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https://www.readbyqxmd.com/read/27914355/characterisation-of-fine-grained-tailings-from-a-marble-processing-plant-and-their-acute-effects-on-the-copepod-calanus-finmarchicus
#1
Julia Farkas, Dag Altin, Karen M Hammer, Kaja C Hellstrøm, Andy M Booth, Bjørn Henrik Hansen
Submarine tailing disposal (STD) of mining waste is practiced as an alternative to land fill disposal in several countries. Knowledge regarding the environmental implications of STD on fjord and other marine ecosystems, including the pelagic environment, is scarce. In this study, we characterised the particle shape, size and metal content of the fine-grained fraction of tailings (FGT) from a Norwegian marble processing plant and investigated their acute toxicity and impact on feeding rate in adult Calanus finmarchicus...
November 30, 2016: Chemosphere
https://www.readbyqxmd.com/read/27913269/the-structure-organisation-and-perioperative-management-of-ambulatory-surgery-and-anaesthesia-in-france-methodology-of-the-sfar-opera-study
#2
Pierre Albaladejo, Frédéric Aubrun, Charles-Marc Samama, Laurent Jouffroy, Marc Beaussier, Dan Benhamou, Pauline Romegoux, Kristina Skaare, Jean Luc Bosson, Claude Ecoffey
The organization of health care establishments and perioperative care are essential for ensuring the quality of care and safety of patients undergoing outpatient surgery. In order to correctly inventory these organizations and practices, in 2013-2014, the French Society of Anaesthesia and Intensive Care organized an extensive practical survey in French ambulatory surgery units entitled the "OPERA" study (Organisation PERiopératoire de l'anesthésie en chirurgie Ambulatoire). From among all of the ambulatory surgery centres listed by the Agences Régionales de Santé [Regional Health Agencies, France], 206 public and private centres were randomly selected...
November 29, 2016: Anaesthesia, Critical Care & Pain Medicine
https://www.readbyqxmd.com/read/27913222/human-liver-regeneration-in-advanced-cirrhosis-is-organized-by-the-portal-tree
#3
Katalin Dezső, András Rókusz, Edina Bugyik, Armanda Szücs, András Szuák, Bence Dorogi, Mátyás Kiss, Ágnes Nemeskéri, Péter Nagy, Sándor Paku
BACKGROUND&AIMS: In advanced cirrhosis new hepatocytic nodules are generated by budding of ductules in areas of parenchymal extinction. However, the vascular alterations in the areas of parenchymal extinction, the blood supply and the structure of the new hepatocytic nodules have not been analyzed in detail. METHODS: Explanted human cirrhotic livers of three different etiologies and two experimental models of cirrhosis in rats were examined thoroughly. 3D reconstruction of the immunohistochemically stained serial sections and casting of human and experimental cirrhotic livers have been used to reveal the structural organization of the regenerative buds...
November 29, 2016: Journal of Hepatology
https://www.readbyqxmd.com/read/27906168/immunofluorescent-antibody-staining-of-intact-drosophila-larvae
#4
Laurina Manning, Chris Q Doe
Antibody staining is a vital technique for studying the development of many model organisms, including Drosophila. Reliable protocols have long been available for antibody staining of Drosophila whole-mount embryos and dissected larvae. By contrast, methods for staining whole larvae have rarely been reported, are unreliable, and fail to work on large third-instar larvae. This has become a major limitation to understanding the role of multitissue interactions such as neural circuit formation and cell metastasis...
January 2017: Nature Protocols
https://www.readbyqxmd.com/read/27905538/potential-and-optimization-of-two-phase-anaerobic-digestion-of-oil-refinery-waste-activated-sludge-and-microbial-community-study
#5
Qinghong Wang, Ying Liang, Peng Zhao, Qing X Li, Shaohui Guo, Chunmao Chen
Oil refinery waste activated sludge produced from oil wastewater biological treatment is a major industrial sludge. Two-phase anaerobic digestion of oil refinery waste activated sludge was studied for the first time. Thermal pretreatment under 170 °C is effective on sludge solubilization. At the optimum hydrolytic-acidogenic condition which was pH of 6.5, temperature of 55 °C and HRT of 2 days, 2754 mg/L volatile fatty acids (VFAs) were produced and acetic acid and butyric acid were the key components...
