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https://www.readbyqxmd.com/read/28525345/in-vitro-production-of-sudanese-camel-camelus-dromedarius-embryos-from-epididymal-spermatozoa-and-follicular-oocytes-of-slaughtered-animals
#1
A E Abdelkhalek, Sh A Gabr, W A Khalil, Sh M Shamiah, L Pan, G Qin, M H Farouk
Application of assisted reproductive technology in camelidea, such as artificial insemination (AI) and embryo transfer, has been slow in comparison to that for other livestock species. In Egypt, there are few attempts to establish in vitro maturation (IVM) and fertilization (IVF) techniques in dromedary camel. The present study was carried out to produce Sudanese camel embryos using in vitro matured oocytes and epididymal spermatozoa. Dromedary camel ovaries were collected from abattoirs and then, the oocytes were aspirated from all the visible follicles on the ovarian surface (~2-8 mm in a diameter)...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28525327/in-vitro-fertilizing-potential-of-urethral-and-epididymal-spermatozoa-collected-from-domestic-cats-felis-catus
#2
S Prochowska, W Niżański
The aim of this study was to provide a comparative analysis of in vitro fertilizing potential of frozen-thawed urethral and epididymal feline spermatozoa. Both types of semen were collected from 7 cats and cryopreserved in liquid nitrogen. To perform in vitro fertilization, both urethral and epididymal samples from the same individual were thawed and spermatozoa were co-incubated with in vitro matured cat oocytes. Obtained embryos were cultured in vitro for 7 days in a commercial medium. Cleavage rate, morula rate and blastocyst rate were calculated...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28523429/kir-lilrb-and-their-ligands-genes-as-potential-biomarkers-in-recurrent-implantation-failure
#3
REVIEW
Izabela Nowak, Karolina Wilczyńska, Jacek R Wilczyński, Andrzej Malinowski, Paweł Radwan, Michał Radwan, Piotr Kuśnierczyk
Reproductive failure in humans is a very important social and economic problem, because nowadays women decide to conceive later in life and delay motherhood. Unfortunately, with increasing age they have less chance for natural fertilization and maintenance of pregnancy. Many of them need assisted reproductive technology. Approximately 10% of women after in vitro fertilization-embryo transfers experience recurrent implantation failure (RIF). Multiple factors may contribute to RIF, including oocyte and sperm quality, parental chromosomal anomalies, genetic or metabolic abnormalities of the embryo, poor uterine receptivity, immunological disturbances in the implantation site, and some gynecologic pathologies such as endometriosis, uterine fibroids, hydrosalpinx and endometrial polyps...
May 18, 2017: Archivum Immunologiae et Therapiae Experimentalis
https://www.readbyqxmd.com/read/28522611/long-terminal-repeats-power-evolution-of-genes-and-gene-expression-programs-in-mammalian-oocytes-and-zygotes
#4
Vedran Franke, Sravya Ganesh, Rosa Karlic, Radek Malik, Josef Pasulka, Filip Horvat, Maja Kuzman, Helena Fulka, Marketa Cernohorska, Jana Urbanova, Eliska Svobodova, Jun Ma, Yutaka Suzuki, Fugaku Aoki, Richard M Schultz, Kristian Vlahovicek, Petr Svoboda
Retrotransposons are 'copy-and-paste' insertional mutagens that substantially contribute to mammalian genome content. Retrotransposons often carry long terminal repeats (LTRs) for retrovirus-like reverse transcription and integration into the genome. We report an extraordinary impact of a group of LTRs from the mammalian endogenous retrovirus-related ERVL retrotransposon class on gene expression in the germline and beyond. In mouse, we identified >800 LTRs from ORR1, MT, MT2, and MLT families, which resemble mobile gene-remodeling platforms that supply promoters and first exons...
