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https://www.readbyqxmd.com/read/28636636/sensiscreen%C3%A2-kras-exon-2-sensitive-simplex-and-multiplex-real-time-pcr-based-assays-for-detection-of-kras-exon-2-mutations
#1
Alice Riva, Michael BØrgesen, Mariann Guldmann-Christensen, Majbritt Hauge Kyneb, Kirsten Voogd, Christina Andersen, Samantha Epistolio, Elisabetta Merlo, Tine Yding Wolff, Stephen Hamilton-Dutoit, Jan Lorenzen, Ulf Bech Christensen, Milo Frattini
Activating mutations in codon 12 and codon 13 of the KRAS (Kirsten rat sarcoma viral oncogene homolog) gene are implicated in the development of several human cancer types and influence their clinical evaluation, treatment and prognosis. Numerous different methods for KRAS genotyping are currently available displaying a wide range of sensitivities, time to answer and requirements for laboratory equipment and user skills. Here we present SensiScreen® KRAS exon 2 simplex and multiplex CE IVD assays, that use a novel real-time PCR-based method for KRAS mutation detection based on PentaBase's proprietary DNA analogue technology and designed to work on standard real-time PCR instruments...
2017: PloS One
https://www.readbyqxmd.com/read/28624722/age-related-differences-in-the-response-of-the-l5-s1-intervertebral-disc-to-spinal-traction
#2
Ulrike H Mitchell, Paul F Beattie, Jennifer Bowden, Robert Larson, Hanuan Wang
BACKGROUND: Lumbar traction is a common treatment for low back pain; however its mechanisms of action are poorly understood. It has been hypothesized that a key effect of lumbar traction is its capacity to influence fluid movement within the intervertebral disc (IVD). OBJECTIVES: To determine differences in the apparent diffusion coefficient (ADC) obtained with lumbar diffusion-weighted imaging (DWI) of the L5-S1 IVD before, and during, the application of lumbar traction...
June 9, 2017: Musculoskeletal Science & Practice
https://www.readbyqxmd.com/read/28622896/biomechanical-modeling-of-spine-flexibility-and-its-relationship-to-spinal-range-of-motion-and-idiopathic-scoliosis
#3
Tishya A L Wren, Skorn Ponrartana, Ervin Poorghasamians, Sarah Moreau, Patricia C Aggabao, Tracy L Zaslow, Bianca R Edison, Vicente Gilsanz
STUDY DESIGN: Cross-sectional. OBJECTIVE: To examine the relationships between spine morphology, spine flexibility, and idiopathic scoliosis. BACKGROUND: Girls have a higher incidence of clinically significant scoliosis than boys, along with smaller vertebrae and greater flexibility. Based on biomechanical modeling, we hypothesized that smaller vertebral width relative to intervertebral disc (IVD) height would be associated with both greater lateral flexibility of the spine and with idiopathic scoliosis...
July 2017: Spine Deformity
https://www.readbyqxmd.com/read/28622016/endoplasmic-reticulum-stress-is-involved-in-nucleus-pulposus-degeneration-and-attenuates-low-ph-induced-apoptosis-of-rat-nucleus-pulposus-cells
#4
Zhi-Yang Xie, Lu Chen, Feng Wang, Lei Liu, Cong Zhang, Kun Wang, Feng Cai, Arjun Sinkemanni, Xin Hong, Xiao-Tao Wu
The microenvironment of degenerative intervertebral disk (IVD) is characteristic of a high concentration of lactic acid and low pH levels, whereas the underlying mechanism has not been clearly defined. Endoplasmic reticulum (ER) is the hub of interactions between environmental signals and cellular biological functions, the malfunction of which is closely involved in the pathogenesis of multiple disorders, including IVD degeneration (IVDD). This research mainly aims at exploring what role ER stress plays in the natural process of IVDD and pH-induced apoptosis of rat nucleus pulposus (NP) cells (NPCs)...
June 16, 2017: DNA and Cell Biology
https://www.readbyqxmd.com/read/28617524/high-prevalence-of-methicillin-resistant-coagulase-negative-staphylococci-isolated-from-a-university-environment-in-thailand
#5
Rathanin Seng, Udomluk Leungtongkam, Rapee Thummeepak, Wassana Chatdumrong, Sutthirat Sitthisak
The present study was conducted to isolate and characterize the molecular epidemiology of the methicillin-resistant staphylococci in the general university environment, where all five locations; the library, restrooms, canteens, computer rooms and outdoor surfaces, are in common use by a large population of students. We used Mannitol Salt Agar (MSA) supplemented with 4 μg/ml of oxacillin to screen the methicillin-resistant staphylococci. The species level was identified by PCR of rdr (Staphylococcus epidermidis), groESL (Staphylococcus haemolyticus) and nuc (Staphylococcus aureus and Staphylococcus warneri) genes and DNA sequencing of tuf and dnaJ genes...
