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https://www.readbyqxmd.com/read/29679251/repressible-transgenic-sterilization-in-channel-catfish-ictalurus-punctatus-by-knockdown-of-primordial-germ-cell-genes-with-copper-sensitive-constructs
#1
Hanbo Li, Baofeng Su, Guyu Qin, Zhi Ye, Ahmed Elaswad, Ahmed Alsaqufi, Dayan A Perera, Zhenkui Qin, Ramji Odin, Khoi Vo, David Drescher, Dalton Robinson, Sheng Dong, Dan Zhang, Mei Shang, Nermeen Abass, Sanjay K Das, Max Bangs, Rex A Dunham
Repressible knockdown approaches were investigated to manipulate for transgenic sterilization in channel catfish, Ictalurus punctatus. Two primordial germ cell (PGC) marker genes, nanos and dead end, were targeted for knockdown and an off-target gene, vasa, was monitored. Two potentially copper-sensitive repressible promoters, yeast ctr3 (M) and ctr3-reduced (Mctr), were coupled with four knockdown strategies separately including: ds-sh RNA targeting the 5' end (N1) or 3' end (N2) of channel catfish nanos, full-length cDNA sequence of channel catfish nanos for overexpression (cDNA), and ds-sh RNA-targeting channel catfish dead end (DND)...
April 20, 2018: Marine Biotechnology
https://www.readbyqxmd.com/read/29677632/heat-shock-induced-cathepsin-b-activity-during-ivf-and-culture-compromises-the-developmental-competence-of-bovine-embryos
#2
Ken-Ichi Yamanaka, Hafiza Khatun, Junki Egashira, Ahmed Zaky Balboula, Hideki Tatemoto, Miki Sakatani, Naoki Takenouchi, Yasuhiko Wada, Masashi Takahashi
Heat stress can cause significant reproductive dysfunction in mammals and previous studies report that expression and activity of cathepsin B (CTSB), a lysosomal cysteine protease, is negatively correlated with the developmental competence of bovine oocytes and embryos. However, the relationship between heat shock (HS) and CTSB remains largely unknown. Here, we investigated the effects of HS during IVF and early embryonic stages of IVC on CTSB activity and developmental competence in bovine embryos. HS (40 °C for 6 h during IVF and 20 h during IVC) caused a significant increase in CTSB activity irrespective of the developmental stage or duration of HS...
April 6, 2018: Theriogenology
https://www.readbyqxmd.com/read/29676585/modified-hmg-stimulated-an-effective-option-in-endometrial-preparation-for-frozen-thawed-embryo-transfer-in-patients-with-normal-menstrual-cycles
#3
Pinxiu Huang, Lihong Wei, Xinlin Li, Zhong Lin
To evaluate the clinical efficacy of modified human menopausal gonadotropin (hMG) stimulated, hormone replacement therapy (HRT), natural cycling and letrozole ovulation induction during endometrial preparation for frozen-thawed embryo transfer (FET) in patients with normal menstrual cycles. This retrospective analysis included a total of 5070 cycles of patients with normal menstrual patterns who underwent FET between October 2009 and September 2015. The patients were divided into four groups according to the method of endometrial preparation for FET: 1838 cycles were natural, 1666 underwent HRT, 340 underwent letrozole ovulation induction and 1226 underwent modified hMG stimulated...
April 20, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29676492/the-effect-of-n-6-n-3-fatty-acid-ratios-on-broiler-breeder-performance-hatchability-fatty-acid-profile-and-reproduction
#4
A Khatibjoo, H Kermanshahi, A Golian, M Zaghari
This experiment was conducted to study the effect of dietary omega6 (n-6) to omega3 (n-3) fatty acid (FA) ratios on performance and reproduction of broiler breeders. In experiment 1, 400 females and 40 males (30 week age) of Ross 308 broiler breeder (20 females and two males in each pen) were randomly assigned to one of the four diets with n-6/n-3 FA ratios of 4, 6, 8 and 16 (control). As a measure of hatchability, fertility of eggs and general incubation traits, 1,200 eggs (60 eggs from each pen) were collected and incubated for 21 days and embryo liver and brain fatty acid profile in 14 and 21 days were determined...
