Changsheng He, Cailing Lin, Guosheng Mo, Binbin Xi, An An Li, Dongchao Huang, Yanbin Wan, Feng Chen, Yufeng Liang, Qingxia Zuo, Wanqing Xu, Dongyan Feng, Guanting Zhang, Liya Han, Changwen Ke, Hongli Du, Lizhen Huang
The increasing prevalence of SARS-CoV-2 variants with spike mutations has raised concerns owing to higher transmission rates, disease severity, and escape from neutralizing antibodies. Rapid and accurate detection of SARS-CoV-2 variants provides crucial information concerning the outbreaks of SARS-CoV-2 variants and possible lines of transmission. This information is vital for infection prevention and control. We used a Cas12a-based RT-PCR combined with CRISPR on-site rapid detection system (RT-CORDS) platform to detect the key mutations in SARS-CoV-2 variants, such as 69/70 deletion, N501Y, and D614G...
December 2, 2021: Biosensors & Bioelectronics