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Vein harvesting

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https://www.readbyqxmd.com/read/28433355/the-no-touch-saphenous-vein-graft-in-elderly-coronary-bypass-patients-with-multiple-comorbidities-is-a-promising-conduit-to-substitute-the-left-internal-thoracic-artery
#1
Ninos Samano, Håkan Geijer, Lennart Bodin, Mikael Arbeus, John D Mannion, Michael Dashwood, Domingos Souza
OBJECTIVES: We investigated the patency rates of no-touch saphenous vein grafts anastomosed to the left anterior descending artery compared with the left internal thoracic artery. Further, we compared the patency of no-touch vein grafts to the left anterior descending artery with the patency of no-touch vein grafts to other coronary arteries. METHODS: Of 2635 consecutive patients undergoing coronary artery bypass grafting between 2003 and 2008, 168 (6.3%) were given at least a saphenous vein graft to the left anterior descending artery to avoid harvesting complications in high-risk patients or in response to a left internal thoracic artery injury...
March 24, 2017: Journal of Thoracic and Cardiovascular Surgery
https://www.readbyqxmd.com/read/28427135/-effects-of-allogeneic-bone-marrow-mesenchymal-stem-cells-on-polarization-of-peritoneal-macrophages-in-rats-with-sepsis
#2
Y H Zheng, B Xiong, Y Y Deng, W Lai, S Y Zheng, H N Bian, Z A Liu, Z F Huang, C W Sun, H H Li, H M Luo, L H Ma, H X Chen
Objective: To explore the effects of allogeneic bone marrow mesenchymal stem cells (BMSCs) on polarization of peritoneal macrophages isolated from rats with sepsis induced by endotoxin/lipopolysaccharide (LPS). Methods: (1) BMSCs were isolated, cultured and purified from 5 SD rats with whole bone marrow adherent method. The third passage of cells were collected for morphologic observation, detection of expressions of stem cell surface markers CD29, CD44, CD45, and CD90 with flow cytometer, and identification of osteogenic and adipogenic differentiation...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
https://www.readbyqxmd.com/read/28420464/-effect-of-combination-therapy-with-propofol-and-hydrogen-rich-saline-on-organ-damage-and-cytokines-in-a-murine-model-of-sepsis
#3
Yunchuan Hong, Hongguang Chen, Yonghao Yu, Keliang Xie
OBJECTIVE: To investigate the effect of combining propofol with hydrogen on organ damage and inflammation of sepsis in cecal ligation and puncture (CLP) mice model. METHODS: One hundred and forty male C57BL/6 mice were randomly divided into groups (n = 28): sham group, CLP group, propofol group, H2 group, and propofol and H2 group. The sepsis was induced by CLP operation. Mice in sham group did the same operation with ligation and puncture. The mice of propofol group and propofol and H2 group were given 50 mg/kg propofol through tail vein at 1 hour and 6 hours after CLP and the mice of H2 group and propofol and H2 group were given 5 mL/kg H2-rich saline i...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28420460/-bone-marrow-mesenchymal-stem-cells-modulated-the-inflammatory-response-by-regulating-the-expression-of-il-4-and-rage-products-in-the-rats-with-mods
#4
Xia Zhou, Guanghui Xiu, Yichao Zhu, Xiaolei Chen, Wei Xiong, Xinghua Pan, Jie Sun, Bin Ling
OBJECTIVE: To investigate the underlying mechanism of bone marrow mesenchymal stem cells (BMSC) modulating the inflammatory response during the multiple organ dysfunction syndrome (MODS), especially the expression of inflammatory cytokines, which will provide new theoretical and experimental basis of MODS in clinic. METHODS: BMSC of Sprague-Dawley (SD) rat (female, 4 weeks) was extracted and cultivated, and the 4th passage were used in experimental study. According to the random number table, 60 female SD rats were divided into three groups (n = 20 per group): sham group, MODS group, BMSC group...
April 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28413469/repairing-effects-of-icam-1-expressing-mesenchymal-stem-cells-in-mice-with-autoimmune-thyroiditis
#5
Shifeng Ma, Xiuhui Chen, Lihui Wang, Ying Wei, Yongqing Ni, Yanan Chu, Yuanlin Liu, Heng Zhu, Rongxiu Zheng, Yi Zhang
The aim of the present study was to determine the repairing effects of intercellular adhesion molecule (ICAM)-1-expressing mesenchymal stem cells (MSCs) in mice with autoimmune thyroiditis. Following induction of an experimental autoimmune thyroiditis (EAT) model, mice were randomly divided into the following groups (n=10 each): i) Normal control; and experimental groups that were subject to EAT induction, including ii) EAT model; and iii) primary MSC; iv) C3H10T1/2/MSC; v) C3H10T1/2-MIGR1/MSC; and vi) C3H10T1/2-MIGR1-ICAM-1/MSC, which were all administered the relevant cells...
