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https://www.readbyqxmd.com/read/29453798/melatonin-protects-against-defects-induced-by-deoxynivalenol-during-mouse-oocyte-maturation
#1
Mei Lan, Jun Han, Meng-Hao Pan, Xiang Wan, Zhen-Nan Pan, Shao-Chen Sun
Deoxynivalenol (DON) is one of the most prevalent Fusarium mycotoxins in feedstuff and food. DON causes detrimental effects on human and animal reproductive systems by inducing oxidative stress and apoptosis. While melatonin is a multifunctional endogenous hormone which plays crucial roles in the development of animal germ cells and embryos as a robust deoxidizer. In present study, we explored the effects of melatonin on the DON-exposure mouse oocytes. Our in vitro and in vivo results showed that DON adversely affected mouse oocyte maturation and early embryo cleavage, while melatonin administration ameliorated the toxic effects of DON...
February 17, 2018: Journal of Pineal Research
https://www.readbyqxmd.com/read/29453161/gamma-irradiation-during-gametogenesis-in-young-adult-zebrafish-causes-persistent-genotoxicity-and-adverse-reproductive-effects
#2
Selma Hurem, Tânia Gomes, Dag A Brede, Ian Mayer, Viola H Lobert, Stephen Mutoloki, Kristine B Gutzkow, Hans-Christian Teien, Deborah Oughton, Peter Aleström, Jan L Lyche
The biological effects of gamma radiation may exert damage beyond that of the individual through its deleterious effects on reproductive function. Impaired reproductive performance can result in reduced population size over consecutive generations. In a continued effort to investigate reproductive and heritable effects of ionizing radiation, we recently demonstrated adverse effects and genomic instability in progeny of parents exposed to gamma radiation. In the present study, genotoxicity and effects on the reproduction following subchronic exposure during a gametogenesis cycle to 60 Co gamma radiation (27 days, 8...
February 13, 2018: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/29452915/effect-of-male-body-mass-index-on-assisted-reproduction-treatment-outcome-an-updated-systematic-review-and-meta-analysis
#3
REVIEW
Rabia Mushtaq, Jyotsna Pundir, Chiara Achilli, Osama Naji, Yacoub Khalaf, Tarek El-Toukhy
Men with a body mass index (BMI) of 30 or over are more likely to have reduced fertility and fecundity rates. This systematic review and meta-analysis evaluated the effect of male BMI on IVF and intracytoplasmic sperm injection (ICSI) outcome. An electronic search for published literature was conducted in MEDLINE and EMBASE between 1966 and November 2016. Outcome measures were clinical pregnancy rates (CPR) and live birth rates (LBR) per IVF or ICSI cycle. Eleven studies were identified, including 14,372 cycles; nine reported CPR and seven reported LBR...
February 2, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29448974/phyto-oestrogens-affect-fertilisation-and-embryo-development-in-vitro-in-sheep
#4
Anna Aryani Amir, Jennifer M Kelly, David O Kleemann, Zoey Durmic, Dominique Blache, Graeme B Martin
Phyto-oestrogens such as isoflavones are natural compounds that can profoundly affect reproductive function. In the present study, we tested whether including isoflavone compounds (genistein, biochanin A, formononetin) in the maturation medium would affect the outcomes for ovine oocytes in vitro. Each isoflavone compound was evaluated at five concentrations (0, 2.5, 5, 10, 25µgmL-1) and the entire protocol was repeated four times. Cumulus-oocyte complexes were randomly allocated to the treatments, then fertilised and cultured in vitro...
February 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29447380/sirtuins-in-gamete-biology-and-reproductive-physiology-emerging-roles-and-therapeutic-potential-in-female-and-male-infertility
#5
Carla Tatone, Giovanna Di Emidio, Arcangelo Barbonetti, Gaspare Carta, Alberto M Luciano, Stefano Falone, Fernanda Amicarelli
BACKGROUND: Sirtuins (SIRT1-7) are a family of NAD+-dependent deacetylases that catalyze post-translational modifications of proteins. Together, they respond to metabolic challenges, inflammatory signals or hypoxic/oxidative stress, and are associated with aging and longevity. The role of Sirtuins in the regulation of fertility emerged in 2003 when a defective reproductive phenotype was observed in SIRT1-null mice. Although studies on Sirtuins in reproductive biology have been increasing in the last years, a recent comprehensive update on this issue is still lacking...
