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Resveratrol HepG2

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https://www.readbyqxmd.com/read/28983625/resveratrol-inhibits-proliferation-and-migration-through-sirt1-mediated-post%C3%A2-translational-modification-of-pi3k-akt-signaling-in-hepatocellular-carcinoma-cells
#1
Rongfei Chai, Huiling Fu, Zhaodi Zheng, Tingting Liu, Shuhua Ji, Guorong Li
Resveratrol (RES), a polyphenolic compound present in grapes and red wine, has potential anticancer properties. The present study aimed to examine the effects of resveratrol and its underlying mechanism on hepatocellular carcinoma (HCC) cell lines HepG2, Bel‑7402 and SMMC‑7721. It was demonstrated that resveratrol inhibited the viability and proliferation of HCC cells assessed by MTT and EdU assays. TUNEL assay revealed that resveratrol induced cell apoptosis by increasing HCC apoptosis rate from 3±0.78% to 16±1...
September 26, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28978315/resveratrol-enhances-anticancer-effects-of-paclitaxel-in-hepg2-human-liver-cancer-cells
#2
Qin Jiang, Manyi Yang, Zhan Qu, Jixiang Zhou, Qi Zhang
BACKGROUND: The aim of this in vitro study was to measure the enhanced anticancer effects of Res (resveratrol) on PA (paclitaxel) in HepG2 human liver cancer cells. METHODS: The MTT (thiazolyl blue tetrazolium bromide, 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), flow cytometry, qPCR (real-time quantitative polymerase chain reaction) and western blot assay were used for cells growth inhibitory effects, cells apoptosis (DNA content of sub-G1), mRNA and protein expressions, respectively...
October 4, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28795692/the-novel-resveratrol-derivative-3-5-diethoxy-3-4-dihydroxy-trans-stilbene-induces-mitochondrial-ros-mediated-er-stress-and-cell-death-in-human-hepatoma-cells-in-vitro
#3
Jae-Woo Park, Woo-Gyun Choi, Phil-Jun Lee, Su-Wol Chung, Byung-Sam Kim, Hun-Taeg Chung, Sungchan Cho, Jong-Heon Kim, Byoung-Heon Kang, Hyoungsu Kim, Hong-Pyo Kim, Sung-Hoon Back
Resveratrol (3,5,4'-trihydroxy-trans-stilbene) is a well-known polyphenol that is present in grapes, peanuts, pine seeds, and several other plants. Resveratrol exerts deleterious effects on various types of human cancer cells. Here, we analyzed the cell death-inducing mechanisms of resveratrol-006 (Res-006), a novel resveratrol derivative in human liver cancer cells in vitro. Res-006 was more effectively suppressed the viability of HepG2 human hepatoma cells than resveratrol (the IC50 values were 67.2 and 354...
August 10, 2017: Acta Pharmacologica Sinica
https://www.readbyqxmd.com/read/28768515/a-comparative-assessment-of-antiproliferative-properties-of-resveratrol-and-ethanol-leaf-extract-of-anogeissus-leiocarpus-dc-guill-and-perr-against-hepg2-hepatocarcinoma-cells
#4
COMPARATIVE STUDY
Jeremiah Olorunjuwon Olugbami, Robert Damoiseaux, Bryan France, Esther Modupe Onibiyo, Michael Adedapo Gbadegesin, Shivani Sharma, James Kazimierz Gimzewski, Oyeronke Adunni Odunola
BACKGROUND: Epidemiological and experimental evidences have shown cancer as a leading cause of death worldwide. Although the folklore use of plants as a reliable source of health-restoring principles is well-documented, the search for more of such plants that are active against diseases, such as cancer, continues. We report here a laboratory-based evidence of the relevance of an ethanol leaf extract of Anogeissus leiocarpus (A2L) in comparison with resveratrol, a natural polyphenol, in cancer therapy...
August 2, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28759727/the-beneficial-effects-of-resveratrol-on-steatosis-and-mitochondrial-oxidative-stress-in-hepg2-cells
#5
Magdalena Izdebska, Iwona Piątkowska-Chmiel, Agnieszka Korolczuk, Mariola Herbet, Monika Gawrońska-Grzywacz, Renata Gieroba, Marcin Sysa, Klaudia Czajkowska-Bania, Marcelina Cygal, Agnieszka Korga, Jarosław Dudka
Nonalcoholic fatty liver disease (NAFLD) is currently one of the most common chronic liver diseases, especially in developed countries. One group of substances with a potential use in the treatment of NAFLD are plant polyphenols, represented by resveratrol. The aim of this study was to evaluate the effect of resveratrol on steatosis and oxidative stress in HepG2 cells. The steatosis of cells was carried out using free fatty acids: oleic or palmitic acid and their mixtures. Steatosis was visualized using the intracellular lipid staining by Nile Red dye with a fluorescence microscope...