December 1, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27903656/yeast-hmo1-linker-histone-reinvented
#6
REVIEW
Arvind Panday, Anne Grove
Eukaryotic genomes are packaged in chromatin. The higher-order organization of nucleosome core particles is controlled by the association of the intervening linker DNA with either the linker histone H1 or high mobility group box (HMGB) proteins. While H1 is thought to stabilize the nucleosome by preventing DNA unwrapping, the DNA bending imposed by HMGB may propagate to the nucleosome to destabilize chromatin. For metazoan H1, chromatin compaction requires its lysine-rich C-terminal domain, a domain that is buried between globular domains in the previously characterized yeast Saccharomyces cerevisiae linker histone Hho1p...
March 2017: Microbiology and Molecular Biology Reviews: MMBR
https://www.readbyqxmd.com/read/27901501/identification-and-characterization-of-peptidases-secreted-by-quahog-parasite-unknown-qpx-the-protistan-parasite-of-hard-clams
#7
Ewelina Rubin, Glenn T Werneburg, Emmanuelle Pales Espinosa, David G Thanassi, Bassem Allam
Quahog parasite unknown (QPX) is a protistan parasite capable of causing deadly infections in the hard clam Mercenaria mercenaria, one of the most valuable shellfish species in the USA. QPX is an extracellular parasite found mostly in the connective tissue of clam mantle and, in more severe cases of infection, other clam organs. Histopathologic examinations revealed that QPX cells within clam tissues are typically surrounded by hollow areas that have been hypothesized to be, at least in part, a result of extracellular digestion of clam proteins by the parasite...
November 22, 2016: Diseases of Aquatic Organisms
https://www.readbyqxmd.com/read/27900432/novel-boronate-material-affords-efficient-enrichment-of-glycopeptides-by-synergized-hydrophilic-and-affinity-interactions
#8
Jianying Chen, Xiaohu Li, Mengyu Feng, Kun Luo, Juan Yang, Bo Zhang
Development of novel materials for enrichment of glycopeptides is the key to a comprehensive analysis of the glycoproteome, which is closely related to several major diseases and biomarker findings. We synthesized phenylboronic acid (PBA) bound to SiO2 microspheres by a thiol-ene click chemistry method (this material was denoted as click PBA) and used it to separate cis-diol-containing molecules and enrich glycopeptides in hydrophilic interaction chromatography mode. Successful preparation of click PBA was confirmed by elemental analysis, X-ray photoelectron spectroscopy, N2 adsorption-desorption isotherms, and high-resolution scanning electron microscopy...
November 29, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27899919/fermentation-of-ammonia-fiber-expansion-treated-and-untreated-barley-straw-in-a-rumen-simulation-technique-using-rumen-inoculum-from-cattle-with-slow-versus-fast-rate-of-fiber-disappearance
#9
Candace L Griffith, Gabriel O Ribeiro, Masahito Oba, Tim A McAllister, Karen A Beauchemin
The purpose of this study was to determine the effect of rumen inoculum from heifers with fast vs. slow rate of in situ fiber digestion on the fermentation of complex versus easily digested fiber sources in the forms of untreated and Ammonia Fiber Expansion (AFEX) treated barley straw, respectively, using an artificial rumen simulation technique (Rusitec). In situ fiber digestion was measured in a previous study by incubating untreated barley straw in the rumen of 16 heifers fed a diet consisting of 700 g/kg barley straw and 300 g/kg concentrate...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27898932/performance-and-methane-emissions-of-grazing-nellore-bulls-supplemented-with-crude-glycerin
#10
E San Vito, J F Lage, J D Messana, E E Dallantonia, R T S Frighetto, R A Reis, A J Neto, T T Berchielli
Supplementation of grass-fed cattle with low-cost feeding alternatives may be an attractive way to improve efficiency of cattle production. We hypothesized that inclusion of crude glycerin (CG) in supplements provided to grass-fed cattle could improve feed conversion without negative effects on growth performance while reducing methane emissions. Our hypothesis was tested using Nellore bulls grazing tropical pasture ( = 50; initial BW of 427 ± 19.41 kg; age of 17 ± 2 mo) supplemented with increasing concentrations (0, 70, 140, 210, and 280 g/kg DM basis of supplement) of CG and corn gluten replacing corn grain...