February 13, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29447238/ex-situ-cultivation-protocol-for-cystoseira-amentacea-var-stricta-fucales-phaeophyceae-from-a-restoration-perspective
#6
Annalisa Falace, Sara Kaleb, Gina De La Fuente, Valentina Asnaghi, Mariachiara Chiantore
Due to multiple impacts, Cystoseira forests are experiencing a significant decline, which is affecting the ecosystem services they provide. Despite conservation efforts, there is an urgent need to develop best practices and large-scale restoration strategies. To implement restoration actions, we developed an ex situ protocol for the cultivation of Cystoseira. amentacea var. stricta, aimed at reducing the time needed for laboratory culture, thus avoiding prolonged maintenance and minimizing costs. Specifically, we tested the effects of temperature, light and substratum on settlement and growth of early life stages using a factorial experiment...
2018: PloS One
https://www.readbyqxmd.com/read/29447147/assisted-reproductive-technology-surveillance-united-states-2015
#7
Saswati Sunderam, Dmitry M Kissin, Sara B Crawford, Suzanne G Folger, Sheree L Boulet, Lee Warner, Wanda D Barfield
PROBLEM/CONDITION: Since the first U.S. infant conceived with assisted reproductive technology (ART) was born in 1981, both the use of ART and the number of fertility clinics providing ART services have increased steadily in the United States. ART includes fertility treatments in which eggs or embryos are handled in the laboratory (i.e., in vitro fertilization [IVF] and related procedures). Although the majority of infants conceived through ART are singletons, women who undergo ART procedures are more likely than women who conceive naturally to deliver multiple-birth infants...
February 16, 2018: MMWR. Surveillance Summaries: Morbidity and Mortality Weekly Report. Surveillance Summaries
https://www.readbyqxmd.com/read/29442466/relationship-between-resistin-and-il-23-levels-in-follicular-fluid-in-infertile-patients-with-endometriosis-undergoing-ivf-et
#8
Qun-Fang Zhang, Guo-Yong Chen, Yun Liu, Hui-Juan Huang, Yan-Feng Song
BACKGROUND: Endometriosis (EM) interferes with the reproductive process and affects the success rate of in vitro fertilization (IVF). Inflammatory cytokines are suggested to play a role in infertility in patients with EM. OBJECTIVES: In this study, we aimed to investigate the relationship between resistin and interleukin 23 (IL-23) levels in follicular fluid (FF) and serum together with the severity of endometriosis and in vitro fertilization/ embryo transfer (IVF-ET) outcome...
December 2017: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
https://www.readbyqxmd.com/read/29441061/profiling-of-human-molecular-pathways-affected-by-retrotransposons-at-the-level-of-regulation-by-transcription-factor-proteins
#9
Daniil Nikitin, Dmitry Penzar, Andrew Garazha, Maxim Sorokin, Victor Tkachev, Nicolas Borisov, Alexander Poltorak, Vladimir Prassolov, Anton A Buzdin
Endogenous retroviruses and retrotransposons also termed retroelements (REs) are mobile genetic elements that were active until recently in human genome evolution. REs regulate gene expression by actively reshaping chromatin structure or by directly providing transcription factor binding sites (TFBSs). We aimed to identify molecular processes most deeply impacted by the REs in human cells at the level of TFBS regulation. By using ENCODE data, we identified ~2 million TFBS overlapping with putatively regulation-competent human REs located in 5-kb gene promoter neighborhood (~17% of all TFBS in promoter neighborhoods; ~9% of all RE-linked TFBS)...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29440764/sperm-selection-by-thermotaxis-improves-icsi-outcome-in-mice
#10
Serafín Pérez-Cerezales, Ricardo Laguna-Barraza, Alejandro Chacón de Castro, María Jesús Sánchez-Calabuig, Esther Cano-Oliva, Francisco Javier de Castro-Pita, Luis Montoro-Buils, Eva Pericuesta, Raúl Fernández-González, Alfonso Gutiérrez-Adán
The ejaculate is a heterogeneous pool of spermatozoa containing only a small physiologically adequate subpopulation for fertilization. As there is no method to isolate this subpopulation, its specific characteristics are unknown. This is one of the main reasons why we lack effective tools to identify male infertility and for the low efficiency of assisted reproductive technologies. The aim of this study was to improve ICSI outcome by sperm selection through thermotaxis. Here we show that a specific subpopulation of mouse and human spermatozoa can be selected in vitro by thermotaxis and that this subpopulation is the one that enters the fallopian tube in mice...
