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https://www.readbyqxmd.com/read/29334660/global-proteomic-analysis-of-water-buffalo-bubalus-bubalis-saliva-at-different-stages-of-estrous-cycle-using-high-throughput-mass-spectrometry
#1
N G Shashikumar, R K Baithalu, S Bathla, S A Ali, P Rawat, A Kumaresan, S Kumar, B R Maharana, G Singh, D S Puneeth Kumar, S K Singh, S S Lathwal, L Jaiswal, T K Mohanty, A K Mohanty
Accurate and efficient detection of estrus is one of the major constraints for exploitation of the production potential of buffalo owing to its poor manifestation of estrus signs, seasonal differences in expression and higher incidences of silent estrus (29%). The current study focused on identification of estrus specific candidate proteins in saliva of buffaloes. Estrus was detected based on behavioral signs in response to the teaser and changes in reproductive organs and confirmed by per-rectal examination, trans-rectal USG of reproductive organs, cervico-vaginal mucus characteristics and blood serum progesterone estimation...
January 9, 2018: Theriogenology
https://www.readbyqxmd.com/read/29330998/histomorphological-effects-of-hunger-stress-on-ovaries
#2
Maria Yousaf, Hina Siddiqi, Nazish Waheed
BACKGROUND: Incidence of stress is on the rise in our daily life involving various neurobiological, endocrinological and behavioral changes. Hunger stress has a potent influence on mental, physical, and reproductive health by affecting the hypothalamic-pituitary gonadal axis. METHODS: It was a laboratory based randomized control trial. Adult female mice (BALB-c strain) weighing 25-27 grams on first day of estrous cycle were taken in two groups (ten each). Group A was kept in normal environment of animal house for one month...
October 2017: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/29324978/effect-of-fish-meal-supplementation-on-luteal-sensitivity-to-intrauterine-infusions-of-prostaglandin-f2alpha-in-the-bovine
#3
Michele R Plewes, Jessica C Cedillo, Patrick D Burns, Peter E Graham, Jason E Bruemmer, Terry E Engle
Progesterone is a steroid hormone secreted from the corpus luteum (CL), which is responsible for establishment and maintenance of pregnancy. Early embryonic mortality often occurs due to inadequate regulation of uterine prostaglandin (PG) F2alpha secretion, leading to decrease in progesterone and loss of pregnancy. The objective of the current study was to determine the effects of fish meal supplementation on luteal sensitivity to intrauterine infusions of PGF2alpha. Non-lactating beef cows received corn gluten meal or fish meal supplementation for 60-days...
January 9, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29318744/immunoisolating-poly-ethylene-glycol-based-capsules-support-ovarian-tissue-survival-to-restore-endocrine-function
#4
James R Day, Anu David, Alexa L Cichon, Tanay Kulkarni, Marilia Cascalho, Ariella Shikanov
A common irreversible adverse effect of life-saving anticancer treatments is loss of gonadal endocrine function and fertility, calling for a need to focus on post-treatment quality of life. Here, we investigated the use of poly(ethylene glycol)-vinyl sulfone based capsules to support syngeneic donor ovarian tissue for restoration of endocrine function in mice. We designed a dual immunoisolating capsule (PEG-Dual) by tuning the physical properties of the PEG hydrogels and combining proteolytically degradable (PEG-PD) and non-degradable (PEG-NPD) layers to meet the numerous requirements for encapsulation and immunoisolation of ovarian tissue, such as nutrient diffusion and tissue expansion...
January 10, 2018: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/29311525/evaluating-the-electrical-impedance-and-mucus-related-gene-expression-of-uterine-endometrial-tissues-in-mares
#5
Kohta Kikuchi, Keisuke Kozai, Takuo Hojo, Miki Sakatani, Kiyoshi Okuda, Hanako Bai, Manabu Kawahara, Masashi Takahashi
We investigated the electrical impedance of the reproductive tracts (vagina and uterine endometrial tissues) and the expression of mucus-related genes to identify the stage of the estrous cycle in mares. We first examined vaginal impedance in native Hokkaido mares during their estrous cycle and found no significant differences. However, impedance levels tended to decrease towards ovulation. Furthermore, we investigated the estrous cycle by measuring the electrical impedance of the uterine endometrial tissues obtained from carcasses of mares...
