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https://www.readbyqxmd.com/read/29784673/formation-of-the-sacrum-requires-down-regulation-of-sonic-hedgehog-signaling-in-the-sacral-intervertebral-discs
#1
Raffaella Bonavita, Kathleen Vincent, Robert Pinelli, Chitra Lekha Dahia
In humans, the sacrum forms an important component of the pelvic arch, and it transfers the weight of the body to the lower limbs. The sacrum is formed by collapse of the intervertebral discs (IVDs) between the five sacral vertebrae during childhood, and their fusion to form a single bone. We show that collapse of the sacral discs in the mouse is associated with the down-regulation of sonic hedgehog (SHH) signaling in the nucleus pulposus (NP) of the disc, and many aspects of this phenotype can be reversed by experimental postnatal activation of HH signaling...
May 21, 2018: Biology Open
https://www.readbyqxmd.com/read/29779014/current-insights-on-use-of-growth-factors-as-therapy-for-intervertebral-disc-degeneration
#2
Justin C Kennon, Mohamed E Awad, Norman Chutkan, John DeVine, Sadanand Fulzele
Chronic low back pain is a critical health problem and a leading cause of disability in aging populations. A major cause of low back pain is considered to be the degeneration of the intervertebral disc (IVD). Recent advances in therapeutics, particularly cell and tissue engineering, offer potential methods for inhibiting or reversing IVD degeneration, which have previously been impossible. The use of growth factors is under serious consideration as a potential therapy to enhance IVD tissue regeneration. We reviewed the role of chosen prototypical growth factors and growth factor combinations that have the capacity to improve IVD restoration...
May 19, 2018: Biomolecular Concepts
https://www.readbyqxmd.com/read/29778758/major-traits-of-the-senescent-phenotype-of-nucleus-pulposus-intervertebral-disc-cells-persist-under-the-specific-microenvironmental-conditions-of-the-tissue
#3
Anastasios Kouroumalis, Eleni Mavrogonatou, Olga D Savvidou, Panayiotis J Papagelopoulos, Harris Pratsinis, Dimitris Kletsas
Intervertebral discs (IVDs) are the joints of the spine, mainly consisting of extracellular matrix (ECM) with a low number of cells embedded therein. Low cellularity stems from nutrient deprivation due to the lack of blood supply, as well as from the hypoxic and hyperosmotic conditions prevailing in the tissue. Intervertebral disc degeneration (IDD) has been firmly connected with low back pain, a major age-related disease, whereas degenerated discs have been characterized by increased proteolytic activity and accumulation of senescent cells...
May 17, 2018: Mechanisms of Ageing and Development
https://www.readbyqxmd.com/read/29761413/comparison-of-b-0-versus-b-0-and-b-1-field-inhomogeneity-correction-for-glycosaminoglycan-chemical-exchange-saturation-transfer-imaging
#4
Anja Müller-Lutz, Alexandra Ljimani, Julia Stabinska, Moritz Zaiss, Johannes Boos, Hans-Jörg Wittsack, Christoph Schleich
PURPOSE: The study compares glycosaminoglycan chemical exchange saturation transfer (gagCEST) imaging of intervertebral discs corrected for solely B0 inhomogeneities or both B0 and B1 inhomogeneities. METHODS: Lumbar intervertebral discs of 20 volunteers were examined with T2 -weighted and gagCEST imaging. Field inhomogeneity correction was performed with B0 correction only and with correction of both B0 and B1 . GagCEST effects measured by the asymmetric magnetization transfer ratio (MTRasym ) and signal-to-noise ratio (SNR) were compared between both methods...
May 14, 2018: Magma
https://www.readbyqxmd.com/read/29760807/quality-control-of-next-generation-sequencing-based-in-vitro-diagnostic-test-for-onco-relevant-mutations-using-multiplex-reference-materials-in-plasma
#5
Donglai Liu, Haiwei Zhou, Dawei Shi, Shu Shen, Yabin Tian, Lin Wang, Jiatao Lou, Rong Cong, Juan Lu, Henghui Zhang, Meiru Zhao, Shida Zhu, Zhisheng Cao, Ruilin Jin, Yin Wang, Xiaoni Zhang, Guohua Yang, Youchun Wang, Chuntao Zhang
Background: Widespread clinical implementation of next-generation sequencing (NGS)-based cancer in vitro diagnostic tests (IVDs) highlighted the urgency to establish reference materials which could provide full control of the process from nucleic acid extraction to test report generation. The formalin-fixed, paraffin-embedded (FFPE) tissue and blood plasma containing circulating tumor deoxyribonucleic acid (ctDNA) were mostly used for clinically detecting onco-relevant mutations. Methods: We respectively developed multiplex FFPE and plasma reference materials covering three clinically onco-relevant mutations within the epidermal growth factor receptor ( EGFR ) gene at serial allelic frequencies...
