Read by QxMD icon Read


Selwyn Arlington Headley, Lucienne Garcia Pretto-Giordano, Giovana Wingeter Di Santis, Lucas Alécio Gomes, Rafaela Macagnan, Daniela Farias da Nóbrega, Katherine Moura Leite, Brígida Kussumoto de Alcântara, Eiko Nakagawa Itano, Amauri Alcindo Alfieri, Mario Augusto Ono
Paracoccidioidomycosis (PCM) is an endemic disease of humans from Latin America that is caused by Paracoccidioides brasiliensis and P. lutzii, with most cases of PCM in domestic animals being associated with P. brasiliensis. This study presents the clinical, cytological, mycological, serological, and molecular findings associated with P. brasiliensis in a dog from Southern Brazil. Fine needle biopsies were collected from the skin and several lymph nodes of a 5-year-old female Labrador dog that had enlargement of most superficial lymph nodes...
October 18, 2016: Mycopathologia
Thiago Alves da Costa, Rosária Di Gangi, Rodolfo Thomé, Marina Barreto Felisbino, Amanda Pires Bonfanti, Larissa Lumi Watanabe Ishikawa, Alexandrina Sartori, Eva Burger, Liana Verinaud
T cell maturation takes place within the thymus, a primary lymphoid organ that is commonly targeted during infections. Previous studies showed that acute infection with Paracoccidioides brasiliensis (Pb), the causative agent of paracoccidioidomycosis (PCM), promotes thymic atrophy that is associated with the presence of yeast cells in the organ. However, as human PCM is a chronic infection, it is imperative to investigate the consequences of Pb infection over the thymic structure and function in chronic infection...
2016: PloS One
Sérgio Monteiro de Almeida, Thiago Henrique Roza
The immune reconstitution inflammatory syndrome (IRIS) is a deregulated inflammatory response to invading microorganisms. It is manifested when there is an abrupt change in host immunity from an anti-inflammatory and immunosuppressive state to a pro-inflammatory state as a result of rapid depletion or removal of factors that promote immune suppression or inhibition of inflammation. The aim of this paper is to discuss and re-interpret the possibility of association of paracoccidioidomycosis (PCM) with IRIS in the central nervous system (CNS) in a case from Brazil published by Silva-Vergara ML...
October 7, 2016: Mycopathologia
José F Muñoz, Rhys A Farrer, Christopher A Desjardins, Juan E Gallo, Sean Sykes, Sharadha Sakthikumar, Elizabeth Misas, Emily A Whiston, Eduardo Bagagli, Celia M A Soares, Marcus de M Teixeira, John W Taylor, Oliver K Clay, Juan G McEwen, Christina A Cuomo
The Paracoccidioides genus includes two species of thermally dimorphic fungi that cause paracoccidioidomycosis, a neglected health-threatening human systemic mycosis endemic to Latin America. To examine the genome evolution and the diversity of Paracoccidioides spp., we conducted whole-genome sequencing of 31 isolates representing the phylogenetic, geographic, and ecological breadth of the genus. These samples included clinical, environmental and laboratory reference strains of the S1, PS2, PS3, and PS4 lineages of P...
September 2016: MSphere
Juan David Puerta-Arias, Paula Andrea Pino-Tamayo, Julián Camilo Arango, Ángel González
Chronic stages of paracoccidioidomycosis (PCM) are characterized by granulomatous lesions which promote the development of pulmonary fibrosis leading to the loss of respiratory function in 50% of patients; in addition, it has been observed that neutrophils predominate during these chronic stages of P. brasiliensis infection. The goal of this study was to evaluate the role of the neutrophil during the chronic stages of experimental pulmonary PCM and during the fibrosis development and tissue repair using a monoclonal specific to this phagocytic cell...
2016: PloS One
Lucas G G Cabrera, Aline F Santos, Ursulla V Andrade, Carlos Ivan A Guedes, Sandra M V L Oliveira, Marilene R Chang, Rinaldo P Mendes, Anamaria M M Paniago
Paracoccidioidomycosis (PCM) is the most important systemic mycosis in Latin America. About 80% of PCM patients are present with its chronic form. The lungs are affected in most patients with the chronic form; however, pleural involvement has rarely been reported. We describe nine cases of PCM that presented with lung involvement and spontaneous pneumothorax. All patients, except one whose condition was not investigated, were smokers. PCM was diagnosed during the pneumothorax episode in three patients, and from 3 to 16 years before the pneumothorax episode in six patients...
September 30, 2016: Mycoses
Lucas Nojosa Oliveira, Luciana Casaletti, Sônia Nair Báo, Clayton Luiz Borges, Patrícia de Sousa Lima, Célia Maria de Almeida Soares
Paracoccidioidomycosis is an endemic disease in Latin America, caused by thermo dimorphic fungi of the genus Paracoccidioides. Although previous proteome analyses of Paracoccidioides spp. have been carried out, the nuclear subproteome of this pathogen has not been described. In this way, we aimed to characterize the nuclear proteome of Paracoccidioides species, in the yeast form. For that, yeast cells were disrupted and submitted to cell fractionation. The purity of the nuclear fraction was confirmed by fluorescence and electron microscopy...
