Read by QxMD icon Read


Carla Cunha, Catarina R Almeida, Maria Inês Almeida, Andreia M Silva, Maria Molinos, Sofia Lamas, Catarina L Pereira, Graciosa Q Teixeira, António T Monteiro, Susana G Santos, Raquel M Gonçalves, Mário A Barbosa
: : Cell therapies for intervertebral disc (IVD) regeneration presently rely on transplantation of IVD cells or stem cells directly to the lesion site. Still, the harsh IVD environment, with low irrigation and high mechanical stress, challenges cell administration and survival. In this study, we addressed systemic transplantation of allogeneic bone marrow mesenchymal stem cells (MSCs) intravenously into a rat IVD lesion model, exploring tissue regeneration via cell signaling to the lesion site...
October 11, 2016: Stem Cells Translational Medicine
Rafael Menezes-Reis, Carlos E Garrido Salmon, Gustavo P Bonugli, Debora Mazoroski, Mauricio H Tamashiro, Leonor G Savarese, Marcello Henrique Nogueira-Barbosa
BACKGROUND: To investigate the detection of intervertebral disc (IVD) composition aging-related changes using T2 and T1ρ relaxometry in vivo in asymptomatic young adults. METHODS: We recruited ninety asymptomatic and young adults (42 men and 48 women) between 20 and 40 years old. T2 and T1ρ lumbar spine mappings were acquired using 1.5 T magnetic resonance imaging (MRI) scanner. Two independent observers manually segmented 450 lumbar discs in all slices. They also performed sub region segmentation of annulus fibrosus (AF) and nucleus pulposus (NP) at the central MRI sagittal slices...
August 2016: Quantitative Imaging in Medicine and Surgery
Urška Potočar, Samo Hudoklin, Mateja Erdani Kreft, Janja Završnik, Krešimir Božikov, Mirjam Fröhlich
Cell therapies present a feasible option for the treatment of degenerated cartilaginous and intervertebral disc (IVD) tissues. Microenvironments of these tissues are specific and often differ from the microenvironment of cells that, could be potentially used for therapy, e.g. human adipose-derived stem cells (hASC). To ensure safe and efficient implantation of hASC, it is important to evaluate how microenvironmental conditions at the site of implantation affect the implanted cells. This study has demonstrated that cartilaginous tissue-specific osmolarities ranging from 400-600 mOsm/L affected hASC in a dose- and time-dependent fashion in comparison to 300 mOsm/L...
2016: PloS One
Mohamed Derbel, Dhia Mekki, Abir Riahi, Kaouther Dimassi, Mhamed Sami Mebazaa, Mohamed Faouzi Gara
Background Although Oxytocin is used systematically during caesarean section, no recommendation precise optimal dose for this indication. The bolus administration of 10 IU intravenously after extraction of the newborn, is accompanied by side effects mainly hemodynamic. Objectives To compare two Oxytocin protocols:  05 IU bolus Vs. 10 IU bolus during Cesarean section by studying their respective effectiveness (effects on uterine tone) and adverse effects. Methods A prospective randomized double-blind study including 87 term parturients for undergoing a C-section under loco regional anesthesia...
April 2016: La Tunisie Médicale
Chenglin Sang, Xuecheng Cao, Fangjing Chen, Xuekang Yang, Yongxian Zhang
Objective. Nucleus pulposus (NP) and annulus fibrosus (AF) are two main components of intervertebral disc (IVD). We aimed to figure out whether NP and AF also contain stem cells and whether these stem cells share common properties with chondrocytes and/or fibroblasts in their phenotypes or whether they are completely different types of cells with different characteristics. Design. The disk cells were isolated from AF and NP tissues of the same lumbar spine of the rabbits. The properties of these disk cells were characterized by their morphology, population doubling time (PDT), stem cell marker expression, and multidifferentiation potential using tissue culture techniques, immunocytochemistry, and RT-PCR...
2016: Stem Cells International
Christoph Schleich, Falk Miese, Anja Müller-Lutz, Johannes Boos, Joel Aissa, Adrian Nasca, Frank Zaucke, Tamara Bostelmann, Lars Schimmöller, Bernd Bittersohl, Hans-Jakob Steiger, Gerald Antoch, Richard Bostelmann
The study was performed to preoperatively assess the cartilage integrity of cervical intervertebral discs (IVDs) using Delayed Gadolinium-Enhanced Magnetic Resonance Imaging of Cartilage (dGEMRIC). Therefore, 53 cervical intervertebral discs of nine preoperative patients with neck and shoulder/arm pain scheduled for discectomy (five females, four males; mean age: 47.1 ± 8.4 years; range: 36-58 years) were included for biochemical analysis in this retrospective study. The patients underwent 3T magnetic resonance imaging (MRI) including biochemical imaging with dGEMRIC and morphological, sagittal T2 weighted (T2w) imaging...
