keyword
https://read.qxmd.com/read/38542027/cholangiocyte-organoids-the-new-frontier-in-regenerative-medicine-for-the-study-and-treatment-of-cholangiopathies
#1
REVIEW
Serena Babboni, Pier Giuseppe Vacca, Ludovica Simonini, Daniele Pezzati, Caterina Martinelli, Francesco Frongillo, Giuseppe Bianco, Emanuele Marciano, Giuseppina Basta, Davide Ghinolfi, Serena Del Turco
Cholangiopathies include a group of chronic progressive disorders, affecting the cholangiocytes, the epithelial cells that line the biliary tree, leading to liver parenchymal fibrosis and eventually end-stage liver disease necessitating transplantation. Experimental modeling of these multifactorial cholestatic diseases faces challenges due to the lack of adequate experimental in vitro and in vivo models. A novel approach employs three-dimensional organoid systems that offer several advantages for modeling disease and testing drug response in vitro...
March 21, 2024: Journal of Clinical Medicine
https://read.qxmd.com/read/38539789/transcriptotype-driven-discovery-of-apigenin-as-a-therapy-against-cholestatic-liver-fibrosis-through-inhibition-of-panoptosis-and-following-type-i-interferon-responses
#2
JOURNAL ARTICLE
Shuni Duan, Xin Li, Junsong Han, Yang Yang, Ranyi Luo, Yajie Cai, Xiaojiaoyang Li, Qi Zheng, Jincheng Guo, Runping Liu
Cholangiopathies lack effective medicines and can progress into end-stage liver diseases. Mining natural product transcriptome databases for bioactive ingredients, which can reverse disease-associated transcriptomic phenotypes, holds promise as an effective approach for drug discovery. To identify disease-associated transcriptomic changes, we performed RNA-sequencing on bile duct ligation (BDL)-induced cholestatic liver fibrosis mice, as well as PBC and PSC patients, and found that PANoptosis and activation of type-I interferon (IFN) signaling were observed in BDL mice and patients with PBC and PSC...
February 20, 2024: Antioxidants (Basel, Switzerland)
https://read.qxmd.com/read/38535810/environmental-toxin-biliatresone-induced-biliary-atresia-like-abnormal-cilia-and-bile-duct-cell-development-of-human-liver-organoids
#3
JOURNAL ARTICLE
Yue Hai-Bing, Menon Sudheer Sivasankaran, Babu Rosana Ottakandathil, Wu Zhong-Luan, So Man-Ting, Chung Patrick Ho-Yu, Wong Kenneth Kak-Yuen, Tam Paul Kwong-Hang, Lui Vincent Chi-Hang
Biliary atresia (BA) is a poorly understood and devastating obstructive bile duct disease of newborns. Biliatresone, a plant toxin, causes BA-like syndrome in some animals, but its relevance in humans is unknown. To validate the hypothesis that biliatresone exposure is a plausible BA disease mechanism in humans, we treated normal human liver organoids with biliatresone and addressed its adverse effects on organoid development, functions and cellular organization. The control organoids (without biliatresone) were well expanded and much bigger than biliatresone-treated organoids...
March 11, 2024: Toxins
https://read.qxmd.com/read/38524667/laminin-511-e8-an-autoantigen-in-igg4-related-cholangitis-contributes-to-cholangiocyte-protection
#4
JOURNAL ARTICLE
David C Trampert, Remco Kersten, Dagmar Tolenaars, Aldo Jongejan, Stan F J van de Graaf, Ulrich Beuers
BACKGROUND & AIMS: IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease. Anti-laminin 511-E8 autoantibodies have been identified in its pancreatic manifestation. Laminin 511-E8 promotes endothelial barrier function, lymphocyte recruitment, and cholangiocyte differentiation. Here, we investigate anti-laminin 511-E8 autoantibody presence in IRC, and mechanisms via which laminin 511 may contribute to cholangiocyte protection. METHODS: Anti-laminin 511-E8 serum autoantibody positivity was assessed by ELISA...
