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https://www.readbyqxmd.com/read/28937880/detection-of-free-microcystins-in-the-liver-and-muscle-of-freshwater-fish-by-liquid-chromatography-tandem-mass-spectrometry
#1
Xiaobin Hu, Jinyun Ye, Rongfei Zhang, Xiang Wu, Yixiang Zhang, Chenglong Wu
MC analysis of biological tissue is considered to be very difficult due to the lack of validated methods. This is the primary limiting factor for monitoring potential risks in both the flesh of aquatic organisms and the aquatic ecosystem. In this study, an effective method to determine free MCs (MC-LR and MC-RR) in the muscle and liver tissues of freshwater cultured fish was developed using solid-phase extraction (SPE) and liquid chromatography-tandem mass spectrometry (LC/MS-MS). The extraction solvent, time of extraction, eluent and purification of the extract were optimized...
September 22, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28937345/practical-implications-of-the-minimally-conscious-state-diagnosis-in-adults
#2
Karola V Kreitmair, Katherine E Kruse
This article addresses questions surrounding the minimally conscious state (MCS) from the perspective of adult clinical ethics. It describes the background of the MCS diagnosis, analyzes phenomenological ambiguities inherent in the nature of MCS, and raises epistemological concerns surrounding its diagnosis. It argues that in many cases, the burdens of prolonging treatment for people who have sustained certain severe brain injuries (SBI) outweigh the benefits, even if they are in or have the prospect of entering into MCS...
October 2017: Cambridge Quarterly of Healthcare Ethics: CQ: the International Journal of Healthcare Ethics Committees
https://www.readbyqxmd.com/read/28934906/autonomic-symptoms-in-patients-with-moderate-and-severe-chronic-obstructive-pulmonary-disease
#3
Jibril Mohammed, Eric Derom, Inge De Wandele, Lies Rambaut, Patrick Calders
OBJECTIVES: A synoptic description of the autonomic symptoms profile (ASP) of patients with COPD is not available. Therefore, we aimed to provide an overview of autonomic symptoms and its associates in COPD. METHODS: We evaluated 89 subjects with COPD (65 ± 7.3 years; 66 males; GOLD II-IV) with an equal number of age- and sex-matched control subjects by means of the composite autonomic symptom score (COMPASS 31) questionnaire, which assesses autonomic symptoms across six domains (orthostatic intolerance, vasomotor, secretomotor, gastrointestinal, urinary and pupillomotor)...
September 22, 2017: Acta Clinica Belgica
https://www.readbyqxmd.com/read/28932032/mast-cells-and-angiogenesis-in-malignant-and-premalignant-oral-lesions-an-immunohistochemical-study
#4
Daisy Laishram, Kavita Rao, H S Uma Devi, N S Priya, T Smitha, H S Sheethal
INTRODUCTION AND OBJECTIVES: Angiogenesis is a complex event facilitated by angiogenic factors released from neoplastic and host immune cells. Among host immune cells, mast cells (MCs) may have greater significance in tumor progression through angiogenesis. The objectives of the study were to evaluate and correlate mast cell density (MCD) and microvessel density (MVD) in normal gingival tissue, leukoplakia with and without dysplasia and oral squamous cell carcinoma (OSCC) cases. MATERIALS AND METHODS: Among eighty selected cases, twenty were of normal gingiva, twenty each of leukoplakia without and with dysplasia and twenty of OSCC...
May 2017: Journal of Oral and Maxillofacial Pathology: JOMFP
https://www.readbyqxmd.com/read/28931587/maternal-choline-supplementation-modulates-placental-nutrient-transport-and-metabolism-in-late-gestation-of-mouse-pregnancy
#5
Sze Ting Cecilia Kwan, Julia H King, Jian Yan, Zhen Wang, Xinyin Jiang, Jason S Hutzler, Hallie R Klein, J Thomas Brenna, Mark S Roberson, Marie A Caudill
Background: Fetal growth is dependent on placental nutrient supply, which is influenced by placental perfusion and transporter abundance. Previous research indicates that adequate choline nutrition during pregnancy improves placental vascular development, supporting the hypothesis that choline may affect placental nutrient transport.Objective: The present study sought to determine the impact of maternal choline supplementation (MCS) on placental nutrient transporter abundance and nutrient metabolism during late gestation...
