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Tissue specific protein interactions

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https://www.readbyqxmd.com/read/27900891/chondrogenesis-of-human-adipose-derived-stem-cells-for-future-microtia-repair-using-co-culture-technique
#1
Bee See Goh, Siti Nurhadis Che Omar, Muhammad Azhan Ubaidah, Lokman Saim, Shamsul Sulaiman, Kien Hui Chua
CONCLUSION: In conclusion, these result showed HADSCs could differentiate into chondrocytes-like cells, dependent on signaling induced by TGF-β3 and chondrocytes. This is a promising result and showed that HADSCs is a potential source for future microtia repair. The technique of co-culture is a positive way forward to assist the microtia tissue. OBJECTIVE: Reconstructive surgery for the repair of microtia still remains the greatest challenge among the surgeons...
November 30, 2016: Acta Oto-laryngologica
https://www.readbyqxmd.com/read/27899616/the-tissuenet-v-2-database-a-quantitative-view-of-protein-protein-interactions-across-human-tissues
#2
Omer Basha, Ruth Barshir, Moran Sharon, Eugene Lerman, Binyamin F Kirson, Idan Hekselman, Esti Yeger-Lotem
Knowledge of the molecular interactions of human proteins within tissues is important for identifying their tissue-specific roles and for shedding light on tissue phenotypes. However, many protein-protein interactions (PPIs) have no tissue-contexts. The TissueNet database bridges this gap by associating experimentally-identified PPIs with human tissues that were shown to express both pair-mates. Users can select a protein and a tissue, and obtain a network view of the query protein and its tissue-associated PPIs...
November 29, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27899064/gdf5-mediated-enhancement-of-chondrocyte-phenotype-is-inhibited-by-heparin-implication-for-the-use-of-heparin-in-the-clinic-and-in-tissue-engineering-applications
#3
Bethanie Imogen Ayerst, Raymond A A Smith, Victor Nurcombe, Anthony J Day, Catherine L R Merry, Simon Cool
The highly sulfated glycosaminoglycan (GAG) heparin is widely used in the clinic as an anticoagulant, and researchers are now using it to enhance stem cell expansion/ differentiation protocols, as well as to improve the delivery of growth factors for tissue engineering strategies. Growth differentiation factor 5 (GDF5) belongs to the bone morphogenetic protein family of proteins and is vital for skeletal formation, however, its interaction with heparin and heparan sulfate (HS) has not been studied. We identify GDF5 as a novel heparin/ HS-binding protein, and show that HS proteoglycans are vital in localizing GDF5 to the cell surface...
November 29, 2016: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/27898393/a-noncanonical-auxin-sensing-mechanism-is-required-for-organ-morphogenesis-in-arabidopsis
#4
Sara Simonini, Joyita Deb, Laila Moubayidin, Pauline Stephenson, Manoj Valluru, Alejandra Freire-Rios, Karim Sorefan, Dolf Weijers, Jiří Friml, Lars Østergaard
Tissue patterning in multicellular organisms is the output of precise spatio-temporal regulation of gene expression coupled with changes in hormone dynamics. In plants, the hormone auxin regulates growth and development at every stage of a plant's life cycle. Auxin signaling occurs through binding of the auxin molecule to a TIR1/AFB F-box ubiquitin ligase, allowing interaction with Aux/IAA transcriptional repressor proteins. These are subsequently ubiquitinated and degraded via the 26S proteasome, leading to derepression of auxin response factors (ARFs)...
October 15, 2016: Genes & Development
https://www.readbyqxmd.com/read/27893851/expression-and-regulation-of-piwil-proteins-and-piwi-interacting-rnas-in-rheumatoid-arthritis
#5
Lenka Pleštilová, Michel Neidhart, Giancarlo Russo, Mojca Frank-Bertoncelj, Caroline Ospelt, Adrian Ciurea, Christoph Kolling, Renate E Gay, Beat A Michel, Jiří Vencovský, Steffen Gay, Astrid Jüngel
OBJECTIVE: The PIWIL (P-element induced wimpy testis like protein) subfamily of argonaute proteins is essential for Piwi-interacting RNA (piRNA) biogenesis and their function to silence transposons during germ-line development. Here we explored their presence and regulation in rheumatoid arthritis (RA). METHODS: The expression of PIWIL genes in RA and osteoarthritis (OA) synovial tissues and synovial fibroblasts (SF) was analysed by Real-time PCR, immunofluorescence and Western blot...
