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https://www.readbyqxmd.com/read/27847705/a-model-of-glial-scarring-analogous-to-the-environment-of-a-traumatically-injured-spinal-cord-using-kainate
#1
Jong Yoon Yoo, Chang Ho Hwang, Hea Nam Hong
OBJECTIVE: To develop an in vitro model analogous to the environment of traumatic spinal cord injury (SCI), the authors evaluated change of astrogliosis following treatments with kainate and/or scratch, and degree of neurite outgrowth after treatment with a kainate inhibitor. METHODS: Astrocytes were obtained from the rat spinal cord. Then, 99% of the cells were confirmed to be GFAP-positive astrocytes. For chemical injury, the cells were treated with kainate at different concentrations (10, 50 or 100 µM)...
October 2016: Annals of Rehabilitation Medicine
https://www.readbyqxmd.com/read/27790440/outstanding-prevalence-of-methicillin-resistant-staphylococcus-aureus-in-neonatal-omphalitis
#2
Mallika Sengupta, Sayantan Banerjee, Pritam Banerjee, Partha Guchhait
INTRODUCTION: Omphalitis is the infection of the umbilical cord stump, which can lead to septicaemia and significant neonatal morbidity and mortality. Very little data is available on the aetiology of neonatal omphalitis in India. AIM: To identify the causative agents of omphalitis in neonates and determine the antimicrobial susceptibility patterns of the isolates. MATERIALS AND METHODS: A prospective study was conducted at ESI-PGIMSR and ESIC Medical College, Joka, a tertiary care teaching hospital in Eastern India for a period of four months (from 1st January 2016 to 30th April 2016)...
September 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27615153/minimally-invasive-approach-for-the-treatment-of-lumbar-epidural-arteriovenous-fistulas-with-intradural-venous-reflux
#3
A Najjar, F Zairi, T Sunna, A Weil, L Estrade, A Weill, D Shedid
BACKGROUND: Vascular lesions of the spinal cord are increasingly recognized. The most common types of these lesions are dural arteriovenous fistulas (AVFs) whereas, extradural AVFs are a very rare type of spinal AVF and can be associated with either extradural or intradural venous reflux. This results in neurological deficits through congestive or compressive myeloradiculopathy. These lesions must be treated to allow stabilization or improvement of neurologic status, either by endovascular therapy or microsurgical interruption...
September 8, 2016: Neuro-Chirurgie
https://www.readbyqxmd.com/read/27557835/-my-body-was-my-temple-a-narrative-revealing-body-image-experiences-following-treatment-of-a-spinal-cord-injury
#4
K Alysse Bailey, Kimberley L Gammage, Cathy van Ingen, David S Ditor
PURPOSE: This narrative explores the lived experience of a young woman, Rebecca, and her transitioned body image after sustaining and being treated for a spinal cord injury. METHOD: Data were collected from a single semi-structured in-depth interview. RESULTS: Rebecca disclosed her transitioned body image experiences after sustaining a spinal cord injury and being treated by medical staff immediately following her injury. Before her injury, she described a holistic body experience and named this experience her "temple"...
August 24, 2016: Disability and Rehabilitation
https://www.readbyqxmd.com/read/27493991/microfluidic-device-for-the-formation-of-optically-excitable-three-dimensional-compartmentalized-motor-units
#5
Sebastien G M Uzel, Randall J Platt, Vidya Subramanian, Taylor M Pearl, Christopher J Rowlands, Vincent Chan, Laurie A Boyer, Peter T C So, Roger D Kamm
Motor units are the fundamental elements responsible for muscle movement. They are formed by lower motor neurons and their muscle targets, synapsed via neuromuscular junctions (NMJs). The loss of NMJs in neurodegenerative disorders (such as amyotrophic lateral sclerosis or spinal muscle atrophy) or as a result of traumatic injuries affects millions of lives each year. Developing in vitro assays that closely recapitulate the physiology of neuromuscular tissues is crucial to understand the formation and maturation of NMJs, as well as to help unravel the mechanisms leading to their degeneration and repair...
