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Fernanda B Campagnollo, Larissa P Margalho, Bruna A Kamimura, Marcelo D Feliciano, Luisa Freire, Letícia S Lopes, Verônica O Alvarenga, Vasco A P Cadavez, Ursula Gonzales-Barron, Donald W Schaffner, Anderson S Sant'Ana
Artisanal raw milk cheeses are highly appreciated dairy products in Brazil and ensuring their microbiological safety has been a great need. This study reports the isolation and characterization of lactic acid bacteria (LAB) strains with anti-listerial activity, and their effects on Listeria monocytogenes during refrigerated shelf-life of soft Minas cheese and ripening of semi-hard Minas cheese. LAB strains (n = 891) isolated from Minas artisanal cheeses (n = 244) were assessed for anti-listerial activity by deferred antagonism assay at 37 °C and 7 °C...
August 2018: Food Microbiology
Sanye Soroldoni, Ítalo Braga Castro, Fiamma Abreu, Fabio Andrei Duarte, Rodrigo Brasil Choueri, Osmar Olinto Möller, Gilberto Fillmann, Grasiela Lopes Leães Pinho
Sources, occurrence, composition and dynamics of antifouling paint particles (APPs) were assessed in Patos Lagoon estuary (PLE), Southern Brazil. Ten areas including boatyards, a marina and artisanal fishing harbors were identified in the estuarine system as potential sources of APPs. The APPs generated in these areas were highly heterogeneous considering the size, shape and composition. Based on an estimate of antifouling paint usage and amount of boats in each studied area, artisanal fishing harbors could be the main source of particles to PLE...
March 2, 2018: Water Research
Ludmila Vilela Resende, Leticia Kleinhans Pinheiro, Maria Gabriela da Cruz Pedroso Miguel, Cíntia Lacerda Ramos, Danielle Marques Vilela, Rosane Freitas Schwan
The microbial community of artisanal corn fermentation called Chicha were isolated, purified and then identified using protein profile by Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF) and confirmed by partial ribosomal gene sequencing. Samples from Chicha beverage were chemically characterized by gas and liquid chromatography (HPLC and GC-MS). Aerobic mesophilic bacteria (AMB) (35.8% of total of isolated microorganisms), lactic acid bacteria (LAB) (21.6%) and yeast (42...
March 8, 2018: World Journal of Microbiology & Biotechnology
B González-García, S García-Isidoro, J Contreras-Alcántara, J A Martínez-Gil, M A Díaz Y Orea, V O Castellanos-Sánchez
The most frequent diabetes-related complications are diabetic foot and glaucoma, which lead to amputation and loss of vision, respectively. Current scientific and technologic developments have permitted the design and implementation of prosthetic systems that are optimal for these patients, as the latter adapt themselves to them and can resume activities of daily living. The lack of economic resources compromises the quality of the prostheses patients can afford, as they resort to «artisanal» or «rustic» systems that hamper their adaptation process...
September 2017: Acta Ortopédica Mexicana
F Owusu-Nimo, J Mantey, K B Nyarko, Eugene Appiah-Effah, A Aubynn
Recently, there have been efforts by stakeholders to monitor illegal mining ( galamsey) activities, foster their formalization and reclaim the many abandoned wastelands in Ghana. However, limited information exists on the locations, abundance, scope and scale of galamsey types, which hinders the development of effective policy response. This study attempts to map and analyze the distribution patterns, abundance, activity statuses and the extents of nine (9) galamsey types within eleven (11) Municipal and District Assemblies (MDAs) of Ghana's Western Region...
February 2018: Heliyon
Carlos Eduardo Serrano-Maldonado, Israel García-Cano, Augusto González-Canto, Eliel Ruiz-May, Jose Miguel Elizalde-Contreras, Maricarmen Quirasco
The atlD gene from an Enterococcus faecalis strain isolated from a Mexican artisanal cheese was cloned, sequenced and expressed in Escherichia coli in order to perform a biochemical characterization. A partial amino acid sequence of the heterologous protein was obtained by LC-MS/MS, and it corresponded to a novel peptidoglycan hydrolase designated AtlD. Its molecular mass was 62-75 kDa, as determined by SDS-PAGE, zymography, Western blot, and exclusion chromatography. Electrofocusing rendered an isoelectric point (pI) of 4...
