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https://www.readbyqxmd.com/read/29341939/the-quest-for-equivalence-of-test-results-the-pilgrimage-of-the-dutch-calibration-2-000-program-for-metrological-traceability
#1
REVIEW
Rob T P Jansen, Christa M Cobbaert, Cas Weykamp, Marc Thelen
Calibration 2.000 was initiated 20 years ago for standardization and harmonization of medical tests. The program also intended to evaluate adequate implementation of the In Vitro Diagnostics (IVD) 98/79/EC directive, in order to ensure that medical tests are fit-for-clinical purpose. The Calibration 2.000 initiative led to ongoing verification of test standardization and harmonization in the Netherlands using commutable external quality assessment (EQA)-tools and a type 1 EQA-design, where feasible. National support was guaranteed by involving all laboratory professionals as well as laboratory technicians responsible for EQA and quality officers...
January 17, 2018: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/29323102/propionibacterium-acnes-induces-intervertebral-disc-degeneration-by-promoting-nucleus-pulposus-cell-apoptosis-via-the-tlr2-jnk-mitochondrial-mediated-pathway
#2
Yazhou Lin, Yucheng Jiao, Ye Yuan, Zezhu Zhou, Yuehuan Zheng, Jiaqi Xiao, Changwei Li, Zhe Chen, Peng Cao
Evidence suggests that intervertebral disc degeneration (IVDD) can be induced by Propionibacterium acnes (P. acnes), although the underlying mechanisms are unclear. In this study, we analyzed the pathological changes in degenerated human intervertebral discs (IVDs) infected with P. acnes. Compared with P. acnes-negative samples, P. acnes-positive IVDs showed increased apoptosis of nucleus pulposus cells (NPCs) concomitant with severe IVDD. Then, a P. acnes-inoculated IVD animal model was established, and severe IVDD was induced by P...
January 10, 2018: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/29320615/an-intervertebral-disc-whole-organ-culture-system-to-investigate-proinflammatory-and-degenerative-disc-disease-condition
#3
Gernot Lang, Yishan Liu, Janna Geries, Zhiyu Zhou, David Kubosch, Norbert Südkamp, R Geoff Richards, Mauro Alini, Sibylle Grad, Zhen Li
OBJECTIVE: The aim of this study was to compare the effect of different disease initiators of degenerative disc disease (DDD) within an intervertebral disc (IVD) organ culture system, and to understand the interplay between inflammation and degeneration in the early stage of DDD. DESIGN: Bovine caudal IVDs were cultured within a bioreactor for up to 11 days. Control group was cultured under physiological loading (0.02-0.2 MPa; 0.2 Hz; 2h/day) and high glucose (4...
January 10, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29317264/the-genetics-of-chronic-itch-gene-expression-in-the-skin-of-atopic-dermatitis-and-psoriasis-patients-with-severe-itch
#4
Leigh A Nattkemper, Hong Liang Tey, Rodrigo Valdes-Rodriguez, Helen Lee, Nicholas K Mollanazar, Christian Albornoz, Kristen M Sanders, Gil Yosipovitch
To identify itch-related mediators and receptors that are differentially expressed in pruritic skin, we used RNA sequencing to analyze the complete transcriptome in skin from paired itchy, lesional and non-itchy, non-lesional skin biopsies from 25 atopic dermatitis (AD) and 25 psoriasis patients and site-matched biopsies from 30 healthy controls. This analysis identified 18,000 differentially expressed genes common between itchy atopic and psoriatic skin compared to healthy skin. Of those, almost 2,000 genes were differentially expressed between itchy and non-itchy skin in atopic and psoriatic subjects...
January 6, 2018: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/29312639/progranulin-derived-engineered-protein-atsttrin-suppresses-tnf-%C3%AE-mediated-inflammation-in-intervertebral-disc-degenerative-disease
#5
Hong Ding, Jianlu Wei, Yunpeng Zhao, Yi Liu, Lian Liu, Lei Cheng
Atsttrin, an engineered molecule composed of three fragments of progranulin(PGRN), exerts comparable anti-inflammation ability. Intervertebral disc degeneration (IDD) is involved in inflammation in which TNF-α plays a key role. This study aims to examine the effect and the mechanism of Atsttrin in the pathogenesis of intervertebral disc degeneration. For this purpose, we took advantage of murine and human intervertebral disc (IVD) and examined the expression of TNF-α in IVD tissues using immunohistochemistry and TNF-α level in peripheral sera by ELISA assay...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29310504/the-effect-of-electromagnetic-fields-on-postoperative-pain-and-locomotor-recovery-in-dogs-with-acute-severe-thoracolumbar-intervertebral-disc-extrusion-a-randomized-placebo-controlled-prospective-clinical-trial
#6
Natalia Zidan, Joe Fenn, Emily Griffith, Peter J Early, Chris L Mariani, Karen R Munana, Julien Guevar, Natasha Olby
Spinal cord injury (SCI) due to acute intervertebral disc extrusions (IVDE) is common in dogs and is treated by surgical decompression. Dogs with sensorimotor complete injuries have an incomplete recovery. Pulsed electromagnetic fields (PEMF) reduce postoperative pain through anti-inflammatory effects and there is growing evidence for neuroprotective effects. This randomized, controlled clinical trial evaluated the effect of PEMF on post-operative pain and neurologic recovery in dogs with surgically treated sensorimotor complete SCI due to acute IVDE...