October 2016: Fungal Biology
Paula Andrea Pino-Tamayo, Juan David Puerta-Arias, Damaris Lopera, Martha Eugenia Urán-Jiménez, Ángel González
Neutrophils predominate during the acute phase of the Paracoccidioides brasiliensis infection. Herein, we determined the role of the neutrophil during the early stages of experimental pulmonary paracoccidioidomycosis using a monoclonal antibody (mAb) specific for neutrophils. Male BALB/c mice were inoculated intranasally with 1.5 × 10(6) or 2 × 10(6) P. brasiliensis yeast cells. The mAb was administered 24 h before infection, followed by doses every 48 h until mice were sacrificed. Survival time was evaluated and mice were sacrificed at 48 h and 96 h after inoculation to assess cellularity, fungal load, cytokine/chemokine levels, and histopathological analysis...
2016: Mediators of Inflammation
Taise Natali Landgraf, Fabrício Freitas Fernandes, Gabriela Peron, Ademilson Panunto-Castelo
The lack of antifungals with low toxicity and short-term therapy for patients with paracoccidioidomycosis (PCM) led us to evaluate adjuvants in immunotherapeutic intervention. We have previously shown complete Freund's adjuvant (CFA) to be therapeutic on experimental PCM. Owing to CFA toxicity, here we tested adjuvants approved for clinical use or in preclinical phase in experimental mouse PCM. Of all, only monophosporyl lipid A (MPLA) demonstrates a beneficial effect, by reducing the fungal burden and increasing the concentrations of IFN-γ and TNF-α, which are immunoprotective in PCM...
September 17, 2016: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
Caroline Maria Marcos, Haroldo Cesar de Oliveira, Julhiany de Fátima da Silva, Patricia Akemi Assato, Daniella Sayuri Yamazaki, Rosângela Aparecida Moraes da Silva, Cláudia Tavares Santos, Norival Alves Santos-Filho, Deivys Leandro Portuondo, Maria José Soares Mendes-Giannini, Ana Marisa Fusco-Almeida
Paracoccidioides spp., which are temperature-dependent dimorphic fungi, are responsible for the most prevalent human systemic mycosis in Latin America, the paracoccidioidomycosis. The aim of this study was to characterise the involvement of elongation factor Tu (EF-Tu) in Paracoccidioides brasiliensis-host interaction. Adhesive properties were examined using recombinant PbEF-Tu proteins and the respective polyclonal anti-rPbEF-Tu antibody. Immunogold analysis demonstrated the surface location of EF-Tu in P...
November 2016: FEMS Yeast Research
Paulo Mendes Peçanha, Stella de Souza, Aloísio Falqueto, Tânia Regina Grão-Veloso, Ludmila Ventura Lírio, Carlos Urbano Gonçalves Ferreira, Aline Rocha Santos, Hélbia Garcia Costa, Lúcia Renata Meirelles de Souza, Felipe Francisco Tuon
Amphotericin B deoxycholate is the main option for intravenous (i.v.) treatment of severe paracoccidioidomycosis (PCM). This is the first report of amphotericin B lipid complex (ABLC) in the treatment of PCM. Among 28 patients, cure was achieved in all patients (100%) using ABLC. Mean and median daily doses of ABLC were 3.39 mg/kg/day and 3.35 mg/kg/day, respectively. ABLC may be a choice in the treatment of severe forms of PCM or when i.v. options are required.
October 2016: International Journal of Antimicrobial Agents
Fabrício Freitas Fernandes, Leandro Licursi de Oliveira, Taise Natali Landgraf, Gabriela Peron, Marcelo Vieira Costa, Arlete A M Coelho-Castelo, Vânia L D Bonato, Maria-Cristina Roque-Barreira, Ademilson Panunto-Castelo
The genus Paracoccidioides comprises species of dimorphic fungi that cause paracoccidioidomycosis (PCM), a systemic disease prevalent in Latin America. Here, we investigated whether administration of native 60-kDa heat shock protein of P. brasiliensis (nPbHsp60) or its recombinant counterpart (rPbHsp60) affected the course of experimental PCM. Mice were subcutaneously injected with nPbHsp60 or rPbHsp60 emulsified in complete's Freund Adjuvant (CFA) at three weeks after intravenous injection of P. brasiliensis yeasts...
