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https://www.readbyqxmd.com/read/28437479/conformational-dynamics-of-bacterial-trigger-factor-in-apo-and-ribosome-bound-states
#1
Mehmet Tarik Can, Zeynep Kurkcuoglu, Gokce Ezeroglu, Arzu Uyar, Ozge Kurkcuoglu, Pemra Doruker
The chaperone trigger factor (TF) binds to the ribosome exit tunnel and helps cotranslational folding of nascent chains (NC) in bacterial cells and chloroplasts. In this study, we aim to investigate the functional dynamics of fully-atomistic apo TF and its complex with 50S. As TF accomodates a high percentage of charged residues on its surface, the effect of ionic strength on TF dynamics is assessed here by performing five independent molecular dynamics (MD) simulations (total of 1.3 micro-second duration) at 29 mM and 150 mM ionic strengths...
2017: PloS One
https://www.readbyqxmd.com/read/28434397/watching-the-availability-and-use-of-rapid-diagnostic-tests-rdts-and-artemisinin-based-combination-therapy-act
#2
Richard W Steketee, Thomas P Eisele
At the turn of this new century and after much debate, the malaria community reckoned with failing first line therapies and moved to a global recommendation for deployment of an artemisinin-based combination therapy (ACT) to treat infections due to Plasmodium falciparum. No one said it was going to be easy. This series in the Malaria Journal reports longitudinal snapshots of how the core pillar of malaria elimination of ensuring universal access to malaria diagnosis and treatment is faring-it is safe to say "not so well"...
April 24, 2017: Malaria Journal
https://www.readbyqxmd.com/read/28433962/ten-minute-snapshots-a-team-approach-to-teaching-postgraduates-about-professional-dilemmas
#3
Prabha S Chandra, G Ragesh, Santosh K Chaturvedi
As medical professionals, most of us face professional dilemmas that catch us unawares and are not discussed in medical training. One often learns about these dilemmas on one's own and deals with them with a common sense approach, rather than reflection. The professional dilemmas may concern receiving gifts from patients, handling issues of confidentiality or dealing with personal questions. There is seldom any formal instruction in competencies related to professionalism, especially in India. We adopted a team approach to provide training in these issues to postgraduate trainees in mental health and to engage them in discussion, using team feedback on snapshots of real-life professional situations, which were simulated through role play...
April 12, 2017: Indian Journal of Medical Ethics
https://www.readbyqxmd.com/read/28433629/a-novel-strategy-to-dissect-endogenous-gene-transcriptional-regulation-in-live-cells
#4
Wenqing Yang, Siliang Zhang, Yi Zhang, Xin Huang
Gene transcription is a central tenet of biology, traditionally measured by RT-PCR, microarray, or more recently, RNA sequencing. However, these measurements only provide a snapshot of the state of gene transcription and only represent an overall readout of complex transcriptional networks that regulate gene expression. In this report, we describe a novel strategy to dissect endogenous gene transcription regulation in live cells by knocking in a reporter gene, EGFP, under the control of the endogenous gene promoter, using the ARID1A gene as an example...
April 19, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28431310/characterization-of-bacterial-communities-of-donkey-milk-by-high-throughput-sequencing
#5
Maria de Los Dolores Soto Del Rio, Alessandra Dalmasso, Tiziana Civera, Maria Teresa Bottero
The interest in donkey milk (DM) is growing because of its functional properties and nutritional value, especially for children with allergies and food intolerances. However, most of the available reports of DM microbiota are based on culture-dependent methods to investigate food safety issues and the presence of lactic acid bacteria (LAB). The aim of this study was to determine the composition of DM bacterial communities using a high-throughput sequencing (HTS) approach. Bulk milk samples from Italian donkey dairy farms from two consecutive years were analysed using the MiSeq Illumina platform...
