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https://www.readbyqxmd.com/read/29351256/immobilization-of-staphylococcus-aureus-sortase-a-on-chitosan-particles-and-its-applications-in-peptide-to-peptide-ligation-and-peptide-cyclization
#1
Min Yang, Haofei Hong, Shaozhong Liu, Xinrui Zhao, Zhimeng Wu
Chitosan macro-particles prepared by the neutralization method were applied to Sortase A (SrtA) immobilization using glutaraldehyde as a crosslinking agent. The particles were characterized by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Response surface methodology (RSM) was employed to optimize the immobilization process. An average specific activity of 3142 U (mg protein)-1 was obtained under optimized immobilization conditions (chitosan concentration 3%, SrtA concentration 0...
January 19, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29351206/antibody-biomimetic-material-made-of-pyrrole-for-ca-15-3-and-its-application-as-sensing-material-in-ion-selective-electrodes-for-potentiometric-detection
#2
Alexandra R T Santos, Felismina T C Moreira, Luísa A Helguero, M Goreti F Sales
This work reports a very simple approach for creating a synthetic antibody against any protein of interest and its application in potentiometric transduction. The selected protein was Breast Cancer Antigen (CA 15-3), which is implicated in breast cancer disease and used to follow-up breast cancer patients during treatment. The new material with antibody-like properties was obtained by molecular-imprinting technology, prepared by electropolymerizing pyrrol (Py, 5.0 × 10-3 mol/L) around Breast Cancer Antigen (CA 15-3) (100 U/mL) on a fluorine doped tin oxide (FTO) conductive glass support...
January 19, 2018: Biosensors
https://www.readbyqxmd.com/read/29349625/enhancement-of-the-thermal-and-alkaline-ph-stability-of-escherichia-coli-lysine-decarboxylase-for-efficient-cadaverine-production
#3
Fengyu Kou, Jing Zhao, Jiao Liu, Cunmin Sun, Yanmei Guo, Zijian Tan, Feng Cheng, Zhimin Li, Ping Zheng, Jibin Sun
OBJECTIVE: To enhance the thermal and alkaline pH stability of the lysine decarboxylase from Escherichia coli (CadA) by engineering the decameric interface and explore its potential for industrial applications. RESULTS: The mutant T88S was designed for improved structural stability by computational analysis. The optimal pH and temperature of T88S were 7.0 and 55 °C (5.5 and 50 °C for wild-type). T88S showed higher thermostability with a 2.9-fold increase in the half-life at 70 °C (from 11 to 32 min) and increased melting temperature (from 76 to 78 °C)...
January 18, 2018: Biotechnology Letters
https://www.readbyqxmd.com/read/29349547/heterologous-expression-of-two-aspergillus-niger-feruloyl-esterases-in-trichoderma-reesei-for-the-production-of-ferulic-acid-from-wheat-bran
#4
Liangkun Long, Haoyuan Zhao, Dafan Ding, Meijuan Xu, Shaojun Ding
Feruloyl esterase (FAE)-encoding genes AnfaeA and AnfaeB were isolated from Aspergillus niger 0913. For overexpression of the two genes in Trichoderma reesei, constitutive and inductive expression plasmids were constructed based on parental plasmid pAg1-H3. The constructed plasmids contained AnfaeA or AnfaeB gene under the control of glyceraldehyde-3-phosphate dehydrogenase A gene (gpdA) promoter (from A. nidulans) or cellobiohydrolases I (cbh I) gene promoter (from T. reesei), and cbh I terminator from T. reesei...
January 18, 2018: Bioprocess and Biosystems Engineering
https://www.readbyqxmd.com/read/29349042/an-optimized-targeted-next-generation-sequencing-approach-for-sensitive-detection-of-single-nucleotide-variants
#5
S Stasik, C Schuster, C Ortlepp, U Platzbecker, M Bornhäuser, J Schetelig, G Ehninger, G Folprecht, C Thiede
Monitoring of minimal residual disease (MRD) has become an important clinical aspect for early relapse detection during follow-up care after cancer treatment. Still, the sensitive detection of single base pair point mutations via Next-Generation Sequencing (NGS) is hampered mainly due to high substitution error rates. We evaluated the use of NGS for the detection of low-level variants on an Ion Torrent PGM system. As a model case we used the c.1849G > T (p.Val617Phe) mutation of the JAK2-gene. Several reaction parameters (e...
