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https://www.readbyqxmd.com/read/28226537/automated-transepithelial-electrical-resistance-measurements-of-the-epiderm-reconstructed-human-epidermis-model
#1
Frank A Alexander, Joachim Wiest, Frank A Alexander, Joachim Wiest, Joachim Wiest, Frank A Alexander
Understanding the effect of exogenous substances on human skin is critical for toxicology assessment. To address this, numerous artificial models of the topmost layer of human skin, so-called reconstructed human epidermis (RhE), have been created in an attempt to produce a clear analogue for testing. Unfortunately, current testing modalities still rely on endpoint assays and are not capable of monitoring time-resolved changes in barrier function without using numerous redundant samples. In this work, a novel, time-resolved approach is realized by monitoring the transepithelial electrical resistance (TEER) of MatTek EpiDerm® reconstructed human epidermis model, utilizing an automated protocol with the Intelligent Mobile Lab for in vitro diagnostics (IMOLA-IVD)...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28207788/self-complementary-adeno-associated-virus-serotype-6-mediated-knockdown-of-adamts4-induces-long-term-and-effective-enhancement-of-aggrecan-in-degenerative-human-nucleus-pulposus-cells-a-new-therapeutic-approach-for-intervertebral-disc-disorders
#2
Demissew Shenegelegn Mern, Anja Tschugg, Sebastian Hartmann, Claudius Thomé
Inhibition of intervertebral disc (IVD) degeneration, which is often accompanied by painful inflammatory and immunopathological processes, is challenging. Current IVD gene therapeutic approaches are based on adenoviral gene delivery systems, which are limited by immune reactions to their viral proteins. Their applications in IVDs near to sensitive neural structure could provoke toxicity and immunological side-effects with neurological deficits. Self-complementary adeno-associated virus (scAAV) vectors, which do not express any viral gene and are not linked with any known disease in humans, are attractive therapeutic gene delivery vectors in degenerative IVDs...
2017: PloS One
https://www.readbyqxmd.com/read/28207656/biomechanical-evaluation-of-transpedicular-nucleotomy-with-intact-annulus-fibrosus
#3
Fabrizio Russo, Robert A Hartman, Kevin M Bell, Nam Vo, Gwendolyn A Sowa, James D Kang, Gianluca Vadalà, Vincenzo Denaro
STUDY DESIGN: Biomechanical testing of partially nucleotomized ovine cadaveric spines. OBJECTIVE: To explore how the nucleus pulposus (NP) affects the biomechanical behavior of the intervertebral disc (IVD) by performing a partial nucleotomy via the transpedicular approach. SUMMARY OF BACKGROUND DATA: Mechanical loading represents a crucial part of IVD homeostasis. However, traditional regenerative strategies require violation of the annulus fibrosus (AF) resulting in significant alteration of joint mechanics...
February 15, 2017: Spine
https://www.readbyqxmd.com/read/28205452/in-vivo-dosimetry-in-uk-external-beam-radiotherapy-current-and-future-usage
#4
Niall D MacDougall, Michael Graveling, Vibeke N Hansen, Kevin Brownsword, Andrew Morgan
OBJECTIVES: Towards Safer Radiotherapy (1) recommended that radiotherapy centres should have protocols in place for in vivo dosimetry (IVD) monitoring at the beginning of patient treatment courses. This report determines IVD implementation in the UK in 2014, the methods used and makes recommendations on future use. METHODS: Evidence from peer-reviewed journals was used in conjunction with the first survey of UK radiotherapy centres' IVD practice since the publication of TSRT...
February 16, 2017: British Journal of Radiology
https://www.readbyqxmd.com/read/28202026/an-indirect-enzyme-linked-immunosorbent-assay-for-the-identification-of-antibodies-to-senecavirus-a-in-swine
#5
Cheryl M T Dvorak, Zeynep Akkutay-Yoldar, Suzanne R Stone, Steven J P Tousignant, Fabio A Vannucci, Michael P Murtaugh
BACKGROUND: Senecavirus A (SVA), a member of the family Picornaviridae, genus Senecavirus, is a recently identified single-stranded RNA virus closely related to members of the Cardiovirus genus. SVA was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with Idiopathic Vesicular Disease (IVD). Vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal diseases, such as foot-and-mouth disease (FMD), whose presence would be economically devastating to the United States...