March 30, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28430432/absolute-binding-free-energies-between-t4-lysozyme-and-141-small-molecules-calculations-based-on-multiple-rigid-receptor-configurations
#6
Bing Xie, Trung Hai Nguyen, David D L Minh
We demonstrate the feasibility of estimating protein-ligand binding free energies using multiple rigid receptor configurations. Based on T4 lysozyme snapshots extracted from six alchemical binding free energy calculations with a flexible receptor, binding free energies were estimated for a total of 141 ligands. For 24 ligands, the calculations reproduced flexible-receptor estimates with a correlation coefficient of 0.90 and a root mean square error of 1.59 kcal/mol. The accuracy of calculations based on Poisson-Boltzmann/Surface Area implicit solvent was comparable to previously reported free energy calculations...
April 21, 2017: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/28430270/atomistic-non-adiabatic-dynamics-of-the-lh2-complex-with-a-gpu-accelerated-ab-initio-exciton-model
#7
Aaron Sisto, Clem Stross, Marc W van der Kamp, Michael O'Connor, Simon McIntosh-Smith, Graham T Johnson, Edward G Hohenstein, Fred R Manby, David R Glowacki, Todd J Martinez
We recently outlined an efficient multi-tiered parallel ab initio excitonic framework that utilizes time dependent density functional theory (TDDFT) to calculate ground and excited state energies and gradients of large supramolecular complexes in atomistic detail - enabling us to undertake non-adiabatic simulations which explicitly account for the coupled anharmonic vibrational motion of all the constituent atoms in a supramolecular system. Here we apply that framework to the 27 coupled bacterio-chlorophyll-a chromophores which make up the LH2 complex, using it to compute an on-the-fly nonadiabatic surface-hopping (SH) trajectory of electronically excited LH2...
April 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28424144/expertise-and-infrastructure-capacity-impacts-acute-coronary-syndrome-outcomes
#8
Carolyn M Astley, Isuru Ranasinghe, David Brieger, Chris J Ellis, Julie Redfern, Tom Briffa, Bernadette Aliprandi-Costa, Tegwen Howell, Stephen G Bloomer, Greg Gamble, Andrea Driscoll, Karice K Hyun, Chris J Hammett, Derek P Chew
Objective Effective translation of evidence to practice may depend on systems of care characteristics within the health service. The present study evaluated associations between hospital expertise and infrastructure capacity and acute coronary syndrome (ACS) care as part of the SNAPSHOT ACS registry.Methods A survey collected hospital systems and process data and our analysis developed a score to assess hospital infrastructure and expertise capacity. Patient-level data from a registry of 4387 suspected ACS patients enrolled over a 2-week period were used and associations with guideline care and in-hospital and 6-, 12- and 18-month outcomes were measured...
April 20, 2017: Australian Health Review: a Publication of the Australian Hospital Association
https://www.readbyqxmd.com/read/28422985/revising-mtdna-haplotypes-of-the-ancient-hungarian-conquerors-with-next-generation-sequencing
#9
Endre Neparáczki, Klaudia Kocsy, Gábor Endre Tóth, Zoltán Maróti, Tibor Kalmár, Péter Bihari, István Nagy, György Pálfi, Erika Molnár, István Raskó, Tibor Török
As part of the effort to create a high resolution representative sequence database of the medieval Hungarian conquerors we have resequenced the entire mtDNA genome of 24 published ancient samples with Next Generation Sequencing, whose haplotypes had been previously determined with traditional PCR based methods. We show that PCR based methods are prone to erroneous haplotype or haplogroup determination due to ambiguous sequence reads, and many of the resequenced samples had been classified inaccurately. The SNaPshot method applied with published ancient DNA authenticity criteria is the most straightforward and cheapest PCR based approach for testing a large number of coding region SNP-s, which greatly facilitates correct haplogroup determination...
2017: PloS One
https://www.readbyqxmd.com/read/28421949/vital-signs
#10
(no author information available yet)
A snapshot of the state of health and social care.
