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Vein harvesting

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https://www.readbyqxmd.com/read/29775572/gene-therapy-with-antisense-oligonucleotides-silencing-c-myc-reduces-neointima-formation-and-vessel-wall-thickness-in-a-mouse-model-of-vein-graft-disease
#1
Christina Maria Steger, Nikolaos Bonaros, Ralf Joachim Rieker, Johannes Bonatti, Thomas Schachner
Gene therapy for avoiding intimal hyperplasia of vein grafts after coronary artery bypass grafting is still discussed controversially. A promising application of gene therapy in vein grafts is the use of antisense oligonucleotides to block the expression of genes encoding cell cycle regulatory proteins in vascular smooth muscle cells. C-myc, either directly or by regulating the expression of other proteins, controls cell proliferation, apoptosis and cell survival, tissue remodeling, angiogenesis, cell metabolism, production of inflammatory and anti-inflammatory cytokines, and also participates in cell transformation...
May 15, 2018: Experimental and Molecular Pathology
https://www.readbyqxmd.com/read/29773466/in-vivo-morphologic-comparison-of-saphenous-vein-grafts-and-native-coronary-arteries-following-non-st-elevation-myocardial-infarction
#2
Tom Hsun-Wei Huang, Kalpa De Silva, Usaid K Allahwala, Edward J Danson, Pasi K Karjalainen, Olli A Kajander, Ravinay Bhindi
OBJECTIVE: This study aimed to assess the pathophysiological differences between saphenous vein grafts (SVG) and native coronary arteries (NCA) following presentation with non-ST elevated myocardial infarction (NSTEMI). BACKGROUND: There is accelerated pathogenesis of de novo coronary disease in harvested SVG following coronary artery bypass (CABG) surgery, which contributes to both early and late graft failure, and is also causal in adverse outcomes following vein graft PCI...
April 16, 2018: Cardiovascular Revascularization Medicine: Including Molecular Interventions
https://www.readbyqxmd.com/read/29764544/-effects-of-peroxisome-proliferator-activated-receptor-%C3%AE-toll-like-receptor-4-tumor-necrosis-factor-%C3%AE-targeted-pathway-on-hyperglycemia-induced-myocardium-inflammation-and-oxidative-stress
#3
Changle Liu, Ruimeng Liu, Xiaohan Wu, Peize Tan, Huaying Fu, Xinghua Wang, Tong Liu, Guangping Li
OBJECTIVE: To investigate the potential effects and mechanism on peroxisome proliferator-activated receptor γ-toll-like receptor 4-tumor necrosis factor-α (PPARγ-TLR4-TNF-α) targeted pathway on hyperglycemia induced myocardium inflammation and oxidative stress. METHODS: Thirty-two Japanese healthy adult rabbits were randomly divided into four groups with 8 rabbits in each group: normal control group (NC group), diabetes mellitus group (DM group), diabetes mellitus + pioglitazone 4 mg×kg-1 ×d-1 and 8 mg×kg-1 ×d-1 groups (DM+PGZ 4 mg and 8 mg groups)...
May 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/29755642/an-improved-method-for-increasing-the-efficiency-of-gene-transfection-and-transduction
#4
Baomin Shi, Mengzhou Xue, Yi Wang, Yufeng Wang, Davey Li, Xiaomin Zhao, Xinbo Li
Transfection and transduction using lentivirus has gained attention in biomedical research. To date, how to reach the maximum transfection and viral transduction efficiency is still challenging. Here we compared the transfection and viral transduction efficiency using commercially available transfection reagents including FuGENE 6, Lipofectamine 2000 and Lipofectamine 3000 in different cell lines and primary cultured cells. Enhanced green fluorescent protein (EGFP) was clearly seen in Eppendorf tubes from harvested cells using Lipofectamine 3000 without using a microscope and UV activation...
2018: International Journal of Physiology, Pathophysiology and Pharmacology
https://www.readbyqxmd.com/read/29753580/acceleration-of-diabetic-wound-healing-with-adipose-derived-stem-cells-endothelial-differentiated-stem-cells-and-topical-conditioned-medium-therapy-in-a-swine-model
#5
Robin F Irons, Kevin W Cahill, Deviney A Rattigan, Joseph H Marcotte, Marc W Fromer, Shaohua Chang, Ping Zhang, Eric M Behling, Kathryn C Behling, Francis J Caputo
OBJECTIVE: The purpose of our study was to investigate the effect of adipose-derived stem cells (ASCs), endothelial-differentiated ASCs (EC/ASCs), and various conditioned media (CM) on wound healing in a diabetic swine model. We hypothesized that ASC-based therapies would accelerate wound healing. METHODS: Diabetes was induced in four Yorkshire swine through intravenous injection of streptozotocin. ASCs were harvested from flank fat and cultured in either M199 or EGM-2 medium...