2016: PloS One
Fabiana Rocha-Silva, Luciana Inácia Gomes, Alfredo Miranda Góes, Cidiane Graciele-Melo, Rachel Basques Caligiorne
: Paracoccidioidomycosis (PCM) is a systemic mycosis caused by dimorphic fungi Paracoccidioides brasiliensis and P. lutzii. It is prevalent in Latin American, mainly in Brazil. Therefore, PCM has fundamental impact in the Brazilian global economy, especially in public health system, since it is affecting economical active population in different country regions. OBJECTIVE: The present study aimed to standardize the Real Time-Polymerase Chain Reaction (rt-PCR) for an efficient and safe PCM diagnosis by amplifying the recombinant protein PB27 gene, only expressed by specimens of Paracoccidioides genus...
September 5, 2016: Recent Patents on Endocrine, Metabolic & Immune Drug Discovery
Alison F A Chaves, Daniele G Castilho, Marina V Navarro, Ana K Oliveira, Solange M T Serrano, Alexandre K Tashima, Wagner L Batista
Paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in Latin America. This fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. Therefore, the survival of P. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. For several years, reactive oxygen species (ROS) were only associated with pathological processes...
August 30, 2016: Microbes and Infection
Rosana Puccia, Milene Carmes Vallejo, Larissa V G Longo
Paracoccidioides brasiliensis and P. lutzii cause human paracoccidioidomycosis (PCM). They are dimorphic ascomycetes that grow as filaments at mild temperatures up to 28°C and as multibudding pathogenic yeast cells at 37°C. Components of the fungal cell wall have an important role in the interaction with the host because they compose the cell outermost layer. The Paracoccidioides cell wall is composed mainly of polysaccharides, but it also contains proportionally smaller rates of proteins, lipids, and melanin...
August 12, 2016: Current Protein & Peptide Science
Vanessa Noal, Suelen Santos, Karen Spadari Ferreira, Sandro Rogerio Almeida
: Paracoccidioidomycosis is a systemic mycosis endemic to Latin America. The infection is initiated by inhalation of conidia into the lung and may develop as localized or disseminated disease depending on the depression of cellular immunity. In the present study, we observed that intratracheal infection with P.b. caused the percentage of resident B-1 cells to decrease. Using xid mice reconstituted with B-1 cells, we observed that B-1 cells migrate to the spleen and stimulate increases in the regulatory T cell subpopulation in response to P...
August 10, 2016: Microbes and Infection
J E Carrasco-Zuber, C Navarrete-Dechent, A Bonifaz, F Fich, V Vial-Letelier, D Berroeta-Mauriziano
In the second part of this review on the deep mycoses, we describe the main systemic mycoses-paracoccidioidomycosis, coccidioidomycosis, histoplasmosis, mucormycosis, and cryptococcosis-and their cutaneous manifestations. Skin lesions are only occasionally seen in deep systemic mycoses either directly, when the skin is the route of entry for the fungus, or indirectly, when the infection has spread from a deeper focus. These cutaneous signs are often the only clue to the presence of a potentially fatal infection...
August 4, 2016: Actas Dermo-sifiliográficas
Junya de Lacorte Singulani, Liliana Scorzoni, Ana Carolina Alves de Paula E Silva, Ana Marisa Fusco-Almeida, Maria José Soares Mendes-Giannini
Paracoccidioides brasiliensis and P. lutzii belong to a group of thermodimorphic fungi and cause paracoccidioidomycosis (PCM), which is a human systemic mycosis endemic in South and Central America. Patients with this mycosis are commonly treated with amphotericin B (AmB) and azoles. The study of fungal virulence and the efficacy and toxicity of antifungal drugs has been successfully performed in a Galleria mellonella infection model. In this work, G. mellonella larvae were infected with two Paracoccidioides spp...
September 2016: International Journal of Antimicrobial Agents
Silvio Alencar Marques, Joel Carlos Lastória, Rosangela Maria Pires de Camargo, Mariangela Esther Alencar Marques
The authors report aspects of paracoccidioidomycosis, acute-subacute clinical form, juvenile type, in a 19-year-old female patient. Paracoccidioidomycosis, juvenile type, classically occurs in young patients, both sexes, with lymphoma-like aspects as initial presentation. However, following the natural history of the disease the lymph nodes assume patterns of infectious disease, as an abscess and fistulae. Systemic dissemination of the disease can occur and lethality and morbidity are significant in this clinical presentation...
May 2016: Anais Brasileiros de Dermatologia
Cyntia Silva Ferreira, Erica Milena de Castro Ribeiro, Alfredo de Miranda Goes, Breno de Mello Silva
Paracoccidioidomycosis (PCM) is a human systemic granulomatous mycosis caused by thermodimorphic fungi from Paracoccidioides genus. The disease is prevalent in Latin America and triggers a serious clinical condition. Consequently, rapid diagnosis and treatment are crucial to prevent progression of the disease, which can result in death. Currently, there are several established methods for PCM diagnosis. However, many of these tests still present challenges in terms of cost, accessibility and efficiency. In this scenario, gold nanoparticles represent a promising alternative since they have particular optical and electronic properties, which allow its use for biomolecules detection...
July 2016: Future Microbiology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"