April 19, 2017: Nursing Standard
https://www.readbyqxmd.com/read/28420202/cereal-consumption-among-subjects-with-celiac-disease-a-snapshot-for-nutritional-considerations
#11
Francesco Valitutti, Donatella Iorfida, Caterina Anania, Chiara Maria Trovato, Monica Montuori, Salvatore Cucchiara, Carlo Catassi
BACKGROUND: To our knowledge no study has focused on the pattern of cereal-based products (CBP) consumption among people with celiac disease (CD). Our study aimed at evaluating the dietary intake of CBP among patients with CD and comparing it with a control population. METHODS: Eighty-two volunteers with CD and 77 non-CD volunteers enrolled throughout Italy were asked to register their consumption of CBP on specific diaries for three days. RESULTS: CD patients' median three-day intake of biscuits and crackers was higher compared to controls (65...
April 18, 2017: Nutrients
https://www.readbyqxmd.com/read/28420110/chemical-sensors-and-biosensors-in-italy-a-review-of-the-2015-literature
#12
REVIEW
Dario Compagnone, Girolamo Di Francia, Corrado Di Natale, Giovanni Neri, Renato Seeber, Antonella Tajani
The contributions of Italian researchers to sensor research in 2015 is reviewed. The analysis of the activities in one year allows one to obtain a snapshot of the Italian scenario capturing the main directions of the research activities. Furthermore, the distance of more than one year makes meaningful the bibliometric analysis of the reviewed papers. The review shows a research community distributed among different scientific disciplines, from chemistry, physics, engineering, and material science, with a strong interest in collaborative works...
April 14, 2017: Sensors
https://www.readbyqxmd.com/read/28420002/associations-between-stat-gene-polymorphisms-and-psoriasis-in-northeastern-china
#13
Jing Zhou, Yuzhen Li, Donglin Sun
BACKGROUND: Psoriasis is an autoimmune disease with genetic and environmental factors. Based on the roles of STATs (signaling transducers and activators of transcription) in autoimmune diseases, it is assumed STAT gene polymorphisms are associated with psoriasis. OBJECTIVES: To study the association between STAT gene polymorphisms and psoriasis in the northeastern Chinese population. METHODS: Eight single-nucleotide polymorphisms were genotyped: rs2293152, rs3816769, rs4796793, and rs744166 in STAT3, rs7574865 and rs3024866 in STAT4, and rs324011 and rs3024974 in STAT6, using SNaPshot methods...
April 19, 2017: Dermatology: International Journal for Clinical and Investigative Dermatology
https://www.readbyqxmd.com/read/28419903/dna-methylation-based-age-prediction-from-saliva-high-age-predictability-by-combination-of-7-cpg-markers
#14
Sae Rom Hong, Sang-Eun Jung, Eun Hee Lee, Kyoung-Jin Shin, Woo Ick Yang, Hwan Young Lee
DNA methylation is currently one of the most promising age-predictive biomarkers. Many studies have reported DNA methylation-based age predictive models, but most of these are based on DNA methylation patterns from blood. Only a few studies have examined age-predictive DNA patterns in saliva, which is one of the most frequently-encountered body fluids at crime scenes. In this study, we generated genome-wide DNA methylation profiles of saliva from 54 individuals and identified CpG markers that showed a high correlation between methylation and age...
April 9, 2017: Forensic Science International. Genetics
https://www.readbyqxmd.com/read/28419591/application-of-spin-echoes-in-the-regime-of-weak-dephasing-to-t1-mapping-of-the-lung
#15
Jakob Assländer, Steffen J Glaser, Jürgen Hennig
PURPOSE: This work presents an approach to mapping the entire lung's proton density and T1 within a single breath-hold and analyzes the apparent T1 when exciting with a spin echo generating pulse in comparison to a standard gradient echo acquisition. METHODS: An inversion-recovery SNAPSHOT-FLASH sequence with a stack-of-stars k-space readout with a golden angle increment was modified to use a spin echo generating radiofrequency-pulse for excitation. Data of five volunteers were acquired on a 3T scanner and image reconstruction was performed by an iterative algorithm adopted from MR-Fingerprinting...
