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https://www.readbyqxmd.com/read/29346023/fertility-preservation-in-pediatric-oncology-patients-new-perspectives
#1
Chiara Gertosio, Mariasole Magistrali, Paola Musso, Cristina Meazza, Mauro Bozzola
Over the past 30 years, advances in antineoplastic treatment led to a significant increase in the survival of patients with childhood cancer. In Europe and the United States, 82% of children, adolescents, and young adults survive 5 years from the cancer diagnosis and the majority achieves long-term survival into adulthood. The impact of cancer therapy on fertility is related to the age of the patient and to the duration, dose/intensity, and type of treatment. Exposure to chemotherapy or to radiation to gonads or pituitary brings long-term complications of cancer-directed therapies that include effects on reproductive capacity...
January 18, 2018: Journal of Adolescent and Young Adult Oncology
https://www.readbyqxmd.com/read/29345445/the-impact-of-high-progesterone-levels-on-the-day-of-hcg-administration-in-assisted-human-reproduction-treatments
#2
Michelli S Tanada, Ivan H Yoshida, Monise Santos, Caroline Z Berton, Elen Souto, Waldemar P de Carvalho, Emerson B Cordts, Caio P Barbosa
OBJECTIVE: Progesterone is a steroid hormone that acts on the endometrium. It is known for producing physical and mood-related side effects. Few studies have looked into how progesterone levels affect embryo development and quality. This study aimed to find a cutoff level for serum progesterone on the day of HCG administration from which embryo quality is impaired. METHODS: The study included 145 cycles, from which 885 oocytes and 613 embryos were obtained. All patients had their serum progesterone levels measured on the day of HCG administration...
January 18, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29345310/a-potential-role-of-knockout-serum-replacement-as-a-porcine-follicular-fluid-substitute-for-in-vitro-maturation-lipid-metabolism-approach
#3
Jun-Xue Jin, Sanghoon Lee, Erif Maha Nugraha Setyawan, Anukul Taweechaipaisankul, Geon A Kim, Ho Jae Han, Curie Ahn, Byeong Chun Lee
The use of supplements, such as porcine follicular fluid (pFF), fetal bovine serum and human serum albumin are widely used during in vitro maturation (IVM) in different species but these supplements contain undefined components that cause technical difficulties in standardization and influence the efficiency of IVM. Knockout serum replacement (KSR) is a synthetic protein source, without any undefined growth factors or differentiation-promoting factors. Therefore, it is feasible to use KSR as a defined component for avoiding effects of unknown molecules in an IVM system...
January 18, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/29344847/the-effect-of-surgical-management-of-endometrioma-on-the-ivf-icsi-outcomes-when-compared-with-no-treatment-a-systematic-review-and-meta-analysis
#4
M Nickkho-Amiry, R Savant, K Majumder, E Edi-O'sagie, M Akhtar
OBJECTIVE: To assess the impact of surgical management of endometrioma on the outcome of assisted reproduction treatment (ART). DESIGN: A systematic review and meta-analysis. SETTING: Department of reproductive medicine at teaching university hospital, UK. PATIENTS: Subfertile women with endometrioma undergoing ART. INTERVENTIONS: Surgical removal of endometrioma or expectant management. MAIN OUTCOME MEASURES: Clinical pregnancy rate, pregnancy rate, live birth rate, number of oocytes retrieved and number of embryos available and ovarian response to gonadotrophins...
January 17, 2018: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29340017/altered-gnas-imprinting-due-to-folic-acid-deficiency-contributes-to-poor-embryo-development-and-may-lead-to-neural-tube-defects
#5
Li Wang, Shaoyan Chang, Zhen Wang, Shan Wang, Junsheng Huo, Gangqiang Ding, Rui Li, Chi Liu, Shaofang Shangguan, Xiaolin Lu, Ting Zhang, Zhiyong Qiu, Jianxin Wu
Disturbed epigenetic modifications have been linked to the pathogenesis of Neural Tube Defects (NTDs) in those with folate deficiency during pregnancy. However, evidence is lacking to delineate the critical region in epigenome regulated by parental folic acid and mechanisms by which folate deficiency affects normal embryogenesis. Our data from clinical samples revealed the presence of aberrant DNA methylation in GNAS imprinting cluster in NTD samples with low folate concentrations. Results from mouse models indicated that the establishment of GNAS imprinting was influenced by both maternal and paternal folate-deficient diets...