2018: Journal of Cancer
https://www.readbyqxmd.com/read/29752914/twenty-years-of-european-ivd-regulations-and-its-aimed-traceability-where-are-we
#6
EDITORIAL
Joris R Delanghe, Marc L De Buyzere
No abstract text is available yet for this article.
May 9, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29746847/csa-attenuates-compression-induced-nucleus-pulposus-mesenchymal-stem-cells-apoptosis-via-alleviating-mitochondrial-dysfunction-and-oxidative-stress
#7
Zhiliang Li, Songfeng Chen, Kaige Ma, Xiao Lv, Hui Lin, Binwu Hu, Ruijun He, Zengwu Shao
AIMS: This study aims to investigate the protective effects and potential mechanisms of cyclosporine A (CsA), which efficiently inhibits mitochondrial permeability transition pore (MPTP) opening, on compression-induced apoptosis of human nucleus pulposus mesenchymal stem cells (NP-MSCs). MATERIALS AND METHODS: Human NP-MSCs were subjected to various periods of 1.0 MPa compression. Cell viability was evaluated using cell counting kit-8 (CCK-8) assay. The cellular ultrastructure and ATP level were analyzed via transmission electron microscopy (TEM) and ATP detection kit respectively...
May 7, 2018: Life Sciences
https://www.readbyqxmd.com/read/29745502/-influence-analysis-of-aging-of-spinal-segment-on-the-mechanical-behavior-of-vertebral-cortex
#8
Yongtao Lu, Yue Liu, Chengwei Wu
The risk of vertebral cortical shell fracture increases with aging. However, it remains unclear how aging contributes to cortex fracture at present. The aim of this study is to make understanding of the mechanism of how the spinal aging influences the cortical shell strain. Two finite element (FE) models of spinal segments (mildly and fully aged) were created, and then were compared to the FE models of the healthy spinal segment. The FE models of the aged spinal segments were generated by modifying both the geometry of the intervertebral disc (IVD) and the material properties of the spinal components...
June 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29745371/microrna-194-inhibits-lipopolysaccharide-induced-inflammatory-response-in-nucleus-pulposus-cells-of-the-intervertebral-disc-by-targeting-tnf-receptor-associated-factor-6-traf6
#9
Lili Kong, Mingxiao Sun, Zhaofu Jiang, Liming Li, Bing Lu
BACKGROUND The dramatic increase of intervertebral disc degeneration (IDD) is considered to be a major cause of discogenic low back pain. The current study focused on the regulatory function of microRNA-194 (miR-194) on lipopolysaccharide (LPS)-induced inflammatory response in nucleus pulposus (NP) cells. MATERIAL AND METHODS LPS was used to treat NP cells to induce inflammatory responses. MiRNA and gene expression were detected by quantitative PCR. Proteins and protein expression levels were detected by Western blot and ELISA kit...
May 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29730648/metrological-traceability-and-harmonization-of-medical-tests-a-quantum-leap-forward-is-needed-to-keep-pace-with-globalization-and-stringent-ivd-regulations-in-the-21st-century
#10
Christa Cobbaert, Nico Smit, Philippe Gillery
In our efforts to advance the profession and practice of clinical laboratory medicine, strong coordination and collaboration are needed more than ever before. At the dawn of the 21st century, medical laboratories are facing many unmet clinical needs, a technological revolution promising a plethora of better biomarkers, financial constraints, a growing scarcity of well-trained laboratory technicians and a sharply increasing number of International Organization for Standardization guidelines and new regulations to which medical laboratories should comply in order to guarantee safety and effectiveness of medical test results...