July 31, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28696482/cytotoxicity-and-genotoxicity-of-stilbene-derivatives-in-cho-k1-and-hepg2-cell-lines
#6
Cassia Suemi Mizuno, Winnifred Ampomaah, Fernanda Ribeiro Mendonça, Gabriela Carvalho Andrade, Ariel Maria Nazaré da Silva, Mirian Oliveira Goulart, Raquel Alves Dos Santos
The cytotoxicity and genotoxicity of the stilbenes (E)-methyl-4-(3-5-dimethoxystyryl)benzoate (ester), (E)-4-(3-5-dimethoxystyryl)aniline (amino), (Z)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (cis-TMS) and (E)-1,3-dimethoxy-5-(4-methoxystyryl)benzene (trans-TMS) were investigated in this work. Structural modifications of resveratrol, a naturally occurring stilbene, have been previously performed, including the replacement of hydroxyl by different functional groups. Such modifications resulted in significant improvement of target-specific effects on cell death and antiproliferative responses...
July 2017: Genetics and Molecular Biology
https://www.readbyqxmd.com/read/28686970/endocytic-pathways-of-optimized-resveratrol-cubosomes-capturing-into-human-hepatoma-cells
#7
Hend Mohamed Abdel-Bar, Rania Abd El Basset Sanad
Resveratrol (RSV) is a natural polyphenolic compound with high affinity to hepatocytes. It has numerous benefits as anticancer, antioxidant, immunomodulatory and cardioprotective actions. Nevertheless, RSV therapeutic applications are hindered by its low solubility, light sensitivity and extensive first-pass metabolism. Cubosomes are colloidally stable dispersed liquid crystalline nanoparticles. The incorporation of RSV into cubosomes could overcome some of its physicochemical limitations. A Design-Expert(®) software was applied to optimize cubosomes in terms of particle size and encapsulation efficiency (EE%)...
September 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28610486/resveratrol-loaded-glycyrrhizic-acid-conjugated-human-serum-albumin-nanoparticles-wrapping-resveratrol-nanoparticles-preparation-characterization-and-targeting-effect-on-liver-tumors
#8
Mingfang Wu, Bolin Lian, Yiping Deng, Ziqi Feng, Chen Zhong, Weiwei Wu, Yannian Huang, Lingling Wang, Chang Zu, Xiuhua Zhao
In this study, glycyrrhizic acid-conjugated human serum albumin nanoparticles wrapping resveratrol nanoparticles were prepared to establish a tumor targeting nano-sized drug delivery system. Glycyrrhizic acid was coupled to human serum albumin, and resveratrol was encapsulated in glycyrrhizic acid-conjugated human serum albumin by high-pressure homogenization emulsification. The average particle size of sample nanoparticles prepared under the optimal conditions was 108.1 ± 5.3 nm with a polydispersity index (PDI) of 0...
January 1, 2017: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/28557353/generation-of-carbamoyl-phosphate-synthetase-1-reporter-cell-lines-for-the-assessment-of-ammonia-metabolism
#9
Yi Wang, Le Chang, Jiahui Zhai, Qiao Wu, Donggen Wang, Yunfang Wang
Both primary hepatocytes and stem cells-derived hepatocyte-like cells (HLCs) are major sources for bioartificial liver (BAL). Maintenance of hepatocellular functions and induction of functional maturity of HLCs are critical for BAL's support effect. It remains difficult to assess and improve detoxification functions inherent to hepatocytes, including ammonia clearance. Here, we aim to assess ammonia metabolism and identify ammonia detoxification enhancer by developing an imaging strategy. In hepatoma cell line HepG2, and immortalized hepatic cell line LO2, carbamoyl phosphate synthetase 1 (CPS1) gene, the first enzyme of ammonia-eliminating urea cycle, was labelled with fluorescence protein via CRISPR/Cas9 system...
May 30, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28455791/resveratrol-inhibits-hepatocellular-carcinoma-progression-driven-by-hepatic-stellate-cells-by-targeting-gli-1
#10
Yu Yan, Cancan Zhou, Jie Li, Ke Chen, Guanghui Wang, Guangbing Wei, Mingwei Chen, Xuqi Li
Hepatocellular carcinoma (HCC) is characterized by hypervascularity. Hepatic stellate cells (HSCs) play very important roles in HCC malignant progression, as these cells facilitate HCC tumorigenesis and metastasis. We demonstrated that HSCs induce angiogenesis in HCC by upregulating the expression of Gli-1, which stimulates reactive oxygen species (ROS) production and potentiates increases in HCC cell invasiveness. Resveratrol abolished HSC-induced angiogenesis and suppressed ROS production and IL-6 and CXCR4 receptor expression in HepG2 cells by down-regulating Gli-1 expression...