May 2018: Biomolecular Detection and Quantification
https://www.readbyqxmd.com/read/29348700/open-air-sprays-for-capturing-and-controlling-airborne-float-coal-dust-on-longwall-faces
#6
T W Beck, C E Seaman, M R Shahan, S E Mischler
Float dust deposits in coal mine return airways pose a risk in the event of a methane ignition. Controlling airborne dust prior to deposition in the return would make current rock dusting practices more effective and reduce the risk of coal-dust-fueled explosions. The goal of this U.S. National Institute for Occupational Safety and Health study is to determine the potential of open-air water sprays to reduce concentrations of airborne float coal dust, smaller than 75 µm in diameter, in longwall face airstreams...
January 2018: Mining Engineering
https://www.readbyqxmd.com/read/29348699/industrial-internet-of-things-iiot-applications-in-underground-coal-mines
#7
C Zhou, N Damiano, B Whisner, M Reyes
The Industrial Internet of Things (IIoT), a concept that combines sensor networks and control systems, has been employed in several industries to improve productivity and safety. U.S. National Institute for Occupational Safety and Health (NIOSH) researchers are investigating IIoT applications to identify the challenges of and potential solutions for transferring IIoT from other industries to the mining industry. Specifically, NIOSH has reviewed existing sensors and communications network systems used in U.S...
December 2017: Mining Engineering
https://www.readbyqxmd.com/read/29347529/percolation-thresholds-in-hyperbolic-lattices
#8
Stephan Mertens, Cristopher Moore
We use invasion percolation to compute numerical values for bond and site percolation thresholds p_{c} (existence of an infinite cluster) and p_{u} (uniqueness of the infinite cluster) of tesselations {P,Q} of the hyperbolic plane, where Q faces meet at each vertex and each face is a P-gon. Our values are accurate to six or seven decimal places, allowing us to explore their functional dependency on P and Q and to numerically compute critical exponents. We also prove rigorous upper and lower bounds for p_{c} and p_{u} that can be used to find the scaling of both thresholds as a function of P and Q...
October 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347310/quantum-heat-engine-with-coupled-superconducting-resonators
#9
Ali Ü C Hardal, Nur Aslan, C M Wilson, Özgür E Müstecaplıoğlu
We propose a quantum heat engine composed of two superconducting transmission line resonators interacting with each other via an optomechanical-like coupling. One resonator is periodically excited by a thermal pump. The incoherently driven resonator induces coherent oscillations in the other one due to the coupling. A limit cycle, indicating finite power output, emerges in the thermodynamical phase space. The system implements an all-electrical analog of a photonic piston. Instead of mechanical motion, the power output is obtained as a coherent electrical charging in our case...
December 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347294/-13-c-nmr-study-of-the-director-distribution-adopted-by-the-modulated-nematic-phases-formed-by-liquid-crystal-dimers-with-odd-numbers-of-atoms-in-their-spacers
#10
J W Emsley, M Lelli, G R Luckhurst, H Zimmermann
The orientational order of the molecules in the bent mesogen CB6OCB has been studied throughout the range of temperature stability of both the N_{U} and N_{TB} liquid-crystal phases by ^{13}C NMR spectroscopy. These spectra provide local order parameters for the para axes of both of the nonequivalent cyanobiphenyl groups and show how they change on entering the twist-bend nematic phase. A key feature of the order parameters is a weak, but clear maximum in the temperature variation of the order parameter prior to the N_{TB} phase...