October 4, 2016: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Shady Elmasry, Shihab Asfour, Juan Pablo de Rivero Vaccari, Francesco Travascio
Insulin-like growth factor-1 (IGF-1) is well-known for upregulating cell proliferation and biosynthesis of the extracellular matrix in the intervertebral disc (IVD). Pathological conditions, such as obesity or chronic kidney disease cause IGF-1 deficiency in plasma. How this deficiency impacts disc homeostasis remains unknown. Pro-anabolic approaches for the treatment of disc degeneration based on enhancing IGF-1 bioavailability to tissue-cells are considered, but knowledge of their effectiveness in enhancing cellular anabolism of a degenerated disc is limited...
September 22, 2016: Computers in Biology and Medicine
Naimisha Beeravolu, Jared Brougham, Irfan Khan, Christina McKee, Mick Perez-Cruet, Rasul G Chaudhry
Intervertebral disc (IVD) degeneration is characterized by the loss of nucleus pulposus (NP), which is a common cause for lower back pain. Although, currently, there is no cure for the degenerative disc disease (DDD), stem cell therapy is increasingly being considered for its treatment. In this study, we investigated the feasibility and efficacy of human umbilical cord mesenchymal stem cells (UC-MSCs) and chondroprogenitor cells (CPCs) derived from those cells to regenerate damaged IVD in a rabbit model. Transplanted cells survived, engrafted and dispersed into nucleus pulposus (NP) in situ...
September 30, 2016: Journal of Tissue Engineering and Regenerative Medicine
Dave Armbruster, James Donnelly
At the start of the 21st century, a dramatic change occurred in the clinical laboratory community. Concepts from Metrology, the science of measurement, began to be more carefully applied to the in vitro diagnostic (IVD) community, that is, manufacturers. A new appreciation of calibrator traceability evolved. Although metrological traceability always existed, it was less detailed and formal. The In Vitro Diagnostics Directive (IVDD) of 2003 required manufacturers to provide traceability information, proving assays were anchored to internationally accepted reference materials and/or reference methods...
February 2016: EJIFCC
Liulin Luo, Weijia Liu, Bing Li, Mengling Li, Dongdong Huang, Linjie Jing, Huiping Chen, Jinghui Yang, Jun Yue, Fang Wang, Haiqing Chu, Zhemin Zhang
This study was undertaken to evaluate the utility of matrix-assisted laser desorption ionization-time of flight mass spectrometry with the Vitek MS Plus system for identifying Mycobacterium abscessus subspecies in order to facilitate more rapid and appropriate therapy. A total of 175 clinical M. abscessus strains were identified by whole genome sequencing analysis: 139 M. abscessus subsp. abscessus and 36 M. abscessus subsp. massiliense The research-use-only (RUO) Saramis Knowledge Base database V.4.12 was modified accordingly by adding 40 M...
September 28, 2016: Journal of Clinical Microbiology
Simon Wan, Samantha Borland, Stephen M Richardson, Catherine L R Merry, Alberto Saiani, Julie E Gough
: Cell-based therapies for regeneration of intervertebral discs are regarded to hold promise for degenerative disc disease treatment, a condition that is strongly linked to lower back pain. A de novo self-assembling peptide hydrogel (SAPH), chosen for its biocompatibility, tailorable properties and nanofibrous architecture, was investigated as a cell carrier and scaffold for nucleus pulposus (NP) tissue engineering. Oscillatory rheology determined that the system would likely be deliverable via minimally invasive procedure and mechanical properties could be optimised to match the stiffness of the native human NP...
September 24, 2016: Acta Biomaterialia
Nizar Algarni, Michael Grant, Laura M Epure, Omar Salem, Rakan Farouk Bokhari, John Antoniou, Fackson Mwale
Link N (DHLSDNYTLDHDRAIH), is a naturally occurring peptide that can stimulate matrix production in vitro, in vivo and in intact ex vivo human intervertebral discs (IVDs). We have recently discovered that annulus fibrosus cells (AF) cells have the ability to proteolytically process Link N resulting in a fragment spanning amino acid residues 1-8 short Link N (sLink N). Separately, we recently developed and validated a large animal disc organ culture model that maintains long-term cell viability while retaining the bony endplates and vertebral bone (vIVDs; IVD with intact vertebrae)...
September 27, 2016: Tissue Engineering. Part A
Zhengwei Zhou, Maxim Bez, Wafa Tawackoli, Joseph Giaconi, Dmitriy Sheyn, Sandra de Mel, Marcel M Maya, Barry D Pressman, Zulma Gazit, Gadi Pelled, Dan Gazit, Debiao Li
PURPOSE: Previous studies have associated low pH in intervertebral discs (IVDs) with discogenic back pain. The purpose of this study was to determine whether quantitative CEST (qCEST) MRI can be used to detect pH changes in IVDs in vivo. METHODS: The exchange rate ksw between glycosaminoglycan (GAG) protons and water protons was determined from qCEST analysis. Its dependence on pH value was investigated in GAG phantoms with varying pH and concentrations. The relationship between ksw and pH was studied further in vivo in a porcine model on a 3T MR scanner and validated using a pH meter...