May 9, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/29748826/the-invention-of-microwave-surgical-scissors-for-seamless-coagulation-and-cutting
#6
Tohru Tani, Shigeyuki Naka, Soichiro Tani, Hisanori Shiomi, Koichiro Murakami, Atsushi Yamada, Dang Tran Khiem
PURPOSE: We developed a microwave energy-based scissors device (MWCX) that is capable of performing cutting and coagulation using 2.45 GHz microwave energy. This paper aims to present the concept of the device and assess the basic functions including the hemostasis, cutting, and sealing abilities. METHODS: Seven beagle dogs were used in our experiments. In six dogs, we measured the coagulation time (CT), lateral thermal injury (LTI), bursting pressure (BP). The dogs were then subjected to re-laparotomy 1 week later to allow us to investigate the results...
May 10, 2018: Surgery Today
https://www.readbyqxmd.com/read/29734909/the-observation-of-the-vein-distribution-of-a-partial-toe-transfer-flaps-with-a-short-vascular-pedicle
#7
Koichi Kobayashi, Katsuyasu Fukasawa, Naoko Masuyama
BACKGROUND: When performing partial toe-transfer flaps with a short vascular pedicle, as the flap becomes smaller, the likelihood of securing veins in the flap decreases. The purpose of this study was to clarify how frequently the partial toe-transfer flap with a short pedicle (free vascularized half-big toenail flap) contains veins and elucidate how frequently we can secure the veins with an artery via the first web space approach alone, using the Genial Viewer (a near-infrared light transmission imaging device)...
June 2018: Journal of Hand Surgery Asian-Pacific Volume
https://www.readbyqxmd.com/read/29721495/current-strategies-for-the-manufacture-of-small-size-tissue-engineering-vascular-grafts
#8
REVIEW
Michele Carrabba, Paolo Madeddu
Occlusive arterial disease, including coronary heart disease (CHD) and peripheral arterial disease (PAD), is the main cause of death, with an annual mortality incidence predicted to rise to 23.3 million worldwide by 2030. Current revascularization techniques consist of angioplasty, placement of a stent, or surgical bypass grafting. Autologous vessels, such as the saphenous vein and internal thoracic artery, represent the gold standard grafts for small-diameter vessels. However, they require invasive harvesting and are often unavailable...
2018: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/29718250/proximal-first-a-beneficial-strategy-for-no-touch-saphenous-vein-graft
#9
Azumi Hamasaki, Tetsuro Uchida, Masahiro Mizumoto, Jun Hayashi, Shuto Hirooka, Ai Ishizawa, Kentaro Akabane, Mitsuaki Sadahiro
In the mid-1990s, a novel saphenous vein harvesting technique, in which the vein is harvested with its surrounding tissue without manual distention, was introduced. This no-touch technique provides an excellent long-term patency; however, graft twisting and kinking should be given attention. To fully bring out the benefit of the no-touch method while reducing the risk of twisting and kinking, we have modified the anastomosis strategy. Our simple modified strategy involved a proximal anastomosis prior to the distal anastomosis...
April 27, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29709330/does-volumetric-measurement-of-cervical-lymph-nodes-serve-as-an-imaging-biomarker-for-locoregional-recurrence-of-oral-squamous-cell-carcinoma
#10
Ali-Farid Safi, Martin Kauke, Hendrik Jung, Marco Timmer, Jan Borggrefe, Thorsten Persigehl, Hans-Joachim Nickenig, Max Zinser, David Maintz, Matthias Kreppel, Joachim Zöller
In consideration of the 3-R-rule (Refine-Replace-Reduce) as a guideline for promoting ethical use of animals for surgical training, we present a novel training model for microvessel anastomosis. At hand of a rat cadaveric study, we evaluated the surgical anatomy of the common carotid artery (CCA), external jugular vein (EJV) and femoral vessels (FV) which were then used as templates for the present investigation. Anatomical dissection of 30 rat cadavers was performed. Two residents without prior microsurgical experience were included in the study and performed 5 CCA, 5 femoral artery, 5 EJV and 5 femoral vein anastomoses...