December 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29338857/obstetric-complications-after-frozen-versus-fresh-embryo-transfer-in-women-with-polycystic-ovary-syndrome-results-from-a-randomized-trial
#6
Bo Zhang, Daimin Wei, Richard S Legro, Yuhua Shi, Jing Li, Lin Zhang, Yan Hong, Gang Sun, Ting Zhang, Weiping Li, Zi-Jiang Chen
OBJECTIVE: To evaluate the effect of frozen embryo transfer on maternal and neonatal complications of singleton and twin pregnancies compared with fresh embryo transfer in women with polycystic ovary syndrome (PCOS). DESIGN: A secondary analysis of a multicenter, randomized, controlled trial comparing live birth after frozen vs. fresh embryo transfer (FreFro-PCOS). SETTING: Reproductive medicine centers. PATIENT(S): A total of 1,508 patients with a diagnosis of PCOS who were undergoing IVF were enrolled...
January 12, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29338139/occurrence-of-ovarian-follicular-dominance-during-stimulation-for-ivm-impacts-usable-blastocyst-yield
#7
Sergio Romero, Ricardo Pella, Francisco Escudero, Ygor Pérez, Mario García, Patricia Orihuela
OBJECTIVE: To evaluate the influence of ovarian follicular dominance on the outcome of oocyte in-vitro maturation. METHODS: This retrospective cohort study included 21 patients with polycystic ovaries or polycystic ovary syndrome (Rotterdam criteria, 2004) subjected to 24 in-vitro maturation (IVM) cycles between October 2015 and January 2017. Patients undergoing IVM received minimal gonadotropin stimulation starting on day 2 or 3 of the cycle; ovum pick-up typically occurred on days 6 to 8...
January 17, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29336996/follicular-flushing-in-patients-with-poor-ovarian-response-a-systematic-review-and-meta-analysis
#8
REVIEW
Kay Neumann, Georg Griesinger
To evaluate the effect of follicular flushing on clinical outcomes (primary outcome: mean number of COCs) in poor response IVF patients a systematic literature review and meta-analysis was performed. The bibliographic databases OvidMedline (includes Pubmed), Cochrane Library and Web of Science were searched electronically for randomized controlled trials (RCTs) comparing follicular flushing with no flushing. Three RCTs with a total of 210 patients could be included. The mean number of COC did not increase with flushing (weighted mean difference: -0...
December 29, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29336373/advances-of-long-noncoding-rnas-mediated-regulation-in-reproduction
#9
REVIEW
Kang-Sheng Liu, Tai-Ping Li, Hua Ton, Xiao-Dong Mao, Ya-Jun Chen
OBJECTIVE: Advances in genomics and molecular biology have led to the discovery of a large group of uncharacterized long noncoding RNAs (lncRNAs). Emerging evidence indicated that many lncRNAs function in multiple biological processes and its dysregulation often causes diseases. Recent studies suggested that almost all regulatory lncRNAs interact with biological macromolecules such as DNA, RNA, and protein. LncRNAs regulate gene expression mainly on three levels, including epigenetic modification, transcription, and posttranscription, through DNA methylation, histone modification, and chromatin remodeling...
January 20, 2018: Chinese Medical Journal
https://www.readbyqxmd.com/read/29334199/comparison-of-cabergoline-and-quinagolide-in-prevention-of-severe-ovarian-hyperstimulation-syndrome-among-patients-undergoing-intracytoplasmic-sperm-injection
#10
Robabeh Taheripanah, Mahshid Vasef, Marzieh Zamaniyan, Anahita Taheripanah
Background: The aim of the current study is to compare quinagolide with cabergoline in prevention of ovarian hyperstimulation syndrome (OHSS) among high risk women undergoing intracytoplasmic sperm injection (ICSI). MATERIALS AND METHODS: This randomized clinical trial study was performed from March 2015 to February 2017. One hundred and twenty six women undergoing ICSI who were at high risk of developing OHSS (having over 20 follicles of >12 mm), were randomized into two groups...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29334036/short-term-storage-of-the-oocytes-affects-the-ploidy-status-in-the-yellowtail-tetra-astyanax-altiparanae
#11
Matheus Pereira Dos Santos, Nivaldo Ferreira do Nascimento, George Shigueki Yasui, Nycolas Levy Pereira, Takafumi Fujimoto, José Augusto Senhorini, Laura Satiko Okada Nakaghi
In fish, many factors can affect reproduction during in vitro fertilization, therefore determination of the factors that affect affecting gamete quality is needed. However, few studies have focused on gamete quality and the ploidy status. This study was conducted to elucidate whether oocyte storage can affect ploidy status, survival, and embryo viability in the characid species Astyanax altiparanae. Oocytes were stored in Dulbecco's phosphate-buffered saline (PBS) at 26°C, then aliquots were fertilized immediately after extrusion (control) and also after 60, 120, 180, and 240 min of storage...
