200 expression in the normal brain tissue was detected by immunofluorescent assay. Then, focal cerebral ischemia was induced in mice by permanent middle cerebral artery occlusion (pMCAO) and then cortical tissues were collected at 6, 12, 24 and 48 h after surgery...
May 2, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29729150/cd200-cx3cl1-and-trem2-mediated-neuron-microglia-interactions-and-their-involvements-in-alzheimer-s-disease
#4
Lihang Zhang, Juan Xu, Jinchao Gao, Yuncheng Wu, Ming Yin, Wenjuan Zhao
Neurons and microglia are two major components in the central nervous system (CNS). The interactions between them play important roles in maintaining homeostasis of the brain. In recent years, substantial studies have focused on the interactions between neurons and microglia, revealing that microglia become reactive when the interactions are pathophysiologically interfered, usually accompanying neuronal injury, which is a common feature for Alzheimer's disease (AD). Many molecules and factors participate in these physiological and pathological processes, either in a contact-dependent or a contact-independent manner...
May 5, 2018: Reviews in the Neurosciences
https://www.readbyqxmd.com/read/29721394/merkel-cell-carcinoma-expresses-the-immunoregulatory-ligand-cd200-and-induces-immunosuppressive-macrophages-and-regulatory-t-cells
#5
Maria Rita Gaiser, Cleo-Aron Weis, Timo Gaiser, Hong Jiang, Kristina Buder-Bakhaya, Esther Herpel, Arne Warth, Ying Xiao, Lingling Miao, Isaac Brownell
Merkel cell carcinoma (MCC) is a rare and aggressive skin cancer that responds to PD-1/PD-L1 immune checkpoint inhibitors. CD200 is another checkpoint modulator whose receptor is found on tumor-promoting myeloid cells, including M2 macrophages. We found high CD200 mRNA expression in MCC tumors, and CD200 immunostaining was demonstrated on 95.5% of MCC tumors. CD200R-expressing myeloid cells were present in the MCC tumor microenvironment. MCC-associated macrophages had a higher average CD163:CD68 staining ratio (2...
2018: Oncoimmunology
https://www.readbyqxmd.com/read/29721190/cd200fc-enhances-anti-tumoral-immune-response-and-inhibits-visceral-metastasis-of-breast-carcinoma
#6
Nuray Erin, Gamze Tanrıöver, Anna Curry, Muhlis Akman, Özlem Duymuş, Reg Gorczynski
CD200 is a widely expressed cell surface glycoprotein that inhibits excessive inflammation in autoimmunity, transplantation, and viral infections. We previously observed that visceral metastasis of highly aggressive and inflammatory 4THM breast carcinoma cells was markedly decreased in CD200 transgenic mice. The goal of this study was to determine whether exogenous exposure to CD200fc mimics the effects of endogenously over expressed CD200. Female BALB/c mice were injected with CD200fc two times a week for five times...
April 10, 2018: Oncotarget
https://www.readbyqxmd.com/read/29720577/universal-monitoring-of-minimal-residual-disease-in-acute-myeloid-leukemia
#7
Elaine Coustan-Smith, Guangchun Song, Sheila Shurtleff, Allen Eng-Juh Yeoh, Wee Joo Chng, Siew Peng Chen, Jeffrey E Rubnitz, Ching-Hon Pui, James R Downing, Dario Campana
BACKGROUND: Optimal management of acute myeloid leukemia (AML) requires monitoring of treatment response, but minimal residual disease (MRD) may escape detection. We sought to identify distinctive features of AML cells for universal MRD monitoring. METHODS: We compared genome-wide gene expression of AML cells from 157 patients with that of normal myeloblasts. Markers encoded by aberrantly expressed genes, including some previously associated with leukemia stem cells, were studied by flow cytometry in 240 patients with AML and in nonleukemic myeloblasts from 63 bone marrow samples...
