keyword
MENU ▼
Read by QxMD icon Read
search

EMS Shrimp

keyword
https://www.readbyqxmd.com/read/29056496/the-spread-of-vibrio-parahaemolyticus-in-tissues-of-the-pacific-white-shrimp-litopenaeus-vannamei-analyzed-by-pcr-and-histopathology
#1
Umaporn Khimmakthong, Pimwarang Sukkarun
V. parahaemolyticus are bacteria that cause the Acute Hepatopancreatic Necrosis Disease (AHPND), or Early Mortality Syndrome (EMS), in shrimp. To further understand the pathogenesis mechanisms of V. parahaemolyticus infection in shrimp, the spreading of this bacterium in various tissues was investigated. The spread of infection in shrimp that were exposed to seawater bacteria was studied by PCR and histopathology at 1 min, 1, 6, 12, 24, 48 and 72 h after exposure. The PCR results showed that V. parahaemolyticus was at its most widespread at 6 h after exposure, at which point V...
October 19, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28924190/microbiome-of-pacific-whiteleg-shrimp-reveals-differential-bacterial-community-composition-between-wild-aquacultured-and-ahpnd-ems-outbreak-conditions
#2
Fernanda Cornejo-Granados, Alonso A Lopez-Zavala, Luigui Gallardo-Becerra, Alfredo Mendoza-Vargas, Filiberto Sánchez, Rodrigo Vichido, Luis G Brieba, Maria Teresa Viana, Rogerio R Sotelo-Mundo, Adrián Ochoa-Leyva
Crustaceans form the second largest subphylum on Earth, which includes Litopeneaus vannamei (Pacific whiteleg shrimp), one of the most cultured shrimp worldwide. Despite efforts to study the shrimp microbiota, little is known about it from shrimp obtained from the open sea and the role that aquaculture plays in microbiota remodeling. Here, the microbiota from the hepatopancreas and intestine of wild type (wt) and aquacultured whiteleg shrimp and pond sediment from hatcheries were characterized using sequencing of seven hypervariable regions of the 16S rRNA gene...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28625840/survival-of-litopenaeus-vannamei-shrimp-fed-on-diets-supplemented-with-dunaliella-sp-is-improved-after-challenges-by-vibrio-parahaemolyticus
#3
Diana Medina Félix, José Antonio López Elías, Ángel Isidro Campa Córdova, Luis Rafael Martínez Córdova, Antonio Luna González, Edilmar Cortes Jacinto, Nolberta Huerta Aldaz, Fernando Cano Mendoza, María Guadalupe Burboa Zazueta
Survival of Litopenaeus vannamei was evaluated during a Vibrio parahaemolyticus infection. This bacteria has been causing significant economic losses in the shrimp industry due to the appearance of early mortality syndrome (EMS), also known as acute hepatopancreatic necrosis disease (AHPND). Control of bacteria in ponds is difficult to achieve with antibiotics due to environmental infections and antibiotic resistance. New methods have been proposed to control and prevent the impact of bacterial infections. The physiological response indicated by plasma biochemical parameters in shrimp can determine their health and stress status...
September 2017: Journal of Invertebrate Pathology
https://www.readbyqxmd.com/read/28576761/a-natural-vibrio-parahaemolyticus-pir-vp-a-b-mutant-kills-shrimp-but-produces-no-pir-vp-toxins-or-ahpnd-lesions
#4
Kornsunee Phiwsaiya, Walaiporn Charoensapsri, Suwimon Taengphu, Ha T Dong, Pakkakul Sangsuriya, Giang T T Nguyen, Hung Q Pham, Piti Amparyup, Kallaya Sritunyalucksana, Suparat Taengchaiyaphum, Parin Chaivisuthangkura, Siwaporn Longyant, Paisarn Sithigorngul, Saengchan Senapin
Acute hepatopancreatic necrosis disease (AHPND) of shrimp is caused by Vibrio parahaemolyticus (VP) isolates that harbor a pVA plasmid encoding toxins Pir(vp)A and Pir(vp)B (VPAHPND). These are released from VPAHPND that colonize the shrimp stomach and produce pathognomonic AHPND lesions (massive sloughing of hepatopancreatic tubule epithelial cells). PCR results indicated that VP isolate XN87 lacked Pir(vp)A but carried Pir(vp)B Unexpectedly, western blot analysis of proteins from culture broth of XN87 revealed absence of both toxins and the lack of Pir(vp)B was further confirmed by ELISA assay...
