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https://www.readbyqxmd.com/read/29767474/mesenchymal-stromal-cells-from-shwachman-diamond-syndrome-patients-fail-to-recreate-a-bone-marrow-niche-in-vivo-and-exhibit-impaired-angiogenesis
#1
Donatella Bardelli, Erica Dander, Cristina Bugarin, Claudia Cappuzzello, Alice Pievani, Grazia Fazio, Paolo Pierani, Paola Corti, Piero Farruggia, Carlo Dufour, Simone Cesaro, Marco Cipolli, Andrea Biondi, Giovanna D'Amico
Shwachman-Diamond syndrome (SDS) is a rare multi-organ recessive disease mainly characterised by pancreatic insufficiency, skeletal defects, short stature and bone marrow failure (BMF). As in many other BMF syndromes, SDS patients are predisposed to develop a number of haematopoietic malignancies, particularly myelodysplastic syndrome and acute myeloid leukaemia. However, the mechanism of cancer predisposition in SDS patients is only partially understood. In light of the emerging role of mesenchymal stromal cells (MSCs) in the regulation of bone marrow homeostasis, we assessed the ability of MSCs derived from SDS patients (SDS-MSCs) to recreate a functional bone marrow niche, taking advantage of a murine heterotopic MSC transplant model...
May 16, 2018: British Journal of Haematology
https://www.readbyqxmd.com/read/29764779/patients-over-60-years-of-age-have-poor-prognosis-in-facial-nerve-decompression-surgery-with-preserved-ossicular-chain
#2
Norio Kondo, Yukie Yamamura, Manabu Nonaka
OBJECTIVE: We report our retrospective study of the recovery rate of auditory ossicles preserved facial nerve decompression surgery via the transmastoid approach in cases of both an electroneurography score of < 10% and a Yanagihara score of ≤8 in Bell's palsy and Ramsay Hunt syndrome. MATERIALS AND METHODS: We retrospectively reviewed 47 patients who we were able to follow-up for more than 6 months following the onset of palsy. The recovery rate was defined by the Japan Society for Facial Nerve Research or the Yanagihara score...
April 2018: Journal of International Advanced Otology
https://www.readbyqxmd.com/read/29749423/common-accessory-ossicles-of-the-foot-imaging-features-pitfalls-and-associated-pathology
#3
Bimal Mayur Kumar Vora, Bak Siew Steven Wong
Accessory ossicles are normal variants that are frequently seen but often overlooked or misdiagnosed. Commonly seen at the foot and ankle, they may be mistaken for fracture fragments or loose bodies, or not be recognised as a cause of the patient's symptoms. This pictorial essay aimed to review commonly encountered accessory ossicles of the foot and ankle by highlighting anatomic landmarks and associated pathologic processes through the use of both basic and advanced imaging modalities, in an effort to increase awareness of these clinical entities...
April 2018: Singapore Medical Journal
https://www.readbyqxmd.com/read/29748449/risk-factors-of-tympanoplasties-in-long-term-observation
#4
Joanna Janiak-Kiszka, Wojciech Kaźmierczak, Kinga Lewandowska, Mateusz Grabowski, Henryk Kaźmierczak, Łukasz Kluczyński
OBJECTIVES: The aim of the study was to evaluate risk factors influencing the results of tympanoplasties on the base of material taken from the Department of Otolaryngology and Laryngological Oncology Collegium Medicum of Nicolaus Copernicus University in Bydgoszcz between 2004-2009. In this period 98 operations were done. The time from operation to hearing examination was 3 to 7 years, mean 5,43. Tympanoplastic operation were divided according to Tos classification. Measuring hearing results, tonal audiometry was done and mean air bone gap on four frequencies was assesed (500, 1000, 2000, 3000 Hz), according to AAO-HNS guidelines (1995)...
April 30, 2018: Otolaryngologia Polska
https://www.readbyqxmd.com/read/29738887/transcriptome-analysis-reveals-enrichment-of-genes-associated-with-auditory-system-in-swimbladder-of-channel-catfish
#5
Yujia Yang, Xiaozhu Wang, Yang Liu, Qiang Fu, Changxu Tian, Chenglong Wu, Huitong Shi, Zihao Yuan, Suxu Tan, Shikai Liu, Dongya Gao, Rex Dunham, Zhanjiang Liu
In aquatic organisms, hearing is an important sense for acoustic communications and detection of sound-emitting predators and prey. Channel catfish is a dominant aquaculture species in the United States. As channel catfish can hear sounds of relatively high frequency, it serves as a good model for study auditory mechanisms. In catfishes, Weberian ossicles connect the swimbladder to the inner ear to transfer the forced vibrations and improve hearing ability. In this study, we examined the transcriptional profiles of channel catfish swimbladder and other four tissues (gill, liver, skin, and intestine)...
