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https://www.readbyqxmd.com/read/29223528/glp-1-receptor-agonists-downregulate-aberrant-gnt-iii-expression-in-alzheimer-s-disease-models-through-the-akt-gsk-3%C3%AE-%C3%AE-catenin-signaling
#1
Ying Wang, Song Chen, Zheng Xu, Suting Chen, Wenbing Yao, Xiangdong Gao
Alterations of glycoprotein glycans contribute to a wide variety of diseases. Bisecting N-acetylglucosamine (GlcNAc) levels increased in the cerebrospinal fluid of most Alzheimer's disease (AD) patients, and the mRNA levels of N-acetylglucosaminyltransferase III (GnT-III), a glycosyltransferase responsible for synthesizing a bisecting GlcNAc residue, were found highly expressed in the brains of AD patients. In our previous studies, glucagon-like peptide-1 (GLP-1) and its mimetics showed neuroprotective effects...
December 6, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/29220650/the-haystack-is-full-of-needles-technology-rescues-sugars
#2
Richard D Cummings
The recent paper by Stadlmann et al. (2017) provides a novel algorithm for glycoproteomics in which complex glycopeptides can be identified in complex mixtures to aid in characterizing both the site of glycosylation and the glycan structure.
December 7, 2017: Molecular Cell
https://www.readbyqxmd.com/read/29219305/brucellosis-improved-diagnostics-and-vaccine-insights-from-synthetic-glycans
#3
David R Bundle, John McGiven
Brucellosis is a serious zoonotic bacterial disease that is ranked by the World Health Organization among the top seven "neglected zoonoses" that threaten human health and cause poverty. It is a costly, highly contagious disease that affects ruminants, cattle, sheep, goats, and other productive animals such as pigs. Symptoms include abortions, infertility, decreased milk production, weight loss, and lameness. Brucellosis is also the most common bacterial disease that is transmitted from animals to humans, with approximately 500 000 new human cases each year...
December 8, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/29217428/clinical-significance-of-galnacylated-glycans-in-cholangiocarcinoma-values-for-diagnosis-and-prognosis
#4
Waraporn Saentaweesuk, Atit Silsirivanit, Kulthida Vaeteewoottacharn, Kanlayanee Sawanyawisuth, Chawalit Pairojkul, Ubon Cha'on, Somsiri Indramanee, Somchai Pinlaor, Thidarut Boonmars, Norie Araki, Chaisiri Wongkham
Cancer cells exhibited the aberrant cancer-associated glycans that are potential biomarkers for diagnosis and monitoring of the cancer. In this study, Sophora japonica agglutinin (SJA) was used to detect SJA-specific N-acetylgalactosamine-associated glycans (SNAG) in liver tissues and sera from cholangiocarcinoma (CCA) patients. Whether SNAG could be the diagnostic and prognostic markers for CCA was evaluated. SJA-histochemistry revealed that SNAG was undetec2 in normal bile ducts but was highly expressed in hyperplastic/dysplastic bile ducts and CCA...
December 4, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29217182/mass-spectrometry-characterization-for-n-glycosylation-of-immunoglobulin-y-from-hen-egg-yolk
#5
Long Sheng, Zhenjiao He, Yaping Liu, Meihu Ma, Zhaoxia Cai
Immunoglobulin Y (IgY) is a new therapeutic antibody that exists in hen egg yolk. It is a glycoprotein, not much is known about its N-glycan structures, site occupancy and site-specific N-glycosylation. In this study, purified protein from hen egg yolk was identified as IgY based on SDS-PAGE and MALDI-TOF/TOF MS. N-glycan was released from IgY using peptide-N4-(N-acetyl-beta-glucosaminyl) asparagine-amidase treatment, and the molecular weight of IgY was calculated using the difference between the molecular weight of IgY and deglycosylated IgY...
December 4, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29215790/inhibition-of-fucosylation-in-human-invasive-ductal-carcinoma-reduces-e-selectin-ligand-expression-cell-proliferation-and-erk1-2-and-p38-mapk-activation
#6
M A Carrascal, M Silva, J S Ramalho, C Pen, M Martins, C Pascoal, C Amaral, I Serrano, M J Oliveira, R Sackstein, P A Videira
Breast cancer tissue overexpresses fucosylated glycans, such as sialyl-Lewis X/A (sLeX/A ), and α-1,3/4-fucosyltransferases in relation to increased disease progression and metastasis. These glycans in tumor circulating cells mediate binding to vascular E-selectin, initiating tumor extravasation. However, their role(s) in breast carcinogenesis is still unknown. Here, we aimed to define the contribution of the fucosylated structures, including sLeX/A , to cell adhesion, cell signaling and proliferation in invasive ductal carcinomas (IDC), the most frequent type of breast cancer...