April 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28411705/mechanochemical-ablation-causes-endothelial-and-medial-damage-to-the-vein-wall-resulting-in-deeper-penetration-of-sclerosant-compared-with-sclerotherapy-alone-in-extrafascial-great-saphenous-vein-using-an-ex%C3%A2-vivo-model
#6
Mark S Whiteley, Scott J Dos Santos, Christopher T Lee, Jian-Mei Li
BACKGROUND: Nonthermal, tumescentless devices are the next generation of minimally invasive devices to treat varicose veins. We aimed to investigate the effects of mechanochemical ablation (MOCA) using ClariVein (Vascular Insights, Quincy, Mass) on ex vivo great saphenous vein with histology and immunofluorescent staining. METHODS: Extrafascial great saphenous veins were harvested during surgery for varicose veins and were treated ex vivo for 10 to 11 minutes with either liquid sclerotherapy or the use of ClariVein, with and without 3% sodium tetradecyl sulfate...
May 2017: Journal of Vascular Surgery. Venous and Lymphatic Disorders
https://www.readbyqxmd.com/read/28392314/persistence-and-proliferation-of-human-mesenchymal-stromal-cells-in-the-right-ventricular-myocardium-after-intracoronary-injection-in-a-large-animal-model-of-pulmonary-hypertension
#7
Roza Badr Eslam, Kevin Croce, Fernanda Marinho Mangione, Robert Musmann, Jane A Leopold, Richard N Mitchell, Aaron B Waxman
BACKGROUND AIMS: In this study, we demonstrate long-term persistence of human mesenchymal stromal cells (hMSCs) after intracoronary injection in a large animal model of pulmonary hypertension (PH). METHODS: Commercially available placenta-derived hMSCs were used. Experiments were conducted on 14 female Yorkshire swine. Four animals served as controls, and 10 underwent pulmonary vein (PV) banding. After 12 ± 2 weeks, PH and PV dysfunction were confirmed by right heart catheterization and cardiac magnetic resonance imaging...
April 6, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28389294/comparison-of-aspire-mechanical-thrombectomy-versus-angiojet-thrombectomy-system-in-a-porcine-iliac-vein-thrombosis-model
#8
Roy J Weinberg, Tamuru Okada, Aaron Chen, Walter Kim, Changyi Chen, Peter H Lin
PURPOSE: To compare the efficacy and safety profile of the AngioJet thrombectomy device and ASPIRE thrombectomy system in a porcine venous thrombosis model. MATERIALS AND METHODS: Twelve adult pigs underwent bilateral iliac venous thrombosis created by using a stent-graft thrombosis model, and subsequently underwent either AngioJet (n = 6) or ASPIRE mechanical thrombectomy (n = 6) one week later. Intravascular ultrasound (IVUS) was used to assess thrombectomy efficacy, and computed tomography was used to evaluate pulmonary embolism (PE)...
April 4, 2017: Annals of Vascular Surgery
https://www.readbyqxmd.com/read/28378316/domino-reconstruction-of-the-portal-vein-using-the-external-iliac-vein-and-an-eptfe-graft-in-pancreatic-surgery
#9
Masato Yoshioka, Hiroshi Uchinami, Go Watanabe, Masatake Iida, Yasuhiko Nakagawa, Hideaki Miyazawa, Makoto Yoshida, Yuzo Yamamoto
BACKGROUND: In the portal vein resection of long distance, an interposition by autologous vein is mandatory. External iliac vein (EIV) has been used, but harvesting the EIV is associated with severe venous congestion of the affected lower extremity. We have reconstructed the EIV using a ringed expanded polytetrafluoroethylene (ePTFE) graft. METHODS: Thirteen patients underwent this surgery. The right EIV was used for reconstructing the portal vein, and the retrieved portion of EIV was interposed by the ePTFE graft...
April 4, 2017: Journal of Gastrointestinal Surgery: Official Journal of the Society for Surgery of the Alimentary Tract
https://www.readbyqxmd.com/read/28362840/aspirin-triggered-resolvin-d1-attenuates-pdgf-induced-vascular-smooth-muscle-cell-migration-via-the-cyclic-adenosine-monophosphate-protein-kinase-a-camp-pka-pathway
#10
Giorgio Mottola, Anuran Chatterjee, Bian Wu, Mian Chen, Michael S Conte
BACKGROUND AND OBJECTIVES: Resolvin D1 (RvD1) is a specialized pro-resolving lipid mediator that has been previously shown to attenuate vascular smooth muscle cell (VSMC) migration, a key process in the development of intimal hyperplasia. We sought to investigate the role of the cAMP/PKA pathway in mediating the effects of the aspirin-triggered epimer 17R-RvD1 (AT-RvD1) on VSMC migration. METHODS: VSMCs were harvested from human saphenous veins. VSMCs were analyzed for intracellular cAMP levels and PKA activity after exposure to AT-RvD1...