June 2, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27594170/impact-of-farm-management-on-expression-of-early-mortality-syndrome-acute-hepatopancreatic-necrosis-disease-ems-ahpnd-on-penaeid-shrimp-farms-in-thailand
#5
V Boonyawiwat, T Patanasatienkul, J Kasornchandra, C Poolkhet, S Yaemkasem, L Hammell, J Davidson
Asian shrimp farming industry has experienced massive production losses due to a disease caused by toxins of Vibrio bacteria, known as early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) for the last 5 years. The disease can cause up to 100% cumulative pond mortality within a week. The objective of this study was to identify factors associated with AHPND occurrence on shrimp farms. A case-control study was carried out on shrimp farms in four provinces of Thailand. Factors related to farm characteristics, farm management, pond and water preparation, feed management, post-larvae (PL) shrimp and stock management were evaluated...
May 2017: Journal of Fish Diseases
https://www.readbyqxmd.com/read/27570736/draft-genome-sequence-of-pathogenic-bacteria-vibrio-parahaemolyticus-strain-ba94c2-associated-with-acute-hepatopancreatic-necrosis-disease-isolate-from-south-america
#6
Leda Restrepo, Bonny Bayot, Irma Betancourt, Andres Pinzón
Vibrio parahaemolyticus is a pathogenic bacteria which has been associated to the early mortality syndrome (EMS) also known as hepatopancreatic necrosis disease (AHPND) causing high mortality in shrimp farms. Pathogenic strains contain two homologous genes related to insecticidal toxin genes, PirA and PirB, these toxin genes are located on a plasmid contained within the bacteria. Genomic sequences have allowed the finding of two strains with a divergent structure related to the geographic region from where they were found...
September 2016: Genomics Data
https://www.readbyqxmd.com/read/27221155/a-novel-method-of-real-time-reverse-transcription-loop-mediated-isothermal-amplification-developed-for-rapid-and-quantitative-detection-of-a-new-genotype-yhv-8-of-yellow-head-virus
#7
H-L Yang, L Qiu, Q Liu, X-Y Wan, S Liu, L-L Zhu, B Yang, Q-L Zhang, J Huang
UNLABELLED: A new genotype of yellow head virus (YHV), designated as YHV-8, was found in farmed shrimp Fenneropenaeus chinensis suffering suspectedly from EMS/AHPNS (early mortality disease/acute hepatopancreatic necrosis disease) in China in 2012. In this study, a one-step, real-time reverse-transcription loop-mediated isothermal amplification (rRT-LAMP) assay was developed for better detection of both genotypes of YHV-1 and YHV-8. A set of six specific primers was successfully designed targeting a conserved region of the YHV genome...
August 2016: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/27003504/sensitive-visual-detection-of-ahpnd-bacteria-using-loop-mediated-isothermal-amplification-combined-with-dna-functionalized-gold-nanoparticles-as-probes
#8
Narong Arunrut, Jantana Kampeera, Sarawut Sirithammajak, Piyachat Sanguanrut, Porranee Proespraiwong, Rungkarn Suebsing, Wansika Kiatpathomchai
Acute hepatopancreatic necrosis disease (AHPND) is a component cause of early mortality syndrome (EMS) of shrimp. In 2013, the causative agent was found to be unique isolates of Vibrio parahaemolyticus (VPAHPND) that contained a 69 kbp plasmid (pAP1) carrying binary Pir-like toxin genes PirvpA and PirvpB. In Thailand, AHPND was first recognized in 2012, prior to knowledge of the causative agent, and it subsequently led to a precipitous drop in shrimp production. After VPAHPND was characterized, a major focus of the AHPND control strategy was to monitor broodstock shrimp and post larvae for freedom from VPAHPND by nucleic acid amplification methods, most of which required use of expensive and sophisticated equipment not readily available in a shrimp farm setting...