April 2024: JHEP reports: innovation in hepatology
https://read.qxmd.com/read/38522506/neurotensin-contributes-to-cholestatic-liver-disease-potentially-modulating-matrix-metalloprotease-7
#5
JOURNAL ARTICLE
Hongxia Zhao, Xinbei Tian, Bo Wu, Ying Lu, Jun Du, Shicheng Peng, Yongtao Xiao
The diagnosis and treatment of biliary atresia pose challenges due to the absence of reliable biomarkers and limited understanding of its etiology. The plasma and liver of patients with biliary atresia exhibit elevated levels of neurotensin. To investigate the specific role of neurotensin in the progression of biliary atresia, the patient's liver pathological section was employed. Biliary organoids, cultured biliary cells, and a mouse model were employed to elucidate both the potential diagnostic significance of neurotensin and its underlying mechanistic pathway...
March 22, 2024: International Journal of Biochemistry & Cell Biology
https://read.qxmd.com/read/38519993/the-crosstalk-between-cholangiocytes-and-hepatic-stellate-cells-promotes-the-progression-of-epithelial-mesenchymal-transition-and-periductal-fibrosis-during-clonorchis-sinensis-infection
#6
JOURNAL ARTICLE
Junyeong Yi, Ji Hoon Jeong, Jihee Won, Seok Chung, Jhang Ho Pak
UNLABELLED: ABSTRACT: BACKGROUND: Clonorchis sinensis infection is one of the risk factors that provokes chronic inflammation, epithelial hyperplasia, periductal fibrosis and even cholangiocarcinoma (CCA). Disrupted or aberrant intercellular communication among liver-constituting cells leads to pathological states that cause various hepatic diseases. This study was designed to investigate the pathological changes caused by C. sinensis excretory-secretory products (ESPs) in non-cancerous human cell lines (cholangiocytes [H69 cell line] and human hepatic stellate cells [LX2 cell line]) and their intercellular crosstalk, as well the pathological changes in infected mouse liver tissues...
March 22, 2024: Parasites & Vectors
https://read.qxmd.com/read/38491381/affinity-fine-tuning-anti-caix-car-t-cells-mitigate-on-target-off-tumor-side-effects
#7
JOURNAL ARTICLE
Yufei Wang, Alicia Buck, Brandon Piel, Luann Zerefa, Nithyassree Murugan, Christian D Coherd, Andras G Miklosi, Haraman Johal, Ricardo Nunes Bastos, Kun Huang, Miriam Ficial, Yasmin Nabil Laimon, Sabina Signoretti, Zhou Zhong, Song-My Hoang, Gabriella M Kastrunes, Marion Grimaud, Atef Fayed, Hsien-Chi Yuan, Quang-De Nguyen, Tran Thai, Elena V Ivanova, Cloud P Paweletz, Ming-Ru Wu, Toni K Choueiri, Jon O Wee, Gordon J Freeman, David A Barbie, Wayne A Marasco
One of the major hurdles that has hindered the success of chimeric antigen receptor (CAR) T cell therapies against solid tumors is on-target off-tumor (OTOT) toxicity due to sharing of the same epitopes on normal tissues. To elevate the safety profile of CAR-T cells, an affinity/avidity fine-tuned CAR was designed enabling CAR-T cell activation only in the presence of a highly expressed tumor associated antigen (TAA) but not when recognizing the same antigen at a physiological level on healthy cells. Using direct stochastic optical reconstruction microscopy (dSTORM) which provides single-molecule resolution, and flow cytometry, we identified high carbonic anhydrase IX (CAIX) density on clear cell renal cell carcinoma (ccRCC) patient samples and low-density expression on healthy bile duct tissues...
March 16, 2024: Molecular Cancer
https://read.qxmd.com/read/38491300/opisthorchis-viverrini-excretory-secretory-products-suppress-glut8-of-cholangiocytes
#8
JOURNAL ARTICLE
Sandy Kha, Sujittra Chaiyadet, Prasert Saichua, Sirikachorn Tangkawatana, Banchob Sripa, Sutas Suttiprapa
Opisthorchis viverrini infection and the subsequent bile duct cancer it induces remains a significant public health problem in Southeast Asia. Opisthorchiasis has been reported to cause reduced plasma glucose levels among infected patients. The underlying mechanism for this phenomenon is unclear. In the present study, evidence is presented to support the hypothesis that O. viverrini exploits host cholangiocyte glucose transporters (GLUTs) in a similar manner to that of rodent intestinal nematodes, to feed on unabsorbed glucose in the bile for survival...