February 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29436169/rethinking-developmental-toxicity-testing-evolution-or-revolution
#11
Anthony R Scialli, George Daston, Connie Chen, Prägati S Coder, Susan Y Euling, Jennifer Foreman, Alan M Hoberman, Julia Hui, Thomas Knudsen, Susan L Makris, LaRonda Morford, Aldert H Piersma, Dinesh Stanislaus, Kary E Thompson
BACKGROUND: Current developmental toxicity testing adheres largely to protocols suggested in 1966 involving the administration of test compound to pregnant laboratory animals. After more than 50 years of embryo-fetal development testing, are we ready to consider a different approach to human developmental toxicity testing? METHODS: A workshop was held under the auspices of the Developmental and Reproductive Toxicology Technical Committee of the ILSI Health and Environmental Sciences Institute to consider how we might design developmental toxicity testing if we started over with 21st century knowledge and techniques (revolution)...
February 12, 2018: Birth Defects Research
https://www.readbyqxmd.com/read/29432538/acquisition-of-oocyte-competence-to-develop-as-an-embryo-integrated-nuclear-and-cytoplasmic-events
#12
Marco Conti, Federica Franciosi
Infertility affects ~7% of couples of reproductive age with little change in incidence in the last two decades. ART, as well as other interventions, have made major strides in correcting this condition. However, and in spite of advancements in the field, the age of the female partner remains a main factor for a successful outcome. A better understanding of the final stages of gamete maturation yielding an egg that can sustain embryo development and a pregnancy to term remains a major area for improvement in the field...
February 8, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29430358/comparative-de-novo-transcriptome-analysis-of-male-and-female-sea-buckthorn
#13
Ankush Bansal, Mehul Salaria, Tashil Sharma, Tsering Stobdan, Anil Kant
Sea buckthorn is a dioecious medicinal plant found at high altitude. The plant has both male and female reproductive organs in separate individuals. In this article, whole transcriptome de novo assemblies of male and female flower bud samples were carried out using Illumina NextSeq 500 platform to determine the role of the genes involved in sex determination. Moreover, genes with differential expression in male and female transcriptomes were identified to understand the underlying sex determination mechanism...
February 2018: 3 Biotech
https://www.readbyqxmd.com/read/29430156/effect-of-endometrial-cavity-fluid-on-pregnancy-rate-of-fresh-versus-frozen-in-vitro-fertilization-cycle
#14
Nitika Gupta, Shilpa Bhandari, Pallavi Agrawal, Ishita Ganguly, Aparna Singh
Objective: This study aims to study the difference in etiology and outcome in terms of implantation rate and abortion rate in fresh (self-stimulated) versus frozen (oocyte donation cycle) in vitro fertilization (IVF) and in transient versus persistent fluid. Material and Methods: This retrospective study was conducted in the Department of Reproductive Medicine of tertiary care center from January 2012 to November 2015. Data were collected retrospectively from the departmental files...
October 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29430155/does-perifollicular-vascularity-on-the-day-of-oocyte-retrieval-affect-pregnancy-outcome-in-an-in-vitro-fertilization-cycle
#15
Nikita Naredi, Santosh Kumar Singh, Rajesh Sharma
Background: The vascularization status of ovarian follicles affects reproductive competence of oocytes and in turn embryo quality by regulating its oxygen supply. Transvaginal power Doppler ultrasound can noninvasively map this vascularity of the ovarian follicles. Thus, we aimed to study the association of perifollicular vascularity and pregnancy outcome in women while on treatment for an in vitro fertilization cycle. Material and Methods: A prospective study on 200 participants evaluated the vascularity of 1008 follicles on the day of oocyte retrieval to outline a map depicting perfusion of each follicle...