May 7, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29723636/mechanical-loading-of-intervertebral-disc-modulates-microglia-proliferation-activation-and-chemotaxis
#11
Stefania Elena Navone, Marianna Peroglio, Laura Guarnaccia, Matteo Beretta, Sibylle Grad, Moira Paroni, Chiara Cordiglieri, Marco Locatelli, Mauro Pluderi, Paolo Rampini, Rolando Campanella, Mauro Alini, Giovanni Marfia
OBJECTIVE: The aim of the study is to assess the effects of the neuroinflammatory microenvironment of a mechanically-induced degenerating IVD on neuroinflammatory like cells such as microglia, in order to comprehend the role of microglial cells in degenerative disc disease. METHODS: Bovine caudal IVDs were kept in culture in an ex vivo bioreactor under high frequency loading and limited nutrition or in free swelling conditions as control samples. Conditioned media (CM) were collected, analysed for cytokine and neurotrophin content and applied to microglial cells for neuroinflammatory activation assessment...
April 30, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29722046/pharmacogenomic-biomarkers-interpretation-of-information-included-in-united-states-and-japanese-drug-labels
#12
R Shimazawa, M Ikeda
WHAT IS KNOWN AND OBJECTIVES: Many drug labels contain information on pharmacogenomic biomarkers (PGBMs), but the information is not necessarily actionable. Pharmacogenomics Knowledgebase (PharmGKB) aims to clarify the level of action for PGBMs (PGx levels) implied in each label as issued by the US Food and Drug Administration. We wished to evaluate the association between the PGx level for US and Japanese drug labels and the insurance coverage for PGBM testing or approval for in vitro diagnostics (IVDs) in each country...
May 2, 2018: Journal of Clinical Pharmacy and Therapeutics
https://www.readbyqxmd.com/read/29713414/evaluation-of-lumbar-intervertebral-disc-degeneration-using-t1%C3%AF-and-t2-magnetic-resonance-imaging-in-a-rabbit-disc-injury-model
#13
Tetsuhiro Ishikawa, Atsuya Watanabe, Hiroto Kamoda, Masayuki Miyagi, Gen Inoue, Kazuhisa Takahashi, Seiji Ohtori
Study Design: An in vivo histologic and magnetic resonance imaging (MRI) study of lumbar intervertebral disc (IVD) degeneration was conducted. Purpose: To clarify the sensitivity and efficacy of T1ρ/T2 mapping for IVD degeneration, the correlation between T1ρ/T2 mapping and degenerative grades and histological findings in the lumbar IVD were investigated. Overview of Literature: The early signs of IVD degeneration are proteoglycan loss, dehydration, and collagen degradation...
April 2018: Asian Spine Journal
https://www.readbyqxmd.com/read/29705378/intervertebral-disc-regeneration-from-cell-therapy-to-the-development-of-novel-bioinspired-endogenous-repair-strategies
#14
Johann Clouet, Marion Fusellier, Anne Camus, Catherine Le Visage, Jérôme Guicheux
Low back pain (LBP), frequently associated with intervertebral disc (IVD) degeneration, is a major public health concern. LBP is currently managed by pharmacological treatments and, if unsuccessful, by invasive surgical procedures, which do not counteract the degenerative process. Considering that IVD cell depletion is critical in the degenerative process, the supplementation of IVD with reparative cells, associated or not with biomaterials, has been contemplated. Recently, the discovery of reparative stem/progenitor cells in the IVD has led to increased interest in the potential of endogenous repair strategies...
April 26, 2018: Advanced Drug Delivery Reviews
https://www.readbyqxmd.com/read/29682674/a-validation-study-of-a-dedicated-software-for-an-automated-in-vivo-dosimetry-control-in-radiotherapy
#15
A Piermattei, F Greco, M Grusio, S Menna, L Azario, G Stimato, E Placidi, S Teodoli, S Cilla, A Porcelli, L Alberico, A Fidanzio
In vivo dosimetry (IVD) is the last step of a radiotherapy quality control program aimed to ensure that the dose delivered is in agreement with that prescribed. IVD procedures based on single detectors are time-consuming and impossible to use for the modern radiotherapy techniques, based on static or kinetic beams (modulated in intensity fluence); this means that more efficient and practical methods are highly recommended. The practical method SOFTDISO, based on the use of electronic portal image device (EPID), provides two tests (i) the R ratio between the reconstructed and the planned isocenter doses to verify an agreement within 5% and (ii) the γ-analysis of the EPID images, to verify γ% ≥ 90% and γmean ≤ 0...