August 2016: Science Advances
https://www.readbyqxmd.com/read/27013456/primary-aspergillosis-of-vocal-cord-long-term-inhalational-steroid-use-can-be-the-miscreant
#6
Arpita Saha, Kaushik Saha, Uttara Chatterjee
Primary laryngeal aspergillosis is extremely rare, especially in an immunocompetent host. It is commonly found as a part of systemic infection in immunocompromised patients. A case of vocal cord aspergillosis with no systemic extension in an immunocompetent patient on long-term steroid metered dose inhaler (MDI) is presented here, because of its rarity. The present case is a 28-year-old asthmatic female who was on inhalational steroid for 8 years, presented with sudden onset of severe dysphonia for 5 days. Fiberoptic laryngoscopy demonstrated whitish plaque involving right vocal cord, clinically suggestive of fungal laryngitis...
December 2015: Biomedical Journal
https://www.readbyqxmd.com/read/26955494/primary-aspergillosis-of-the-larynx
#7
Richard H Law, Samuel A Reyes
Laryngeal aspergillosis is most commonly seen as a result of secondary invasion from the lungs and tracheobronchial tree in immunocompromised hosts. Primary aspergillosis of the larynx is, however, rare with few cases documented over the past fifty years. We report a case of a 73-year-old woman who presented with persistent hoarseness. She is a nonsmoker with a history of asthma and chronic bronchiectasis treated with bronchodilators, inhaled and oral corticosteroids, and nebulized tobramycin. Direct laryngoscopy with vocal cord stripping confirmed the diagnosis of invasive aspergillosis with no manifestations elsewhere...
2016: Case Reports in Otolaryngology
https://www.readbyqxmd.com/read/26850771/-analysis-on-23-cases-of-spinal-meningeal-tuberculosis-by-magnetic-resonance-imaging
#8
Dongpo Wang, Yan Lyu, Xinhua Zhou, Wei He, Pingxin Lyu
OBJECTIVE: To explore the imaging features of spinal cord and spinal meningeal tuberculosis by magnetic resonance (MR). METHODS: We analyzed retrospectively the features of MRI in 23 patients with spinal cord and spinal meningeal tuberculosis compared to 25 patients with spinal cord meningeal metastatic carcinoma. All patients were admitted in our hospital from Jun. 2011 to Oct. 2014. There were 12 males and 11 females with a median age of 26 (range 16-50) years in the spinal cord meningeal tuberculosis group, and 18 males and 7 females with a median age 56 (range 44-78) years in the spinal cord meningeal metastatic carcinoma group...
November 2015: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/26610818/presynaptic-and-postsynaptic-regulation-of-muscle-contractions-in-the-ascarid-nematode-ascaris-suum-a-target-for-drug-action
#9
S M Trailović, Z Zurovac, S Gruborović, D S Marjanović, J Nedeljković-Trailović
The aim of this study was to determine the role in contractions of postsynaptic nicotinic acetylcholine (nACh) and γ-aminobutyric acid (GABA) receptors, in the bag region of Ascaris suum muscle cells, as well as the role of synaptic receptors between interneurons and motor neurons in the dorsal and ventral nerve cord. We have measured the isometric contractions of isolated segments of A. suum, with or without the nerve cord (dorsal or ventral). Contractions were caused by increasing concentrations of ACh or by electrical field stimulation (EFS)...
November 2016: Journal of Helminthology
https://www.readbyqxmd.com/read/26512991/microbial-contaminants-of-cord-blood-units-identified-by-16s-rrna-sequencing-and-by-api-test-system-and-antibiotic-sensitivity-profiling
#10
Luís França, Catarina Simões, Marco Taborda, Catarina Diogo, Milton S da Costa
Over a period of ten months a total of 5618 cord blood units (CBU) were screened for microbial contamination under routine conditions. The antibiotic resistance profile for all isolates was also examined using ATB strips. The detection rate for culture positive units was 7.5%, corresponding to 422 samples.16S rRNA sequence analysis and identification with API test system were used to identify the culturable aerobic, microaerophilic and anaerobic bacteria from CBUs. From these samples we recovered 485 isolates (84 operational taxonomic units, OTUs) assigned to the classes Bacteroidia, Actinobacteria, Clostridia, Bacilli, Betaproteobacteria and primarily to the Gammaproteobacteria...