April 30, 2018: Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics
https://www.readbyqxmd.com/read/29716749/new-technique-for-endoscopically-assisted-particulate-graft-reconstruction-of-the-mandible
#6
C Ferretti, J Reyneke, M Heliotis, U Ripamonti
The reconstruction of mandibular defects using particulate grafts is a proven technique that restores the osseous anatomy effectively. Secondary osseous reconstruction can be accomplished with endoscopic assistance and reduced-access incisions if an intermediate spacer is placed during resection. Two patients required reconstruction after resection of mandibular ameloblastomas. We used a modified protocol that involved the implantation of a graft of particulate corticocancellous bone after removal of the spacer, and prepared the recipient site under endoscopic guidance with small extraoral incisions...
April 28, 2018: British Journal of Oral & Maxillofacial Surgery
https://www.readbyqxmd.com/read/29705655/spatially-confined-induction-of-endochondral-ossification-by-functionalized-hydrogels-for-ectopic-engineering-of-osteochondral-tissues
#7
Chiara Stüdle, Queralt Vallmajó Martín, Alexander Haumer, Julien Guerrero, Matteo Centola, Arne Mehrkens, Dirk J Schaefer, Martin Ehrbar, Andrea Barbero, Ivan Martin
Despite the various reported approaches to generate osteochondral composites by combination of different cell types and materials, engineering of templates with the capacity to autonomously and orderly develop into cartilage-bone bi-layered structures remains an open challenge. Here, we hypothesized that the embedding of cells inducible to endochondral ossification (i.e. bone marrow derived mesenchymal stromal cells, BMSCs) and of cells capable of robust and stable chondrogenesis (i.e. nasal chondrocytes, NCs) adjacent to each other in bi-layered hydrogels would develop directly in vivo into osteochondral tissues...
April 21, 2018: Biomaterials
https://www.readbyqxmd.com/read/29690406/a-taxonomic-review-of-the-genus-astrodendrum-echinodermata-ophiuroidea-euryalida-gorgonocephalidae-with-description-of-a-new-species-from-japan
#8
Masanori Okanishi, Toshihiko Fujita
A revision of the genus Astrodendrum Döderlein (Ophiuroidea, Euryalida, Gorgonocephalidae) is based on 14 specimens, including the holotypes of Astrodendrum galapagense A. H. Clark and Astroconus capense Mortensen and a syntype of Gorgonocephalus sagaminus Döderlein. A new species Astrodendrum spinulosum is described from the Pacific coast of Japan. It is distinguished from its congeners in having conical external ossicles on aboral side of the disc; bulges on lateral edges of proximal arms; 1 terminal projection on each arm spine of proximal portion of the arm; 2 or 3 secondary teeth on each hook-shaped arm spine of distal portion of the arm...
March 8, 2018: Zootaxa
https://www.readbyqxmd.com/read/29690229/shallow-water-brittle-stars-echinodermata-ophiuroidea-from-ara%C3%A3-%C3%A3-bay-southeastern-brazil-with-spatial-distribution-considerations
#9
Renata A S Alitto, Maristela L Bueno, Pablo D B Guilherme, Maikon Di Domenico, Ana Beardsley Christensen, Michela Borges
The detailed study of arm ossicles, particularly the lateral arm plates, is providing valuable information in the elucidation of ophiuroid taxonomy. The present study describes in detail 16 species of brittle stars from Araçá Bay, Brazil. This information is used to construct the first interactive electronic key, providing a valuable resource for a broad range of researchers. Brittle stars families were divided into three groups based on their spatial distribution: i) infaunal species of intertidal and shallow subtidal belonging to Amphiuridae and Ophiactidae, ii) epizoic species belonging to Amphiuridae, Ophiactidae, and Ophiotrichidae and, iii) epifaunal species of the subtidal belonging to Ophiodermatidae and Hemieuryalidae...