October 2017: Journal of Human Reproductive Sciences
https://www.readbyqxmd.com/read/29428314/gestational-carrier-in-assisted-reproductive-technology
#16
Gayathree Murugappan, Leslie V Farland, Stacey A Missmer, Katharine F Correia, Raymond M Anchan, Elizabeth S Ginsburg
OBJECTIVE: To compare clinical outcomes of in vitro fertilization (IVF) cycles with the use of gestational carriers (GCs) with non-GC IVF cycles. DESIGN: Retrospective cohort study of assisted reproductive technology (ART) cycles performed with (24,269) and without (1,313,452) the use of a GC. SETTING: ART centers. PATIENT(S): Infertile patients seeking IVF with or without use of a GC. INTERVENTIONS(S): Autologous and donor oocyte cycles, fresh and cryopreserved embryo transfer cycles...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29428313/clinical-outcomes-in-carriers-of-complex-chromosomal-rearrangements-a-retrospective-analysis-of-comprehensive-chromosome-screening-results-in-seven-cases
#17
Liang Hu, Yajing Wei, Keli Luo, Pingyuan Xie, Fei Gong, Bo Xiong, Yueqiu Tan, Guangxiu Lu, Ge Lin
OBJECTIVE: To evaluate the clinical outcomes in carriers of complex chromosomal rearrangements (CCRs). DESIGN: Case series. SETTING: An institute for reproductive and stem cell engineering. PATIENT(S): Seven couples with CCRs. INTERVENTION(S): Assisted reproduction with preimplantation genetic diagnosis (PGD). MAIN OUTCOME MEASURE(S): PGD results, embryo rating, pregnancy outcomes...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29423789/do-trained-reproductive-endocrinologists-perform-better-than-their-trainees-comparing-clinical-pregnancy-rates-and-live-birth-rates-after-transfer-of-single-fresh-blastocysts
#18
Sadikah Behbehani, Joseph Hasson, Stefano Polesello, W Y Son, Togas Tulandi, William Buckett
PURPOSE: To compare clinical pregnancy rates and live birth rates of single blastocyst transfers performed by attending physicians or fellows in reproductive endocrinology and infertility program. METHODS: Retrospective study in an academic reproductive center. We evaluated 932 fresh single blastocyst transfer cycles performed by fellows in training (389 embryo transfers) and by attending physicians (543 embryo transfers). RESULTS: There were no differences in the baseline characteristics and IVF cycle parameters between patients who had transfers performed by fellows or attending physicians...
February 9, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29421949/sperm-toxicity-testing-uk-best-practice-guideline-from-the-association-of-biomedical-andrologists
#19
S Long, B Woodward, M Tomlinson
In order to ensure the quality and integrity of diagnostic semen analysis results, materials used should be tested to ensure that they do not interfere with sperm function. As a toxicity test, complex sperm function testing may be considered controversial, since the fertilizing capacity of single sperm can never be assured. In preference, sperm motility offers a unique means of assessing the toxicity of reagents and materials before they are used in routine practice. Motility is the semen parameter most likely to be influenced by the external environment...
February 9, 2018: British Journal of Biomedical Science
https://www.readbyqxmd.com/read/29420669/insights-into-the-reproduction-of-some-antarctic-dendroceratid-poecilosclerid-and-haplosclerid-demosponges
#20
Vasiliki Koutsouveli, Sergi Taboada, Juan Moles, Javier Cristobo, Pilar Ríos, Andrea Bertran, Joan Solà, Conxita Avila, Ana Riesgo
Sponges are a dominant element of the Antarctic benthic communities, posing both high species richness and large population densities. Despite their importance in Antarctic ecosystems, very little is known about their reproductive patterns and strategies. In our study, we surveyed the tissue of six different species for reproductive elements, namely, Dendrilla antarctica Topsent, 1905 (order Dendroceratida), Phorbas areolatus (Thiele, 1905), Kirkpatrickia variolosa (Kirkpatrick, 1907), and Isodictya kerguelenensis (Ridley & Dendy, 1886) (order Poecilosclerida), and Hemigellius pilosus (Kirkpatrick, 1907) and Haliclona penicillata (Topsent, 1908) (Haplosclerida)...
2018: PloS One
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