May 18, 2017: Genome Research
https://www.readbyqxmd.com/read/28520915/ovary-specific-depletion-of-the-nuclear-receptor-nr5a2-compromises-expansion-of-the-cumulus-oophorus-but-not-fertilization-by-icsi
#5
Kalyne Bertolin, Marie-Charlotte Meinsohn, João Suzuki, Jan Gossen, Kristina Schoonjans, Rajesha Duggavathi, Bruce D Murphy
The orphan nuclear receptor, liver receptor homolog-1 (Nr5a2) is widely expressed in mammalian tissues, its ovarian expression is restricted to granulosa cells of activated follicles. We employed the floxed Nr5a2 (Nr5a2f/f) mutant mouse line and two granulosa-specific Cre lines, Amhr2Cre and tgCyp19Cre, to develop two tissue- and time-specific Nr5a2 depletion models, Nr5a2Amhr2-/- and Nr5a2Cyp19-/-. In the Nr5a2Cyp19-/- ovaries, Nr5a2 was depleted in mural granulosa, but not cumulus cells. We induced follicular development in mutant and wild type (control, CON) mice with equine chorionic gonadotropin (eCG) followed 44h later treatment with human chorionic gonadotropin (hCG) to induce ovulation...
May 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28520897/alpha-linolenic-acid-protects-the-developmental-capacity-of-bovine-cumulus-oocyte-complexes-matured-under-lipotoxic-conditions-in-vitro
#6
W F A Marei, J De Bie, O Mohey-Elsaeed, E Wydooghe, P E J Bols, J L M R Leroy
Elevated concentrations of free fatty acids (FFAs), predominantly palmitic, stearic and oleic acids (PSO), exert detrimental effects on oocyte developmental competence. This study examined the effects of omega-3 alpha-linolenic acid (ALA) during in vitro oocyte maturation (IVM) in the presence of PSO on subsequent embryo development and quality, and the cellular mechanisms that might be involved. Bovine cumulus oocyte complexes (COCs) were supplemented during IVM with ALA (50 μM), PSO (425 μM), or PSO+ALA...
May 18, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28520729/top-quality-blastocyst-formation-rates-in-relation-to-progesterone-levels-on-the-day-of-oocyte-maturation-in-gnrh-antagonist-ivf-icsi-cycles
#7
V S Vanni, E Somigliana, M Reschini, L Pagliardini, E Marotta, S Faulisi, A Paffoni, P Vigano', W Vegetti, M Candiani, E Papaleo
Cycles with progesterone elevation during controlled ovarian stimulation (COS) for IVF/ICSI are commonly managed with a "freeze-all" strategy, due to a well-recognized detrimental effect of high progesterone levels on endometrial receptivity. However, also a detrimental effect of elevated progesterone on day-3 embryo quality has recently been found with regards to top quality embryo formation rate. Because blastocyst culture and cryopreservation are largely adopted, we deemed relevant to determine whether this detrimental effect is also seen on blastocyst quality on day 5-6...
2017: PloS One
https://www.readbyqxmd.com/read/28518112/functional-manipulation-of-maternal-gene-products-using-in-vitro-oocyte-maturation-in-zebrafish
#8
Elaine L Welch, Celeste C Eno, Sreelaja Nair, Robin E Lindeman, Francisco Pelegri
Cellular events that take place during the earliest stages of animal embryonic development are driven by maternally derived gene products deposited into the developing oocyte. Because these events rely on maternal products which typically act very soon after fertilization-that preexist inside the egg, standard approaches for expression and functional reduction involving the injection of reagents into the fertilized egg are typically ineffective. Instead, such manipulations must be performed during oogenesis, prior to or during the accumulation of maternal products...
April 22, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28518085/analysis-of-chromosome-segregation-histone-acetylation-and-spindle-morphology-in-horse-oocytes
#9
Federica Franciosi, Irene Tessaro, Rozenn Dalbies-Tran, Cecile Douet, Fabrice Reigner, Stefan Deleuze, Pascal Papillier, Ileana Miclea, Valentina Lodde, Alberto M Luciano, Ghylene Goudet
The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice...
May 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28513460/the-role-of-oxidative-stress-in-female-infertility-and-in-vitro-fertilization
#10
Joanna Wojsiat, Jerzy Korczyński, Marta Borowiecka, Halina Małgorzata Żbikowska
Infertility problem involves many couples of reproductive age. It has been estimated that in Poland 0.7-1.0 million pairs require treatment, while for more than half of them assisted reproduction is the only recommended and effective method. Infertility affects 13 to 15% of the world's population. A major concern is the age-related decline in female fertility even more that often a decision about pregnancy is taken at later age. Recent studies show that increased production of reactive oxygen species is an important factor in etiopathogenesis of pregnancy and affects female reproduction...