2016: PloS One
https://www.readbyqxmd.com/read/26555534/prevalence-and-antimicrobial-resistance-of-vibrio-spp-in-retail-and-farm-shrimps-in-ecuador
#9
L Sperling, T Alter, S Huehn
The aim of this study was to investigate the prevalence of Vibrio spp. in shrimp at retail and in shrimp farms in Ecuador and to determine the antimicrobial agent resistance patterns of farm isolates. The presence of genes linked to early mortality syndrome (EMS) or acute hepatopancreatic necrosis disease (AHPND) also was evaluated. Vibrio spp. were isolated from retail shrimps in Cuenca, Ecuador, and farm shrimps originating from provinces El Oro and Guayas, Ecuador. A total of 229 shrimp samples were collected, of which 71 originated from retail markets in Cuenca and 158 came from shrimp farms...
November 2015: Journal of Food Protection
https://www.readbyqxmd.com/read/26549178/pathogenesis-of-acute-hepatopancreatic-necrosis-disease-ahpnd-in-shrimp
#10
Hung-Chiao Lai, Tze Hann Ng, Masahiro Ando, Chung-Te Lee, I-Tung Chen, Jie-Cheng Chuang, Rapeepat Mavichak, Sheng-Hsiung Chang, Mi-De Yeh, Yi-An Chiang, Haruko Takeyama, Hiro-o Hamaguchi, Chu-Fang Lo, Takashi Aoki, Han-Ching Wang
Acute hepatopancreatic necrosis disease (AHPND), also called early mortality syndrome (EMS), is a recently emergent shrimp bacterial disease that has resulted in substantial economic losses since 2009. AHPND is known to be caused by strains of Vibrio parahaemolyticus that contain a unique virulence plasmid, but the pathology of the disease is still unclear. In this study, we show that AHPND-causing strains of V. parahaemolyticus secrete the plasmid-encoded binary toxin PirAB(vp) into the culture medium. We further determined that, after shrimp were challenged with AHPND-causing bacteria, the bacteria initially colonized the stomach, where they started to produce PirAB(vp) toxin...
December 2015: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/25667334/photorhabdus-insect-related-pir-toxin-like-genes-in-a-plasmid-of-vibrio-parahaemolyticus-the-causative-agent-of-acute-hepatopancreatic-necrosis-disease-ahpnd-of-shrimp
#11
Jee Eun Han, Kathy F J Tang, Loc H Tran, Donald V Lightner
The 69 kb plasmid pVPA3-1 was identified in Vibrio parahaemolyticus strain 13‑028/A3 that can cause acute hepatopancreatic necrosis disease (AHPND). This disease is responsible for mass mortalities in farmed penaeid shrimp and is referred to as early mortality syndrome (EMS). The plasmid has a GC content of 45.9% with a copy number of 37 per bacterial cell as determined by comparative quantitative PCR analyses. It consists of 92 open reading frames that encode mobilization proteins, replication enzymes, transposases, virulence-associated proteins, and proteins similar to Photorhabdus insect-related (Pir) toxins...
February 10, 2015: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/25517999/endogenous-molecules-induced-by-a-pathogen-associated-molecular-pattern-pamp-elicit-innate-immunity-in-shrimp
#12
Yu-Yuan Chen, Jiann-Chu Chen, Yong-Chin Lin, Suwaree Kitikiew, Hui-Fang Li, Jia-Chin Bai, Kuei-Chi Tseng, Bo-Wei Lin, Po-Chun Liu, Yin-Ze Shi, Yi-Hsuan Kuo, Yu-Hsuan Chang
Invertebrates rely on an innate immune system to combat invading pathogens. The system is initiated in the presence of cell wall components from microbes like lipopolysaccharide (LPS), β-1,3-glucan (βG) and peptidoglycan (PG), altogether known as pathogen-associated molecular patterns (PAMPs), via a recognition of pattern recognition protein (PRP) or receptor (PRR) through complicated reactions. We show herein that shrimp hemocytes incubated with LPS, βG, and PG caused necrosis and released endogenous molecules (EMs), namely EM-L, EM-β, and EM-P, and found that shrimp hemocytes incubated with EM-L, EM-β, and EM-P caused changes in cell viability, degranulation and necrosis of hemocytes, and increased phenoloxidase (PO) activity and respiratory burst (RB) indicating activation of immunity in vitro...