September 20, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28930844/health-related-quality-of-life-assessment-in-surgical-patients-with-papillary-thyroid-carcinoma-a-single-center-analysis-from-mainland-china
#6
Juxiang Gou, Wenjie Cheng, Jianyong Lei, Qian Pan, Wei You, Ming Cai, Huairong Tang, Yali Lei, Zhihui Li, Rixiang Gong, Jinqiang Zhu
Even with a favorable prognosis, the health-related quality of life (HRQoL) of papillary thyroid carcinoma (PTC) patients remains unclear and conflicting. Thus, in the present study, we compared the HRQoL of PTC patients with that of the general population (GP).The study was performed in our thyroid and parathyroid surgery department, and 186 PTC patients who had undergone thyroidectomy were included. The exclusion criteria were an age < 18 years, no follow-up, and the presence of other malignant neoplasms...
September 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28930809/survival-benefit-of-adjuvant-radiotherapy-an-analysis-of-low-stage-invasive-ovarian-mucinous-carcinomas
#7
Aalok Kumar, Nhu Le, C Blake Gilks, Jennifer L Santos, Frances Wong, Kenneth Swenerton, Paul J Hoskins, Jessica N McAlpine, Anna V Tinker
OBJECTIVE: Our aim was to evaluate the population-based outcomes of stages I and II invasive ovarian mucinous carcinomas (MCs) treated with adjuvant platinum-based chemotherapy and abdominopelvic radiotherapy (XRT). METHODS: International Federation of Gynecology and Obstetrics stage I/II MC cases referred to the British Columbia Cancer Agency between 1984 and 2014 were reviewed. Chemotherapy (minimum of 3 cycles) and XRT were the institutional policy for stages IA/B (grade 2/3) and IC/II (any grade)...
September 19, 2017: International Journal of Gynecological Cancer
https://www.readbyqxmd.com/read/28930432/injectable-stem-cell-laden-open-porous-microgels-that-favour-adipogenesis-in-vitro-and-in-vivo-evaluation
#8
Pengfei Xia, Kunxi Zhang, Yan Gong, Guifei Li, Shifeng Yan, Jingbo Yin
Microgels, with large surface area per volume, show great advantages in adipose tissue engineering due to their injectability and similarity with natural extracellular matrix. However, to date, no studies have tried applying microgels to adipose tissue regeneration. Herein, based on double bonded poly-(L-glutamic acid)-g-2-Hydroxyethyl methacrylate (PLGA-g-HEMA) and maleic anhydride-modified chitosan (MCS), an open porous microgel with high hydrophilicity and great injectability is successfully prepared (microgels diameter of 200-300 μm, pore diameter of 38 μm, and porosity of 88...
September 20, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28929725/-perceived-discrimination-at-work-for-being-an-immigrant-a-study-on-self-perceived-mental-health-status-among-immigrants-in-italy
#9
Anteo Di Napoli, Rosaria Gatta, Alessandra Rossi, Monica Perez, Gianfranco Costanzo, Concetta Mirisola, Alessio Petrelli
BACKGROUND: exposure to discrimination is widely understood as a social determinant of psychophysical health and a contributing factor to health inequities among social groups. Few studies exist, particularly in Italy, about the effects of discrimination among immigrants at workplace. OBJECTIVES: to analyse the association between perceived discrimination at work for being an immigrant and mental health status among immigrants in Italy. SETTING AND PARTICIPANTS: a sub-sample of 12,408 immigrants residing in Italy was analysed...
May 2017: Epidemiologia e Prevenzione
https://www.readbyqxmd.com/read/28929722/-self-perceived-health-status-among-immigrants-in-italy
#10
Alessio Petrelli, Anteo Di Napoli, Alessandra Rossi, Lidia Gargiulo, Concetta Mirisola, Gianfranco Costanzo
OBJECTIVES: to evaluate self-perceived health status of immigrants in Italy. DESIGN: cross-sectional study based on the representative national samples of the multipurpose surveys "Health conditions and use of health services" conducted in 2005 and 2013 by the Italian National Institute of Statistics (Istat). SETTING AND PARTICIPANTS: the study was conducted on the age group of 18-64: No. 80,661 in 2005, among which 3.2% were immigrants, and No...