November 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898922/influence-of-pork-and-pork-by-products-on-macronutrient-and-energy-digestibility-and-palatability-in-large-exotic-felids
#11
C J Iske, C L Morris, K L Kappen
Two experiments were conducted to evaluate digestibility and palatability of a new commercial pork-based raw diet for zoo-managed felids. Currently 2 protein sources (beef or horse) comprise the majority of commercial raw meat diet formulations for exotic carnivores in zoological institutions. Pork-based diets have traditionally not been widely utilized and thus nutrient digestibility of pork has not been adequately evaluated in exotic carnivores. The objectives of this study were 1) to determine if a pork-based diet had similar apparent total tract macronutrient digestibility and fecal scores as standard zoo carnivore diets formulated with either horse or beef, in large exotic felids and 2) evaluate palatability of pork for use in zoos...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898898/effects-of-molasses-and-crude-glycerol-combined-in-a-liquid-supplement-on-ruminal-fermentation-in-beef-steers-consuming-bermudagrass-hay
#12
F M Ciriaco, D D Henry, V R G Mercadante, T M Schulmeister, M Ruiz-Moreno, G C Lamb, N DiLorenzo
Two experiments were performed to evaluate the effects of 1) increasing supplementation doses of a 50:50 (as-fed) liquid supplement of molasses and crude glycerol (M:G) on ruminal fermentation parameters and blood urea nitrogen (BUN) in beef steers consuming Tifton 85 bermudagrass ( spp.) hay and 2) different proportions of molasses and crude glycerol in a liquid supplement on in vitro fermentation and gas production kinetics. For Exp. 1, 8 ruminally cannulated, Angus-crossbred steers were used in a duplicated 4 × 4 Latin square design, had ad libitum access to Tifton 85 bermudagrass hay, and were randomly assigned to 1 of 4 treatments: 1) CTRL, no supplementation; 2) SUP1, 0...
September 2016: Journal of Animal Science
https://www.readbyqxmd.com/read/27898741/stable-host-gene-expression-in-the-gut-of-adult-drosophila-melanogaster-with-different-bacterial-mono-associations
#13
Carolyn Elya, Vivian Zhang, William B Ludington, Michael B Eisen
There is growing evidence that the microbes found in the digestive tracts of animals influence host biology, but we still do not understand how they accomplish this. Here, we evaluated how different microbial species commonly associated with laboratory-reared Drosophila melanogaster impact host biology at the level of gene expression in the dissected adult gut and in the entire adult organism. We observed that guts from animals associated from the embryonic stage with either zero, one or three bacterial species demonstrated indistinguishable transcriptional profiles...
2016: PloS One
https://www.readbyqxmd.com/read/27898321/anaerobic-batch-conversion-of-pine-wood-torrefaction-condensate
#14
Tharaka Rama Krishna C Doddapaneni, Ramasamy Praveenkumar, Henrik Tolvanen, Marja R T Palmroth, Jukka Konttinen, Jukka Rintala
Organic compound rich torrefaction condensate, owing to their high water content and acidic nature, have yet to be exploited for practical application. In this study, microbial conversion of torrefaction condensate from pine wood through anaerobic batch digestion (AD) to produce methane was evaluated. Torrefaction condensate exhibited high methane potentials in the range of 430-492mL/g volatile solids (VS) and 430-460mL/gVS under mesophilic and thermophilic conditions, respectively. Owing to the changes in the composition, the methane yields differed with the torrefaction condensates produced at different temperatures (225, 275 and 300°C), with a maximum of 492±18mL/gVS with the condensate produced at 300°C under mesophilic condition...
November 22, 2016: Bioresource Technology
https://www.readbyqxmd.com/read/27896609/moral-polemics-of-far-reaching-economic-consequences-of-antibiotics-overuse
#15
Marek Vochozka, Anna Maroušková, Petr Šuleř
The unethical overuse of antibiotics to seek to achieve a shortening of the treatment period raises the cost of health services and poses a threat to humanity due to the gradual development of antibiotic resistance. Other consequences of our modern passion for antibiotics have appeared. Small concentrations of antibiotic residues in sewage waters slow down the metabolism of anaerobic microorganism thereby reducing the overall performance of the anaerobic fermentation used to detoxify and digest sewage and other collected organic wastes...