2014: PloS One
https://www.readbyqxmd.com/read/25144120/detection-of-acute-hepatopancreatic-necrosis-disease-ahpnd-in-mexico
#13
Linda Nunan, Donald Lightner, Carlos Pantoja, Silvia Gomez-Jimenez
Acute hepatopancreatic necrosis disease (AHPND), which has also been referred to as early mortality syndrome (EMS), initially emerged as a destructive disease of cultured shrimp species in Asia in 2009. The pathogen associated with the disease, Vibrio parahaemolyticus, subsequently spread to the Western Hemisphere and emerged in Mexico in early 2013. The spread to the Western Hemisphere is a major concern to shrimp producers in the region. To date, the only peer-reviewed published method for determining whether mortalities are due to AHPND is through histological examination...
August 21, 2014: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/24604636/draft-genome-sequence-of-vibrio-parahaemolyticus-strain-m0605-which-causes-severe-mortalities-of-shrimps-in-mexico
#14
Bruno Gomez-Gil, Sonia Soto-Rodríguez, Rodolfo Lozano, Miguel Betancourt-Lozano
Acute hepatopancreatic necrosis disease (AHPND), also known as early mortality syndrome (EMS), causes high mortalities in cultured shrimps in Asia (L. Tran et al., Dis. Aquat. Organ. 105:45-55, 2013, http://dx.doi.org/10.3354/dao02621). Here, we report the draft genome sequence of one Mexican strain of Vibrio parahaemolyticus that causes similar clinical signs in diseased shrimps.
2014: Genome Announcements
https://www.readbyqxmd.com/read/23872504/effects-of-genotoxicity-and-its-consequences-at-the-population-level-in-sexual-and-asexual-artemia-assessed-by-analysis-of-inter-simple-sequence-repeats-issr
#15
Sandhya Sukumaran, Alastair Grant
There is considerable evidence that genetic damage in organisms occurs in the environment as a result of exposure to genotoxins and ionising radiation, but we have limited understanding of the extent to which this results in adverse consequences at a population level. We used inter-simple sequence repeat (ISSR) markers to quantify genotoxic effects of the mutagen ethylmethane sulfonate (EMS) on a sexual (Artemia franciscana) and an asexual (Artemia parthenogenetica) species of brine shrimp. The method provides information similar to that obtained with assessment of RAPD (random amplification of polymorphic DNA) but is more robust...
September 18, 2013: Mutation Research
https://www.readbyqxmd.com/read/23836769/determination-of-the-infectious-nature-of-the-agent-of-acute-hepatopancreatic-necrosis-syndrome-affecting-penaeid-shrimp
#16
Loc Tran, Linda Nunan, Rita M Redman, Leone L Mohney, Carlos R Pantoja, Kevin Fitzsimmons, Donald V Lightner
A new emerging disease in shrimp, first reported in 2009, was initially named early mortality syndrome (EMS). In 2011, a more descriptive name for the acute phase of the disease was proposed as acute hepatopancreatic necrosis syndrome (AHPNS). Affecting both Pacific white shrimp Penaeus vannamei and black tiger shrimp P. monodon, the disease has caused significant losses in Southeast Asian shrimp farms. AHPNS was first classified as idiopathic because no specific causative agent had been identified. However, in early 2013, the Aquaculture Pathology Laboratory at the University of Arizona was able to isolate the causative agent of AHPNS in pure culture...
July 9, 2013: Diseases of Aquatic Organisms
1
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"