December 2017: Physical Review. E
https://www.readbyqxmd.com/read/29347004/self-propulsion-against-a-moving-membrane-enhanced-accumulation-and-drag-force
#11
U Marini Bettolo Marconi, A Sarracino, C Maggi, A Puglisi
Self-propulsion (SP) is a main feature of active particles (AP), such as bacteria or biological micromotors, distinguishing them from passive colloids. A renowned consequence of SP is accumulation at static interfaces, even in the absence of hydrodynamic interactions. Here we address the role of SP in the interaction between AP and a moving semipermeable membrane. In particular, we implement a model of noninteracting AP in a channel crossed by a partially penetrable wall, moving at a constant velocity c. With respect to both the cases of passive colloids with c>0 and AP with c=0, the AP with finite c show enhancement of accumulation in front of the obstacle and experience a largely increased drag force...
September 2017: Physical Review. E
https://www.readbyqxmd.com/read/29346522/serious-fall-injury-history-and-adverse-health-outcomes-after-initiating-hemodialysis-among-older-u-s-adults
#12
C Barrett Bowling, Rasheeda Hall, Anjali Khakharia, Harold A Franch, Laura C Plantinga
Background: Although older adults with pre-dialysis chronic kidney disease (CKD) are at higher risk for falls, the prognostic significance of a serious fall injury prior to dialysis initiation has not been well described in the end-stage renal disease population. Methods: We examined the association between a serious fall injury in the year prior to starting hemodialysis and adverse health outcomes in the year following dialysis initiation using a retrospective cohort study of U...
January 16, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29346342/attention-deficit-hyperactivity-disorder-medication-prescription-claims-among-privately-insured-women-aged-15-44-years-united-states-2003-2015
#13
Kayla N Anderson, Elizabeth C Ailes, Melissa Danielson, Jennifer N Lind, Sherry L Farr, Cheryl S Broussard, Sarah C Tinker
Attention-deficit/hyperactivity disorder (ADHD) is a neurodevelopmental disorder that affects individuals across the lifespan. ADHD medication use among pregnant women is increasing (1), but consensus about the safety of ADHD medication use during pregnancy is lacking. Given that nearly half of U.S. pregnancies are unintended (2), and early pregnancy is a critical period for fetal development, examining trends in ADHD medication prescriptions among reproductive-aged women is important to quantify the population at risk for potential exposure...
January 19, 2018: MMWR. Morbidity and Mortality Weekly Report
https://www.readbyqxmd.com/read/29346097/change-detection-in-heterogenous-remote-sensing-images-via-homogeneous-pixel-transformation
#14
Zhunga Liu, Gang Li, Gregoire Mercier, You He, Quan Pan
The change detection in heterogeneous remote sensing images remains an important and open problem for damage assessment. We propose a new change detection method for heterogeneous images (i.e., SAR and optical images) based on homogeneous pixel transformation (HPT). HPT transfers one image from its original feature space (e.g., gray space) to another space (e.g., spectral space) in pixel-level to make the pre-event and post-event images represented in a common space for the convenience of change detection. HPT consists of two operations, i...
April 2018: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
https://www.readbyqxmd.com/read/29345314/the-prevalence-and-distribution-of-sheep-scab-in-wales-a-farmer-questionnaire-survey
#15
C-A Chivers, H Rose Vineer, R Wall
Outbreaks of ovine psoroptic mange in the U.K. have increased 100-fold since its deregulation in 1992, with the highest prevalence in Wales, a region of high sheep density. A cross-sectional, retrospective, questionnaire-based survey of 7500 members of the association of Welsh lamb and beef farmers [Welsh Lamb and Beef Producers Ltd (WLBP)] was used to investigate the prevalence and distribution of sheep scab in this region in 2015. The survey was completed by 14.0% (n = 972) of potential respondents. Scab outbreaks were reported on 15...
