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Sabrina Klein, Dennis Nurjadi, Susanne Horner, Klaus Heeg, Stefan Zimmermann, Irene Burckhardt
While total laboratory automation (TLA) is well established in laboratory medicine, only a few microbiological laboratories are using TLA systems. Especially in terms of speed and accuracy, working with TLA is expected to be superior to conventional microbiology. We compared in total 35,564 microbiological urine cultures with and without incubation and processing with BD Kiestra TLA for a 6-month period each retrospectively. Sixteen thousand three hundred thirty-eight urine samples were analyzed in the pre-TLA period and 19,226 with TLA...
April 13, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Antony Croxatto, Raphaël Marcelpoil, Cédrick Orny, Didier Morel, Guy Prod'hom, Gilbert Greub
BACKGROUND: Automation in microbiology laboratories impacts management, workflow, productivity and quality. Further improvements will be driven by the development of intelligent image analysis allowing automated detection of microbial growth, release of sterile samples, identification and quantification of bacterial colonies and reading of AST disk diffusion assays. We investigated the potential benefit of intelligent imaging analysis by developing algorithms allowing automated detection, semi-quantification and identification of bacterial colonies...
December 2017: Biomedical Journal
Talent Theparee, Sanchita Das, Richard B Thomson
Technological advances have changed the practice of clinical microbiology. We implemented Bruker matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) and BD Kiestra total laboratory automation (TLA) 4 and 3 years ago, respectively. To assess the impact of these new technologies, we compared turnaround times (TATs) for positive and negative urine cultures before and after implementation. In comparison I, TATs for 61,157 urine cultures were extracted for two periods corresponding to pre-TLA and post-TLA, both using MALDI-TOF MS for organism identification...
January 2018: Journal of Clinical Microbiology
C S Heather, M Maley
Early detection of resistance in sepsis due to Gram-negative organisms may lead to improved outcomes by reducing the time to effective antibiotic therapy. Traditional methods of resistance detection require incubation times of 18 to 48 h to detect resistance. We have utilised automated specimen processing, digital imaging and zone size measurements in conjunction with direct disc susceptibility testing to develop a method for the rapid screening of Gram-negative blood culture isolates for resistance. Positive clinical blood cultures with Gram-negative organisms were prospectively identified and additional resistant mock specimens were prepared...
January 2018: European Journal of Clinical Microbiology & Infectious Diseases
Inga Fröding, Martin Vondracek, Christian G Giske
Objectives: The need for rapid antibiotic susceptibility testing increases with escalating levels of antimicrobial resistance in Enterobacteriaceae. Our objective was to evaluate the accuracy of reading EUCAST disc diffusion, ROSCO ESBL and carbapenemase detection kits and the Mast Carbapenemase Activity Test (CAT-ID) disc, after 6 h of incubation. Methods: We used a collection of 128 isolates of Escherichia coli and Klebsiella pneumoniae with a wide variety of resistance mechanisms...
April 1, 2017: Journal of Antimicrobial Chemotherapy
Maryza Graham, Leanne Tilson, Richard Streitberg, John Hamblin, Tony M Korman
We compared the results of 505 urine specimens prospectively processed by both conventional manual processing (MP) with 16-24h incubation to BD Kiestra™ Total Laboratory Automation (TLA) system with a shortened incubation of 14h: 97% of culture results were clinically concordant. TLA processing was associated with improved standardization of time of first culture reading and total incubation time.
September 2016: Diagnostic Microbiology and Infectious Disease
A Croxatto, G Prod'hom, F Faverjon, Y Rochais, G Greub
Automation was introduced many years ago in several diagnostic disciplines such as chemistry, haematology and molecular biology. The first laboratory automation system for clinical bacteriology was released in 2006, and it rapidly proved its value by increasing productivity, allowing a continuous increase in sample volumes despite limited budgets and personnel shortages. Today, two major manufacturers, BD Kiestra and Copan, are commercializing partial or complete laboratory automation systems for bacteriology...
March 2016: Clinical Microbiology and Infection
Jesper Iversen, Gitta Stendal, Cecilie M Gerdes, Christian H Meyer, Christian Østergaard Andersen, Niels Frimodt-Møller
This study evaluated the quantitative results from and quality of the inoculation patterns of urine specimens produced by two automated instruments, the Copan WASP and the BD InoqulA. Five hundred twenty-six urine samples submitted in 10-ml canisters containing boric acid were processed within 30 min on an InoqulA instrument plating 10 μl of specimen, and on two WASP instruments, one plating 1 μl of specimen (WASP-1), and the second plating 10 μl of WASP (WASP-10). All samples were incubated, analyzed, and digitally imaged using the BD Kiestra total lab automation system...
February 2016: Journal of Clinical Microbiology
Michael Hombach, Florian P Maurer, Tamara Pfiffner, Erik C Böttger, Reinhard Furrer
Parameters like zone reading, inoculum density, and plate streaking influence the precision and accuracy of disk diffusion antibiotic susceptibility testing (AST). While improved reading precision has been demonstrated using automated imaging systems, standardization of the inoculum and of plate streaking have not been systematically investigated yet. This study analyzed whether photometrically controlled inoculum preparation and/or automated inoculation could further improve the standardization of disk diffusion...