March 16, 2024: Parasitology Research
https://read.qxmd.com/read/38475870/cholangiocyte-organoids-to-study-drug-induced-injury
#9
JOURNAL ARTICLE
Zhenguo Wang, Chen Xing, Luc J W van der Laan, Monique M A Verstegen, Bart Spee, Rosalinde Masereeuw
BACKGROUND: Drug induced bile duct injury is a frequently observed clinical problem leading to a wide range of pathological features. During the past decades, several agents have been identified with various postulated mechanisms of bile duct damage, however, mostly still poorly understood. METHODS: Here, we investigated the mechanisms of chlorpromazine (CPZ) induced bile duct injury using advanced in vitro cholangiocyte cultures. Intrahepatic cholangiocyte organoids (ICOs) were driven into mature cholangiocyte like cells (CLCs), which were exposed to CPZ under cholestatic or non-cholestatic conditions through the addition of a bile acid cocktail...
March 13, 2024: Stem Cell Research & Therapy
https://read.qxmd.com/read/38475675/serum-itih5-as-a-novel-diagnostic-biomarker-in-cholangiocarcinoma
#10
JOURNAL ARTICLE
Meiru Chen, Jinghan Ma, Xiaoli Xie, Miao Su, Dongqiang Zhao
Cholangiocarcinoma often remains undetected until advanced stages due to the lack of reliable diagnostic markers. Our goal was to identify a unique secretory protein for cholangiocarcinoma diagnosis and differentiation from other malignancies, benign hepatobiliary diseases, and chronic liver conditions. We conducted bulk RNA-seq analysis to identify genes specifically upregulated in cholangiocarcinoma but not in most other cancers, benign hepatobiliary diseases, and chronic liver diseases focusing on exocrine protein-encoding genes...
March 12, 2024: Cancer Science
https://read.qxmd.com/read/38472011/danhongqing-formula-alleviates-cholestatic-liver-fibrosis-by-downregulating-long-non-coding-rna-h19-derived-from-cholangiocytes-and-inhibiting-hepatic-stellate-cell-activation
#11
JOURNAL ARTICLE
Meng Li, Yang Zhou, Hui Zhu, Lie-Ming Xu, Jian Ping
OBJECTIVE: This study explores the mechanism of action of Danhongqing formula (DHQ), a compound-based Chinese medicine formula, in the treatment of cholestatic liver fibrosis. METHODS: In vivo experiments were conducted using 8-week-old multidrug resistance protein 2 knockout (Mdr2-/- ) mice as an animal model of cholestatic liver fibrosis. DHQ was administered orally for 8 weeks, and its impact on cholestatic liver fibrosis was evaluated by assessing liver function, liver histopathology, and the expression of liver fibrosis-related proteins...
March 9, 2024: Journal of Integrative Medicine
https://read.qxmd.com/read/38469630/albumin-promoter-driven-flpo-expression-induces-efficient-genetic-recombination-in-mouse-liver
#12
JOURNAL ARTICLE
Xiaohui Zhu, Yan Yang, Dongfeng Feng, Oliver Wang, Jiaxiang Chen, Jiale Wang, Bin Wang, Yang Liu, Brandy H Edenfield, Ashley N Haddock, Ying Wang, Tushar Patel, Yan Bi, Baoan Ji
BACKGROUND AND AIMS: Tissue-specific gene manipulations are widely used in genetically engineered mouse models. A single recombinase system, such as the one using Alb-Cre, has been commonly used for liver-specific genetic manipulations. However, most diseases are complex, involving multiple genetic changes and various cell types. A dual recombinase system is required for conditionally modifying different genes sequentially in the same cell or inducing genetic changes in different cell types within the same organism...