April 10, 2018: Journal of Cranio-maxillo-facial Surgery
https://www.readbyqxmd.com/read/29705656/humanization-of-bone-and-bone-marrow-in-an-orthotopic-site-reveals-new-potential-therapeutic-targets-in-osteosarcoma
#11
Ferdinand Wagner, Boris M Holzapfel, Jacqui A McGovern, Abbas Shafiee, Jeremy G Baldwin, Laure C Martine, Christoph A Lahr, Felix M Wunner, Thor Friis, Onur Bas, Melanie Boxberg, Peter M Prodinger, Ali Shokoohmand, Davide Moi, Roberta Mazzieri, Daniela Loessner, Dietmar W Hutmacher
BACKGROUND: Existing preclinical murine models often fail to predict effects of anti-cancer drugs. In order to minimize interspecies-differences between murine hosts and human bone tumors of in vivo xenograft platforms, we tissue-engineered a novel orthotopic humanized bone model. METHODS: Orthotopic humanized tissue engineered bone constructs (ohTEBC) were fabricated by 3D printing of medical-grade polycaprolactone scaffolds, which were seeded with human osteoblasts and embedded within polyethylene glycol-based hydrogels containing human umbilical vein endothelial cells (HUVECs)...
April 16, 2018: Biomaterials
https://www.readbyqxmd.com/read/29701407/creative-vascular-access-construction-in-same-patient
#12
Pedro Pinto Sousa, Paulo Almeida, Pedro Sá Pinto, Rui Almeida
INTRODUCTION: Creating and maintaining a functional vascular access(VA) is a critical factor in the survival of a dialysis patient. However, it will not function forever, implying a creative attitude from the vascular surgeon either to maintain its functionality or built a new one wherever possible, being it autologous or synthetic. METHODS: Describe the VA history of a 59years-old male with morbid obesity and end-stage chronic kidney disease. RESULTS: His VA construction started in2012 with failed attempts in both forearms until a functional brachiocephalic artery-venous fistula(AVF) in the right upper limb was achieved, but was deemed to ligation as severe venous hypertension secondary to central venous disease related to CVC use...
July 2017: Revista Portuguesa de Cirurgia Cardio-torácica e Vascular
https://www.readbyqxmd.com/read/29695021/pre-clinical-model-to-study-recurrent-venous-thrombosis-in-the-inferior-vena-cava
#13
Elizabeth A Andraska, Catherine E Luke, Megan A Elfline, Samuel P Henke, Siddharth S Madapoosi, Allan K Metz, Megan E Hoinville, Thomas W Wakefield, Peter K Henke, Jose A Diaz
BACKGROUND:  Patients undergoing deep vein thrombosis (VT) have over 30% recurrence, directly increasing their risk of post-thrombotic syndrome. Current murine models of inferior vena cava (IVC) VT model host one thrombosis event. OBJECTIVE:  We aimed to develop a murine model to study IVC recurrent VT in mice. MATERIALS AND METHODS:  An initial VT was induced using the electrolytic IVC model (EIM) with constant blood flow. This approach takes advantage of the restored vein lumen 21 days after a single VT event in the EIM demonstrated by ultrasound...
April 25, 2018: Thrombosis and Haemostasis
https://www.readbyqxmd.com/read/29688524/risk-factors-for-limb-surgical-site-infection-following-coronary-artery-bypass-graft-using-open-great-saphenous-vein-harvesting-a-retrospective-cohort-study
#14
Nasreen Hassoun-Kheir, Inon Hasid, Masha Bozhko, Ziyad Shaban, Roi Glam, Khetam Hussein, Mical Paul
OBJECTIVES: Our goal was to define risk factors for limb (leg) surgical site infections (SSIs) following coronary artery bypass grafting (CABG) with open saphenous vein grafting and to estimate their consequences for patients. METHODS: We performed a retrospective cohort study in a primary and tertiary hospital in Israel that included all adult patients undergoing CABG with open saphenous vein harvesting (November 2014-August 2016). Patients were followed perioperatively from admission until 90 days postoperatively, including post-discharge follow-up...
April 24, 2018: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/29687489/the-effect-of-storage-solutions-gene-therapy-and-antiproliferative-agents-on-endothelial-function-and-saphenous-vein-graft-patency
#15
Walid Ben Ali, Ismail Bouhout, Louis P Perrault
Vein graft failure remains a major concern after coronary artery bypass graft operations, and is initiated by loss of endothelial cell integrity. Preservation of saphenous vein grafts in the optimal solution after meticulous harvesting can limit the endothelial damage. Despite both experimental and clinical results in favor of buffered solutions, normal saline is still the most widely used solution. This review examines the literature to identify the most optimal storage solutions currently available for vein graft preservation...