2015: PloS One
https://www.readbyqxmd.com/read/26471133/multi-electrode-array-for-transcutaneous-lumbar-posterior-root-stimulation
#11
Matthias Krenn, Ursula S Hofstoetter, Simon M Danner, Karen Minassian, Winfried Mayr
Interest in transcutaneous electrical stimulation of the lumbosacral spinal cord is increasing in human electrophysiological and clinical studies. The stimulation effects on lower limb muscles depend on the depolarization of segmentally organized posterior root afferents and, thus, the rostro-caudal stimulation site. In previous studies, selective stimulation was achieved by varying the positions of single self-adhesive electrodes over the thoracolumbar spine. Here, we developed a multi-electrode surface array consisting of 3 × 8 electrode pads and tested its stimulation-site specificity...
October 2015: Artificial Organs
https://www.readbyqxmd.com/read/26200560/distal-spermatic-cord-peritoneal-stripping-an-adjunct-technique-during-inguinal-orchiopexy
#12
Hubert S Swana, Rikin Patel, Mark A Rich
No abstract text is available yet for this article.
May 2015: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/26191208/immunosuppressive-effects-of-mesenchymal-stem-cell-transplantation-in-rat-burn-models
#13
Jinjin Zhang, Xiaolin La, Lixing Fan, Peng Li, Yuanlun Yu, Yongli Huang, Jianbing Ding, Yanchao Xing
OBJECTIVE: This study is to investigate the effects of mesenchymal stem cell (MSC) transplantation in burn treatment. METHODS: Wharton's Jelly was stripped from neonatal umbilical cord, and human umbilical cord MSCs were then cultured. Burn models were constructed in male SD rats weighted at 200±5 g, and the rats were randomly divided into control and MSCs transplantation groups. The rats in transplantation group were injected subcutaneously with MSCs (2×10(6)) at 24 h after burning...
2015: International Journal of Clinical and Experimental Pathology
https://www.readbyqxmd.com/read/26122803/umbilical-cord-milking-versus-delayed-cord-clamping-in-preterm-infants
#14
RANDOMIZED CONTROLLED TRIAL
Anup C Katheria, Giang Truong, Larry Cousins, Bryan Oshiro, Neil N Finer
BACKGROUND AND OBJECTIVE: Delayed cord clamping (DCC) is recommended for premature infants to improve blood volume. Most preterm infants are born by cesarean delivery (CD), and placental transfusion may be less effective than in vaginal delivery (VD). We sought to determine whether infants <32 weeks born by CD who undergo umbilical cord milking (UCM) have higher measures of systemic blood flow than infants who undergo DCC. METHODS: This was a 2-center trial. Infants delivered by CD were randomly assigned to undergo UCM or DCC...
July 2015: Pediatrics
https://www.readbyqxmd.com/read/25878277/non-aggregating-tau-phosphorylation-by-cyclin-dependent-kinase-5-contributes-to-motor-neuron-degeneration-in-spinal-muscular-atrophy
#15
Nimrod Miller, Zhihua Feng, Brittany M Edens, Ben Yang, Han Shi, Christie C Sze, Benjamin Taige Hong, Susan C Su, Jorge A Cantu, Jacek Topczewski, Thomas O Crawford, Chien-Ping Ko, Charlotte J Sumner, Long Ma, Yong-Chao Ma
Mechanisms underlying motor neuron degeneration in spinal muscular atrophy (SMA), the leading inherited cause of infant mortality, remain largely unknown. Many studies have established the importance of hyperphosphorylation of the microtubule-associated protein tau in various neurodegenerative disorders, including Alzheimer's and Parkinson's diseases. However, tau phosphorylation in SMA pathogenesis has yet to be investigated. Here we show that tau phosphorylation on serine 202 (S202) and threonine 205 (T205) is increased significantly in SMA motor neurons using two SMA mouse models and human SMA patient spinal cord samples...
