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https://www.readbyqxmd.com/read/28933777/generation-and-use-of-a-humanized-bone-marrow-ossicle-niche-for-hematopoietic-xenotransplantation-into-mice
#1
Andreas Reinisch, David Cruz Hernandez, Katharina Schallmoser, Ravindra Majeti
Xenotransplantation is frequently used to study normal and malignant hematopoiesis of human cells. However, conventional mouse xenotransplantation models lack essential human-specific bone-marrow (BM)-microenvironment-derived survival, proliferation, and self-renewal signals for engraftment of normal and malignant blood cells. As a consequence, many human leukemias and other hematologic disorders do not robustly engraft in these conventional models. Here, we describe a complete workflow for the generation of humanized ossicles with an accessible BM microenvironment that faithfully recapitulates normal BM niche morphology and function...
October 2017: Nature Protocols
https://www.readbyqxmd.com/read/28932332/juvenile-psammomatoid-ossifying-fibroma-jpof-of-proximal-radius-a-rare-entity
#2
Jagadish Prabhu, Veena Nagaraj, Iftikhar Ahmed Mukhtar
BACKGROUND: Juvenile psammomatoid ossifying fibroma (JPOF) is a rare fibro-osseous lesion that usually occurs in the facial bones. The pathognomonic histopathologic feature is the presence of spherical ossicles, which are similar to psammoma bodies. It is considered to be a unique lesion because of its reported tendency to occur in children and adolescents and its tendency for locally aggressive growth. Because this lesion is aggressive in nature with high recurrence rate, early detection and complete surgical excision are essential...
2017: Open Orthopaedics Journal
https://www.readbyqxmd.com/read/28894812/caloric-testing-in-patients-with-heavy-or-light-cupula-of-the-lateral-semicircular-canal
#3
Hiroaki Ichijo
OBJECTIVE: To clarify whether the dysfunction of the lateral semicircular canal remain or not in patients with heavy or light cupula. STUDY DESIGN: Prospective case series. METHODS: The subjects were 19 patients with heavy cupula (3 males, 16 females; mean age, 62.8 years) and 14 patients with light cupula (5 males, 9 females; mean age, 63 years). Caloric testing (iced water) was carried out after complete disappearance of positional nystagmus...
December 2016: Laryngoscope Investigative Otolaryngology
https://www.readbyqxmd.com/read/28874066/the-role-of-the-accessory-malleolar-ossicles-and-malleolar-avulsion-fractures-in-lateral-ankle-ligament-reconstruction
#4
Matthew M Reiner, Jonathan J Sharpe
While it is well known that ankle sprains are one of the most common injuries in the United States, predictive factors regarding failure of conservative treatment are not well known. There are many biomechanical and epidemiological factors that play a role in recurrence and failure of conservative treatment, but most cases are able to be treated with immobilization and/or rest, ice, elevation, physical therapy, and bracing. We propose that one important risk factor is often overlooked simply due to the fact that a vast majority of these cases resolve without the need for surgery...
September 1, 2017: Foot & Ankle Specialist
https://www.readbyqxmd.com/read/28855950/sea-urchin-growth-dynamics-at-microstructural-length-scale-revealed-by-mn-labeling-and-cathodoluminescence-imaging
#5
Przemysław Gorzelak, Aurélie Dery, Philippe Dubois, Jarosław Stolarski
BACKGROUND: Fluorochrome staining is among the most widely used techniques to study growth dynamics of echinoderms. However, it fails to detect fine-scale increments because produced marks are commonly diffusely distributed within the skeleton. In this paper we investigated the potential of trace element (manganese) labeling and subsequent cathodoluminescence (CL) imaging in fine-scale growth studies of echinoderms. RESULTS: Three species of sea urchins (Paracentrotus lividus, Echinometra sp...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/28852952/atlas-based-segmentation-of-temporal-bone-anatomy
#6
Kimerly A Powell, Tong Liang, Brad Hittle, Don Stredney, Thomas Kerwin, Gregory J Wiet
PURPOSE: To develop a time-efficient automated segmentation approach that could identify critical structures in the temporal bone for visual enhancement and use in surgical simulation software. METHODS: An atlas-based segmentation approach was developed to segment the cochlea, ossicles, semicircular canals (SCCs), and facial nerve in normal temporal bone CT images. This approach was tested in images of 26 cadaver bones (13 left, 13 right). The results of the automated segmentation were compared to manual segmentation visually and using DICE metric, average Hausdorff distance, and volume similarity...