May 2018: Journal of Cardiac Surgery
https://www.readbyqxmd.com/read/29682294/duragraft-vascular-conduit-preservation-solution-in-patients-undergoing-coronary-artery-bypass-grafting-rationale-and-design-of-a-within-patient-randomised-multicentre-trial
#16
Walid Ben Ali, Pierre Voisine, Peter Skov Olsen, Hugues Jeanmart, Nicolas Noiseux, Tracy Goeken, Vilas Satishchandran, Filippo Cademartiri, Garry Cutter, Dave Veerasingam, Craig Brown, Maximilian Y Emmert, Louis P Perrault
Introduction: Saphenous vein grafts (SVGs) remain the most often used conduits in coronary artery bypass grafting (CABG). However, they are prone to vein graft disease (VGD) during follow-up, which may compromise clinical outcomes. Injury to the SVG endothelium during harvesting and storage promotes neointimal hyperplasia that can advance to atherosclerosis characterised by SVG failure. This trial investigates the potential benefit of DuraGraft, a novel, one-time intraoperative graft treatment developed to efficiently protect the structural and functional integrity of the vascular endothelium, on the development and progression of VGD in CABG patients...
2018: Open Heart
https://www.readbyqxmd.com/read/29661800/chorionic-somatomammotropin-impacts-early-fetal-growth-and-placental-gene-expression
#17
Kimberly M Jeckel, Alexander C Boyarko, Gerrit Bouma, Quinton Winger, Russ V Anthony
Several developmental windows, including placentation, must be negotiated to establish and maintain pregnancy. Impaired placental function can lead to pre-eclampsia and/or intrauterine growth restriction (IUGR), resulting in increased infant mortality and morbidity. It has been hypothesized that chorionic somatomammotropin (CSH), plays a significant role in fetal development, potentially by modifying maternal and fetal metabolism. Recently, using lentiviral-mediated in vivo RNA interference in sheep, we demonstrated significant reductions in near-term (135 days of gestation; dGA) fetal and placental size, and altered fetal liver gene expression, resulting from CSH deficiency...
April 16, 2018: Journal of Endocrinology
https://www.readbyqxmd.com/read/29605267/effects-of-an-artificial-placenta-on-brain-development-and-injury-in-premature-lambs
#18
Joseph T Church, Nicole L Werner, Meghan A Coughlin, Julia Menzel-Smith, Mary Najjar, Benjamin D Carr, Hemant Parmar, Jeff Neil, Dimitrios Alexopoulos, Carlos Perez-Torres, Xia Ge, Scott C Beeman, Joel R Garbow, George B Mychaliska
PURPOSE: We evaluated whether brain development continues and brain injury is prevented during Artificial Placenta (AP) support utilizing extracorporeal life support (ECLS). METHODS: Lambs at EGA 118days (term=145; n=4) were placed on AP support (venovenous ECLS with jugular drainage and umbilical vein reinfusion) for 7days and sacrificed. Early (EGA 118; n=4) and late (EGA 127; n=4) mechanical ventilation (MV) lambs underwent conventional MV for up to 48h and were sacrificed, and early (n=5) and late (n=5) tissue control (TC) lambs were sacrificed at delivery...
March 8, 2018: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/29596344/treatment-of-arsenite-intoxication-induced-peripheral-vasculopathy-with-mesenchymal-stem-cells
#19
Yi-Hung Chiang, Chai-Chin Lin, Yen-Chung Chen, Oscar K Lee
Arsenite (As), a notorious toxic metal, is ubiquitously distributed in the earth and poses a serious threat to human health. Histopathological lesions of As intoxication are known as thromboangiitis obliterans, which are resistant to current treatment and often lead to lower limb amputation. In this study, we attempt to find that treatment with mesenchymal stem cells (MSCs) may be effective for As-induced vasculopathy. We first conducted an in vitro study with a co-culture system containing human MSCs and human umbilical vein endothelial cells (HUVECs) and treated individual and co-cultured cells with various concentrations of arsenite...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29595735/a-new-composite-eyeball-periorbital-transplantation-model-in-humans-an-anatomical-study-in-preparation-for-eyeball-transplantation
#20
Maria Siemionow, Mehmet Bozkurt, Fatih Zor, Yalcin Kulahci, Safak Uygur, Can Ozturk, Risal Djohan, Frank Papay
BACKGROUND: Vascularized composite allotransplantation offers a new hope for restoration of orbital content and perhaps vision. The aim of this study was to introduce a new composite eyeball-periorbital transplantation model in fresh cadavers in preparation for composite eyeball allotransplantation in humans. METHODS: The composite eyeball-periorbital transplantation flap borders included the inferior border, outlined by the infraorbital rim; the medial border, created by the nasal dorsum; the lateral border, created by the lateral orbital rim; and the superior border, created by the superior part of the eyebrow...
April 2018: Plastic and Reconstructive Surgery
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