November 28, 2016: Science and Engineering Ethics
https://www.readbyqxmd.com/read/27895742/comparative-analysis-of-gene-expression-profiles-of-gastric-cardia-adenocarcinoma-and-gastric-non-cardia-adenocarcinoma
#16
Bin Song, Juan Du, Neng Deng, Ji-Chen Ren, Zhen-Bo Shu
In the present study, gene expression profiles were analyzed to identify the molecular mechanisms underlying gastric cardia adenocarcinoma (GCA) and gastric non-cardia adenocarcinoma (GNCA). A gene expression dataset (accession number GSE29272) was downloaded from Gene Expression Omnibus, and consisted of 62 GCA samples and 62 normal controls, as well as 72 GNCA samples and 72 normal controls. The two groups of differentially-expressed genes (DEGs) were compared to obtain common and unique DEGs. A differential analysis was performed using the Linear Models for Microarray Data package in R...
November 2016: Oncology Letters
https://www.readbyqxmd.com/read/27895707/fungal-fermentation-on-anaerobic-digestate-for-lipid-based-biofuel-production
#17
Yuan Zhong, Zhiguo Liu, Christine Isaguirre, Yan Liu, Wei Liao
BACKGROUND: Anaerobic digestate is the effluent from anaerobic digestion of organic wastes. It contains a significant amount of nutrients and lignocellulosic materials, even though anaerobic digestion consumed a large portion of organic matters in the wastes. Utilizing the nutrients and lignocellulosic materials in the digestate is critical to significantly improve efficiency of anaerobic digestion technology and generate value-added chemical and fuel products from the organic wastes...
2016: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/27895594/larval-exposure-to-chlorpyrifos-affects-nutritional-physiology-and-induces-genotoxicity-in-silkworm-philosamia-ricini-lepidoptera-saturniidae
#18
Moni K Kalita, Kishor Haloi, Dipali Devi
Chlorpyrifos is a most widely used organophosphate insecticide because of its cost effectiveness and degradable nature. However, this pesticide enters and contaminates the environment either by direct application, spray drifts or crop run off and shows adverse effect on the non-targeted organisms. Philosamia ricini (eri silkworm), one of the most exploited, domesticated and commercialized non mulberry silkworm is known for mass production of eri silk. The silkworm larvae get exposed to pesticide residues on the leaves of food plants...
2016: Frontiers in Physiology
https://www.readbyqxmd.com/read/27894769/identification-of-molecular-species-of-monoalkyldiacylglycerol-from-the-squid-berryteuthis-magister-using-liquid-chromatography-apci-high-resolution-mass-spectrometry
#19
Viacheslav G Rybin, Andrey B Imbs, Darja A Demidkova, Ekaterina V Ermolenko
Monoalkyldiacylglycerol (MADAG) is an important lipid class in mollusks, corals, starfishes, and some species of zooplankton. Up to 80% of liver oils of squids and sharks are comprised of MADAG. Except for one fish species, there are no data on the composition of MADAG molecular species of marine organisms. The molecular species of MADAG obtained from digestive glands of the deep-sea squid Berryteuthis magister were identified. High-performance liquid chromatography (HPLC) and tandem high-resolution mass spectrometry (HRMS) with atmospheric pressure chemical ionization (APCI) were applied...
November 25, 2016: Chemistry and Physics of Lipids
https://www.readbyqxmd.com/read/27894055/indirect-effects-of-climate-changes-on-cadmium-bioavailability-and-biological-effects-in-the-mediterranean-mussel-mytilus-galloprovincialis
#20
Alessandro Nardi, Luana Fiorella Mincarelli, Maura Benedetti, Daniele Fattorini, Giuseppe d'Errico, Francesco Regoli
Despite the great interest in the consequences of climate change on the physiological functioning of marine organisms, indirect and interactive effects of rising temperature and pCO2 on bioaccumulation and responsiveness to environmental pollutants are still poorly explored, particularly in terms of cellular mechanisms. According to future projections of temperature and pH/pCO2, this study investigated the main cellular pathways involved in metal detoxification and oxidative homeostasis in Mediterranean mussels, Mytilus galloprovincialis, exposed for 4 weeks to various combinations of two levels of pH/pCO2 (8...
November 25, 2016: Chemosphere
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