January 15, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/29333568/comparison-of-effects-of-thawing-entire-donor-sperm-vial-vs-partial-thawing-shaving-on-sperm-quality
#12
Micha Baum, Raoul Orvieto, Sapir Kon, Ronit Machtinger, Gil M Yerushalmi, Ariel Hourvitz
PURPOSE: Partial thawing of a vial of cryopreserved sperm (shaving) is sometimes applied as a measure to preserve sperm for further use, particularly in cases of very restricted sperm quantity. However, mechanical violence may disrupt the sperm-wall and lead to impaired in-vitro fertilization (IVF) outcomes. MATERIAL AND METHODS: In a retrospective case-control study at a tertiary, university-affiliated medical center, we compared the IVF/intracytoplasmic sperm injection (ICSI) outcomes of patients who used donor sperm following partial thawing (shaving) of the vial of cryopreserved sperm (n = 99) to a control group consisting of patients for whom the vial of sperm was completely thawed before use (n = 99)...
January 15, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29331495/role-of-bcl2l10-in-regulating-buffalo-bubalus-bubalis-oocyte-maturation
#13
Yu-Lin Huang, Huan-Jing Wang, Fu-Mei Chen, Xiu-Ling Zhao, Qiang Fu, Peng-Fei Zhang, Li-Ping Pu, Feng-Ling Huang, Yang-Qing Lu, Ming Zhang
It has been reported that BCL2L10 is abundantly and specifically expressed in adult human and mouse oocytes and played a very important role in oocytes maturation and early embryonic development. This study is to investigate the expression pattern of BCL2L10 in buffalo ovaries and its effect on the in vitro maturation of buffalo oocytes, so as to dissect mechanism of oocytes maturation and provide theoretical guidance for improvement of the in vitro maturation of buffalo oocytes. The results showed that BCL2L10 gene was enriched in ovary and the expression of BCL2L10 was oocyte specific and up-regulated during oocyte maturation...
December 30, 2017: Theriogenology
https://www.readbyqxmd.com/read/29325603/culture-conditions-affect-ca2-release-in-artificially-activated-mouse-and-human-oocytes
#14
Yuechao Lu, Davina Bonte, Minerva Ferrer-Buitrago, Mina Popovic, Jitesh Neupane, Margot Van der Jeught, Luc Leybaert, Petra De Sutter, Björn Heindryckx
Inconsistent fertilisation and pregnancy rates have been reported by different laboratories after application of ionomycin as a clinical method of assisted oocyte activation (AOA) to overcome fertilisation failure. Using both mouse and human oocytes, in the present study we investigated the effects of ionomycin and Ca2+ concentrations on the pattern of Ca2+ release and embryonic developmental potential. In the mouse, application of 5μM ionomycin in potassium simplex optimisation medium (KSOM) or 10µM ionomycin in Ca2+-free KSOM significantly reduced the Ca2+ flux and resulted in failure of blastocyst formation compared with 10μM ionomycin in KSOM...
January 12, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29325037/mediator-complex-component-med13-regulates-zygotic-genome-activation-and-is-required-for-postimplantation-development-in-the-mouse
#15
Yi-Liang Miao, Andrés Gambini, Yingpei Zhang, Elizabeth Padilla-Banks, Wendy N Jefferson, Miranda L Bernhardt, Weichun Huang, Leping Li, Carmen J Williams
Understanding factors that regulate zygotic genome activation (ZGA) is critical for determining how cells are reprogrammed to become totipotent or pluripotent. There is limited information regarding how this process occurs physiologically in early mammalian embryos. Here we identify a mediator complex subunit, MED13, as translated during mouse oocyte maturation and transcribed early from the zygotic genome. Knockdown and conditional knockout approaches demonstrate that MED13 is essential for ZGA in the mouse, in part by regulating expression of the embryo-specific chromatin remodeling complex, esBAF...