April 17, 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
https://www.readbyqxmd.com/read/28418906/the-associations-of-genetic-polymorphisms-in-cyp1a2-and-cyp3a4-with-clinical-outcomes-of-breast-cancer-patients-in-northern-china
#16
Xianan Bai, Jingjing Xie, Shanshan Sun, Xianyu Zhang, Yongdong Jiang, Da Pang
BACKGROUND: Cytochrome P450 (CYP) 1A2 and CYP3A4 may play a role in the differentiation of clinical outcomes among breast cancer women. This study aimed to analyze the association of genetic polymorphisms in the CYP1A2 and CYP3A4 genes with clinicopathological features, protein expression and prognosis of breast cancer in the northern Chinese population. RESULTS: Firstly, SNP rs11636419, rs17861162 and rs2470890 in the CYP1A2 were significantly associated with age and menstruation status...
March 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28415941/endothelial-protein-c-receptor-polymorphisms-and-risk-of-sepsis-in-a-chinese-population
#17
Yanbing Liang, Xia Huang, Yujie Jiang, Yueqiu Qin, Dingwei Peng, Yuqing Huang, Jin Li, Suren R Sooranna, Liao Pinhu
Objective To examine the potential relationship of EPCR polymorphisms and the risk of sepsis in a Chinese population. Methods Snapshot SNP genotyping assays and DNA sequencing methods were used to detect polymorphisms of the EPCR gene, rs2069948C/T (2532C/T) and rs867186A/G (6936A/G), in 64 patients with sepsis and in 113 controls. Soluble EPCR (sEPCR) was measured by ELISA. Results There were significant differences in the allele and genotype frequencies of EPCR gene rs2069948C/T and allele frequencies of rs867186A/G between male and female patients and controls...
April 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/28415007/religion-spirituality-and-mental-health-of-u-s-military-veterans-results-from-the-national-health-and-resilience-in-veterans-study
#18
Vanshdeep Sharma, Deborah B Marin, Harold K Koenig, Adriana Feder, Brian M Iacoviello, Steven M Southwick, Robert H Pietrzak
BACKGROUND: In the last three decades, there has been increased interest in studying the association between religion/spirituality (R/S), and mental health and functional outcomes. METHODS: Using data from a contemporary, nationally representative sample of 3151 U.S. military veterans maintained by GfK Knowledge Networks, Inc., we evaluated the relation between R/S and a broad range of mental health, and psychosocial variables. Veterans were grouped into three groups based on scores on the Duke University Religion Index: High R/S (weighted 11...
April 11, 2017: Journal of Affective Disorders
https://www.readbyqxmd.com/read/28412135/moving-beyond-quality-control-in-diagnostic-radiology-and-the-role-of-the-clinically-qualified-medical-physicist
#19
REVIEW
H Delis, K Christaki, B Healy, G Loreti, G L Poli, P Toroi, A Meghzifene
Quality control (QC), according to ISO definitions, represents the most basic level of quality. It is considered to be the snapshot of the performance or the characteristics of a product or service, in order to verify that it complies with the requirements. Although it is usually believed that "the role of medical physicists in Diagnostic Radiology is QC", this, not only limits the contribution of medical physicists, but is also no longer adequate to meet the needs of Diagnostic Radiology in terms of Quality...
April 12, 2017: Physica Medica: PM
https://www.readbyqxmd.com/read/28410618/liquid-biopsy-a-step-forward-towards-precision-medicine-in-urologic-malignancies
#20
REVIEW
Ashley Di Meo, Jenni Bartlett, Yufeng Cheng, Maria D Pasic, George M Yousef
There is a growing trend towards exploring the use of a minimally invasive "liquid biopsy" to identify biomarkers in a number of cancers, including urologic malignancies. Multiple aspects can be assessed in circulating cell-free DNA, including cell-free DNA levels, integrity, methylation and mutations. Other prospective liquid biopsy markers include circulating tumor cells, circulating RNAs (miRNA, lncRNAs and mRNAs), cell-free proteins, peptides and exosomes have also emerged as non-invasive cancer biomarkers...
April 14, 2017: Molecular Cancer
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