April 23, 2018: Medical & Biological Engineering & Computing
https://www.readbyqxmd.com/read/29678470/ensuring-suitable-quality-of-clinical-measurements-through-design
#16
Anthony Orzechowski, Victoria Petrides, Richard Scopp
To design and deliver high quality, safe and effective products, manufacturers of in vitro diagnostic (IVD) products follow a structured, traceable process for controlling the uncertainty of results reported from their measurement systems. This process and its results however, are not often shared in detail with those outside of the manufacturing company. The objective of this paper is to facilitate discussion by describing some of the best practices used during the IVD design and development process, highlighting some design challenges manufacturers face, and to offer ideas for how IVD manufacturers and laboratories could work together to drive further improvement to public health...
April 17, 2018: Clinical Biochemistry
https://www.readbyqxmd.com/read/29673061/decellularization-and-characterization-of-a-whole-intervertebral-disc-xenograft-scaffold
#17
Austin Hensley, Jess Rames, Victor Casler, Christopher Rood, Joshua Walters, Christopher Fernandez, Sanjitpal Gill, Jeremy Mercuri
Intervertebral disc (IVD) degeneration is a multi-factor process that results in the physical destruction of the nucleus pulposus (NP) and annulus fibrosus (AF). This compromises IVD function and causes significant disability and economic burden. Strategies to replace the entire composite structure of the IVD are limited and most approaches do not recapitulate the heterogenous biochemical composition, micro-architecture or mechanical properties of the native tissue. Our central hypothesis was that donor IVDs which resemble the size and biochemistry of human lumbar IVDs could be successfully decellularized while retaining the tissue's structure and function with the long-term goal of creating a composite scaffold for tissue engineering the human IVD...
April 19, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29652862/inhibitory-effects-of-human-primary-intervertebral-disc-cells-on-human-primary-osteoblasts-in-a-co-culture-system
#18
Rahel D May, Daniela A Frauchiger, Christoph E Albers, Lorin M Benneker, Sandro Kohl, Benjamin Gantenbein
Spinal fusion is a common surgical procedure to address a range of spinal pathologies, like damaged or degenerated discs. After the removal of the intervertebral disc (IVD), a structural spacer is positioned followed by internal fixation, and fusion of the degenerated segment by natural bone growth. Due to their osteoinductive properties, bone morphogenetic proteins (BMP) are applied to promote spinal fusion. Although spinal fusion is successful in most patients, the rates of non-unions after lumbar spine fusion range from 5% to 35%...
April 13, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29651107/circular-rna-circ-4099-is-induced-by-tnf-%C3%AE-and-regulates-ecm-synthesis-by-blocking-mir-616-5p-inhibition-of-sox9-in-intervertebral-disc-degeneration
#19
Hua Wang, Peiheng He, Hehai Pan, Jun Long, Jianru Wang, Zemin Li, Hui Liu, Weiying Jiang, Zhaomin Zheng
Circular RNAs (circRNAs) play important roles in the initiation and development of different diseases. Here, we detected their role in intervertebral disc (IVD) degeneration. An Arraystar human circular RNA microarray assay was used to detect circRNAs in normal and degenerated human IVD nucleus pulposus (NP) tissues. The role of circ-4099 in IVDD and its mechanism were evaluated by qRT-PCR and gain-of-function/loss-of-function studies. Interaction networks for competing endogenous RNAs (ceRNAs), miRNAs, and miRNA target gene were detected by bioinformatics analysis, RNA immunoprecipitation and luciferase assay...
April 13, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29649568/honokiol-alleviates-the-degeneration-of-intervertebral-disc-via-suppressing-the-activation-of-txnip-nlrp3-inflammasome-signal-pathway
#20
Pan Tang, Jia-Ming Gu, Zi-Ang Xie, Yu Gu, Zhi-Wei Jie, Kang-Mao Huang, Ji-Ying Wang, Shun-Wu Fan, Xue-Sheng Jiang, Zhi-Jun Hu
Intervertebral disc degeneration (IVDD) is a multifactorial disease and responsible for many spine related disorders, causes disability in the workforce and heavy social costs all over the world. Honokiol, a low molecular weight natural product, could penetrate into and distribute in IVDs to achieve therapeutic effect in a rat tail model. Therefore, the present study was undertaken to examine the antiinflammatory, antioxidation and chondroprotective effect of honokiol using nucleus pulposus cells and investigate its mechanisms to provide a new basis for future clinical treatment of IVDD...
April 9, 2018: Free Radical Biology & Medicine
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