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Tissue specific protein interactions

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https://www.readbyqxmd.com/read/28737766/endothelial-fibrosis-induced-by-suppressed-stat3-expression-mediated-by-signaling-involving-the-tgf-%C3%AE-1-alk5-smad-pathway
#1
Alvaro Becerra, Macarena Rojas, Alejandro Vallejos, Vicente Villegas, Lorena Pérez, Claudio Cabello-Verrugio, Felipe Simon
During systemic inflammatory pathologies, mediators of inflammation circulate in the bloodstream and interact with endothelial cells (ECs), resulting in endothelial dysfunction that maintains and enhances the pathological condition. Inflammatory mediators change the protein expression profile of ECs, which become activated fibroblasts via endothelial-to-mesenchymal transition. This process is characterized by downregulated endothelial proteins and strongly upregulated fibrotic-specific genes and extracellular matrix-forming proteins...
July 24, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28733469/bone-morphogenetic-proteins-breast-cancer-and-bone-metastases-striking-the-right-balance
#2
Catherine Zabkiewicz, Jeyna Resaul, Rachel Hargest, Wen Jiang, Lin Ye
Bone morphogenetic proteins (BMPs) belong to the TGF-β super family, and are essential for regulation of foetal development, tissue differentiation and homeostasis, and a multitude of cellular functions. Naturally, this has led to the exploration of aberrance in this highly regulated system as a key factor in tumourigenesis. Originally identified for their role in osteogenesis and bone turnover, attention has been turned to the potential role of BMPs in tumour metastases to, and progression within, the bone niche...
July 21, 2017: Endocrine-related Cancer
https://www.readbyqxmd.com/read/28732675/substrate-stiffness-regulates-arterial-venous-differentiation-of-endothelial-progenitor-cells-via-the-ras-mek-pathway
#3
Changyue Xue, Tao Zhang, Xueping Xie, Qi Zhang, Shu Zhang, Bofeng Zhu, Yunfeng Lin, Xiaoxiao Cai
Cells sense and respond to the biophysical properties of their surrounding environment by interacting with the extracellular matrix (ECM). Therefore, the optimization of these cell-matrix interactions is critical in tissue engineering. The vascular system is adapted to specific functions in diverse tissues and organs. Appropriate arterial-venous differentiation is vital for the establishment of functional vasculature in angiogenesis. Here, we have developed a polydimethylsiloxane (PDMS)-based substrate capable of simulating the physiologically relevant stiffness of both venous (7 kPa) and arterial (128 kPa) tissues...
July 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28728878/a-mass-spectrometry-based-proteomic-analysis-of-homer2-interacting-proteins-in-the-mouse-brain
#4
Scott P Goulding, Karen K Szumlinski, Candice Contet, Michael J MacCoss, Christine C Wu
In the brain, the Homer protein family modulates excitatory signal transduction and receptor plasticity through interactions with other proteins in dendritic spines. Homer proteins are implicated in a variety of psychiatric disorders such as schizophrenia and addiction. Since Homers serve as scaffolding proteins, identifying their interaction partners is an important first step in understanding their biological function and could help design novel therapeutic strategies. The present study set out to document Homer2-interacting proteins in the mouse brain using a co-immunoprecipitation-based mass spectrometry approach...
July 17, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/28724790/med12-regulates-a-transcriptional-network-of-calcium-handling-genes-in-the-heart
#5
Kedryn K Baskin, Catherine A Makarewich, Susan M DeLeon, Wenduo Ye, Beibei Chen, Nadine Beetz, Heinrich Schrewe, Rhonda Bassel-Duby, Eric N Olson
The Mediator complex regulates gene transcription by linking basal transcriptional machinery with DNA-bound transcription factors. The activity of the Mediator complex is mainly controlled by a kinase submodule that is composed of 4 proteins, including MED12. Although ubiquitously expressed, Mediator subunits can differentially regulate gene expression in a tissue-specific manner. Here, we report that MED12 is required for normal cardiac function, such that mice with conditional cardiac-specific deletion of MED12 display progressive dilated cardiomyopathy...
