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https://www.readbyqxmd.com/read/29780295/successful-in-vitro-fertilization-and-embryo-transfer-after-transplantation-of-cryopreserved-ovarian-tissue-report-of-the-first-korean-case
#1
Jung Ryeol Lee, Dayong Lee, Soohyeon Park, Eun Chan Paik, Seul Ki Kim, Byung Chul Jee, Chang Suk Suh, Seok Hyun Kim
For patients at risk of premature ovarian failure with cancer treatment, it is an important option to re-implant the ovarian tissue (OT) after cryopreservation to preserve endocrine function and fertility. With this technique, about 30% of pregnancy success rate and about 90 live births have been reported to date. However, there has been no case report of successful in vitro fertilization (IVF) and embryo transfer (ET) with oocytes collected from transplanted cryopreserved OT in Korea. We report a 30-year old woman with rectal cancer who underwent IVF and ET after cryopreserved OT thawing and re-implantation...
May 21, 2018: Journal of Korean Medical Science
https://www.readbyqxmd.com/read/29779474/the-gametotoxic-effects-of-the-endometrioma-content-insights-from-a-parthenogenetic-human-model
#2
Alessio Paffoni, Valentina Bolis, Stefania Ferrari, Laura Benaglia, Paolo Vercellini, Edgardo Somigliana
The present randomized controlled in vitro study was designed to evaluate the effects of the exposure of human cryopreserved oocytes to endometriotic fluid. Twenty-three women aged 36 (4) years donated a total of 147 vitrified supernumerary metaphase II oocytes. Warmed oocytes were randomly assigned to exposure to endometriotic fluid or unexposed control. Thereafter, oocytes were parthenogenetically activated and cultured for up to 5 days. The rate of activation on day 1 and the developmental rates on days 3 and 5 did not significantly differ between the 2 groups...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29779472/effect-of-body-weight-on-metabolic-hormones-and-fatty-acid-metabolism-in-follicular-fluid-of-women-undergoing-in-vitro-fertilization-a-pilot-study
#3
Laurice Bou Nemer, Haolin Shi, Bruce Richard Carr, Ruth Ann Word, Orhan Bukulmez
INTRODUCTION: Obesity is an epidemic affecting more than one-third of adults in the United States. Obese women experience decreased fertility, explained in part by oocyte quality. Since follicular fluid (FF) provides an important microenvironment for oocyte growth, we sought to evaluate the effect of increased body weight on FF levels of 11 metabolic hormones and fatty acid metabolism. METHODS: The FF was collected from 25 women (10 normal weight, 10 overweight, and 5 obese) with diminished ovarian reserve undergoing in vitro fertilization (IVF) following a minimal stimulation protocol...
January 1, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/29778922/interval-of-gonadotropin-administration-for-in-vitro-embryo-production-from-oocytes-collected-from-holstein-calves-between-2-and-6-months-of-age-by-repeated-laparoscopy
#4
Hernan Baldassarre, Luke Currin, Laura Michalovic, Anne-Marie Bellefleur, Karina Gutierrez, Rafael G Mondadori, Werner G Glanzner, Yasmin Schuermann, Rodrigo C Bohrer, Naomi Dicks, Rosalba Lopez, François-Xavier Grand, Christian Vigneault, Patrick Blondin, Jim Gourdon, Vilceu Bordignon
Laparoscopic Ovum Pick-Up (LOPU) in calves followed by in vitro embryo production (IVEP) and transfer (ET) into adult recipients has great potential for accelerated genetic gain through shortening of the generation interval. In this study, 11 Holstein calves were subjected to up to six LOPU procedures between the ages of 2-6 months at 2-3 weeks interval. In all cases, the animals received a CIDR 5 days prior to LOPU and were gonadotropin-stimulated starting at 72 h before LOPU using one of three protocols that were rotated twice among the animals during the study...
May 17, 2018: Theriogenology
https://www.readbyqxmd.com/read/29778554/cumulative-live-birth-rates-after-ivf-in-patients-with-polycystic-ovaries-phenotype-matters
#5
Michel De Vos, Stéphanie Pareyn, Panagiotis Drakopoulos, José M Raimundo, Ellen Anckaert, Samuel Santos-Ribeiro, Nikolaos P Polyzos, Herman Tournaye, Christophe Blockeel
RESEARCH QUESTION: Do cumulative live birth rates (CLBR) vary among women with different polycystic ovary syndrome (PCOS) phenotypes who undergo IVF/intracytoplasmic sperm injection (ICSI) treatment? DESIGN: In this retrospective cohort study, data from 567 patients undergoing an assisted reproductive technology (ART) cycle between January 2010 and December 2015 were collected. Demographical traits, cycle characteristics and clinical and laboratory data were analysed...