2017: PloS One
https://www.readbyqxmd.com/read/28352597/vessel-remodeling-after-intima-to-intima-contact-anastomosis
#11
Hyeonjung Yeo, Hyodong Kim, Daegu Son, Changbae Hong, Sun Young Kwon
BACKGROUND: Intima-to-intima microanastomotic vascular remodeling was explored, utilizing a polylactide-caprolactone absorbable vein coupler model (PAVCM), which was designed to simulate a non-absorbable counterpart system with the sole exception of being absorbable. METHODS: Six New Zealand white rabbits were used. After transection of the jugular vein, 2 PAVCMs were placed, 1 at each transected end. The stumps were slipped through the PAVCMs, and the venous wall was everted 90° to achieve intima-to-intima contact...
March 2017: Archives of Plastic Surgery
https://www.readbyqxmd.com/read/28343765/human-cardiac-progenitor-cells-with-regenerative-potential-can-be-isolated-and-characterized-from-3d-electro-anatomic-guided-endomyocardial-biopsies
#12
Domenico D'Amario, Antonio Maria Leone, Maria Lucia Narducci, Costantino Smaldone, Dalgisio Lecis, Frediano Inzani, Marco Luciani, Andrea Siracusano, Federica La Neve, Melissa Manchi, Gemma Pelargonio, Francesco Perna, Piergiorgio Bruno, Massimo Massetti, Dario Pitocco, Donato Cappetta, Grazia Esposito, Konrad Urbanek, Antonella De Angelis, Francesco Rossi, Roberto Piacentini, Giulia Angelini, Domenica Donatella Li Puma, Claudio Grassi, Elisa De Paolis, Ettore Capoluongo, Valentina Silvestri, Biagio Merlino, Riccardo Marano, Filippo Crea
AIMS: In the present study, we aimed to develop a percutaneous approach and a reproducible methodology for the isolation and expansion of Cardiac Progenitor Cells (CPCs) from EndoMyocardial Biopsies (EMB) in vivo. Moreover, in an animal model of non-ischemic heart failure (HF), we would like to test whether CPCs obtained by this methodology may engraft the myocardium and differentiate. METHODS AND RESULTS: EMB were obtained using a preformed sheath and a disposable bioptome, advanced via right femoral vein in 12 healthy mini pigs, to the right ventricle...
March 1, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28331287/does-remifentanil-attenuate-renal-ischemia-reperfusion-injury-better-than-dexmedetomidine-in-rat-kidney
#13
E Erkılıç, E Kesimci, F Alaybeyoğlu, I Kılınç, R Tural, A Yazgan, T Gümüş, A Sepici Dinçel, E G Dumlu, O Kanbak
BACKGROUND: Ischemia-reperfusion (I/R) injury is a common cause of patient morbidity and mortality in the perioperative period. Patients undergoing long-lasting, abdominal, and urogenital surgeries with risk factors such as advanced age, peripheral artery disease, diabetes mellitus, renovascular disease, and congestive heart failure are candidates for acute kidney injury (AKI) due to impaired renal perfusion and decreased functional renal reserve. Pharmacological agents with multiple functions and anti-oxidative and anti-inflammation properties may be promising preventative strategies for AKI...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28329749/urethral-reconstruction-using-everted-saphenous-vein-graft-in-a-rabbit-model-one-year-outcomes
#14
Yujie Xu, Zhou Shen, Gang Liu, Bing Liu, Xiaoliang Hua, Yechen Xiang, Chuanjiang Dong, Bing Li
OBJECTIVE: To investigate the efficacy of using everted saphenous vein graft for urethral reconstruction. MATERIALS AND METHODS: Thirty-five adult male rabbits were divided into 7 groups randomly: experimental group A, B, C, D, E, stricture control group and normal control group (n = 5). In experimental groups and the stricture control group, a urethral mucosa defect (1.5 × 0.8 cm) was created in each rabbit. In experimental groups, a 2-cm long saphenous vein graft was harvested and incised longitudinally and urethral reconstruction was carried out using the everted saphenous vein patch...
March 23, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28328630/fluorescein-isothiocyanate-a-novel-application-for-lymphatic-surgery
#15
Lisa Spiguel, Christiana Shaw, Adam Katz, Lifei Guo, Hung-Chi Chen, Bernard T Lee, Dhruv Singhal
The Lymphatic Microsurgical Preventing Healing Approach (LYMPHA) procedure entails performing a lymphovenous bypass (LVB) at the time of axillary lymph node dissection to reduce lymphedema risk. The two most common fluorophores utilized in LVB are blue dye and indocyanine green. We developed a novel application of fluorescein isothiocyanate for intraoperative lymphatic mapping. Our goal is to demonstrate the safety and efficacy of fluorescein isothiocyanate for this application. We reviewed a prospectively collected database on breast cancer patients who underwent LYMPHA from March to September 2015...
