Oliveira jr

Acanthoscurria juruenicola is an Amazonian spider described for the first time almost a century ago. However, little is known about their venom composition. Here, we present a multiomics characterization of A. juruenicola venom by a combination of transcriptomics, proteomics, and peptidomics approaches. Transcriptomics of female venom glands resulted in 93,979 unique assembled mRNA transcript encoding proteins. A total of 92 proteins were identified in the venom by mass spectrometry, including 14 mature cysteine-rich peptides (CRPs)...

Nutritional restriction during developmental periods impairs organ physiology. Female rats were subjected to protein restriction during pregnancy and lactation to analyze dental and maxillary development. Four exposure groups were considered: normal-protein diet during pregnancy and lactation (NP, 17% casein), low-protein diet during lactation (LP-L, 6% casein), low-protein diet during pregnancy and lactation (LP), and low-protein diet during pregnancy (LP-G). Maxillae from 15-day-old male pups were collected...

OBJECTIVE: To evaluate the influence of anteroposterior position of the soft tissue on facial attractiveness in black people, and compare the perception of aesthetics and satisfaction between orthodontists and black laypersons. METHODS: The sample was composed of 69 orthodontists and 69 laypersons of black ethnicity (n=138). Facial profile photographs of two black volunteers, a man and a woman, were digitally manipulated to change the position of the lips and chin, by making gradual changes of 4mm in relation to the true vertical line, simulating advance or retrusion of the soft tissues by -2, -6, -10, +2, +6, +10mm, totalling six images per sex...

The Internet of Things (IoT) is based on objects or "things" that have the ability to communicate and transfer data. Due to the large number of connected objects and devices, there has been a rapid growth in the amount of data that are transferred over the Internet. To support this increase, the heterogeneity of devices and their geographical distributions, there is a need for IoT gateways that can cope with this demand. The SOFTWAY4IoT project, which was funded by the National Education and Research Network (RNP), has developed a software-defined and virtualized IoT gateway that supports multiple wireless communication technologies and fog/cloud environment integration...

Advances in Artificial Reproductive Technologies (ARTs) in bovine embryos to produce cloned pregnancies have been developed in the last years, however high pregnancy losses rates still present. Those rates are associated to placental morphology alterations that are majorly focused on extracellular matrix (ECM) alterations and consequently placentome hyperplasia, increased trophoblast cell migration and vascular defects. Herein, we aimed to search, at protein level, pathways altered by ART that can modify the placental development harmony...

Introduction: Sickle cell disease is the most prevalent hereditary disease in the country. The aim of this study was to use transcranial Doppler as a screening method for identifying cerebral vasculopathy in children with sickle cell disease. Methods: An epidemiologic, descriptive, and cross-sectional study was conducted. Patients aged 2-16 years with sickle cell disease and followed at a neurology referral service between January 2014 and March 2020 underwent transcranial Doppler and complementary examinations to screen for cerebral vasculopathy...

The Iguaçu River basin presents high ecological importance due to its expressive endemic ichthyofauna rate, but chemical pollution may threat this biodiversity. Jordão River is one of the main tributaries of Iguaçu River and contribute to this pollution status, since it drains large agricultural areas receiving domestic and industrial effluents before flowing into the Iguaçu River. The objective of the current study was to evaluate the toxic effects of the Iguaçu, Jordão, and the combination of their waters to the embryo-larval phase of R...

Endometriosis is a chronic inflammatory disorder that is highly associated with infertility. This association seems to be related to oocyte impairment, mainly in the initial stages of endometriosis (minimal and mild), where no distortions or adhesions are present. Nonetheless, invasive oocyte analyses are not routinely feasible; thus, indirect assessment of oocyte quality is highly desirable, and, in this context, cumulus cells (CCs) may be more suitable targets of analysis. CCs are crucial in oocyte development and could be used as an index of oocyte quality...

Achieving nutritional adequacy requires an increase in fresh foods consumption, which may increase pesticide intakes. This study aimed to identify required dietary modifications to achieve nutritional adequacy without exceeding the acceptable daily intake (ADI) for pesticides. Data from the National Dietary Survey 2017-2018 were linked to the pesticide database from the Program on Pesticide Residue Analysis in Food. We performed linear programming models to design nutritionally adequate diets constrained by food preferences for different constraints on pesticide intake at the least cost increment...

Among the Chilopoda class of centipede, the Cryptops genus is one of the most associated with envenomation in humans in the metropolitan region of the state of São Paulo. To date, there is no study in the literature about the toxins present in its venom. Thus, in this work, a transcriptomic characterization of the Cryptops iheringi venom gland, as well as a proteomic analysis of its venom, were performed to obtain a toxin profile of this species. These methods indicated that 57.9% of the sequences showed to be putative toxins unknown in public databases; among them, we pointed out a novel putative toxin named Cryptoxin-1...