May 7, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29778392/ultrasound-guided-follicle-aspiration-at-time-of-laparotomy-in-a-patient-with-mayer-rokitansky-k%C3%A3-ster-hauser-syndrome
#6
Ashley M Eskew, Darcy E Broughton, Maureen B Schulte, Kenan R Omurtag, Randall R Odem
OBJECTIVE: To demonstrate a unique case of direct ultrasound-guided follicle aspiration at time of a laparotomy in a patient with Mayer-Rokitansky-Küster-Hauser Syndrome. DESIGN: Video presentation. SETTING: Academic fertility center PATIENT(S): A 32-year-old gravida 0 with Mayer-Rokitansky-Küster-Hauser Syndrome and bilateral ovarian masses presented as a fertility preservation consult from gynecologic oncology due to the possibility of bilateral oophorectomy...
May 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29775237/yolk-accumulation-in-an-insect-oocyte
#7
Isabela Ramos
Oocytes are remarkable cells in the matter of accumulating macronutrients. Oviparous animals have evolved to produce germline cells that not only enter meiosis to generate a gamete, but also differentiate into a giant cell designed to support embryo growth. To accomplish that, an oocyte typically grows up to 5,000x its original size by accumulating macromolecules, which are collectively referred to as yolk. This article is protected by copyright. All rights reserved.
May 18, 2018: Molecular Reproduction and Development
https://www.readbyqxmd.com/read/29774457/vitamin-d-as-a-follicular-marker-of-human-oocyte-quality-and-a-serum-marker-of-in-vitro-fertilization-outcome
#8
Przemysław Ciepiela, Antoni J Dulęba, Ewelina Kowaleczko, Kornel Chełstowski, Rafał Kurzawa
PURPOSE: This study investigated the relationship between the vitamin D [25(OH)D] level in individual follicles and oocyte developmental competence. METHODS: A prospective cohort study in a private infertility center. Infertile women (N = 198) scheduled for intracytoplasmic sperm injection (ICSI) and a single embryo transfer (SET) provided serum samples and 322 follicular fluid (FF) specimens, each from a single follicle on the day of oocyte retrieval. RESULTS: FFs corresponding to successfully fertilized oocytes (following ICSI) contained significantly lower 25(OH)D level compared with those that were not fertilized (28...
May 17, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/29770509/molecular-characteristics-of-granulosa-and-cumulus-cells-and-oocyte-competence-in-nelore-cows-with-low-and-high-numbers-of-antral-follicles
#9
C O Rosa, Lsr Marinho, Pra da Rosa, M P De Cesaro, P A Lunardelli, K C Silva-Santos, A C Basso, V Bordignon, M M Seneda
The aims of this study were to investigate whether the number of antral follicles (AF) in the ovaries of Nelore cows is influenced with the developmental competence of oocytes to reach the blastocyst stage and to quantify the mRNA abundance of genes associated with folliculogenesis and oogenesis in granulosa and cumulus cells. A total of 168 cows were distributed into two experimental groups according to the number of AF, low (≤31) and high AF (≥92), which were determined based on the mean number of AF (61...
May 16, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29769451/effect-of-ferulic-acid-supplementation-on-the-developmental-competence-of-porcine-embryos-during-in-vitro-maturation
#10
Fuminori Tanihara, Maki Hirata, Nguyen Thi Nhien, Takayuki Hirano, Toshiki Kunihara, Takeshige Otoi
The value of laboratory and genetically-modified pigs is becoming increasingly clear; however, their in vitro development remains inefficient. Trans-ferulic acid (trans-FA) is an aromatic compound that is abundant in plant cell walls, and which exhibits antioxidant effects in vitro. Trans-FA is known to improve sperm viability and motility; however, its effects on porcine oocytes are unknown. Our aim was to investigate the effects of trans-FA supplementation during in vitro maturation on the meiotic and developmental competence of porcine oocytes...