March 6, 2018: Journal of Molecular Microbiology and Biotechnology
Jorge de Jesus Tirado-Ibarra, Mariany Loya-Rodriguez, Jose Carlos Morales-Arevalo, Isabel Rosario Muñoz-Garcia, Francisco Martinez-Perez, Jorge Saul Ramirez-Perez, Laura Rebeca Jimenez-Gutierrez
The shrimp fishery is one of the most important fisheries in the world, although the low selectivity from trawling nets has led to the capture of a large number of non-target species. Shrimp-bycatch species include a large number of fish and invertebrate species, of which fish species are the most abundant. The present study aims to determine the community structure as well as the average sizes at first maturity of the fish species from shrimp-bycatch caught from industrial fisheries in the Mexican Pacific from Sinaloa to Guerrero, from January to March 2015...
2018: PeerJ
Anjali Kumar, Timothy J Divoll, Priya M Ganguli, Florencia A Trama, Carl H Lamborg
Mercury, a toxic trace metal, has been used extensively as an inexpensive and readily available method of extracting gold from fine-grained sediment. Worldwide, artisanal mining is responsible for one third of all mercury released into the environment. By testing bat hair from museum specimens and field collected samples from areas both impacted and unimpacted by artisanal gold mining in Perú, we show monomethylmercury (MMHg) has increased in the last 100 years. MMHg concentrations were also greatest in the highest bat trophic level (insectivores), and in areas experiencing extractive artisanal mining...
February 20, 2018: Environmental Pollution
José F Domínguez-Contreras, Adrian Munguia-Vega, Bertha P Ceballos-Vázquez, Marcial Arellano-Martínez, Francisco J García-Rodríguez, Melanie Culver, Hector Reyes-Bonilla
The fishery for octopus in Northwest Mexico has increased to over 2,000 tons annually, but to date the specific composition of the catch has been ignored. With at least three main species targeted by artisanal fisheries in the region with distinct life histories, the lack of basic biological information about the distribution, metapopulation size and structure of each species could impede effective fisheries management to avoid overexploitation. We tested if different life histories of three species of octopus could help predict observed patterns of genetic diversity, population dynamics, structure and connectivity and how this information could be relevant to the sustainable management of the fishery...
2018: PeerJ
Juan Gassman, Héctor López Rojas, Dana Padrón
Snooks C. undecimalis and C. ensiferus are important fishery resources in their distribution area. In Venezuela they are caught by artisanal fisheries along the entire coast. However, studies on their biology that would help establish fishing regulations are scarce. To study reproduction aspects of these species, we analyzed 1 325 individuals of C. undecimalis and 2 025 of C. ensiferus caught by artisanal fishing in the Laguna de Tacarigua in Northern Venezuela, from July 2009 to September 2010. Total length, total weight, sex and stage of gonadal development were recorded...
March 2017: Revista de Biología Tropical
Maria João Cruz, Miguel Machete, Gui Menezes, Emer Rogan, Mónica A Silva
Small-scale artisanal fisheries can have a significant negative impact in cetacean populations. Cetacean bycatch has been documented in the pole-and-line tuna fishery in the Azores with common dolphins being the species more frequently taken. Based on data collected by observers on ∼50% of vessels operating from 1998 to 2012, we investigate the influence of various environmental and fisheries-related factors in common dolphin bycatch and calculate fleet-wide estimates of total bycatch using design-based and model-based methods...
2018: PeerJ
M Bergamaschi, G Bittante
The role of each step of cheese and ricotta making in development of flavor of cheese and other dairy products is not yet well known. The objectives of this study were to characterize volatile organic compounds (VOC) in cheese and ricotta making with bulk milk from cows grazing in a highland area and to evaluate their evolution in the various dairy products and by-products obtained during the production processes. A group of 148 cows was grazed day and night on pasture from June to September. A total of 7 cheese-making sessions were carried out using the bulk milk collected every 2 wk during summer pasturing according to the artisanal procedure used for Malga cheese production...
February 14, 2018: Journal of Dairy Science
Esteban Marcellin, Lars Keld Nielsen
The emergence of inexpensive, base-perfect genome editing is revolutionising biology. Modern industrial biotechnology exploits the advances in genome editing in combination with automation, analytics and data integration to build high-throughput automated strain engineering pipelines also known as biofoundries. Biofoundries replace the slow and inconsistent artisanal processes used to build microbial cell factories with an automated design-build-test cycle, considerably reducing the time needed to deliver commercially viable strains...