April 28, 2017: Molecular and Cellular Biochemistry
https://www.readbyqxmd.com/read/28449683/inhibition-of-nampt-aggravates-high-fat-diet-induced-hepatic-steatosis-in-mice-through-regulating-sirt1-ampk%C3%AE-srebp1-signaling-pathway
#11
Ling-Fang Wang, Xiao-Nv Wang, Cong-Cong Huang, Long Hu, Yun-Fei Xiao, Xiao-Hui Guan, Yi-Song Qian, Ke-Yu Deng, Hong-Bo Xin
BACKGROUND: Nonalcoholic fatty liver disease is one of the most common liver diseases in the world and is a typical hepatic manifestation of metabolic syndrome which is characterized with lipid accumulation in liver. Nicotinamide phosphoribosyltransferase (NAMPT) has been recently identified as an enzyme involved in nicotinamide adenine dinucleotide (NAD(+)) biosynthesis and plays an important role in cellular metabolism in variety of organs in mammals. The aim of this study was to investigate the effects of NAMPT on high fat diet-induced hepatic steatosis...
April 27, 2017: Lipids in Health and Disease
https://www.readbyqxmd.com/read/28415630/resveratrol-alleviates-ffa-and-ccl4-induced-apoptosis-in-hepg2-cells-via-restoring-endoplasmic-reticulum-stress
#12
Fenghua Li, Yang Yang, Lili Yang, Kai Wang, Xing Zhang, Yunlong Zong, Yixin Ding, Changhong Wang, Li Zhang, Guang Ji
Cell apoptosis often induces inflammation and injury in the liver, with endoplasmic reticulum (ER) stress as the most possible reason. Resveratrol (RSV) has been shown to prevent hepatic steatosis and alleviate apoptosis, however, the exact mechanisms underlying the effects still need to be explored. Here we co-cultured HepG2 cells with free fatty acid (FFA) solution (oleic acid: palmitic acid = 2:1) and then exposed to a carbon tetrachloride (CCl4) solution to induce apoptosis. To evaluate the therapeutic effects, RSV (2...
July 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28317281/comparison-of-dietary-polyphenols-for-protection-against-molecular-mechanisms-underlying-nonalcoholic-fatty-liver-disease-in-a-cell-model-of-steatosis
#13
Hossein Rafiei, Kosar Omidian, Brian Bandy
SCOPE: Dietary polyphenols have shown promise in protecting the liver against nonalcoholic fatty liver disease. The relative effectiveness and mechanisms of different polyphenols however is mostly unknown. METHODS AND RESULTS: In a model of steatosis using HepG2 hepatocytes, we evaluated the protective effects of different classes of polyphenols and the contributing mechanisms. The treatment of the cells with oleic acid increased reactive oxygen species (ROS) generation and expression of tumor necrosis factor alpha (TNF-α), decreased expression of uncoupling protein 2, and decreased mitochondrial content and markers of biogenesis...
March 20, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28279696/hydroxystilbenes-and-methoxystilbenes-activate-human-aryl-hydrocarbon-receptor-and-induce-cyp1a-genes-in-human-hepatoma-cells-and-human-hepatocytes
#14
Barbora Pastorková, Aneta Vrzalová, Petr Bachleda, Zdeněk Dvořák
Natural polyphenol resveratrol (trihydroxystilbene) is a partial agonist of human aryl hydrocarbon receptor AhR, thereby, displaying a plethora of biological effects. Biological activities of metoxylated and hydroxylated stilbenes were studied in the past. The aim of the current study was to describe the effects of 13 different hydroxy- and methoxystilbenes, including their cis/trans isomers on the transcriptional activity of AhR and the expression of CYP1A genes in hepatic cancer cells HepG2 and in primary human hepatocytes...
May 2017: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/28216158/resveratrol-regulates-neuronal-glucose-uptake-and-insulin-sensitivity-via-p21-activated-kinase-2-pak2
#15
Pallavi Varshney, Chinmoy Sankar Dey
We have recently reported P21-activated kinase 2 (PAK2), a serine/threonine kinase as a negative regulator of neuronal glucose uptake and insulin sensitivity. Resveratrol (RSV), a natural polyphenol with anti-oxidative, anti-inflammatory and anti-diabetic properties, regulates PAK2 activity in HepG2 and ESC-B5 cell apoptosis. However, regulation of PAK2 by RSV in neuronal insulin signaling pathway, if any, is still unknown. In the present study, RSV treatment significantly increased PAK2 activity under insulin-sensitive and insulin-resistant condition, along with a marked decrease in glucose uptake in differentiated N2A cells...
