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https://www.readbyqxmd.com/read/28448052/an-in-vitro-organ-culture-model-of-the-murine-intervertebral-disc
#1
Jennifer W Liu, Kevin H Lin, Christian Weber, Sameer Bhalla, Sean Kelso, Kaixi Wang, Simon Y Tang
Intervertebral disc (IVD) degeneration is a significant contributor to low back pain. The IVD is a fibrocartilaginous joint that serves to transmit and dampen loads in the spine. The IVD consists of a proteoglycan-rich nucleus pulposus (NP) and a collagen-rich annulus fibrosis (AF) sandwiched by cartilaginous end-plates. Together with the adjacent vertebrae, the vertebrae-IVD structure forms a functional spine unit (FSU). These microstructures contain unique cell types as well as unique extracellular matrices...
April 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447513/clinical-utility-of-different-anti-m%C3%A3-llerian-hormone-amh-assays-for-the-purpose-of-pregnancy-prediction
#2
Joanna Liss, Michal Kunicki, Adam Czyzyk, Ewa Pastuszek, Judyta Zabielska, Blazej Meczekalski, Krzysztof Lukaszuk
PURPOSE: Comparison of outcomes of IVF cycles where the AMH levels was measured with five different AMH kits: Immunotech (IOT), Beckman Coulter II Gen. RUO, Beckman Coulter II Gen. IVD (BC II IVD), Ansh Labs ultrasensitive (Ansh) and the automated Elecsys Roche assay. METHODS: Retrospective analysis of clinical data for 3693 cycles. RESULTS: In women < 35 years with low (<0.6 ng/ml) and high (>1.4 ng/ml) AMH concentrations, and in those > 39 years with medium (≥0...
April 27, 2017: Gynecological Endocrinology
https://www.readbyqxmd.com/read/28442757/comparison-of-circulating-tumour-cells-and-circulating-cell-free-epstein-barr-virus-dna-in-patients-with-nasopharyngeal-carcinoma-undergoing-radiotherapy
#3
Jess Honganh Vo, Wen Long Nei, Min Hu, Wai Min Phyo, Fuqiang Wang, Kam Weng Fong, Terence Tan, Yoke Lim Soong, Shie Lee Cheah, Kiattisa Sommat, Huiyu Low, Belinda Ling, Johnson Ng, Wan Loo Tan, Kian Sing Chan, Lynette Oon, Jackie Y Ying, Min-Han Tan
Quantification of Epstein-Barr virus (EBV) cell-free DNA (cfDNA) is commonly used in clinical settings as a circulating biomarker in nasopharyngeal carcinoma (NPC), but there has been no comparison with circulating tumour cells (CTCs). Our study aims to compare the performance of CTC enumeration against EBV cfDNA quantitation through digital PCR (dPCR) and quantitative PCR. 74 plasma samples from 46 NPC patients at baseline and one month after radiotherapy with or without concurrent chemotherapy were analysed...
December 19, 2016: Scientific Reports
https://www.readbyqxmd.com/read/28442046/morphometric-developmental-pattern-of-bovine-handmade-cloned-concepti-in-late-pregnancy
#4
R P C Gerger, F C Zago, E S Ribeiro, S Gaudencio Neto, L T Martins, L H Aguiar, V H V Rodrigues, F H Furlan, I Ortigari, R D Sainz, C L Ferrell, M A Miglino, C E Ambrósio, J L Rodrigues, R Rossetto, F Forell, L R Bertolini, M Bertolini
Cloning procedures often interfere with conceptus growth and life ex utero, in a set of symptoms known as abnormal offspring syndrome (AOS). The aim of the present study was to compare the developmental pattern of in vivo-derived (IVD), IVF-derived and handmade cloning-derived (NT-HMC) Day 225 bovine concepti using established procedures. Pregnancy diagnosis was performed on Day 30 following blastocyst transfer on Day 7. Conceptus morphometry was assessed by ultrasonography on Day 51, and on Day 225 pregnant cows were killed for morphological examination of concepti...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28433788/regulation-of-human-nucleus-pulposus-cells-by-peptide-coupled-substrates
#5
Devin T Bridgen, Bailey V Fearing, Liufang Jing, Johannah Sanchez-Adams, Megan C Cohan, Farshid Guilak, Jun Chen, Lori A Setton
Nucleus pulposus (NP) cells are derived from the notochord and differ from neighboring cells of the intervertebral disc in phenotypic marker expression and morphology. Adult human NP cells lose this phenotype and morphology with age in a pattern that contributes to progressive disc degeneration and pathology. Select laminin-mimetic peptide ligands and substrate stiffnesses were examined for their ability to regulate human NP cell phenotype and biosynthesis through the expression of NP-specific markers aggrecan, N-cadherin, collagen types I and II, and GLUT1...
