Read by QxMD icon Read


Orit Chai, Tamar Harrosh, Tali Bdolah-Avram, Michal Mazaki-Tovi, Merav H Shamir
OBJECTIVE To characterize and identify risk factors for intervertebral disk extrusion (IVDE) in Pekingese. DESIGN Retrospective case-control study. ANIMALS 426 Pekingese (81 with confirmed IVDE and 345 without IVDE) admitted to a veterinary teaching hospital from 2005 through 2015. PROCEDURES Medical records were reviewed and data were extracted regarding age, sex, body weight, body condition score, coat color, chief reason for initial evaluation, body system involved, and diagnosis. Survey radiographs of the vertebral column were examined...
April 1, 2018: Journal of the American Veterinary Medical Association
Xiaoming Liu, Fumin Pan, Zhaoyu Ba, Shanjin Wang, Desheng Wu
BACKGROUND: Diabetes mellitus (DM) and low back pain which is mainly caused by degeneration of the intervertebral discs (IVDs) both are major public health problem worldwide. The present study was designed to investigate the association between type 2 diabetes mellitus (T2D) and severity of lumbar disc degeneration (LDD). METHODS: We retrospectively reviewed patients with low back pain visiting our spine clinic in 2014. Low back pain patients all have the lumbar MRI imaging and no previous treatment...
March 14, 2018: Journal of Orthopaedic Surgery and Research
Joonchul Shin, Sudesna Chakravarty, Wooseok Choi, Kyungyeon Lee, Dongsik Han, Hyundoo Hwang, Jaekyu Choi, Hyo-Il Jung
The emergence of a wide range of applications of smartphones along with advances in 'liquid biopsy' has significantly propelled medical research particularly in the field of in vitro diagnostics (IVD). Herein, we have presented a detailed analysis of IVD, its associated critical concerns and probable solutions. It also demonstrates the transition in terms of analytes from minimally invasive (blood) to non-invasive (urine, saliva and sweat) and depicts how the different features of a smartphone can be integrated for specific diagnostic purposes...
March 14, 2018: Analyst
Weiheng Wang, Yang Wang, Guoying Deng, Jun Ma, Xiaodong Huang, Jiangming Yu, Yanhai Xi, Xiaojian Ye
Objective: Special hypoxic and hypertonic microenvironment in intervertebral discs (IVDs) decreases the treatment effect of cell transplantation. We investigated the hypothesis that hypoxic preconditioning (HP) could improve the therapeutic effect of bone mesenchymal stem cells (BMSCs) to IVD degeneration. Methods: BMSCs from green fluorescent protein-transgenic rats were pretreated with cobalt chloride (CoCl2 , 100  μ M, 24 h) for hypoxic conditions in vitro ...
2018: Stem Cells International
Linghui Luo, Yong Gao, Cao Yang, Zengwu Shao, Xinghuo Wu, Shuai Li, Liming Xiong, Chao Chen
Most low back pain is caused by intervertebral discs (IVD) degeneration, a disease that prevalence is increasing with age. Halofuginone, an analog of ferbrifugine isolated from plant Dichroa febrifuga, has drawn much attention in recent years for the wide range of bioactivities in malaria, cancer, fibrotic and autoimmune diseases. In this study, we evaluated the benefit effects of halofuginone in IVD degeneration treatment in a validated rabbit puncture model. Halofuginone treatment could attenuate disc degeneration by suppressing the decrease of discs height and nucleus pulposus signal strength...
March 7, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ruhang Tang, Liufang Jing, Vincent P Willard, Chia-Lung Wu, Farshid Guilak, Jun Chen, Lori A Setton
BACKGROUND: Intervertebral disc (IVD) degeneration is characterized by an early decrease in cellularity of the nucleus pulposus (NP) region, and associated extracellular matrix changes, reduced hydration, and progressive degeneration. Cell-based IVD therapy has emerged as an area of great interest, with studies reporting regenerative potential for many cell sources, including autologous or allogeneic chondrocytes, primary IVD cells, and stem cells. Few approaches, however, have clear strategies to promote the NP phenotype, in part due to a limited knowledge of the defined markers and differentiation protocols for this lineage...
March 9, 2018: Stem Cell Research & Therapy
Xiaobin Wang, Mingxiang Zou, Jing Li, Bing Wang, Qianshi Zhang, Fubin Liu, Guohua Lü
Intervertebral disc (IVD) degeneration (IDD) is a major contributor to low back pain. During IDD progression, ROS can be produced in the form of H2 O2 in nucleus pulposus cells (NPCs) in response to elevated cytokines, leading to subsequent alternations of cell fate and metabolic processes. Genetic factors are considered as main contributors to IDD pathopoiesis. Herein, we investigated the detailed function and mechanism of H19, one of the most up-regulated lncRNAs in IDD specimens, in H2 O2 -induced cell senescence model in NPCs...
