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https://www.readbyqxmd.com/read/27911386/a-protocol-for-safe-lithiation-reactions-using-organolithium-reagents
#1
Michael R Gau, Michael J Zdilla
Organolithium reagents are powerful tools in the synthetic chemist's toolbox. However, the extreme pyrophoric nature of the most reactive reagents warrants proper technique, thorough training, and proper personal protective equipment. To aid in the training of researchers using organolithium reagents, a thorough, step-by-step protocol for the safe and effective use of tert-butyllithium on an inert gas line or within a glovebox is described. As a model reaction, preparation of lithium tert-butyl amide by the reaction of tert-butyl amine with one equivalent of tert-butyl lithium is presented...
November 12, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27911038/quantitating-the-cell-turning-images-into-numbers-with-imagej
#2
REVIEW
Ellen T Arena, Curtis T Rueden, Mark C Hiner, Shulei Wang, Ming Yuan, Kevin W Eliceiri
Modern biological research particularly in the fields of developmental and cell biology has been transformed by the rapid evolution of the light microscope. The light microscope, long a mainstay of the experimental biologist, is now used for a wide array of biological experimental scenarios and sample types. Much of the great developments in advanced biological imaging have been driven by the digital imaging revolution with powerful processors and algorithms. In particular, this combination of advanced imaging and computational analysis has resulted in the drive of the modern biologist to not only visually inspect dynamic phenomena, but to quantify the involved processes...
December 2, 2016: Wiley Interdisciplinary Reviews. Developmental Biology
https://www.readbyqxmd.com/read/27910600/development-of-a-neural-network-technique-for-kstar-thomson-scattering-diagnostics
#3
Seung Hun Lee, J H Lee, I Yamada, Jae Sun Park
Neural networks provide powerful approaches of dealing with nonlinear data and have been successfully applied to fusion plasma diagnostics and control systems. Controlling tokamak plasmas in real time is essential to measure the plasma parameters in situ. However, the χ(2) method traditionally used in Thomson scattering diagnostics hampers real-time measurement due to the complexity of the calculations involved. In this study, we applied a neural network approach to Thomson scattering diagnostics in order to calculate the electron temperature, comparing the results to those obtained with the χ(2) method...
November 2016: Review of Scientific Instruments
https://www.readbyqxmd.com/read/27909886/automatic-snoring-sounds-detection-from-sleep-sounds-via-multi-features-analysis
#4
Can Wang, Jianxin Peng, Lijuan Song, Xiaowen Zhang
Obstructive sleep apnea hypopnea syndrome (OSAHS) is a serious respiratory disorder. Snoring is the most intuitively characteristic symptom of OSAHS. Recently, many studies have attempted to develop snore analysis technology for diagnosing OSAHS. The preliminary and essential step in such diagnosis is to automatically segment snoring sounds from original sleep sounds. This study presents an automatic snoring detection algorithm that detects potential snoring episodes using an adaptive effective-value threshold method, linear and nonlinear feature extraction using maximum power ratio, sum of positive/negative amplitudes, 500 Hz power ratio, spectral entropy (SE) and sample entropy (SampEn), and automatic snore/nonsnore classification using a support vector machine...
December 1, 2016: Australasian Physical & Engineering Sciences in Medicine
https://www.readbyqxmd.com/read/27904845/cardio-thoracic-surgical-experience-in-gabon
#5
REVIEW
François Ondo N'Dong, Sylvestre Mbamendame, Silvère Ngakani Offobo, Mory Mamadi Kaba, Jean Bernard Mbourou, Charles Diané
Our experience in cardio-thoracic surgery focuses on thoracic activity. The minimum fare for traumatisms, infectious pathology and tumoral pathology requires, for its improvement, the acquisition of a technical platform and of an adequate medical infrastructure, with a rational organisation of the care sequence. Vascular surgery calls for the training of qualified human resources, and the great demand in heart surgery calls upon the public powers for the construction of infrastructures, and the formation of necessary superstructures...