April 15, 2015: Journal of Neuroscience: the Official Journal of the Society for Neuroscience
https://www.readbyqxmd.com/read/25572034/inhibition-of-nmdar-reduces-bladder-hypertrophy-and-improves-bladder-function-in-cyclophosphamide-induced-cystitis
#16
Miao Liu, Shanwei Shen, Derek M Kendig, Sunila Mahavadi, Karnam S Murthy, John R Grider, Li-Ya Qiao
PURPOSE: We examined the role of NMDAR in the regulation of bladder hypertrophy and function in a rat model of cyclophosphamide induced cystitis. MATERIALS AND METHODS: Cystitis was induced by intraperitoneal injection of cyclophosphamide (150 mg/kg body weight). NMDAR phosphorylation (activity) and signal transduction pathways were examined by direct measurement and by specific inhibitors in vivo. Bladder hypertrophy was measured by bladder weight/body weight and type I collagen expression...
May 2015: Journal of Urology
https://www.readbyqxmd.com/read/25526873/delayed-cord-clamping-with-and-without-cord-stripping-a-prospective-randomized-trial-of-preterm-neonates
#17
RANDOMIZED CONTROLLED TRIAL
Margaret S Krueger, Fabien G Eyal, Keith J Peevy, Charles R Hamm, Richard M Whitehurst, David F Lewis
OBJECTIVE: Autologous blood transfusion from the placenta to the neonate at birth has been proven beneficial. Transfusion can be accomplished by either delayed cord clamping or cord stripping. Both are equally effective in previous randomized trials. We hypothesized that combining these 2 techniques would further improve outcomes in preterm neonates. STUDY DESIGN: This was a prospective randomized trial for singleton deliveries with estimated gestational ages between 22 and 31 6/7 weeks...
March 2015: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/25445484/synaptic-rearrangement-following-axonal-injury-old-and-new-players
#18
REVIEW
Aline Barroso Spejo, Alexandre L R Oliveira
Following axotomy, the contact between motoneurons and muscle fibers is disrupted, triggering a retrograde reaction at the neuron cell body within the spinal cord. Together with chromatolysis, a hallmark of such response to injury is the elimination of presynaptic terminals apposing to the soma and proximal dendrites of the injured neuron. Excitatory inputs are preferentially eliminated, leaving the cells under an inhibitory influence during the repair process. This is particularly important to avoid glutamate excitotoxicity...
September 2015: Neuropharmacology
https://www.readbyqxmd.com/read/25338642/functional-regeneration-of-supraspinal-connections-in-a-patient-with-transected-spinal-cord-following-transplantation-of-bulbar-olfactory-ensheathing-cells-with-peripheral-nerve-bridging
#19
Pawel Tabakow, Geoffrey Raisman, Wojciech Fortuna, Marcin Czyz, Juliusz Huber, Daqing Li, Pawel Szewczyk, Stefan Okurowski, Ryszard Miedzybrodzki, Bogdan Czapiga, Beata Salomon, Agnieszka Halon, Ying Li, Joanna Lipiec, Aleksandra Kulczyk, Wlodzimierz Jarmundowicz
Treatment of patients sustaining a complete spinal cord injury remains an unsolved clinical problem because of the lack of spontaneous regeneration of injured central axons. A 38-year-old man sustained traumatic transection of the thoracic spinal cord at upper vertebral level Th9. At 21 months after injury, the patient presented symptoms of a clinically complete spinal cord injury (American Spinal Injury Association class A-ASIA A). One of the patient's olfactory bulbs was removed and used to derive a culture containing olfactory ensheathing cells and olfactory nerve fibroblasts...
2014: Cell Transplantation
https://www.readbyqxmd.com/read/25322603/-efficacy-of-co2-laser-in-the-treatment-of-precancerous-laryngeal-lesions-under-phonomicrosurgery-and-its-relative-factors
#20
Qingjia Gu, Yong Feng, Xiaoxu Yu, Jian'gang Fan, Debing Li, Gang He
OBJECTIVE: To investigate the efficacy of CO2 laser treatment for patients with precancerous laryngeal lesions under phonomicrosurgery and to explore the points for attention in operation. METHOD: They were all treated with phonomicrosurgery techniques as mucosal epitheliumablation or mucosal stripping by using CO2 laser. Eight patients with laryngeal papilloma were excised by CO2 laser. RESULT: All patients were treated with CO2 laser surgery successfully...
August 2014: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
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