August 29, 2017: International Journal of Computer Assisted Radiology and Surgery
https://www.readbyqxmd.com/read/28834851/incidence-and-fusion-of-os-trigonum-in-a-healthy-pediatric-population
#7
Derrick M Knapik, Sahejmeet S Guraya, Jessica A Jones, Daniel R Cooperman, Raymond W Liu
BACKGROUND: Accessory ossicles off the talus (os trigonum) are a common radiographic finding seen within the foot with variable symptomatology in adult and pediatric patients. Although most studies have examined the incidence of these anatomic variants in a retrospective manner, few have longitudinally followed the behavior of os trigonum over time. The aim of this study was to investigate the incidence, mean age of appearance and fusion, and fusion rate of os trigonum in a large cohort of asymptomatic pediatric patients...
August 18, 2017: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/28828536/mdct-of-the-temporal-bone-and-audiological-findings-of-pediatric-acquired-cholesteatoma
#8
Asli Tanrivermis Sayit, Dilek Saglam, Hediye Pinar Gunbey, Mustafa Tastan, Cetin Celenk
Cholesteatoma is a benign epithelial lesion affecting the middle ear and/or mastoid process, causing otorrhea and hearing loss. Here, we retrospectively evaluated the temporal multidetector computed tomography and audiological findings of acquired cholesteatoma in children. Forty-three patients younger than 18 years old with middle ear acquired cholesteatoma were evaluated with regard to their clinical symptoms, temporal multidetector computed tomography findings, and audiometry results. The multidetector computed tomography findings were classified according to the site-ossicle-complication classification, and the relationships between the clinical, radiological, and audiological findings were evaluated...
August 21, 2017: European Archives of Oto-rhino-laryngology
https://www.readbyqxmd.com/read/28826948/digit-like-bony-anomaly-of-the-hindfoot-a-case-report
#9
Anna-Kathrin Leucht, Martin Wiewiorski
We report the case of an asymptomatic digit-like bony anomaly located on the plantar aspect of the calcaneus, which was incidentally found on radiographs of a 50-year-old male with a tibial shaft fracture. To the best of our knowledge, this is the first description of such an anomaly in the foot. The differential diagnosis includes accessory ossicles, polydactyly, heel spur, heterotopic ossification, osteochondroma, and pelvic digits.
August 18, 2017: Journal of Foot and Ankle Surgery: Official Publication of the American College of Foot and Ankle Surgeons
https://www.readbyqxmd.com/read/28822316/improved-middle-ear-soft-tissue-visualization-using-synchrotron-radiation-phase-contrast-imaging
#10
Mai Elfarnawany, Seyed Alireza Rohani, Soroush Ghomashchi, Daniel G Allen, Ning Zhu, Sumit K Agrawal, Hanif M Ladak
High resolution images are used as a basis for finite-element modeling of the middle-ear structures to study their biomechanical function. Commonly used imaging techniques such as micro-computed tomography (CT) and optical microscopy require extensive sample preparation, processing or staining using contrast agents to achieve sufficient soft-tissue contrast. We compare imaging of middle-ear structures in unstained, non-decalcified human temporal bones using conventional absorption-contrast micro-CT and using synchrotron radiation phase-contrast imaging (SR-PCI)...