December 7, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/29214176/human-gut-microbiota-associated-with-obesity-in-chinese-children-and-adolescents
#7
Ya-Ping Hou, Qing-Qing He, Hai-Mei Ouyang, Hai-Shan Peng, Qun Wang, Jie Li, Xiao-Fei Lv, Yi-Nan Zheng, Shao-Chuan Li, Hai-Liang Liu, Ai-Hua Yin
Objective: To investigate the gut microbiota differences of obese children compared with the control healthy cohort to result in further understanding of the mechanism of obesity development. Methods: We evaluated the 16S rRNA gene, the enterotypes, and quantity of the gut microbiota among obese children and the control cohort and learned the differences of the gut microbiota during the process of weight reduction in obese children. Results: In the present study, we learned that the gut microbiota composition was significantly different between obese children and the healthy cohort...
2017: BioMed Research International
https://www.readbyqxmd.com/read/29212851/omics-of-bifidobacteria-research-and-insights-into-their-health-promoting-activities
#8
REVIEW
Francesca Bottacini, Douwe van Sinderen, Marco Ventura
Members of the genus Bifidobacterium include gut commensals that are particularly abundant among the microbial communities residing in the gut of healthy breast-fed infants, where their presence has been linked to many beneficial host effects. Next-generation DNA sequencing and comparative and functional genome methodologies have been shown to be particularly useful in exploring the diversity of this genus. These combined approaches have allowed the identification of genetic features related to bifidobacterial establishment in the gut, involving host-microbe as well as microbe-microbe interactions...
December 6, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29212795/degradation-pathway-of-plant-complex-type-n-glycans-identification-and-characterization-of-a-key-%C3%AE-1-3-fucosidase-from-glycoside-hydrolase-family-29
#9
Shun Kato, Megumi Hayashi, Mai Kitagawa, Hiroyuki Kajiura, Megumi Maeda, Yoshinobu Kimura, Kiyohiko Igarashi, Masahiro Kasahara, Takeshi Ishimizu
Plant complex-type N -glycans are characterized by the presence of α1,3-linked fucose towards the proximal N -acetylglucosamine residue and β1,2-linked xylose towards the β-mannose residue. These glycans are ultimately degraded by the activity of several glycoside hydrolases. However, the degradation pathway of plant complex-type N -glycans have not been entirely elucidated because the gene encoding α1,3-fucosidase, a glycoside hydrolase acting on plant complex-type N -glycans, has not yet been identified, and its substrate specificity remains to be determined...
December 6, 2017: Biochemical Journal
https://www.readbyqxmd.com/read/29212317/targeted-measurements-of-o-and-n-glycopeptides-show-that-proteins-in-hdl-particles-are-enriched-with-specific-glycosylation-compared-to-plasma
#10
Muchena J Kailemia, Wanghui Wei, Khoa Nguyen, Elizabeth Beals, Lisa Sawrey-Kubicek, Christopher Rhodes, Chenghao Zhu, Romina Sacchi, Angela M Zivkovic, Carlito B Lebrilla
High density lipoprotein (HDL) particles are believed to be protective due to their inverse correlation with the prevalence of cardiovascular diseases. However, recent studies show that in some conditions such as heart disease and diabetes, HDL particles can become dysfunctional. Great attention has been directed toward HDL particle composition because the relative abundances of HDL constituents determine HDL's functional properties. A key factor to consider when studying the structure and composition of plasma particles is the protein glycosylation...
December 7, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29210993/aberrant-n-glycosylation-profile-of-serum-immunoglobulins-is-a-diagnostic-biomarker-of-urothelial-carcinomas
#11
Toshikazu Tanaka, Tohru Yoneyama, Daisuke Noro, Kengo Imanishi, Yuta Kojima, Shingo Hatakeyama, Yuki Tobisawa, Kazuyuki Mori, Hayato Yamamoto, Atsushi Imai, Takahiro Yoneyama, Yasuhiro Hashimoto, Takuya Koie, Masakazu Tanaka, Shin-Ichiro Nishimura, Shizuka Kurauchi, Ippei Takahashi, Chikara Ohyama
The aim of this study to determine whether the aberrant N-glycosylated serum immunoglobulins (Igs) can be applied as a diagnostic marker of urothelial carcinoma (UC). Between 2009 and 2016, we randomly obtained serum available from 237 UC and also 96 prostate cancer as other cancer controls from our serum bank and also obtained-from 339 healthy volunteers (HV)-controls obtained from community-dwelling volunteers in Iwaki Health Promotion Project. A total of 32 types of N-glycan levels on Igs were determined by high-throughput N-glycomics and analyzed by multivariable discriminant analysis...