February 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28201998/clinical-performance-evaluation-of-a-sensitive-rapid-low-throughput-test-for-kras-mutation-analysis-using-formalin-fixed-paraffin-embedded-tissue-samples
#6
Christine Weyn, Sofie Van Raemdonck, Robina Dendooven, Vincent Maes, Karen Zwaenepoel, Suzan Lambin, Patrick Pauwels
BACKGROUND: Testing for KRAS mutations in metastatic colorectal cancer (mCRC) on formalin-fixed, paraffin embedded (FFPE) tumor tissue has become standard of care. Different molecular methods exist to determine hotspot KRAS mutations in exon 2, 3 and 4, but testing is often limited by the sensitivity and the speed of analysis. The aim of this retrospective study was to establish the clinical performance of the Idylla™ KRAS Mutation Test on FFPE tumor samples of patients with mCRC. METHODS: KRAS mutation analysis was performed using the therascreen KRAS on the RotorGene Q platform (CE-IVD; Qiagen) and results were subsequently compared to the Idylla™ KRAS Mutation Test...
February 16, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28197282/fragmented-qrs-as-a-marker-of-electrical-dyssynchrony-to-predict-inter-ventricular-conduction-defect-by-subsequent-echocardiographic-assessment-in-symptomatic-patients-of-non-ischemic-dilated-cardiomyopathy
#7
Santosh Kumar Sinha, Kush Bhagat, Mohammad Asif, Karandeep Singh, Mohit Sachan, Vikas Mishra, Nasar Afdaali, Mukesh Jitendra Jha, Ashutosh Kumar, Shravan Singh, Rupesh Sinha, Dibbendhu Khanra, Ramesh Thakur, Chandra Mohan Varma, Vinay Krishna, Umeshwar Pandey
BACKGROUND: Left ventricular (LV) dyssynchrony frequently occurs in patients with heart failure (HF). QRS ≥ 120 ms is a surrogate marker of electrical dyssynchrony, which occurs in only 30% of HF patients. In contrary, in those with normal QRS (nQRS) duration, LV dyssynchrony has been reported in 20-50%. This study was carried out to investigate the role of fragmented QRS (fQRS) on the surface electrocardiography (ECG) as a marker of electrical dyssynchrony to predict the presence of significant intraventricular dyssynchrony (IVD) by subsequent echocardiographic assessment...
August 2016: Cardiology Research
https://www.readbyqxmd.com/read/28188410/simvastatin-inhibits-il-1%C3%AE-induced-apoptosis-and-extracellular-matrix-degradation-by-suppressing-the-nf-kb-and-mapk-pathways-in-nucleus-pulposus-cells
#8
Tu Ji, Li Wentian, Zhang Yukun, Wu Xinghuo, Song Yu, Kang Liang, Liu Wei, Wang Kun, Li Shuai, Hua Wenbin, Yang Cao
Statins are widely used hypocholesterolemic drugs that block the mevalonate pathway. Some studies have shown that statins may have the potential to inhibit intervertebral disk (IVD) degeneration (IDD). Interleukin (IL)-1β, a catabolic cytokine, is a key regulator of IDD. This study aimed to investigate the mechanism underlying the effect of simvastatin on IDD. The viability of nucleus pulposus (NP) cells was determined by the methyl-thiazolyl-tetrazolium (MTT) assay. The apoptosis of NP cells was measured by flow cytometric analysis, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL), and western blotting of relevant apoptotic proteins...
February 10, 2017: Inflammation
https://www.readbyqxmd.com/read/28187077/comparison-of-synchrotron-radiation-based-propagation-phase-contrast-imaging-and-conventional-micro-computed-tomography-for-assessing-intervertebral-discs-and-endplates-in-a-murine-model
#9
Jianzhong Hu, Shuangfei Ni, Yong Cao, Xiaokai Wang, Shenghui Liao, Hongbin Lu
STUDY DESIGN: The synchrotron radiation based micro-CT (SRμCT) and micro-CT (μCT) were applied to comparatively assess the intervertebral disc (IVD) and endplate (EP). OBJECTIVE: To explore a new approach to evaluate the detailed structure of the IVD and EP during maturation and aging in a murine model. SUMMARY OF BACKGROUND DATA: To date, methods to observe the morphological changes in the IVD and EP from rodent have been relatively limited...
February 9, 2017: Spine
https://www.readbyqxmd.com/read/28167391/bioengineered-three-dimensional-diseased-intervertebral-disc-model-revealed-inflammatory-crosstalk
#10
Akshay Srivastava, Isma Liza Mohd Isa, Peadar Rooney, Abhay Pandit
Without an appropriate disease model, the understanding of the pathophysiology of intervertebral disc degeneration and inflammation is limited. The lack of understanding limits the potential discovery of therapeutic targets as viable treatment options. Here, we report a versatile method to develop a three-dimensional intervertebral disc (IVD) model to study the response of nucleus pulposus (NP) and annulus fibrosus (AF) cells to inflammatory (IL-1β-induced) stimulation. The cell shape regulated IVD model was engineered by modulating the crosslinking of a self-assembled collagen hydrogel...
