Leena Strauss, Arttu Junnila, Anni Wärri, Maria Manti, Yiwen Jiang, Eliisa Löyttyniemi, Elisabet Stener-Victorin, Marie K Lagerquist, Krisztina Kukoricza, Taija Heinosalo, Sami Blom, Matti Poutanen
The mouse estrous cycle is divided into four stages: proestrus (P), estrus (E), metestrus (M) and diestrus (D). The estrous cycle affects reproductive hormone levels in a wide variety of tissues. Therefore, to obtain reliable results from female mice, it is important to know the estrous cycle stage during sampling. The stage can be analyzed from a vaginal smear under a microscope. However, it is time-consuming, and the results vary between evaluators. Here, we present an accurate and reproducible method for staging the mouse estrous cycle in digital whole slide images (WSIs) of vaginal smears...
April 1, 2024: Journal of Endocrinology