August 5, 2017: Hearing Research
https://www.readbyqxmd.com/read/28790437/meniscal-ossicles-as-micro-ct-imaging-biomarker-in-a-rodent-model-of-antigen-induced-arthritis-a-synchrotron-based-x-ray-pilot-study
#11
Sandro Donato, Serena Pacilè, Federico Colombo, Chiara Garrovo, Simeone Dal Monego, Paolo Macor, Giuliana Tromba, Stefania Biffi
It is increasingly recognized that early detection of bone erosion plays an important role in the overall evaluation of rheumatoid arthritis and in the choice of the correct treatment approach. Since an appropriate use of imaging biomarkers in preclinical settings offers the prospect of smaller and optimized sample size, in the present study we define an anatomical imaging biomarker that could be objectively measured from micro-CT imaging data as an indicator of bone erosion in arthritis process. The well-characterized antigen-induced arthritis (AIA) model in rats was used...
August 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28769615/non-destructive-morphological-observations-of-the-fleshy-brittle-star-asteronyx-loveni-using-micro-computed-tomography-echinodermata-ophiuroidea-euryalida
#12
Masanori Okanishi, Toshihiko Fujita, Yu Maekawa, Takenori Sasaki
The first morphological observation of a euryalid brittle star, Asteronyx loveni, using non-destructive X-ray micro-computed tomography (µCT) was performed. The body of euryalids is covered by thick skin, and it is very difficult to observe the ossicles without dissolving the skin. Computed tomography with micrometer resolution (approximately 4.5-15.4 µm) was used to construct 3D images of skeletal ossicles and soft tissues in the ophiuroid's body. Shape and positional arrangement of taxonomically important ossicles were clearly observed without any damage to the body...
2017: ZooKeys
https://www.readbyqxmd.com/read/28737157/oral-neoplasms-in-pickhandle-barracuda-sphyraena-jello-from-india
#13
V Singaravel, A Gopalakrishnan, K Raja, R Vijayakumar, S Asrafuzzaman
We report the spontaneous occurrence of oral neoplasms in pickhandle barracuda Sphyraena jello Cuvier, 1829 from Parangipettai, on the southeast coast of India. A total of 11736 fish were examined, of which 43 were affected with oral tumours, with an overall prevalence of 0.37%. Gross and clinical symptoms included reddish to grayish-white distended tumourous growths on the gingiva, intra-oral bones and tongue. The tumours exhibited delayed eruption and intra- or extra-oral swelling, varied in consistency from extremely firm to fleshy and released mucinous material...
July 24, 2017: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/28717576/demodulation-of-quasi-quadrature-interferometric-signals-for-use-in-the-totally-implantable-hearing-aids
#14
Milos C Tomic, Zoran V Djinovic, Slobodan J Petricevic
We propose and experimentally prove an algorithm for demodulation of interferometric signals, modified for use in a totally implantable hearing aid device. A fiber optic configuration, which generates two quasi-quadrature signals by a passive 3x3 coupler, for a non-contact detection of the middle ear ossicle vibration is employed. We simulated the ossicle vibration and large movements and demonstrated the effectiveness of the algorithm to compensate changes of the signal DC values and the phase shift introduced by the coupler...
July 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28714118/body-wall-structure-in-the-starfish-asterias-rubens
#15
Liisa M Blowes, Michaela Egertová, Yankai Liu, Graham R Davis, Nick J Terrill, Himadri S Gupta, Maurice R Elphick
The body wall of starfish is composed of magnesium calcite ossicles connected by collagenous tissue and muscles and it exhibits remarkable variability in stiffness, which is attributed to the mechanical mutability of the collagenous component. Using the common European starfish Asterias rubens as an experimental animal, here we have employed a variety of techniques to gain new insights into the structure of the starfish body wall. The structure and organisation of muscular and collagenous components of the body wall were analysed using trichrome staining...