September 26, 2016: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
S Ye, B Ju, H Wang, K-B Lee
OBJECTIVES: Interleukin 18 (IL-18) is a regulatory cytokine that degrades the disc matrix. Bone morphogenetic protein-2 (BMP-2) stimulates synthesis of the disc extracellular matrix. However, the combined effects of BMP-2 and IL-18 on human intervertebral disc degeneration have not previously been reported. The aim of this study was to investigate the effects of the anabolic cytokine BMP-2 and the catabolic cytokine IL-18 on human nucleus pulposus (NP) and annulus fibrosus (AF) cells and, therefore, to identify potential therapeutic and clinical benefits of recombinant human (rh)BMP-2 in intervertebral disc degeneration...
September 2016: Bone & Joint Research
Cheng Hui Yang, Tung-Hsin Wu, Chung-Jung Lin, Yi-You Chiou, Ying-Chou Chen, Ming-Huei Sheu, Wan-Yuo Guo, Chen Fen Chiu
PURPOSE: To evaluate the image quality and diagnostic confidence of reduced-dose computed tomography (CT) of the lumbar spine (L-spine) reconstructed with knowledge-based iterative model reconstruction (IMR). MATERIALS AND METHODS: Prospectively, group A consisted of 55 patients imaged with standard acquisition reconstructed with filtered back-projection. Group B consisted of 58 patients imaged with half tube current, reconstructed with hybrid iterative reconstruction (iDose(4)) in Group B1 and knowledge-based IMR in Group B2...
October 2016: European Journal of Radiology
Harald Enzmann, Ralf Meyer, Karl Broich
The new European regulation for in vitro diagnostics (IVD) divides the certification of IVD including companion diagnostics (CDx) by notified bodies (NB) from the market authorization of medicines. With the new regulation, CDx will require conformity assessment which is expected to include clinical evidence by NB. This is a significant change from the current situation: until now most IVD have been certified based on their manufacturers' assessment. For one medicine assessed by the EMA, certification of several different CDx by different NB is possible...
September 23, 2016: Biomarkers in Medicine
Li-Guo Zhu, Min-Shan Feng, Jia-Wen Zhan, Ping Zhang, Jie Yu
BACKGROUND: The development of mechanically active culture systems helps increase the understanding of the role of mechanical stress in intervertebral disc (IVD) degeneration. Motion segment cultures allow for preservation of the native IVD structure, and adjacent vertebral bodies facilitate the application and control of mechanical loads. The purpose of this study was to establish loading and organ culture methods for rabbit IVD motion segments to study the effect of static load on the whole disc organ...
2016: Chinese Medical Journal
Elsbeth Heinzelmann
In vitro diagnostics (IVD) has huge potential. Primary drivers in the global market are the patient's awareness of infectious diseases, the introduction of advanced molecular and tissue diagnostic tests for patient-stratified and targeted anti-cancer therapy and, last but not least, the growing geriatric population. Rapid progress in device miniaturization and information technology (IT) offers new possibilities in decentralized testing. Grand View Research Inc. expects the global market for IVD to reach US $ 74...
September 2016: Chimia
Heshmatollah Ghanbari, Farzan Kianersi, Seyed Ali Sonbolestan, Mohammad-Ali Abtahi, Mojataba Akbari, Zahra-Alsadat Abtahi, Seyed-Hossein Abtahi
The aim of this study is to evaluate the short-term effects of a single intravitreal injection of 1.25 mg Bevacizumab combined with 300 lg/0.1 mL Diclofenac (IVB/D) versus 1.25 mg intravitreal Bevacizumab (IVB) alone in the treatment of naive diabetic macular edema (DME). In this prospective, randomized clinical trial, 80 eyes were included in the final analysis; 42 and 38 of which in the IVB and IVB/D groups, respectively. The primary outcome measure was a change in best-corrected visual acuity (BCVA) in logMAR at week 4...
September 13, 2016: International Ophthalmology
Young-Mi Kang, Seong-Hwan Hong, Jae-Ho Yang, Jin-Cheol Oh, Jin-Oh Park, Byung Ho Lee, Sang-Yoon Lee, Hak-Sun Kim, Hwan-Mo Lee, Seong-Hwan Moon
BACKGROUND: N-containing bisphosphonates (BPs), such as pamidronate and risedronate, can inhibit osteoclastic function and reduce osteoclast number by inducing apoptotic cell death in osteoclasts. The aim of this study is to demonstrate the effect of pamidronate, second generation nitrogen-containing BPs and to elucidate matrix metallo-proteinases (MMPs) mRNA expression under serum starvation and/or tumor necrosis factor alpha (TNF-α) stimulation on metabolism of intervertebral disc (IVD) cells in vitro...
August 2016: Journal of Bone Metabolism
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"