June 2017: International Microbiology: the Official Journal of the Spanish Society for Microbiology
https://www.readbyqxmd.com/read/28612187/enhancement-of-energy-production-of-the-intervertebral-disc-by-the-implantation-of-polyurethane-mass-transfer-devices
#6
Yu-Fu Wang, Howard B Levene, Weiyong Gu, C -Y Charles Huang
Insufficient nutrient supply has been suggested to be one of the etiologies for intervertebral disc (IVD) degeneration. We are investigating nutrient transport into the IVD as a potential treatment strategy for disc degeneration. Most cellular activities in the IVD (e.g., cell proliferation and extracellular matrix production) are mainly driven by adenosine-5'-triphosphate (ATP) which is the main energy currency. The objective of this study was to investigate the effect of increased mass transfer on ATP production in the IVD by the implantation of polyurethane (PU) mass transfer devices...
June 13, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28606605/cervical-and-thoracic-intervertebral-disc-hydration-increases-with-recumbency-a-study-in-101-healthy-volunteers
#7
Daniel L Belavy, Matthew Quittner, Yuan Ling, David Connell, Timo Rantalainen
BACKGROUND CONTEXT: Variation in water content and size of the lumbar intervertebral discs (IVDs) is known to occur due to recumbency and has been associated with lumbar IVD herniation risk through the impact of IVD hydration on tissue mechanical properties. It is not clear if similar changes in the cervical or thoracic IVDs occur with recumbency. PURPOSE: Determine whether increases in hydration of thoracic and cervical IVDs occur with short-duration recumbency...
June 9, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28603956/activatable-t1-relaxivity-recovery-nanoconjugates-for-kinetic-and-sensitive-analysis-of-matrix-metalloprotease-2
#8
Xianglong Zhu, Hongyu Lin, Lirong Wang, Xiaoxue Tang, Lengceng Ma, Zhong Chen, Jinhao Gao
Sensitive detection of Matrix metalloproteinase 2 (MMP-2, an important cancer marker associated with tumor invasion and metastasis) activity in vitro and at cellular level is of great significance to clinical diagnosis and medical treatment. With unique physical properties, nanoparticles are emerging as a platform to construct conjugates of various biological molecules, which can be expected to generate new types of biosensors. In this work, Fe3O4 NPs were modified with Gd chelates via linking peptides to construct NP-substrate (Fe3O4-pep-Gd) conjugates for kinetic MMP-2 activity assessment in vitro, at cellular level and in vivo...
June 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28600230/cytokine-and-chemokine-profile-changes-in-patients-with-lower-segment-lumbar-degenerative-spondylolisthesis
#9
Juraj Sutovsky, Martin Benco, Martina Sutovska, Michaela Kocmalova, Lenka Pappova, Juraj Miklusica, Andrej Frano, Egon Kurca
BACKGROUND: Lumbar degenerative spondylolisthesis (DS) develops as a result of inflammatory and remodeling processes in facet joints (FJs). Several inflammatory cytokines are involved in the osteoarthritic and remodeling changes that occur and in low-back and/or radicular pain, the most prevalent clinical symptom of disease. This study improves knowledge related to the roles that 27 cytokines, chemokines and growth factors play in the pathophysiology of lumbar DS. MATERIAL AND METHODS: Cytokine levels were examined using capture sandwich immunoassay using the Bio-Plex(®) 200 System and the Bio-PlexTM Human Cytokine Standard 27-Plex, Group I (Bio-Rad, Hercules, California, USA) separately in intervertebral discs (IVDs) and FJ bone tissue...
June 6, 2017: International Journal of Surgery
https://www.readbyqxmd.com/read/28599678/the-prevalence-of-dengue-virus-serotypes-in-asymptomatic-blood-donors-reveals-the-emergence-of-serotype-4-in-saudi-arabia
#10
Ahmed Mohamed Ashshi
BACKGROUND: Transmission of dengue virus (DENV) through blood transfusion has been documented and hence screening for DENV during blood donation has been recently recommended by the American Association of Blood Banks and Centres of Disease Control and Prevention. DENV is endemic in the Western province of the Kingdom of Saudi Arabia (KSA) and serotypes 1, 2 and 3, but not 4, have been detected. However, little is known regarding the rates of DENV during blood donation in the kingdom...
