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https://www.readbyqxmd.com/read/28919552/accurate-identification-of-anopheles-gambiae-giles-trophic-preferences-by-maldi-tof-ms
#1
Sirama Niare, Fatalmoudou Tandina, Bernard Davoust, Ogobara Doumbo, Didier Raoult, Philippe Parola, Lionel Almeras
The determination of the trophic preferences of the Anopheles gambiae Giles (Diptera: Culicidae) is a decisive parameter for the monitoring and the prevention of malaria risk transmission. Currently, arthropod blood feeding sources are identified using immunological or molecular biology traditional techniques. Despite the effectiveness of these methods, they present several limitations, and notably, they are time-consuming and costly techniques. A recent study demonstrated that MALDI-TOF MS could be a useful tool for the identification of blood meal origins in freshly engorged mosquitoes...
September 15, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28390830/bmajpla2-ii-a-basic-lys49-phospholipase-a2-homologue-from-bothrops-marajoensis-snake-venom-with-parasiticidal-potential
#2
Amy N Grabner, Jorge Alfonso, Anderson M Kayano, Leandro S Moreira-Dill, Ana Paula de A Dos Santos, Cleópatra A S Caldeira, Juliana C Sobrinho, Ana Gómez, Fernando P Grabner, Fabio F Cardoso, Juliana Pavan Zuliani, Marcos R M Fontes, Daniel C Pimenta, Celeste Vega Gómez, Carolina B G Teles, Andreimar M Soares, Leonardo A Calderon
Snake venoms contain various proteins, especially phospholipases A2 (PLA2s), which present potential applications in diverse areas of health and medicine. In this study, a new basic PLA2 from Bothrops marajoensis with parasiticidal activity was purified and characterized biochemically and biologically. B. marajoensis venom was fractionated through cation exchange followed by reverse phase chromatographies. The isolated toxin, BmajPLA2-II, was structurally characterized with MALDI-TOF (Matrix-assisted laser desorption/ionization-time of flight) mass spectrometry, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), followed by two-dimensional electrophoresis, partial amino acid sequencing, an enzymatic activity assay, circular dichroism, and dynamic light scattering assays...
September 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28049524/maldi-tof-ms-as-an-innovative-tool-for-detection-of-plasmodium-parasites-in-anopheles-mosquitoes
#3
Maureen Laroche, Lionel Almeras, Emilie Pecchi, Yassina Bechah, Didier Raoult, Angèle Viola, Philippe Parola
BACKGROUND: Malaria is still a major public health issue worldwide, and one of the best approaches to fight the disease remains vector control. The current methods for mosquito identification include morphological methods that are generally time-consuming and require expertise, and molecular methods that require laboratory facilities with relatively expensive running costs. Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) technology, routinely used for bacterial identification, has recently emerged in the field of entomology...
January 3, 2017: Malaria Journal
https://www.readbyqxmd.com/read/27602567/towards-a-proteomic-catalogue-and-differential-annotation-of-salivary-gland-proteins-in-blood-fed-malaria-vector-anopheles-culicifacies-by-mass-spectrometry
#4
Ritu Rawal, Sonam Vijay, Kavita Kadian, Jagbir Singh, Veena Pande, Arun Sharma
In order to understand the importance of functional proteins in mosquito behavior, following blood meal, a baseline proteomic dataset is essential for providing insights into the physiology of blood feeding. Therefore, in this study as first step, in solution and 1-D electrophoresis digestion approach combined with tandem mass spectrometry (nano LC-MS/MS) and computational bioinformatics for data mining was used to prepare a baseline proteomic catalogue of salivary gland proteins of sugar fed An. culicifacies mosquitoes...
2016: PloS One
https://www.readbyqxmd.com/read/27072633/differential-protein-expression-in-the-midgut-of-culex-quinquefasciatus-mosquitoes-induced-by-the-insecticide-temephos
#5
P D Games, S N Alves, B B Katz, J M Tomich, J E Serrão
Mosquitoes are vectors for pathogens of malaria, lymphatic filariasis, dengue, chikungunya, yellow fever and Japanese encephalitis. Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) is a known vector of lymphatic filariasis. Its control in Brazil has been managed using the organophosphate temephos. Studies examining the proteins of Cx. quinquefasciatus that are differentially expressed in response to temephos further understanding of the modes of action of the insecticide and may potentially identify resistance factors in the mosquito...