January 9, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29318868/embryo-development-until-blastocyst-stage-with-and-without-renewal-of-single-medium-on-day-3
#16
Fernando Peña, Rocío Dávalos, Adolfo Rechkemmer, Alvaro Ascenzo, Mauricio Gonzales
OBJECTIVE: This study aimed to assess the effects of using a single culture medium not renewed on day 3 on the development of the embryo up to the blastocyst stage. METHODS: The study was carried out in the Assisted Reproduction Laboratory of Clínica Miraflores in the period ranging from April to December of 2016. The study included 589 human embryos obtained from 82 couples submitted to IVF/ICSI with donor oocytes. The couples were randomly divided into two groups: group 1 (medium renewed on day 3) and group 2 (medium not renewed on day 3)...
January 10, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29315724/human-sperm-handling-in-intracytoplasmic-sperm-injection-processes-in-vitro-studies-on-mouse-oocyte-activation-embryo-development-competence-and-sperm-oxidation-reduction-potential
#17
S Roychoudhury, I Maldonado-Rosas, A Agarwal, S C Esteves, R Henkel, R Sharma
Polyvinylpyrrolidone (PVP) and hyaluronic acid (HA) are routinely used in handling spermatozoa for intracytoplasmic sperm injection (ICSI). As there are still concerns about possible adverse effects on the embryo, this study investigated sperm handling in a mouse ICSI model to (i) evaluate oocyte activation after injection of spermatozoa selected for rotational or linear motion in PVP; (ii) assess the effect of sperm selection in PVP, HA and medium on oocyte activation; (iii) examine the effects of PVP and HA on parthenogenetic oocyte activation and embryo development; and (iv) assess the oxidation-reduction potential (ORP) of spermatozoa exposed to PVP, HA or medium...
January 9, 2018: Andrologia
https://www.readbyqxmd.com/read/29315172/fertility-preservation-services-for-women-with-newly-diagnosed-cancer-a-national-assessment
#18
William D Winkelman, Mitchell P Rosen, Evelyn Mok-Lin
OBJECTIVE: To study the resources available for cancer patients once referred for fertility preservation. MATERIALS AND METHODS: Cross-sectional study clinics that are members of the Society for Assisted Reproductive Technology assess support for fertility preservation. RESULTS: Surveys were distributed to a total of 1135 members at 394 Society for Assisted Reproductive Technology clinics. Responses were received from 99 individuals representing a total of 84 unique clinics (21...
January 8, 2018: American Journal of Clinical Oncology
https://www.readbyqxmd.com/read/29310676/functional-role-of-akt-signaling-in-bovine-early-embryonic-development-potential-link-to-embryotrophic-actions-of-follistatin
#19
Mohamed Ashry, Sandeep K Rajput, Joseph K Folger, Jason G Knott, Nabil A Hemeida, Omaima M Kandil, Refaat S Ragab, George W Smith
BACKGROUND: TGF-β signaling pathways regulate several crucial processes in female reproduction. AKT is a non-SMAD signaling pathway regulated by TGF-β ligands essential for oocyte maturation and early embryonic development in the mouse, but its regulatory role in bovine early embryonic development is not well established. Previously, we demonstrated a stimulatory role for follistatin (a binding protein for specific members of TGF-β superfamily) in early bovine embryonic development...
January 8, 2018: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/29307115/bioinformatic-analyses-of-zona-pellucida-genes-in-vertebrates-and-their-expression-in-nile-tilapia
#20
Tianli Wu, Yunying Cheng, Zhilong Liu, Wenjing Tao, Shuqing Zheng, Deshou Wang
Zona pellucida (ZP) genes encode ZP glycoproteins which constitute the coat surrounding oocytes and early embryos. Genome-wide identification of ZP genes is still lacking in vertebrates, especially in fish species. Herein, we conducted bioinformatic analyses of the ZP genes of the Nile tilapia and other vertebrates. Totally 16, 9, 17, 27, 21, 20, 26, 19, 14,11, 24, 17, 9, 18, 8, 11, 9, 8, 5, and 4 ZP genes belonging to 5 subfamilies (ZPA, ZPB, ZPC, ZPD, and ZPAX) were found in the sea lamprey, elephant shark, coelacanth, spotted gar, zebrafish, medaka, stickleback, Nile tilapia, Amazon molly, platyfish, seahorse, Northern snakehead, cavefish, tetraodon, clawed frog, turtle, chicken, platypus, kangaroo rat, and human genomes, respectively...
January 6, 2018: Fish Physiology and Biochemistry
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