January 31, 2017: Biomaterials
https://www.readbyqxmd.com/read/28152249/feasibility-study-for-evaluating-early-lumbar-facet-joint-degeneration-using-axial-t1-%C3%AF-t2-and-t2-mapping-in-cartilage
#11
Jianzhong Hu, Yi Zhang, Chunyue Duan, Xianjing Peng, Ping Hu, Hongbin Lu
PURPOSE: To assess the feasibility of axial T2 , T2*, and T1 ρ mapping of lumbar facet joint (LFJ) cartilage for evaluation of early degeneration. MATERIALS AND METHODS: We examined a total of 176 LFJs from 21 volunteers using axial T2 , T2*, and T1 ρ mapping with a 3.0T magnetic resonance imaging (MRI) scanner. All LFJs were measured and grouped according to the presence of low back pain (LBP), the Weishaupt grading system, and the Pfirrmann grade of the adjacent intervertebral disk (IVD)...
February 2, 2017: Journal of Magnetic Resonance Imaging: JMRI
https://www.readbyqxmd.com/read/28149534/iapp-modulates-cellular-autophagy-apoptosis-and-extracellular-matrix-metabolism-in-human-intervertebral-disc-cells
#12
Xinghuo Wu, Yu Song, Wei Liu, Kun Wang, Yong Gao, Shuai Li, Zhenfeng Duan, Zengwu Shao, Shuhua Yang, Cao Yang
The pathogenic process of intervertebral disc degeneration (IDD) is characterized by imbalance in the extracellular matrix (ECM) metabolism. Nucleus pulposus (NP) cells have important roles in maintaining the proper structure and tissue homeostasis of disc ECM. These cells need adequate supply of glucose and oxygen. Islet amyloid polypeptide (IAPP) exerts its biological effects by regulating glucose metabolism. The purpose of this study was to investigate the expression of IAPP in degenerated IVD tissue, and IAPP modulation of ECM metabolism in human NP cells, especially the crosstalk mechanism between apoptosis and autophagy in these cells...
2017: Cell Death Discovery
https://www.readbyqxmd.com/read/28146021/spinal-tissue-loading-created-by-different-methods-of-spinal-manipulative-therapy-smt-application
#13
Martha Funabashi, François Nougarou, Martin Descarreaux, Narasimha Prasad, Gregory N Kawchuk
STUDY DESIGN: Comparative study using robotic replication of SMT vertebral kinematics together with serial dissection. OBJECTIVE: To quantify loads created in cadaveric spinal tissues arising from three different forms of SMT application. SUMMARY OF BACKGROUND DATA: There exist many distinct methods by which to apply spinal manipulative therapy (SMT). It is not known presently if different forms of SMT application have different effects on spinal tissues...
January 31, 2017: Spine
https://www.readbyqxmd.com/read/28146016/individual-collagen-fibril-thickening-and-stiffening-of-annulus-fibrosus-in-degenerative-intervertebral-disc
#14
Ting Liang, Lin-Lin Zhang, Wei Xia, Hui-Lin Yang, Zong-Ping Luo
STUDY DESIGN: In vitro study using rat intervertebral discs (IVDs). OBJECTIVE: To explore the alteration of annulus fibrosus collagen fibrils after loading on IVD and to investigate the degeneration pathogenesis at the nanoscale. SUMMARY OF BACKGROUND DATA: Abnormal loading can lead to IVD degeneration, but the precise mechanism has been hitherto elusive, especially at the nanoscale. METHODS: A rat IVD loading model was used, which combined bending of the tail by 40° with compressive loading of 1...
January 31, 2017: Spine
https://www.readbyqxmd.com/read/28145804/coculture-with-partial-digestion-notochordal-cell-rich-nucleus-pulposus-tissue-activates-degenerative-human-nucleus-pulposus-cells
#15
Xue-Dong Bai, Xiao-Chuan Li, Jia-Hai Chen, Zi-Ming Guo, Li-Sheng Hou, De-Li Wang, Qing He, Di-Ke Ruan
Recent studies suggested that notochordal cells (NCs) and NC conditioned medium (NCCM) can stimulate cell viability and matrix production of nucleus pulposus cells (NPCs). However, the potential of notochordal cell-rich nucleus pulposus (NRNP) incorporating the native environment of the intervertebral disc has not been evaluated. The objective of this study was to develop an optimal NRNP model and test whether it can allow a significant level of NPC activation in vitro. Rabbit NRNP explants were divided into three groups according to different digestion time: digestion NRNP of 8 hours, partial digestion NRNP of 2 hours, and natural NRNP ...