January 8, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29285559/pressure-induced-end-plate-fracture-in-the-porcine-spine-is-the-annulus-fibrosus-susceptible-to-damage
#7
Chelsea R Snow, Maxine Harvey-Burgess, Brigitte Laird, Stephen H M Brown, Diane E Gregory
PURPOSE: To determine if the mechanical properties of the annulus fibrosus (AF) are altered following end-plate fracture. Vertebral fractures, particularly those in the growth plate, are relatively common among adolescents. What is unclear is whether or not these fractures are also associated with concomitant damage to the intervertebral disc (IVD), in particular the AF. METHODS: The current study employed a high-rate IVD pressurization model to create growth plate fractures in the porcine cervical spine...
December 28, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29274053/pregnancy-rate-in-water-buffalo-following-fixed-time-artificial-insemination-using-new-or-used-intravaginal-devices-with-two-progesterone-concentrations
#8
J C Gutiérrez-Añez, R A Palomares, J R Jiménez-Pineda, A R Camacho, G E Portillo-Martínez
This study evaluated the pregnancy rate (PR) after timed artificial insemination (TAI) in water buffalo (Bubalus bubalis) during both non-breeding and breeding season, using either a new or reused intravaginal device (IVD) with two different progesterone concentrations. A total of 247 dairy buffalo cows were randomly assigned using a two-by-three factorial design and four replicates to the following groups: (1) new intravaginal device (IVD-New: DIB®, 1.0 g of P4, n = 51 or CIDR®, 1.38 g of P4, n = 55); (2) intravaginal device previously used once (9 days) (IVD-Used1x: DIB, n = 40 or CIDR, n = 51); or (3) intravaginal device previously used twice (18 days) (IVD-Used2x: DIB, n = 27 or CIDR, n = 23)...
December 22, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/29250209/spine-on-a-chip-human-annulus-fibrosus-degeneration-model-for-simulating-the-severity-of-intervertebral-disc%C3%A2-degeneration
#9
Min Ho Hwang, Dong Hyun Cho, Seung Min Baek, Jae Won Lee, Jeong Hun Park, Chang Min Yoo, Jae Hee Shin, Hyo Geun Nam, Hyeong Guk Son, Hyun Jung Lim, Han Sang Cho, Hong Joo Moon, Joo Han Kim, Jong Kwang Lee, Hyuk Choi
The aetiology of intervertebral disc (IVD) degeneration accompanied by low back pain (LBP) is largely unknown, and there are no effective fundamental therapies. Symptomatic IVD is known to be associated with nerve root compression. However, even in the absence of nerve compression, LBP occurs in patients with IVD degeneration. We hypothesize that this phenomenon is associated with a concentration of pro-inflammatory cytokines such as interleukin (IL)-1β and tumour necrosis factor-alpha (TNF-α), which can lead to altered histologic features and cellular phenotypes observed during IVD degeneration...
November 2017: Biomicrofluidics
https://www.readbyqxmd.com/read/29241405/cell-clusters-are-indicative-of-stem-cell-activity-in-the-degenerate-intervertebral-disc-can-their-properties-be-manipulated-to-improve-intrinsic-repair-of-the-disc
#10
Sharon Brown, Ajay Matta, William Mark Erwin, Sally Roberts, Helen H Gruber, Edward N Hanley, Christopher B Little, James Melrose
The aim of this study was to examine the complexity of the stem cell populations in the intervertebral disc (IVD) and understand their role in disc degeneration with a view to determining whether the resident stem cells could be developed for therapeutic purposes to combat IVD degeneration. Stem cells have been isolated from disc and paradiscal tissues including the notochord, annulus fibrosus (AF), nucleus pulposus (NP), cartilaginous endplate (CEP), ligamentum flavum and vertebral body. Resident AF and NP cells are relatively sparsely distributed occuring as single or occasional doublet cells surrounded by an extensive extracellular matrix (ECM)...