October 2016: Cardiovascular Diagnosis and Therapy
https://www.readbyqxmd.com/read/27904613/the-facilitators-and-impediment-factors-of-midwifery-student-s-empowerment-in-pregnancy-and-delivery-care-a-qualitative-study
#6
Mojgan Janighorban, Nikoo Yamani, Hojatollah Yousefi
BACKGROUND: The organizational environment and its existing context may deeply affect on empowerment of individuals. In educational institutions as well as other organizations, students are going to be powerful when opportunities for growth and achievement of power are provided for them in learning and educational environments. This study has been carried out to explain the facilitators and impediment factors of midwifery student's empowerment in pregnancy and delivery care. MATERIALS AND METHODS: The current qualitative study has been conducted with participation of 15 midwifery senior students, 10 midwifery academic teachers, and 2 employed midwives in educational hospitals...
2016: Journal of Research in Medical Sciences: the Official Journal of Isfahan University of Medical Sciences
https://www.readbyqxmd.com/read/27899799/population-health-based-approaches-to-utilizing-digital-technology-a-strategy-for-equity
#7
Garth N Graham, MaryLynn Ostrowski, Alyse B Sabina
Health care disparities and high chronic disease rates burden many communities and disproportionally impact racial/ethnic populations in the United States. These disparities vary geographically, increase health care expenses, and result in shortened lifespans. Digital technologies may be one tool for addressing health disparities and improving population health by increasing individuals' access to health information-especially as most low-income U.S. residents gain access to smartphones. The Aetna Foundation partners with organizations to use digital technologies, including mobile applications, data collection, and related platforms, for learning and sharing...
November 2016: Journal of Public Health Policy
https://www.readbyqxmd.com/read/27899588/a-high-throughput-approach-to-profile-rna-structure
#8
Riccardo Delli Ponti, Stefanie Marti, Alexandros Armaos, Gian Gaetano Tartaglia
Here we introduce the Computational Recognition of Secondary Structure (CROSS) method to calculate the structural profile of an RNA sequence (single- or double-stranded state) at single-nucleotide resolution and without sequence length restrictions. We trained CROSS using data from high-throughput experiments such as Selective 2'-Hydroxyl Acylation analyzed by Primer Extension (SHAPE; Mouse and HIV transcriptomes) and Parallel Analysis of RNA Structure (PARS; Human and Yeast transcriptomes) as well as high-quality NMR/X-ray structures (PDB database)...
November 28, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27898810/genomic-selection-for-processing-and-end-use-quality-traits-in-the-cimmyt-spring-bread-wheat-breeding-program
#9
Sarah D Battenfield, Carlos Guzmán, R Chris Gaynor, Ravi P Singh, Roberto J Peña, Susanne Dreisigacker, Allan K Fritz, Jesse A Poland
Wheat ( L.) cultivars must possess suitable end-use quality for release and consumer acceptability. However, breeding for quality traits is often considered a secondary target relative to yield largely because of amount of seed needed and expense. Without testing and selection, many undesirable materials are advanced, expending additional resources. Here, we develop and validate whole-genome prediction models for end-use quality phenotypes in the CIMMYT bread wheat breeding program. Model accuracy was tested using forward prediction on breeding lines ( = 5520) tested in unbalanced yield trials from 2009 to 2015 at Ciudad Obregon, Sonora, Mexico...
July 2016: Plant Genome
https://www.readbyqxmd.com/read/27898641/oral-contraceptive-use-dampens-physiological-adaptations-to-sprint-interval-training
#10
Mia A Schaumberg, David G Jenkins, Xanne A K Janse de Jonge, Lynne M Emmerton, Tina L Skinner
PURPOSE: Oral contraceptive (OC) use reduces peak aerobic capacity (V˙O2peak), however, whether it also influences adaptations to training has yet to be determined. This study aimed to examine the influence of OC use on peak performance [peak power output (PPO)] and physiological adaptations [V˙O2peak and peak cardiac output (Q˙peak)] following sprint interval training (SIT) in recreationally-active women. METHODS: Women taking an OC (n=25) or experiencing natural regular menstrual cycles (MC; n=16) completed an incremental exercise test to assess V˙O2peak, PPO, and Q˙peak before, immediately after, and four weeks following 12 sessions of SIT...