September 2017: Journal of Anatomy
https://www.readbyqxmd.com/read/28714106/prevalence-and-distribution-of-sesamoid-bones-and-accessory-ossicles-of-the-foot-as-determined-by-digital-tomosynthesis
#16
Bon San Koo, Yoonah Song, Seunghun Lee, Yoon-Kyoung Sung, Il-Hoon Sung, Jae-Bum Jun
We previously reported that digital tomosynthesis (DTS) is a reliable tool for evaluating bony structures of the hand. The current study aimed to identify the prevalence and distribution of sesamoid bones and accessory ossicles of the foot using DTS and to compare the results to those of conventional radiography (CR). Foot images (DTS, 213; CR, 44) of 213 patients taken at a tertiary hospital were retrospectively reviewed. Sesamoid bones were identified in the metatarsaophalangeal (MTP), distal interphalangeal (DIP) and interphalangeal (IP) joints of the great (I), second (II), third (III), fourth (IV), and fifth (V) toes...
July 16, 2017: Clinical Anatomy
https://www.readbyqxmd.com/read/28706834/screw-fixation-of-os-acetabuli-an-arthroscopic-technique
#17
Adrián Cuéllar, Xabier Albillos, Asier Cuéllar, Ricardo Cuéllar
An os acetabuli (OA) increases the contact area and surface area of the acetabulum and is important to maintain congruity of the hip joint. Thus preservation of this ossicle is important to prevent loss of contact area and ensure containment of the femoral head. We describe an all-arthroscopic approach to the fixation of OA with a compression screw. Initially, the fibrous tissue is debrided between the acetabular rim and the OA, a guidewire is placed through the OA up to the acetabular rim, and a screw is inserted over the wire...
June 2017: Arthroscopy Techniques
https://www.readbyqxmd.com/read/28684743/inner-ear-ossification-and-mineralization-kinetics-in-human-embryonic-development-microtomographic-and-histomorphological-study
#18
Céline Richard, Guillaume Courbon, Norbert Laroche, Jean Michel Prades, Laurence Vico, Luc Malaval
Little is known about middle and inner ear development during the second and third parts of human fetal life. Using ultra-high resolution Microcomputed Tomography coupled with bone histology, we performed the first quantitative middle and inner ear ossification/mineralization evaluation of fetuses between 17 and 39 weeks of gestational age. We show distinct ossification paces between ossicles, with a belated development of the stapes. A complete cochlear bony covering is observed within the time-frame of the onset of hearing, whereas distinct time courses of ossification for semicircular canal envelopes are observed in relation to the start of vestibular functions...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28680629/an-isolated-traumatic-fracture-of-the-malleus-handle
#19
Sertac Yetiser
Traumatic fracture of the malleus handle without tympanic membrane perforation is a rare incidence. Diagnosis of conductive hearing loss can only be confirmed with manipulation of the ossicles under sedation as computerized tomography is not informative. We present a 39-year-old lady with Q-tip injury when she get sudden phone call.
July 2017: Clinical Case Reports
https://www.readbyqxmd.com/read/28680503/adenomatous-tumors-of-the-middle-ear-a-literature-review
#20
REVIEW
Flavia Amarante Cardoso, Eduardo Machado Rossi Monteiro, Livia Bernardi Lopes, Marianna Novaes da Costa Avila, Bernardo Oliveira Scarioli
Introduction  Neuroendocrine adenomas of the middle ear (NAME) are uncommon causes of middle ear masses. Mostly limited to case reports and small series, the literature is poor in providing an overall assessment of these tumors. Objective  To review the current literature about all aspects of the disease, including its etiology, clinical manifestations, diagnosis, and treatment. Data Synthesis  The pathogenesis of adenomatous tumors of the middle ear is not clear yet. One potential explanation is that an undifferentiated pluripotent endodermal stem cell may still be present in the middle ear mucosal surface, and may be the origin of the tumors...
July 2017: International Archives of Otorhinolaryngology
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