June 9, 2017: Virology Journal
https://www.readbyqxmd.com/read/28596563/the-performance-of-the-sept9-gene-methylation-assay-and-a-comparison-with-other-crc-screening-tests-a-meta-analysis
#11
Lele Song, Jia Jia, Xiumei Peng, Wenhua Xiao, Yuemin Li
The SEPT9 gene methylation assay is the first FDA-approved blood assay for colorectal cancer (CRC) screening. Fecal immunochemical test (FIT), FIT-DNA test and CEA assay are also in vitro diagnostic (IVD) tests used in CRC screening. This meta-analysis aims to review the SEPT9 assay performance and compare it with other IVD CRC screening tests. By searching the Ovid MEDLINE, EMBASE, CBMdisc and CJFD database, 25 out of 180 studies were identified to report the SEPT9 assay performance. 2613 CRC cases and 6030 controls were included, and sensitivity and specificity were used to evaluate its performance at various algorithms...
June 8, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28594131/optimization-of-puncture-injury-to-rat-caudal-disc-for-mimicking-early-degeneration-of-intervertebral-disc
#12
Ming-Hsiao Hu, Kai-Chiang Yang, Yeong-Jang Chen, Yuan-Hui Sun, Feng-Huei Lin, Shu-Hua Yang
The caudal discs of rats have been proposed as a puncture model in which intervertebral disc (IVD) degeneration can be induced and novel therapies can be tested. For biological repair, treatments for ongoing IVD degeneration are ideally administered during the earlier stages. The purpose of this study was to elucidate the optimal puncture needle size for creating a model that mimicked the earlier stages of IVD degeneration. According to the disc height index, histologic score and MRI grading, a puncture needle sized 21G or larger induced rapid degenerative processes in rat caudal discs during the initial 2-4 weeks...
June 8, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28587986/angle-ply-biomaterial-scaffold-for-annulus-fibrosus-repair-replicates-native-tissue-mechanical-properties-restores-spinal-kinematics-and-supports-cell-viability
#13
Ryan Borem, Allison Madeline, Joshua Walters, Henry Mayo, Sanjitpal Gill, Jeremy Mercuri
Annulus fibrosus (AF) damage commonly occurs due to intervertebral disc (IVD) degeneration/herniation. The dynamic mechanical role of the AF is essential for proper IVD function and thus it is imperative that biomaterials developed to repair the AF withstand the mechanical rigors of the native tissue. Furthermore, these biomaterials must resist accelerated degradation within the proteolytic environment of degenerate IVDs while supporting integration with host tissue. We have previously reported a novel approach for developing collagen-based, multi-laminate AF repair patches (AFRPs) that mimic the angle-ply architecture and basic tensile properties of the human AF...
June 3, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28586059/identification-of-critical-genes-in-nucleus-pulposus-cells-isolated-from-degenerated-intervertebral-discs-using-bioinformatics-analysis
#14
Zhuangchen Zhu, Guang Chen, Wei Jiao, Defeng Wang, Yan Cao, Qingfu Zhang, Junqin Wang
Intervertebral disc (IVD) degeneration is a pathological process, which may lead to lower back pain. The present study aimed to investigate the pathogenesis of IVD degeneration. GSE42611 was downloaded from Gene Expression Omnibus, including 4 nucleus pulposus samples isolated from degenerated IVDs and 4 nucleus pulposus samples separated from normal IVDs. The differentially expressed genes (DEGs) between the degenerated and normal samples were screened using the limma package in R. Functional and pathway enrichment analyses were conducted separately for the upregulated and downregulated genes, using Database for Annotation, Visualization and Integrated Discovery software...
July 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28586025/protective-role-of-17%C3%AE-estradiol-on-tumor-necrosis-factor-%C3%AE-induced-apoptosis-in-human-nucleus-pulposus-cells
#15
Huan Liu, Si-Dong Yang, Ying Xu, Sheng-Hua Ning, Tao Wang, Da-Long Yang, Wen-Yuan Ding
The molecular mechanisms underlying protection and pathogenesis in spinal degenerative diseases remain unclear. Tumor necrosis factor-α (TNF-α) has been demonstrated to induce apoptosis of inte rvertebral disc (IVD) cells during IVD degeneration, and 17β‑estradiol (17β‑E2) has a protective effect against IVD cell apoptosis. However, the underlying molecular mechanism by which 17β‑E2 protects nucleus pulposus (NP) cells remains to be investigated. The aim of the present study was to evaluate whether 17β‑E2 modulates apoptosis of human NP cells induced by TNF‑α...