November 28, 2016: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/27897989/periodization-of-carbohydrate-intake-short-term-effect-on-performance
#11
Laurie-Anne Marquet, Christophe Hausswirth, Odeline Molle, John A Hawley, Louise M Burke, Eve Tiollier, Jeanick Brisswalter
BACKGROUND: "Sleep-low" consists of a sequential periodization of carbohydrate (CHO) availability-low glycogen recovery after "train high" glycogen-depleting interval training, followed by an overnight-fast and light intensity training ("train low") the following day. This strategy leads to an upregulation of several exercise-responsive signaling proteins, but the chronic effect on performance has received less attention. We investigated the effects of short-term exposure to this strategy on endurance performance...
November 25, 2016: Nutrients
https://www.readbyqxmd.com/read/27897397/development-and-validation-of-a-septoplasty-training-model-using-3-dimensional-printing-technology
#12
Mahmoud A AlReefi, Lily H P Nguyen, Luc G Mongeau, Bassam Ul Haq, Siddharth Boyanapalli, Nauman Hafeez, Francois Cegarra-Escolano, Marc A Tewfik
BACKGROUND: Providing alternative training modalities may improve trainees' ability to perform septoplasty. Three-dimensional printing has been shown to be a powerful tool in surgical training. The objectives of this study were to explain the development of our 3-dimensional (3D) printed septoplasty training model, to assess its face and content validity, and to present evidence supporting its ability to distinguish between levels of surgical proficiency. METHODS: Imaging data of a patient with a nasal septal deviation was selected for printing...
November 29, 2016: International Forum of Allergy & Rhinology
https://www.readbyqxmd.com/read/27896960/on-the-power-and-limits-of-sequence-similarity-based-clustering-of-proteins-into-families
#13
Christian Wiwie, Richard Röttger
Over the last decades, we have observed an ongoing tremendous growth of available sequencing data fueled by the advancements in wet-lab technology. The sequencing information is only the beginning of the actual understanding of how organisms survive and prosper. It is, for instance, equally important to also unravel the proteomic repertoire of an organism. A classical computational approach for detecting protein families is a sequence-based similarity calculation coupled with a subsequent cluster analysis. In this work we have intensively analyzed various clustering tools on a large scale...
2016: Pacific Symposium on Biocomputing
https://www.readbyqxmd.com/read/27896682/methods-of-power-force-velocity-profiling-during-sprint-running-a-narrative-review
#14
REVIEW
Matt R Cross, Matt Brughelli, Pierre Samozino, Jean-Benoit Morin
The ability of the human body to generate maximal power is linked to a host of performance outcomes and sporting success. Power-force-velocity relationships characterize limits of the neuromuscular system to produce power, and their measurement has been a common topic in research for the past century. Unfortunately, the narrative of the available literature is complex, with development occurring across a variety of methods and technology. This review focuses on the different equipment and methods used to determine mechanical characteristics of maximal exertion human sprinting...
November 28, 2016: Sports Medicine
https://www.readbyqxmd.com/read/27896514/tumor-infiltrating-mast-cells-tims-confers-a-marked-survival-advantage-in-nonmetastatic-clear-cell-renal-cell-carcinoma
#15
Hangcheng Fu, Yu Zhu, Yiwei Wang, Zheng Liu, Junyu Zhang, Zewei Wang, Huyang Xie, Bo Dai, Jiejie Xu, Dingwei Ye
PURPOSE: The role played by the innate immune system in determining the clinical outcome of clear-cell renal cell carcinoma (ccRCC) was still blurred. This study was designed to investigate the prognostic significance of tumor infiltrating mast cells (TIMs) in ccRCC. METHODS: The study retrospectively enrolled a training set (474 patients) and a validation set (188 patients) with nonmetastasis (pT1-4N0M0) ccRCC from two institutional medical centers of China. TIMs was evaluated by immunohistochemical staining of tryptase and its association with clinicopathologic features and prognosis were evaluated...