April 5, 2018: Zootaxa
https://www.readbyqxmd.com/read/29690062/a-revision-of-holothuria-halodeima-kefersteinii-selenka-1867-and-the-revival-of-holothuria-inornata-semper-1868-from-sea-cucumbers-collected-in-mexico-and-central-america
#10
Magali Honey-escandÓn, Francisco A SolÍs-marÍn
In 1958 Deichmann synonymized Holothuria inornata Semper, 1868 with Holothuria kefersteinii (Selenka, 1867). This nomenclature has been adopted until now. However, some specimens recently collected from the Mexican Pacific matched the original description of H. inornata Semper (1868) whereas others matched the original description by Selenka (1867). A morphological, molecular and ecological study of the specimens was conducted to confirm the identification of these specimens. The morphological characters of our specimens were compared with those contained in the original descriptions and figures of the types of both species...
January 31, 2018: Zootaxa
https://www.readbyqxmd.com/read/29669196/development-of-the-human-incus-with-special-reference-to-the-detachment-from-the-chondrocranium-to-be-transferred-into-the-middle-ear
#11
Jose Francisco Rodríguez-Vázquez, Masahito Yamamoto, Shinichi Abe, Yukio Katori, Gen Murakami
The mammalian middle ear represents one of the most fundamental features defining this class of vertebrates. However, the origin and the developmental process of the incus in the human remains controversial. The present study seeks to demonstrate all the steps of development and integration of the incus within the middle ear. We examined histological sections of 55 human embryos and foetuses at 6 to 13 weeks of development. At 6 weeks of development (16 Carnegie Stage), the incus anlage was found to be lateral to this vein at the cranial end of the first pharyngeal arch...
April 18, 2018: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29668444/oblong-sesamoid-bone-appearance-into-the-nuchal-ligament-a-case-report
#12
Themistoklis Tzatzairis, Jannis V Papathanasiou, Athanasios Ververidis, Ioannis Kazakos, Georgios Drosos, Konstantinos Tilkeridis
Radio-opaque formations behind the spinous processes of the cervical spine are really rare. They were described in 1929 for the first time. Sesamoid ossicles are found close to a bone or a joint and are usually small. Some sesamoids form part of normal human anatomy, while others are normal anatomical variants. We report a case of a 73-year-old Caucasian male who visited the outpatient clinic complaining about chronic cervical pain. An oblong sesamoid accidentally discovered during routine anteroposterior (AP) and lateral x-ray, within the nuchal ligament (NL) at the C3-C6 spinous processes level...
March 1, 2018: Folia Medica
https://www.readbyqxmd.com/read/29643689/the-utility-of-tc-99m-hydroxymethylene-diphosphonate-single-photon-emission-computed-tomography-computed-tomography-in-symptomatic-os-trigonum
#13
Sharjeel Usmani, Noora Bin Essa, Fahad Marafi, Rashid Rasheed, Fareeda Al Kandari
Os trigonum is a congenital condition in which a bone ossicle fails to fuse to a secondary ossification center in the posterior aspect of the talar bone. Repetitive plantar flexion of the foot may elicit inflammation of the ossicles fibrous connective band, subsequently causing pain. It is common to misdiagnose os trigonum as an avulsion fracture. We report a case of a 23-year-old male with symptomatic os trigonum diagnosed on Tc-99m hydroxymethylene diphosphonate single-photon emission computed tomography-computed tomography (SPECT-CT)...
April 2018: Indian Journal of Nuclear Medicine: IJNM: the Official Journal of the Society of Nuclear Medicine, India
https://www.readbyqxmd.com/read/29502435/ossicular-chain-reconstruction-in-a-developing-country
#14
Es-Hak Bedri, Miriam Redleaf
OBJECTIVES: In Ethiopia, 2-stage operations with middle ear prostheses are economically unfavorable. We hypothesized that single-stage autologous ossiculoplasty results in acceptable tympanic membrane (TM) and hearing improvements in a setting of limited resources. METHODS: One hundred eighty-eight patients (197 ears) who underwent 1-stage autologous ossiculoplasty for ossicular dysfunction are presented. All but 14 of these ears also had perforations of the TM...
May 2018: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/29487659/progressive-retraction-of-a-fractured-os-peroneum-suggesting-repetitive-injury-to-the-peroneus-longus-tendon
#15
Jennifer L Favinger, Michael L Richardson, Felix S Chew
The os peroneum is an accessory ossicle within the peroneus longus tendon. Prior reports have discussed fracture of the os peroneum with associated tears of the peroneus longus tendon. When the ossicle fractures, there can be varying degrees of retraction of the tendon, which can be diagnosed by malposition of the ossicle or the ossicle fragments. We report a case of a man with recurrent eversion ankle injuries with progressive retraction of a fractured os peroneum, implying injuries to the superior and inferior peroneal retinacula and the peroneus longus tendon...