December 6, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29210428/lectin-gated-and-glycan-functionalized-mesoporous-silica-nanocontainers-for-targeting-cancer-cells-overexpressing-lewis-x-antigen
#12
R Bhat, I García, E Aznar, B Arnaiz, M C Martínez-Bisbal, L M Liz-Marzán, S Penadés, R Martínez-Máñez
Gated mesoporous silica nanoparticles can deliver payload upon the application of a predefined stimulus, and therefore are promising drug delivery systems. Despite their important role, relatively low emphasis has been placed on the design of gating systems that actively target carbohydrate tumor cell membrane receptors. We describe herein a new Lewis X (Lex) antigen-targeted delivery system comprising mesoporous silica nanoparticles (MSNs) loaded with ATTO 430LS dye, functionalized with a Lex derivative (1) and capped with a fucose-specific carbohydrate-binding protein (Aleuria aurantia lectin (AAL))...
December 6, 2017: Nanoscale
https://www.readbyqxmd.com/read/29207682/over-forty-years-of-bladder-cancer-glycobiology-where-do-glycans-stand-facing-precision-oncology
#13
REVIEW
Rita Azevedo, Andreia Peixoto, Cristiana Gaiteiro, Elisabete Fernandes, Manuel Neves, Luís Lima, Lúcio Lara Santos, José Alexandre Ferreira
The high molecular heterogeneity of bladder tumours is responsible for significant variations in disease course, as well as elevated recurrence and progression rates, thereby hampering the introduction of more effective targeted therapeutics. The implementation of precision oncology settings supported by robust molecular models for individualization of patient management is warranted. This effort requires a comprehensive integration of large sets of panomics data that is yet to be fully achieved. Contributing to this goal, over 40 years of bladder cancer glycobiology have disclosed a plethora of cancer-specific glycans and glycoconjugates (glycoproteins, glycolipids, proteoglycans) accompanying disease progressions and dissemination...
October 31, 2017: Oncotarget
https://www.readbyqxmd.com/read/29207246/multiple-reaction-monitoring-for-the-quantitation-of-serum-protein-glycosylation-profiles-application-to-ovarian-cancer
#14
Suzanne Miyamoto, Carol D Stroble, Sandra Taylor, Qiuting Hong, Carlito B Lebrilla, Gary S Leiserowitz, Kyoungmi Kim, L Renee Ruhaak
Protein glycosylation fingerprints are widely recognized as potential markers for disease states and indeed differential glycosylation has been identified in multiple types of autoimmune diseases and several types of cancer. However, releasing the glycans leave the glycoproteins unknown, therefore there exists a need for high-throughput methods that allow quantification of site- and protein- specific glycosylation patterns from complex biological mixtures. In this study, a targeted multiple reaction monitoring (MRM)-based method for the protein- and site- specific quantitation involving serum proteins immunoglobulins A, G and M, alpha-1-antitrypsin, transferrin, alpha-2-macroglobulin, haptoglobin, alpha-1-acid glycoprotein and complement C3 was developed...
December 5, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29206917/tunicamycin-induced-er-stress-in-breast-cancer-cells-neither-expresses-grp78-on-the-surface-nor-secretes-it-into-the-media
#15
Jesús E Serrano-Negrón, Zhenbo Zhang, Andrea P Rivera-Ruiz, Aditi Banerjee, Eva C Romero-Nutz, Neysharie Sánchez-Torres, Krishna Baksi, Dipak K Banerjee
GRP78 (an Mr 78 kDa calcium dependent glucose binding protein) located in ER lumen. It functions as ER chaperone and translocates proteins for glycosylation at the asparagine residue present in the sequon Asn-X-Ser/Thr. Paraffin sections from N-glycosylation inhibitor tunicamycin treated ER-/PR-/HER2+ (double negative) breast tumor in athymic nude mice exhibited reduced N-glycan but increased GRP78 expression. We have evaluated the effect of tunicamycin on cellular localization of GRP78 in metastatic human breast cancer cells MDA-MB-231 (ER-/PR-/HER2-)...
December 1, 2017: Glycobiology
https://www.readbyqxmd.com/read/29205553/vitronectin-vn-glycosylation-patterned-by-lectin-affinity-assays-a-potent-glycoproteomic-tool-to-discriminate-plasma-vn-from-cancer-ascites-vn
#16
H Benachour, J Leroy-Dudal, R Agniel, J Wilson, M Briand, F Carreiras, O Gallet
Changes in glycosylation have been associated with human cancer, but their complexity poses an analytical challenge. Ovarian cancer is a major cause of death in women because of an often late diagnosis. At least one-third of patients presents ascites fluid at diagnosis, and almost all have ascites at recurrence. Vitronectin (Vn) is a multifunctional glycoprotein that is suggested to be implicated in ovarian cancer metastasis and is found within ascites. The present study evaluated the potential of using lectin affinity for characterizing the glycosylation pattern of Vn...