May 16, 2018: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29768947/linking-back-to-back-stimulation-cycles-with-oral-contraceptives-or-progestins-in-women-undergoing-embryo-accumulation-for-preimplantation-genetic-testing-a-retrospective-study
#11
Jorge Rodriguez-Purata, Marta Devesa, Mónica Parriego, Clara Pardos, Ignacio Rodriguez, Nikolaos P Polyzos, Francisca Martínez, Buenaventura Coroleu
This retrospective study was carried out to determine which strategy is associated with improved outcomes in two back-to-back cycles when undergoing embryo accumulation. Eighty patients with two stimulation cycles performed with <45 days between retrievals between Jan'16-Mar'17 were included. Patients were segregated according to the strategy used to link stimulations: spontaneous menses (SM), vaginal micronized progesterone (VMP) or oral contraceptive pills (OCP). Main outcome measure was oocytes retrieved...
May 17, 2018: Gynecological Endocrinology
https://www.readbyqxmd.com/read/29768406/silencing-of-rpatg6-impaired-the-yolk-accumulation-and-the-biogenesis-of-the-yolk-organelles-in-the-insect-vector-r-prolixus
#12
Priscila H Vieira, Larissa Bomfim, Georgia C Atella, Hatisaburo Masuda, Isabela Ramos
In oviparous animals, the egg yolk is synthesized by the mother in a major metabolic challenge, where the different yolk components are secreted to the hemolymph and delivered to the oocytes mostly by endocytosis. The yolk macromolecules are then stored in a wide range of endocytic-originated vesicles which are collectively referred to as yolk organelles and occupy most of the mature oocytes cytoplasm. After fertilization, the contents of these organelles are degraded in a regulated manner to supply the embryo cells with fundamental molecules for de novo synthesis...
May 16, 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29768195/reprogramming-of-chromatin-accessibility-in-somatic-cell-nuclear-transfer-is-dna-replication-independent
#13
Mohamed Nadhir Djekidel, Azusa Inoue, Shogo Matoba, Tsukasa Suzuki, Chunxia Zhang, Falong Lu, Lan Jiang, Yi Zhang
Mammalian oocytes have the ability to reset the transcriptional program of differentiated somatic cells into that of totipotent embryos through somatic cell nuclear transfer (SCNT). However, the mechanisms underlying SCNT-mediated reprogramming are largely unknown. To understand the mechanisms governing chromatin reprogramming during SCNT, we profiled DNase I hypersensitive sites (DHSs) in donor cumulus cells and one-cell stage SCNT embryos. To our surprise, the chromatin accessibility landscape of the donor cells is drastically changed to recapitulate that of the in vitro fertilization (IVF)-derived zygotes within 12 hr...
May 15, 2018: Cell Reports
https://www.readbyqxmd.com/read/29766148/endogenous-luteinizing-hormone-concentration-and-ivf-outcome-during-ovarian-stimulation-in-fixed-versus-flexible-gnrh-antagonist-protocols-an-rct
#14
Raffaella Depalo, Paolo Trerotoli, Annarosa Chincoli, Margherita Patrizia Vacca, Giuseppina Lamanna, Ettore Cicinelli
Background: Luteinizing hormone (LH) is essential for normal follicular development and oocyte maturation. In particular, fluctuations of LH during the follicular phase have a significant impact on morphological and functional changes of the oocyte and determine its meiotic status and ability to be fertilized. Objective: This prospective randomized controlled trial examined effects of endogenous follicular phase LH levels on oocyte maturity and IVF outcomes in fixed vs...
March 2018: International Journal of Reproductive Biomedicine (Yazd, Iran)
https://www.readbyqxmd.com/read/29762687/glycine-treatment-enhances-developmental-potential-of-porcine-oocytes-and-early-embryos-by-inhibiting-apoptosis
#15
Suo Li, Qing Guo, Yu-Meng Wang, Zi-Yue Li, Jin-Dan Kang, Xi-Jun Yin, Xin Zheng
Glycine, a component of glutathione (GSH), plays an important role in protection from reactive oxygen species (ROS) and inhibition of apoptosis. The aim of this study was to determine the effect of glycine on in vitro maturation (IVM) of porcine oocytes and their developmental competence after parthenogenetic activation (PA). We examined nuclear maturation, ROS levels, apoptosis, mitochondrial membrane potential (ΔΨm), and ATP concentration, as well as the expression of several genes related to oocyte maturation and development...