April 20, 2018: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29676088/-explore-microcosmic-connection-between-autophagy-mechanism-and-follicular-development-based-on-kidney-governing-reproduction-theory
#5
REVIEW
Jun Bai, Ke-Ming Wu, Ran-Ran Gao
In the theory of traditional Chinese medicine(TCM) that "kidney storing essence and governing reproduction", reproductive essence is an important part of the kidney essence and acts as the original material of offspring embryos. Sperm, oocyte and zygote should be all included in the range of reproductive essence. Ovum is the essence of reproduction from inborn. The follicles maturation depends on the quality of oocyte and the vigor of kidney essence. Meanwhile, discharge of mature ovum relies on the stimulation and promotion by kidney Qi...
March 2018: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29676013/insemination-intrafollicular-fertilization-and-development-of-the-fertilization-plug-during-gestation-in-heterandria-formosa-poeciliidae
#6
Mari Carmen Uribe, Harry J Grier
The viviparous teleost Heterandria formosa is a remarkable species for its reproductive characters including: (a) the smallest oocyte in viviparous fish species; (b) a high level of matrotrophy with a complex placenta; and (c) the highest level of superfetation. Superfetation involves (d) the continuous development of oocytes and fertilization at the same time with embryos in gestation. The sequential fertilization of oocytes requires (e) storage of spermatozoa in the ovary. Among these characteristics, fertilization is of fundamental interest, specifically the intrafollicular fertilization of poeciliids, species that do not present micropyle, and the consequent formation of the fertilization plug, a structure developed at the periphery of the follicle where the entrance of spermatozoa occurs...
April 20, 2018: Journal of Morphology
https://www.readbyqxmd.com/read/29675488/trans-dermal-estrogen-oestrogel-for-endometrial-preparation-in-freeze-embryo-transfer-cycle-an-rct
#7
Ensieh Shahrokh Tehraninejad, Roya Kabodmehri, Batol Hosein Rashidi, Mina Jafarabadi, Fateme Keikha, Masomeh Masomi, Fedieh Hagholahi
Background: Estrogen and progesterone are two crucial factors for endometrial preparation in frozen embryo transfer (FET) cycles. Studies assessing different forms of estradiol in FET have published already but literature lacks enough surveys on transdermal estrogen application in reproductive medicine. Objective: To investigate the effects of trans dermal estrogen (Oestrogel) on pregnancy rates in patients that candidate for FET cycle. Materials and Methods: In this randomized clinical trial, 100 women undergoing FET cycles referred to Imam Khomeeini Hospital were enrolled in two groups, randomly...
January 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29675483/fresh-versus-frozen-embryo-transfer-after-gonadotropin-releasing-hormone-agonist-trigger-in-gonadotropin-releasing-hormone-antagonist-cycles-among-high-responder-women-a-randomized-multi-center-study
#8
Abbas Aflatoonian, Mahnaz Mansoori-Torshizi, Maryam Farid Mojtahedi, Behrouz Aflatoonian, Mohammaad Ali Khalili, Mohammad Hossein Amir-Arjmand, Mehrdad Soleimani, Nastaran Aflatoonian, Homa Oskouian, Nasim Tabibnejad, Peter Humaidan
Background: The use of embryo cryopreservation excludes the possible detrimental effects of ovarian stimulation on the endometrium, and higher reproductive outcomes following this policy have been reported. Moreover, gonadotropin-releasing hormone agonist trigger in gonadotropin-releasing hormone (GnRH) antagonist cycles as a substitute for standard human chorionic gonadotropin trigger, minimizes the risk of ovarian hyperstimulation syndrome (OHSS) in fresh as well as frozen embryo transfer cycles (FET)...
January 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29674245/effects-of-multiple-life-stage-exposure-to-the-fungicide-prochloraz-in-xenopus-laevis-manifestations-of-antiandrogenic-and-other-modes-of-toxicity
#9
Jonathan T Haselman, Patricia A Kosian, Joseph J Korte, Allen W Olmstead, Sigmund J Degitz
The Larval Amphibian Growth and Development Assay (LAGDA) is an internationally harmonized testing guideline for evaluating effects of chronic chemical exposure in amphibians. In order to evaluate the effects of chronic exposure to an antiandrogenic chemical in an amphibian model, prochloraz was tested using a variation of the LAGDA design. Exposure was initiated with <1d post-fertilization embryos at nominal concentrations of 0, 6.7, 20, 60 and 180 μg/L (0, 18, 53, 159, 478 nM) and continued in flow-through conditions until two months following the median time that controls completed metamorphosis...