February 1, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28143419/t2-relaxation-time-for-intervertebral-disc-degeneration-in-patients-with-upper-back-pain-initial-results-on-the-clinical-use-of-3-0-tesla-mri
#16
Raoying Xie, Linhui Ruan, Lei Chen, Kai Zhou, Jiandong Yuan, Wei Ji, Guangjian Jing, Xiaojing Huang, Qinglei Shi, Chun Chen
BACKGROUND: Magnetic resonance imaging (MRI) is a useful non-invasive tool for evaluating abnormalities of intervertebral discs. However, there are few studies which applied functional MRI techniques to investigate degenerative changes in cervical and cervicothoracic junction (CTJ) spine among adults. The aim of this study was to compare T2 relaxation time measurement evaluation with morphological grading for assessing cervical and CTJ intervertebral discs (IVD) in the patients suffering neck, shoulder, and upper back pain...
January 31, 2017: BMC Medical Imaging
https://www.readbyqxmd.com/read/28130756/comparison-of-five-different-assays-for-the-detection-of-braf-mutations-in-formalin-fixed-paraffin-embedded-tissues-of-patients-with-metastatic-melanoma
#17
Claire Franczak, Julia Salleron, Cindy Dubois, Pierre Filhine-Trésarrieu, Agnès Leroux, Jean-Louis Merlin, Alexandre Harlé
BACKGROUND: Metastatic or unresectable melanoma is a serious and deadly disease. Anti-BRAF and immunotherapy improved overall survival in patients with metastatic disease. Thus, BRAF genotyping is important to choose the right therapy. METHODS: In our study, we assessed and compared BRAF mutations in 59 formalin-fixed and paraffin-embedded tumor samples of patients with metastatic melanoma with next-generation sequencing (NGS), Cobas(®) 4800 BRAF V600 mutation test CE-IVD commercial kit, high-resolution melting PCR (HRM), multiplex real-time allele specific amplification (multiplexed RT-ASA) and immunohistochemistry (IHC)...
January 27, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28130014/-biology-and-mechanobiology-of-the-intervertebral-disc
#18
Emilio González Martínez, José García-Cosamalón, Iván Cosamalón-Gan, Marta Esteban Blanco, Olivia García-Suarez, José A Vega
The intervertebral disc (IVD) is noted for its low cell content, and being the largest avascular structure of human body. The low amount of cells in the disc have to adapt to an anaerobic metabolism with low oxygen pressure and acidic pH. Apart from surviving in an adverse microenvironment, they are exposed to a high level of mechanical stress. The biological adaptation of cells to acidosis and hyperosmolarity conditions are regulated by mechanoproteins, which are responsible for converting a mechanical signal into a cellular response, thus modifying its gene expression...
January 24, 2017: Neurocirugía
https://www.readbyqxmd.com/read/28113094/validity-of-the-modified-berg-balance-scale-in-adults-with-intellectual-and-visual-disabilities
#19
Annemarie Dijkhuizen, Wim P Krijnen, Cees P van der Schans, Aly Waninge
BACKGROUND: A modified version of the Berg Balance Scale (mBBS) was developed for individuals with intellectual and visual disabilities (IVD). However, the concurrent and predictive validity has not yet been determined. AIM: The purpose of the current study was to evaluate the concurrent and predictive validity of the mBBS for individuals with IVD. METHOD: Fifty-four individuals with IVD and Gross Motor Functioning Classification System (GMFCS) Levels I and II participated in this study...
January 20, 2017: Research in Developmental Disabilities
https://www.readbyqxmd.com/read/28108404/annulus-fibrosus-cells-express-and-utilize-c-c-chemokine-receptor-5-ccr5-for-migration
#20
Weijun Liu, David Liu, Justin Zheng, Peng Shi, Po-Hsin Chou, Chundo Oh, Di Chen, Howard S An, Ana Chee
BACKGROUND CONTEXT: Disc degeneration is associated with the progressive loss of the proteoglycan content of the intervertebral disc, decreased matrix synthesis, higher concentrations of proteolytic enzymes, and increased levels of proinflammatory cytokines. In previous studies, we have shown that C-C chemokine ligand (CCL)2, CCL3, and CCL5 are highly expressed by cultured nucleus pulposus (NP) and annulus fibrosus (AF) cells that have been treated by interleukin-1. The major function of these chemokines is to recruit immune cells into the disc...
January 17, 2017: Spine Journal: Official Journal of the North American Spine Society
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