January 7, 2018: Journal of Reproduction and Development
https://www.readbyqxmd.com/read/29305268/long-term-estradiol-17%C3%AE-exposure-decreases-the-cholinergic-innervation-pattern-of-the-pig-ovary
#6
B Jana, K A Meller, M Czajkowska, J Całka
Elevated levels of endogenous estrogens in the course of pathological states of ovaries, as well as xenoestrogens, may lead to hyperestrogenism. It has previously been demonstrated that long-term estradiol-17β (E2) administration in adult gilts affected the population of sympathetic intraovarian nerve fibers. The aim of this study has been to determine the effect of long-term E2 exposure on the cholinergic innervation pattern of porcine ovaries. Intraovarian distribution and the density of nerve fibers immunoreactive (IR) to vesicular acetylocholine transporter (VAChT) and/or neuronal isoform of nitric oxide synthase (nNOS), vasoactive intestinal polypeptide (VIP), somatostatin (SOM) were determined...
January 2, 2018: Annals of Anatomy, Anatomischer Anzeiger: Official Organ of the Anatomische Gesellschaft
https://www.readbyqxmd.com/read/29297778/chronic-cholestasis-is-associated-with-hypogonadism-and-premature-ovarian-failure-in-adult-rats-cholestasis-causes-ovarian-hypogonadism
#7
Yomna I Mahmoud
Hypogonadism is a well-known complication in males with chronic liver diseases. However, the consequences of chronic hepatopathies on female reproductive capacities have received relatively little attention. The present study evaluates the effect of chronic obstructive jaundice on the ovary of adult cycling rats. Estrous cyclicity was monitored to check the functional status of the hypothalamic-pituitary-ovarian axis. Ovarian changes were assessed using histomorphometric, immunohistochemical, and ultrastructural techniques...
January 2018: Ultrastructural Pathology
https://www.readbyqxmd.com/read/29287623/olfactory-stimulation-or-inhibition-of-sexual-behavior-of-stallions-in-non-breeding-season
#8
Daniel Guillaume, Chantal Moussu, Frédéric de Geoffroy, Didier Chesneau, Matthieu Keller
Stallions show decreased sexual responses and activities during short days in winter. To evaluate the importance of sexual olfactory communication in horses, we tested whether sexual responses could be stimulated through various sexual olfactory stimulations in winter. To this end, we presented stallions with various olfactory stimulations (urine from mares at different stages of the reproductive cycle, urine from stallions or geldings, or chemically defined synthetic odorant) during the non-breeding season and measured their behavioral responses through (1) a test of olfactory investigation (olfactory investigation and flehmen behavior) and (2) a test of sexual activity in the context of semen collection for artificial insemination...
December 26, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29284211/ovary-and-vaginal-epithelium-dynamic-during-the-estrous-cycle-on-dasyprocta-prymnolopha-wagler-1831-by-ultrasound-and-cytological-examination
#9
Artur N Carreiro, João A R Alves Diniz, Joyce G de Souza, Débora V Fernandes de Araújo, Rômulo F Francelino Dias, Liliane M Dos Santos Azerêdo, Ediane F Rocha, Ana Yasha F de La Salles, Carlos E Peña Alfaro, Maria A Martins de Carvalho, Maria J Illera, Danilo J Ayres de Menezes
The objective of the present study was to follow and characterize the morphological alterations in ovaries of agouti (Dasyprocta prymnolopha), reared in captivity, by abdominal ultrasonography. All the animals were submitted daily to vaginal cytology to identify the current cycle phases. For each phase, ultrasound examinations were carried out to identify and describe the morphology of both the ovaries regarding each phase of the estrus cycle, establishing topographic parameters in an ultrasound window to locate them...
December 28, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/29278679/cell-specific-expression-and-signal-transduction-of-c-c-motif-chemokine-ligand-2-and-atypical-chemokine-receptors-in-the-porcine-endometrium-during-early-pregnancy
#10
Whasun Lim, Hyocheol Bae, Fuller W Bazer, Gwonhwa Song
Chemokines and atypical chemokine receptors (ACKRs; also known as chemokine decoy receptors) play an important role in reproductive immunology by recruiting leukocytes during early pregnancy. The aim of this study was to determine the expression of C-C motif chemokine ligand 2 (CCL2) and ACKRs in the endometrium during estrous cycle and early pregnancy, and to investigate the functional effects of CCL2 on porcine uterine luminal epithelial (pLE) cells. Our results indicated that CCL2, ACKR1, ACKR3, and ACKR4 were strongly detected in the glandular and luminal epithelium of the endometrium during early pregnancy compared to that in non-pregnant pigs...