December 14, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/29208967/a-selective-inhibition-of-c-fos-activator-protein-1-as-a-potential-therapeutic-target-for-intervertebral-disc-degeneration-and-associated-pain
#11
Hiroto Makino, Shoji Seki, Yasuhito Yahara, Shunichi Shiozawa, Yukihiko Aikawa, Hiraku Motomura, Makiko Nogami, Kenta Watanabe, Takeshi Sainoh, Hisakatsu Ito, Noriyuki Tsumaki, Yoshiharu Kawaguchi, Mitsuaki Yamazaki, Tomoatsu Kimura
Intervertebral disc (IVD) degeneration is a major cause of low back pain. The transcription factor c-Fos/Activator Protein-1 (AP-1) controls the expression of inflammatory cytokines and matrix metalloproteinases (MMPs) that contribute to the pathogenesis IVD degeneration. We investigated the effects of inhibition of c-Fos/AP-1 on IVD degeneration and associated pain. A selective inhibitor, T-5224, significantly suppressed the interleukin-1β-induced up-regulation of Mmp-3, Mmp-13 and Adamts-5 transcription in human nucleus pulposus cells and in a mouse explant culture model of IVD degeneration...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29207021/stromal-cell%C3%A2-derived-factor%C3%A2-1-induces-matrix-metalloproteinase-expression-in-human-endplate-chondrocytes-cartilage-endplate-degradation-in-explant-culture-and-the-amelioration-of-nucleus-pulposus-degeneration-in-vivo
#12
Hua Zhang, Ting Zhu, Li Zhang, Qionghua Wu
Intervertebral disc (IVD) degeneration is a strong etiological factor in chronic lower back pain. Stem cell migration toward the site of IVD degeneration for regeneration is restricted by avascularity and distance. Our previous study indicated that the expression of stromal cell‑derived factor‑1 (SDF‑1) and its receptor, C-X-C chemokine receptor type 4 (CXCR4) was upregulated in degenerated cartilage endplate (CEP) and nucleus pulposus (NP). In the present study, SDF‑1 increased CXCR4 mRNA and protein expression in human endplate chondrocytes in a dose‑dependent manner...
November 22, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29204735/inflammaging-in-cervical-and-lumbar-degenerated-intervertebral-discs-analysis-of-proinflammatory-cytokine-and-trp-channel-expression
#13
Aleksandra Sadowska, Ermioni Touli, Wolfgang Hitzl, Helen Greutert, Stephen J Ferguson, Karin Wuertz-Kozak, Oliver N Hausmann
PURPOSE: To investigate and compare the occurrence of inflammatory processes in the sites of disc degeneration in the lumbar and cervical spine by a gene array and subsequent qPCR and to investigate the mechanistic involvement of transient receptor potential channels TRPC6 and TRPV4. METHODS: The gene expression of inflammatory cytokines and TRP channels was measured in human disc samples obtained from patients undergoing discectomy at the cervical (n = 24) or lumbar (n = 27) spine for degenerative disc disease (DDD) and disc herniation (DH) and analyzed for differences with regard to spinal level, IVD degeneration grade, Modic grade, age, sex, disc region and surgical extent...
December 4, 2017: European Spine Journal
https://www.readbyqxmd.com/read/29199133/innovative-strategies-for-intervertebral-disc-regenerative-medicine-from-cell-therapies-to-multiscale-delivery-systems
#14
REVIEW
Nina Henry, Johann Clouet, Jean Le Bideau, Catherine Le Visage, Jérôme Guicheux
As our understanding of the physiopathology of intervertebral disc (IVD) degeneration has improved, novel therapeutic strategies have emerged, based on the local injection of cells, bioactive molecules, and nucleic acids. However, with regard to the harsh environment constituted by degenerated IVDs, protecting biologics from in situ degradation while allowing their long-term delivery is a major challenge. Yet, the design of the optimal approach for IVD regeneration is still under debate and only a few papers provide a critical assessment of IVD-specific carriers for local and sustained delivery of biologics...
December 1, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/29195987/physiological-comparability-of-the-harmonized-infogest-in-vitro-digestion-method-to-in-vivo-pig-digestion
#15
Lotti Egger, Patrick Schlegel, Christian Baumann, Helena Stoffers, Dominik Guggisberg, Cédric Brügger, Desirée Dürr, Peter Stoll, Guy Vergères, Reto Portmann
Recently, a static in vitro digestion (IVD) protocol was published by Minekus and coworkers (Minekus et al., 2014) within the COST INFOGEST network. The protocol, concentrating on physiological enzyme activities had the main goal to improve the comparability of experimental data between labs. The protocol was validated in several inter-laboratory studies using skim milk powder (SMP) and indeed demonstrated improved harmonization compared with previous experiments with individual IVD protocols (Egger et al., 2016)...