May 9, 2017: Postȩpy Higieny i Medycyny Doświadczalnej
https://www.readbyqxmd.com/read/28512855/insulin-like-growth-factor-i-enhances-the-developmental-competence-of-yak-embryos-by-modulating-aquaporin-3
#11
P Chen, Y Pan, Y Cui, Z Wen, P Liu, H He, Q Li, X Peng, T Zhao, S Yu
The objective of our present study was to determine the effects of insulin-like growth factor I (IGF-I) on the development of yak (Bos grunniens) embryos after cumulus-oocyte complex (COC) vitrification and warming followed by in vitro fertilization (IVF). In Experiment 1, the yak COCs underwent vitrification and then IVF. Embryos were incubated in synthetic oviductal fluid (SOF) supplemented with four concentrations (0, 50, 100 and 200 ng/ml) of IGF-I, while the yak COCs without vitrification or IGF-I supplementation acted as the control group; the BAX, BCL-2, AQP3mRNA and aquaporin 3 (AQP3) protein expression levels in the five groups of blastocysts were evaluated using quantitative real-time PCR and immunofluorescence analyses...
May 16, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28512846/melatonin-influences-the-sonic-hedgehog-signaling-pathway-in-porcine-cumulus-oocyte-complexes
#12
Sanghoon Lee, Jun-Xue Jin, Anukul Taweechaipaisankul, Geon A Kim, Curie Ahn, Byeong Chun Lee
Melatonin, which is synthesized in the pineal gland and peripheral reproductive organs, has antioxidant properties and regulates physiological processes. It is well known that melatonin affects in vitro maturation (IVM) of oocytes and embryonic development in many species. However, beneficial effects of melatonin on IVM have been explained mainly by indirect antioxidant effects and little information is available on the underlying mechanism by which melatonin directly acts on porcine cumulus oocyte complexes (COCs)...
May 17, 2017: Journal of Pineal Research
https://www.readbyqxmd.com/read/28511995/live-full-term-mouse-pups-from-oocytes-grown-and-matured-in-vitro-with-serum-substitutes
#13
Hideyuki H Motohashi, Ryoma Taniguchi, Junpei Sakamoto, Tadashi Sankai, Hidemi Kada
For in vitro growth and maturation of mouse oocytes (IVG-IVM), serum is added to media up to and including the stage of oocyte maturation; this subsequently supports oocytes through fertilization and early embryo development. However, problems may occur with sera, such as batch differences and issues of biosafety. The purpose of the present study was to determine the capacity for fertilization and pre- and post-implantation development of oocytes that underwent IVG-IVM with a serum substitute. Oocyte-granulosa cell complexes from preantral follicles were cultured in medium with either fetal bovine serum (FBS), Serum Substitute Supplement™ (SSS), or Knockout™ Serum Replacement (KSR) for 10days, and were then allowed to mature for 17 h...
May 13, 2017: Reproductive Biology
https://www.readbyqxmd.com/read/28505106/human-chorionic-gonadotropin-the-pregnancy-hormone-and-more
#14
REVIEW
Charalampos Theofanakis, Petros Drakakis, Alexandros Besharat, Dimitrios Loutradis
To thoroughly review the uses of human chorionic gonadotropin (hCG) related to the process of reproduction and also assess new, non-traditional theories. Review of the international literature and research studies. hCG and its receptor, LH/CGR, are expressed in numerous sites of the reproductive tract, both in gonadal and extra-goanadal tissues, promoting oocyte maturation, fertilization, implantation and early embryo development. Moreover, hCG seems to have a potential role as an anti-rejection agent in solid organ transplantation...
May 14, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28504820/using-propensity-score-matching-to-evaluate-the-effect-of-complementary-medicine-on-clinical-and-embryologic-outcomes-of-in-vitro-fertilization
#15
Anat Porat-Katz, Talia Eldar-Geva, Arik Kahane, Ora Paltiel
OBJECTIVE: To evaluate associations between using complementary medical therapies (CMTs) and embryologic and clinical outcomes of in vitro fertilization (IVF). METHODS: The present prospective questionnaire-based cohort study enrolled women aged 18-44 years undergoing their first, second, or third IVF cycle using their own oocytes at a large IVF clinic in Israel between February 1, 2013, and July 31, 2015. Clinical and embryologic data were obtained from patient records and patients completed a self-administered questionnaire that included details of any CMTs used to treat infertility...