July 20, 2017: JCI Insight
https://www.readbyqxmd.com/read/28718627/structural-basis-for-interaction-of-the-tandem-zinc-finger-domains-of-human-muscleblind-with-cognate-rna-from-human-cardiac-troponin-t
#6
Sangho Park, Priti Deka Phukan, Markus Zeeb, Maria A Martinez-Yamout, Helen Jane Dyson, Peter E Wright
The human muscleblind-like proteins (MBNL) regulate tissue-specific splicing by targeting cardiac troponin T and other pre-mRNAs; aberrant targeting of CUG and CCUG repeat expansions frequently accompanies the neuromuscular disease myotonic dystrophy. We show, using biolayer interferometry (Octet), and NMR spectroscopy, that the zinc finger domains of MBNL isoform 1 (MBNL1) are necessary and sufficient for binding CGCU sequences within the pre-mRNA of human cardiac troponin T (hcTnT). Protein constructs containing zinc fingers 1 and 2 (zf12) and zinc fingers 3 and 4 (zf34) of MBNL1 each fold into a compact globular tandem zinc finger structure that participates in RNA binding...
July 18, 2017: Biochemistry
https://www.readbyqxmd.com/read/28715970/scaffold-in-scaffold-potential-to-induce-growth-and-differentiation-of-cardiac-progenitor-cells
#7
Matteo Ciocci, Federico Mochi, Felicia Carotenuto, Emilia Di Giovanni, Paolo Prosposito, Roberto Francini, Fabio De Matteis, Igor Reshetov, Mauro Casalboni, Sonia Melino, Paolo Di Nardo
The design of reliable biocompatible and biodegradable scaffolds remains one of the most important challenges for tissue engineering. In fact, properly designed scaffolds must display an adequate and interconnected porosity to facilitate cell spreading and colonization of the inner layers, and must release physical signals concurring to modulate cell function to ultimately drive cell fate. In the present study, a combination of optimal mechanical and biochemical properties has been considered to design a one-component 3D multitextured hydrogel scaffold in order to favor cell-scaffold interactions...
July 17, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28714474/facultative-ctcf-sites-moderate-mammary-super-enhancer-activity-and-regulate-juxtaposed-gene-in-non-mammary-cells
#8
M Willi, K H Yoo, F Reinisch, T M Kuhns, H K Lee, C Wang, L Hennighausen
Precise spatiotemporal gene regulation is paramount for the establishment and maintenance of cell-specific programmes. Although there is evidence that chromatin neighbourhoods, formed by the zinc-finger protein CTCF, can sequester enhancers and their target genes, there is limited in vivo evidence for CTCF demarcating super-enhancers and preventing cross talk between distinct regulatory elements. Here, we address these questions in the Wap locus with its mammary-specific super-enhancer separated by CTCF sites from widely expressed genes...
July 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/28714327/elevated-levels-of-circulating-cell-free-dna-and-neutrophil-proteins-are-associated-with-neonatal-sepsis-and-necrotizing-enterocolitis-in-immature-mice-pigs-and-infants
#9
Duc Ninh Nguyen, Allan Stensballe, Jacqueline Cy Lai, Pingping Jiang, Anders Brunse, Yanqi Li, Jing Sun, Carina Mallard, Tom Skeath, Nicholas D Embleton, Janet E Berrington, Per T Sangild
Preterm infants are highly susceptible to late-onset sepsis (LOS) and necrotizing enterocolitis (NEC), but disease pathogenesis and specific diagnostic markers are lacking. Circulating cell-free DNA (cfDNA) and immune cell-derived proteins are involved in multiple immune diseases in adults but have not been investigated in preterm neonates. We explored the relation of circulating neutrophil-associated proteins and cfDNA to LOS and/or NEC. Using a clinically relevant preterm pig model of spontaneous LOS and NEC development, we investigated neutrophil-associated proteins and cfDNA in plasma, together with cytokines in gut tissues...
January 1, 2017: Innate Immunity
https://www.readbyqxmd.com/read/28713917/stim1-silencing-inhibits-the-migration-and-invasion-of-a549-cells
#10
Yadong Wang, Haiyu Wang, Teng Pan, Li Li, Jiangmin Li, Haiyan Yang
The present study aimed to explore the effects of stromal interaction molecule 1 (STIM1) knockdown on the migration, invasion and metastasis of A549 cells in vitro and in vivo. Western blotting and immunohistochemistry were used to detect protein expression levels. Wound healing and Transwell invasion assays were used to assess the migratory and invasive abilities of A549 cells transfected with STIM1‑specific short hairpin (sh)RNA (shSTIM1). In addition, a tail vein metastatic assay was performed. The results demonstrated that the frequency of STIM1 high‑expression was significantly increased in metastatic lung cancer tissues (72...