March 21, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28322103/segmental-portal-superior-mesenteric-vein-resection-and-reconstruction-with-the-iliac-vein-after-pancreatoduodenectomy
#16
Xin Zhao, Li-Xin Li, Hua Fan, Jian-Tao Kou, Xian-Liang Li, Ren Lang, Qiang He
Objective The results of segmental venous resection (VR) combined with pancreatoduodenectomy (PD) are controversial but may be promising. Few studies have described reconstruction of the portal/superior mesenteric vein (PV/SMV) with the iliac vein harvested from donation after cardiac death (DCD). Methods From January 2014 to April 2016, PD combined with segmental excision of the PV/SMV (VR group) was performed in 21 patients with adenocarcinoma of the head of the pancreas (ADHP). The authors established a new technique of venous reconstruction using the iliac vein from DCD and analysed patients' long-term survival...
December 2016: Journal of International Medical Research
https://www.readbyqxmd.com/read/28318889/non-invasive-liver-ablation-using-histotripsy-preclinical-safety-study-in-an-in%C3%A2-vivo-porcine-model
#17
Eli Vlaisavljevich, Gabe Owens, Jonathan Lundt, Dejan Teofilovic, Kimberly Ives, Alexander Duryea, Jim Bertolina, Theodore H Welling, Zhen Xu
This study investigates the safety profile for use of histotripsy, a non-invasive ultrasonic ablation method currently being developed for the treatment of liver cancer, for liver ablation in an in vivo porcine model. Histotripsy treatments were applied to the liver and hepatic veins of 22 porcine subjects, with half of the subjects receiving systemic heparinization. Vital signs (heart rate, blood pressure, temperature, electrocardiogram and SpO2) were monitored throughout the procedure and for 1 h post-treatment...
March 16, 2017: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/28314066/comparison-of-propeller-perforator-flap-and-venous-supercharged-propeller-perforator-flap-in-reconstruction-of-lower-limb-soft-tissue-defect-a-prospective-study
#18
Benoit Chaput, Nicolas Bertheuil, Jean-Louis Grolleau, Farid Bekara, Raphael Carloni, Jerome Laloze, Christian Herlin
INTRODUCTION: In lower limbs, reliability of propeller perforator flaps (PPF) remains uncertain. The main complication is venous congestion, which can lead to distal necrosis. We aim to highlight if venous supercharging of PPF could substantially limit complications in lower limb coverage. METHODS: Between 2011 and 2016, we developed a standardized procedure of venous supercharging in the lower limb reconstruction with PPF using saphenous veins anastomosis. Then, we prospectively compared a consecutive series of 30 PPF to cover lower limbs defect, with a consecutive series of 30 venous-supercharged PPF (vsPPF)...
March 17, 2017: Microsurgery
https://www.readbyqxmd.com/read/28300869/e-selectin-inhibition-with-gmi-1271-decreases-venous-thrombosis-without-profoundly-affecting-tail-vein-bleeding-in-a-mouse-model
#19
Dorian L Culmer, Misha L Dunbar, Angela E Hawley, Suman Sood, Robert E Sigler, Peter K Henke, Thomas W Wakefield, John L Magnani, Daniel D Myers
Selectins, such as E-selectin (CD62E), function in venous thrombosis by binding and activating immune cells to initiate the coagulation cascade. GMI-1271 is a small molecule antagonist that inhibits E-selectin activity. Here we determine whether inhibition of E-selectin is sufficient to decrease acute venous thrombosis and associated inflammatory events in both prophylactic and treatment protocols without significantly affecting haemostasis. Male C57BL/6 mice underwent surgery for experimental thrombosis induction and were harvested at peak thrombus formation in our animal model, two days post induction...
March 16, 2017: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/28297834/-effect-of-inhibiting-perivascular-adhesion-on-intimal-hyperplasia-of-the-vein-grafts-in-rabbits
#20
M K Guo, J X Zhu, C M Han, T Bao, D Yang, P Yu, Y F Duan, F L Fan, H G Zhang, H J Tian
Objective: To investigate the effect of preventing perivascular adhesion with topical application of sodium hyaluronate on intimal hyperplasia of the vein grafts in rabbits. Methods: Twenty-four male New Zealand white rabbits, aged 5 months, were randomly divided into 2 groups: Group A and B (n=12 rabbits per group). Artery defect model was established by cutting about 1 cm artery from the middle part of the dissociated left common carotid artery. A section about 3 cm was cut from the right external jugular vein, and the harvested vein was inverted and end-to-end anastomosed to the artery defect...
March 7, 2017: Zhonghua Yi Xue za Zhi [Chinese medical journal]
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