March 9, 2018: Journal of Cellular Biochemistry
Chencheng Feng, Yang Zhang, Minghui Yang, Minghong Lan, Bo Huang, Huan Liu, Yue Zhou
High oxygen tension caused by neovascularization in the microenvironment of intervertebral discs (IVDs) is associated with the pathogenesis of IVD degeneration (IDD). Pre-mRNAs undergo alternative splicing (AS) to produce structurally and functionally diverse mRNA and proteins. However, the precise role of high oxygen tension in IDD and the relationship between AS and high oxygen tension in disc cells remain unknown. To investigate the effect of high oxygen tension on disc cells, Affymetrix Rat Transcriptome Array 1...
February 28, 2018: International Journal of Molecular Medicine
Chencheng Feng, Minghui Yang, Yang Zhang, Minghong Lan, Bo Huang, Huan Liu, Yue Zhou
Intervertebral disc (IVD) degeneration (IDD) is a widely recognized contributor to low back pain. Mechanical stress is a crucial etiological factor of IDD. During the process of IDD, a vicious circle is formed between abnormal mechanical stress and the damage of disc structure and function. Notably, the pathological process of IDD is mediated by the phenotypic shift of IVD cells from an extracellular matrix anabolic phenotype to a catabolic and pro-inflammatory phenotype. Therefore, the effects of mechanical stress on the initiation and progression of IDD depend on the mechanobiology of IVD cells...
February 28, 2018: International Journal of Molecular Medicine
Masayuki Miyagi, Kentaro Uchida, Shotaro Takano, Hisako Fujimaki, Jun Aikawa, Hiroyuki Sekiguchi, Naoshige Nagura, Seiji Ohtori, Gen Inoue, Masashi Takaso
Upregulation of inflammatory cytokines and various growth factors is a significant contributor to discogenic low back pain. The aim of this study was to investigate possible regulation of pain-related molecules by macrophages and the role of macrophage-derived molecules in injured intervertebral disc (IVD)s. C57BL/6J mice were used in this study. We characterized the expression profiles of genes for tumor necrosis factor (TNF)-alpha, interleukin (IL)-1beta, nerve growth factor (NGF), and vascular endothelial growth factor (VEGF) in both intact and injured IVDs...
March 6, 2018: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
Min Ho Hwang, Hyeong Guk Son, Jae Won Lee, Chang Min Yoo, Jae Hee Shin, Hyo Geun Nam, Hyun Jung Lim, Seung Min Baek, Jeong Hun Park, Joo Han Kim, Hyuk Choi
The etiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no curative therapies. Painful IVD degeneration is associated with infiltrated macrophage-mediated inflammatory response of human nucleus pulposus (NP) cells. The present study aimed to address the hypothesis that pro-inflammatory cytokines derived from macrophages lead to the altered molecular phenotype of human NP cells and to investigate the effects of phototherapy (630, 525, 465 nm with 16, 32, 64 J/cm2 ) on pain-related cytokine interleukin (IL)-6 and chemokine IL-8 under inflammatory conditions in human NP cells...
March 3, 2018: Lasers in Medical Science
Marc E Pfeifer
U.S. studies show that the global point-of-care (POC) diagnostics market will reach $40.5 bn by 2022, with a compound annual growth rate (CAGR) of 10%. This is one of the reasons why HES-SO Valais-Wallis and CSEM, acting on behalf of the NTN Swiss Biotech thematic platform in vitro Diagnostics (TP IVD), invited interested parties on October 26, 2017 to the SWISS SYMPOSIUM in Point-of-Care Diagnostics (see CHIMIA No. 12/2017). We now bring the second report on the future prospects of POC diagnostics.
February 1, 2018: Chimia
Mick Perez-Cruet, Naimisha Beeravolu, Christina McKee, Jared Brougham, Irfan Khan, Shreeya Bakshi, G Rasul Chaudhry
BACKGROUND: Degenerative disc disease (DDD) is a common spinal disorder that manifests with neck and lower back pain caused by the degeneration of intervertebral discs (IVDs). Currently, there is no treatment to cure this debilitating ailment. OBJECTIVE: To investigate the potential of nucleus pulposus (NP)-like cells (NPCs) derived from human umbilical cord mesenchymal stem cells (MSCs) to restore degenerated IVDs using a rabbit DDD model. METHODS: NPCs differentiated from MSCs were characterized using quantitative real-time reverse transcription polymerase chain reaction and immunocytochemical analysis...
February 26, 2018: Neurosurgery
Alexander Kolb, Simon Robinson, David Stelzeneder, Markus Schreiner, Catharina Chiari, Reinhard Windhager, Siegfried Trattnig, Klaus Bohndorf
OBJECTIVES: To evaluate the clinical feasibility of ultrahigh field 7-T SWI to visualize vessels and assess their density in the immature epiphyseal cartilage of human knee joints. METHODS: 7-T SWI of 12 knees (six healthy volunteers, six patients with osteochondral abnormalities; mean age 10.7 years; 3 female, 9 male) were analysed by two readers, classifying intracartilaginous vessel densities (IVD) in three grades (no vessels, low IVD and high IVD) in defined femoral, tibial and patellar zones...