April 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28430387/age-related-reduction-in-the-expression-of-foxo-transcription-factors-and-correlations-with-intervertebral-disc-degeneration
#6
Oscar Alvarez-Garcia, Tokio Matsuzaki, Merissa Olmer, Koichi Masuda, Martin K Lotz
Aging is a main risk factor for intervertebral disc (IVD) degeneration, the main cause of low back pain. FOXO transcription factors are important regulators of tissue homeostasis and longevity. Here, we determined the expression pattern of FOXO in healthy and degenerated human IVD and the associations with IVD degeneration during mouse aging. FOXO expression was assessed by immunohistochemistry in normal and degenerated human IVD samples and in cervical and lumbar IVD from 6, 12, 24, and 36-month-old C57BL/6J mice...
April 21, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
https://www.readbyqxmd.com/read/28427233/assessment-of-the-interlaboratory-variability-and-robustness-of-jak2v617f-mutation-assays-a-study-involving-a-consortium-of-19-italian-laboratories
#7
Margherita Perricone, Francesca Palandri, Emanuela Ottaviani, Mario Angelini, Laura Bagli, Enrica Bellesia, Meris Donati, Donato Gemmati, Patrizia Zucchini, Stefania Mancini, Valentina Marchica, Serena Trubini, Giovanna De Matteis, Silvia Di Zacomo, Mosè Favarato, Annamaria Fioroni, Caterina Bolzonella, Giorgia Maccari, Filippo Navaglia, Daniela Gatti, Luisa Toffolatti, Linda Orlandi, Vèronique Laloux, Marco Manfrini, Piero Galieni, Barbara Giannini, Alessia Tieghi, Sara Barulli, Maria Luisa Serino, Monica Maccaferri, Anna Rita Scortechini, Nicola Giuliani, Daniele Vallisa, Massimiliano Bonifacio, Patrizia Accorsi, Cristina Salbe, Vinicio Fazio, Milena Gusella, Eleonora Toffoletti, Marzia Salvucci, Mirija Svaldi, Filippo Gherlinzoni, Francesca Cassavia, Francesco Orsini, Giovanni Martinelli
To date, a plenty of techniques for the detection of JAK2V617F is used over different laboratories, with substantial differences in specificity and sensitivity. Therefore, to provide reliable and comparable results, the standardization of molecular techniques is mandatory.A network of 19 centers was established to 1) evaluate the inter- and intra-laboratory variability in JAK2V617F quantification, 2) identify the most robust assay for the standardization of the molecular test and 3) allow consistent interpretation of individual patient analysis results...
March 6, 2017: Oncotarget
https://www.readbyqxmd.com/read/28424252/comparison-of-saramis-4-12-and-ivd-3-0-vitek-ms-matrix-assisted-laser-desorption-ionization-time-of-flight-mass-spectrometry-for-identification-of-mycobacteria-from-solid-and-liquid-culture-media
#8
Caroline Leyer, Guillaume Gregorowicz, Faiza Mougari, Laurent Raskine, Emmanuelle Cambau, Dominique de Briel
During the last decade, many publications studied the matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for mycobacterial identification. Diverse and contradictory results indicated that optimal level for routine testing has not been reached yet. This work aimed to assess Vitek MS through two distinct versions, Saramis v4.12 RUO and the IVD v3.0, under conditions close to routine laboratory practice. Overall, 111 mycobacterial isolates were submitted to protein extraction and same spectra were matched against both databases...
April 19, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28422845/response-to-benzodiazepines-and-the-clinical-course-in-malignant-catatonia-associated-with-schizophrenia-a-case-report
#9
Kazutaka Ohi, Aki Kuwata, Takamitsu Shimada, Toshiki Yasuyama, Yusuke Nitta, Takashi Uehara, Yasuhiro Kawasaki
BACKGROUND: Malignant catatonia (MC) is a disorder consisting of catatonic symptoms, hyperthermia, autonomic instability, and altered mental status. Neuroleptic malignant syndrome (NMS) caused by antipsychotics is considered a variant of MC. Benzodiazepine (BZD) medications are safe and effective treatments providing rapid relief from MC. This case study reports a detailed clinical course of a case of MC associated with schizophrenia initially diagnosed as NMS that responded successfully to BZDs but not to dantrolene...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28422800/evaluation-of-water-content-in-lumbar-intervertebral-discs-and-facet-joints-before-and-after-physiological-loading-using-t2-mapping-mri
#10
Daisuke Yamabe, Hideki Murakami, Kou Chokan, Hirooki Endo, Ryosuke Oikawa, Shoitsu Sawamura, Minoru Doita
STUDY DESIGN: T2 mapping was used to quantify the water content of lumbar spine intervertebral discs (IVD) and facet joints before and after physiological loading. OBJECTIVE: To clarify the interaction between lumbar spine IVD and facet joints as load-bearing structures by measuring the water content of their matrix after physiological loading using T2 mapping magnetic resonance imaging (MRI). SUMMARY OF BACKGROUND DATA: To date, few reports have functionally evaluated lumbar spine IVD and facet joints, and their interaction in vivo...
