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Paula Frid, Knut Tornes, Øyunn Nielsen, Nils Skaug
OBJECTIVES: The aims of this study were to investigate the infectious etiology of primary chronic osteomyelitis (PCO) of the jaw using an extraoral sampling approach and molecular methods. STUDY DESIGN: In this pilot study, bone biopsies from the mandibles of 5 patients with PCO were sampled with an extraoral sterile approach. Cultivation and polymerase chain reaction (PCR) were performed. RESULTS: Two of the biopsies yielded growth of Propionebacterium acnes...
May 2009: Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics
P Sørensen, T Ejlertsen, D Aaen, K Poulsen
Bacterial infection is a major cause of shunt dysfunction. It is well-known that the majority of pathogenic micro-organisms are low-virulent bacteria normally found on intact skin. Probably shunts become contaminated during surgery either by contact to the patient skin, or contact from contaminated gloves or instruments. This study was performed to find out to what extent gloves become contaminated during shunt surgery. Gloves used during shunt implantation were examined in 10 operations. Shunt implantation was done using recommended precautions to avoid infection, including prophylactic antibiotics and double gloving, by surgeons experienced in shunt surgery...
October 2008: British Journal of Neurosurgery
Hector E James, John S Bradley
OBJECTIVE: This report is limited to patients with a single cerebrospinal fluid (CSF) shunt infected by a single organism, and compares two treatment protocols. METHODS: In the initial protocol (1975-1991), patients underwent removal of the shunt system and received intravenous and intraventricular antibiotics. Intraventricular antibiotics were administered twice daily to those with external ventricular drainage. When CSF was cultured 48 h off all antibiotics and found to be sterile at 24 h of incubation, a new shunt was inserted...
2008: Pediatric Neurosurgery
A S MacDonald, A D Straw, B Bauman, E J Pearce
Whether dendritic cells (DC) play a passive or active role in Th2 response induction is poorly understood. In this study, we show that CD8- DC pulsed with Th2-polarizing Ag (soluble egg Ag (SEA)) from Schistosoma mansoni potently stimulate Th2 responses in vivo and in vitro while failing to undergo a conventional maturation process. Thus, in contrast to DC pulsed with the Th1 response inducing Ag Propionebacterium acnes, SEA-exposed DC exhibit a phenotype that is most similar to that of immature DC, failing to up-regulate expression of CD40, CD54, CD80, CD86, or OX40L; producing no detectable IL-4, IL-10, or IL-12; and displaying only a minor increase in MHC class II expression...
August 15, 2001: Journal of Immunology: Official Journal of the American Association of Immunologists
H Westergren, V Westergren, U Forsum
We have examined the accuracy of our present CSF sampling technique in 14 referred patients with suspect shunt dysfunction. The study was performed comparing culture results from shunt reservoir punctures and sham samples from skin tissue fluid close to the reservoirs. Propionebacterium acnes (P acnes) was found in five of fifteen cultures from reservoir punctures and in five of fifteen sham procedures. Positive cultures correlated in two cases. Thirteen of the fourteen patients included in the study were followed clinically and their symptoms disappeared without antibiotic or surgical treatment...
1997: Acta Neurochirurgica
J G Lee, K S Kim
It has been known for many years that tooth extraction can cause bacterial dissemination into blood stream, that is, bacteremia. Besides endocarditis--causing bacteria, alpha-hemolytic streptococci, anaerobes are involved in the post- extraction bacteremia. They are of clinical importance because they can cause abscesses in the various parenchymatous organs such as the liver, lung, and brain through anachoresis. The purpose of this study is to establish the types of species of anaerobes associated with post--extraction bacteremia, and to give the baseline to the succeeding study of sensitivity tests of various antibiotics, thus clinicians can chose the right ones...
March 1990: Taehan Ch'ikkwa Uisa Hyŏphoe Chi
M L Nielsen, D Raahave, J G Stage, T Justesen
The amount, composition, and localization of anaerobic and aerobic bacteria in the normal skin before and after disinfection were the subject of a volunteer study. The superficial bacterial flora were sampled by velvet pad imprints, and the deep flora were determined from whole skin biopsies. Only one anaerobic species, Propionebacterium acnes, was encountered even though other and more strict anaerobic bacteria could have been grown with the anaerobic technique employed. Staphylococcus albus dominated among the aerobic superficial bacteria, while diphtheroids, Micrococcus spp...
October 1975: Journal of Clinical Pathology
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