September 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28713562/epithelial-spindle-orientation-diversities-and-uncertainties-recent-developments-and-lingering-questions
#11
REVIEW
Lindsey Seldin, Ian Macara
Mitotic spindle orientation is a conserved, dynamic, and highly complex process that plays a key role in dictating the cleavage plane, fate, and positioning of cells within a tissue, therefore laying the blueprint for tissue structure and function. While the spindle-positioning pathway has been extensively studied in lower-model organisms, research over the past several years has highlighted its relevance to mammalian epithelial tissues. Although we continue to gain critical insights into the mechanisms underlying spindle positioning, many uncertainties persist...
2017: F1000Research
https://www.readbyqxmd.com/read/28710687/disaggregation-and-reaggregation-of-zebrafish-retinal-cells-for-the-analysis-of-neuronal-layering
#12
Megan K Eldred, Leila Muresan, William A Harris
The reaggregation of dissociated cells to form organotypic structures provides an in vitro system for the analysis of the cellular interactions and molecular mechanisms involved in the formation of tissue architecture. The retina, an outgrowth of the forebrain, is a precisely layered neural tissue, yet the mechanisms underlying layer formation are largely unexplored. Here we describe the protocol to dissociate, re-aggregate, and culture zebrafish retinal cells from a transgenic, Spectrum of Fates, line where all main cell types are labelled with a combination of fluorescent proteins driven by fate-specific promoters...
July 15, 2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28710114/circadian-rhythm-disruption-impairs-tissue-homeostasis-and-exacerbates-chronic-inflammation-in-the-intestine
#13
René Pagel, Florian Bär, Torsten Schröder, Annika Sünderhauf, Axel Künstner, Saleh M Ibrahim, Stella E Autenrieth, Kathrin Kalies, Peter König, Anthony H Tsang, Dominik Bettenworth, Senad Divanovic, Hendrik Lehnert, Klaus Fellermann, Henrik Oster, Stefanie Derer, Christian Sina
Endogenous circadian clocks regulate 24 h rhythms of physiology and behavior. Circadian rhythm disruption (CRD) is suggested as a risk factor for inflammatory bowel disease. However, the underlying molecular mechanisms remain unknown. Intestinal biopsies from Per1/2 mutant and wild-type (WT) mice were investigated by electron microscopy, immunohistochemistry, and bromodeoxyuridine pulse-chase experiments. TNF-α was injected intraperitoneally, with or without necrostatin-1, into Per1/2 mice or rhythmic and externally desynchronized WT mice to study intestinal epithelial cell death...
July 14, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/28707887/full-characterization-of-localization-diversity-in-human-protein-interactome
#14
Lixin Cheng, Kaili Fan, Yan Huang, Dong Wang, Kwong-Sak Leung
Spatial-temporal regulation amongst proteins forms dynamic networks in cells. Co-existence in common cell compartments can improve biological reliability of the protein-protein interactions. However, this is usually overlooked by most proteomic studies and leads to unrealistic discoveries. In this paper, we systematically characterize the interaction localization diversity in human protein interactome using localization coefficient, a novel metric proposed for assessing how diversely the interactions localize among cell compartments...
July 14, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28701926/glutamate-deregulation-in-ketamine-induced-psychosis-a-potential-role-of-psd95-nmda-receptor-and-pmca-interaction
#15
Malwina Lisek, Bozena Ferenc, Maciej Studzian, Lukasz Pulaski, Feng Guo, Ludmila Zylinska, Tomasz Boczek
Ketamine causes psychotic episodes and is often used as pharmacological model of psychotic-like behavior in animals. There is increasing evidence that molecular mechanism of its action is more complicated than just N-methyl-D-aspartic acid (NMDA) receptor antagonism and involves interaction with the components of calcium homeostatic machinery, in particular plasma membrane calcium pump (PMCA). Therefore, in this study we aimed to characterize brain region-specific effects of ketamine on PMCA activity, interaction with NMDA receptor through postsynaptic density protein 95 (PSD95) scaffolding proteins and glutamate release from nerve endings...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28701259/markers-of-protein-synthesis-are-increased-in-fetal-membranes-and-myometrium-after-human-labour-and-delivery
#16
Stella Liong, Martha Lappas
Preterm birth remains one of the leading causes of neonatal death. Inflammation and maternal infection are two of the leading aetiological factors for preterm birth. Labour is associated with increased production of proinflammatory cytokines, chemokines and prolabour mediators in human gestational tissues. In non-gestational tissues, synthesis of proinflammatory and prolabour mediators is regulated by components of the protein synthesis machinery. Therefore, in the present study we investigated the effect of human labour on the expression of three protein synthesis markers, namely eukaryotic elongation factor 2 kinase (EEF2K), mitogen-activated protein kinase interacting protein kinase 1 (MKNK1) and eukaryotic translation initiation factor 4E (EIF4E), and their role in regulating inflammation in human gestational tissues...