May 12, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29758296/isolation-of-the-sex-determining-gene-sex-lethal-sxl-in-penaeus-litopenaeus-vannamei-boone-1931-and-characterization-of-its-embryogenic-gametogenic-and-tissue-specific-expression
#16
Itzia López-Cuadros, Alejandra García-Gasca, Gracia Gomez-Anduro, Cristina Escobedo-Fregoso, Raúl A Llera-Herrera, Ana M Ibarra
The Pacific white shrimp Penaeus vannamei is the most cultured shrimp species around the world. Because females grow larger than males, the culture of 'only females' is of great interest, but knowledge on sex determination and differentiation is required for producing only females. In an effort to obtain information associated with reproduction in P. vannamei, transcriptomic data from female gonads was generated, and partial sequences of a transcript were identified as Sex-lethal (Sxl). Its characterization indicated that, differently from other penaeids in which this gene has been isolated, there are six isoforms of the Sxl transcript in P...
May 11, 2018: Gene
https://www.readbyqxmd.com/read/29757594/transport-ivf-icsi-results-of-a-25-year-experience
#17
Fernanda G E Raffo, Jorge Blaquier
OBJECTIVE: This report presents a summary of the outcomes achieved at ART center FERTILAB in Buenos Aires, Argentina, with transport IVF-ICSI for 25 years (1990-2014). METHODS: This report included all patients submitted to oocyte retrieval for IVF-ICSI whose oocytes were transported from sites of aspiration located 0.5-58 kilometers away from the central laboratory. The numeric data herein reported were taken from annual reports submitted by our institution (Fertilab) and, for purposes of comparison, by all Argentinian centers to the Latin American Registry of Assisted Reproduction (RLA) within the same time period...
May 14, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29757581/transmyometrial-embryo-transfer-as-a-useful-method-to-overcome-difficult-embryo-transfers-a-single-center-retrospective-study
#18
Janisse Ferreri Dos Anjos, Emma Gabriela Portillo Osorio, Joana Peñarrubia, Ester Vidal, Francisco Fábregues Gasol
OBJECTIVE: Pregnancy after an embryo transfer depends largely on embryo quality, endometrial receptivity, and the technique used in the embryo transfer. Embryo transfers have been reported as inevitably traumatic and difficult for 5-7% of patients in assisted reproduction treatment. In these cases, transmyometrial embryo transfer should be considered as a suitable method to overcome difficult embryo transfers. The aim of this study was to report our experience with this technique and analyze its causes, results and complications...
May 14, 2018: JBRA Assisted Reproduction
https://www.readbyqxmd.com/read/29755732/recent-advances-in-understanding-oogenesis-interactions-with-the-cytoskeleton-microtubule-organization-and-meiotic-spindle-assembly-in-oocytes
#19
REVIEW
Florence L Marlow
Maternal control of development begins with production of the oocyte during oogenesis. All of the factors necessary to complete oocyte maturation, meiosis, fertilization, and early development are produced in the transcriptionally active early oocyte. Active transcription of the maternal genome is a mechanism to ensure that the oocyte and development of the early embryo begin with all of the factors needed for successful embryonic development. To achieve the maximum maternal store, only one functional cell is produced from the meiotic divisions that produce the oocyte...
2018: F1000Research
https://www.readbyqxmd.com/read/29751329/determination-of-metals-and-pharmaceutical-compounds-released-in-hospital-wastewater-from-toluca-mexico-and-evaluation-of-their-toxic-impact
#20
Itzayana Pérez-Alvarez, Hariz Islas-Flores, Leobardo Manuel Gómez-Oliván, Damià Barceló, Miren López De Alda, Sandra Pérez Solsona, Livier Sánchez-Aceves, Nely SanJuan-Reyes, Marcela Galar-Martínez
Due to the activities inherent to medical care units, the hospital effluent released contains diverse contaminants such as tensoactives, disinfectants, metals, pharmaceutical products and chemical reagents, which are potentially toxic to the environment since they receive no treatment or are not effectively removed by such treatment before entering the drain. They are incorporated into municipal wastewater, eventually entering water bodies where they can have harmful effects on organisms and can result in ecological damage...
May 8, 2018: Environmental Pollution
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