February 12, 2018: Current Opinion in Biotechnology
Xiaoji Zheng, Fei Liu, Xuewei Shi, Bin Wang, Kaixiong Li, Baokun Li, Bin Zhuge
Cheese is a typical handcrafted fermented food in Kazak minority from the Uighur Autonomy Region in China. The ripening process of the cheese is crucial for quality and flavor. The aim of this study was to gain a deeper knowledge on the bacterial and fungal community diversity at different time points during the post-ripening of the cheese and to understand the relationship between bacterial and fungal profiles and the chemical components including amino acids, fatty acids and volatile compounds related the cheese flavor...
March 2018: Food Research International
María A Correa Deza, Gladys I Martos, Marta Nuñez, Mario Fiori, Carla L Gerez, Graciela Font
In artisanal tanneries, the skins are immersed in cereals fermented by natural microbial flora in order to reduce the pH of the skin, an essential condition for carrying out the final step. The environmental thermal variation alters the fermentation process and affects the quality of the final product. The aim of this work was to isolate lactic acid bacteria from cereals mixture fermented in an artisanal tannery and to evaluate in vitro the acidifying activity of the strains as a first step for the formulation of a starter culture...
February 12, 2018: Journal of Basic Microbiology
Giulia S Porcari, Cary Fu, Emily D Doll, Emma G Carter, Robert P Carson
Medically refractory epilepsy continues to be a challenge worldwide, and despite an increasing number of medical therapies, approximately 1 in 3 patients continues to have seizures. Cannabidiol (CBD), one of many constituents of the Cannabis sativa or marijuana plant, has received renewed interest in the treatment of epilepsy. While highly purified CBD awaits Food and Drug Administration (FDA) approval, artisanal formulations of CBD are readily available and are seeing increased use in our patient population...
February 8, 2018: Epilepsy & Behavior: E&B
Zhaoxu Meng, Lanwei Zhang, Liang Xin, Kai Lin, HuaXi Yi, Xue Han
The potential of 25 Lactobacillus isolates from 8 semihard artisanal goat cheeses manufactured in 4 different Mediterranean areas was examined for use as nonstarter lactic acid bacteria. The isolates were identified using 16S rDNA sequence analysis. Sixteen strains belonged to Lactobacillus paracasei and 9 to Lactobacillus rhamnosus. The isolates were first screened for salt tolerance, exopolysaccharide and diacetyl production, proteolytic and lipolytic activity, and acidification and autolyzing capacities...
February 7, 2018: Journal of Dairy Science
Derrick Risner, Avi Shayevitz, Karl Haapala, Lisbeth Meunier-Goddik, Paul Hughes
Whey disposal can be both an environmental and economic challenge for artisanal creameries. Lactose in whey can be fermented to produce ethanol and subsequently distilled. The objective of this study was to use a process-based life cycle analysis to compare carbon dioxide-equivalent (CO2e) emissions and water usage associated with the artisanal or craft production of clear, unaged spirits using whey or malted barley as fermentation substrate. Differences in production were assessed based on key process differences: energy used, water used, distillation by-product disposal, and mass of CO2 produced during fermentation...
February 2, 2018: Journal of Dairy Science
S Johler, G Macori, A Bellio, P L Acutis, S Gallina, L Decastelli
Staphylococcus aureus is a common cause of food-borne intoxications. Several staphylococcal food poisoning outbreaks have been linked to consumption of raw milk cheeses and artisanal cheese production. However, information on Staph. aureus isolated from artisanal raw milk cheeses and small-scale dairy production environments is very limited. Therefore, we aimed to characterize Staph. aureus isolated along the artisanal raw milk production chain by determining (1) the population structure, and (2) the presence/absence of enterotoxin genes, mecA/C, and pvl...
January 31, 2018: Journal of Dairy Science
Heileen Hsu-Kim, Chris S Eckley, Dario Achá, Xinbin Feng, Cynthia C Gilmour, Sofi Jonsson, Carl P J Mitchell
The environmental cycling of mercury (Hg) can be affected by natural and anthropogenic perturbations. Of particular concern is how these disruptions increase mobilization of Hg from sites and alter the formation of monomethylmercury (MeHg), a bioaccumulative form of Hg for humans and wildlife. The scientific community has made significant advances in recent years in understanding the processes contributing to the risk of MeHg in the environment. The objective of this paper is to synthesize the scientific understanding of how Hg cycling in the aquatic environment is influenced by landscape perturbations at the local scale, perturbations that include watershed loadings, deforestation, reservoir and wetland creation, rice production, urbanization, mining and industrial point source pollution, and remediation...
January 31, 2018: Ambio
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