December 23, 2017: Developmental and Comparative Immunology
https://www.readbyqxmd.com/read/29274741/a-novel-therapeutic-approach-for-treatment-of-catamenial-epilepsy
#11
Suchitra Joshi, Huayu Sun, Karthik Rajasekaran, John Williamson, Edward Perez-Reyes, Jaideep Kapur
Many women with epilepsy experience perimenstrual seizure exacerbation, referred to as catamenial epilepsy. There is no effective treatment for this condition, proposed to result from withdrawal of neurosteroid-mediated effects of progesterone. A double-blind, multicenter, phase III, clinical trial of catamenial epilepsy has failed to find a beneficial effect of progesterone. The neurosteroid-mediated effects of progesterone have been extensively studied in relation to catamenial epilepsy; however, the effects mediated by progesterone receptor activation have been overlooked...
December 21, 2017: Neurobiology of Disease
https://www.readbyqxmd.com/read/29273681/sorcin-is-involved-during-embryo-implantation-via-activating-vegf-pi3k-akt-pathway-in-mice
#12
Kanchan Gupta, Vijay Kumar Sirohi, Suparna Kumari, Vinay Shukla, Murli Manohar, Pooja Popli, Anila Dwivedi
Earlier studies have demonstrated the cyclic variation and also the altered expression of sorcin in endometrium during early- to mid-secretory phase transition in women with unexplained infertility. The current study was undertaken to establish the functional role of sorcin in endometrial receptivity in mice. Results indicated that sorcin was highly expressed during the window of implantation in mice and functional blockage of sorcin caused significant reduction in number of implanted blastocyst. The receptivity markers (i...
December 22, 2017: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29273454/effects-of-non-contingent-cocaine-on-3-alpha-androstanediol-ii-disruption-of-lordosis-of-proestrous-rats
#13
Amy S Kohtz, Alicia A Walf, Cheryl A Frye
Drug use influences sexual behavior, performance, and can be associated with increased sexual risk-taking. Our prior results using an animal model indicate that progestogens contribute to hormonally-mediated changes in sexual behavior of female rodents during acute cocaine exposure. Androgens, such as testosterone, and its metabolite 3ɑ-androstanediol (3α-diol), and estradiol, are known to influence male sexual behavior, but can also alter the expression of sexual behavior of female rodents. As such, we investigated the influence of endogenous androgen and estradiol fluctuations on cocaine-mediated changes in motor behavior and sexual receptivity of rats during diestrous or proestrous phases of the estrous cycle...
December 19, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/29273437/action-of-the-hydroethanolic-extract-of-the-flowers-of-acmella-oleracea-l-r-k-jansen-on-the-reproductive-performance-of-wistar-females-rats-a-popular-female-aphrodisiac-from-the-amazon
#14
Clarice Flexa da Rocha, Yuri de Medeiros Souza Lima, Helison Oliveira Carvalho, Rodrigo Costa Pinto, Irlon Maciel Ferreira, Andres Navarrete Castro, Clarissa Silva Lima, José Carlos Tavares Carvalho
ETHNOPHARMACOLOGICAL RELEVANCE: The species Acmella oleracea (L.) R.K. Jansen (Asteraceae), popularly known as jambú, is marketed in fairs as a female aphrodisiac and has several pharmacological activities already confirmed, among them the sexual stimulant action. The objective of this study was to evaluate the effects of the oral administration of the hydroethanolic extract of A. oleracea flowers (EHAo) on wistar rats during the pre-mating, mating, and pre-implantation period. MATERIAL AND METHODS: During the treatment period, measurements of feed intake, water intake, weight, estrous cycle, behavior, reproductive parameters, biochemical parameters, hematological parameters, and histopathology of ovaries were performed daily...
March 25, 2018: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/29262312/effects-of-daily-exposure-to-saccharin-sodium-and-rebaudioside-a-on-the-ovarian-cycle-and-steroidogenesis-in-rats
#15
Jingle Jiang, Lina Qi, Quanwei Wei, Fangxiong Shi
Saccharin sodium and rebaudioside A are widely used as non-caloric sweeteners in our daily life; however, the impacts and regulatory mechanisms of such sweeteners on reproduction remain unclear. In the present study, we used rats as animal models to evaluate the effects of daily exposure to saccharin sodium and rebaudioside A on ovarian biologic functions. Weanling rats were distributed into five experimental groups receiving normal water, 1.5 or 7.5 mM saccharin sodium solution, or 0.5 or 2.5 mM rebaudioside A solution for 48 days of exposure...