2016: PloS One
https://www.readbyqxmd.com/read/27893441/engineering-tumor-cell-targeting-in-nanoscale-amyloidal-materials
#6
Ugutz Unzueta, Joaquin Seras-Franzoso, María Virtudes Céspedes, Paolo Saccardo, Francisco Cortés, Fabián Rueda, Elena Garcia-Fruitós, Neus Ferrer-Miralles, Ramon Mangues, Esther Vázquez, Antonio Villaverde
Bacterial inclusion bodies are non-toxic, mechanically stable and functional protein amyloids within the nanoscale size range that are able to naturally penetrate into mammalian cells, where they deliver the embedded protein in a functional form. The potential use of inclusion bodies in protein delivery or protein replacement therapies is strongly impaired by the absence of specificity in cell binding and penetration, thus preventing targeting. To address this issue, we have here explored whether the genetic fusion of two tumor-homing peptides, the CXCR4 ligands R9 and T22, to an inclusion body-forming green fluorescent protein (GFP), would keep the interaction potential and the functionality of the fused peptides and then confer CXCR4 specificity in cell binding and further uptake of the materials...
November 28, 2016: Nanotechnology
https://www.readbyqxmd.com/read/27885213/a-fret-biosensor-for-rock-based-on-a-consensus-substrate-sequence-identified-by-kiss-technology
#7
Chunjie Li, Ayako Imanishi, Naoki Komatsu, Kenta Terai, Mutsuki Amano, Kozo Kaibuchi, Michiyuki Matsuda
Genetically-encoded biosensors based on Förster/fluorescence resonance energy transfer (FRET) are versatile tools for studying the spatio-temporal regulation of signaling molecules within not only the cells but also tissues. Perhaps the hardest task in the development of a FRET biosensor for protein kinases is to identify the kinase-specific substrate peptide to be used in the FRET biosensor. To solve this problem, we took advantage of kinase-interacting substrate screening (KISS) technology, which deduces a consensus substrate sequence for the protein kinase of interest...
November 23, 2016: Cell Structure and Function
https://www.readbyqxmd.com/read/27883932/biological-response-of-human-suture-mesenchymal-cells-to-titania-nanotube-based-implants-for-advanced-craniosynostosis-therapy
#8
Manpreet Bariana, Prem Dwivedi, Sarbin Ranjitkar, John A Kaidonis, Dusan Losic, Peter J Anderson
Titania nanotubes (TNTs) engineered on titanium (Ti) surfaces (i.e. TNT/Ti) and loaded with specific drugs have been recognised as a promising solution for localised therapeutic delivery to address several medical problems not feasible with conventional drug administration. We propose the use of TNT/Ti protein-releasing implants to treat paediatric craniofacial abnormality in craniosynostosis caused by premature fusion of cranial sutures. In this study, we have analysed the biological response of human suture mesenchymal cells (SMCs), extracted from two different patients undergoing craniofacial reconstruction surgery, at the TNT/Ti implant surface...
November 17, 2016: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/27883130/nanobubbles-cavitation-shock-waves-and-traumatic-brain-injury
#9
Upendra Adhikari, Ardeshir Goliaei, Max L Berkowitz
Collapse of bubbles, microscopic or nanoscopic, due to their interaction with the impinging pressure wave produces a jet of particles moving in the direction of the wave. If there is a surface nearby, the high-speed jet particles hit it, and as a result damage to the surface is produced. This cavitation effect is well known and intensely studied in case of microscopic sized bubbles. It can be quite damaging to materials, including biological tissues, but it can also be beneficial when controlled, like in case of sonoporation of biological membranes for the purpose of drug delivery...
November 24, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27881660/the-norovirus-ns3-protein-is-a-dynamic-lipid-and-microtubule-associated-protein-involved-in-viral-rna-replication
#10
Ben Cotton, Jennifer L Hyde, Soroush T Sarvestani, Stanislav V Sosnovtsev, Kim Y Green, Peter A White, Jason M Mackenzie
: Norovirus (NoV) infections are a significant health burden to society yet the lack of reliable tissue culture systems has hampered the development of appropriate antiviral therapies. Here we show that the NoV NS3 protein, derived from murine NoV (MNV), is intimately associated with the MNV replication complex and the viral replication intermediate dsRNA. We have observed that when expressed individually MNV NS3 and NS3 encoded by human Norwalk virus (NV) induced the formation of distinct vesicle-like structures that did not co-localise with any particular protein markers to cellular organelles, but localised to cellular membranes, in particular those high in cholesterol content...