May 3, 2018: JCI Insight
https://www.readbyqxmd.com/read/29715095/the-immune-checkpoint-molecule-cd200-is-associated-with-tumor-grading-and-metastasis-in-bladder-cancer
#8
Peter Rexin, Alessa Tauchert, Jörg Hänze, Hendrik Heers, Ansgar Schmidt, Rainer Hofmann, Axel Hegele
BACKGROUND: We examined the expression of CD200, a ligand of immune tolerance, in transitional cell carcinoma of the human bladder (TCC). MATERIALS AND METHODS: CD200 was analyzed by immunohistochemistry (IHC) in 90 patients with suspected TCC lesions of the bladder. Expression of CD200 was exemplarily validated by quantitative reverse transcription polymerase chain reaction and western blot analysis. RESULTS: CD200 was detectable at mRNA and protein levels in TCC homogenate and TCC cell lines (T24, UMUC3)...
May 2018: Anticancer Research
https://www.readbyqxmd.com/read/29702149/the-link-between-bone-microenvironment-and-immune-cells-in-multiple-myeloma-emerging-role-of-cd38
#9
REVIEW
Marina Bolzoni, Denise Toscani, Federica Costa, Emanuela Vicario, Franco Aversa, Nicola Giuliani
The relationship between bone and immune cells is well established both in physiological and pathological conditions. Multiple myeloma (MM) is a plasma cell malignancy characterized by an increase of number and activity of osteoclasts (OCLs) and a decrease of osteoblasts (OBs). These events are responsible for bone lesions of MM patients. OCLs support MM cells survival in vitro and in vivo. Recently, the possible role of OCLs as immunosuppressive cells in the MM BM microenvironment has been underlined. OCLs protect MM cells against T cell-mediated cytotoxicity through the expression of several molecules including programmed death-ligand (PD-L) 1, galectin (Gal) 9, CD200, and indoleamine-2,3-dioxygenase (IDO)...
April 24, 2018: Immunology Letters
https://www.readbyqxmd.com/read/29698858/cell-membrane-bound-cd200-signals-both-via-an-extracellular-domain-and-following-nuclear-translocation-of-a-cytoplasmic-fragment
#10
Zhiqi Chen, Andras Kapus, Ismat Khatri, Olha Kos, Fang Zhu, Reginald M Gorczynski
In previous studies we had reported that the immunosuppressive cell membrane bound molecule CD200 is released from the cell following cleavage by matrix metalloproteases, with the released soluble CD200 acting as an immunosuppressant following binding to, and signaling through, its cognate receptor CD200R expressed on target cells. We now show that although the intracellular cytoplasmic tail (CD200C-tail ) of CD200 has no consensus sites for adapter molecules which might signal the CD200+ cell directly, cleavage of the CD200C-tail from the membrane region of CD200 by a consensus γ-secretase, leads to nuclear translocation and DNA binding (identified by chromatin immunoprecipitation followed by sequencing, Chip-sequencing) of the CD200C-tail ...
April 19, 2018: Leukemia Research
https://www.readbyqxmd.com/read/29675782/identification-of-human-cutaneous-basal-cell-carcinoma-cancer-stem-cells
#11
Huw Morgan, Carlotta Olivero, Girish K Patel
The cancer stem cell model states that a subset of tumor cells, called "cancer stem cells," can initiate and propagate tumor growth through self-renewal, high proliferative capacity, and their ability to recreate tumor heterogeneity. In basal cell carcinoma (BCC), we have shown that tumor cells that express the cell surface protein CD200 fulfill the cancer stem cell hypothesis. CD200+ CD45- BCC cells represent 0.05-3.96% of all BCC cells and reside in small clusters at the tumor periphery. Using a novel, reproducible in vivo xenograft growth assay, we determined that tumor-initiating cell (TIC) frequencies are approximately 1 per 1...