December 17, 2017: Reproductive Toxicology
https://www.readbyqxmd.com/read/29261233/evaluation-of-the-estrous-cycle-reproductive-tract-and-mammary-gland-in-female-mice
#16
Justin D Vidal, Adam J Filgo
Evaluation of the female reproductive system is an important part of basic reproductive biology research, toxicology testing, and mutant mouse phenotype assessment. The female reproductive system is dynamic and the onset of puberty and the normal changes observed during estrous cyclicity can create challenges for an investigator. Experimental work in the female mouse requires an understanding of the potential impact of the estrous cycle and tracking normal changes throughout the cycle allows for control of this key variable...
December 20, 2017: Current Protocols in Mouse Biology
https://www.readbyqxmd.com/read/29258619/effects-of-low-intensity-pulsed-ultrasound-lipus-pretreated-human-amnion-derived-mesenchymal-stem-cell-had-msc-transplantation-on-primary-ovarian-insufficiency-in-rats
#17
Li Ling, Xiushan Feng, Tianqin Wei, Yan Wang, Yaping Wang, Wenqian Zhang, Lianli He, Ziling Wang, Qianru Zeng, Zhengai Xiong
BACKGROUND: Human amnion-derived mesenchymal stem cells (hAD-MSCs) have the features of mesenchymal stem cells (MSCs). Low-intensity pulsed ultrasound (LIPUS) can promote the expression of various growth factors and anti-inflammatory molecules that are necessary to keep the follicle growing and to reduce granulosa cell (GC) apoptosis in the ovary. This study aims to explore the effects of LIPUS-pretreated hAD-MSC transplantation on chemotherapy-induced primary ovarian insufficiency (POI) in rats...
December 19, 2017: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29249221/sex-hormones-regulate-rat-hepatic-monocarboxylate-transporter-expression-and-membrane-trafficking
#18
Jieyun Cao, Michael Ng, Melanie A Felmlee
PURPOSE: Monocarboxylate transporters (MCTs) are involved in the transport of monocarboxylates such as ketone bodies, lactate, and pharmaceutical agents. CD147 functions as an ancillary protein for MCT1 and MCT4 for plasma membrane trafficking. Sex differences in MCT1 and MCT4 have been observed in muscle and reproductive tissues; however, there is a paucity of information on MCT sex differences in tissues involved in drug disposition. The objective of the present study was to quantify hepatic MCT1, MCT4 and CD147 mRNA, total cellular and membrane protein expression in males, over the estrous cycle in females and in ovariectomized (OVX) females...
2017: Journal of Pharmacy & Pharmaceutical Sciences: a Publication of the Canadian Society for Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29243395/species-comparison-of-postnatal-development-of-the-female-reproductive-system
#19
REVIEW
Susan B Laffan, Lorraine M Posobiec, Jenny E Uhl, Justin D Vidal
The postnatal development of the female reproductive system in laboratory animals and humans is reviewed. To enable a meaningful species comparison of the developing female reproductive system, common definitions of developmental processes were established with a focus made on aspects that are similar across species. A species comparison of the key endocrine, morphologic, and functional (onset of ovarian cycles and ability to reproduce) features of postnatal development of the female reproductive system is provided for human, nonhuman primate, dog, rat, and also mouse, minipig, and rabbit where possible...
December 15, 2017: Birth Defects Research
https://www.readbyqxmd.com/read/29228129/prenatal-exposure-to-di-2-ethylhexyl-phthalate-causes-long-term-transgenerational-effects-on-female-reproduction-in-mice
#20
Emily Brehm, Saniya Rattan, Liying Gao, Jodi A Flaws
Di(2-ethylhexyl) phthalate (DEHP) is a plasticizer in many consumer products. Although DEHP is a known endocrine disruptor, little is known about the effects of DEHP exposure on female reproduction. Thus, this study tested the hypothesis that prenatal DEHP exposure affects follicle numbers, estrous cyclicity, and hormone levels in multiple generations of mice. Pregnant CD-1 mice were orally dosed with corn oil (vehicle control) or DEHP (20 and 200 µg/kg/day, and 500 and 750 mg/kg/day) from gestation day 10...
December 7, 2017: Endocrinology
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