March 12, 2024: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://read.qxmd.com/read/38466833/early-onset-of-pathological-polyploidization-and-cellular-senescence-in-hepatocytes-lacking-rad51-creates-a-pro-fibrotic-and-pro-tumorigenic-inflammatory-microenvironment
#13
JOURNAL ARTICLE
Wenqing Bu, Xue Sun, Xiaotong Xue, Shengmiao Geng, Tingting Yang, Jia Zhang, Yanan Li, Chao Feng, Qiao Liu, Xiyu Zhang, Peishan Li, Zhaojian Liu, Yufang Shi, Changshun Shao
BACKGROUND AIMS: RAD51 is a highly conserved DNA repair protein and is indispensable for embryonic viability. As a result, the role of RAD51 in liver development and function is unknown. Our aim was to characterize the function of RAD51 in postnatal liver development. APPROACH RESULTS: RAD51 is highly expressed during liver development and during regeneration following hepatectomy and hepatic injury, and is also elevated in chronic liver diseases. We generated hepatocyte-specific Rad51 deletion mouse model using Alb-Cre (Rad51-CKO) and AAV8-TBG-Cre to evaluate the function of RAD51 in liver development and regeneration...
March 11, 2024: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://read.qxmd.com/read/38465438/the-intriguing-connection-between-cholestasis-and-the-renin-angiotensin-system-dysregulation-induced-by-sars-cov-2-and-or-the-vaccinal-spike-protein
#14
EDITORIAL
Ziad Fajloun, Ziad Abi Khattar, Jean-Marc Sabatier
No abstract text is available yet for this article.
March 8, 2024: Infectious Disorders Drug Targets
https://read.qxmd.com/read/38460794/van-gogh-like-2-is-essential-for-the-architectural-patterning-of-the-mammalian-biliary-tree
#15
JOURNAL ARTICLE
Michaela Raab, Ersi Christodoulou, Roopesh Krishnankutty, Andreea Gradinaru, Alexander Daniel Walker, Paula Olaizola, Nicholas Thomas Younger, Anabel Martinez Lyons, Edward Joseph Jarman, Konstantinos Gournopanos, Alexander von Kriegsheim, Scott Hamilton Waddell, Luke Boulter
BACKGROUND & AIMS: In the developing liver, bipotent epithelial progenitor cells known as hepatoblasts undergo lineage segregation to form the two major epithelial cell types, hepatocytes that constitute the bulk of the liver parenchyma and biliary epithelial cells (cholangiocytes) which comprise the bile duct, a complex tubular network which is critical for normal liver function. Notch and TGFβ signalling promote the formation of a sheet of biliary epithelial cells, the ductal plate that organises into discontinuous tubular structures...
March 7, 2024: Journal of Hepatology
https://read.qxmd.com/read/38442326/4-3-pyridin-4-ylmethyl-amino-1-2-4-triazolo-4-3-b-1-2-4-triazin-6-yl-phenol-an-improved-anticancer-agent-in-hepatocellular-carcinoma-and-a-selective-mdr1-mrp-modulator
#16
JOURNAL ARTICLE
Zahra Zakeri Khatir, Antonella Di Sotto, Ester Percaccio, Tuba Tuylu Kucukkilinc, Ayse Ercan, Ann M Chippindale, Mehdi Valipour, Hamid Irannejad
Hepatocellular carcinoma is the most common type of primary liver cancer. However, multidrug resistance (MDR) is a major obstacle to the effective chemotherapy of cancer cells. This report documents the rational design, synthesis, and biological evaluation of a novel series of triazolotriazines substituted with CH2 NH-linked pyridine for use as dual c-Met/MDR inhibitors. Compound 12g with IC50 of 3.06 μM on HepG2 cells showed more potency than crizotinib (IC50  = 5.15 μM) in the MTT assay...
March 5, 2024: Archiv der Pharmazie
https://read.qxmd.com/read/38441998/antagonistic-effects-of-the-cytotoxic-molecules-granzyme-b-and-trail-in-the-immunopathogenesis-of-sclerosing-cholangitis
#17
JOURNAL ARTICLE
Mareike Kellerer, Sana Javed, Christian Casar, Nico Will, Laura K Berkhout, Dorothee Schwinge, Christian F Krebs, Christoph Schramm, Katrin Neumann, Gisa Tiegs
BACKGROUND AIMS: Primary sclerosing cholangitis (PSC) is a chronic cholestatic liver disease characterized by biliary inflammation and fibrosis. We showed an elevated interferon (IFN)γ response in PSC patients and in multidrug resistance protein 2-deficient (Mdr2-/-) mice developing sclerosing cholangitis. Interferon (IFN)γ induced expression of the cytotoxic molecules granzyme B (GzmB) and TNF-related apoptosis-inducing ligand (TRAIL) in hepatic lymphocytes and mediated liver fibrosis in sclerosing cholangitis...