September 2016: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/26872451/identification-of-blood-meal-sources-in-the-main-african-malaria-mosquito-vector-by-maldi-tof-ms
#6
Sirama Niare, Jean-Michel Berenger, Constentin Dieme, Ogobara Doumbo, Didier Raoult, Philippe Parola, Lionel Almeras
BACKGROUND: The identification of blood meal sources in malaria vectors is critical to better understanding host/vector interactions and malaria epidemiology and control. Currently, the identification of mosquito blood meal origins is based on time-consuming and costly techniques such as precipitin tests, ELISA and molecular tools. Although these tools have been validated to identify the blood meal and trophic preferences of female Anopheles mosquitoes, they present several limitations...
February 13, 2016: Malaria Journal
https://www.readbyqxmd.com/read/26721222/ethnobotanical-survey-of-rinorea-dentata-violaceae-used-in-south-western-nigerian-ethnomedicine-and-detection-of-cyclotides
#7
Alfred F Attah, Roland Hellinger, Mubo A Sonibare, Jones O Moody, Sarah Arrowsmith, Susan Wray, Christian W Gruber
ETHNOPHARMACOLOGICAL RELEVANCE: People living in the tropical rain forest of South-Western Nigeria use Rinorea dentata (P. Beauv.) Kuntze (Violaceae) in ethno-veterinary medicine to facilitate parturition. There are no evidence-based pharmacological investigations for the uterotonic activity of this plant. AIMS OF STUDY: (i) Collection of data about the ethnopharmacological uses of R. dentata and evaluation of its uses and applications in health care; (ii) determining potential uterotonic effects in vitro, and (iii) chemical characterization of R...
February 17, 2016: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/26617287/the-fucomic-potential-of-mosquitoes-fucosylated-n-glycan-epitopes-and-their-cognate-fucosyltransferases
#8
Simone Kurz, Jonas G King, Rhoel R Dinglasan, Katharina Paschinger, Iain B H Wilson
Fucoconjugates are key mediators of protein-glycan interactions in prokaryotes and eukaryotes. As examples, N-glycans modified with the non-mammalian core α1,3-linked fucose have been detected in various organisms ranging from plants to insects and are immunogenic in mammals. The rabbit polyclonal antibody raised against plant horseradish peroxidase (anti-HRP) is able to recognize the α1,3-linked fucose epitope and is also known to specifically stain neural tissues in the fruit fly Drosophila melanogaster...
January 2016: Insect Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/26491885/phospholipid-topography-of-whole-body-sections-of-the-anopheles-stephensi-mosquito-characterized-by-high-resolution-atmospheric-pressure-scanning-microprobe-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging
#9
Saleh M Khalil, Andreas Römpp, Jette Pretzel, Katja Becker, Bernhard Spengler
High-resolution atmospheric-pressure scanning microprobe matrix-assisted laser desorption/ionization mass spectrometry imaging (AP-SMALDI MSI) has been employed to study the molecular anatomical structure of rodent malaria vector Anopheles stephensi mosquitoes. A dedicated sample preparation method was developed which suits both, the special tissue properties of the sample and the requirements of high-resolution MALDI imaging. Embedding in 5% carboxymethylcellulose (CMC) was used to maintain the tissue integrity of the whole mosquitoes, being very soft, fragile, and difficult to handle...
November 17, 2015: Analytical Chemistry
https://www.readbyqxmd.com/read/26047717/targeted-release-and-fractionation-reveal-glucuronylated-and-sulphated-n-and-o-glycans-in-larvae-of-dipteran-insects
#10
Simone Kurz, Kazuhiro Aoki, Chunsheng Jin, Niclas G Karlsson, Michael Tiemeyer, Iain B H Wilson, Katharina Paschinger
Mosquitoes are important vectors of parasitic and viral diseases with Anopheles gambiae transmitting malaria and Aedes aegypti spreading yellow and Dengue fevers. Using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction HPLC fractionation followed by MALDI-TOF MS or permethylation followed by NSI-MS), we examined the N-glycans of both A. gambiae and A. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and tri-antennary glycans, some of which are modified with fucose or with sulphate or glucuronic acid residues; the latter anionic modifications were also found on N-glycans of larvae from another dipteran species (Drosophila melanogaster)...