January 18, 2018: Medical and Veterinary Entomology
https://www.readbyqxmd.com/read/29345190/magnetoelectric-nanoparticles-for-delivery-of-antitumor-peptides-into-glioblastoma-cells-by-magnetic-fields
#16
Tiffanie S Stewart, Abhignyan Nagesetti, Rakesh Guduru, Ping Liang, Emmanuel Stimphil, Ali Hadjikhani, Luis Salgueiro, Jeffrey Horstmyer, Renzhi Cai, Andrew Schally, Sakhrat Khizroev
AIM: We studied externally controlled anticancer effects of binding tumor growth inhibiting synthetic peptides to magnetoelectric nanoparticles (MENs) on treatment of glioblastomas. METHODS: Hydrothermally synthesized 30-nm MENs had the core-shell composition of CoFe2O4@BaTiO3. Molecules of growth hormone-releasing hormone antagonist of the MIA class (MIA690) were chemically bound to MENs. In vitro experiments utilized human glioblastoma cells (U-87MG) and human brain microvascular endothelial cells...
January 18, 2018: Nanomedicine
https://www.readbyqxmd.com/read/29344087/determination-of-the-native-features-of-the-exoglucanase-cel48s-from-clostridium-thermocellum
#17
Ya-Jun Liu, Shiyue Liu, Sheng Dong, Renmin Li, Yingang Feng, Qiu Cui
Background: Clostridium thermocellum is considered one of the most efficient natural cellulose degraders because of its cellulosomal system. As the major exoglucanase of cellulosome in C. thermocellum, Cel48S plays key roles and influences the activity and features of cellulosome to a great extent. Thus, it is of great importance to reveal the enzymatic features of Cel48S. However, Cel48S has not been well performed due to difficulties in purifying either recombinant or native Cel48S proteins...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29343961/receiver-operating-characteristic-curve-analysis-of-the-child-behavior-checklist-and-teacher-s-report-form-for-assessing-autism-spectrum-disorder-in-preschool-aged-children
#18
Tiago S Bara, Antonio C Farias, Erico Pg Felden, Mara L Cordeiro
Background: Autism spectrum disorder (ASD) is a neurodevelopmental disorder characterized by social, behavioral, and communication impairments with an estimated prevalence of 1 in 68 school-aged children. There is a need for objective and easily applicable instruments for early identification of autistic children to enable initiation of early interventions during a very sensitive period of brain development and, consequently, optimize prognosis. Here, we tested the utility of the Child Behavior Checklist (CBCL) and the Caregiver-Teacher's Report Form (C-TRF) scales for assessing ASD in Brazil, where ASD screening research is emergent...
2018: Neuropsychiatric Disease and Treatment
https://www.readbyqxmd.com/read/29343834/newly-discovered-late-triassic-baqing-eclogite-in-central-tibet-indicates-an-anticlockwise-west-east-qiangtang-collision
#19
Yu-Xiu Zhang, Xin Jin, Kai-Jun Zhang, Wei-Dong Sun, Jian-Ming Liu, Xiao-Yao Zhou, Li-Long Yan
The Triassic eclogite-bearing central Qiangtang metamorphic belt (CQMB) in the northern Tibetan Plateau has been debated whether it is a metamorphic core complex underthrust from the Jinsha Paleo-Tethys or an in-situ Shuanghu suture. The CQMB is thus a key issue to elucidate the crustal architecture of the northern Tibetan Plateau, the tectonics of the eastern Tethys, and the petrogenesis of Cenozoic high-K magmatism. We here report the newly discovered Baqing eclogite along the eastern extension of the CQMB near the Baqing town, central Tibet...
January 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29343686/human-brain-patterns-underlying-vigilant-attention-impact-of-sleep-debt-circadian-phase-and-attentional-engagement
#20
M Maire, C F Reichert, V Gabel, A U Viola, C Phillips, C Berthomier, S Borgwardt, C Cajochen, C Schmidt
Sleepiness and cognitive function vary over the 24-h day due to circadian and sleep-wake-dependent mechanisms. However, the underlying cerebral hallmarks associated with these variations remain to be fully established. Using functional magnetic resonance imaging (fMRI), we investigated brain responses associated with circadian and homeostatic sleep-wake-driven dynamics of subjective sleepiness throughout day and night. Healthy volunteers regularly performed a psychomotor vigilance task (PVT) in the MR-scanner during a 40-h sleep deprivation (high sleep pressure) and a 40-h multiple nap protocol (low sleep pressure)...
January 17, 2018: Scientific Reports
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