April 1, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28197625/different-effect-of-resveratrol-to-induction-of-apoptosis-depending-on-the-type-of-human-cancer-cells
#16
Michinori Takashina, Sayaka Inoue, Kei Tomihara, Kengo Tomita, Kohshi Hattori, Qing-Li Zhao, Tokiko Suzuki, Makoto Noguchi, Wakana Ohashi, Yuichi Hattori
The effect of resveratrol on various human cancer cells was investigated with special focus on apoptotic cell death, in an attempt to further characterize its mechanism of action. There were great differences in the anti-viability effect of resveratrol between different types of human cancer cells. While the inhibition of cell viability by resveratrol was marked in U937 and MOLT-4 leukemia cells, resveratrol moderately inhibited cell viability in MCF-7 breast, HepG2 liver, and A549 lung cancer cells, and the effect was slight on cell viability in Caco-2, HCT116, and SW480 colon cancer cells...
March 2017: International Journal of Oncology
https://www.readbyqxmd.com/read/28024794/does-resveratrol-improve-insulin-signalling-in-hepg2-cells
#17
Marjan Norouzzadeh, Fatemehsadat Amiri, Ali Akbar Saboor-Yaraghi, Farnoosh Shemirani, Yas Kalikias, Loghman Sharifi, Monireh Sadat Seyyedsalehi, Maryam Mahmoudi
OBJECTIVES: Diabetes mellitus is a common metabolic disorder with high global prevalence. It is characterized by a decrease in insulin secretion or a decrease in insulin sensitivity or both. The aim of the present study was to investigate the effects of resveratrol treatment on the expression of the genes involved in insulin signalling cascade, such as Forkhead box protein O1 (FoxO1), 3-phosphoinositide-dependent protein kinase 1 (PDPK1) and mammalian target of rapamycin (mTOR). METHODS: HepG2 cells were cultured in serum-free medium with high concentrations of glucose and insulin and then were treated with resveratrol (5, 10 and 20 µM) for 24 and 48 hours...
April 2017: Canadian Journal of Diabetes
https://www.readbyqxmd.com/read/27748853/pterostilbene-inhibits-hepatocellular-carcinoma-through-p53-sod2-ros-mediated-mitochondrial-apoptosis
#18
Liying Guo, Kai Tan, Hao Wang, Xuan Zhang
Hepatocellular carcinoma (HCC) is one of the most common malignancies and the second cause of cancer-related deaths around the world. Pterostilbene (PTE), is a natural analog of resveratrol, possessing diverse pharmacological activities. In the present study, we aimed to examine the effect of PTE on tumor growth in mouse models of HCC and to elucidate the possible molecular mechanism in vivo and in vitro. We showed that PTE dose-dependently suppressed tumor growth in mice induced by diethylnitrosamine plus carbon tetrachloride, as evidenced by a decrease in the number of tumors and in the maximum size of the tumors...
December 2016: Oncology Reports
https://www.readbyqxmd.com/read/27735997/cholesterol-lowering-effects-and-mechanisms-in-view-of-bile-acid-pathway-of-resveratrol-and-resveratrol-glucuronides
#19
Dongyan Shao, Yilin Wang, Qingsheng Huang, Junling Shi, Hui Yang, Zhongli Pan, Mingliang Jin, Haobin Zhao, Xiaoguang Xu
Resveratrol (Res) was previously reported to be capable of lowering plasma TC and LDL-C. The mechanism behind Res is not clearly understood, although it is presumed to have an effect on bile acid metabolism in the liver: a significant way in eliminating cholesterol from the body. As one of the major metabolites of Res in the liver, resveratrol glucuronides (Gres) is suspected to also contribute to the overall cholestrol-lowering activity of Res, which needs to be studied. In this research, when HepG2 steatosis hepatic cells were treated with Res and Gres at different concentration levels, Res and Gres showed similar activity in lowering cellular TC content...
October 13, 2016: Journal of Food Science
https://www.readbyqxmd.com/read/27627919/resveratrol-ameliorates-alcoholic-fatty-liver-by-inducing-autophagy
#20
Liying Tang, Fengli Yang, Zhirui Fang, Chengmu Hu
Alcoholic fatty liver (AFL) is early stage of alcoholic liver disease, which can progress to steatohepatitis, fibrosis, and cirrhosis if alcohol consumption is continued. The pathogenesis of AFL is associated with excessive lipid accumulation in hepatocytes. Resveratrol (RES), a dietary polyphenol found in red wines and grapes, has been shown to have a hepatoprotective effect. Autophagy is a crucial physiological process in cellular catabolism that involves the regulation of lipid droplets. Autophagy maintains a balance between protein synthesis, degradation and self-recycling...
2016: American Journal of Chinese Medicine
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