July 13, 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28700469/microarray-analysis-of-copy-number-variations-and-gene-expression-profiles-in-prostate-cancer
#17
Yuping Han, Xuefei Jin, Hongyan Li, Kaichen Wang, Ji Gao, Lide Song, Yanting Lv
BACKGROUND: This study aimed to identify potential prostate cancer (PC)-related variations in gene expression profiles. METHODS: Microarray data from the GSE21032 dataset that contained the whole-transcript and exon-level expression profile (GSE21034) and Agilent 244K array-comparative genomic hybridization data (GSE21035) were downloaded from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) and copy-number variations (CNVs) were identified between PC and normal tissue samples...
July 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28699676/influence-of-multi-wavelength-laser-irradiation-of-enamel-and-dentin-surfaces-at-0-355-2-94-and-9-4%C3%A2-%C3%AE-m-on-surface-morphology-permeability-and-acid-resistance
#18
Nai-Yuan N Chang, Jamison M Jew, Jacob C Simon, Kenneth H Chen, Robert C Lee, William A Fried, Jinny Cho, Cynthia L Darling, Daniel Fried
OBJECTIVE: Ultraviolet (UV) and infrared (IR) lasers can be used to specifically target protein, water, and mineral, respectively, in dental hard tissues to produce varying changes in surface morphology, permeability, reflectivity, and acid resistance. The purpose of this study was to explore the influence of laser irradiation and topical fluoride application on the surface morphology, permeability, reflectivity, and acid resistance of enamel and dentin to shed light on the mechanism of interaction and develop more effective treatments...
July 12, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28698308/the-role-of-attenuated-redox-and-heat-shock-protein-responses-in-the-age-related-decline-in-skeletal-muscle-mass-and-function
#19
REVIEW
Anne McArdle, Malcolm J Jackson
The loss of muscle mass and weakness that accompanies ageing is a major contributor to physical frailty and loss of independence in older people. A failure of muscle to adapt to physiological stresses such as exercise is seen with ageing and disruption of redox regulated processes and stress responses are recognized to play important roles in theses deficits. The role of redox regulation in control of specific stress responses, including the generation of heat shock proteins (HSPs) by muscle appears to be particularly important and affected by ageing...
July 15, 2017: Essays in Biochemistry
https://www.readbyqxmd.com/read/28698227/comparative-transcriptomics-of-hepatic-differentiation-of-human-pluripotent-stem-cells-and-adult-human-liver-tissue
#20
Nidal Ghosheh, Barbara Küppers-Munther, Annika Asplund, Josefina Edsbagge, Benjamin Ulfenborg, Tommy B Andersson, Petter Björquist, Christian X Andersson, Helena Carén, Stina Simonsson, Peter Sartipy, Jane Synnergren
Hepatocyte derived from human pluripotent stem cells (hPSC-HEP) have the potential to replace presently used hepatocyte sources applied in liver disease treatment and models of drug discovery and development. Established hepatocyte differentiation protocols are effective and generate hepatocytes, which recapitulate some key features of their in vivo counterparts. However, generating mature hPSC-HEP remains a challenge. In this study, we applied transcriptomics to investigate the progress of in vitro hepatic differentiation of hPSCs at the developmental stages, definitive endoderm (DE), hepatoblasts, early hPSC-HEP, and mature hPSC-HEP, to identify functional targets that enhance efficient hepatocyte differentiation...
July 10, 2017: Physiological Genomics
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