February 2018: Radiology Case Reports
https://www.readbyqxmd.com/read/29467266/oldest-known-multituberculate-stapes-suggests-an-asymmetric-bicrural-pattern-as-ancestral-for-multituberculata
#16
Julia A Schultz, Irina Ruf, Thomas Martin
Middle ear ossicles (malleus, incus, stapes) are known for few multituberculate taxa, and three different stapedial morphotypes have been suggested: (i) slender, columelliform and microperforate, (ii) robust and rod-like, and (iii) bicrural. Reinvestigation of Upper Jurassic (Kimmeridgian) mammalian petrosals from the Guimarota coal mine in central Portugal (Western Europe) revealed an asymmetric bicrural stapes (ABS) in the paulchoffatiid Pseudobolodon oreas The middle ear ossicles displaced inside the osseous vestibule were detected by a µCT analysis...
February 28, 2018: Proceedings. Biological Sciences
https://www.readbyqxmd.com/read/29456937/to-evaluate-and-compare-the-result-of-ossiculoplasty-using-different-types-of-graft-materials-and-prosthesis-in-cases-of-ossicular-discontinuity-in-chronic-suppurative-otitis-media-cases
#17
Suresh Kumar, Kanak Yadav, Tarun Ojha, Abhishek Sharma, Amit Singhal, Saurabh Gakhar
Chronic suppurative otitis media, with and without cholesteatoma, frequently results in disruption of the ossicular chain. The present study was designed to compare the outcome using autologous ossicle and titanium prosthesis in two groups of suitable patients presenting with chronic suppurative otitis media for middle ear reconstructive surgeries. A prospective study done from July 2012 to December 2013, at Mahatma Gandhi Medical College and Hospital, Sitapura, Jaipur (Rajasthan). The target group included patients with chronic suppurative otitis media admitted and planned for reconstructive middle ear surgery...
March 2018: Indian Journal of Otolaryngology and Head and Neck Surgery
https://www.readbyqxmd.com/read/29417895/dumbbell-shaped-ossicles-discovered-in-pedicellaria-of-flower-sea-urchins
#18
Masaki Tamori, Jun Koki, Tatsuo Motokawa
Sea urchins have a globiferous pedicellaria that stands from a test with a stalk on which lies a head made of three movable jaws with venom-injecting teeth. The globiferous pedicellariae of the flower sea urchin Toxopneustes pileolus, one of the most developed among sea urchins, are unique in that the jaws are provided with a jaw membrane that gives the pedicellaria an appearance of a flower when the jaws are open. We observed this membrane in an ionic liquid that does not require processes, such as fixation, dehydration, or coating with conductive materials, for observation with a scanning electron microscope...
February 2018: Zoological Science
https://www.readbyqxmd.com/read/29417191/-new-clinical-applications-for-laser-doppler-vibrometry-in-otology
#19
REVIEW
T Strenger, M Brandstetter, T Stark, F Böhnke
BACKGROUND: An instrument to measure vibration in the middle ear needs to be sensitive enough to detect displacement on a nanometer scale, yet not affect the vibration itself. Numerous techniques have been described in the literature, but laser Doppler vibrometry (LDV) has nowadays become established as the standard method in hearing research. OBJECTIVE: This article aims to present possible clinical applications of an LDV system in otology. MATERIALS AND METHODS: A commercially available single-point vibrometer was used...
April 2018: HNO
https://www.readbyqxmd.com/read/29410245/engineered-humanized-bone-organs-maintain-human-hematopoiesis-in-vivo
#20
Kristin Fritsch, Sébastien Pigeot, Xiaomin Feng, Paul E Bourgine, Timm Schroeder, Ivan Martin, Markus G Manz, Hitoshi Takizawa
Hematopoietic stem cells (HSCs) are maintained in a specialized bone marrow (BM) environment, the so-called HSC niche, that provides pivotal factors for their maintenance. Although the cellular and molecular components of the mouse BM HSC niche have been extensively studied using genetically modified animals, relatively little is known about the counterpart human BM niche components. We previously illustrated, with a developmental tissue engineering approach, that human adult BM-derived mesenchymal stromal cells (MSCs) can develop into human bone organs (so-called ossicles) through endochondral ossification in vivo and that these human ossicles are able to maintain functional mouse HSCs...
May 2018: Experimental Hematology
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