May 15, 2017: International Journal of Gynaecology and Obstetrics
https://www.readbyqxmd.com/read/28502309/effects-of-vitrification-of-cumulus-enclosed-porcine-oocytes-at-the-germinal-vesicle-stage-on-cumulus-expansion-nuclear-progression-and-cytoplasmic-maturation
#16
Ruth Appeltant, Tamás Somfai, Elisa C S Santos, Thanh Quang Dang-Nguyen, Takashi Nagai, Kazuhiro Kikuchi
Although offspring have been produced from porcine oocytes vitrified at the germinal vesicle (GV) stage, the rate of embryo development remains low. In the present study, nuclear morphology and progression, cumulus expansion, transzonal projections (TZPs), ATP and glutathione (GSH) levels were compared between vitrified cumulus-oocyte complexes (COCs) and control COCs (no cryoprotectant treatment and no cooling), as well as a toxicity control (no cooling). Vitrification was performed with 17.5% (v/v) ethylene glycol and 17...
May 15, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28501428/management-of-ovarian-stimulation-for-ivf-narrative-review-of-evidence-provided-for-world-health-organization-guidance
#17
REVIEW
Cindy Farquhar, Jane Marjoribanks, Julie Brown, Bart C J M Fauser, Anne Lethaby, Selma Mourad, Robert Rebar, Marian Showell, Sheryl van der Poel
In this paper, a review of evidence provided to the World Health Organization (WHO) guideline development, who prepare global guidance on the management of ovarian stimulation for women undergoing IVF, is presented. The purpose of ovarian stimulation is to facilitate retrieval of multiple oocytes during a single IVF cycle. Availability of multiple oocytes compensates for inefficiencies in subsequent stages of the cycle, which include oocyte maturation, IVF, embryo culture, embryo transfer, and implantation...
April 26, 2017: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/28501366/reproductive-outcomes-after-a-single-dose-of-gonadotropin-releasing-hormone-agonist-compared-with-human-chorionic-gonadotropin-for-the-induction-of-final-oocyte-maturation-in-hyper-responder-women-aged-35-40%C3%A2-years
#18
Samer Tannus, Rola Turki, Yoni Cohen, Weon-Young Son, Tal Shavit, Michael Haim Dahan
OBJECTIVE: To investigate the reproductive outcomes after the use of GnRH agonist (GnRHa) compared with hCG for the induction of final oocyte maturation in GnRH antagonist cycles performed in hyper-responder women aged 35-40 years. DESIGN: Retrospective study. SETTING: Academic fertility center. PATIENT(S): Two hundred seventy-two hyper-responder women aged 35-40 years who underwent controlled ovarian stimulation under GnRH antagonist suppression were included...
May 10, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28501365/why-natural-killer-cells-are-not-enough-a-further-understanding-of-killer-immunoglobulin-like-receptor-and-human-leukocyte-antigen
#19
REVIEW
Diana Alecsandru, Juan A García-Velasco
The immune system's role in recurrent reproductive failure is a controversial issue in assisted reproduction. Most studies into immune system implication in reproduction have focused on finding markers of peripheral blood and less on the uterine environment. Peripheral blood natural killer cells have become an "immune study core" for women with recurrent miscarriage or recurrent implantation failure, based on the mistaken notion that they cause reproductive failure by killing or "rejecting" the embryo. Maternal-fetal tolerance begins at the uterine level, so successful adaptation to the fetus occurs after a complicated process...
May 10, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28500053/manipulating-the-mitochondrial-genome-to-enhance-cattle-embryo-development
#20
Kanokwan Srirattana, Justin C St John
The mixing of mitochondrial DNA (mtDNA) from the donor cell and the recipient oocyte in embryos and offspring derived from somatic cell nuclear transfer (SCNT) compromises genetic integrity and affects embryo development. We set out to generate SCNT embryos that inherited their mtDNA from the recipient oocyte only, as is the case following natural conception. Whilst SCNT blastocysts produced from Holstein (Bos Taurus) fibroblasts depleted of their mtDNA and oocytes derived from Angus (Bos Taurus) cattle possessed oocyte mtDNA only, the co-existence of donor cell and oocyte mtDNA resulted in blastocysts derived from non-depleted cells...
May 12, 2017: G3: Genes—Genomes—Genetics
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