December 2017: Food Research International
https://www.readbyqxmd.com/read/29194770/differences-in-epidural-pathology-between-cervical-and-thoracolumbar-intervertebral-disk-extrusions-in-dogs
#16
L Züger, A Fadda, A Oevermann, F Forterre, M Vandevelde, D Henke
BACKGROUND: Although the basic pathophysiology is the same in both cervical and thoracolumbar intervertebral disk (IVD) extrusions, there are considerable clinical differences that have only been partially explained. HYPOTHESIS/OBJECTIVES: The epidural inflammatory response differs between cervical and thoracolumbar IVD extrusions. ANIMALS: Fifty-five dogs with cervical and 80 dogs with thoracolumbar IVD extrusions. METHODS: Clinical data and histopathologic variables were investigated...
November 30, 2017: Journal of Veterinary Internal Medicine
https://www.readbyqxmd.com/read/29190883/ghrelin-protects-against-nucleus-pulposus-degeneration-through-inhibition-of-nf-%C3%AE%C2%BAb-signaling-pathway-and-activation-of-akt-signaling-pathway
#17
Weiwei Li, Xihai Wu, Ruize Qu, Wenhan Wang, Xiaomin Chen, Lei Cheng, Yaoge Liu, Linlin Guo, Yunpeng Zhao, Chao Liu
The objective of the present study was to examine the potential role of ghrelin in degeneration of nucleus pulposus (NP). Lower expression levels of ghrelin were found in human NP cells stimulated with interleukin-1β (IL-1β). Moreover, exogenous ghrelin suppressed IL-1β induced degeneration and inflammation associated biomarkers in human NP cells, including matrix metalloproteinase-13, a disintegrin and metalloproteinase with thrombospondin motifs-5, tumor necrosis factor-α and iNOS, which was possibly mediated by antagonization of NF-κB signaling...
November 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/29189572/immunomodulation-of-human-mesenchymal-stem-stromal-cells-in-intervertebral-disc-degeneration-insights-from-a-proinflammatory-degenerative-ex-vivo-model
#18
Graciosa Q Teixeira, Catarina Leite Pereira, Joana R Ferreira, André F Maia, Maria Gomez-Lazaro, Mário A Barbosa, Cornelia Neidlinger-Wilke, Raquel M Goncalves
STUDY DESIGN: Ex vivo experimental study. OBJECTIVE: To investigate the effect of proinflammatory/degenerative intervertebral disc (IVD) microenvironment on the regenerative and immunomodulatory behavior of mesenchymal stem/stromal cells (MSCs), using an ex vivo model from bovine origin. SUMMARY OF BACKGROUND DATA: Low back pain (LBP) is a cause of disability worldwide, most frequently associated with IVD degeneration and inflammation, and characterized by increased levels of inflammatory mediators, often disregarded...
November 17, 2017: Spine
https://www.readbyqxmd.com/read/29185242/costs-and-benefits-of-diagnostic-testing-four-ways-to-improve-patient-care-by-purposive-use-of-in-vitro-diagnostics
#19
Carmen Binder, Maximilian Schmid, Thomas Dieterle, Hans Hendrik Schäfer
Scientific advances and innovative targeted drugs, especially biologics, have revolutionised the treatment of many diseases. In oncology in particular, previously acute or lethal conditions have come to be considered chronic as new treatments have led to longer life expectancies and a lower rate of years lived with disability. These advances, however, come with rising costs in a resource-constrained environment. To achieve cost containment, reimbursement for in-vitro diagnostics (IVDs) is increasingly coming under pressure because they are perceived as a cost factor rather than as a tool to reduce expenditure in the long term...
November 29, 2017: Swiss Medical Weekly
https://www.readbyqxmd.com/read/29181144/evaluation-of-electrospun-nanofiber-anchored-silicone-for-the-degenerative-intervertebral-disc
#20
M Khandaker, S Riahanizad
The nucleus pulposus (NP) substitution by polymeric gel is one of the promising techniques for the repair of the degenerative intervertebral disc (IVD). Silicone gel is one of the potential candidates for a NP replacement material. Electrospun fiber anchorage to silicone disc, referred as ENAS disc, may not only improve the biomechanical performances of the gel but it can also improve restoration capability of the gel, which is unknown. This study successfully produced a novel process to anchor any size and shape of NP gel with electrospun fiber mesh...
2017: Journal of Healthcare Engineering
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