December 2015: Journal of Clinical Microbiology
Antony Croxatto, Klaas Dijkstra, Guy Prod'hom, Gilbert Greub
The quality of sample inoculation is critical for achieving an optimal yield of discrete colonies in both monomicrobial and polymicrobial samples to perform identification and antibiotic susceptibility testing. Consequently, we compared the performance between the InoqulA (BD Kiestra), the WASP (Copan), and manual inoculation methods. Defined mono- and polymicrobial samples of 4 bacterial species and cloudy urine specimens were inoculated on chromogenic agar by the InoqulA, the WASP, and manual methods. Images taken with ImagA (BD Kiestra) were analyzed with the VisionLab version 3...
July 2015: Journal of Clinical Microbiology
Sharon Strauss, Paul P Bourbeau
This study compared results from plating urine specimens with the BD InoqulA instrument using a 10-μl inoculum with results from cultures plated manually with a 1-μl loop for comparable 2-month periods. The positivity rates, turnaround times for positive cultures, and BD Phoenix identification and antimicrobial susceptibility test results were comparable for both time periods. We experienced no problems with culture interpretation as the result of moving to the 10-μl inoculum.
May 2015: Journal of Clinical Microbiology
Nico T Mutters, Caspar J Hodiamont, Menno D de Jong, Hendri P J Overmeijer, Mandy van den Boogaard, Caroline E Visser
BACKGROUND: Microbiological laboratories seek technologically innovative solutions to cope with large numbers of samples and limited personnel and financial resources. One platform that has recently become available is the Kiestra Total Laboratory Automation (TLA) system (BD Kiestra B.V., the Netherlands). This fully automated sample processing system, equipped with digital imaging technology, allows superior detection of microbial growth. Combining this approach with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MS) (Bruker Daltonik, Germany) is expected to enable more rapid identification of pathogens...
March 2014: Annals of Laboratory Medicine
P Froment, H Marchandin, P Vande Perre, B Lamy
The process of plate streaking has been automated to improve the culture readings, isolation quality, and workflow of microbiology laboratories. However, instruments have not been well evaluated under routine conditions. We aimed to evaluate the performance of the fully automated InoqulA instrument (BD Kiestra B.V., The Netherlands) in the automated seeding of liquid specimens and samples collected using swabs with transport medium. We compared manual and automated methods according to the (i) within-run reproducibility using Escherichia coli-calibrated suspensions, (ii) intersample contamination using a series of alternating sterile broths and broths with >10(5) CFU/ml of either E...
March 2014: Journal of Clinical Microbiology
G Greub, G Prod'hom
With increased activity and reduced financial and human resources, there is a need for automation in clinical bacteriology. Initial processing of clinical samples includes repetitive and fastidious steps. These tasks are suitable for automation, and several instruments are now available on the market, including the WASP (Copan), Previ-Isola (BioMerieux), Innova (Becton-Dickinson) and Inoqula (KIESTRA) systems. These new instruments allow efficient and accurate inoculation of samples, including four main steps: (i) selecting the appropriate Petri dish; (ii) inoculating the sample; (iii) spreading the inoculum on agar plates to obtain, upon incubation, well-separated bacterial colonies; and (iv) accurate labelling and sorting of each inoculated media...
May 2011: Clinical Microbiology and Infection
S Matthews, J Deutekom
Diagnostic bacteriology has changed little over the past 50 years. Those changes that have occurred have been gradual but not fundamental. KIESTRA Lab Automation is a company specializing in total laboratory automation (TLA). Their methodology incorporates plate labelling and delivery, inoculation and spreading, incubation and digital image analysis. This has led to a paradigm shift in processing since decisions are made from images on a computer screen rather than examination of the actual plates. The result is the development of a skill mix based on the requirements of the service...
May 2011: Clinical Microbiology and Infection
Femke Kiestra, Trudi Miltenburg, Peter van Beukelen
No abstract text is available yet for this article.
November 15, 2010: Tijdschrift Voor Diergeneeskunde
Femke Kiestra
No abstract text is available yet for this article.
January 1, 2010: Tijdschrift Voor Diergeneeskunde
I M J Mathijssen, H Strijdhorst, S K Kiestra, J C J Wereldsma
BACKGROUND: For staging purposes in breast cancer it is current practice to perform a sentinel node biopsy in a clinically negative axilla, followed by an axillary lymph node dissection if metastases are found in the sentinel node. To limit the number of surgical procedures it is therefore of importance to try and identify as much patients as possible who have axillary metastases. Clinical staging of the axillary nodes in breast cancer is mainly based on palpation, but ultrasound has been shown to be of additional value in detecting pathological nodes...
October 1, 2006: Journal of Surgical Oncology
No abstract text is available yet for this article.
November 18, 1961: Nederlands Tijdschrift Voor Geneeskunde
No abstract text is available yet for this article.
September 30, 1961: Nederlands Tijdschrift Voor Geneeskunde
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