February 26, 2018: European Radiology
M C Kriegmair, R M Wirtz, T S Worst, J Breyer, M Ritter, B Keck, C Boehmer, W Otto, M Eckstein, C A Weis, A Hartmann, C Bolenz, P Erben
INTRODUCTION: Gene expression analyses have identified similarities between bladder and breast cancer, where clinical risk stratification is based on Her2, ESR1, PGR and Ki67 expression. The aim of the study was to assess the respective marker gene expression in patients treated with radical cystectomy for muscle-invasive bladder cancer (MIBC) and to evaluate the applicability of breast cancer subtypes for MIBC risk stratification. MATERIALS & METHODS: 102 patients treated with radical cystectomy for MIBC were assessed...
February 22, 2018: Translational Oncology
X Liu, D Krishnamoorthy, L Lin, P Xue, F Zhang, L Chi, R J Linhardt, J C Iatridis
Intervertebral disc (IVD) degeneration results in the depletion of proteoglycans and glycosaminoglycans (GAGs), which can lead to structural and mechanical loss of IVD function, ingrowth of nociceptive nerve fibres and eventually discogenic pain. Specific GAG types as well as their disaccharide patterns can be predictive of disease and degeneration in several tissues but have not been comprehensively studied within the IVD. A highly sensitive mass spectrometry based technique with multiple reaction monitoring (MRM) was used to provide characterisation of chondroitin sulphate (CS), hyaluronic acid (HA), heparan sulphate (HS) and their disaccharide sulphation patterns across different anatomical regions of human IVDs...
February 22, 2018: European Cells & Materials
Susan Wopat, Jennifer Bagwell, Kaelyn D Sumigray, Amy L Dickson, Leonie F A Huitema, Kenneth D Poss, Stefan Schulte-Merker, Michel Bagnat
The spine is a segmented axial structure made of alternating vertebral bodies (centra) and intervertebral discs (IVDs) assembled around the notochord. Here, we show that, prior to centra formation, the outer epithelial cell layer of the zebrafish notochord, the sheath, segments into alternating domains corresponding to the prospective centra and IVD areas. This process occurs sequentially in an anteroposterior direction via the activation of Notch signaling in alternating segments of the sheath, which transition from cartilaginous to mineralizing domains...
February 20, 2018: Cell Reports
Frank A Alexander, Sebastian Eggert, Joachim Wiest
Skin is a critical organ that plays a crucial role in defending the internal organs of the body. For this reason, extensive work has gone into creating artificial models of the epidermis for in vitro skin toxicity tests. These tissue models, called reconstructed human epidermis (RhE), are used by researchers in the pharmaceutical, cosmetic, and environmental arenas to evaluate skin toxicity upon exposure to xenobiotics. Here, we present a label-free solution that leverages the use of the intelligent mobile lab for in vitro diagnostics (IMOLA-IVD), a noninvasive, sensor-based platform, to monitor the transepithelial electrical resistance (TEER) of RhE models and adherent cells cultured on porous membrane inserts...
February 21, 2018: Genes
Shingo Miyazaki, Ashish D Diwan, Kenji Kato, Kevin Cheng, Won C Bae, Yang Sun, Junichi Yamada, Carol Muehleman, Mary E Lenz, Nozomu Inoue, Robert L Sah, Mamoru Kawakami, Koichi Masuda
PURPOSE: To elucidate the effects of growth differentiation factor-6 (GDF6) on: (i) gene expression of inflammatory/pain-related molecules and structural integrity in the rabbit intervertebral disc (IVD) degeneration model, and (ii) sensory dysfunction and changes in pain-marker expression in dorsal nerve ganglia (DRGs) in the rat xenograft radiculopathy model. METHODS: Forty-six adolescent rabbits received anular-puncture in two non-consecutive lumbar IVDs. Four weeks later, phosphate-buffered saline (PBS) or GDF6 (1, 10 or 100 µg) was injected into the nucleus pulposus (NP) of punctured discs and followed for 4 weeks for gene expression analysis and 12 weeks for structural analyses...
February 19, 2018: European Spine Journal
Xing Yang, Lei Wang, Zhang-Qin Yuan, Ping-Hui Zhou, Geng-Lei Chu, Bin Li, Jun-Ying Sun
The loss of intervertebral disc (IVD) cells due to excessive apoptosis is a central factor in the process of IVD degeneration. However, whether interleukin-1β (IL-1β) induces apoptosis through the extracellular signal-regulated kinase (ERK) pathway in annulus fibrosus cells (AFCs) has not been investigated. In this study, we observed increases in expression of Bax, caspase-3, and caspase-9, and a decrease in expression of Bcl-2 in patients with degenerative IVDs. In vitro, we found that IL-1β induced apoptosis and ERK pathway activation in AFCs...
February 18, 2018: Connective Tissue Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"