May 2017: Epidemiologia e Prevenzione
https://www.readbyqxmd.com/read/28919690/subchronic-toxicity-of-nile-tilapia-with-different-exposure-routes-to-microcystis-aeruginosa-histopathology-liver-functions-and-oxidative-stress-biomarkers
#11
H M R Abdel-Latif, A M Abou Khashaba
BACKGROUND: Toxic cyanobacterial blooms (Microcystis aeruginosa contains microcystins [MCs]) have been reported to induce clinicopathological alterations as well as different oxidative stress in aquatic biota. AIM: Three-week subchronic exposure experiment was carried out on Nile tilapia, to determine their effects on fish behavior, tissues, liver functions, antioxidant enzymes, and lipid peroxidation. MATERIALS AND METHODS: Fish were exposed to four main treatments; orally fed diet plus toxic cells of M...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28919488/mast-cells-derived-from-human-induced-pluripotent-stem-cells-are-useful-for-allergen-tests
#12
Akira Igarashi, Yasuhiro Ebihara, Tomoaki Kumagai, Hiroyuki Hirai, Kinya Nagata, Kohichiro Tsuji
BACKGROUND: Several methods have been developed to detect allergen-specific IgE in sera. The passive IgE sensitization assay using human IgE receptor-expressing rat cell line RBL-2H3 is a powerful tool to detect biologically active allergen-specific IgE in serum samples. However, one disadvantage is that RBL-2H3 cells are vulnerable to high concentrations of human sera. Only a few human cultured cell lines are easily applicable to the passive IgE sensitization assay. However, the use of human induced pluripotent stem cells (iPSCs) to generate human mast cells (MCs) has not yet been reported...
September 14, 2017: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/28918761/quality-of-life-in-bipolar-disorder-towards-a-dynamic-understanding
#13
E Morton, G Murray, E E Michalak, R W Lam, S Beaulieu, V Sharma, P Cervantes, S V Parikh, L N Yatham
BACKGROUND: Although quality of life (QoL) is receiving increasing attention in bipolar disorder (BD) research and practice, little is known about its naturalistic trajectory. The dual aims of this study were to prospectively investigate: (a) the trajectory of QoL under guideline-driven treatment and (b) the dynamic relationship between mood symptoms and QoL. METHODS: In total, 362 patients with BD receiving guideline-driven treatment were prospectively followed at 3-month intervals for up to 5 years...
September 18, 2017: Psychological Medicine
https://www.readbyqxmd.com/read/28918680/transcranial-direct-current-stimulation-in-disorders-of-consciousness-a-review
#14
Ye Zhang, Weiqun Song
It is a challenge to evaluate and treat the patients with disorders of consciousness (DOC) in the clinic. Due to the huge costs of prolonged intensive care, the management of these patients raises great financial strain on families and important ethical questions. To date, several studies have attempted to specifically detect pharmacologic or non-pharmacologic effectiveness, until now there were no evidence-based guidelines about the treatment of patients with DOC. Recently, because of ethical and procedural limitations on the use of invasive stimulation techniques, non-invasive brain stimulation, such as the transcranial direct current stimulation (tDCS), has been investigated for improving the level of consciousness in patients with DOC...
September 18, 2017: International Journal of Neuroscience
https://www.readbyqxmd.com/read/28918246/synthesis-of-novel-magnesium-ferrite-mgfe2o4-biochar-magnetic-composites-and-its-adsorption-behavior-for-phosphate-in-aqueous-solutions
#15
Kyung-Won Jung, Soonjae Lee, Young Jae Lee
In this work, magnesium ferrite (MgFe2O4)/biochar magnetic composites (MFB-MCs) were prepared and utilized to remove phosphate from aqueous solutions. MFB-MCs were synthesized via co-precipitation of Fe and Mg ions onto a precursor, followed by pyrolysis. Characterization results confirmed that MgFe2O4 nanoparticles with a cubic spinel structure were successfully embedded in the biochar matrix, and this offered magnetic separability with superparamagnetic behavior and enabled higher phosphate adsorption performance than that of pristine biochar and sole MgFe2O4 nanoparticles...