April 3, 2018: Aquatic Toxicology
https://www.readbyqxmd.com/read/29674165/identification-expression-and-function-of-myosin-heavy-chain-family-genes-in-tribolium-castaneum
#10
Chengjun Li, Juanjuan Liu, Peng Lü, Shangshang Ma, Keming Zhu, Lu Gao, Bin Li, Keping Chen
Functions of myosin heavy chain (myosin) family genes are poorly understood in some insects. To address this, we determined the expression and function of myosin family genes in Tribolium castaneum. TcMyo15 is predominantly expressed in early embryos, late larvae and early adults, but TcMyo7B transcripts significantly increased in late larvae. TcMyo20 transcripts are abundant in pre-adults, whereas TcMhc1 is highly expressed in post-embryonic stages. TcMhc2 shows peak expression in late pupae. TcMyo9 transcripts reached their highest levels in late pupae...
April 16, 2018: Genomics
https://www.readbyqxmd.com/read/29673873/before-the-beginning-nutrition-and-lifestyle-in-the-preconception-period-and-its-importance-for-future-health
#11
REVIEW
Judith Stephenson, Nicola Heslehurst, Jennifer Hall, Danielle A J M Schoenaker, Jayne Hutchinson, Janet E Cade, Lucilla Poston, Geraldine Barrett, Sarah R Crozier, Mary Barker, Kalyanaraman Kumaran, Chittaranjan S Yajnik, Janis Baird, Gita D Mishra
A woman who is healthy at the time of conception is more likely to have a successful pregnancy and a healthy child. We reviewed published evidence and present new data from low-income, middle-income, and high-income countries on the timing and importance of preconception health for subsequent maternal and child health. We describe the extent to which pregnancy is planned, and whether planning is linked to preconception health behaviours. Observational studies show strong links between health before pregnancy and maternal and child health outcomes, with consequences that can extend across generations, but awareness of these links is not widespread...
April 16, 2018: Lancet
https://www.readbyqxmd.com/read/29670305/germline-genetic-modification-and-identity-the-mitochondrial-and-nuclear-genomes
#12
COMMENT
Rosamund Scott, Stephen Wilkinson
In a legal 'first', the UK removed a prohibition against modifying embryos in human reproduction, to enable mitochondrial replacement techniques (MRTs), a move the Government distanced from 'germline genetic modification', which it aligned with modifying the nuclear genome. This paper (1) analyzes the uses and meanings of this term in UK/US legal and policy debates; and (2) evaluates related ethical concerns about identity. It shows that, with respect to identity, MRTs and nuclear genome editing techniques such as CRISPR/Cas-9 (now a policy topic), are not as different as has been supposed...
December 2017: Oxford Journal of Legal Studies
https://www.readbyqxmd.com/read/29668864/bovine-embryo-elongation-is-altered-due-to-maternal-fatty-acid-supplementation
#13
K Giller, B Drews, J Berard, H Kienberger, M Schmicke, J Frank, B Spanier, H Daniel, G Geisslinger, S E Ulbrich
The pre-implantation period is prone to embryonic losses in bovine. Embryo-maternal communication is crucial to support embryo development. Thereby, factors of the uterine fluid (UF) are of specific importance. The maternal diet can affect the UF composition. Since omega 3 fatty acids (omega 3 FA) are considered to be beneficial for reproduction, we investigated if dietary omega 3 FA affected factors in the UF related to embryo elongation. Angus heifers (n = 37) were supplemented with either 450 g of rumen-protected fish oil (omega 3 FA) or sunflower oil (omega 6 FA) for a period of 8 weeks...
April 12, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29667547/new-markers-for-predicting-fertility-of-the-male-gametes-in-the-post-genomic-age
#14
Savina Dipresa, Luca De Toni, Carlo Foresta, Andrea Garolla
A number of test have been proposed to assess male fertility potential, ranging from routine testing by light microscopic method for evaluating semen samples, to screening test for DNA integrity aimed to look at sperm chromatin abnormalities. Spermatozoa are an extremely differentiated cell, they have critical functions for embryo development and heredity, in addiction to delivering a haploid paternal genome to the oocyte. Towards this goal certain requirements must always be met. The ability of spermatozoa to perform its reproductive function taking place in the spermatogenesis, a highly specialized process depending on multiple factors with effect on male fertility...