This article presents an approach to autonomous flight planning of Unmanned Aerial Vehicles (UAVs)-Drones as data collectors to the Internet of Things (IoT). We have proposed a model for only one aircraft, as well as for multiple ones. A clustering technique that extends the scope of the number of IoT devices (e.g., sensors) visited by UAVs is also addressed. The flight plan generated from the model focuses on preventing breakdowns due to a lack of battery charge to maximize the number of nodes visited. In addition to the drone autonomous flight planning, a data storage limitation aspect is also considered...

Congenital myopathies are a heterogeneous group of conditions diagnosed based on the clinical presentation, muscle histopathology and genetic defects. Recessive mutations in the SPEG gene have been described in recent years and are primarily associated with centronuclear myopathy with cardiomyopathy. In this report, we describe two Brazilian siblings, aged 13 and 6 years, with a novel homozygous mutation (c.8872 C>T:p.Arg2958Ter) in the SPEG gene leading to a congenital myopathy. In the older sibling, the muscle biopsy showed fiber size disproportion...

Introduction: This study aimed to investigate the effect of low-level laser therapy (LLLT) on the blood cell count when applied to parotid glands of rats irradiated by volumetric modular arc therapy (VMAT). Methods: Thirty-two adult male Wistar rats were used in this study. Samples were randomly assigned to three groups: control group (CG, n = 8), immediate laser group (24 hours) (ILG, n=12), and late laser group (120 hours) (LLG, n=12). The two laser groups were previously subjected to VMAT radiotherapy in a single dose of 12 Gy...

To report a rare case of a patient diagnosed with segmental arterial mediolysis with abdominal angina. The work is characterized as a Case Report with bibliographic review, the method used was the bibliometric analysis of articles published in the Pubmed database. The theoretical scan covered the years 2010 to 2020, and consisted of seeking out articles throughout all indexes using the terms: segmental arterial mediolysis and abdominal angina. The search occurred between the months of October and December of 2020...

OBJECTIVE: To evaluate the anesthetic effects of two drug combinations with local anesthesia, with or without postoperative antagonists, for orchiectomy in cats. STUDY DESIGN: Prospective, randomized blinded clinical study. ANIMALS: A total of 64 healthy cats. METHODS: Cats were assigned to four equal groups: ketamine (5 mg kg-1 ) and dexmedetomidine (10 μg kg-1 ) were administered intramuscularly (IM), followed postoperatively with intravenous (IV) saline (5 mL; group KDS) or atipamezole (50 μg kg-1 ; group KDA); and ketamine (14 mg kg-1 ) with midazolam (0...

The aim of this pilot study was to evaluate the impact of the association of free gingival graft (FGG) or collagen-matrix xenograft (CMX) to deproteinized bovine bone graft (DBBG) on the preservation of post-extraction sockets with facial-wall defects. Sixteen patients who presented a maxillary tooth with a facial bone defect and indication of extraction were selected. After the surgical procedure, all the post-extraction sockets were filled with DBBG and covered with a collagen membrane. The cervical part of the socket was then sealed with either FGG or CMX...

The aim of this pilot study was to evaluate the impact of the association of free gingival graft (FGG) or collagen-matrix xenograft (CMX) to deproteinized bovine bone graft (DBBG) on the preservation of post-extraction sockets with facial-wall defects. Sixteen patients who presented a maxillary tooth with a facial bone defect and indication of extraction were selected. After the surgical procedure, all the post-extraction sockets were filled with DBBG and covered with a collagen membrane. The cervical part of the socket was then sealed with either FGG or CMX...

INTRODUCTION: Antimicrobial stewardship programs are necessary practices to combat resistance in hospital infections. However, studies frequently cite the scarcity of technological resources as an obstacle to the comprehensive development of ASPs. OBJECTIVES: The aim of the study was to assess the impact of monitoring software on reducing antibiotic consumption and reducing resistance in a pediatric intensive care unit. METHODS: We conducted an analytical longitudinal study during the period January 1, 2007, to December 31, 2018, in the pediatric intensive care unit of a high-complexity tertiary general hospital in Brazil...

Most research in plant chronobiology has been done in laboratory conditions. However, they usually fail to mimic natural conditions and their slight fluctuations, highlighting or obfuscating rhythmicity. High-density crops, such as sugarcane (Saccharum hybrid), generate field microenvironments with specific light and temperature due to mutual shading. We measured the metabolic and transcriptional rhythms in the leaves of 4-month-old (4 mo) and 9 mo sugarcane grown in the field. Most of the assayed rhythms in 9 mo sugarcane peaked >1 h later than in 4 mo sugarcane, including rhythms of the circadian clock gene, LATE ELONGATED HYPOCOTYL (LHY)...

Industry 4.0, allied with the growth and democratization of Artificial Intelligence (AI) and the advent of IoT, is paving the way for the complete digitization and automation of industrial processes. Maintenance is one of these processes, where the introduction of a predictive approach, as opposed to the traditional techniques, is expected to considerably improve the industry maintenance strategies with gains such as reduced downtime, improved equipment effectiveness, lower maintenance costs, increased return on assets, risk mitigation, and, ultimately, profitable growth...