December 4, 2017: Journal of Molecular Recognition: JMR
https://www.readbyqxmd.com/read/29203881/in-vivo-tropism-of-salmonella-typhi-toxin-to-cells-expressing-a-multiantennal-glycan-receptor
#17
Yi-An Yang, Sohyoung Lee, Jun Zhao, Andrew J Thompson, Ryan McBride, Buyankhishig Tsogtbaatar, James C Paulson, Ruth Nussinov, Lingquan Deng, Jeongmin Song
Typhoid fever is a life-threatening disease, but little is known about the molecular bases for its unique clinical presentation. Typhoid toxin, a unique virulence factor of Salmonella Typhi (the cause of typhoid fever), recapitulates in an animal model many symptoms of typhoid fever. Typhoid toxin binding to its glycan receptor Neu5Ac is central, but, due to the ubiquity of Neu5Ac, how typhoid toxin causes specific symptoms remains elusive. Here we show that typhoid toxin displays in vivo tropism to cells expressing multiantennal glycoprotein receptors, particularly on endothelial cells of arterioles in the brain and immune cells, which is in line with typhoid symptoms...
December 4, 2017: Nature Microbiology
https://www.readbyqxmd.com/read/29202487/maillard-neoglycans-as-inhibitors-for-in-vitro-adhesion-of-f4-enterotoxigenic-escherichia-coli-to-piglet-intestinal-cells
#18
Héctor Manuel Sarabia-Sainz, Verónica Mata Haro, José Andre-I Sarabia Sainz, Luz Vázquez-Moreno, Gabriela Ramos-Clamont Montfort
Adhesion of enterotoxigenic (ETEC) E. coli to host intestinal cells is mediated by lectin-like fimbriae that bind to specific glycan moieties on the surfaces of enterocytes. To prevent in vitro binding of E. coli F4 fimbriae (F4 ETEC+) to piglet enterocytes, neoglycans were synthesized by the Maillard reaction conjugating lactose (Lac), galacto-oligosaccharides (GOS) or chitin oligosaccharides (Ochit) to porcine serum albumin (PSA). Neoglycans were characterized by SDS-PAGE, intrinsic tryptophan fluorescence and recognition by plant lectins, as well as by F4 ETEC variants...
December 5, 2017: Acta Biochimica Polonica
https://www.readbyqxmd.com/read/29202472/hyposialylated-igg-activates-endothelial-igg-receptor-fc%C3%AE-riib-to-promote-obesity-induced-insulin-resistance
#19
Keiji Tanigaki, Anastasia Sacharidou, Jun Peng, Ken L Chambliss, Ivan S Yuhanna, Debabrata Ghosh, Mohamed Ahmed, Alexander J Szalai, Wanpen Vongpatanasin, Robert F Mattrey, Qiushi Chen, Parastoo Azadi, Ildiko Lingvay, Marina Botto, William L Holland, Jennifer J Kohler, Shashank R Sirsi, Kenneth Hoyt, Philip W Shaul, Chieko Mineo
Type 2 diabetes mellitus (T2DM) is a common complication of obesity. Here, we have shown that activation of the IgG receptor FcγRIIB in endothelium by hyposialylated IgG plays an important role in obesity-induced insulin resistance. Despite becoming obese on a high-fat diet (HFD), mice lacking FcγRIIB globally or selectively in endothelium were protected from insulin resistance as a result of the preservation of insulin delivery to skeletal muscle and resulting maintenance of muscle glucose disposal. IgG transfer in IgG-deficient mice implicated IgG as the pathogenetic ligand for endothelial FcγRIIB in obesity-induced insulin resistance...
November 27, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29201607/in-situ-ligation-of-high-and-low-affinity-ligands-to-cell-surface-receptors-enables-highly-selective-recognition
#20
Misako Taichi, Shogo Nomura, Ikuhiko Nakase, Rie Imamaki, Yasuhiko Kizuka, Fumi Ota, Naoshi Dohmae, Shinobu Kitazume, Naoyuki Taniguchi, Katsunori Tanaka
This paper reports an entirely unexplored concept of simultaneously recognizing two receptors using high- and low-affinity ligands through ligating them in situ on the target cell surface. This de novo approach is inspired by the pretargeting strategy frequently applied in molecular imaging, and has now evolved as the basis of a new paradigm for visualizing target cells with a high imaging contrast. A distinct advantage of using a labeled low-affinity ligand such as glycan is that the excess labeled ligand can be washed away from the cells, whereas the ligand bound to the cell, even at the milli molar affinity level, can be anchored by a bioorthogonal reaction with a pretargeted high-affinity ligand on the surface...
November 2017: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
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