April 18, 2017: Spine
https://www.readbyqxmd.com/read/28422125/running-exercise-strengthens-the-intervertebral-disc
#11
Daniel L Belavý, Matthew J Quittner, Nicola Ridgers, Yuan Ling, David Connell, Timo Rantalainen
There is currently no evidence that the intervertebral discs (IVDs) can respond positively to exercise in humans. Some authors have argued that IVD metabolism in humans is too slow to respond anabolically to exercise within the human lifespan. Here we show that chronic running exercise in men and women is associated with better IVD composition (hydration and proteoglycan content) and with IVD hypertrophy. Via quantitative assessment of physical activity we further find that accelerations at fast walking and slow running (2 m/s), but not high-impact tasks, lower intensity walking or static positions, correlated to positive IVD characteristics...
April 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28403956/severe-perineal-morbidity-of-instrumental-deliveries-using-thierry-s-spatulas-and-vacuum-extraction-a-prospective-observational-cohort-study
#12
S Hamouda, J Mancini, F Marchand, F Bretelle, L Boubli, C D'Ercole, X Carcopino
OBJECTIVES: To evaluate the risk of severe perineal tear following instrumental vaginal delivery (IVD) performed with spatulas and vacuum extraction. Secondary objectives were to estimate the impact of episiotomy on this risk. METHODS: From December 2008 to October 2012, women who underwent spatulas or vacuum were prospectively included. Each spontaneous vaginal delivery (SVD) following each included IVD were included as control cases (1-1 ratio). Careful perineal examination was systematically performed...
January 2017: J Gynecol Obstet Hum Reprod
https://www.readbyqxmd.com/read/28402966/an-injectable-nucleus-pulposus-cell-modified-decellularized-scaffold-biocompatible-material-for-prevention-of-disc-degeneration
#13
Zhi Shan, Xianfeng Lin, Shengyu Wang, Xuyang Zhang, Yichuan Pang, Shengyun Li, Tianming Yu, Shunwu Fan, Fengdong Zhao
We developed a nucleus pulposus cell (NPC)-modulated decellularized small intestinal submucosa (SIS) scaffold, and assessed the ability of this material to prevent Intervertebral disc degeneration (IVD) degeneration. Decellularized porcine SIS was squashed into particles and the biological safety and efficiency of these particles were evaluated. Next, SIS particles were seeded with rabbit NPCs, cultured for two months in vitro, decellularized again and suspended for intervertebral injection. We demonstrated that use of the decellularization protocol resulted in the removal of cellular components with maximal retention of extracellular matrix...
April 4, 2017: Oncotarget
https://www.readbyqxmd.com/read/28396688/age-related-changes-in-nucleus-pulposus-mesenchymal-stem-cells-an-in-vitro-study-in-rats
#14
Yachao Zhao, Zhiwei Jia, Shanshan Huang, Yaohong Wu, Longgang Liu, Linghan Lin, Deli Wang, Qing He, Dike Ruan
The functions of mesenchymal stem cells (MSCs) appear to decline with age due to cellular senescence, which could reduce the efficacy of MSCs-based therapies. Recently, MSCs have been identified in the nucleus pulposus, which offers great potential for intervertebral disc (IVD) repair. However, this potential might be affected by the senescence of nucleus pulposus MSCs (NPMSCs), but whether or not this exists remains unknown. The aim of this study was to investigate the age-related changes in NPMSCs. NPMSCs isolated from young (3-month-old) and old (14-month-old) Sprague-Dawley rats were cultured in vitro...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28394321/the-role-of-angiopoietin-2-in-nucleus-pulposus-cells-during-human-intervertebral-disc-degeneration
#15
Kun Wang, Wei Liu, Yu Song, Xinghuo Wu, Yukun Zhang, Shuai Li, Yong Gao, Ji Tu, Yingle Liu, Cao Yang
Although evidence shows that intervertebral disc degeneration is generally characterized by angiogenesis, the role of angiopoietin has not been investigated. This study examined the presence of angiopoietin-1 (Ang-1) and angiopoietin-2 (Ang-2) within the native intervertebral disc (IVD) and elucidated their functions in the regulation of nucleus pulposus (NP) cells. Initial investigation of uncultured NP tissue revealed that Ang-1 and Ang-2 were expressed by native NP cells. Ang-2 expression was significantly increased in infiltrated and degenerate samples relative to normal samples...
