Michael D Roberts, Bradley A Ruple, Joshua S Godwin, Mason C McIntosh, Shao-Yung Chen, Nicholas J Kontos, Anthony Agyin-Birikorang, Max Michel, Daniel L Plotkin, Madison L Mattingly, Brooks Mobley, Tim N Ziegenfuss, Andrew D Fruge, Andreas N Kavazis
The skeletal muscle proteome alterations to aging and resistance training have been reported in prior studies. However, conventional proteomics in skeletal muscle typically yields wide protein abundance ranges that mask the detection of lowly expressed proteins. Thus, we adopted a novel deep proteomics approach whereby myofibril (MyoF) and non-MyoF fractions were separately subjected to protein corona nanoparticle complex formation prior to digestion and Liquid Chromatography Mass Spectrometry (LC-MS). Specifically, we investigated MyoF and non-MyoF proteomic profiles of the vastus lateralis muscle of younger (Y, 22±2 years old; n=5) and middle-aged participants (MA, 56±8 years old; n=6)...
April 19, 2024: Aging