April 20, 2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29602219/cd200-included-in-a-4-marker-modified-matutes-score-provides-optimal-sensitivity-and-specificity-for-the-diagnosis-of-chronic-lymphocytic-leukaemia
#12
Giovanni D'Arena, Candida Vitale, Giovanni Rossi, Marta Coscia, Paola Omedè, Fiorella D'Auria, Teodora Statuto, Luciana Valvano, Stefania Ciolli, Milena Gilestro, Stefano Molica, Silvia Bellesi, Topini Giuseppe, Panichi Valentina, Francesco Autore, Idanna Innocenti, Pellegrino Musto, Silvia Deaglio, Luca Laurenti, Luigi Del Vecchio
CD200, a transmembrane type Ia glycoprotein belonging to the immunoglobulin superfamily, has been shown to have a differential expression in B-cell neoplasms. Here, we retrospectively assessed the diagnostic relevance of CD200 on 427 patients with B-cell chronic neoplasms in leukemic phase (median age, 69 y; range, 35-97 y). The final diagnosis based on the investigator's assessment was chronic lymphocytic leukaemia (CLL) in 75% of cases and non-CLL in 25% of cases. Sensitivity and specificity for the diagnosis of CLL (vs non-CLL) were calculated for the following markers: CD200, CD5, CD22, CD23, CD79b, FMC7, and SmIg...
March 30, 2018: Hematological Oncology
https://www.readbyqxmd.com/read/29571945/trophoblast-cd200-expression-in-successful-human-pregancies-and-missed-abortions
#13
David A Clark, M Elizabeth McCready, Kate Harris, Leslie Malloy, Jorge L Arredondo
Presence of the CD200 immune check-point inhibitor at the feto-maternal interface is linked to prevention of spontaneous abortion in mice and humans. In human missed abortions (MA), absence of Th17-driven inflammation has been attributed to expression of villus trophoblast CD200 quantified using immunohistochemistry. While rapid aneuploidy (QF-PCR) testing linked low CD200 to pregnancy failure, data showing normal VT CD200 in first trimester normal pregnancy and in abortion of chromosomally abnormal embryos has not been demonstrated...
March 16, 2018: Journal of Reproductive Immunology
https://www.readbyqxmd.com/read/29567884/utility-of-cd200-expression-and-cd20-antibody-binding-capacity-in-differentiating-chronic-lymphocytic-leukemia-from-other-chronic-lymphoproliferative-disorders
#14
R Poongodi, Neelam Varma, Shano Naseem, Bose Parveen, Subhash Varma
Background: Chronic lymphoproliferative disorders (CLPDs) are heterogeneous group of disorders with variable clinical presentations and outcomes. Therefore, accurate classification is crucial for treatment planning. At present, flow cytometry immunophenotyping (FCM-IPT) is a useful tool for diagnosing these diseases. However, overlapping immunophenotypes do exist. Recently, differential expression of CD200 and variation in number of CD20 antibody bound per cell (ABC) in different CLPDs has been reported...
January 2018: Indian Journal of Pathology & Microbiology
https://www.readbyqxmd.com/read/29532324/characterization-of-an-in-vitro-model-system-to-explore-control-of-tumor-invasion-of-emt6-and-4thm-breast-tumors-by-cd200-cd200r-interactions
#15
Reginald M Gorczynski, Nuray Erin, Tahir Maqbool, Christopher P Gorczynski, Laura Y Gorczynski
BACKGROUND AND PURPOSE: In BALB/c mice with transplantable breast tumors, we showed that CD200R1KO mice were cured of local and metastatic growth of EMT6 cells following surgical resection of localized tumor and immunization with irradiated cells along with CpG as adjuvant. On the other hand, wild-type (WT) animals treated in the same fashion develop pulmonary and liver metastases within 20 days of surgery. To develop an in vitro system which would mimic the in vivo model and allow exploration of factors controlling tumor invasion as a precursor to in vivo metastasis, we have developed and characterized a two-phase culture system...