August 3, 2015: Journal of Proteomics
https://www.readbyqxmd.com/read/25975495/a-brief-review-on-biomarkers-and-proteomic-approach-for-malaria-research
#11
Vivek Bhakta Mathema, Kesara Na-Bangchang
Malaria remains as one of the significant health threat to people living in countries throughout tropical and subtropical zones. Proteomic studies of Plasmodium, the protozoan causing malaria, is essential for understanding its cellular structure, growth stage-specific expression of protein metabolites and complex interaction with host. In-depth knowledge of the pathogen is required for identification of novel biomarkers that can be utilized to develop diagnostic tests and therapeutic antimalarial drugs. The alarming rise in drug-resistant strains of Plasmodium has created an urgent need to identify new targets for drug development that can act by obstructing life cycle of this parasite...
April 2015: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/25912083/di-rhamnolipid-is-a-mosquito-pupicidal-metabolite-from-pseudomonas-fluorescens-vcrc-b426
#12
G Prabakaran, S L Hoti, H Surya Prakash Rao, Satish Vijjapu
Pseudomonas fluorescens Migula (VCRC B426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely Culex quinquefasciatus, Anopheles stephensi and Aedes aegypti. The mosquito pupicidal metabolite from P. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, FPLC, HPLC and TLC. The purified metabolite was characterized by NMR, FT-IR, LC-MS and MALDI-TOF. The FT-IR, (1)H and (13)C NMR results showed that it is a rhamnolipid (di-rhamnolipid)...
August 2015: Acta Tropica
https://www.readbyqxmd.com/read/25742511/annotated-differentially-expressed-salivary-proteins-of-susceptible-and-insecticide-resistant-mosquitoes-of-anopheles-stephensi
#13
COMPARATIVE STUDY
Sonam Vijay, Ritu Rawal, Kavita Kadian, Kamaraju Raghavendra, Arun Sharma
Vector control is one of the major global strategies for control of malaria. However, the major obstacle for vector control is the development of multiple resistances to organochlorine, organophosphorus insecticides and pyrethroids that are currently being used in public health for spraying and in bednets. Salivary glands of vectors are the first target organ for human-vector contact during biting and parasite-vector contact prior to parasite development in the mosquito midguts. The salivary glands secrete anti-haemostatic, anti-inflammatory biologically active molecules to facilitate blood feeding from the host and also inadvertently inject malaria parasites into the vertebrate host...
2015: PloS One
https://www.readbyqxmd.com/read/24967929/partial-characterization-of-plasmodium-falciparum-trophozoite-proteome-under-treatment-with-quinine-mefloquine-and-the-natural-antiplasmodial-diosgenone
#14
César Segura, Yesid Cuesta-Astroz, Camila Nunes-Batista, Mariano Zalis, Wanda Maria de Almeida von Krüger, Paulo Mascarello Bisch
INTRODUCTION: Despite efforts to control malaria, around 10% of the world population is at risk of acquiring this disease. Plasmodium falciparum accounts for the majority of severe cases and deaths. Malaria control programs have failed due to the therapeutic failure of first-line antimalarials and to parasite resistance. Thus, new and better therapeutic alternatives are required. Proteomic analysis allows determination of protein expression levels under drug pressure, leading to the identification of new therapeutic drug targets and their mechanisms of action...
April 2014: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/24269164/copper-ion-as-a-delivery-platform-for-taxanes-and-taxane-complexes
#15
Thomas J Manning, Dennis Phillips, Greg Wylie, Benjamin Bythell, Shannon Clark, Ryenne Ogburn, Kaitlyn Ledwitch, Chad Collis, Stephanie Patterson, Landon Lasseter
A number of delivery agents, such as proteins, liposomes, micelles, and nanoparticles, are utilized for transporting pharmaceutical agents in a physiological environment. This Letter focuses on the use of the copper(II) ion and its potential role as a delivery agent for the taxanes and taxol couple to a malaria drug. Nuclear magnetic resonance (NMR, (1)H, (13)C, (15)N), Mass Spectrometry (LC-MS, MALDI-TOF, FT-ICR) and computational methods are used to examine the structure of the complex. The National Cancer Institute's benchmark 60 cell line panel is used to compare the efficacy of the copper-taxol and copper-taxol-hydroxychloroquin complexes to that of iron-taxol and pure taxol...