March 5, 2024: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://read.qxmd.com/read/38439560/prenatal-prednisone-exposure-impacts-liver-development-and-function-in-fetal-mice-and-its-characteristics
#18
JOURNAL ARTICLE
Yongguo Dai, Yu Peng, Zhengjie Lu, Tongyun Mao, Kaiqi Chen, Xiaoqian Lu, Kexin Liu, Xinli Zhou, Wen Hu, Hui Wang
Prednisone, a widely used glucocorticoid drug in human and veterinary medicine, has been reported to cause developmental toxicity. However, systematic studies about the effect of prednisone on fetal liver development are still unclear. We investigated the potential effects of maternal exposure to clinically equivalent doses of prednisone during different gestational stages on cell proliferation and apoptosis, cell differentiation, glucose and lipid metabolism, and hematopoiesis in the liver of fetal mice, and explored the potential mechanisms...
March 4, 2024: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://read.qxmd.com/read/38436764/dynamic-yap-expression-in-the-non-parenchymal-liver-cell-compartment-controls-heterologous-cell-communication
#19
JOURNAL ARTICLE
Kaijing Liu, Lilija Wehling, Shan Wan, Sofia M E Weiler, Marcell Tóth, David Ibberson, Silke Marhenke, Adnan Ali, Macrina Lam, Te Guo, Federico Pinna, Fabiola Pedrini, Amruta Damle-Vartak, Anne Dropmann, Fabian Rose, Silvia Colucci, Wenxiang Cheng, Michaela Bissinger, Jennifer Schmitt, Patrizia Birner, Tanja Poth, Peter Angel, Steven Dooley, Martina U Muckenthaler, Thomas Longerich, Arndt Vogel, Mathias Heikenwälder, Peter Schirmacher, Kai Breuhahn
INTRODUCTION: The Hippo pathway and its transcriptional effectors yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are targets for cancer therapy. It is important to determine if the activation of one factor compensates for the inhibition of the other. Moreover, it is unknown if YAP/TAZ-directed perturbation affects cell-cell communication of non-malignant liver cells. MATERIALS AND METHODS: To investigate liver-specific phenotypes caused by YAP and TAZ inactivation, we generated mice with hepatocyte (HC) and biliary epithelial cell (BEC)-specific deletions for both factors (YAPKO, TAZKO and double knock-out (DKO))...
March 4, 2024: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/38428643/pten-deficiency-induces-an-extrahepatic-cholangitis-cholangiocarcinoma-continuum-via-aurora-kinase-a-in-mice
#20
JOURNAL ARTICLE
Yan Yang, Jiale Wang, Jianhua Wan, Qianqian Cheng, Zenong Cheng, Xueli Zhou, Oliver Wang, Kelvin Shi, Lingxiang Wang, Bin Wang, Xiaohui Zhu, Jiaxiang Chen, Dongfeng Feng, Yang Liu, Yasmin Jahan-Mihan, Ashley N Haddock, Brandy H Edenfield, Guang Peng, Jessica D Hohenstein, Chantal E McCabe, Daniel R O'Brien, Chen Wang, Sumera I Ilyas, Liuyan Jiang, Michael S Torbenson, Huamin Wang, Raouf E Nakhleh, Xuemei Shi, Ying Wang, Yan Bi, Gregory J Gores, Tushar Patel, Baoan Ji
BACKGROUND & AIMS: The PTEN-AKT pathway is frequently altered in extrahepatic cholangiocarcinoma (eCCA). We aim to evaluate the role of PTEN in the pathogenesis of eCCA and find novel therapies for this disease. METHODS: The Pten gene in the biliary epithelial cells were genetically deleted using the Cre-loxp system. The pathologies were evaluated both macroscopically and histologically. The characteristics were further analyzed by immunohistochemistry (IHC), RT-PCR, cell culture, and RNAseq...
February 28, 2024: Journal of Hepatology
keyword
keyword
65777
1
2
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.