November 23, 2016: Journal of Virology
https://www.readbyqxmd.com/read/27878179/when-biomolecules-meet-graphene-from-molecular-level-interactions-to-material-design-and-applications
#11
REVIEW
Dapeng Li, Wensi Zhang, Xiaoqing Yu, Zhenping Wang, Zhiqiang Su, Gang Wei
Graphene-based materials have attracted increasing attention due to their atomically-thick two-dimensional structures, high conductivity, excellent mechanical properties, and large specific surface areas. The combination of biomolecules with graphene-based materials offers a promising method to fabricate novel graphene-biomolecule hybrid nanomaterials with unique functions in biology, medicine, nanotechnology, and materials science. In this review, we focus on a summarization of the recent studies in functionalizing graphene-based materials using different biomolecules, such as DNA, peptides, proteins, enzymes, carbohydrates, and viruses...
November 23, 2016: Nanoscale
https://www.readbyqxmd.com/read/27872094/mif-induced-stromal-pkc%C3%AE-il8-is-essential-in-human-acute-myeloid-leukemia
#12
Amina Abdul-Aziz, Manar Shafat, Tarang Mehta, Federica Di Palma, Matthew Lawes, Stuart A Rushworth, Kristian Bowles
Acute myeloid leukemia (AML) cells exhibit a high level of spontaneous apoptosis when cultured in vitro but have a prolonged survival time in vivo, indicating that tissue microenvironment plays a critical role in promoting AML cell survival. In vitro studies have shown that bone marrow-mesenchymal stromal cells (BM-MSC) protect AML blasts from spontaneous and chemotherapy-induced apoptosis. Here we report a novel interaction between AML blasts and BM-MSC which benefits AML proliferation and survival. We initially examined the cytokine profile in cultured human AML compared to AML cultured with BM-MSC and found that macrophage-migration inhibitory factor (MIF) was highly expressed by primary AML, and that interleukin-8 (IL-8) was increased in AML/BM-MSC co-cultures...
November 21, 2016: Cancer Research
https://www.readbyqxmd.com/read/27871939/cell-cell-communication-mediated-by-the-car-subgroup-of-immunoglobulin-cell-adhesion-molecules-in-health-and-disease
#13
REVIEW
Claudia Matthäus, Hanna Langhorst, Laura Schütz, René Jüttner, Fritz G Rathjen
The immunoglobulin superfamily represents a diverse set of cell-cell contact proteins and includes well-studied members such as NCAM1, DSCAM, L1 or the contactins which are strongly expressed in the nervous system. In this review we put our focus on the biological function of a less understood subgroup of Ig-like proteins composed of CAR (coxsackievirus and adenovirus receptor), CLMP (CAR-like membrane protein) and BT-IgSF (brain and testis specific immunoglobulin superfamily). The CAR-related proteins are type I transmembrane proteins containing an N-terminal variable (V-type) and a membrane proximal constant (C2-type) Ig domain in their extracellular region which are implicated in homotypic adhesion...
November 18, 2016: Molecular and Cellular Neurosciences
https://www.readbyqxmd.com/read/27871336/zfas1-a-long-noncoding-rna-associated-with-ribosomes-in-breast-cancer-cells
#14
Herah Hansji, Euphemia Y Leung, Bruce C Baguley, Graeme J Finlay, David Cameron-Smith, Vandre C Figueiredo, Marjan E Askarian-Amiri
BACKGROUND: Most of the eukaryotic genome is transcribed, yielding a complex network of transcripts including thousands of lncRNAs that generally lack protein coding potential. However, only a small percentage of these molecules has been functionally characterised, and discoveries of specific functions demonstrate layers of complexity. A large percentage of lncRNAs is located in the cytoplasm, associated with ribosomes but the function of the majority of these transcripts is unclear. The current study analyses putative mechanisms of action of the lncRNA species member ZFAS1 that was initially discovered by microarray analysis of murine tissues undergoing mammary gland development...
November 21, 2016: Biology Direct
https://www.readbyqxmd.com/read/27869151/the-lectin-like-protein-1-in-lactobacillus-rhamnosus-gr-1-mediates-tissue-specific-adherence-to-vaginal-epithelium-and-inhibits-urogenital-pathogens
#15
Mariya I Petrova, Elke Lievens, Tine L A Verhoeven, Jean M Macklaim, Gregory Gloor, Dominique Schols, Jos Vanderleyden, Gregor Reid, Sarah Lebeer
The probiotic Lactobacillus rhamnosus GR-1 has been documented to survive implantation onto the vaginal epithelium and interfere with urogenital pathogens. However, the molecular mechanisms involved are largely unknown. Here, we report for the first time the construction of dedicated knock-out mutants in L. rhamnosus GR-1 to enable the study of gene functions. In a search for genes responsible for the adherence capacity of L. rhamnosus GR-1, a genomic region encoding a protein with homology to lectin-like proteins was identified...