January 1, 2014: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/24047207/organization-of-plasmodium-falciparum-spliceosomal-core-complex-and-role-of-arginine-methylation-in-its-assembly
#16
Manzar Hossain, Shweta Sharma, Reshma Korde, Shivani Kanodia, Monika Chugh, Khushboo Rawat, Pawan Malhotra
BACKGROUND: Splicing and alternate splicing are the two key biological processes that result in the generation of diverse transcript and protein isoforms in Plasmodium falciparum as well as in other eukaryotic organisms. Not much is known about the organization of splicing machinery and mechanisms in human malaria parasite. Present study reports the organization and assembly of Plasmodium spliceosome Sm core complex. METHODS: Presence of all the seven Plasmodium Sm-like proteins in the intra-erythrocytic stages was assessed based on the protein(s) expression analysis using immuno-localization and western blotting...
2013: Malaria Journal
https://www.readbyqxmd.com/read/23469000/identification-of-cryptic-anopheles-mosquito-species-by-molecular-protein-profiling
#17
Pie Müller, Valentin Pflüger, Matthias Wittwer, Dominik Ziegler, Fabrice Chandre, Frédéric Simard, Christian Lengeler
Vector control is the mainstay of malaria control programmes. Successful vector control profoundly relies on accurate information on the target mosquito populations in order to choose the most appropriate intervention for a given mosquito species and to monitor its impact. An impediment to identify mosquito species is the existence of morphologically identical sibling species that play different roles in the transmission of pathogens and parasites. Currently PCR diagnostics are used to distinguish between sibling species...
2013: PloS One
https://www.readbyqxmd.com/read/22917478/serum-proteome-changes-in-dengue-virus-infected-patients-from-a-dengue-endemic-area-of-india-towards-new-molecular-targets
#18
Sandipan Ray, Rajneesh Srivastava, Karnika Tripathi, Vineet Vaibhav, Swati Patankar, Sanjeeva Srivastava
The global burden of dengue continues to worsen, specifically in tropical and subtropical countries, and has evolved as a major public health problem. We investigated the changes in serum proteome in dengue fever (DF) patients from a dengue-endemic area of India to obtain mechanistic insights about the disease pathogenesis, the host immune response, and identification of potential serum protein biomarkers of this infectious disease. In this study, serum samples from DF patients, healthy subjects, and patients with falciparum malaria (an infectious disease control) were investigated by 2D-DIGE in combination with MALDI-TOF/TOF MS...
October 2012: Omics: a Journal of Integrative Biology
https://www.readbyqxmd.com/read/22554907/serum-profiling-of-leptospirosis-patients-to-investigate-proteomic-alterations
#19
MULTICENTER STUDY
Rajneesh Srivastava, Sandipan Ray, Vineet Vaibhav, Kishore Gollapalli, Tulip Jhaveri, Santosh Taur, Snigdha Dhali, Nithya Gogtay, Urmila Thatte, Rapole Srikanth, Sanjeeva Srivastava
Leptospirosis is a zoonotic infectious disease of tropical, subtropical and temperate zones, which is caused by the pathogenic spirochetes of genus Leptospira. Although this zoonosis is generally not considered as fatal, the pathogen can eventually cause severe infection with septic shock, multi-organ failure and lethal pulmonary hemorrhages leading to mortality. In this study, we have performed a proteomic analysis of serum samples from leptospirosis patients (n=6), febrile controls (falciparum malaria) (n=8) and healthy subjects (n=18) to obtain an insight about disease pathogenesis and host immune responses in leptospiral infections...
December 5, 2012: Journal of Proteomics
https://www.readbyqxmd.com/read/22539315/cationic-detergents-enable-the-separation-of-membrane-proteins-of-plasmodium-falciparum-infected-erythrocytes-by-2d-gel-electrophoresis
#20
Stephan Philipp, Thomas Jakoby, Andreas Tholey, Ottmar Janssen, Matthias Leippe, Christoph Gelhaus
The intraerythrocytic stage of Plasmodium falciparum alters the characteristics of its host cell by exporting selected plasmodial proteins. Although it is clear that the physicochemical and immunobiological properties of the host cell are modulated during parasite development, the involved plasmodial proteins and their mode of action are not completely known. Using cetyltrimethylammonium bromide (CTAB) or benzyldimethyl-n-hexadecylammonium chloride (16-BAC) for the first dimension and SDS for the second dimension, we separated proteins from membranes of human erythrocytes and of erythrocytes infected with the malaria parasite P...
April 2012: Electrophoresis
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