November 28, 2016: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/27895529/assessing-nursing-staff-s-competences-in-mobility-support-in-nursing-home-care-development-and-psychometric-testing-of-the-kinaesthetics-competence-kc-observation-instrument
#16
Heidrun Gattinger, Helena Leino-Kilpi, Virpi Hantikainen, Sascha Köpke, Stefan Ott, Beate Senn
BACKGROUND: Between 75 and 89% of residents living in long-term care facilities have limited mobility. Nurses as well as other licensed and unlicensed personnel directly involved in resident care are in a key position to promote and maintain the mobility of care-dependent persons. This requires a certain level of competence. Kinaesthetics is a training concept used to increase nursing staff's interaction and movement support skills for assisting care-dependent persons in their daily activities...
2016: BMC Nursing
https://www.readbyqxmd.com/read/27893480/high-speed-resistance-training-modifies-load-velocity-and-load-power-relationships-in-parkinson-s-disease
#17
Meng Ni, Joseph F Signorile
Muscle power is a major neuromuscular factor affecting motor function and independence in patients with Parkinson's disease; and it is commonly targeted using high-speed exercise. This study examined the changes in velocities (Vpp) and percent loads (%1RMpp) at peak power, as well as load-velocity (L-V) and load-power (L-P) relationships, resulting from resistance training due to exercise choice and loading in older Parkinson's patients. Fourteen older adults with mild to moderate Parkinson's disease participated in a 12-week randomized controlled power training trial...
November 16, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27893479/complex-training-the-effect-of-exercise-selection-and-training-status-on-post-activation-potentiation-in-rugby-league-players
#18
David J Scott, Massimiliano Ditroilo, Phil Marshall
This study compared the post-activation potentiation (PAP) response of the hex bar deadlift (HBD) and back squat (BS) exercises. The PAP response between different levels of athletes was also compared. Ten professional and ten amateur rugby league players performed two experimental sessions. Participants performed a countermovement jump (CMJ) before and 2, 4, 6, 8, 10, 12, 14 and 16 minutes after a conditioning activity (CA) that contained 1 set of 3 repetitions at 93% 1RM of either HBD or BS. A force platform determined peak power output (PPO), force at PPO, velocity at PPO and jump height of each CMJ...
November 16, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27893478/loads-and-movement-speeds-dictate-differences-in-power-output-during-circuit-training
#19
Kirk B Roberson, Sean Chowdhari, Morgan White, Joseph F Signorile
Power training has become a common exercise intervention for improving muscle strength, power, and physical function, while reducing injury risk. Few studies, however, have evaluated acute load changes on power output during traditional resistance-training protocols. Therefore, the aim of this study was to quantify the effects of different loading patterns on power output during a single session of circuit resistance training (CRT). Nine males (age=19.4±0.9 y) and eleven females (age=20.6±1.6 y) completed three CRT protocols during separate testing sessions using seven pneumatic exercises...
November 16, 2016: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/27892471/combining-multiple-hypothesis-testing-with-machine-learning-increases-the-statistical-power-of-genome-wide-association-studies
#20
Bettina Mieth, Marius Kloft, Juan Antonio Rodríguez, Sören Sonnenburg, Robin Vobruba, Carlos Morcillo-Suárez, Xavier Farré, Urko M Marigorta, Ernst Fehr, Thorsten Dickhaus, Gilles Blanchard, Daniel Schunk, Arcadi Navarro, Klaus-Robert Müller
The standard approach to the analysis of genome-wide association studies (GWAS) is based on testing each position in the genome individually for statistical significance of its association with the phenotype under investigation. To improve the analysis of GWAS, we propose a combination of machine learning and statistical testing that takes correlation structures within the set of SNPs under investigation in a mathematically well-controlled manner into account. The novel two-step algorithm, COMBI, first trains a support vector machine to determine a subset of candidate SNPs and then performs hypothesis tests for these SNPs together with an adequate threshold correction...
November 28, 2016: Scientific Reports
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