April 10, 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
https://www.readbyqxmd.com/read/28392965/mechanosignaling-activation-of-tgf%C3%AE-maintains-intervertebral-disc-homeostasis
#16
Qin Bian, Lei Ma, Amit Jain, Janet L Crane, Khaled Kebaish, Mei Wan, Zhengdong Zhang, X Edward Guo, Paul D Sponseller, Cheryle A Séguin, Lee H Riley, Yongjun Wang, Xu Cao
Intervertebral disc (IVD) degeneration is the leading cause of disability with no disease-modifying treatment. IVD degeneration is associated with instable mechanical loading in the spine, but little is known about how mechanical stress regulates nucleus notochordal (NC) cells to maintain IVD homeostasis. Here we report that mechanical stress can result in excessive integrin αvβ6-mediated activation of transforming growth factor beta (TGFβ), decreased NC cell vacuoles, and increased matrix proteoglycan production, and results in degenerative disc disease (DDD)...
2017: Bone Research
https://www.readbyqxmd.com/read/28392887/ros-crucial-intermediators-in-the-pathogenesis-of-intervertebral-disc-degeneration
#17
REVIEW
Chencheng Feng, Minghui Yang, Minghong Lan, Chang Liu, Yang Zhang, Bo Huang, Huan Liu, Yue Zhou
Excessive reactive oxygen species (ROS) generation in degenerative intervertebral disc (IVD) indicates the contribution of oxidative stress to IVD degeneration (IDD), giving a novel insight into the pathogenesis of IDD. ROS are crucial intermediators in the signaling network of disc cells. They regulate the matrix metabolism, proinflammatory phenotype, apoptosis, autophagy, and senescence of disc cells. Oxidative stress not only reinforces matrix degradation and inflammation, but also promotes the decrease in the number of viable and functional cells in the microenvironment of IVDs...
2017: Oxidative Medicine and Cellular Longevity
https://www.readbyqxmd.com/read/28391818/a-computational-measurement-of-cartilaginous-endplate-structure-using-ultrashort-time-to-echo-mri-scanning
#18
Ri-Chu Jin, Yong-Can Huang, Keith D K Luk, Yong Hu
BACKGROUND AND OBJECTIVE: Ultrashort time-to-echo (UTE) MRI scanning has been applied to observe the cartilaginous endplate (CEP) in intervertebral disc. CEP plays a critical role in IVD health and disease. Nevertheless, current measurements of CEP based on UTE MRI technique are still by manual segmentation, and observation of signal abnormality was usually time-consuming and often disturbed by subjective bias. This study hence proposed an efficient way to harvest the global parameters of CEP after UTE MRI scanning...
May 2017: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/28386545/residual-spinal-cord-compression-following-hemilaminectomy-and-mini-hemilaminectomy-in-dogs-a-prospective-randomized-study
#19
Gustaf Svensson, Ulrika S H Simonsson, Fredrik Danielsson, Tobias Schwarz
The aim of this study was to compare the reduction of spinal cord compression after surgical treatment of dogs with acute thoracolumbar intervertebral disc (IVD) extrusion achieved using hemilaminectomy versus mini-hemilaminectomy techniques. This was a prospective randomized study with client-owned dogs presented with acute IVD extrusion that were allocated to surgical treatment using hemilaminectomy (n = 15) or mini-hemilaminectomy (n = 15) techniques. Plain and intravenous-contrast computed tomography was performed pre- and postoperatively...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/28386320/a-novel-approach-for-the-annulus-needle-puncture-model-of-intervertebral-disc-degeneration-in-rabbits
#20
Tao Lei, Yuan Zhang, Qiang Zhou, Xiaoji Luo, Ke Tang, Rongsheng Chen, Chang Yu, Zhengxue Quan
OBJECTIVE: To create the rabbit animal model of intervertebral disc (IVD) degeneration by the annulus needle puncture technique through a novel transabdominal approach. METHODS: Thirteen New Zealand White rabbits underwent annular puncture at the L3/4, L4/5, and L5/6 discs through a transabdominal approach. For a longitudinal study to assess changes in disc height over time, serial X-rays, T2-weighted magnetic resonance imaging (MRI) (T2WI), and T2 mapping MRI were performed pre-operation and at 2, 4, and 6 weeks after puncture...
2017: American Journal of Translational Research
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