November 21, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27868160/severe-changes-in-colon-epithelium-in-the-mecp2-null-mouse-model-of-rett-syndrome
#16
Pamela Millar-Büchner, Amber R Philp, Noemí Gutierrez, Sandra Villanueva, Bredford Kerr, Carlos A Flores
BACKGROUND: Rett syndrome is best known due to its severe and devastating symptoms in the central nervous system. It is produced by mutations affecting the Mecp2 gene that codes for a transcription factor. Nevertheless, evidence for MECP2 activity has been reported for tissues other than those of the central nervous system. Patients affected by Rett presented with intestinal affections whose origin is still not known. We have observed that the Mecp2-null mice presented with episodes of diarrhea, and decided to study the intestinal phenotype in these mice...
December 2016: Molecular and Cellular Pediatrics
https://www.readbyqxmd.com/read/27864543/long-non-coding-rnas-a-novel-paradigm-for-toxicology
#17
REVIEW
Joseph L Dempsey, Julia Yue Cui
Long non-coding RNAs (lncRNAs) are over 200 nucleotides in length and are transcribed from the mammalian genome in a tissue-specific and developmentally regulated pattern. There is growing recognition that lncRNAs are novel biomarkers and/or key regulators of toxicological responses in humans and animal models. Lacking protein-coding capacity, the numerous types of lncRNAs possess a myriad of transcriptional regulatory functions that include cis and trans gene expression, transcription factor activity, chromatin remodeling, imprinting, and enhancer up-regulation...
November 17, 2016: Toxicological Sciences: An Official Journal of the Society of Toxicology
https://www.readbyqxmd.com/read/27864353/identification-of-clinically-predictive-metagenes-that-encode-components-of-a-network-coupling-cell-shape-to-transcription-by-image-omics
#18
Heba Z Sailem, Chris Bakal
The associations between clinical phenotypes (tumour grade, survival), and cell phenotypes, such as shape, signalling activity, and gene expression, are the basis for cancer pathology; but the mechanisms explaining these relationships are not always clear. The generation of large datasets containing information regarding cell phenotypes, and clinical data, provides an opportunity to describe these mechanisms. Here we develop an image-omics approach to integrate quantitative cell imaging data, gene expression, and protein-protein interaction data to systematically describe a 'shape-gene network' that couples specific aspects of breast cancer cell shape to signalling and transcriptional events...
November 18, 2016: Genome Research
https://www.readbyqxmd.com/read/27862637/weighing-biointeractions-between-fibrin-ogen-and-clot-binding-peptides-using-microcantilever-sensors
#19
Anna Puiggalí-Jou, Luis J Del Valle, Carlos Alemán, Maria M Pérez-Madrigal
Peptides homing tumor vasculature are considered promising molecular imaging agents for cancer detection at an early stage. In addition to their high binding affinity, improved tissue penetrating ability, and low immunogenicity, they can deliver targeted anticancer drugs, thus expanding therapeutic treatments. Among those, CREKA, a linear peptide that specifically binds to clotted-plasma proteins in tumor vessels, has been recently employed to design bioactive systems able to target different cancer types. Within this context, this paper explores the biorecognition event between CR(NMe)EKA, an engineered CREKA-analog bearing a noncoded amino acid (N-methyl-Glu) that is responsible for its enhanced activity, and clotted-plasma proteins (fibrin and fibrinogen) by nanomechanical detection...
November 14, 2016: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/27858498/sun1-splice-variants-sun1_888-sun1_785-and-predominant-sun1_916-variably-function-in-directional-cell-migration
#20
Yu Nishioka, Hiromasa Imaizumi, Junko Imada, Jun Katahira, Nariaki Matsuura, Miki Hieda
The LINC complex is a multifunctional protein complex that is involved in various processes at the nuclear envelope, such as nuclear migration, mechanotransduction and chromatin tethering in the meiotic phase. However, it remains unknown how these functions are regulated in different cell contexts. An inner nuclear membrane component of the LINC complex, SUN1, is ubiquitously expressed. The human SUN1 gene produces over 10 variants by alternative splicing. Although functions of SUN1 are relatively well characterized, functional differences among SUN1 splice variants are poorly characterized...
November 18, 2016: Nucleus
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