April 18, 2018: Protein and Peptide Letters
https://www.readbyqxmd.com/read/29665574/diagnostic-methods-to-detect-uterus-illnesses-in-mares
#15
Miguel Alejandro Silva Rua, Celia Raquel Quirino, Rachel Bittencourt Ribeiro, Eulogio Carlos Queiroz Carvalho, Maria de Lourdes Amaral Bernadino, Aylton Bartholazzi Junior, Luisa Fernanda Cipagalta, Marcus Antônio Pessanha Barreto
Uterine illnesses are the major problem in horse reproductive programs. The diagnosis of these pathologies is often neglected or does not provide enough information about the cause. The aim of this study was to evaluate different diagnoses to provide more accurate information about endometritis and the real endometrial profile at the beginning of a breeding season. Fifty-one mares with reproductive failure were evaluated. Two different uterine cytology diagnoses, culture of uterine flush material and biopsy assay, were performed...
April 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29664790/should-we-consider-telomere-length-and-telomerase-activity-in-male-factor-infertility
#16
Carlos Balmori, Elisa Varela
PURPOSE OF REVIEW: The purpose of this review is to analyze what is known to date about the relation between telomeres and male fertility, and if it is possible for telomeres, or elements related to them, to be used as new prognostic biomarkers in fertility treatment. RECENT FINDINGS: Cells in germ series, including spermatozoids, have longer telomeres (10-20 kb), and do not seem to undergo the shortening that takes place in somatic cells with age as they present telomerase activity...
April 16, 2018: Current Opinion in Obstetrics & Gynecology
https://www.readbyqxmd.com/read/29663591/research-progress-on-the-role-of-melatonin-and-its-receptors-in-animal-reproduction-a-comprehensive-review
#17
REVIEW
H S Talpur, I B Chandio, R D Brohi, T Worku, Z Rehman, D Bhattarai, F Ullah, L JiaJia, L Yang
Melatonin and its receptors play a crucial role in the regulation of the animal reproductive process, primarily in follicular development. However, the role that melatonin performs in regulating hormones related with reproduction remains unclear. Melatonin and its receptors are present both in female and male animals' organs, such as ovaries, heart, brain and liver. Melatonin regulates ovarian actions and is a key mediator of reproductive actions. Melatonin has numerous effects on animal reproduction, such as protection of gametes and embryos, response to clock genes, immune-neuroendocrine, reconciliation of seasonal variations in immune function, and silence or blockage of genes...
April 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29662078/comparison-of-the-life-history-parameters-and-competition-outcome-with-moina-macrocopa-between-two-morphs-of-brachionus-forficula
#18
Ya-Li Ge, Tong Luo, Cui-Cui Ge, Rong Zhan, Jin-Hang Yu, Yi-Long Xi, Gen Zhang
In rotifers, the costs of morphological defenses, especially the development of long spines, have been investigated for several decades. However, the obtained results were inconsistent and the underlying reasons were complicated. Investigations on more species might be helpful to find out the reasons. In the present study, Brachionus forficula was selected as the model organism. The differences in developmental durations, life-table demography, starvation resistant time and the competitive ability with Moina macrocopa were compared between B...
April 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29660629/fatty-acid-profile-of-blood-plasma-and-oviduct-and-uterine-fluid-during-early-and-late-luteal-phase-in-the-horse
#19
B Drews, V Milojevic, K Giller, S E Ulbrich
During early pregnancy, the secretome of both oviduct and uterus serves as exchange medium for signaling factors between embryo and mother and provides the embryo with nutrients. The preimplantation embryo can utilize the fatty acids (FA) therein via direct incorporation into cell membrane lipid bilayers and for energy production via β-oxidation. The FA concentration and composition of the oviduct (OF) and uterine fluid (UF) might be regulated by ovarian hormones to meet the changing needs of the growing embryo...
April 7, 2018: Theriogenology
https://www.readbyqxmd.com/read/29659034/embryo-fetal-toxicity-assessment-of-vonoprazan-in-rats-and-rabbits
#20
Tianyi Li, Hongqun Qiao, Peng Yue, Ming Cai, Xuejun He
Vonoprazan is a new potassium-competitive acid blocker to treat acid-related diseases. However, its safety during pregnancy is unclear. The aim of the study was to investigate the potential reproductive toxicity on the embryo-fetal development of vonoprazan. Vonoprazan acetate was administered by intravenous injection to pregnant rats (0, 2, 6 and 20 mg kg-1 day-1 ) and rabbits (0, 1.2, 3.6 and 12 mg kg-1 day-1 ) during the organogenetic period (gestation day 6-15 [rats] and 6-18 [rabbits]). Maternal reproductive endpoints were evaluated, together with effects on fetal growth and morphological development...
April 16, 2018: Journal of Applied Toxicology: JAT
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