June 6, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28582905/a-comparative-study-on-the-mechanical-behavior-of-intervertebral-disc-using-hyperelastic-finite-element-model
#16
Feng Xie, Honghai Zhou, Wenju Zhao, Lixin Huang
BACKGROUND: Lumbar disc herniation may result in excruciating pain due to it being one of the most common diseases related to changes of intervertebral disc (IVD). In order to find a better clinical treatment and prevention scheme for relieving the pain caused by spine degeneration, the mechanical behavior analysis of IVD must be studied. OBJECTIVE: A finite element method (FEM) is used in this study to analyze the mechanical behaviors of healthy and herniated IVD...
May 19, 2017: Technology and Health Care: Official Journal of the European Society for Engineering and Medicine
https://www.readbyqxmd.com/read/28582742/bioaccessibility-of-pahs-in-contaminated-soils-comparison-of-five-in-vitro-methods-with-tenax-as-a-sorption-sink
#17
Shujun Zhang, Chao Li, Yunzi Li, Ruirui Zhang, Peng Gao, Xinyi Cui, Lena Q Ma
For hydrophobic organic contaminants, physiologically based in vitro methods may need to include a sorption sink to simulate in vivo intestinal uptake. We compared PAH bioaccessibility in contaminated soils using five in vitro methods including physiologically based extraction test (PBET), in vitro digestion assay (IVD), method from Deutsches Institut für Normung (DIN), unified bioaccessibility method (UBM), and fed organic estimation human simulation test (FOREhST) in the absence and presence of Tenax as a sorption sink...
June 2, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28576140/rank-rankl-opg-system-in-the-intervertebral-disc
#18
Norihiko Takegami, Koji Akeda, Junichi Yamada, Tomohiko Sano, Koichiro Murata, Jenny Huang, Koichi Masuda, Akihiro Sudo
BACKGROUND: The receptor activator of NF-κB ligand (RANKL), a member of the TNF ligand superfamily, is known to regulate bone metabolism. The expression of each component of the RANK/RANKL/osteoprotegerin (OPG) system in the intervertebral disc (IVD) has not been examined in detail. The purposes of this study were to examine the expression of the RANK/RANKL/OPG system and to evaluate the function of RANKL in the matrix metabolism of the rat IVD. METHODS: Sprague-Dawley, 12-week-old, male rats were used in this study...
June 2, 2017: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/28570641/correlation-study-between-facet-joint-cartilage-and-intervertebral-discs-in-early-lumbar-vertebral-degeneration-using-t2-t2-and-t1%C3%AF-mapping
#19
Yi Zhang, Jianzhong Hu, Chunyue Duan, Ping Hu, Hongbin Lu, Xianjing Peng
Recent advancements in magnetic resonance imaging have allowed for the early detection of biochemical changes in intervertebral discs and articular cartilage. Here, we assessed the feasibility of axial T2, T2* and T1ρ mapping of the lumbar facet joints (LFJs) to determine correlations between cartilage and intervertebral discs (IVDs) in early lumbar vertebral degeneration. We recruited 22 volunteers and examined 202 LFJs and 101 IVDs with morphological (sagittal and axial FSE T2-weighted imaging) and axial biochemical (T2, T2* and T1ρ mapping) sequences using a 3...
2017: PloS One
https://www.readbyqxmd.com/read/28567592/transcriptional-profiling-distinguishes-inner-and-outer-annulus-fibrosus-from-nucleus-pulposus-in-the-bovine-intervertebral-disc
#20
Guus G H van den Akker, Marije I Koenders, Fons A J van de Loo, Peter L E M van Lent, Esmeralda Blaney Davidson, Peter M van der Kraan
BACKGROUND: Cells in the intervertebral disc have unique phenotypes and marker genes that separate the nucleus pulposus (NP), annulus fibrosus (AF) and articular cartilage (AC) have been identified. Recently, it was shown that phenotypic marker genes exhibit variable expression in humans. In this study, the bovine tail was used to determine the ability of marker genes to distinguish the outer and inner AF from NP tissue and isolated cells. METHODS: Bovine tail intervertebral discs from 13 donors were dissected and correct isolation of tissue was confirmed...
May 31, 2017: European Spine Journal
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