March 12, 2018: Breast Cancer: the Journal of the Japanese Breast Cancer Society
https://www.readbyqxmd.com/read/29525600/an-autologous-tumor-vaccine-for-cll
#16
Fang Zhu, Ismat Khatri, David Spaner, Reginald M Gorczynski
Chronic Lymphocytic Leukemia B cells (CLL) are malignant cells which retain at least some functions of normal B cells. Paramount amongst the latter is that when such cells are appropriately stimulated, they are able to present antigens, including any potential tumor antigens, making them excellent choices as a candidate tumor vaccine. We show that following stimulation of CLL cells with Phorbol myristic acetate, IL-2, the TLR7 agonist imiquimod (P2I) and ionomycin (P2Iio), markedly increased expression of CD54 and CD83 was seen, indicative of B cell activation and a transition to antigen-presenting cells...
May 2018: Leukemia Research
https://www.readbyqxmd.com/read/29498809/antibody-based-cell-surface-proteome-profiling-of-metastatic-breast-cancer-primary-explants-and-cell-lines
#17
Vera S Donnenberg, Jayce Jieming Zhang, Erika Moravcikova, Ernest Michael Meyer, Haihui Lu, Christian T Carson, Albert D Donnenberg
Flow cytometric cell surface proteomics provides a new and powerful tool to determine changes accompanying neoplastic transformation and invasion, providing clues to essential interactions with the microenvironment as well as leads for potential therapeutic targets. One of the most important advantages of flow cytometric cell surface proteomics is that it can be performed on living cells that can be sorted for further characterization and functional studies. Here, we document the surface proteome of clonogenic metastatic breast cancer (MBrCa) explants, which was strikingly similar to that of normal mesenchymal stromal cells (P = 0...
April 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
https://www.readbyqxmd.com/read/29429517/in-vitro-identification-of-a-stem-cell-population-from-canine-hair-follicle-bulge-region
#18
Raquel V G de Castro, Mariana R Tavares, Fabiana F Bressan, Naira C G Pieri, Amanda Baracho Trindade Hill, Aline F Souza, Nathan da R N Cruz, Daniele S Martins, Carlos E Ambrósio, Flávio V Meirelles, Joaquim M Garcia
Skin is an extensive and easily accessible organ possessing various cell types that are constantly renewed. Previous studies have suggested the presence of a stem cell niche at the bulge region of the hair follicle, which contains cells positive for CD200 and CD34. Thus, this study sought to identify these cell populations in canine skin cells using the following methods 1- collecting samples of adult and fetal skin and isolating and culturing these cells using a method of simple enzymatic digestion and 2- testing the cell cultures for CD200 and CD34 in vitro and comparing them with skin tissue samples (in situ)...
February 2018: Tissue & Cell
https://www.readbyqxmd.com/read/29407579/skin-and-scale-regeneration-after-mechanical-damage-in-a-teleost
#19
Rita A Costa, Deborah M Power
Skin wound healing has been widely studied in mammalian models but the information on teleost cutaneous healing is sparse and frequently considered in the context of viral or bacterial infections or parasitic infestations in aquaculture. In the present study a detailed time course (0 h, 6 h, 1, 2, 3 and 4 days) coupled to morphology and gene expression analysis revealed rapid regeneration of skin without scarring in a marine teleost after a superficial wound caused by the loss of a large area of scales...
March 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29347962/primary-explant-culture-and-collagen-i-substrate-enhances-corneal-endothelial-cell-morphology
#20
Judith Zavala, María-Dolores Montalvo-Parra, Guillermo-Isaac Guerrero-Ramírez, Carlos-Alberto Rodríguez-Barrientos, Victor Treviño, Jorge E Valdez-García
OBJECTIVES: Corneal endothelial cell (CEC) isolation and harvest aim to produce engineered grafts to solve donor corneal tissue shortage. To yield high amounts of CEC maintaining morphological and molecular characteristics, several isolation and culture conditions are reported. Here, we combined direct explant culture, with three different coating conditions and a two-step media approach to compare confluence efficiency, morphology, and specific molecular markers expression. DATA DESCRIPTION: Confluence was reached after 2 weeks in the three coating conditions (Matrigel, collagen I, and in uncoated plates) using a two-step approach (proliferative medium without pituitary extract, followed by stabilizer basal medium)...
January 18, 2018: BMC Research Notes
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