September 8, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28913740/temporary-mechanical-circulatory-support-for-cardiogenic-shock
#16
REVIEW
Jean Touchan, Maya Guglin
Cardiogenic shock is a life-threatening presentation of severe heart failure with high morbidity and mortality. Given the modest increased in cardiac output and neutral/negative survival benefits with today's available inotropes (namely, dobutamine and milrinone), the use of mechanical circulatory support (MCS) has increased dramatically over the past 2 decades. In this review article, we discuss the physiologic concept, clinical evidence of benefit, and current use and indications/potential complications of the four most commonly used devices for MCS: intra-aortic balloon pump, Impella percutaneous ventricular assist device, TandemHeart, and extracorporeal membrane oxygenation (ECMO)...
September 14, 2017: Current Treatment Options in Cardiovascular Medicine
https://www.readbyqxmd.com/read/28913587/isotopic-effects-of-pce-induced-by-organohalide-respiring-bacteria
#17
Simon Leitner, Harald Berger, Markus Gorfer, Thomas G Reichenauer, Andrea Watzinger
Reductive dechlorination performed by organohalide-respiring bacteria (OHRB) enables the complete detoxification of certain emerging groundwater pollutants such as perchloroethene (PCE). Environmental samples from a contaminated site incubated in a lab-scale microcosm (MC) study enable documentation of such reductive dechlorination processes. As compound-specific isotope analysis is used to monitor PCE degradation processes, nucleic acid analysis-like 16S-rDNA analysis-can be used to determine the key OHRB that are present...
September 15, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28911671/albendazole-residues-in-goat-s-milk-interferences-in-microbial-inhibitor-tests-used-to-detect-antibiotics-in-milk
#18
Tamara Romero, Rafael Althaus, Vicente Javier Moya, María Del Carmen Beltrán, Wim Reybroeck, María Pilar Molina
Albendazole (ABZ) residues in goat's milk and their effect on the response of microbial inhibitor tests used for screening antibiotics were evaluated. A total of 18 Murciano-Granadina goats were treated with ABZ and individually milked once a day over a 7-day period. ABZ quantification was performed by high performance liquid chromatography. The ABZ parent drug was not detected. The maximum concentration of its metabolites (ABZ sulfoxide, ABZ sulfone, and ABZ 2-aminosulfone) was reached on the 1(st) day post treatment (260...
April 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910330/quality-of-life-measures-predict-cardiovascular-health-and-physical-performance-in-chronic-renal-failure-patients
#19
A Rogan, K McCarthy, G McGregor, T Hamborg, G Evans, S Hewins, N Aldridge, S Fletcher, N Krishnan, R Higgins, D Zehnder, S M Ting
BACKGROUND: Patients with advanced chronic kidney disease (CKD) experience complex functional and structural changes of the cardiopulmonary and musculoskeletal system. This results in reduced exercise tolerance, quality of life and ultimately premature death. We investigated the relationship between subjective measures of health related quality of life and objective, standardised functional measures for cardiovascular and pulmonary health. METHODS: Between April 2010 and January 2013, 143 CKD stage-5 or CKD5d patients (age 46...
2017: PloS One
https://www.readbyqxmd.com/read/28904962/self-rated-health-as-a-predictor-of-death-after-two-years-the-importance-of-physical-and-mental-wellbeing-postintensive-care
#20
Marie Vejen, Jakob B Bjorner, Morten H Bestle, Anne Lindhardt, Jens U Jensen
INTRODUCTION: The objective of this study is, among half-year intensive care survivors, to determine whether self-assessment of health can predict two-year mortality. METHODS: The study is a prospective cohort study based on the Procalcitonin and Survival Study trial. Half-year survivors from this 1200-patient multicenter intensive care trial were sent the SF-36 questionnaire. We used both a simple one-item question and multiple questions summarized as a Physical Component Summary (PCS) and a Mental Component Summary (MCS) score...
2017: BioMed Research International
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